HCN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCN2
Entrez Gene ID	610
Full Name	hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
Synonyms	BCNG-2,BCNG2,HAC-1
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 brain cyclic nucleotide-gated channel 2 hyperpolarization activated cyclic nucleotide gated potassium channel 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	The protein encoded by this gene is a hyperpolarization-activated cation channel involved in the generation of native pacemaker activity in the heart and in the brain. The encoded protein is activated by cAMP and can produce a fast, large current. Defects in this gene were noted as a possible cause of some forms of epilepsy. [provided by RefSeq, Jan 2017].
Disorder MIM:	602781

mRNA and Protein(s)

mRNA	Protein	Name
NM_001194.3	NP_001185.3	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
NM_001194.4	NP_001185.3	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN2	NP_001185.3
Gallus gallus (chicken)	HCN2	XP_003642915.1
Xenopus tropicalis (tropical clawed frog)	hcn2	XP_002939815.2
Canis lupus familiaris (dog)	HCN2	XP_005642548.1
Rattus norvegicus (Norway rat)	Hcn2	NP_446136.1
Pan troglodytes (chimpanzee)	LOC467640	XP_003952669.1
Bos taurus (cattle)	HCN2	XP_002689207.2
Mus musculus (house mouse)	Hcn2	NP_032252.1
Danio rerio (zebrafish)	hcn2b	XP_685414.3

Gene Ontology
Bibliography

Related articles in PubMed

Gain-of-function HCN2 variants in genetic epilepsy.
Li M, Maljevic S, Phillips AM, Petrovski S, Hildebrand MS, Burgess R, Mount T, Zara F, Striano P, Schubert J, Thiele H, N?rnberg P, Wong M, Weisenberg JL, Thio LL, Lerche H, Scheffer IE, Berkovic SF, Petrou S, Reid CA
Human mutation39(2)202-209(2018 Feb)

Dual expression of constitutively active G?
Nakashima N, Nakashima K, Nakayama T, Takaku A, Kanamori R
Biochemical and biophysical research communications494(1-2)76-81(2017 12)

Case-control pharmacogenetic study of HCN1/HCN2 variants and genetic generalized epilepsies.
Wu SZ, Ye H, Yang XG, Lu ZL, Qu Q, Qu J
Clinical and experimental pharmacology & physiology(2017 Oct)

Mechanism for the inhibition of the cAMP dependence of HCN ion channels by the auxiliary subunit TRIP8b.
Bankston JR, DeBerg HA, Stoll S, Zagotta WN
The Journal of biological chemistry292(43)17794-17803(2017 10)

Maturation and processing of the amyloid precursor protein is regulated by the potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2).
Frykman S, Inoue M, Ikeda A, Teranishi Y, Kihara T, Lundgren JL, Yamamoto NG, Bogdanovic N, Winblad B, Schedin-Weiss S, Tjernberg LO
Biochemical and biophysical research communications483(1)352-358(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta2-adrenoceptors alone strongly enhanced the activity of HCN2 channels, and co-expression of odorant receptors further diversified the HCN2 channel activity.
Title: Dual expression of constitutively active G?

TRIP8b competes with a portion of the cAMP-binding site or distorts the binding site by making interactions with the binding pocket, thus acting predominantly as a competitive antagonist that inhibits the cyclic-nucleotide dependence of HCN channels.
Title: Mechanism for the inhibition of the cAMP dependence of HCN ion channels by the auxiliary subunit TRIP8b.

HCN2 has a role in maturation and processing of the amyloid precursor protein
Title: Maturation and processing of the amyloid precursor protein is regulated by the potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2).

promoter methylation status does not appear to be a major determinant of HCN2 expression in normal and adenoid cystic carcinoma ( ACC )tissues. HCN2 hypomethylation is a biomarker of ACC and may play an important role in the carcinogenesis of ACC.
Title: Identification of methylated genes in salivary gland adenoid cystic carcinoma xenografts using global demethylation and methylation microarray screening.

Aged patients with sinus rhythm exhibited significantly higher expression levels of HCN2 and HCN4 channel mRNA and protein (P<0.05), but significantly lower expression levels of miR1 and 133, compared with those of adult patients with sinus rhythm.
Title: Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation.

The following HCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02807	NM_001194.4 Latest version!	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OHu02807	NM_001194.3	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCN2 ( NM_001194.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185.3 Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.

Price & Availability↑

CloneID	OHu02807
Clone ID Related Accession (Same CDS sequence)	NM_001194.3 , NM_001194.4
Accession Version	NM_001194.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ012582.1, BC039619.1 and AF065164.2. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2007 this sequence version replaced NM_001194.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ012582.1, AF065164.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC GCGGGGGCGG CGGGCGGCCC GGGGAGAGCC CGGGCGCGAC CCCCGCGCCG 
GGGCCGCCGC CGCCGCCGCC GCCCGCGCCC CCCCAACAGC AGCCGCCGCC GCCGCCGCCG 
CCCGCGCCCC CCCCGGGCCC CGGGCCCGCG CCCCCCCAGC ACCCGCCCCG GGCCGAGGCG 
TTGCCCCCGG AGGCGGCGGA TGAGGGCGGC CCGCGGGGCC GGCTCCGCAG CCGCGACAGC 
TCGTGCGGCC GCCCCGGCAC CCCGGGCGCG GCGAGCACGG CCAAGGGCAG CCCGAACGGC 
GAGTGCGGGC GCGGCGAGCC GCAGTGCAGC CCCGCGGGGC CCGAGGGCCC GGCGCGGGGG 
CCCAAGGTGT CGTTCTCGTG CCGCGGGGCG GCCTCGGGGC CCGCGCCGGG GCCGGGGCCG 
GCGGAGGAGG CGGGCAGCGA GGAGGCGGGC CCGGCGGGGG AGCCGCGCGG CAGCCAGGCC 
AGCTTCATGC AGCGCCAGTT CGGCGCGCTC CTGCAGCCGG GCGTCAACAA GTTCTCGCTG 
CGGATGTTCG GCAGCCAGAA GGCCGTGGAG CGCGAGCAGG AGCGCGTCAA GTCGGCGGGG 
GCCTGGATCA TCCACCCGTA CAGCGACTTC AGGTTCTACT GGGACTTCAC CATGCTGCTG 
TTCATGGTGG GAAACCTCAT CATCATCCCA GTGGGCATCA CCTTCTTCAA GGATGAGACC 
ACTGCCCCGT GGATCGTGTT CAACGTGGTC TCGGACACCT TCTTCCTCAT GGACCTGGTG 
TTGAACTTCC GCACCGGCAT TGTGATCGAG GACAACACGG AGATCATCCT GGACCCCGAG 
AAGATCAAGA AGAAGTATCT GCGCACGTGG TTCGTGGTGG ACTTCGTGTC CTCCATCCCC 
GTGGACTACA TCTTCCTTAT CGTGGAGAAG GGCATTGACT CCGAGGTCTA CAAGACGGCA 
CGCGCCCTGC GCATCGTGCG CTTCACCAAG ATCCTCAGCC TCCTGCGGCT GCTGCGCCTC 
TCACGCCTGA TCCGCTACAT CCATCAGTGG GAGGAGATCT TCCACATGAC CTATGACCTG 
GCCAGCGCGG TGATGAGGAT CTGCAATCTC ATCAGCATGA TGCTGCTGCT CTGCCACTGG 
GACGGCTGCC TGCAGTTCCT GGTGCCTATG CTGCAGGACT TCCCGCGCAA CTGCTGGGTG 
TCCATCAATG GCATGGTGAA CCACTCGTGG AGTGAACTGT ACTCCTTCGC ACTCTTCAAG 
GCCATGAGCC ACATGCTGTG CATCGGGTAC GGCCGGCAGG CGCCCGAGAG CATGACGGAC 
ATCTGGCTGA CCATGCTCAG CATGATTGTG GGTGCCACCT GCTACGCCAT GTTCATCGGC 
CACGCCACTG CCCTCATCCA GTCGCTGGAC TCCTCGCGGC GCCAGTACCA GGAGAAGTAC 
AAGCAGGTGG AGCAGTACAT GTCCTTCCAC AAGCTGCCAG CTGACTTCCG CCAGAAGATC 
CACGACTACT ATGAGCACCG TTACCAGGGC AAGATGTTTG ACGAGGACAG CATCCTGGGC 
GAGCTCAACG GGCCCCTGCG GGAGGAGATC GTCAACTTCA ACTGCCGGAA GCTGGTGGCC 
TCCATGCCGC TGTTCGCCAA CGCCGACCCC AACTTCGTCA CGGCCATGCT GACCAAGCTC 
AAGTTCGAGG TCTTCCAGCC GGGTGACTAC ATCATCCGCG AAGGCACCAT CGGGAAGAAG 
ATGTACTTCA TCCAGCACGG CGTGGTCAGC GTGCTCACTA AGGGCAACAA GGAGATGAAG 
CTGTCCGATG GCTCCTACTT CGGGGAGATC TGCCTGCTCA CCCGGGGCCG CCGCACGGCG 
AGCGTGCGGG CTGACACCTA CTGCCGCCTC TATTCGCTGA GCGTGGACAA CTTCAACGAG 
GTGCTGGAGG AGTACCCCAT GATGCGGCGC GCCTTCGAGA CGGTGGCCAT CGACCGCCTG 
GACCGCATCG GCAAGAAGAA TTCCATCCTC CTGCACAAGG TGCAGCATGA CCTCAACTCG 
GGCGTATTCA ACAACCAGGA GAACGCCATC ATCCAGGAGA TCGTCAAGTA CGACCGCGAG 
ATGGTGCAGC AGGCCGAGCT GGGTCAGCGC GTGGGCCTCT TCCCGCCGCC GCCGCCGCCG 
CCGCAGGTCA CCTCGGCCAT CGCCACGCTG CAGCAGGCGG CGGCCATGAG CTTCTGCCCG 
CAGGTGGCGC GGCCGCTCGT GGGGCCGCTG GCGCTCGGCT CGCCGCGCCT CGTGCGCCGC 
CCGCCCCCGG GGCCCGCACC TGCCGCCGCC TCACCCGGGC CCCCGCCCCC CGCCAGCCCC 
CCGGGCGCGC CCGCCAGCCC CCGGGCACCG CGGACCTCGC CCTACGGCGG CCTGCCCGCC 
GCCCCCCTTG CTGGGCCCGC CCTGCCCGCG CGCCGCCTGA GCCGCGCGTC GCGCCCACTG 
TCCGCCTCGC AGCCCTCGCT GCCTCACGGC GCCCCCGGCC CCGCGGCCTC CACACGCCCG 
GCCAGCAGCT CCACACCGCG CTTGGGGCCC ACGCCCGCTG CCCGGGCCGC CGCGCCCAGC 
CCGGACCGCA GGGACTCGGC CTCACCCGGC GCCGCCGGCG GCCTGGACCC CCAGGACTCC 
GCGCGCTCGC GCCTCTCGTC CAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185.3
CDS	54..2723
Translation	MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL

Target ORF information:

RefSeq Version	NM_001194.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194.3

ORF Insert Sequence:

ATGGACGCGC GCGGGGGCGG CGGGCGGCCC GGGGAGAGCC CGGGCGCGAC CCCCGCGCCG 
GGGCCGCCGC CGCCGCCGCC GCCCGCGCCC CCCCAACAGC AGCCGCCGCC GCCGCCGCCG 
CCCGCGCCCC CCCCGGGCCC CGGGCCCGCG CCCCCCCAGC ACCCGCCCCG GGCCGAGGCG 
TTGCCCCCGG AGGCGGCGGA TGAGGGCGGC CCGCGGGGCC GGCTCCGCAG CCGCGACAGC 
TCGTGCGGCC GCCCCGGCAC CCCGGGCGCG GCGAGCACGG CCAAGGGCAG CCCGAACGGC 
GAGTGCGGGC GCGGCGAGCC GCAGTGCAGC CCCGCGGGGC CCGAGGGCCC GGCGCGGGGG 
CCCAAGGTGT CGTTCTCGTG CCGCGGGGCG GCCTCGGGGC CCGCGCCGGG GCCGGGGCCG 
GCGGAGGAGG CGGGCAGCGA GGAGGCGGGC CCGGCGGGGG AGCCGCGCGG CAGCCAGGCC 
AGCTTCATGC AGCGCCAGTT CGGCGCGCTC CTGCAGCCGG GCGTCAACAA GTTCTCGCTG 
CGGATGTTCG GCAGCCAGAA GGCCGTGGAG CGCGAGCAGG AGCGCGTCAA GTCGGCGGGG 
GCCTGGATCA TCCACCCGTA CAGCGACTTC AGGTTCTACT GGGACTTCAC CATGCTGCTG 
TTCATGGTGG GAAACCTCAT CATCATCCCA GTGGGCATCA CCTTCTTCAA GGATGAGACC 
ACTGCCCCGT GGATCGTGTT CAACGTGGTC TCGGACACCT TCTTCCTCAT GGACCTGGTG 
TTGAACTTCC GCACCGGCAT TGTGATCGAG GACAACACGG AGATCATCCT GGACCCCGAG 
AAGATCAAGA AGAAGTATCT GCGCACGTGG TTCGTGGTGG ACTTCGTGTC CTCCATCCCC 
GTGGACTACA TCTTCCTTAT CGTGGAGAAG GGCATTGACT CCGAGGTCTA CAAGACGGCA 
CGCGCCCTGC GCATCGTGCG CTTCACCAAG ATCCTCAGCC TCCTGCGGCT GCTGCGCCTC 
TCACGCCTGA TCCGCTACAT CCATCAGTGG GAGGAGATCT TCCACATGAC CTATGACCTG 
GCCAGCGCGG TGATGAGGAT CTGCAATCTC ATCAGCATGA TGCTGCTGCT CTGCCACTGG 
GACGGCTGCC TGCAGTTCCT GGTGCCTATG CTGCAGGACT TCCCGCGCAA CTGCTGGGTG 
TCCATCAATG GCATGGTGAA CCACTCGTGG AGTGAACTGT ACTCCTTCGC ACTCTTCAAG 
GCCATGAGCC ACATGCTGTG CATCGGGTAC GGCCGGCAGG CGCCCGAGAG CATGACGGAC 
ATCTGGCTGA CCATGCTCAG CATGATTGTG GGTGCCACCT GCTACGCCAT GTTCATCGGC 
CACGCCACTG CCCTCATCCA GTCGCTGGAC TCCTCGCGGC GCCAGTACCA GGAGAAGTAC 
AAGCAGGTGG AGCAGTACAT GTCCTTCCAC AAGCTGCCAG CTGACTTCCG CCAGAAGATC 
CACGACTACT ATGAGCACCG TTACCAGGGC AAGATGTTTG ACGAGGACAG CATCCTGGGC 
GAGCTCAACG GGCCCCTGCG GGAGGAGATC GTCAACTTCA ACTGCCGGAA GCTGGTGGCC 
TCCATGCCGC TGTTCGCCAA CGCCGACCCC AACTTCGTCA CGGCCATGCT GACCAAGCTC 
AAGTTCGAGG TCTTCCAGCC GGGTGACTAC ATCATCCGCG AAGGCACCAT CGGGAAGAAG 
ATGTACTTCA TCCAGCACGG CGTGGTCAGC GTGCTCACTA AGGGCAACAA GGAGATGAAG 
CTGTCCGATG GCTCCTACTT CGGGGAGATC TGCCTGCTCA CCCGGGGCCG CCGCACGGCG 
AGCGTGCGGG CTGACACCTA CTGCCGCCTC TATTCGCTGA GCGTGGACAA CTTCAACGAG 
GTGCTGGAGG AGTACCCCAT GATGCGGCGC GCCTTCGAGA CGGTGGCCAT CGACCGCCTG 
GACCGCATCG GCAAGAAGAA TTCCATCCTC CTGCACAAGG TGCAGCATGA CCTCAACTCG 
GGCGTATTCA ACAACCAGGA GAACGCCATC ATCCAGGAGA TCGTCAAGTA CGACCGCGAG 
ATGGTGCAGC AGGCCGAGCT GGGTCAGCGC GTGGGCCTCT TCCCGCCGCC GCCGCCGCCG 
CCGCAGGTCA CCTCGGCCAT CGCCACGCTG CAGCAGGCGG CGGCCATGAG CTTCTGCCCG 
CAGGTGGCGC GGCCGCTCGT GGGGCCGCTG GCGCTCGGCT CGCCGCGCCT CGTGCGCCGC 
CCGCCCCCGG GGCCCGCACC TGCCGCCGCC TCACCCGGGC CCCCGCCCCC CGCCAGCCCC 
CCGGGCGCGC CCGCCAGCCC CCGGGCACCG CGGACCTCGC CCTACGGCGG CCTGCCCGCC 
GCCCCCCTTG CTGGGCCCGC CCTGCCCGCG CGCCGCCTGA GCCGCGCGTC GCGCCCACTG 
TCCGCCTCGC AGCCCTCGCT GCCTCACGGC GCCCCCGGCC CCGCGGCCTC CACACGCCCG 
GCCAGCAGCT CCACACCGCG CTTGGGGCCC ACGCCCGCTG CCCGGGCCGC CGCGCCCAGC 
CCGGACCGCA GGGACTCGGC CTCACCCGGC GCCGCCGGCG GCCTGGACCC CCAGGACTCC 
GCGCGCTCGC GCCTCTCGTC CAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN2 ( NM_001194.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185.3 Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.

Price & Availability↑

CloneID	OHu02807
Clone ID Related Accession (Same CDS sequence)	NM_001194.3 , NM_001194.4
Accession Version	NM_001194.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005559.2, AJ012582.1, BC039619.1 and AF065164.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001194.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ012582.1, AF065164.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000251287.3/ ENSP00000251287.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGCGC GCGGGGGCGG CGGGCGGCCC GGGGAGAGCC CGGGCGCGAC CCCCGCGCCG 
GGGCCGCCGC CGCCGCCGCC GCCCGCGCCC CCCCAACAGC AGCCGCCGCC GCCGCCGCCG 
CCCGCGCCCC CCCCGGGCCC CGGGCCCGCG CCCCCCCAGC ACCCGCCCCG GGCCGAGGCG 
TTGCCCCCGG AGGCGGCGGA TGAGGGCGGC CCGCGGGGCC GGCTCCGCAG CCGCGACAGC 
TCGTGCGGCC GCCCCGGCAC CCCGGGCGCG GCGAGCACGG CCAAGGGCAG CCCGAACGGC 
GAGTGCGGGC GCGGCGAGCC GCAGTGCAGC CCCGCGGGGC CCGAGGGCCC GGCGCGGGGG 
CCCAAGGTGT CGTTCTCGTG CCGCGGGGCG GCCTCGGGGC CCGCGCCGGG GCCGGGGCCG 
GCGGAGGAGG CGGGCAGCGA GGAGGCGGGC CCGGCGGGGG AGCCGCGCGG CAGCCAGGCC 
AGCTTCATGC AGCGCCAGTT CGGCGCGCTC CTGCAGCCGG GCGTCAACAA GTTCTCGCTG 
CGGATGTTCG GCAGCCAGAA GGCCGTGGAG CGCGAGCAGG AGCGCGTCAA GTCGGCGGGG 
GCCTGGATCA TCCACCCGTA CAGCGACTTC AGGTTCTACT GGGACTTCAC CATGCTGCTG 
TTCATGGTGG GAAACCTCAT CATCATCCCA GTGGGCATCA CCTTCTTCAA GGATGAGACC 
ACTGCCCCGT GGATCGTGTT CAACGTGGTC TCGGACACCT TCTTCCTCAT GGACCTGGTG 
TTGAACTTCC GCACCGGCAT TGTGATCGAG GACAACACGG AGATCATCCT GGACCCCGAG 
AAGATCAAGA AGAAGTATCT GCGCACGTGG TTCGTGGTGG ACTTCGTGTC CTCCATCCCC 
GTGGACTACA TCTTCCTTAT CGTGGAGAAG GGCATTGACT CCGAGGTCTA CAAGACGGCA 
CGCGCCCTGC GCATCGTGCG CTTCACCAAG ATCCTCAGCC TCCTGCGGCT GCTGCGCCTC 
TCACGCCTGA TCCGCTACAT CCATCAGTGG GAGGAGATCT TCCACATGAC CTATGACCTG 
GCCAGCGCGG TGATGAGGAT CTGCAATCTC ATCAGCATGA TGCTGCTGCT CTGCCACTGG 
GACGGCTGCC TGCAGTTCCT GGTGCCTATG CTGCAGGACT TCCCGCGCAA CTGCTGGGTG 
TCCATCAATG GCATGGTGAA CCACTCGTGG AGTGAACTGT ACTCCTTCGC ACTCTTCAAG 
GCCATGAGCC ACATGCTGTG CATCGGGTAC GGCCGGCAGG CGCCCGAGAG CATGACGGAC 
ATCTGGCTGA CCATGCTCAG CATGATTGTG GGTGCCACCT GCTACGCCAT GTTCATCGGC 
CACGCCACTG CCCTCATCCA GTCGCTGGAC TCCTCGCGGC GCCAGTACCA GGAGAAGTAC 
AAGCAGGTGG AGCAGTACAT GTCCTTCCAC AAGCTGCCAG CTGACTTCCG CCAGAAGATC 
CACGACTACT ATGAGCACCG TTACCAGGGC AAGATGTTTG ACGAGGACAG CATCCTGGGC 
GAGCTCAACG GGCCCCTGCG GGAGGAGATC GTCAACTTCA ACTGCCGGAA GCTGGTGGCC 
TCCATGCCGC TGTTCGCCAA CGCCGACCCC AACTTCGTCA CGGCCATGCT GACCAAGCTC 
AAGTTCGAGG TCTTCCAGCC GGGTGACTAC ATCATCCGCG AAGGCACCAT CGGGAAGAAG 
ATGTACTTCA TCCAGCACGG CGTGGTCAGC GTGCTCACTA AGGGCAACAA GGAGATGAAG 
CTGTCCGATG GCTCCTACTT CGGGGAGATC TGCCTGCTCA CCCGGGGCCG CCGCACGGCG 
AGCGTGCGGG CTGACACCTA CTGCCGCCTC TATTCGCTGA GCGTGGACAA CTTCAACGAG 
GTGCTGGAGG AGTACCCCAT GATGCGGCGC GCCTTCGAGA CGGTGGCCAT CGACCGCCTG 
GACCGCATCG GCAAGAAGAA TTCCATCCTC CTGCACAAGG TGCAGCATGA CCTCAACTCG 
GGCGTATTCA ACAACCAGGA GAACGCCATC ATCCAGGAGA TCGTCAAGTA CGACCGCGAG 
ATGGTGCAGC AGGCCGAGCT GGGTCAGCGC GTGGGCCTCT TCCCGCCGCC GCCGCCGCCG 
CCGCAGGTCA CCTCGGCCAT CGCCACGCTG CAGCAGGCGG CGGCCATGAG CTTCTGCCCG 
CAGGTGGCGC GGCCGCTCGT GGGGCCGCTG GCGCTCGGCT CGCCGCGCCT CGTGCGCCGC 
CCGCCCCCGG GGCCCGCACC TGCCGCCGCC TCACCCGGGC CCCCGCCCCC CGCCAGCCCC 
CCGGGCGCGC CCGCCAGCCC CCGGGCACCG CGGACCTCGC CCTACGGCGG CCTGCCCGCC 
GCCCCCCTTG CTGGGCCCGC CCTGCCCGCG CGCCGCCTGA GCCGCGCGTC GCGCCCACTG 
TCCGCCTCGC AGCCCTCGCT GCCTCACGGC GCCCCCGGCC CCGCGGCCTC CACACGCCCG 
GCCAGCAGCT CCACACCGCG CTTGGGGCCC ACGCCCGCTG CCCGGGCCGC CGCGCCCAGC 
CCGGACCGCA GGGACTCGGC CTCACCCGGC GCCGCCGGCG GCCTGGACCC CCAGGACTCC 
GCGCGCTCGC GCCTCTCGTC CAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185.3
CDS	66..2735
Translation	MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL

Target ORF information:

RefSeq Version	NM_001194.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 (HCN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001194.4

ORF Insert Sequence:

ATGGACGCGC GCGGGGGCGG CGGGCGGCCC GGGGAGAGCC CGGGCGCGAC CCCCGCGCCG 
GGGCCGCCGC CGCCGCCGCC GCCCGCGCCC CCCCAACAGC AGCCGCCGCC GCCGCCGCCG 
CCCGCGCCCC CCCCGGGCCC CGGGCCCGCG CCCCCCCAGC ACCCGCCCCG GGCCGAGGCG 
TTGCCCCCGG AGGCGGCGGA TGAGGGCGGC CCGCGGGGCC GGCTCCGCAG CCGCGACAGC 
TCGTGCGGCC GCCCCGGCAC CCCGGGCGCG GCGAGCACGG CCAAGGGCAG CCCGAACGGC 
GAGTGCGGGC GCGGCGAGCC GCAGTGCAGC CCCGCGGGGC CCGAGGGCCC GGCGCGGGGG 
CCCAAGGTGT CGTTCTCGTG CCGCGGGGCG GCCTCGGGGC CCGCGCCGGG GCCGGGGCCG 
GCGGAGGAGG CGGGCAGCGA GGAGGCGGGC CCGGCGGGGG AGCCGCGCGG CAGCCAGGCC 
AGCTTCATGC AGCGCCAGTT CGGCGCGCTC CTGCAGCCGG GCGTCAACAA GTTCTCGCTG 
CGGATGTTCG GCAGCCAGAA GGCCGTGGAG CGCGAGCAGG AGCGCGTCAA GTCGGCGGGG 
GCCTGGATCA TCCACCCGTA CAGCGACTTC AGGTTCTACT GGGACTTCAC CATGCTGCTG 
TTCATGGTGG GAAACCTCAT CATCATCCCA GTGGGCATCA CCTTCTTCAA GGATGAGACC 
ACTGCCCCGT GGATCGTGTT CAACGTGGTC TCGGACACCT TCTTCCTCAT GGACCTGGTG 
TTGAACTTCC GCACCGGCAT TGTGATCGAG GACAACACGG AGATCATCCT GGACCCCGAG 
AAGATCAAGA AGAAGTATCT GCGCACGTGG TTCGTGGTGG ACTTCGTGTC CTCCATCCCC 
GTGGACTACA TCTTCCTTAT CGTGGAGAAG GGCATTGACT CCGAGGTCTA CAAGACGGCA 
CGCGCCCTGC GCATCGTGCG CTTCACCAAG ATCCTCAGCC TCCTGCGGCT GCTGCGCCTC 
TCACGCCTGA TCCGCTACAT CCATCAGTGG GAGGAGATCT TCCACATGAC CTATGACCTG 
GCCAGCGCGG TGATGAGGAT CTGCAATCTC ATCAGCATGA TGCTGCTGCT CTGCCACTGG 
GACGGCTGCC TGCAGTTCCT GGTGCCTATG CTGCAGGACT TCCCGCGCAA CTGCTGGGTG 
TCCATCAATG GCATGGTGAA CCACTCGTGG AGTGAACTGT ACTCCTTCGC ACTCTTCAAG 
GCCATGAGCC ACATGCTGTG CATCGGGTAC GGCCGGCAGG CGCCCGAGAG CATGACGGAC 
ATCTGGCTGA CCATGCTCAG CATGATTGTG GGTGCCACCT GCTACGCCAT GTTCATCGGC 
CACGCCACTG CCCTCATCCA GTCGCTGGAC TCCTCGCGGC GCCAGTACCA GGAGAAGTAC 
AAGCAGGTGG AGCAGTACAT GTCCTTCCAC AAGCTGCCAG CTGACTTCCG CCAGAAGATC 
CACGACTACT ATGAGCACCG TTACCAGGGC AAGATGTTTG ACGAGGACAG CATCCTGGGC 
GAGCTCAACG GGCCCCTGCG GGAGGAGATC GTCAACTTCA ACTGCCGGAA GCTGGTGGCC 
TCCATGCCGC TGTTCGCCAA CGCCGACCCC AACTTCGTCA CGGCCATGCT GACCAAGCTC 
AAGTTCGAGG TCTTCCAGCC GGGTGACTAC ATCATCCGCG AAGGCACCAT CGGGAAGAAG 
ATGTACTTCA TCCAGCACGG CGTGGTCAGC GTGCTCACTA AGGGCAACAA GGAGATGAAG 
CTGTCCGATG GCTCCTACTT CGGGGAGATC TGCCTGCTCA CCCGGGGCCG CCGCACGGCG 
AGCGTGCGGG CTGACACCTA CTGCCGCCTC TATTCGCTGA GCGTGGACAA CTTCAACGAG 
GTGCTGGAGG AGTACCCCAT GATGCGGCGC GCCTTCGAGA CGGTGGCCAT CGACCGCCTG 
GACCGCATCG GCAAGAAGAA TTCCATCCTC CTGCACAAGG TGCAGCATGA CCTCAACTCG 
GGCGTATTCA ACAACCAGGA GAACGCCATC ATCCAGGAGA TCGTCAAGTA CGACCGCGAG 
ATGGTGCAGC AGGCCGAGCT GGGTCAGCGC GTGGGCCTCT TCCCGCCGCC GCCGCCGCCG 
CCGCAGGTCA CCTCGGCCAT CGCCACGCTG CAGCAGGCGG CGGCCATGAG CTTCTGCCCG 
CAGGTGGCGC GGCCGCTCGT GGGGCCGCTG GCGCTCGGCT CGCCGCGCCT CGTGCGCCGC 
CCGCCCCCGG GGCCCGCACC TGCCGCCGCC TCACCCGGGC CCCCGCCCCC CGCCAGCCCC 
CCGGGCGCGC CCGCCAGCCC CCGGGCACCG CGGACCTCGC CCTACGGCGG CCTGCCCGCC 
GCCCCCCTTG CTGGGCCCGC CCTGCCCGCG CGCCGCCTGA GCCGCGCGTC GCGCCCACTG 
TCCGCCTCGC AGCCCTCGCT GCCTCACGGC GCCCCCGGCC CCGCGGCCTC CACACGCCCG 
GCCAGCAGCT CCACACCGCG CTTGGGGCCC ACGCCCGCTG CCCGGGCCGC CGCGCCCAGC 
CCGGACCGCA GGGACTCGGC CTCACCCGGC GCCGCCGGCG GCCTGGACCC CCAGGACTCC 
GCGCGCTCGC GCCTCTCGTC CAACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gain-of-function HCN2 variants in genetic epilepsy.
Human mutation39(2)202-209(2018 Feb)
Li M,Maljevic S,Phillips AM,Petrovski S,Hildebrand MS,Burgess R,Mount T,Zara F,Striano P,Schubert J,Thiele H,N?rnberg P,Wong M,Weisenberg JL,Thio LL,Lerche H,Scheffer IE,Berkovic SF,Petrou S,Reid CA

Dual expression of constitutively active G?
Biochemical and biophysical research communications494(1-2)76-81(2017 12)
Nakashima N,Nakashima K,Nakayama T,Takaku A,Kanamori R

Case-control pharmacogenetic study of HCN1/HCN2 variants and genetic generalized epilepsies.
Clinical and experimental pharmacology & physiology(2017 Oct)
Wu SZ,Ye H,Yang XG,Lu ZL,Qu Q,Qu J

Mechanism for the inhibition of the cAMP dependence of HCN ion channels by the auxiliary subunit TRIP8b.
The Journal of biological chemistry292(43)17794-17803(2017 10)
Bankston JR,DeBerg HA,Stoll S,Zagotta WN

Maturation and processing of the amyloid precursor protein is regulated by the potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2).
Biochemical and biophysical research communications483(1)352-358(2017 01)
Frykman S,Inoue M,Ikeda A,Teranishi Y,Kihara T,Lundgren JL,Yamamoto NG,Bogdanovic N,Winblad B,Schedin-Weiss S,Tjernberg LO

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HCN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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