PRDM12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRDM12
Entrez Gene ID	59335
Full Name	PR/SET domain 12
Synonyms	HSAN8,PFM9
General protein information	Preferred Names PR/SET domain 12 Names PR domain zinc finger protein 12 PR domain containing 12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.12
Summary	This gene encodes a transcriptional regulator of sensory neuronal specification that plays a critical role in pain perception. The encoded protein contains an N-terminal PRDI-BF1 and RIZ homology (PR) domain, a SET domain, and three C-terminal C2H2 zinc finger DNA-binding domains. Naturally occurring mutations in this gene are associated with congenital insensitivity to pain (CIP), and hereditary sensory and autonomic neuropathies (HSAN's) affecting peripheral sensory and autonomic neurons. Deregulation of this gene is associated with solid cancers and hematological malignancies including chronic myeloid leukaemia. [provided by RefSeq, Mar 2017].
Disorder MIM:	616458
Disorder Html:	Neuropathy, hereditary sensory and autonomic, type VIII, 616488 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021619.2	NP_067632.2	PR domain zinc finger protein 12
NM_021619.3	NP_067632.2	PR domain zinc finger protein 12

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prdm12	NP_001116834.1
Rattus norvegicus (Norway rat)	Prdm12	XP_001067974.2
Canis lupus familiaris (dog)	LOC608616	XP_005625499.1
Bos taurus (cattle)	PRDM12	NP_001193224.1
Pan troglodytes (chimpanzee)	PRDM12	XP_528445.2
Xenopus tropicalis (tropical clawed frog)	prdm12	NP_001072898.1
Danio rerio (zebrafish)	prdm12b	NP_001007458.1
Homo sapiens (human)	PRDM12	NP_067632.2
Gallus gallus (chicken)	PRDM12	XP_415465.2

Gene Ontology
Bibliography

Related articles in PubMed

Hereditary Sensory Polyneuropathy, Pain Insensitivity and Global Developmental Delay due to Novel Mutation in PRDM12 Gene.
Saini AG, Padmanabh H, Sahu JK, Kurth I, Voigt M, Singhi P
Indian journal of pediatrics84(4)332-333(2017 04)

Clinical features for diagnosis and management of patients with PRDM12 congenital insensitivity to pain.
Zhang S, Malik Sharif S, Chen YC, Valente EM, Ahmed M, Sheridan E, Bennett C, Woods G
Journal of medical genetics53(8)533-5(2016 Aug)

Transcriptional regulator PRDM12 is essential for human pain perception.
Chen YC, Auer-Grumbach M, Matsukawa S, Zitzelsberger M, Themistocleous AC, Strom TM, Samara C, Moore AW, Cho LT, Young GT, Weiss C, Schabh?ttl M, Stucka R, Schmid AB, Parman Y, Graul-Neumann L, Heinritz W, Passarge E, Watson RM, Hertz JM, Moog U, Baumgartner M, Valente EM, Pereira D, Restrepo CM, Katona I, Dusl M, Stendel C, Wieland T, Stafford F, Reimann F, von Au K, Finke C, Willems PJ, Nahorski MS, Shaikh SS, Carvalho OP, Nicholas AK, Karbani G, McAleer MA, Cilio MR, McHugh JC, Murphy SM, Irvine AD, Jensen UB, Windhager R, Weis J, Bergmann C, Rautenstrauss B, Baets J, De Jonghe P, Reilly MM, Kropatsch R, Kurth I, Chrast R, Michiue T, Bennett DL, Woods CG, Senderek J
Nature genetics47(7)803-8(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The natural history of the PRDM12-CIP disorder.
Title: Clinical features for diagnosis and management of patients with PRDM12 congenital insensitivity to pain.

Prdm12 is a key factor in the orchestration of sensory neurogenesis.
Title: Transcriptional regulator PRDM12 is essential for human pain perception.

PRDM12 and its functional fly homolog Hamlet are evolutionary conserved master regulators of sensory neuronal specification and play a critical role in pain perception
Title: The evolutionarily conserved transcription factor PRDM12 controls sensory neuron development and pain perception.

Down-regulation of PRDM12 is associated with the pathogenesis of chronic myeloid leukaemia with derivative chromosome 9 deletion
Title: A potential role for PRDM12 in the pathogenesis of chronic myeloid leukaemia with derivative chromosome 9 deletion.

The following PRDM12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRDM12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31217	NM_021619.3 Latest version!	Homo sapiens PR/SET domain 12 (PRDM12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu31217	NM_021619.2	Homo sapiens PR/SET domain 12 (PRDM12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRDM12 ( NM_021619.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_067632.2 Homo sapiens PR/SET domain 12 (PRDM12), mRNA.

Price & Availability↑

CloneID	OHu31217
Clone ID Related Accession (Same CDS sequence)	NM_021619.2 , NM_021619.3
Accession Version	NM_021619.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	PR domain zinc finger protein 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY004252.2. This sequence is a reference standard in the RefSeqGene project. On Jul 31, 2001 this sequence version replaced NM_021619.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY004252.2 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGGCT CCGTGCTCCC GGCTGAGGCC CTGGTGCTCA AGACCGGGCT GAAGGCGCCG 
GGACTGGCGC TGGCCGAGGT TATCACCTCC GACATCCTGC ACAGCTTCCT GTACGGCCGC 
TGGCGCAACG TGCTCGGGGA GCAGCTCTTC GAGGACAAGA GCCACCACGC CAGCCCCAAG 
ACAGCCTTCA CCGCCGAGGT GCTGGCGCAG TCCTTCTCCG GCGAAGTGCA GAAGCTGTCC 
AGCCTGGTGC TGCCTGCGGA GGTGATCATC GCTCAGAGCT CCATCCCTGG CGAGGGCCTC 
GGCATCTTCT CCAAGACGTG GATCAAGGCG GGAACCGAGA TGGGCCCCTT CACCGGCCGC 
GTGATCGCCC CGGAGCACGT GGACATCTGC AAGAACAACA ACCTCATGTG GGAGGTGTTC 
AATGAGGATG GCACGGTGCG CTACTTCATC GATGCCAGCC AGGAGGACCA CCGGAGCTGG 
ATGACCTACA TCAAGTGTGC ACGTAACGAA CAGGAGCAGA ACCTGGAGGT GGTCCAGATC 
GGCACCAGCA TCTTCTACAA GGCCATTGAG ATGATCCCAC CTGACCAGGA ACTGCTGGTG 
TGGTACGGAA ACTCACACAA CACCTTCCTG GGGATCCCAG GTGTGCCCGG GCTAGAGGAG 
GACCAGAAAA AGAACAAGCA TGAGGACTTC CACCCGGCGG ACTCGGCGGC TGGCCCCGCG 
GGCCGCATGC GATGCGTCAT CTGCCACCGC GGCTTCAACT CGCGCAGCAA CCTGCGCTCG 
CACATGCGCA TCCACACGCT GGACAAGCCC TTCGTGTGCC GCTTCTGCAA CCGCCGCTTC 
AGCCAGTCGT CCACGCTGCG CAACCACGTG CGCCTGCACA CGGGCGAGCG CCCCTACAAG 
TGCCAGGTGT GCCAGAGCGC CTACTCGCAG CTGGCCGGCC TGCGCGCCCA CCAGAAGAGC 
GCGCGGCACC GGCCGCCCAG CACCGCGCTG CAGGCACACT CGCCCGCGCT GCCCGCCCCG 
CACGCGCACG CGCCCGCGCT CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCGCAC 
CACCTGCCGG CCATGGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067632.2
CDS	61..1164
Translation	MMGSVLPAEALVLKTGLKAPGLALAEVITSDILHSFLYGRWRNVLGEQLFEDKSHHASPKTAFTAEVLAQSFSGEVQKLSSLVLPAEVIIAQSSIPGEGLGIFSKTWIKAGTEMGPFTGRVIAPEHVDICKNNNLMWEVFNEDGTVRYFIDASQEDHRSWMTYIKCARNEQEQNLEVVQIGTSIFYKAIEMIPPDQELLVWYGNSHNTFLGIPGVPGLEEDQKKNKHEDFHPADSAAGPAGRMRCVICHRGFNSRSNLRSHMRIHTLDKPFVCRFCNRRFSQSSTLRNHVRLHTGERPYKCQVCQSAYSQLAGLRAHQKSARHRPPSTALQAHSPALPAPHAHAPALAAAAAAAAAAAAHHLPAMVL

Target ORF information:

RefSeq Version	NM_021619.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 12 (PRDM12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021619.2

ORF Insert Sequence:

ATGATGGGCT CCGTGCTCCC GGCTGAGGCC CTGGTGCTCA AGACCGGGCT GAAGGCGCCG 
GGACTGGCGC TGGCCGAGGT TATCACCTCC GACATCCTGC ACAGCTTCCT GTACGGCCGC 
TGGCGCAACG TGCTCGGGGA GCAGCTCTTC GAGGACAAGA GCCACCACGC CAGCCCCAAG 
ACAGCCTTCA CCGCCGAGGT GCTGGCGCAG TCCTTCTCCG GCGAAGTGCA GAAGCTGTCC 
AGCCTGGTGC TGCCTGCGGA GGTGATCATC GCTCAGAGCT CCATCCCTGG CGAGGGCCTC 
GGCATCTTCT CCAAGACGTG GATCAAGGCG GGAACCGAGA TGGGCCCCTT CACCGGCCGC 
GTGATCGCCC CGGAGCACGT GGACATCTGC AAGAACAACA ACCTCATGTG GGAGGTGTTC 
AATGAGGATG GCACGGTGCG CTACTTCATC GATGCCAGCC AGGAGGACCA CCGGAGCTGG 
ATGACCTACA TCAAGTGTGC ACGTAACGAA CAGGAGCAGA ACCTGGAGGT GGTCCAGATC 
GGCACCAGCA TCTTCTACAA GGCCATTGAG ATGATCCCAC CTGACCAGGA ACTGCTGGTG 
TGGTACGGAA ACTCACACAA CACCTTCCTG GGGATCCCAG GTGTGCCCGG GCTAGAGGAG 
GACCAGAAAA AGAACAAGCA TGAGGACTTC CACCCGGCGG ACTCGGCGGC TGGCCCCGCG 
GGCCGCATGC GATGCGTCAT CTGCCACCGC GGCTTCAACT CGCGCAGCAA CCTGCGCTCG 
CACATGCGCA TCCACACGCT GGACAAGCCC TTCGTGTGCC GCTTCTGCAA CCGCCGCTTC 
AGCCAGTCGT CCACGCTGCG CAACCACGTG CGCCTGCACA CGGGCGAGCG CCCCTACAAG 
TGCCAGGTGT GCCAGAGCGC CTACTCGCAG CTGGCCGGCC TGCGCGCCCA CCAGAAGAGC 
GCGCGGCACC GGCCGCCCAG CACCGCGCTG CAGGCACACT CGCCCGCGCT GCCCGCCCCG 
CACGCGCACG CGCCCGCGCT CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCGCAC 
CACCTGCCGG CCATGGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRDM12 ( NM_021619.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_067632.2 Homo sapiens PR/SET domain 12 (PRDM12), mRNA.

Price & Availability↑

CloneID	OHu31217
Clone ID Related Accession (Same CDS sequence)	NM_021619.2 , NM_021619.3
Accession Version	NM_021619.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	PR domain zinc finger protein 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY004252.2. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_021619.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY004252.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253008.3/ ENSP00000253008.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGGGCT CCGTGCTCCC GGCTGAGGCC CTGGTGCTCA AGACCGGGCT GAAGGCGCCG 
GGACTGGCGC TGGCCGAGGT TATCACCTCC GACATCCTGC ACAGCTTCCT GTACGGCCGC 
TGGCGCAACG TGCTCGGGGA GCAGCTCTTC GAGGACAAGA GCCACCACGC CAGCCCCAAG 
ACAGCCTTCA CCGCCGAGGT GCTGGCGCAG TCCTTCTCCG GCGAAGTGCA GAAGCTGTCC 
AGCCTGGTGC TGCCTGCGGA GGTGATCATC GCTCAGAGCT CCATCCCTGG CGAGGGCCTC 
GGCATCTTCT CCAAGACGTG GATCAAGGCG GGAACCGAGA TGGGCCCCTT CACCGGCCGC 
GTGATCGCCC CGGAGCACGT GGACATCTGC AAGAACAACA ACCTCATGTG GGAGGTGTTC 
AATGAGGATG GCACGGTGCG CTACTTCATC GATGCCAGCC AGGAGGACCA CCGGAGCTGG 
ATGACCTACA TCAAGTGTGC ACGTAACGAA CAGGAGCAGA ACCTGGAGGT GGTCCAGATC 
GGCACCAGCA TCTTCTACAA GGCCATTGAG ATGATCCCAC CTGACCAGGA ACTGCTGGTG 
TGGTACGGAA ACTCACACAA CACCTTCCTG GGGATCCCAG GTGTGCCCGG GCTAGAGGAG 
GACCAGAAAA AGAACAAGCA TGAGGACTTC CACCCGGCGG ACTCGGCGGC TGGCCCCGCG 
GGCCGCATGC GATGCGTCAT CTGCCACCGC GGCTTCAACT CGCGCAGCAA CCTGCGCTCG 
CACATGCGCA TCCACACGCT GGACAAGCCC TTCGTGTGCC GCTTCTGCAA CCGCCGCTTC 
AGCCAGTCGT CCACGCTGCG CAACCACGTG CGCCTGCACA CGGGCGAGCG CCCCTACAAG 
TGCCAGGTGT GCCAGAGCGC CTACTCGCAG CTGGCCGGCC TGCGCGCCCA CCAGAAGAGC 
GCGCGGCACC GGCCGCCCAG CACCGCGCTG CAGGCACACT CGCCCGCGCT GCCCGCCCCG 
CACGCGCACG CGCCCGCGCT CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCGCAC 
CACCTGCCGG CCATGGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067632.2
CDS	61..1164
Translation	MMGSVLPAEALVLKTGLKAPGLALAEVITSDILHSFLYGRWRNVLGEQLFEDKSHHASPKTAFTAEVLAQSFSGEVQKLSSLVLPAEVIIAQSSIPGEGLGIFSKTWIKAGTEMGPFTGRVIAPEHVDICKNNNLMWEVFNEDGTVRYFIDASQEDHRSWMTYIKCARNEQEQNLEVVQIGTSIFYKAIEMIPPDQELLVWYGNSHNTFLGIPGVPGLEEDQKKNKHEDFHPADSAAGPAGRMRCVICHRGFNSRSNLRSHMRIHTLDKPFVCRFCNRRFSQSSTLRNHVRLHTGERPYKCQVCQSAYSQLAGLRAHQKSARHRPPSTALQAHSPALPAPHAHAPALAAAAAAAAAAAAHHLPAMVL

Target ORF information:

RefSeq Version	NM_021619.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 12 (PRDM12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021619.3

ORF Insert Sequence:

ATGATGGGCT CCGTGCTCCC GGCTGAGGCC CTGGTGCTCA AGACCGGGCT GAAGGCGCCG 
GGACTGGCGC TGGCCGAGGT TATCACCTCC GACATCCTGC ACAGCTTCCT GTACGGCCGC 
TGGCGCAACG TGCTCGGGGA GCAGCTCTTC GAGGACAAGA GCCACCACGC CAGCCCCAAG 
ACAGCCTTCA CCGCCGAGGT GCTGGCGCAG TCCTTCTCCG GCGAAGTGCA GAAGCTGTCC 
AGCCTGGTGC TGCCTGCGGA GGTGATCATC GCTCAGAGCT CCATCCCTGG CGAGGGCCTC 
GGCATCTTCT CCAAGACGTG GATCAAGGCG GGAACCGAGA TGGGCCCCTT CACCGGCCGC 
GTGATCGCCC CGGAGCACGT GGACATCTGC AAGAACAACA ACCTCATGTG GGAGGTGTTC 
AATGAGGATG GCACGGTGCG CTACTTCATC GATGCCAGCC AGGAGGACCA CCGGAGCTGG 
ATGACCTACA TCAAGTGTGC ACGTAACGAA CAGGAGCAGA ACCTGGAGGT GGTCCAGATC 
GGCACCAGCA TCTTCTACAA GGCCATTGAG ATGATCCCAC CTGACCAGGA ACTGCTGGTG 
TGGTACGGAA ACTCACACAA CACCTTCCTG GGGATCCCAG GTGTGCCCGG GCTAGAGGAG 
GACCAGAAAA AGAACAAGCA TGAGGACTTC CACCCGGCGG ACTCGGCGGC TGGCCCCGCG 
GGCCGCATGC GATGCGTCAT CTGCCACCGC GGCTTCAACT CGCGCAGCAA CCTGCGCTCG 
CACATGCGCA TCCACACGCT GGACAAGCCC TTCGTGTGCC GCTTCTGCAA CCGCCGCTTC 
AGCCAGTCGT CCACGCTGCG CAACCACGTG CGCCTGCACA CGGGCGAGCG CCCCTACAAG 
TGCCAGGTGT GCCAGAGCGC CTACTCGCAG CTGGCCGGCC TGCGCGCCCA CCAGAAGAGC 
GCGCGGCACC GGCCGCCCAG CACCGCGCTG CAGGCACACT CGCCCGCGCT GCCCGCCCCG 
CACGCGCACG CGCCCGCGCT CGCCGCCGCC GCCGCCGCCG CCGCCGCCGC CGCCGCGCAC 
CACCTGCCGG CCATGGTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hereditary Sensory Polyneuropathy, Pain Insensitivity and Global Developmental Delay due to Novel Mutation in PRDM12 Gene.
Indian journal of pediatrics84(4)332-333(2017 04)
Saini AG,Padmanabh H,Sahu JK,Kurth I,Voigt M,Singhi P

Clinical features for diagnosis and management of patients with PRDM12 congenital insensitivity to pain.
Journal of medical genetics53(8)533-5(2016 Aug)
Zhang S,Malik Sharif S,Chen YC,Valente EM,Ahmed M,Sheridan E,Bennett C,Woods G

Transcriptional regulator PRDM12 is essential for human pain perception.
Nature genetics47(7)803-8(2015 Jul)
Chen YC,Auer-Grumbach M,Matsukawa S,Zitzelsberger M,Themistocleous AC,Strom TM,Samara C,Moore AW,Cho LT,Young GT,Weiss C,Schabh?ttl M,Stucka R,Schmid AB,Parman Y,Graul-Neumann L,Heinritz W,Passarge E,Watson RM,Hertz JM,Moog U,Baumgartner M,Valente EM,Pereira D,Restrepo CM,Katona I,Dusl M,Stendel C,Wieland T,Stafford F,Reimann F,von Au K,Finke C,Willems PJ,Nahorski MS,Shaikh SS,Carvalho OP,Nicholas AK,Karbani G,McAleer MA,Cilio MR,McHugh JC,Murphy SM,Irvine AD,Jensen UB,Windhager R,Weis J,Bergmann C,Rautenstrauss B,Baets J,De Jonghe P,Reilly MM,Kropatsch R,Kurth I,Chrast R,Michiue T,Bennett DL,Woods CG,Senderek J

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PRDM12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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