MRPS35 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPS35
Entrez Gene ID	60488
Full Name	mitochondrial ribosomal protein S35
Synonyms	HDCMD11P,MDS023,MRP-S28,MRPS28
General protein information	Preferred Names mitochondrial ribosomal protein S35 Names 28S ribosomal protein S35, mitochondrial 28S ribosomal protein S28, mitochondrial MRP-S35 S28mt S35mt mitochondrial ribosomal protein S28 mitochondrial small ribosomal subunit protein mS35
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p11.22
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010].
Disorder MIM:	611995

mRNA and Protein(s)

mRNA	Protein	Name
NM_021821.3	NP_068593.2	28S ribosomal protein S35, mitochondrial isoform 1 precursor
XM_017019780.1	XP_016875269.1	28S ribosomal protein S35, mitochondrial isoform X1
NM_001190864.1	NP_001177793.1	28S ribosomal protein S35, mitochondrial isoform 2 precursor
NM_021821.4	NP_068593.2	28S ribosomal protein S35, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mrps35	NP_001100098.1
Gallus gallus (chicken)	MRPS35	NP_001265020.1
Danio rerio (zebrafish)	mrps35	NP_001026839.1
Drosophila melanogaster (fruit fly)	mRpS35	NP_523893.1
Canis lupus familiaris (dog)	MRPS35	XP_005637084.1
Caenorhabditis elegans (roundworm)	mrps-35	NP_001256837.1
Pan troglodytes (chimpanzee)	MRPS35	XP_003313819.2
	MRPS35	XP_001103832.1
Mus musculus (house mouse)	Mrps35	NP_663548.2
Homo sapiens (human)	MRPS35	NP_068593.2
Bos taurus (cattle)	MRPS35	NP_001069087.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006802	XP_308945.4
Xenopus tropicalis (tropical clawed frog)	mrps35	NP_001016014.1

Gene Ontology
Bibliography

Related articles in PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

ZNF143 activates gene expression in response to DNA damage and binds to cisplatin-modified DNA.
Ishiguchi H, Izumi H, Torigoe T, Yoshida Y, Kubota H, Tsuji S, Kohno K
International journal of cancer111(6)900-9(2004 Oct)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach.
Koc EC, Burkhart W, Blackburn K, Koc H, Moseley A, Spremulli LL
Protein science : a publication of the Protein Society10(3)471-81(2001 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55802	NM_021821.4 Latest version!	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu09333	NM_001190864.1 Latest version!	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu84046	XM_017019780.1 Latest version!	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu55802	NM_021821.3	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPS35 ( NM_021821.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068593.2 Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55802
Clone ID Related Accession (Same CDS sequence)	NM_021821.3 , NM_021821.4
Accession Version	NM_021821.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	28S ribosomal protein S35, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA747094.1, BC015862.1 and AI538796.1. On Jul 2, 2010 this sequence version replaced NM_021821.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.75178.1, SRR1803616.248451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGTAAAGCT TTCCAGTTTG 
AATTTAGATG ATCACGCAAA GAAGAAATTA ATTAAACTTG TAGGAGAGCG ATACTGCAAG 
ACCACAGATG TGCTTACCAT CAAAACAGAT AGGTGCCCTT TAAGGAGGCA GAATTACGAT 
TATGCAGTGT ATCTACTAAC AGTGTTATAC CATGAGTCTT GGAATACTGA AGAATGGGAA 
AAAAGTAAGA CTGAAGCAGA CATGGAAGAG TATATATGGG AAAATAGCTC ATCAGAAAGA 
AATATCCTGG AAACGCTTCT CCAGATGAAA GCTGCTGAGA AAAATATGGA AATAAATAAA 
GAAGAGCTCC TTGGTACTAA AGAAATTGAA GAGTACAAAA AGTCTGTTGT TAGTCTTAAA 
AATGAGGAGG AAAATGAAAA TTCCATTTCT CAGTACAAAG AATCCGTGAA GAGACTATTA 
AATGTGACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068593.2
CDS	72..1043
Translation	MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLRVKLSSLNLDDHAKKKLIKLVGERYCKTTDVLTIKTDRCPLRRQNYDYAVYLLTVLYHESWNTEEWEKSKTEADMEEYIWENSSSERNILETLLQMKAAEKNMEINKEELLGTKEIEEYKKSVVSLKNEEENENSISQYKESVKRLLNVT

Target ORF information:

RefSeq Version	NM_021821.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021821.3

ORF Insert Sequence:

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGTAAAGCT TTCCAGTTTG 
AATTTAGATG ATCACGCAAA GAAGAAATTA ATTAAACTTG TAGGAGAGCG ATACTGCAAG 
ACCACAGATG TGCTTACCAT CAAAACAGAT AGGTGCCCTT TAAGGAGGCA GAATTACGAT 
TATGCAGTGT ATCTACTAAC AGTGTTATAC CATGAGTCTT GGAATACTGA AGAATGGGAA 
AAAAGTAAGA CTGAAGCAGA CATGGAAGAG TATATATGGG AAAATAGCTC ATCAGAAAGA 
AATATCCTGG AAACGCTTCT CCAGATGAAA GCTGCTGAGA AAAATATGGA AATAAATAAA 
GAAGAGCTCC TTGGTACTAA AGAAATTGAA GAGTACAAAA AGTCTGTTGT TAGTCTTAAA 
AATGAGGAGG AAAATGAAAA TTCCATTTCT CAGTACAAAG AATCCGTGAA GAGACTATTA 
AATGTGACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS35 ( NM_021821.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068593.2 Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55802
Clone ID Related Accession (Same CDS sequence)	NM_021821.3 , NM_021821.4
Accession Version	NM_021821.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	28S ribosomal protein S35, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA747094.1 and BC015862.1. On Nov 23, 2018 this sequence version replaced NM_021821.3. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC015862.1, SRR3476690.366597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000081029.8/ ENSP00000081029.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGTAAAGCT TTCCAGTTTG 
AATTTAGATG ATCACGCAAA GAAGAAATTA ATTAAACTTG TAGGAGAGCG ATACTGCAAG 
ACCACAGATG TGCTTACCAT CAAAACAGAT AGGTGCCCTT TAAGGAGGCA GAATTACGAT 
TATGCAGTGT ATCTACTAAC AGTGTTATAC CATGAGTCTT GGAATACTGA AGAATGGGAA 
AAAAGTAAGA CTGAAGCAGA CATGGAAGAG TATATATGGG AAAATAGCTC ATCAGAAAGA 
AATATCCTGG AAACGCTTCT CCAGATGAAA GCTGCTGAGA AAAATATGGA AATAAATAAA 
GAAGAGCTCC TTGGTACTAA AGAAATTGAA GAGTACAAAA AGTCTGTTGT TAGTCTTAAA 
AATGAGGAGG AAAATGAAAA TTCCATTTCT CAGTACAAAG AATCCGTGAA GAGACTATTA 
AATGTGACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068593.2
CDS	13..984
Translation	MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLRVKLSSLNLDDHAKKKLIKLVGERYCKTTDVLTIKTDRCPLRRQNYDYAVYLLTVLYHESWNTEEWEKSKTEADMEEYIWENSSSERNILETLLQMKAAEKNMEINKEELLGTKEIEEYKKSVVSLKNEEENENSISQYKESVKRLLNVT

Target ORF information:

RefSeq Version	NM_021821.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021821.4

ORF Insert Sequence:

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGTAAAGCT TTCCAGTTTG 
AATTTAGATG ATCACGCAAA GAAGAAATTA ATTAAACTTG TAGGAGAGCG ATACTGCAAG 
ACCACAGATG TGCTTACCAT CAAAACAGAT AGGTGCCCTT TAAGGAGGCA GAATTACGAT 
TATGCAGTGT ATCTACTAAC AGTGTTATAC CATGAGTCTT GGAATACTGA AGAATGGGAA 
AAAAGTAAGA CTGAAGCAGA CATGGAAGAG TATATATGGG AAAATAGCTC ATCAGAAAGA 
AATATCCTGG AAACGCTTCT CCAGATGAAA GCTGCTGAGA AAAATATGGA AATAAATAAA 
GAAGAGCTCC TTGGTACTAA AGAAATTGAA GAGTACAAAA AGTCTGTTGT TAGTCTTAAA 
AATGAGGAGG AAAATGAAAA TTCCATTTCT CAGTACAAAG AATCCGTGAA GAGACTATTA 
AATGTGACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS35 ( NM_001190864.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177793.1 Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09333
Clone ID Related Accession (Same CDS sequence)	NM_001190864.1
Accession Version	NM_001190864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	28S ribosomal protein S35, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA747094.1, AK075378.1, BC015862.1 and AI538796.1. Transcript Variant: This variant (2) is missing an internal coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.30644.1, AK075378.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGTGCCCTT TAAGGAGGCA 
GAATTACGAT TATGCAGTGT ATCTACTAAC AGTGTTATAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177793.1
CDS	72..656
Translation	MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLRVPFKEAELRLCSVSTNSVIP

Target ORF information:

RefSeq Version	NM_001190864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190864.1

ORF Insert Sequence:

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGTGCCCTT TAAGGAGGCA 
GAATTACGAT TATGCAGTGT ATCTACTAAC AGTGTTATAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS35 ( XM_017019780.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875269.1 Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu84046
Clone ID Related Accession (Same CDS sequence)	XM_017019780.1
Accession Version	XM_017019780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	28S ribosomal protein S35, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGAAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875269.1
CDS	72..602
Translation	MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLREM

Target ORF information:

RefSeq Version	XM_017019780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S35 (MRPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019780.1

ORF Insert Sequence:

ATGGCGGCCG CCGCGCTCCC AGCATGGCTG TCTCTGCAGT CGAGGGCAAG GACTCTGCGT 
GCATTCTCCA CTGCCGTCTA CTCGGCCACT CCGGTCCCGA CACCTAGCCT GCCGGAAAGA 
ACACCCGGAA ATGAAAGGCC ACCAAGAAGA AAGGCACTAC CTCCTAGGAC AGAGAAAATG 
GCTGTTGACC AGGACTGGCC TAGTGTTTAC CCAGTTGCAG CACCATTTAA ACCCTCTGCA 
GTACCTCTTC CTGTTCGAAT GGGTTATCCA GTAAAAAAGG GCGTGCCCAT GGCAAAGGAG 
GGAAATCTAG AACTTTTAAA GATTCCCAAT TTTCTGCATT TGACTCCTGT AGCAATTAAA 
AAGCACTGTG AAGCCCTTAA AGATTTTTGC ACTGAGTGGC CAGCCGCACT GGACAGTGAC 
GAGAAATGTG AGAAGCATTT TCCAATTGAA ATTGACAGCA CTGATTATGT TTCATCAGGA 
CCATCTGTTC GGAACCCCAG AGCACGAGTA GTAGTCTTAA GAGAAATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

ZNF143 activates gene expression in response to DNA damage and binds to cisplatin-modified DNA.
International journal of cancer111(6)900-9(2004 Oct)
Ishiguchi H,Izumi H,Torigoe T,Yoshida Y,Kubota H,Tsuji S,Kohno K

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach.
Protein science : a publication of the Protein Society10(3)471-81(2001 Mar)
Koc EC,Burkhart W,Blackburn K,Koc H,Moseley A,Spremulli LL

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MRPS35 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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