RFXAP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFXAP
Entrez Gene ID	5994
Full Name	regulatory factor X associated protein
General protein information	Preferred Names regulatory factor X associated protein Names regulatory factor X-associated protein RFX DNA-binding complex 36 kDa subunit RFX-associated protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q13.3
Summary	Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601861
Disorder Html:	Bare lymphocyte syndrome, type II, complementation group D, 209920 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000538.4	NP_000529.1	regulatory factor X-associated protein
NM_000538.3	NP_000529.1	regulatory factor X-associated protein

Pathways from BioSystems

Homologies

	RFXAP	XP_001084393.1
Pan troglodytes (chimpanzee)	RFXAP	XP_003314178.1
Mus musculus (house mouse)	Rfxap	NP_573494.1
Bos taurus (cattle)	RFXAP	XP_002691796.2
Canis lupus familiaris (dog)	RFXAP	XP_005635647.1
Rattus norvegicus (Norway rat)	Rfxap	NP_001037728.1
Homo sapiens (human)	RFXAP	NP_000529.1

Gene Ontology
Bibliography

Related articles in PubMed

Pancreatic cancer-derived exosomes transfer miRNAs to dendritic cells and inhibit RFXAP expression via miR-212-3p.
Ding G, Zhou L, Qian Y, Fu M, Chen J, Chen J, Xiang J, Wu Z, Jiang G, Cao L
Oncotarget6(30)29877-88(2015 Oct)

Solution structure of the heterotrimeric complex between the interaction domains of RFX5 and RFXAP from the RFX gene regulatory complex.
Laird KM, Briggs LL, Boss JM, Summers MF, Garvie CW
Journal of molecular biology403(1)40-51(2010 Oct)

Coordinate loss of MHC class II expression in the diffuse large B cell lymphoma cell line OCI-Ly2 is due to a novel mutation in RFX-AP.
Bushway M, Cycon KA, Mulvaney K, Murphy SP
Immunogenetics62(2)109-16(2010 Feb)

Formation of the RFX gene regulatory complex induces folding of the interaction domain of RFXAP.
Briggs L, Laird K, Boss JM, Garvie CW
Proteins76(3)655-64(2009 Aug)

Assembly of the RFX complex on the MHCII promoter: role of RFXAP and RFXB in relieving autoinhibition of RFX5.
Garvie CW, Boss JM
Biochimica et biophysica acta1779(12)797-804(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show a negative correlation between microRNA miR-212-3p and regulatory factor X-associated protein (RFXAP) in pancreatic cancer (PC) tissue.
Title: Pancreatic cancer-derived exosomes transfer miRNAs to dendritic cells and inhibit RFXAP expression via miR-212-3p.

RFXAP(C) consists of two alpha-helices that form a V-shaped structure that packs within the RFX5(N)(2) staple.
Title: Solution structure of the heterotrimeric complex between the interaction domains of RFX5 and RFXAP from the RFX gene regulatory complex.

Study demonstrates that coordinate loss of MHCII expression in OCI-Ly2 DLBCL cells is associated with an 11-base deletion in the cDNA encoding RFX-AP, that is required for activating MHCII transcription.
Title: Coordinate loss of MHC class II expression in the diffuse large B cell lymphoma cell line OCI-Ly2 is due to a novel mutation in RFX-AP.

the interaction between RFX5 and RFXAP promote folding of a poorly structured region ofRFXAP, which is required for high affinity binding of RFXB to the RFX5.RFXAP complex
Title: Formation of the RFX gene regulatory complex induces folding of the interaction domain of RFXAP.

RFXAP and RFXB have roles in relieving autoinhibition of RFX5
Title: Assembly of the RFX complex on the MHCII promoter: role of RFXAP and RFXB in relieving autoinhibition of RFX5.

The following RFXAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFXAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19548	NM_000538.4 Latest version!	Homo sapiens regulatory factor X associated protein (RFXAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu19548	NM_000538.3	Homo sapiens regulatory factor X associated protein (RFXAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFXAP ( NM_000538.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000529.1 Homo sapiens regulatory factor X associated protein (RFXAP), mRNA.

Price & Availability↑

CloneID	OHu19548
Clone ID Related Accession (Same CDS sequence)	NM_000538.4 , NM_000538.3
Accession Version	NM_000538.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	regulatory factor X-associated protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI823132.1, BC026088.1 and AL159973.16. On Dec 4, 2019 this sequence version replaced NM_000538.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026088.1, AK313912.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC AGGGTGTAGC GGAGGGCGCG GGGCCGGGCG CCGCCAGCGG CGTGCCCCAC 
CCCGCGGCCC TAGCCCCGGC TGCGGCTCCC ACCTTGGCGC CAGCCTCGGT GGCGGCCGCG 
GCCTCTCAAT TCACCCTGCT AGTGATGCAA CCCTGTGCTG GGCAGGACGA GGCTGCGGCC 
CCCGGGGGCA GCGTTGGGGC GGGCAAGCCC GTTAGGTACC TGTGCGAAGG GGCCGGGGAT 
GGCGAAGAGG AGGCTGGGGA GGACGAGGCG GACCTGTTAG ACACTTCGGA CCCTCCGGGG 
GGAGGCGAGA GCGCGGCTAG TTTGGAGGAT CTAGAGGACG AGGAGACTCA CTCGGGGGGC 
GAGGGCAGCA GCGGGGGCGC CCGGAGGCGG GGCAGCGGTG GGGGCAGCAT GAGCAAGACC 
TGCACCTACG AAGGCTGCAG CGAGACCACG AGCCAGGTGG CCAAGCAGCG CAAACCGTGG 
ATGTGCAAGA AACACCGCAA CAAGATGTAC AAGGACAAGT ATAAAAAGAA GAAGAGCGAC 
CAGGCCCTGA ACTGCGGTGG GACTGCCTCG ACTGGCAGCG CGGGAAACGT CAAACTCGAG 
GAAAGTGCAG ATAACATACT CTCCATTGTT AAACAAAGAA CAGGATCTTT TGGGGATCGT 
CCTGCAAGAC CTACTCTTTT AGAACAAGTG TTAAATCAAA AAAGACTGTC GTTACTAAGA 
AGTCCAGAAG TAGTGCAATT TTTACAGAAA CAGCAACAGC TATTAAATCA GCAAGTTTTG 
GAGCAAAGAC AACAGCAGTT TCCAGGAACA TCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000529.1
CDS	137..955
Translation	MEAQGVAEGAGPGAASGVPHPAALAPAAAPTLAPASVAAAASQFTLLVMQPCAGQDEAAAPGGSVGAGKPVRYLCEGAGDGEEEAGEDEADLLDTSDPPGGGESAASLEDLEDEETHSGGEGSSGGARRRGSGGGSMSKTCTYEGCSETTSQVAKQRKPWMCKKHRNKMYKDKYKKKKSDQALNCGGTASTGSAGNVKLEESADNILSIVKQRTGSFGDRPARPTLLEQVLNQKRLSLLRSPEVVQFLQKQQQLLNQQVLEQRQQQFPGTSM

Target ORF information:

RefSeq Version	NM_000538.4
Organism	Homo sapiens(human)
Definition	Homo sapiens regulatory factor X associated protein (RFXAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000538.4

ORF Insert Sequence:

ATGGAGGCGC AGGGTGTAGC GGAGGGCGCG GGGCCGGGCG CCGCCAGCGG CGTGCCCCAC 
CCCGCGGCCC TAGCCCCGGC TGCGGCTCCC ACCTTGGCGC CAGCCTCGGT GGCGGCCGCG 
GCCTCTCAAT TCACCCTGCT AGTGATGCAA CCCTGTGCTG GGCAGGACGA GGCTGCGGCC 
CCCGGGGGCA GCGTTGGGGC GGGCAAGCCC GTTAGGTACC TGTGCGAAGG GGCCGGGGAT 
GGCGAAGAGG AGGCTGGGGA GGACGAGGCG GACCTGTTAG ACACTTCGGA CCCTCCGGGG 
GGAGGCGAGA GCGCGGCTAG TTTGGAGGAT CTAGAGGACG AGGAGACTCA CTCGGGGGGC 
GAGGGCAGCA GCGGGGGCGC CCGGAGGCGG GGCAGCGGTG GGGGCAGCAT GAGCAAGACC 
TGCACCTACG AAGGCTGCAG CGAGACCACG AGCCAGGTGG CCAAGCAGCG CAAACCGTGG 
ATGTGCAAGA AACACCGCAA CAAGATGTAC AAGGACAAGT ATAAAAAGAA GAAGAGCGAC 
CAGGCCCTGA ACTGCGGTGG GACTGCCTCG ACTGGCAGCG CGGGAAACGT CAAACTCGAG 
GAAAGTGCAG ATAACATACT CTCCATTGTT AAACAAAGAA CAGGATCTTT TGGGGATCGT 
CCTGCAAGAC CTACTCTTTT AGAACAAGTG TTAAATCAAA AAAGACTGTC GTTACTAAGA 
AGTCCAGAAG TAGTGCAATT TTTACAGAAA CAGCAACAGC TATTAAATCA GCAAGTTTTG 
GAGCAAAGAC AACAGCAGTT TCCAGGAACA TCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFXAP ( NM_000538.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000529.1 Homo sapiens regulatory factor X associated protein (RFXAP), mRNA.

Price & Availability↑

CloneID	OHu19548
Clone ID Related Accession (Same CDS sequence)	NM_000538.4 , NM_000538.3
Accession Version	NM_000538.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	regulatory factor X-associated protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI823132.1, BC026088.1 and AL159973.16. This sequence is a reference standard in the RefSeqGene project. On May 8, 2008 this sequence version replaced NM_000538.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026088.1, SRR1163657.466531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC AGGGTGTAGC GGAGGGCGCG GGGCCGGGCG CCGCCAGCGG CGTGCCCCAC 
CCCGCGGCCC TAGCCCCGGC TGCGGCTCCC ACCTTGGCGC CAGCCTCGGT GGCGGCCGCG 
GCCTCTCAAT TCACCCTGCT AGTGATGCAA CCCTGTGCTG GGCAGGACGA GGCTGCGGCC 
CCCGGGGGCA GCGTTGGGGC GGGCAAGCCC GTTAGGTACC TGTGCGAAGG GGCCGGGGAT 
GGCGAAGAGG AGGCTGGGGA GGACGAGGCG GACCTGTTAG ACACTTCGGA CCCTCCGGGG 
GGAGGCGAGA GCGCGGCTAG TTTGGAGGAT CTAGAGGACG AGGAGACTCA CTCGGGGGGC 
GAGGGCAGCA GCGGGGGCGC CCGGAGGCGG GGCAGCGGTG GGGGCAGCAT GAGCAAGACC 
TGCACCTACG AAGGCTGCAG CGAGACCACG AGCCAGGTGG CCAAGCAGCG CAAACCGTGG 
ATGTGCAAGA AACACCGCAA CAAGATGTAC AAGGACAAGT ATAAAAAGAA GAAGAGCGAC 
CAGGCCCTGA ACTGCGGTGG GACTGCCTCG ACTGGCAGCG CGGGAAACGT CAAACTCGAG 
GAAAGTGCAG ATAACATACT CTCCATTGTT AAACAAAGAA CAGGATCTTT TGGGGATCGT 
CCTGCAAGAC CTACTCTTTT AGAACAAGTG TTAAATCAAA AAAGACTGTC GTTACTAAGA 
AGTCCAGAAG TAGTGCAATT TTTACAGAAA CAGCAACAGC TATTAAATCA GCAAGTTTTG 
GAGCAAAGAC AACAGCAGTT TCCAGGAACA TCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000529.1
CDS	157..975
Translation	MEAQGVAEGAGPGAASGVPHPAALAPAAAPTLAPASVAAAASQFTLLVMQPCAGQDEAAAPGGSVGAGKPVRYLCEGAGDGEEEAGEDEADLLDTSDPPGGGESAASLEDLEDEETHSGGEGSSGGARRRGSGGGSMSKTCTYEGCSETTSQVAKQRKPWMCKKHRNKMYKDKYKKKKSDQALNCGGTASTGSAGNVKLEESADNILSIVKQRTGSFGDRPARPTLLEQVLNQKRLSLLRSPEVVQFLQKQQQLLNQQVLEQRQQQFPGTSM

Target ORF information:

RefSeq Version	NM_000538.3
Organism	Homo sapiens(human)
Definition	Homo sapiens regulatory factor X associated protein (RFXAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000538.3

ORF Insert Sequence:

ATGGAGGCGC AGGGTGTAGC GGAGGGCGCG GGGCCGGGCG CCGCCAGCGG CGTGCCCCAC 
CCCGCGGCCC TAGCCCCGGC TGCGGCTCCC ACCTTGGCGC CAGCCTCGGT GGCGGCCGCG 
GCCTCTCAAT TCACCCTGCT AGTGATGCAA CCCTGTGCTG GGCAGGACGA GGCTGCGGCC 
CCCGGGGGCA GCGTTGGGGC GGGCAAGCCC GTTAGGTACC TGTGCGAAGG GGCCGGGGAT 
GGCGAAGAGG AGGCTGGGGA GGACGAGGCG GACCTGTTAG ACACTTCGGA CCCTCCGGGG 
GGAGGCGAGA GCGCGGCTAG TTTGGAGGAT CTAGAGGACG AGGAGACTCA CTCGGGGGGC 
GAGGGCAGCA GCGGGGGCGC CCGGAGGCGG GGCAGCGGTG GGGGCAGCAT GAGCAAGACC 
TGCACCTACG AAGGCTGCAG CGAGACCACG AGCCAGGTGG CCAAGCAGCG CAAACCGTGG 
ATGTGCAAGA AACACCGCAA CAAGATGTAC AAGGACAAGT ATAAAAAGAA GAAGAGCGAC 
CAGGCCCTGA ACTGCGGTGG GACTGCCTCG ACTGGCAGCG CGGGAAACGT CAAACTCGAG 
GAAAGTGCAG ATAACATACT CTCCATTGTT AAACAAAGAA CAGGATCTTT TGGGGATCGT 
CCTGCAAGAC CTACTCTTTT AGAACAAGTG TTAAATCAAA AAAGACTGTC GTTACTAAGA 
AGTCCAGAAG TAGTGCAATT TTTACAGAAA CAGCAACAGC TATTAAATCA GCAAGTTTTG 
GAGCAAAGAC AACAGCAGTT TCCAGGAACA TCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pancreatic cancer-derived exosomes transfer miRNAs to dendritic cells and inhibit RFXAP expression via miR-212-3p.
Oncotarget6(30)29877-88(2015 Oct)
Ding G,Zhou L,Qian Y,Fu M,Chen J,Chen J,Xiang J,Wu Z,Jiang G,Cao L

Solution structure of the heterotrimeric complex between the interaction domains of RFX5 and RFXAP from the RFX gene regulatory complex.
Journal of molecular biology403(1)40-51(2010 Oct)
Laird KM,Briggs LL,Boss JM,Summers MF,Garvie CW

Coordinate loss of MHC class II expression in the diffuse large B cell lymphoma cell line OCI-Ly2 is due to a novel mutation in RFX-AP.
Immunogenetics62(2)109-16(2010 Feb)
Bushway M,Cycon KA,Mulvaney K,Murphy SP

Formation of the RFX gene regulatory complex induces folding of the interaction domain of RFXAP.
Proteins76(3)655-64(2009 Aug)
Briggs L,Laird K,Boss JM,Garvie CW

Assembly of the RFX complex on the MHCII promoter: role of RFXAP and RFXB in relieving autoinhibition of RFX5.
Biochimica et biophysica acta1779(12)797-804(2008 Dec)
Garvie CW,Boss JM

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RFXAP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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