GRK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK1
Entrez Gene ID	6011
Full Name	G protein-coupled receptor kinase 1
Synonyms	GPRK1,RHOK,RK
General protein information	Preferred Names G protein-coupled receptor kinase 1 Names rhodopsin kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q34
Summary	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008].
Disorder MIM:	180381
Disorder Html:	Oguchi disease-2, 613411 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002929.3	NP_002920.1	rhodopsin kinase GRK1
XM_017020684.1	XP_016876173.1	rhodopsin kinase GRK1 isoform X1
NM_002929.2	NP_002920.1	rhodopsin kinase

Pathways from BioSystems

Homologies

	GRK1	XP_001105738.1
Rattus norvegicus (Norway rat)	Grk1	NP_112358.1
Danio rerio (zebrafish)	grk1a	NP_001029353.2
Pan troglodytes (chimpanzee)	GRK1	XP_003314275.1
Mus musculus (house mouse)	Grk1	NP_036011.3
Homo sapiens (human)	GRK1	NP_002920.1
Canis lupus familiaris (dog)	GRK1	XP_003639862.1
Xenopus tropicalis (tropical clawed frog)	grk1	XP_002937018.2
Bos taurus (cattle)	GRK1	NP_776598.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel GRK1 mutation in an Italian patient with Oguchi disease.
Mucciolo DP, Sodi A, Murro V, Passerini I, Palchetti S, Pelo E, Virgili G, Rizzo S
Ophthalmic genetics39(1)137-138(2018 Jan-Feb)

A novel missense mutation of the GRK1 gene in Oguchi disease.
Teke MY, Citirik M, Kabacam S, Demircan S, Alikasifoglu M
Molecular medicine reports14(4)3129-33(2016 Oct)

AAMP Regulates Endothelial Cell Migration and Angiogenesis Through RhoA/Rho Kinase Signaling.
Hu J, Qiu J, Zheng Y, Zhang T, Yin T, Xie X, Wang G
Annals of biomedical engineering44(5)1462-74(2016 May)

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
Pandalaneni S, Karuppiah V, Saleem M, Haynes LP, Burgoyne RD, Mayans O, Derrick JP, Lian LY
The Journal of biological chemistry290(30)18744-56(2015 Jul)

The first case of Oguchi disease, type 2 in a Polish patient with confirmed GRK1 gene mutation.
Skorczyk-Werner A, Koci?cki J, Wawrocka A, Wicher K, Krawczy?iski MR
Klinika oczna117(1)27-30(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gene analysis determined a novel GRK1 mutation c.923T>C, which caused Oguchi disease in all siblings. This mutation, was demonstrated by amino acid alignment analysis to be in a phylogenetically conserved region and resulted in an amino acid change from leucine to proline at position 308. Thus, the present study reports a novel missense mutation of GRK1 in the affected members of a consanguineous Turkish family.
Title: A novel missense mutation of the GRK1 gene in Oguchi disease.

AAMP Regulates Endothelial Cell Migration and Angiogenesis Through RhoA/Rho Kinase Signaling.
Title: AAMP Regulates Endothelial Cell Migration and Angiogenesis Through RhoA/Rho Kinase Signaling.

In the Ca(2+)/NCS-1.D2R peptide complex, the C-terminal region adopts a 310 helix-turn-310 helix, whereas in the GRK1 peptide complex it forms an a-helix
Title: Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.

The identification of the c.1607_1610delCGGA mutation in a patient with Oguchi disease confirms the pathogenicity of this variant.
Title: The first case of Oguchi disease, type 2 in a Polish patient with confirmed GRK1 gene mutation.

Rho-kinase activity exhibits distinct circadian variation associated with alterations in coronary vasomotor responses and autonomic activity in VSA patients.
Title: Circadian variation of Rho-kinase activity in circulating leukocytes of patients with vasospastic angina.

The following GRK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17143	NM_002929.2 Latest version!	Homo sapiens G protein-coupled receptor kinase 1 (GRK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu84601	XM_017020684.1 Latest version!	Homo sapiens G protein-coupled receptor kinase 1 (GRK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu107525	NM_002929.3 Latest version!	Homo sapiens G protein-coupled receptor kinase 1 (GRK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK1 ( NM_002929.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002920.1 Homo sapiens G protein-coupled receptor kinase 1 (GRK1), mRNA.

Price & Availability↑

CloneID	OHu17143
Clone ID Related Accession (Same CDS sequence)	NM_002929.2
Accession Version	NM_002929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	rhodopsin kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U63973.1, BM702747.1 and BX647503.1. This sequence is a reference standard in the RefSeqGene project. On Sep 3, 2004 this sequence version replaced NM_002929.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U63973.1, AK289912.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTCG GGTCTTTGGA GACCGTGGTG GCCAACTCTG CCTTCATCGC CGCCCGAGGC 
AGCTTTGACG GCAGCAGCTC CCAACCCTCC CGGGACAAGA AGTACCTGGC CAAGCTCAAG 
CTGCCCCCGC TGTCCAAGTG TGAGTCCCTC CGCGACAGCC TCAGCCTGGA GTTTGAGAGT 
GTGTGCTTGG AGCAGCCCAT CGGCAAGAAG CTCTTTCAGC AGTTCCTACA ATCGGCAGAG 
AAGCACCTGC CGGCCCTGGA GCTCTGGAAA GACATCGAGG ACTATGACAC GGCAGACAAT 
GACCTCCAGC CACAGAAGGC CCAGACCATC CTGGCCCAGT ACCTGGACCC CCAGGCCAAA 
CTCTTCTGCA GCTTCCTGGA TGAGGGGATA GTGGCGAAGT TTAAGGAGGG GCCTGTGGAG 
ATCCAGGACG GGCTCTTCCA GCCCCTGCTG CAGGCCACCC TGGCACACCT GGGCCAAGCC 
CCCTTCCAGG AGTACCTGGG CAGCCTGTAC TTCCTGAGGT TCCTGCAGTG GAAGTGGCTG 
GAAGCCCAGC CCATGGGGGA GGACTGGTTC CTGGACTTCA GGGTCCTGGG GAAAGGGGGC 
TTCGGGGAGG TGTCGGCCTG CCAGATGAAG GCGACCGGCA AGCTGTATGC CTGCAAGAAG 
CTGAACAAGA AGCGGCTGAA GAAGAGGAAG GGCTACCAGG GTGCTATGGT GGAGAAGAAG 
ATTCTGATGA AAGTACACAG CAGGTTCATC GTGTCTCTGG CCTATGCGTT TGAAACCAAA 
GCCGACCTCT GTCTGGTGAT GACCATCATG AACGGAGGTG ACATCAGGTA CCACATCTAC 
AACGTGAATG AGGAGAACCC TGGCTTCCCG GAGCCGCGCG CCCTCTTCTA CACGGCGCAG 
ATCATCTGCG GCCTGGAGCA CCTGCACCAG AGGCGGATCG TCTACCGCGA CCTCAAGCCC 
GAGAACGTGC TGCTGGACAA TGACGGCAAT GTCCGGATCT CTGACCTTGG GCTGGCCGTG 
GAGCTGCTGG ACGGACAGAG CAAGACCAAG GGCTACGCAG GGACCCCAGG TTTCATGGCC 
CCCGAGCTCC TGCAGGGCGA GGAGTACGAC TTCTCCGTGG ACTACTTTGC CCTGGGGGTC 
ACCCTGTATG AGATGATTGC GGCCAGAGGA CCCTTCCGAG CCCGTGGAGA GAAGGTGGAG 
AACAAGGAGC TGAAGCACCG GATCATCTCA GAGCCCGTGA AGTACCCTGA TAAGTTCAGC 
CAGGCCAGCA AGGACTTCTG CGAGGCGCTG CTGGAGAAGG ACCCGGAGAA GCGCCTGGGG 
TTCAGAGATG AGACCTGCGA CAAGCTCCGT GCCCACCCCC TCTTCAAGGA CCTTAACTGG 
AGGCAGCTGG AGGCTGGGAT GCTGATGCCC CCTTTCATCC CAGACTCCAA AACTGTCTAC 
GCAAAGGATA TTCAGGACGT GGGTGCCTTT TCCACCGTCA AAGGTGTGGC CTTTGACAAA 
ACAGACACAG AATTCTTTCA GGAATTTGCC ACTGGCAACT GCCCCATCCC CTGGCAGGAG 
GAGATGATCG AGACGGGCAT CTTTGGCGAG CTGAACGTGT GGCGCTCGGA CGGTCAGATG 
CCGGACGACA TGAAGGGCAT CTCCGGGGGC TCCAGCTCCT CGTCCAAGTC AGGGATGTGT 
CTGGTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002920.1
CDS	109..1800
Translation	MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKKLFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVEIQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIYNVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

Target ORF information:

RefSeq Version	NM_002929.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 1 (GRK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002929.2

ORF Insert Sequence:

ATGGATTTCG GGTCTTTGGA GACCGTGGTG GCCAACTCTG CCTTCATCGC CGCCCGAGGC 
AGCTTTGACG GCAGCAGCTC CCAACCCTCC CGGGACAAGA AGTACCTGGC CAAGCTCAAG 
CTGCCCCCGC TGTCCAAGTG TGAGTCCCTC CGCGACAGCC TCAGCCTGGA GTTTGAGAGT 
GTGTGCTTGG AGCAGCCCAT CGGCAAGAAG CTCTTTCAGC AGTTCCTACA ATCGGCAGAG 
AAGCACCTGC CGGCCCTGGA GCTCTGGAAA GACATCGAGG ACTATGACAC GGCAGACAAT 
GACCTCCAGC CACAGAAGGC CCAGACCATC CTGGCCCAGT ACCTGGACCC CCAGGCCAAA 
CTCTTCTGCA GCTTCCTGGA TGAGGGGATA GTGGCGAAGT TTAAGGAGGG GCCTGTGGAG 
ATCCAGGACG GGCTCTTCCA GCCCCTGCTG CAGGCCACCC TGGCACACCT GGGCCAAGCC 
CCCTTCCAGG AGTACCTGGG CAGCCTGTAC TTCCTGAGGT TCCTGCAGTG GAAGTGGCTG 
GAAGCCCAGC CCATGGGGGA GGACTGGTTC CTGGACTTCA GGGTCCTGGG GAAAGGGGGC 
TTCGGGGAGG TGTCGGCCTG CCAGATGAAG GCGACCGGCA AGCTGTATGC CTGCAAGAAG 
CTGAACAAGA AGCGGCTGAA GAAGAGGAAG GGCTACCAGG GTGCTATGGT GGAGAAGAAG 
ATTCTGATGA AAGTACACAG CAGGTTCATC GTGTCTCTGG CCTATGCGTT TGAAACCAAA 
GCCGACCTCT GTCTGGTGAT GACCATCATG AACGGAGGTG ACATCAGGTA CCACATCTAC 
AACGTGAATG AGGAGAACCC TGGCTTCCCG GAGCCGCGCG CCCTCTTCTA CACGGCGCAG 
ATCATCTGCG GCCTGGAGCA CCTGCACCAG AGGCGGATCG TCTACCGCGA CCTCAAGCCC 
GAGAACGTGC TGCTGGACAA TGACGGCAAT GTCCGGATCT CTGACCTTGG GCTGGCCGTG 
GAGCTGCTGG ACGGACAGAG CAAGACCAAG GGCTACGCAG GGACCCCAGG TTTCATGGCC 
CCCGAGCTCC TGCAGGGCGA GGAGTACGAC TTCTCCGTGG ACTACTTTGC CCTGGGGGTC 
ACCCTGTATG AGATGATTGC GGCCAGAGGA CCCTTCCGAG CCCGTGGAGA GAAGGTGGAG 
AACAAGGAGC TGAAGCACCG GATCATCTCA GAGCCCGTGA AGTACCCTGA TAAGTTCAGC 
CAGGCCAGCA AGGACTTCTG CGAGGCGCTG CTGGAGAAGG ACCCGGAGAA GCGCCTGGGG 
TTCAGAGATG AGACCTGCGA CAAGCTCCGT GCCCACCCCC TCTTCAAGGA CCTTAACTGG 
AGGCAGCTGG AGGCTGGGAT GCTGATGCCC CCTTTCATCC CAGACTCCAA AACTGTCTAC 
GCAAAGGATA TTCAGGACGT GGGTGCCTTT TCCACCGTCA AAGGTGTGGC CTTTGACAAA 
ACAGACACAG AATTCTTTCA GGAATTTGCC ACTGGCAACT GCCCCATCCC CTGGCAGGAG 
GAGATGATCG AGACGGGCAT CTTTGGCGAG CTGAACGTGT GGCGCTCGGA CGGTCAGATG 
CCGGACGACA TGAAGGGCAT CTCCGGGGGC TCCAGCTCCT CGTCCAAGTC AGGGATGTGT 
CTGGTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK1 ( XM_017020684.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876173.1 Homo sapiens G protein-coupled receptor kinase 1 (GRK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu84601
Clone ID Related Accession (Same CDS sequence)	XM_017020684.1
Accession Version	XM_017020684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rhodopsin kinase GRK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGAAGATTCT GATGAAAGTA CACAGCAGGT TCATCGTGTC TCTGGCCTAT 
GCGTTTGAAA CCAAAGCCGA CCTCTGTCTG GTGATGACCA TCATGAACGG AGGTGACATC 
AGGTACCACA TCTACAACGT GAATGAGGAG AACCCTGGCT TCCCGGAGCC GCGCGCCCTC 
TTCTACACGG CGCAGATCAT CTGCGGCCTG GAGCACCTGC ACCAGAGGCG GATCGTCTAC 
CGCGACCTCA AGCCCGAGAA CGTGCTGCTG GACAATGACG GCAATGTCCG GATCTCTGAC 
CTTGGGCTGG CCGTGGAGCT GCTGGACGGA CAGAGCAAGA CCAAGGGCTA CGCAGGGACC 
CCAGGTTTCA TGGCCCCCGA GCTCCTGCAG GGCGAGGAGT ACGACTTCTC CGTGGACTAC 
TTTGCCCTGG GGGTCACCCT GTATGAGATG ATTGCGGCCA GAGGACCCTT CCGAGCCCGT 
GGAGAGAAGG TGGAGAACAA GGAGCTGAAG CACCGGATCA TCTCAGAGCC CGTGAAGTAC 
CCTGATAAGT TCAGCCAGGC CAGCAAGGAC TTCTGCGAGG CGCTGCTGGA GAAGGACCCG 
GAGAAGCGCC TGGGGTTCAG AGATGAGACC TGCGACAAGC TCCGTGCCCA CCCCCTCTTC 
AAGGACCTTA ACTGGAGGCA GCTGGAGGCT GGGATGCTGA TGCCCCCTTT CATCCCAGAC 
TCCAAAACTG TCTACGCAAA GGATATTCAG GACGTGGGTG CCTTTTCCAC CGTCAAAGGT 
GTGGCCTTTG ACAAAACAGA CACAGAATTC TTTCAGGAAT TTGCCACTGG CAACTGCCCC 
ATCCCCTGGC AGGAGGAGAT GATCGAGACG GGCATCTTTG GCGAGCTGAA CGTGTGGCGC 
TCGGACGGTC AGATGCCGGA CGACATGAAG GGCATCTCCG GGGGCTCCAG CTCCTCGTCC 
AAGTCAGGGA TGTGTCTGGT TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876173.1
CDS	2481..3467
Translation	MVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIYNVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

Target ORF information:

RefSeq Version	XM_017020684.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 1 (GRK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020684.1

ORF Insert Sequence:

ATGGTGGAGA AGAAGATTCT GATGAAAGTA CACAGCAGGT TCATCGTGTC TCTGGCCTAT 
GCGTTTGAAA CCAAAGCCGA CCTCTGTCTG GTGATGACCA TCATGAACGG AGGTGACATC 
AGGTACCACA TCTACAACGT GAATGAGGAG AACCCTGGCT TCCCGGAGCC GCGCGCCCTC 
TTCTACACGG CGCAGATCAT CTGCGGCCTG GAGCACCTGC ACCAGAGGCG GATCGTCTAC 
CGCGACCTCA AGCCCGAGAA CGTGCTGCTG GACAATGACG GCAATGTCCG GATCTCTGAC 
CTTGGGCTGG CCGTGGAGCT GCTGGACGGA CAGAGCAAGA CCAAGGGCTA CGCAGGGACC 
CCAGGTTTCA TGGCCCCCGA GCTCCTGCAG GGCGAGGAGT ACGACTTCTC CGTGGACTAC 
TTTGCCCTGG GGGTCACCCT GTATGAGATG ATTGCGGCCA GAGGACCCTT CCGAGCCCGT 
GGAGAGAAGG TGGAGAACAA GGAGCTGAAG CACCGGATCA TCTCAGAGCC CGTGAAGTAC 
CCTGATAAGT TCAGCCAGGC CAGCAAGGAC TTCTGCGAGG CGCTGCTGGA GAAGGACCCG 
GAGAAGCGCC TGGGGTTCAG AGATGAGACC TGCGACAAGC TCCGTGCCCA CCCCCTCTTC 
AAGGACCTTA ACTGGAGGCA GCTGGAGGCT GGGATGCTGA TGCCCCCTTT CATCCCAGAC 
TCCAAAACTG TCTACGCAAA GGATATTCAG GACGTGGGTG CCTTTTCCAC CGTCAAAGGT 
GTGGCCTTTG ACAAAACAGA CACAGAATTC TTTCAGGAAT TTGCCACTGG CAACTGCCCC 
ATCCCCTGGC AGGAGGAGAT GATCGAGACG GGCATCTTTG GCGAGCTGAA CGTGTGGCGC 
TCGGACGGTC AGATGCCGGA CGACATGAAG GGCATCTCCG GGGGCTCCAG CTCCTCGTCC 
AAGTCAGGGA TGTGTCTGGT TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK1 ( NM_002929.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002920.1 Homo sapiens G protein-coupled receptor kinase 1 (GRK1), mRNA.

Price & Availability↑

CloneID	OHu107525
Clone ID Related Accession (Same CDS sequence)	NM_002929.3
Accession Version	NM_002929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	rhodopsin kinase GRK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX537316.2 and AC187648.1. On Nov 23, 2018 this sequence version replaced NM_002929.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289912.1, U63973.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000335678.7/ ENSP00000334876.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTTCG GGTCTTTGGA GACCGTGGTG GCCAACTCTG CCTTCATCGC CGCCCGAGGC 
AGCTTTGACG GCAGCAGCTC CCAACCCTCC CGGGACAAGA AGTACCTGGC CAAGCTCAAG 
CTGCCCCCGC TGTCCAAGTG TGAGTCCCTC CGCGACAGCC TCAGCCTGGA GTTTGAGAGT 
GTGTGCTTGG AGCAGCCCAT CGGCAAGAAG CTCTTTCAGC AGTTCCTACA ATCGGCAGAG 
AAGCACCTGC CGGCCCTGGA GCTCTGGAAA GACATCGAGG ACTATGACAC GGCAGACAAT 
GACCTCCAGC CACAGAAGGC CCAGACCATC CTGGCCCAGT ACCTGGACCC CCAGGCCAAA 
CTCTTCTGCA GCTTCCTGGA TGAGGGGATA GTGGCGAAGT TTAAGGAGGG GCCTGTGGAG 
ATCCAGGACG GGCTCTTCCA GCCCCTGCTG CAGGCCACCC TGGCACACCT GGGCCAAGCC 
CCCTTCCAGG AGTACCTGGG CAGCCTGTAC TTCCTGAGGT TCCTGCAGTG GAAGTGGCTG 
GAAGCCCAGC CCATGGGGGA GGACTGGTTC CTGGACTTCA GGGTCCTAGG GAAAGGGGGC 
TTCGGGGAGG TGTCGGCCTG CCAGATGAAG GCGACCGGCA AGCTGTATGC CTGCAAGAAG 
CTGAACAAGA AGCGGCTGAA GAAGAGGAAG GGCTACCAGG GTGCTATGGT GGAGAAGAAG 
ATTCTGATGA AAGTACACAG CAGGTTCATC GTGTCTCTGG CCTATGCGTT TGAAACCAAA 
GCCGACCTCT GTCTGGTGAT GACCATCATG AACGGAGGTG ACATCAGGTA CCACATCTAC 
AACGTGAATG AGGAGAACCC TGGCTTCCCG GAGCCGCGCG CCCTCTTCTA CACGGCGCAG 
ATCATCTGCG GCCTGGAGCA CCTGCACCAG AGGCGGATCG TCTACCGCGA CCTCAAGCCC 
GAGAACGTGC TGCTGGACAA TGACGGCAAT GTCCGGATCT CTGACCTTGG GCTGGCCGTG 
GAGCTGCTGG ACGGACAGAG CAAGACCAAG GGCTACGCAG GGACCCCAGG TTTCATGGCC 
CCCGAGCTCC TGCAGGGCGA GGAGTACGAC TTCTCCGTGG ACTACTTTGC CCTGGGGGTC 
ACCCTGTATG AGATGATTGC GGCCAGAGGA CCCTTCCGAG CCCGTGGAGA GAAGGTGGAG 
AACAAGGAGC TGAAGCACCG GATCATCTCA GAGCCCGTGA AGTACCCTGA TAAGTTCAGC 
CAGGCCAGCA AGGACTTCTG CGAGGCGCTG CTGGAGAAGG ACCCGGAGAA GCGCCTGGGG 
TTCAGAGATG AGACCTGCGA CAAGCTCCGT GCCCACCCCC TCTTCAAGGA CCTTAACTGG 
AGGCAGCTGG AGGCTGGGAT GCTGATGCCC CCTTTCATCC CAGACTCCAA AACTGTCTAC 
GCAAAGGATA TTCAGGACGT GGGTGCCTTT TCCACCGTCA AAGGTGTGGC CTTTGACAAA 
ACAGACACAG AATTCTTTCA GGAATTTGCC ACTGGCAACT GCCCCATCCC CTGGCAGGAG 
GAGATGATCG AGACGGGCAT CTTTGGCGAG CTGAACGTGT GGCGCTCGGA CGGTCAGATG 
CCGGACGACA TGAAGGGCAT CTCCGGGGGC TCCAGCTCCT CGTCCAAGTC AGGGATGTGT 
CTGGTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002920.1
CDS	169..1860
Translation	MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKKLFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVEIQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIYNVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

Target ORF information:

RefSeq Version	NM_002929.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 1 (GRK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002929.3

ORF Insert Sequence:

ATGGATTTCG GGTCTTTGGA GACCGTGGTG GCCAACTCTG CCTTCATCGC CGCCCGAGGC 
AGCTTTGACG GCAGCAGCTC CCAACCCTCC CGGGACAAGA AGTACCTGGC CAAGCTCAAG 
CTGCCCCCGC TGTCCAAGTG TGAGTCCCTC CGCGACAGCC TCAGCCTGGA GTTTGAGAGT 
GTGTGCTTGG AGCAGCCCAT CGGCAAGAAG CTCTTTCAGC AGTTCCTACA ATCGGCAGAG 
AAGCACCTGC CGGCCCTGGA GCTCTGGAAA GACATCGAGG ACTATGACAC GGCAGACAAT 
GACCTCCAGC CACAGAAGGC CCAGACCATC CTGGCCCAGT ACCTGGACCC CCAGGCCAAA 
CTCTTCTGCA GCTTCCTGGA TGAGGGGATA GTGGCGAAGT TTAAGGAGGG GCCTGTGGAG 
ATCCAGGACG GGCTCTTCCA GCCCCTGCTG CAGGCCACCC TGGCACACCT GGGCCAAGCC 
CCCTTCCAGG AGTACCTGGG CAGCCTGTAC TTCCTGAGGT TCCTGCAGTG GAAGTGGCTG 
GAAGCCCAGC CCATGGGGGA GGACTGGTTC CTGGACTTCA GGGTCCTAGG GAAAGGGGGC 
TTCGGGGAGG TGTCGGCCTG CCAGATGAAG GCGACCGGCA AGCTGTATGC CTGCAAGAAG 
CTGAACAAGA AGCGGCTGAA GAAGAGGAAG GGCTACCAGG GTGCTATGGT GGAGAAGAAG 
ATTCTGATGA AAGTACACAG CAGGTTCATC GTGTCTCTGG CCTATGCGTT TGAAACCAAA 
GCCGACCTCT GTCTGGTGAT GACCATCATG AACGGAGGTG ACATCAGGTA CCACATCTAC 
AACGTGAATG AGGAGAACCC TGGCTTCCCG GAGCCGCGCG CCCTCTTCTA CACGGCGCAG 
ATCATCTGCG GCCTGGAGCA CCTGCACCAG AGGCGGATCG TCTACCGCGA CCTCAAGCCC 
GAGAACGTGC TGCTGGACAA TGACGGCAAT GTCCGGATCT CTGACCTTGG GCTGGCCGTG 
GAGCTGCTGG ACGGACAGAG CAAGACCAAG GGCTACGCAG GGACCCCAGG TTTCATGGCC 
CCCGAGCTCC TGCAGGGCGA GGAGTACGAC TTCTCCGTGG ACTACTTTGC CCTGGGGGTC 
ACCCTGTATG AGATGATTGC GGCCAGAGGA CCCTTCCGAG CCCGTGGAGA GAAGGTGGAG 
AACAAGGAGC TGAAGCACCG GATCATCTCA GAGCCCGTGA AGTACCCTGA TAAGTTCAGC 
CAGGCCAGCA AGGACTTCTG CGAGGCGCTG CTGGAGAAGG ACCCGGAGAA GCGCCTGGGG 
TTCAGAGATG AGACCTGCGA CAAGCTCCGT GCCCACCCCC TCTTCAAGGA CCTTAACTGG 
AGGCAGCTGG AGGCTGGGAT GCTGATGCCC CCTTTCATCC CAGACTCCAA AACTGTCTAC 
GCAAAGGATA TTCAGGACGT GGGTGCCTTT TCCACCGTCA AAGGTGTGGC CTTTGACAAA 
ACAGACACAG AATTCTTTCA GGAATTTGCC ACTGGCAACT GCCCCATCCC CTGGCAGGAG 
GAGATGATCG AGACGGGCAT CTTTGGCGAG CTGAACGTGT GGCGCTCGGA CGGTCAGATG 
CCGGACGACA TGAAGGGCAT CTCCGGGGGC TCCAGCTCCT CGTCCAAGTC AGGGATGTGT 
CTGGTTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel GRK1 mutation in an Italian patient with Oguchi disease.
Ophthalmic genetics39(1)137-138(2018 Jan-Feb)
Mucciolo DP,Sodi A,Murro V,Passerini I,Palchetti S,Pelo E,Virgili G,Rizzo S

A novel missense mutation of the GRK1 gene in Oguchi disease.
Molecular medicine reports14(4)3129-33(2016 Oct)
Teke MY,Citirik M,Kabacam S,Demircan S,Alikasifoglu M

AAMP Regulates Endothelial Cell Migration and Angiogenesis Through RhoA/Rho Kinase Signaling.
Annals of biomedical engineering44(5)1462-74(2016 May)
Hu J,Qiu J,Zheng Y,Zhang T,Yin T,Xie X,Wang G

Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
The Journal of biological chemistry290(30)18744-56(2015 Jul)
Pandalaneni S,Karuppiah V,Saleem M,Haynes LP,Burgoyne RD,Mayans O,Derrick JP,Lian LY

The first case of Oguchi disease, type 2 in a Polish patient with confirmed GRK1 gene mutation.
Klinika oczna117(1)27-30(2015)
Skorczyk-Werner A,Koci?cki J,Wawrocka A,Wicher K,Krawczy?iski MR

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GRK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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