ROCK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK1
Entrez Gene ID	6093
Full Name	Rho associated coiled-coil containing protein kinase 1
Synonyms	P160ROCK,ROCK-I
General protein information	Preferred Names Rho associated coiled-coil containing protein kinase 1 Names rho-associated protein kinase 1 p160 ROCK-1 renal carcinoma antigen NY-REN-35
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18q11.1
Summary	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015].
Disorder MIM:	601702

mRNA and Protein(s)

mRNA	Protein	Name
NM_005406.3	NP_005397.1	rho-associated protein kinase 1
NM_005406.2	NP_005397.1	rho-associated protein kinase 1
XM_011526137.3	XP_011524439.1	rho-associated protein kinase 1 isoform X1
XM_011526137.2	XP_011524439.1	rho-associated protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rock1	NP_001243563.1
Canis lupus familiaris (dog)	ROCK1	XP_005623079.1
Rattus norvegicus (Norway rat)	Rock1	NP_112360.1
Xenopus tropicalis (tropical clawed frog)	rock1	XP_002934180.2
Homo sapiens (human)	ROCK1	NP_005397.1
Pan troglodytes (chimpanzee)	ROCK1	XP_001151982.1
	ROCK1	XP_001091266.1
Bos taurus (cattle)	ROCK1	XP_002697835.2
Mus musculus (house mouse)	Rock1	NP_033097.1
Gallus gallus (chicken)	ROCK1	NP_001186377.1

Gene Ontology

The following ROCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25585	NM_005406.3 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu60286	XM_011526137.3 Latest version!	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
View Old Accession Versions >>
OHu25585	NM_005406.2	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu60286	XM_011526137.2	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK1 ( NM_005406.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005397.1 Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Price & Availability↑

CloneID	OHu25585
Clone ID Related Accession (Same CDS sequence)	NM_005406.3 , NM_005406.2
Accession Version	NM_005406.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057038.1, U43195.1, AU121527.1 and AC036178.11. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005406.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.247054.1, SRR1803614.28920.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000399799.3/ ENSP00000382697.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGACTG GGGACAGTTT TGAGACTCGA TTTGAAAAAA TGGACAACCT GCTGCGGGAT 
CCCAAATCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA 
GACACAATAA ATAAAATCAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG 
GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG 
GTATATGCTA TGAAGCTTCT CAGCAAATTT GAAATGATAA AGAGATCTGA TTCTGCTTTT 
TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCCAACAGTC CTTGGGTTGT TCAGCTTTTT 
TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT 
CTTGTAAACT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA 
GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCACAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT 
ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CGGTTGGAAC ACCTGATTAT 
ATTTCCCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGTT ATTATGGAAG AGAATGTGAC 
TGGTGGTCGG TTGGGGTATT TTTATACGAA ATGCTTGTAG GTGATACACC TTTTTATGCA 
GATTCTTTGG TTGGAACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACCTTTCCT 
GATGATAATG ACATATCAAA AGAAGCAAAA AACCTTATTT GTGCCTTCCT TACTGACAGG 
GAAGTGAGGT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GACCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTACC CGATTTAAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA GGAAGAAACA 
TTCCCTATTC CTAAAGCTTT CGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACAGAACTAG CTCCAATGCA 
GATAAAAGCT TGCAGGAAAG TTTGCAAAAA ACAATCTATA AGCTGGAAGA ACAGCTGCAT 
AATGAAATGC AGTTAAAAGA TGAAATGGAG CAGAAGTGCA GAACCTCAAA CATAAAACTA 
GACAAGATAA TGAAAGAATT GGATGAAGAG GGAAATCAAA GAAGAAATCT AGAATCTACA 
GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAAAGA 
AAAGCTGAAC AGGAAAATGA GAAGAGAAGA AATGTAGAAA ATGAAGTTTC TACATTAAAG 
GATCAGTTGG AAGACTTAAA GAAAGTCAGT CAGAATTCAC AGCTTGCTAA TGAGAAGCTG 
TCCCAGTTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCGGACACA 
GCTGTAAGAT TGAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GTTAGAGTCC 
CTGAACAGAG AGTTGCAAGA GAGAAATCGA ATTTTAGAGA ATTCTAAGTC ACAAACAGAC 
AAAGATTATT ACCAGCTGCA AGCTATATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT 
TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT 
CTCAAACATA ATCTCGAAAA AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT 
CACTCAGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTACAAACT TAAATCATTA 
CAACAACGGT TAGAACAAGA GGTAAATGAA CACAAAGTAA CCAAAGCTCG TTTAACTGAC 
AAACATCAAT CTATTGAAGA GGCAAAGTCT GTGGCAATGT GTGAGATGGA AAAAAAGCTG 
AAAGAAGAAA GAGAAGCTCG AGAGAAGGCT GAAAATCGGG TTGTTCAGAT TGAGAAACAG 
TGTTCCATGC TAGACGTTGA TCTGAAGCAA TCTCAGCAGA AACTAGAACA TTTGACTGGA 
AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTAACCC TGCAACTGGA GCAGGAATCA 
AATAAGCGGC TGTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA 
AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA 
TTAGAATTTG AGTTAGCTCA GCTTACGAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG 
GAGCTACAAG ATCAGCTTGA AGCTGAGCAA TATTTCTCGA CACTTTATAA AACCCAGGTA 
AAGGAACTTA AAGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAGGAA 
CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAAGCTGAG 
TCTGAGCAGT TGGCGCGAGG CCTTCTGGAA GAACAGTATT TTGAATTGAC GCAAGAAAGC 
AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG 
CTTGAAGAAG CAAACAGCAT GCTAACCAAA GATATTGAAA TATTAAGAAG AGAGAATGAA 
GAGCTAACAG AGAAAATGAA GAAGGCAGAG GAAGAATATA AACTGGAGAA GGAGGAGGAG 
ATCAGTAATC TTAAGGCTGC CTTTGAAAAG AATATCAACA CTGAACGAAC CCTTAAAACA 
CAGGCTGTTA ACAAATTGGC AGAAATAATG AATCGAAAAG ATTTTAAAAT TGATAGAAAG 
AAAGCTAATA CACAAGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT GCAACTGGAA 
CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTAGTGA AACATCAGAA GGAACTGAAT 
GACATGCAAG CGCAATTGGT AGAAGAATGT GCACATAGGA ATGAGCTTCA GATGCAGTTG 
GCCAGCAAAG AGAGTGATAT TGAGCAATTG CGTGCTAAAC TTTTGGACCT CTCGGATTCT 
ACAAGTGTTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GTAACCTCCC AGAGTCAAGA 
ATTGAAGGTT GGCTTTCAGT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA 
CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGACGAACA AGATAAGGAG 
CAATCCAATC CATCTATGGT ATTGGACATA GATAAACTGT TTCACGTTAG ACCTGTAACC 
CAAGGAGATG TGTATAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT 
GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAA 
ACTAATTTCC AAAATCACAA AGGCCATGAG TTTATTCCTA CACTCTACCA CTTTCCTGCC 
AATTGTGATG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTAGAG 
TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGACTTAATT 
TGTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT 
TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAAATCCC TAAGAATCCA 
CCATCTGGTT TTGTTCGTGC TTCCCCTCGA ACGCTTTCTA CAAGATCCAC TGCAAATCAG 
TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAACTA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005397.1
CDS	904..4968
Translation	MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	NM_005406.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005406.3

ORF Insert Sequence:

ATGTCGACTG GGGACAGTTT TGAGACTCGA TTTGAAAAAA TGGACAACCT GCTGCGGGAT 
CCCAAATCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA 
GACACAATAA ATAAAATCAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG 
GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG 
GTATATGCTA TGAAGCTTCT CAGCAAATTT GAAATGATAA AGAGATCTGA TTCTGCTTTT 
TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCCAACAGTC CTTGGGTTGT TCAGCTTTTT 
TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT 
CTTGTAAACT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA 
GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCACAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT 
ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CGGTTGGAAC ACCTGATTAT 
ATTTCCCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGTT ATTATGGAAG AGAATGTGAC 
TGGTGGTCGG TTGGGGTATT TTTATACGAA ATGCTTGTAG GTGATACACC TTTTTATGCA 
GATTCTTTGG TTGGAACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACCTTTCCT 
GATGATAATG ACATATCAAA AGAAGCAAAA AACCTTATTT GTGCCTTCCT TACTGACAGG 
GAAGTGAGGT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GACCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTACC CGATTTAAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA GGAAGAAACA 
TTCCCTATTC CTAAAGCTTT CGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACAGAACTAG CTCCAATGCA 
GATAAAAGCT TGCAGGAAAG TTTGCAAAAA ACAATCTATA AGCTGGAAGA ACAGCTGCAT 
AATGAAATGC AGTTAAAAGA TGAAATGGAG CAGAAGTGCA GAACCTCAAA CATAAAACTA 
GACAAGATAA TGAAAGAATT GGATGAAGAG GGAAATCAAA GAAGAAATCT AGAATCTACA 
GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAAAGA 
AAAGCTGAAC AGGAAAATGA GAAGAGAAGA AATGTAGAAA ATGAAGTTTC TACATTAAAG 
GATCAGTTGG AAGACTTAAA GAAAGTCAGT CAGAATTCAC AGCTTGCTAA TGAGAAGCTG 
TCCCAGTTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCGGACACA 
GCTGTAAGAT TGAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GTTAGAGTCC 
CTGAACAGAG AGTTGCAAGA GAGAAATCGA ATTTTAGAGA ATTCTAAGTC ACAAACAGAC 
AAAGATTATT ACCAGCTGCA AGCTATATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT 
TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT 
CTCAAACATA ATCTCGAAAA AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT 
CACTCAGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTACAAACT TAAATCATTA 
CAACAACGGT TAGAACAAGA GGTAAATGAA CACAAAGTAA CCAAAGCTCG TTTAACTGAC 
AAACATCAAT CTATTGAAGA GGCAAAGTCT GTGGCAATGT GTGAGATGGA AAAAAAGCTG 
AAAGAAGAAA GAGAAGCTCG AGAGAAGGCT GAAAATCGGG TTGTTCAGAT TGAGAAACAG 
TGTTCCATGC TAGACGTTGA TCTGAAGCAA TCTCAGCAGA AACTAGAACA TTTGACTGGA 
AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTAACCC TGCAACTGGA GCAGGAATCA 
AATAAGCGGC TGTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA 
AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA 
TTAGAATTTG AGTTAGCTCA GCTTACGAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG 
GAGCTACAAG ATCAGCTTGA AGCTGAGCAA TATTTCTCGA CACTTTATAA AACCCAGGTA 
AAGGAACTTA AAGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAGGAA 
CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAAGCTGAG 
TCTGAGCAGT TGGCGCGAGG CCTTCTGGAA GAACAGTATT TTGAATTGAC GCAAGAAAGC 
AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG 
CTTGAAGAAG CAAACAGCAT GCTAACCAAA GATATTGAAA TATTAAGAAG AGAGAATGAA 
GAGCTAACAG AGAAAATGAA GAAGGCAGAG GAAGAATATA AACTGGAGAA GGAGGAGGAG 
ATCAGTAATC TTAAGGCTGC CTTTGAAAAG AATATCAACA CTGAACGAAC CCTTAAAACA 
CAGGCTGTTA ACAAATTGGC AGAAATAATG AATCGAAAAG ATTTTAAAAT TGATAGAAAG 
AAAGCTAATA CACAAGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT GCAACTGGAA 
CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTAGTGA AACATCAGAA GGAACTGAAT 
GACATGCAAG CGCAATTGGT AGAAGAATGT GCACATAGGA ATGAGCTTCA GATGCAGTTG 
GCCAGCAAAG AGAGTGATAT TGAGCAATTG CGTGCTAAAC TTTTGGACCT CTCGGATTCT 
ACAAGTGTTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GTAACCTCCC AGAGTCAAGA 
ATTGAAGGTT GGCTTTCAGT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA 
CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGACGAACA AGATAAGGAG 
CAATCCAATC CATCTATGGT ATTGGACATA GATAAACTGT TTCACGTTAG ACCTGTAACC 
CAAGGAGATG TGTATAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT 
GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAA 
ACTAATTTCC AAAATCACAA AGGCCATGAG TTTATTCCTA CACTCTACCA CTTTCCTGCC 
AATTGTGATG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTAGAG 
TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGACTTAATT 
TGTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT 
TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAAATCCC TAAGAATCCA 
CCATCTGGTT TTGTTCGTGC TTCCCCTCGA ACGCTTTCTA CAAGATCCAC TGCAAATCAG 
TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAACTA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( NM_005406.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005397.1 Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Price & Availability↑

CloneID	OHu25585
Clone ID Related Accession (Same CDS sequence)	NM_005406.3 , NM_005406.2
Accession Version	NM_005406.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057038.1, U43195.1, AU121527.1 and AC036178.11. This sequence is a reference standard in the RefSeqGene project. On or before Jun 10, 2008 this sequence version replaced XM_001723570.1, XM_001713904.1, XM_001722015.1, NM_005406.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGACTG GGGACAGTTT TGAGACTCGA TTTGAAAAAA TGGACAACCT GCTGCGGGAT 
CCCAAATCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA 
GACACAATAA ATAAAATCAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG 
GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG 
GTATATGCTA TGAAGCTTCT CAGCAAATTT GAAATGATAA AGAGATCTGA TTCTGCTTTT 
TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCCAACAGTC CTTGGGTTGT TCAGCTTTTT 
TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT 
CTTGTAAACT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA 
GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCACAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT 
ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CGGTTGGAAC ACCTGATTAT 
ATTTCCCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGTT ATTATGGAAG AGAATGTGAC 
TGGTGGTCGG TTGGGGTATT TTTATACGAA ATGCTTGTAG GTGATACACC TTTTTATGCA 
GATTCTTTGG TTGGAACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACCTTTCCT 
GATGATAATG ACATATCAAA AGAAGCAAAA AACCTTATTT GTGCCTTCCT TACTGACAGG 
GAAGTGAGGT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GACCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTACC CGATTTAAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA GGAAGAAACA 
TTCCCTATTC CTAAAGCTTT CGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACAGAACTAG CTCCAATGCA 
GATAAAAGCT TGCAGGAAAG TTTGCAAAAA ACAATCTATA AGCTGGAAGA ACAGCTGCAT 
AATGAAATGC AGTTAAAAGA TGAAATGGAG CAGAAGTGCA GAACCTCAAA CATAAAACTA 
GACAAGATAA TGAAAGAATT GGATGAAGAG GGAAATCAAA GAAGAAATCT AGAATCTACA 
GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAAAGA 
AAAGCTGAAC AGGAAAATGA GAAGAGAAGA AATGTAGAAA ATGAAGTTTC TACATTAAAG 
GATCAGTTGG AAGACTTAAA GAAAGTCAGT CAGAATTCAC AGCTTGCTAA TGAGAAGCTG 
TCCCAGTTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCGGACACA 
GCTGTAAGAT TGAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GTTAGAGTCC 
CTGAACAGAG AGTTGCAAGA GAGAAATCGA ATTTTAGAGA ATTCTAAGTC ACAAACAGAC 
AAAGATTATT ACCAGCTGCA AGCTATATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT 
TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT 
CTCAAACATA ATCTCGAAAA AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT 
CACTCAGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTACAAACT TAAATCATTA 
CAACAACGGT TAGAACAAGA GGTAAATGAA CACAAAGTAA CCAAAGCTCG TTTAACTGAC 
AAACATCAAT CTATTGAAGA GGCAAAGTCT GTGGCAATGT GTGAGATGGA AAAAAAGCTG 
AAAGAAGAAA GAGAAGCTCG AGAGAAGGCT GAAAATCGGG TTGTTCAGAT TGAGAAACAG 
TGTTCCATGC TAGACGTTGA TCTGAAGCAA TCTCAGCAGA AACTAGAACA TTTGACTGGA 
AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTAACCC TGCAACTGGA GCAGGAATCA 
AATAAGCGGC TGTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA 
AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA 
TTAGAATTTG AGTTAGCTCA GCTTACGAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG 
GAGCTACAAG ATCAGCTTGA AGCTGAGCAA TATTTCTCGA CACTTTATAA AACCCAGGTA 
AAGGAACTTA AAGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAGGAA 
CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAAGCTGAG 
TCTGAGCAGT TGGCGCGAGG CCTTCTGGAA GAACAGTATT TTGAATTGAC GCAAGAAAGC 
AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG 
CTTGAAGAAG CAAACAGCAT GCTAACCAAA GATATTGAAA TATTAAGAAG AGAGAATGAA 
GAGCTAACAG AGAAAATGAA GAAGGCAGAG GAAGAATATA AACTGGAGAA GGAGGAGGAG 
ATCAGTAATC TTAAGGCTGC CTTTGAAAAG AATATCAACA CTGAACGAAC CCTTAAAACA 
CAGGCTGTTA ACAAATTGGC AGAAATAATG AATCGAAAAG ATTTTAAAAT TGATAGAAAG 
AAAGCTAATA CACAAGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT GCAACTGGAA 
CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTAGTGA AACATCAGAA GGAACTGAAT 
GACATGCAAG CGCAATTGGT AGAAGAATGT GCACATAGGA ATGAGCTTCA GATGCAGTTG 
GCCAGCAAAG AGAGTGATAT TGAGCAATTG CGTGCTAAAC TTTTGGACCT CTCGGATTCT 
ACAAGTGTTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GTAACCTCCC AGAGTCAAGA 
ATTGAAGGTT GGCTTTCAGT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA 
CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGACGAACA AGATAAGGAG 
CAATCCAATC CATCTATGGT ATTGGACATA GATAAACTGT TTCACGTTAG ACCTGTAACC 
CAAGGAGATG TGTATAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT 
GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAA 
ACTAATTTCC AAAATCACAA AGGCCATGAG TTTATTCCTA CACTCTACCA CTTTCCTGCC 
AATTGTGATG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTAGAG 
TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGACTTAATT 
TGTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT 
TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAAATCCC TAAGAATCCA 
CCATCTGGTT TTGTTCGTGC TTCCCCTCGA ACGCTTTCTA CAAGATCCAC TGCAAATCAG 
TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAACTA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005397.1
CDS	942..5006
Translation	MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	NM_005406.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005406.2

ORF Insert Sequence:

ATGTCGACTG GGGACAGTTT TGAGACTCGA TTTGAAAAAA TGGACAACCT GCTGCGGGAT 
CCCAAATCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 
TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA 
GACACAATAA ATAAAATCAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG 
GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG 
GTATATGCTA TGAAGCTTCT CAGCAAATTT GAAATGATAA AGAGATCTGA TTCTGCTTTT 
TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCCAACAGTC CTTGGGTTGT TCAGCTTTTT 
TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT 
CTTGTAAACT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA 
GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCACAG AGATGTGAAG 
CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT 
ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CGGTTGGAAC ACCTGATTAT 
ATTTCCCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGTT ATTATGGAAG AGAATGTGAC 
TGGTGGTCGG TTGGGGTATT TTTATACGAA ATGCTTGTAG GTGATACACC TTTTTATGCA 
GATTCTTTGG TTGGAACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACCTTTCCT 
GATGATAATG ACATATCAAA AGAAGCAAAA AACCTTATTT GTGCCTTCCT TACTGACAGG 
GAAGTGAGGT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 
GACCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTACC CGATTTAAGT 
AGTGACATTG ATACTAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA GGAAGAAACA 
TTCCCTATTC CTAAAGCTTT CGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 
AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACAGAACTAG CTCCAATGCA 
GATAAAAGCT TGCAGGAAAG TTTGCAAAAA ACAATCTATA AGCTGGAAGA ACAGCTGCAT 
AATGAAATGC AGTTAAAAGA TGAAATGGAG CAGAAGTGCA GAACCTCAAA CATAAAACTA 
GACAAGATAA TGAAAGAATT GGATGAAGAG GGAAATCAAA GAAGAAATCT AGAATCTACA 
GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAAAGA 
AAAGCTGAAC AGGAAAATGA GAAGAGAAGA AATGTAGAAA ATGAAGTTTC TACATTAAAG 
GATCAGTTGG AAGACTTAAA GAAAGTCAGT CAGAATTCAC AGCTTGCTAA TGAGAAGCTG 
TCCCAGTTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCGGACACA 
GCTGTAAGAT TGAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GTTAGAGTCC 
CTGAACAGAG AGTTGCAAGA GAGAAATCGA ATTTTAGAGA ATTCTAAGTC ACAAACAGAC 
AAAGATTATT ACCAGCTGCA AGCTATATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT 
TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT 
CTCAAACATA ATCTCGAAAA AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT 
CACTCAGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTACAAACT TAAATCATTA 
CAACAACGGT TAGAACAAGA GGTAAATGAA CACAAAGTAA CCAAAGCTCG TTTAACTGAC 
AAACATCAAT CTATTGAAGA GGCAAAGTCT GTGGCAATGT GTGAGATGGA AAAAAAGCTG 
AAAGAAGAAA GAGAAGCTCG AGAGAAGGCT GAAAATCGGG TTGTTCAGAT TGAGAAACAG 
TGTTCCATGC TAGACGTTGA TCTGAAGCAA TCTCAGCAGA AACTAGAACA TTTGACTGGA 
AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTAACCC TGCAACTGGA GCAGGAATCA 
AATAAGCGGC TGTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA 
AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA 
TTAGAATTTG AGTTAGCTCA GCTTACGAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG 
GAGCTACAAG ATCAGCTTGA AGCTGAGCAA TATTTCTCGA CACTTTATAA AACCCAGGTA 
AAGGAACTTA AAGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAGGAA 
CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAAGCTGAG 
TCTGAGCAGT TGGCGCGAGG CCTTCTGGAA GAACAGTATT TTGAATTGAC GCAAGAAAGC 
AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG 
CTTGAAGAAG CAAACAGCAT GCTAACCAAA GATATTGAAA TATTAAGAAG AGAGAATGAA 
GAGCTAACAG AGAAAATGAA GAAGGCAGAG GAAGAATATA AACTGGAGAA GGAGGAGGAG 
ATCAGTAATC TTAAGGCTGC CTTTGAAAAG AATATCAACA CTGAACGAAC CCTTAAAACA 
CAGGCTGTTA ACAAATTGGC AGAAATAATG AATCGAAAAG ATTTTAAAAT TGATAGAAAG 
AAAGCTAATA CACAAGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT GCAACTGGAA 
CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTAGTGA AACATCAGAA GGAACTGAAT 
GACATGCAAG CGCAATTGGT AGAAGAATGT GCACATAGGA ATGAGCTTCA GATGCAGTTG 
GCCAGCAAAG AGAGTGATAT TGAGCAATTG CGTGCTAAAC TTTTGGACCT CTCGGATTCT 
ACAAGTGTTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GTAACCTCCC AGAGTCAAGA 
ATTGAAGGTT GGCTTTCAGT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA 
CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGACGAACA AGATAAGGAG 
CAATCCAATC CATCTATGGT ATTGGACATA GATAAACTGT TTCACGTTAG ACCTGTAACC 
CAAGGAGATG TGTATAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT 
GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAA 
ACTAATTTCC AAAATCACAA AGGCCATGAG TTTATTCCTA CACTCTACCA CTTTCCTGCC 
AATTGTGATG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTAGAG 
TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGACTTAATT 
TGTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT 
TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAAATCCC TAAGAATCCA 
CCATCTGGTT TTGTTCGTGC TTCCCCTCGA ACGCTTTCTA CAAGATCCAC TGCAAATCAG 
TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAACTA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_011526137.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524439.1 Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

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CloneID	OHu60286
Clone ID Related Accession (Same CDS sequence)	XM_011526137.3 , XM_011526137.2
Accession Version	XM_011526137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011526137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAT TGGATGAAGA GGGAAATCAA AGAAGAAATC TAGAATCTAC AGTGTCTCAG 
ATTGAGAAGG AGAAAATGTT GCTACAGCAT AGAATTAATG AGTACCAAAG AAAAGCTGAA 
CAGGAAAATG AGAAGAGAAG AAATGTAGAA AATGAAGTTT CTACATTAAA GGATCAGTTG 
GAAGACTTAA AGAAAGTCAG TCAGAATTCA CAGCTTGCTA ATGAGAAGCT GTCCCAGTTA 
CAAAAGCAGC TAGAAGAAGC CAATGACTTA CTTAGGACAG AATCGGACAC AGCTGTAAGA 
TTGAGGAAGA GTCACACAGA GATGAGCAAG TCAATTAGTC AGTTAGAGTC CCTGAACAGA 
GAGTTGCAAG AGAGAAATCG AATTTTAGAG AATTCTAAGT CACAAACAGA CAAAGATTAT 
TACCAGCTGC AAGCTATATT AGAAGCTGAA CGAAGAGACA GAGGTCATGA TTCTGAGATG 
ATTGGAGACC TTCAAGCTCG AATTACATCT TTACAAGAGG AGGTGAAGCA TCTCAAACAT 
AATCTCGAAA AAGTGGAAGG AGAAAGAAAA GAGGCTCAAG ACATGCTTAA TCACTCAGAA 
AAGGAAAAGA ATAATTTAGA GATAGATTTA AACTACAAAC TTAAATCATT ACAACAACGG 
TTAGAACAAG AGGTAAATGA ACACAAAGTA ACCAAAGCTC GTTTAACTGA CAAACATCAA 
TCTATTGAAG AGGCAAAGTC TGTGGCAATG TGTGAGATGG AAAAAAAGCT GAAAGAAGAA 
AGAGAAGCTC GAGAGAAGGC TGAAAATCGG GTTGTTCAGA TTGAGAAACA GTGTTCCATG 
CTAGACGTTG ATCTGAAGCA ATCTCAGCAG AAACTAGAAC ATTTGACTGG AAATAAAGAA 
AGGATGGAGG ATGAAGTTAA GAATCTAACC CTGCAACTGG AGCAGGAATC AAATAAGCGG 
CTGTTGTTAC AAAATGAATT GAAGACTCAA GCATTTGAGG CAGACAATTT AAAAGGTTTA 
GAAAAGCAGA TGAAACAGGA AATAAATACT TTATTGGAAG CAAAGAGATT ATTAGAATTT 
GAGTTAGCTC AGCTTACGAA ACAGTATAGA GGAAATGAAG GACAGATGCG GGAGCTACAA 
GATCAGCTTG AAGCTGAGCA ATATTTCTCG ACACTTTATA AAACCCAGGT AAAGGAACTT 
AAAGAAGAAA TTGAAGAAAA AAACAGAGAA AATTTAAAGA AAATACAGGA ACTACAAAAT 
GAAAAAGAAA CTCTTGCTAC TCAGTTGGAT CTAGCAGAAA CAAAAGCTGA GTCTGAGCAG 
TTGGCGCGAG GCCTTCTGGA AGAACAGTAT TTTGAATTGA CGCAAGAAAG CAAGAAAGCT 
GCTTCAAGAA ATAGACAAGA GATTACAGAT AAAGATCACA CTGTTAGTCG GCTTGAAGAA 
GCAAACAGCA TGCTAACCAA AGATATTGAA ATATTAAGAA GAGAGAATGA AGAGCTAACA 
GAGAAAATGA AGAAGGCAGA GGAAGAATAT AAACTGGAGA AGGAGGAGGA GATCAGTAAT 
CTTAAGGCTG CCTTTGAAAA GAATATCAAC ACTGAACGAA CCCTTAAAAC ACAGGCTGTT 
AACAAATTGG CAGAAATAAT GAATCGAAAA GATTTTAAAA TTGATAGAAA GAAAGCTAAT 
ACACAAGATT TGAGAAAGAA AGAAAAGGAA AATCGAAAGC TGCAACTGGA ACTCAACCAA 
GAAAGAGAGA AATTCAACCA GATGGTAGTG AAACATCAGA AGGAACTGAA TGACATGCAA 
GCGCAATTGG TAGAAGAATG TGCACATAGG AATGAGCTTC AGATGCAGTT GGCCAGCAAA 
GAGAGTGATA TTGAGCAATT GCGTGCTAAA CTTTTGGACC TCTCGGATTC TACAAGTGTT 
GCTAGTTTTC CTAGTGCTGA TGAAACTGAT GGTAACCTCC CAGAGTCAAG AATTGAAGGT 
TGGCTTTCAG TACCAAATAG AGGAAATATC AAACGATATG GCTGGAAGAA ACAGTATGTT 
GTGGTAAGCA GCAAAAAAAT TTTGTTCTAT AATGACGAAC AAGATAAGGA GCAATCCAAT 
CCATCTATGG TATTGGACAT AGATAAACTG TTTCACGTTA GACCTGTAAC CCAAGGAGAT 
GTGTATAGAG CTGAAACTGA AGAAATTCCT AAAATATTCC AGATACTATA TGCAAATGAA 
GGTGAATGTA GAAAAGATGT AGAGATGGAA CCAGTACAAC AAGCTGAAAA AACTAATTTC 
CAAAATCACA AAGGCCATGA GTTTATTCCT ACACTCTACC ACTTTCCTGC CAATTGTGAT 
GCCTGTGCCA AACCTCTCTG GCATGTTTTT AAGCCACCCC CTGCCCTAGA GTGTCGAAGA 
TGCCATGTTA AGTGCCACAG AGATCACTTA GATAAGAAAG AGGACTTAAT TTGTCCATGT 
AAAGTAAGTT ATGATGTAAC ATCAGCAAGA GATATGCTGC TGTTAGCATG TTCTCAGGAT 
GAACAAAAAA AATGGGTAAC TCATTTAGTA AAGAAAATCC CTAAGAATCC ACCATCTGGT 
TTTGTTCGTG CTTCCCCTCG AACGCTTTCT ACAAGATCCA CTGCAAATCA GTCTTTCCGG 
AAAGTGGTCA AAAATACATC TGGAAAAACT AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524439.1
CDS	565..3240
Translation	MKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	XM_011526137.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526137.3

ORF Insert Sequence:

ATGAAAGAAT TGGATGAAGA GGGAAATCAA AGAAGAAATC TAGAATCTAC AGTGTCTCAG 
ATTGAGAAGG AGAAAATGTT GCTACAGCAT AGAATTAATG AGTACCAAAG AAAAGCTGAA 
CAGGAAAATG AGAAGAGAAG AAATGTAGAA AATGAAGTTT CTACATTAAA GGATCAGTTG 
GAAGACTTAA AGAAAGTCAG TCAGAATTCA CAGCTTGCTA ATGAGAAGCT GTCCCAGTTA 
CAAAAGCAGC TAGAAGAAGC CAATGACTTA CTTAGGACAG AATCGGACAC AGCTGTAAGA 
TTGAGGAAGA GTCACACAGA GATGAGCAAG TCAATTAGTC AGTTAGAGTC CCTGAACAGA 
GAGTTGCAAG AGAGAAATCG AATTTTAGAG AATTCTAAGT CACAAACAGA CAAAGATTAT 
TACCAGCTGC AAGCTATATT AGAAGCTGAA CGAAGAGACA GAGGTCATGA TTCTGAGATG 
ATTGGAGACC TTCAAGCTCG AATTACATCT TTACAAGAGG AGGTGAAGCA TCTCAAACAT 
AATCTCGAAA AAGTGGAAGG AGAAAGAAAA GAGGCTCAAG ACATGCTTAA TCACTCAGAA 
AAGGAAAAGA ATAATTTAGA GATAGATTTA AACTACAAAC TTAAATCATT ACAACAACGG 
TTAGAACAAG AGGTAAATGA ACACAAAGTA ACCAAAGCTC GTTTAACTGA CAAACATCAA 
TCTATTGAAG AGGCAAAGTC TGTGGCAATG TGTGAGATGG AAAAAAAGCT GAAAGAAGAA 
AGAGAAGCTC GAGAGAAGGC TGAAAATCGG GTTGTTCAGA TTGAGAAACA GTGTTCCATG 
CTAGACGTTG ATCTGAAGCA ATCTCAGCAG AAACTAGAAC ATTTGACTGG AAATAAAGAA 
AGGATGGAGG ATGAAGTTAA GAATCTAACC CTGCAACTGG AGCAGGAATC AAATAAGCGG 
CTGTTGTTAC AAAATGAATT GAAGACTCAA GCATTTGAGG CAGACAATTT AAAAGGTTTA 
GAAAAGCAGA TGAAACAGGA AATAAATACT TTATTGGAAG CAAAGAGATT ATTAGAATTT 
GAGTTAGCTC AGCTTACGAA ACAGTATAGA GGAAATGAAG GACAGATGCG GGAGCTACAA 
GATCAGCTTG AAGCTGAGCA ATATTTCTCG ACACTTTATA AAACCCAGGT AAAGGAACTT 
AAAGAAGAAA TTGAAGAAAA AAACAGAGAA AATTTAAAGA AAATACAGGA ACTACAAAAT 
GAAAAAGAAA CTCTTGCTAC TCAGTTGGAT CTAGCAGAAA CAAAAGCTGA GTCTGAGCAG 
TTGGCGCGAG GCCTTCTGGA AGAACAGTAT TTTGAATTGA CGCAAGAAAG CAAGAAAGCT 
GCTTCAAGAA ATAGACAAGA GATTACAGAT AAAGATCACA CTGTTAGTCG GCTTGAAGAA 
GCAAACAGCA TGCTAACCAA AGATATTGAA ATATTAAGAA GAGAGAATGA AGAGCTAACA 
GAGAAAATGA AGAAGGCAGA GGAAGAATAT AAACTGGAGA AGGAGGAGGA GATCAGTAAT 
CTTAAGGCTG CCTTTGAAAA GAATATCAAC ACTGAACGAA CCCTTAAAAC ACAGGCTGTT 
AACAAATTGG CAGAAATAAT GAATCGAAAA GATTTTAAAA TTGATAGAAA GAAAGCTAAT 
ACACAAGATT TGAGAAAGAA AGAAAAGGAA AATCGAAAGC TGCAACTGGA ACTCAACCAA 
GAAAGAGAGA AATTCAACCA GATGGTAGTG AAACATCAGA AGGAACTGAA TGACATGCAA 
GCGCAATTGG TAGAAGAATG TGCACATAGG AATGAGCTTC AGATGCAGTT GGCCAGCAAA 
GAGAGTGATA TTGAGCAATT GCGTGCTAAA CTTTTGGACC TCTCGGATTC TACAAGTGTT 
GCTAGTTTTC CTAGTGCTGA TGAAACTGAT GGTAACCTCC CAGAGTCAAG AATTGAAGGT 
TGGCTTTCAG TACCAAATAG AGGAAATATC AAACGATATG GCTGGAAGAA ACAGTATGTT 
GTGGTAAGCA GCAAAAAAAT TTTGTTCTAT AATGACGAAC AAGATAAGGA GCAATCCAAT 
CCATCTATGG TATTGGACAT AGATAAACTG TTTCACGTTA GACCTGTAAC CCAAGGAGAT 
GTGTATAGAG CTGAAACTGA AGAAATTCCT AAAATATTCC AGATACTATA TGCAAATGAA 
GGTGAATGTA GAAAAGATGT AGAGATGGAA CCAGTACAAC AAGCTGAAAA AACTAATTTC 
CAAAATCACA AAGGCCATGA GTTTATTCCT ACACTCTACC ACTTTCCTGC CAATTGTGAT 
GCCTGTGCCA AACCTCTCTG GCATGTTTTT AAGCCACCCC CTGCCCTAGA GTGTCGAAGA 
TGCCATGTTA AGTGCCACAG AGATCACTTA GATAAGAAAG AGGACTTAAT TTGTCCATGT 
AAAGTAAGTT ATGATGTAAC ATCAGCAAGA GATATGCTGC TGTTAGCATG TTCTCAGGAT 
GAACAAAAAA AATGGGTAAC TCATTTAGTA AAGAAAATCC CTAAGAATCC ACCATCTGGT 
TTTGTTCGTG CTTCCCCTCG AACGCTTTCT ACAAGATCCA CTGCAAATCA GTCTTTCCGG 
AAAGTGGTCA AAAATACATC TGGAAAAACT AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK1 ( XM_011526137.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524439.1 Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

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CloneID	OHu60286
Clone ID Related Accession (Same CDS sequence)	XM_011526137.3 , XM_011526137.2
Accession Version	XM_011526137.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAT TGGATGAAGA GGGAAATCAA AGAAGAAATC TAGAATCTAC AGTGTCTCAG 
ATTGAGAAGG AGAAAATGTT GCTACAGCAT AGAATTAATG AGTACCAAAG AAAAGCTGAA 
CAGGAAAATG AGAAGAGAAG AAATGTAGAA AATGAAGTTT CTACATTAAA GGATCAGTTG 
GAAGACTTAA AGAAAGTCAG TCAGAATTCA CAGCTTGCTA ATGAGAAGCT GTCCCAGTTA 
CAAAAGCAGC TAGAAGAAGC CAATGACTTA CTTAGGACAG AATCGGACAC AGCTGTAAGA 
TTGAGGAAGA GTCACACAGA GATGAGCAAG TCAATTAGTC AGTTAGAGTC CCTGAACAGA 
GAGTTGCAAG AGAGAAATCG AATTTTAGAG AATTCTAAGT CACAAACAGA CAAAGATTAT 
TACCAGCTGC AAGCTATATT AGAAGCTGAA CGAAGAGACA GAGGTCATGA TTCTGAGATG 
ATTGGAGACC TTCAAGCTCG AATTACATCT TTACAAGAGG AGGTGAAGCA TCTCAAACAT 
AATCTCGAAA AAGTGGAAGG AGAAAGAAAA GAGGCTCAAG ACATGCTTAA TCACTCAGAA 
AAGGAAAAGA ATAATTTAGA GATAGATTTA AACTACAAAC TTAAATCATT ACAACAACGG 
TTAGAACAAG AGGTAAATGA ACACAAAGTA ACCAAAGCTC GTTTAACTGA CAAACATCAA 
TCTATTGAAG AGGCAAAGTC TGTGGCAATG TGTGAGATGG AAAAAAAGCT GAAAGAAGAA 
AGAGAAGCTC GAGAGAAGGC TGAAAATCGG GTTGTTCAGA TTGAGAAACA GTGTTCCATG 
CTAGACGTTG ATCTGAAGCA ATCTCAGCAG AAACTAGAAC ATTTGACTGG AAATAAAGAA 
AGGATGGAGG ATGAAGTTAA GAATCTAACC CTGCAACTGG AGCAGGAATC AAATAAGCGG 
CTGTTGTTAC AAAATGAATT GAAGACTCAA GCATTTGAGG CAGACAATTT AAAAGGTTTA 
GAAAAGCAGA TGAAACAGGA AATAAATACT TTATTGGAAG CAAAGAGATT ATTAGAATTT 
GAGTTAGCTC AGCTTACGAA ACAGTATAGA GGAAATGAAG GACAGATGCG GGAGCTACAA 
GATCAGCTTG AAGCTGAGCA ATATTTCTCG ACACTTTATA AAACCCAGGT AAAGGAACTT 
AAAGAAGAAA TTGAAGAAAA AAACAGAGAA AATTTAAAGA AAATACAGGA ACTACAAAAT 
GAAAAAGAAA CTCTTGCTAC TCAGTTGGAT CTAGCAGAAA CAAAAGCTGA GTCTGAGCAG 
TTGGCGCGAG GCCTTCTGGA AGAACAGTAT TTTGAATTGA CGCAAGAAAG CAAGAAAGCT 
GCTTCAAGAA ATAGACAAGA GATTACAGAT AAAGATCACA CTGTTAGTCG GCTTGAAGAA 
GCAAACAGCA TGCTAACCAA AGATATTGAA ATATTAAGAA GAGAGAATGA AGAGCTAACA 
GAGAAAATGA AGAAGGCAGA GGAAGAATAT AAACTGGAGA AGGAGGAGGA GATCAGTAAT 
CTTAAGGCTG CCTTTGAAAA GAATATCAAC ACTGAACGAA CCCTTAAAAC ACAGGCTGTT 
AACAAATTGG CAGAAATAAT GAATCGAAAA GATTTTAAAA TTGATAGAAA GAAAGCTAAT 
ACACAAGATT TGAGAAAGAA AGAAAAGGAA AATCGAAAGC TGCAACTGGA ACTCAACCAA 
GAAAGAGAGA AATTCAACCA GATGGTAGTG AAACATCAGA AGGAACTGAA TGACATGCAA 
GCGCAATTGG TAGAAGAATG TGCACATAGG AATGAGCTTC AGATGCAGTT GGCCAGCAAA 
GAGAGTGATA TTGAGCAATT GCGTGCTAAA CTTTTGGACC TCTCGGATTC TACAAGTGTT 
GCTAGTTTTC CTAGTGCTGA TGAAACTGAT GGTAACCTCC CAGAGTCAAG AATTGAAGGT 
TGGCTTTCAG TACCAAATAG AGGAAATATC AAACGATATG GCTGGAAGAA ACAGTATGTT 
GTGGTAAGCA GCAAAAAAAT TTTGTTCTAT AATGACGAAC AAGATAAGGA GCAATCCAAT 
CCATCTATGG TATTGGACAT AGATAAACTG TTTCACGTTA GACCTGTAAC CCAAGGAGAT 
GTGTATAGAG CTGAAACTGA AGAAATTCCT AAAATATTCC AGATACTATA TGCAAATGAA 
GGTGAATGTA GAAAAGATGT AGAGATGGAA CCAGTACAAC AAGCTGAAAA AACTAATTTC 
CAAAATCACA AAGGCCATGA GTTTATTCCT ACACTCTACC ACTTTCCTGC CAATTGTGAT 
GCCTGTGCCA AACCTCTCTG GCATGTTTTT AAGCCACCCC CTGCCCTAGA GTGTCGAAGA 
TGCCATGTTA AGTGCCACAG AGATCACTTA GATAAGAAAG AGGACTTAAT TTGTCCATGT 
AAAGTAAGTT ATGATGTAAC ATCAGCAAGA GATATGCTGC TGTTAGCATG TTCTCAGGAT 
GAACAAAAAA AATGGGTAAC TCATTTAGTA AAGAAAATCC CTAAGAATCC ACCATCTGGT 
TTTGTTCGTG CTTCCCCTCG AACGCTTTCT ACAAGATCCA CTGCAAATCA GTCTTTCCGG 
AAAGTGGTCA AAAATACATC TGGAAAAACT AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524439.1
CDS	565..3240
Translation	MKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS

Target ORF information:

RefSeq Version	XM_011526137.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526137.2

ORF Insert Sequence:

ATGAAAGAAT TGGATGAAGA GGGAAATCAA AGAAGAAATC TAGAATCTAC AGTGTCTCAG 
ATTGAGAAGG AGAAAATGTT GCTACAGCAT AGAATTAATG AGTACCAAAG AAAAGCTGAA 
CAGGAAAATG AGAAGAGAAG AAATGTAGAA AATGAAGTTT CTACATTAAA GGATCAGTTG 
GAAGACTTAA AGAAAGTCAG TCAGAATTCA CAGCTTGCTA ATGAGAAGCT GTCCCAGTTA 
CAAAAGCAGC TAGAAGAAGC CAATGACTTA CTTAGGACAG AATCGGACAC AGCTGTAAGA 
TTGAGGAAGA GTCACACAGA GATGAGCAAG TCAATTAGTC AGTTAGAGTC CCTGAACAGA 
GAGTTGCAAG AGAGAAATCG AATTTTAGAG AATTCTAAGT CACAAACAGA CAAAGATTAT 
TACCAGCTGC AAGCTATATT AGAAGCTGAA CGAAGAGACA GAGGTCATGA TTCTGAGATG 
ATTGGAGACC TTCAAGCTCG AATTACATCT TTACAAGAGG AGGTGAAGCA TCTCAAACAT 
AATCTCGAAA AAGTGGAAGG AGAAAGAAAA GAGGCTCAAG ACATGCTTAA TCACTCAGAA 
AAGGAAAAGA ATAATTTAGA GATAGATTTA AACTACAAAC TTAAATCATT ACAACAACGG 
TTAGAACAAG AGGTAAATGA ACACAAAGTA ACCAAAGCTC GTTTAACTGA CAAACATCAA 
TCTATTGAAG AGGCAAAGTC TGTGGCAATG TGTGAGATGG AAAAAAAGCT GAAAGAAGAA 
AGAGAAGCTC GAGAGAAGGC TGAAAATCGG GTTGTTCAGA TTGAGAAACA GTGTTCCATG 
CTAGACGTTG ATCTGAAGCA ATCTCAGCAG AAACTAGAAC ATTTGACTGG AAATAAAGAA 
AGGATGGAGG ATGAAGTTAA GAATCTAACC CTGCAACTGG AGCAGGAATC AAATAAGCGG 
CTGTTGTTAC AAAATGAATT GAAGACTCAA GCATTTGAGG CAGACAATTT AAAAGGTTTA 
GAAAAGCAGA TGAAACAGGA AATAAATACT TTATTGGAAG CAAAGAGATT ATTAGAATTT 
GAGTTAGCTC AGCTTACGAA ACAGTATAGA GGAAATGAAG GACAGATGCG GGAGCTACAA 
GATCAGCTTG AAGCTGAGCA ATATTTCTCG ACACTTTATA AAACCCAGGT AAAGGAACTT 
AAAGAAGAAA TTGAAGAAAA AAACAGAGAA AATTTAAAGA AAATACAGGA ACTACAAAAT 
GAAAAAGAAA CTCTTGCTAC TCAGTTGGAT CTAGCAGAAA CAAAAGCTGA GTCTGAGCAG 
TTGGCGCGAG GCCTTCTGGA AGAACAGTAT TTTGAATTGA CGCAAGAAAG CAAGAAAGCT 
GCTTCAAGAA ATAGACAAGA GATTACAGAT AAAGATCACA CTGTTAGTCG GCTTGAAGAA 
GCAAACAGCA TGCTAACCAA AGATATTGAA ATATTAAGAA GAGAGAATGA AGAGCTAACA 
GAGAAAATGA AGAAGGCAGA GGAAGAATAT AAACTGGAGA AGGAGGAGGA GATCAGTAAT 
CTTAAGGCTG CCTTTGAAAA GAATATCAAC ACTGAACGAA CCCTTAAAAC ACAGGCTGTT 
AACAAATTGG CAGAAATAAT GAATCGAAAA GATTTTAAAA TTGATAGAAA GAAAGCTAAT 
ACACAAGATT TGAGAAAGAA AGAAAAGGAA AATCGAAAGC TGCAACTGGA ACTCAACCAA 
GAAAGAGAGA AATTCAACCA GATGGTAGTG AAACATCAGA AGGAACTGAA TGACATGCAA 
GCGCAATTGG TAGAAGAATG TGCACATAGG AATGAGCTTC AGATGCAGTT GGCCAGCAAA 
GAGAGTGATA TTGAGCAATT GCGTGCTAAA CTTTTGGACC TCTCGGATTC TACAAGTGTT 
GCTAGTTTTC CTAGTGCTGA TGAAACTGAT GGTAACCTCC CAGAGTCAAG AATTGAAGGT 
TGGCTTTCAG TACCAAATAG AGGAAATATC AAACGATATG GCTGGAAGAA ACAGTATGTT 
GTGGTAAGCA GCAAAAAAAT TTTGTTCTAT AATGACGAAC AAGATAAGGA GCAATCCAAT 
CCATCTATGG TATTGGACAT AGATAAACTG TTTCACGTTA GACCTGTAAC CCAAGGAGAT 
GTGTATAGAG CTGAAACTGA AGAAATTCCT AAAATATTCC AGATACTATA TGCAAATGAA 
GGTGAATGTA GAAAAGATGT AGAGATGGAA CCAGTACAAC AAGCTGAAAA AACTAATTTC 
CAAAATCACA AAGGCCATGA GTTTATTCCT ACACTCTACC ACTTTCCTGC CAATTGTGAT 
GCCTGTGCCA AACCTCTCTG GCATGTTTTT AAGCCACCCC CTGCCCTAGA GTGTCGAAGA 
TGCCATGTTA AGTGCCACAG AGATCACTTA GATAAGAAAG AGGACTTAAT TTGTCCATGT 
AAAGTAAGTT ATGATGTAAC ATCAGCAAGA GATATGCTGC TGTTAGCATG TTCTCAGGAT 
GAACAAAAAA AATGGGTAAC TCATTTAGTA AAGAAAATCC CTAAGAATCC ACCATCTGGT 
TTTGTTCGTG CTTCCCCTCG AACGCTTTCT ACAAGATCCA CTGCAAATCA GTCTTTCCGG 
AAAGTGGTCA AAAATACATC TGGAAAAACT AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ROCK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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