RIT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RIT1
Entrez Gene ID	6016
Full Name	Ras like without CAAX 1
Synonyms	NS8,RIBB,RIT,ROC1
General protein information	Preferred Names Ras like without CAAX 1 Names GTP-binding protein Rit1 GTP-binding protein Roc1 Ric-like, expressed in many tissues ras-like protein expressed in many tissues ras-like without CAAX protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q22
Summary	This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012].
Disorder MIM:	609591
Disorder Html:	Noonan syndrome 8, 615355 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006912.5	NP_008843.1	GTP-binding protein Rit1 isoform 2
NM_001256821.1	NP_001243750.1	GTP-binding protein Rit1 isoform 1
NM_006912.6	NP_008843.1	GTP-binding protein Rit1 isoform 2
NM_001256820.1	NP_001243749.1	GTP-binding protein Rit1 isoform 3
NM_001256820.2	NP_001243749.1	GTP-binding protein Rit1 isoform 3
NM_001256821.2	NP_001243750.1	GTP-binding protein Rit1 isoform 1

Pathways from BioSystems

Homologies

	RIT1	XP_001116270.1
Bos taurus (cattle)	RIT1	NP_001091547.1
Pan troglodytes (chimpanzee)	RIT1	XP_513868.4
Canis lupus familiaris (dog)	RIT1	XP_537249.2
Mus musculus (house mouse)	Rit1	NP_033095.1
Gallus gallus (chicken)	RIT1	NP_001026498.2
Danio rerio (zebrafish)	rit1	NP_001122253.1
Xenopus tropicalis (tropical clawed frog)	LOC100486360	XP_002944039.2
Homo sapiens (human)	RIT1	NP_001243750.1
Rattus norvegicus (Norway rat)	Rit1	NP_001102655.1

Gene Ontology
Bibliography

Related articles in PubMed

Expanding the cardiac spectrum of Noonan syndrome with RIT1 variant: Left main coronary artery atresia causing sudden death.
Ramond F, Duband S, Croisille P, Cav? H, Teyssier G, Adouard V, Touraine R
European journal of medical genetics60(6)299-302(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Congenital left main coronary artery atresia in a Noonan syndrome is associated with RIT1 variant, leading to unrescued sudden death.
Title: Expanding the cardiac spectrum of Noonan syndrome with RIT1 variant: Left main coronary artery atresia causing sudden death.

RIT1 is one of the major genes for NS. The RIT1-associated phenotype differs gradually from other NS subtypes, with a high prevalence of cardiovascular manifestations, especially hypertrophic cardiomyopathy.
Title: Genotype and phenotype in patients with Noonan syndrome and a RIT1 mutation.

Noonan patients with germline RIT1 mutations are not at high risk to developing JMML or ALL, and that RIT1 has at most a marginal role in these sporadic malignancies.
Title: Mutations in RIT1 cause Noonan syndrome with possible juvenile myelomonocytic leukemia but are not involved in acute lymphoblastic leukemia.

Our report contributes to the delineation of the phenotype associated with RIT1 mutations and underlines that lymphatic involvement is part of this spectrum
Title: Two cases of RIT1 associated Noonan syndrome: Further delineation of the clinical phenotype and review of the literature.

Biochemical Classification of Disease-associated Mutants of RAS-like Protein Expressed in Many Tissues (RIT1).
Title: Biochemical Classification of Disease-associated Mutants of RAS-like Protein Expressed in Many Tissues (RIT1).

The following RIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12934	NM_001256820.2 Latest version!	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22544	NM_006912.6 Latest version!	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu12558	NM_001256821.2 Latest version!	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu22544	NM_006912.5	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu12934	NM_001256820.1	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu12558	NM_001256821.1	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIT1 ( NM_001256820.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243749.1 Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12934
Clone ID Related Accession (Same CDS sequence)	NM_001256820.1 , NM_001256820.2
Accession Version	NM_001256820.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	GTP-binding protein Rit1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298768.1, AL139128.24 and BU674989.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences. These difference cause translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298768.1, SRR1803613.253021.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGACCATGC AGTTCATCAG CCACCGATTC CCAGAAGATC ATGATCCCAC CATTGAAGAT 
GCTTATAAGA TCAGGATCCG TATTGATGAT GAGCCTGCCA ATCTGGACAT TTTGGATACA 
GCTGGACAGG CAGAGTTTAC AGCCATGCGG GACCAGTATA TGAGGGCAGG AGAAGGGTTT 
ATCATCTGTT ACTCTATCAC GGATCGTCGA AGTTTCCATG AAGTTCGTGA GTTTAAACAG 
CTTATTTATC GAGTCCGACG TACTGACGAT ACACCTGTGG TTCTTGTGGG AAACAAGTCA 
GACCTCAAAC AGCTAAGACA GGTCACCAAG GAAGAAGGAT TGGCCTTGGC CCGAGAATTC 
AGCTGTCCCT TTTTTGAGAC ATCTGCTGCA TACCGCTACT ATATTGATGA TGTTTTCCAT 
GCCCTTGTAC GGGAGATACG TAGGAAAGAA AAGGAGGCAG TACTGGCCAT GGAGAAAAAA 
TCTAAGCCCA AAAACAGTGT ATGGAAGAGG CTAAAATCAC CATTCCGGAA GAAGAAAGAT 
TCAGTAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243749.1
CDS	163..714
Translation	MTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

Target ORF information:

RefSeq Version	NM_001256820.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256820.1

ORF Insert Sequence:

ATGACCATGC AGTTCATCAG CCACCGATTC CCAGAAGATC ATGATCCCAC CATTGAAGAT 
GCTTATAAGA TCAGGATCCG TATTGATGAT GAGCCTGCCA ATCTGGACAT TTTGGATACA 
GCTGGACAGG CAGAGTTTAC AGCCATGCGG GACCAGTATA TGAGGGCAGG AGAAGGGTTT 
ATCATCTGTT ACTCTATCAC GGATCGTCGA AGTTTCCATG AAGTTCGTGA GTTTAAACAG 
CTTATTTATC GAGTCCGACG TACTGACGAT ACACCTGTGG TTCTTGTGGG AAACAAGTCA 
GACCTCAAAC AGCTAAGACA GGTCACCAAG GAAGAAGGAT TGGCCTTGGC CCGAGAATTC 
AGCTGTCCCT TTTTTGAGAC ATCTGCTGCA TACCGCTACT ATATTGATGA TGTTTTCCAT 
GCCCTTGTAC GGGAGATACG TAGGAAAGAA AAGGAGGCAG TACTGGCCAT GGAGAAAAAA 
TCTAAGCCCA AAAACAGTGT ATGGAAGAGG CTAAAATCAC CATTCCGGAA GAAGAAAGAT 
TCAGTAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIT1 ( NM_001256820.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243749.1 Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12934
Clone ID Related Accession (Same CDS sequence)	NM_001256820.1 , NM_001256820.2
Accession Version	NM_001256820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	GTP-binding protein Rit1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298768.1, AL139128.24 and BU674989.1. On May 31, 2019 this sequence version replaced NM_001256820.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences. These difference cause translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.253021.1, AK298768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGACCATGC AGTTCATCAG CCACCGATTC CCAGAAGATC ATGATCCCAC CATTGAAGAT 
GCTTATAAGA TCAGGATCCG TATTGATGAT GAGCCTGCCA ATCTGGACAT TTTGGATACA 
GCTGGACAGG CAGAGTTTAC AGCCATGCGG GACCAGTATA TGAGGGCAGG AGAAGGGTTT 
ATCATCTGTT ACTCTATCAC GGATCGTCGA AGTTTCCATG AAGTTCGTGA GTTTAAACAG 
CTTATTTATC GAGTCCGACG TACTGACGAT ACACCTGTGG TTCTTGTGGG AAACAAGTCA 
GACCTCAAAC AGCTAAGACA GGTCACCAAG GAAGAAGGAT TGGCCTTGGC CCGAGAATTC 
AGCTGTCCCT TTTTTGAGAC ATCTGCTGCA TACCGCTACT ATATTGATGA TGTTTTCCAT 
GCCCTTGTAC GGGAGATACG TAGGAAAGAA AAGGAGGCAG TACTGGCCAT GGAGAAAAAA 
TCTAAGCCCA AAAACAGTGT ATGGAAGAGG CTAAAATCAC CATTCCGGAA GAAGAAAGAT 
TCAGTAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243749.1
CDS	110..661
Translation	MTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

Target ORF information:

RefSeq Version	NM_001256820.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256820.2

ORF Insert Sequence:

ATGACCATGC AGTTCATCAG CCACCGATTC CCAGAAGATC ATGATCCCAC CATTGAAGAT 
GCTTATAAGA TCAGGATCCG TATTGATGAT GAGCCTGCCA ATCTGGACAT TTTGGATACA 
GCTGGACAGG CAGAGTTTAC AGCCATGCGG GACCAGTATA TGAGGGCAGG AGAAGGGTTT 
ATCATCTGTT ACTCTATCAC GGATCGTCGA AGTTTCCATG AAGTTCGTGA GTTTAAACAG 
CTTATTTATC GAGTCCGACG TACTGACGAT ACACCTGTGG TTCTTGTGGG AAACAAGTCA 
GACCTCAAAC AGCTAAGACA GGTCACCAAG GAAGAAGGAT TGGCCTTGGC CCGAGAATTC 
AGCTGTCCCT TTTTTGAGAC ATCTGCTGCA TACCGCTACT ATATTGATGA TGTTTTCCAT 
GCCCTTGTAC GGGAGATACG TAGGAAAGAA AAGGAGGCAG TACTGGCCAT GGAGAAAAAA 
TCTAAGCCCA AAAACAGTGT ATGGAAGAGG CTAAAATCAC CATTCCGGAA GAAGAAAGAT 
TCAGTAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIT1 ( NM_006912.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_008843.1 Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22544
Clone ID Related Accession (Same CDS sequence)	NM_006912.5 , NM_006912.6
Accession Version	NM_006912.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	GTP-binding protein Rit1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298768.1, BC104186.2, AL355388.30, AL139128.24 and BU674989.1. This sequence is a reference standard in the RefSeqGene project. On Feb 28, 2012 this sequence version replaced NM_006912.4. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences. These difference cause translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.246186.1, SRR1803612.72073.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCTG GAACTCGCCC AGTTGGTAGC TGCTGTAGCA GCCCCGCTGG GCTCTCACGG 
GAGTACAAAC TAGTGATGCT GGGTGCTGGT GGTGTAGGGA AGAGTGCCAT GACCATGCAG 
TTCATCAGCC ACCGATTCCC AGAAGATCAT GATCCCACCA TTGAAGATGC TTATAAGATC 
AGGATCCGTA TTGATGATGA GCCTGCCAAT CTGGACATTT TGGATACAGC TGGACAGGCA 
GAGTTTACAG CCATGCGGGA CCAGTATATG AGGGCAGGAG AAGGGTTTAT CATCTGTTAC 
TCTATCACGG ATCGTCGAAG TTTCCATGAA GTTCGTGAGT TTAAACAGCT TATTTATCGA 
GTCCGACGTA CTGACGATAC ACCTGTGGTT CTTGTGGGAA ACAAGTCAGA CCTCAAACAG 
CTAAGACAGG TCACCAAGGA AGAAGGATTG GCCTTGGCCC GAGAATTCAG CTGTCCCTTT 
TTTGAGACAT CTGCTGCATA CCGCTACTAT ATTGATGATG TTTTCCATGC CCTTGTACGG 
GAGATACGTA GGAAAGAAAA GGAGGCAGTA CTGGCCATGG AGAAAAAATC TAAGCCCAAA 
AACAGTGTAT GGAAGAGGCT AAAATCACCA TTCCGGAAGA AGAAAGATTC AGTAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008843.1
CDS	204..863
Translation	MDSGTRPVGSCCSSPAGLSREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

Target ORF information:

RefSeq Version	NM_006912.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006912.5

ORF Insert Sequence:

ATGGATTCTG GAACTCGCCC AGTTGGTAGC TGCTGTAGCA GCCCCGCTGG GCTCTCACGG 
GAGTACAAAC TAGTGATGCT GGGTGCTGGT GGTGTAGGGA AGAGTGCCAT GACCATGCAG 
TTCATCAGCC ACCGATTCCC AGAAGATCAT GATCCCACCA TTGAAGATGC TTATAAGATC 
AGGATCCGTA TTGATGATGA GCCTGCCAAT CTGGACATTT TGGATACAGC TGGACAGGCA 
GAGTTTACAG CCATGCGGGA CCAGTATATG AGGGCAGGAG AAGGGTTTAT CATCTGTTAC 
TCTATCACGG ATCGTCGAAG TTTCCATGAA GTTCGTGAGT TTAAACAGCT TATTTATCGA 
GTCCGACGTA CTGACGATAC ACCTGTGGTT CTTGTGGGAA ACAAGTCAGA CCTCAAACAG 
CTAAGACAGG TCACCAAGGA AGAAGGATTG GCCTTGGCCC GAGAATTCAG CTGTCCCTTT 
TTTGAGACAT CTGCTGCATA CCGCTACTAT ATTGATGATG TTTTCCATGC CCTTGTACGG 
GAGATACGTA GGAAAGAAAA GGAGGCAGTA CTGGCCATGG AGAAAAAATC TAAGCCCAAA 
AACAGTGTAT GGAAGAGGCT AAAATCACCA TTCCGGAAGA AGAAAGATTC AGTAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIT1 ( NM_006912.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_008843.1 Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22544
Clone ID Related Accession (Same CDS sequence)	NM_006912.5 , NM_006912.6
Accession Version	NM_006912.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	GTP-binding protein Rit1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298768.1, BC104186.2, AL355388.30, AL139128.24 and BU674989.1. This sequence is a reference standard in the RefSeqGene project. On Feb 27, 2019 this sequence version replaced NM_006912.5. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences. These difference cause translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.276594.1, SRR1803612.3340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000368323.8/ ENSP00000357306.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATTCTG GAACTCGCCC AGTTGGTAGC TGCTGTAGCA GCCCCGCTGG GCTCTCACGG 
GAGTACAAAC TAGTGATGCT GGGTGCTGGT GGTGTAGGGA AGAGTGCCAT GACCATGCAG 
TTCATCAGCC ACCGATTCCC AGAAGATCAT GATCCCACCA TTGAAGATGC TTATAAGATC 
AGGATCCGTA TTGATGATGA GCCTGCCAAT CTGGACATTT TGGATACAGC TGGACAGGCA 
GAGTTTACAG CCATGCGGGA CCAGTATATG AGGGCAGGAG AAGGGTTTAT CATCTGTTAC 
TCTATCACGG ATCGTCGAAG TTTCCATGAA GTTCGTGAGT TTAAACAGCT TATTTATCGA 
GTCCGACGTA CTGACGATAC ACCTGTGGTT CTTGTGGGAA ACAAGTCAGA CCTCAAACAG 
CTAAGACAGG TCACCAAGGA AGAAGGATTG GCCTTGGCCC GAGAATTCAG CTGTCCCTTT 
TTTGAGACAT CTGCTGCATA CCGCTACTAT ATTGATGATG TTTTCCATGC CCTTGTACGG 
GAGATACGTA GGAAAGAAAA GGAGGCAGTA CTGGCCATGG AGAAAAAATC TAAGCCCAAA 
AACAGTGTAT GGAAGAGGCT AAAATCACCA TTCCGGAAGA AGAAAGATTC AGTAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008843.1
CDS	151..810
Translation	MDSGTRPVGSCCSSPAGLSREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

Target ORF information:

RefSeq Version	NM_006912.6
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006912.6

ORF Insert Sequence:

ATGGATTCTG GAACTCGCCC AGTTGGTAGC TGCTGTAGCA GCCCCGCTGG GCTCTCACGG 
GAGTACAAAC TAGTGATGCT GGGTGCTGGT GGTGTAGGGA AGAGTGCCAT GACCATGCAG 
TTCATCAGCC ACCGATTCCC AGAAGATCAT GATCCCACCA TTGAAGATGC TTATAAGATC 
AGGATCCGTA TTGATGATGA GCCTGCCAAT CTGGACATTT TGGATACAGC TGGACAGGCA 
GAGTTTACAG CCATGCGGGA CCAGTATATG AGGGCAGGAG AAGGGTTTAT CATCTGTTAC 
TCTATCACGG ATCGTCGAAG TTTCCATGAA GTTCGTGAGT TTAAACAGCT TATTTATCGA 
GTCCGACGTA CTGACGATAC ACCTGTGGTT CTTGTGGGAA ACAAGTCAGA CCTCAAACAG 
CTAAGACAGG TCACCAAGGA AGAAGGATTG GCCTTGGCCC GAGAATTCAG CTGTCCCTTT 
TTTGAGACAT CTGCTGCATA CCGCTACTAT ATTGATGATG TTTTCCATGC CCTTGTACGG 
GAGATACGTA GGAAAGAAAA GGAGGCAGTA CTGGCCATGG AGAAAAAATC TAAGCCCAAA 
AACAGTGTAT GGAAGAGGCT AAAATCACCA TTCCGGAAGA AGAAAGATTC AGTAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIT1 ( NM_001256821.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243750.1 Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12558
Clone ID Related Accession (Same CDS sequence)	NM_001256821.1 , NM_001256821.2
Accession Version	NM_001256821.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	GTP-binding protein Rit1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ918446.1, BI916790.1, AL355388.30, AL139128.24 and BU674989.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ918446.1, BI916790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGGT GGCTTTTCCT CGGGGCAACC GAGGAAGGCC CCAAGAGGAC AATGGATTCT 
GGAACTCGCC CAGTTGGTAG CTGCTGTAGC AGCCCCGCTG GGCTCTCACG GGAGTACAAA 
CTAGTGATGC TGGGTGCTGG TGGTGTAGGG AAGAGTGCCA TGACCATGCA GTTCATCAGC 
CACCGATTCC CAGAAGATCA TGATCCCACC ATTGAAGATG CTTATAAGAT CAGGATCCGT 
ATTGATGATG AGCCTGCCAA TCTGGACATT TTGGATACAG CTGGACAGGC AGAGTTTACA 
GCCATGCGGG ACCAGTATAT GAGGGCAGGA GAAGGGTTTA TCATCTGTTA CTCTATCACG 
GATCGTCGAA GTTTCCATGA AGTTCGTGAG TTTAAACAGC TTATTTATCG AGTCCGACGT 
ACTGACGATA CACCTGTGGT TCTTGTGGGA AACAAGTCAG ACCTCAAACA GCTAAGACAG 
GTCACCAAGG AAGAAGGATT GGCCTTGGCC CGAGAATTCA GCTGTCCCTT TTTTGAGACA 
TCTGCTGCAT ACCGCTACTA TATTGATGAT GTTTTCCATG CCCTTGTACG GGAGATACGT 
AGGAAAGAAA AGGAGGCAGT ACTGGCCATG GAGAAAAAAT CTAAGCCCAA AAACAGTGTA 
TGGAAGAGGC TAAAATCACC ATTCCGGAAG AAGAAAGATT CAGTAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243750.1
CDS	31..741
Translation	MERWLFLGATEEGPKRTMDSGTRPVGSCCSSPAGLSREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

Target ORF information:

RefSeq Version	NM_001256821.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256821.1

ORF Insert Sequence:

ATGGAAAGGT GGCTTTTCCT CGGGGCAACC GAGGAAGGCC CCAAGAGGAC AATGGATTCT 
GGAACTCGCC CAGTTGGTAG CTGCTGTAGC AGCCCCGCTG GGCTCTCACG GGAGTACAAA 
CTAGTGATGC TGGGTGCTGG TGGTGTAGGG AAGAGTGCCA TGACCATGCA GTTCATCAGC 
CACCGATTCC CAGAAGATCA TGATCCCACC ATTGAAGATG CTTATAAGAT CAGGATCCGT 
ATTGATGATG AGCCTGCCAA TCTGGACATT TTGGATACAG CTGGACAGGC AGAGTTTACA 
GCCATGCGGG ACCAGTATAT GAGGGCAGGA GAAGGGTTTA TCATCTGTTA CTCTATCACG 
GATCGTCGAA GTTTCCATGA AGTTCGTGAG TTTAAACAGC TTATTTATCG AGTCCGACGT 
ACTGACGATA CACCTGTGGT TCTTGTGGGA AACAAGTCAG ACCTCAAACA GCTAAGACAG 
GTCACCAAGG AAGAAGGATT GGCCTTGGCC CGAGAATTCA GCTGTCCCTT TTTTGAGACA 
TCTGCTGCAT ACCGCTACTA TATTGATGAT GTTTTCCATG CCCTTGTACG GGAGATACGT 
AGGAAAGAAA AGGAGGCAGT ACTGGCCATG GAGAAAAAAT CTAAGCCCAA AAACAGTGTA 
TGGAAGAGGC TAAAATCACC ATTCCGGAAG AAGAAAGATT CAGTAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIT1 ( NM_001256821.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243750.1 Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 1, mRNA.

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CloneID	OHu12558
Clone ID Related Accession (Same CDS sequence)	NM_001256821.1 , NM_001256821.2
Accession Version	NM_001256821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	GTP-binding protein Rit1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355388.30, BQ918446.1, BI916790.1, AL139128.24 and BU674989.1. On May 31, 2019 this sequence version replaced NM_001256821.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.667491.1, BI916790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGGT GGCTTTTCCT CGGGGCAACC GAGGAAGGCC CCAAGAGGAC AATGGATTCT 
GGAACTCGCC CAGTTGGTAG CTGCTGTAGC AGCCCCGCTG GGCTCTCACG GGAGTACAAA 
CTAGTGATGC TGGGTGCTGG TGGTGTAGGG AAGAGTGCCA TGACCATGCA GTTCATCAGC 
CACCGATTCC CAGAAGATCA TGATCCCACC ATTGAAGATG CTTATAAGAT CAGGATCCGT 
ATTGATGATG AGCCTGCCAA TCTGGACATT TTGGATACAG CTGGACAGGC AGAGTTTACA 
GCCATGCGGG ACCAGTATAT GAGGGCAGGA GAAGGGTTTA TCATCTGTTA CTCTATCACG 
GATCGTCGAA GTTTCCATGA AGTTCGTGAG TTTAAACAGC TTATTTATCG AGTCCGACGT 
ACTGACGATA CACCTGTGGT TCTTGTGGGA AACAAGTCAG ACCTCAAACA GCTAAGACAG 
GTCACCAAGG AAGAAGGATT GGCCTTGGCC CGAGAATTCA GCTGTCCCTT TTTTGAGACA 
TCTGCTGCAT ACCGCTACTA TATTGATGAT GTTTTCCATG CCCTTGTACG GGAGATACGT 
AGGAAAGAAA AGGAGGCAGT ACTGGCCATG GAGAAAAAAT CTAAGCCCAA AAACAGTGTA 
TGGAAGAGGC TAAAATCACC ATTCCGGAAG AAGAAAGATT CAGTAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243750.1
CDS	34..744
Translation	MERWLFLGATEEGPKRTMDSGTRPVGSCCSSPAGLSREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPANLDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

Target ORF information:

RefSeq Version	NM_001256821.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras like without CAAX 1 (RIT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256821.2

ORF Insert Sequence:

ATGGAAAGGT GGCTTTTCCT CGGGGCAACC GAGGAAGGCC CCAAGAGGAC AATGGATTCT 
GGAACTCGCC CAGTTGGTAG CTGCTGTAGC AGCCCCGCTG GGCTCTCACG GGAGTACAAA 
CTAGTGATGC TGGGTGCTGG TGGTGTAGGG AAGAGTGCCA TGACCATGCA GTTCATCAGC 
CACCGATTCC CAGAAGATCA TGATCCCACC ATTGAAGATG CTTATAAGAT CAGGATCCGT 
ATTGATGATG AGCCTGCCAA TCTGGACATT TTGGATACAG CTGGACAGGC AGAGTTTACA 
GCCATGCGGG ACCAGTATAT GAGGGCAGGA GAAGGGTTTA TCATCTGTTA CTCTATCACG 
GATCGTCGAA GTTTCCATGA AGTTCGTGAG TTTAAACAGC TTATTTATCG AGTCCGACGT 
ACTGACGATA CACCTGTGGT TCTTGTGGGA AACAAGTCAG ACCTCAAACA GCTAAGACAG 
GTCACCAAGG AAGAAGGATT GGCCTTGGCC CGAGAATTCA GCTGTCCCTT TTTTGAGACA 
TCTGCTGCAT ACCGCTACTA TATTGATGAT GTTTTCCATG CCCTTGTACG GGAGATACGT 
AGGAAAGAAA AGGAGGCAGT ACTGGCCATG GAGAAAAAAT CTAAGCCCAA AAACAGTGTA 
TGGAAGAGGC TAAAATCACC ATTCCGGAAG AAGAAAGATT CAGTAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expanding the cardiac spectrum of Noonan syndrome with RIT1 variant: Left main coronary artery atresia causing sudden death.
European journal of medical genetics60(6)299-302(2017 Jun)
Ramond F,Duband S,Croisille P,Cav? H,Teyssier G,Adouard V,Touraine R

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RIT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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