ORF » Species Summary » Homo sapiens » BCKDHA cDNA ORF clone

BCKDHA cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following BCKDHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCKDHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24779 NM_000709.4
Latest version!		 Homo sapiens branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu24785 NM_001164783.2
Latest version!		 Homo sapiens branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
View Old Accession Versions >>
OHu24779 NM_000709.3 Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00
OHu24785 NM_001164783.1 Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    BCKDHA ( NM_000709.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000700.1 Homo sapiens branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu24779
    Clone ID Related Accession (Same CDS sequence) NM_000709.4 , NM_000709.3
    Accession Version NM_000709.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-09
    Organism Homo sapiens(human)
    Product 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC023983.1, AK298188.1 and BQ018849.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000709.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC023983.1, SRR3476690.198658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000269980.7/ ENSP00000269980.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCAGCACG AGGCCCCGGG TATGGCATCA TGTCAATCCG CGTGGATGGT 
    AATGATGTGT TTGCCGTATA CAACGCCACA AAGGAGGCCC GACGGCGGGC TGTGGCAGAG 
    AACCAGCCCT TCCTCATCGA GGCCATGACC TACAGGATCG GGCACCACAG CACCAGTGAC 
    GACAGTTCAG CGTACCGCTC GGTGGATGAG GTCAATTACT GGGATAAACA GGACCACCCC 
    ATCTCCCGGC TGCGGCACTA TCTGCTGAGC CAAGGCTGGT GGGATGAGGA GCAGGAGAAG 
    GCCTGGAGGA AGCAGTCCCG CAGGAAGGTG ATGGAGGCCT TTGAGCAGGC CGAGCGGAAG 
    CCCAAACCCA ACCCCAACCT ACTCTTCTCA GACGTGTATC AGGAGATGCC CGCCCAGCTC 
    CGCAAGCAGC AGGAGTCTCT GGCCCGCCAC CTGCAGACCT ACGGGGAGCA CTACCCACTG 
    GATCACTTCG ATAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000700.1
    CDS11..1348
    Translation

    Target ORF information:

    RefSeq Version NM_000709.4
    Organism Homo sapiens(human)
    Definition Homo sapiens branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000709.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCAGCACG AGGCCCCGGG TATGGCATCA TGTCAATCCG CGTGGATGGT 
    AATGATGTGT TTGCCGTATA CAACGCCACA AAGGAGGCCC GACGGCGGGC TGTGGCAGAG 
    AACCAGCCCT TCCTCATCGA GGCCATGACC TACAGGATCG GGCACCACAG CACCAGTGAC 
    GACAGTTCAG CGTACCGCTC GGTGGATGAG GTCAATTACT GGGATAAACA GGACCACCCC 
    ATCTCCCGGC TGCGGCACTA TCTGCTGAGC CAAGGCTGGT GGGATGAGGA GCAGGAGAAG 
    GCCTGGAGGA AGCAGTCCCG CAGGAAGGTG ATGGAGGCCT TTGAGCAGGC CGAGCGGAAG 
    CCCAAACCCA ACCCCAACCT ACTCTTCTCA GACGTGTATC AGGAGATGCC CGCCCAGCTC 
    CGCAAGCAGC AGGAGTCTCT GGCCCGCCAC CTGCAGACCT ACGGGGAGCA CTACCCACTG 
    GATCACTTCG ATAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BCKDHA ( NM_000709.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000700.1 Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu24779
    Clone ID Related Accession (Same CDS sequence) NM_000709.4 , NM_000709.3
    Accession Version NM_000709.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI910860.1, BC023983.1, AK298188.1 and BQ018849.1. This sequence is a reference standard in the RefSeqGene project. On Sep 18, 2009 this sequence version replaced NM_000709.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.304912.1, SRR1163657.232047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCAGCACG AGGCCCCGGG TATGGCATCA TGTCAATCCG CGTGGATGGT 
    AATGATGTGT TTGCCGTATA CAACGCCACA AAGGAGGCCC GACGGCGGGC TGTGGCAGAG 
    AACCAGCCCT TCCTCATCGA GGCCATGACC TACAGGATCG GGCACCACAG CACCAGTGAC 
    GACAGTTCAG CGTACCGCTC GGTGGATGAG GTCAATTACT GGGATAAACA GGACCACCCC 
    ATCTCCCGGC TGCGGCACTA TCTGCTGAGC CAAGGCTGGT GGGATGAGGA GCAGGAGAAG 
    GCCTGGAGGA AGCAGTCCCG CAGGAAGGTG ATGGAGGCCT TTGAGCAGGC CGAGCGGAAG 
    CCCAAACCCA ACCCCAACCT ACTCTTCTCA GACGTGTATC AGGAGATGCC CGCCCAGCTC 
    CGCAAGCAGC AGGAGTCTCT GGCCCGCCAC CTGCAGACCT ACGGGGAGCA CTACCCACTG 
    GATCACTTCG ATAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000700.1
    CDS40..1377
    Translation

    Target ORF information:

    RefSeq Version NM_000709.3
    Organism Homo sapiens(human)
    Definition Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000709.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCAGCACG AGGCCCCGGG TATGGCATCA TGTCAATCCG CGTGGATGGT 
    AATGATGTGT TTGCCGTATA CAACGCCACA AAGGAGGCCC GACGGCGGGC TGTGGCAGAG 
    AACCAGCCCT TCCTCATCGA GGCCATGACC TACAGGATCG GGCACCACAG CACCAGTGAC 
    GACAGTTCAG CGTACCGCTC GGTGGATGAG GTCAATTACT GGGATAAACA GGACCACCCC 
    ATCTCCCGGC TGCGGCACTA TCTGCTGAGC CAAGGCTGGT GGGATGAGGA GCAGGAGAAG 
    GCCTGGAGGA AGCAGTCCCG CAGGAAGGTG ATGGAGGCCT TTGAGCAGGC CGAGCGGAAG 
    CCCAAACCCA ACCCCAACCT ACTCTTCTCA GACGTGTATC AGGAGATGCC CGCCCAGCTC 
    CGCAAGCAGC AGGAGTCTCT GGCCCGCCAC CTGCAGACCT ACGGGGAGCA CTACCCACTG 
    GATCACTTCG ATAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BCKDHA ( NM_001164783.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158255.1 Homo sapiens branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu24785
    Clone ID Related Accession (Same CDS sequence) NM_001164783.2 , NM_001164783.1
    Accession Version NM_001164783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI910860.1, AB209828.1, AK298188.1 and BQ018849.1. On May 31, 2019 this sequence version replaced NM_001164783.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks one internal amino acid, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.262820.1, SRR1803617.198818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCACGAGG CCCCGGGTAT GGCATCATGT CAATCCGCGT GGATGGTAAT 
    GATGTGTTTG CCGTATACAA CGCCACAAAG GAGGCCCGAC GGCGGGCTGT GGCAGAGAAC 
    CAGCCCTTCC TCATCGAGGC CATGACCTAC AGGATCGGGC ACCACAGCAC CAGTGACGAC 
    AGTTCAGCGT ACCGCTCGGT GGATGAGGTC AATTACTGGG ATAAACAGGA CCACCCCATC 
    TCCCGGCTGC GGCACTATCT GCTGAGCCAA GGCTGGTGGG ATGAGGAGCA GGAGAAGGCC 
    TGGAGGAAGC AGTCCCGCAG GAAGGTGATG GAGGCCTTTG AGCAGGCCGA GCGGAAGCCC 
    AAACCCAACC CCAACCTACT CTTCTCAGAC GTGTATCAGG AGATGCCCGC CCAGCTCCGC 
    AAGCAGCAGG AGTCTCTGGC CCGCCACCTG CAGACCTACG GGGAGCACTA CCCACTGGAT 
    CACTTCGATA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001158255.1
    CDS11..1345
    Translation

    Target ORF information:

    RefSeq Version NM_001164783.2
    Organism Homo sapiens(human)
    Definition Homo sapiens branched chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001164783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCACGAGG CCCCGGGTAT GGCATCATGT CAATCCGCGT GGATGGTAAT 
    GATGTGTTTG CCGTATACAA CGCCACAAAG GAGGCCCGAC GGCGGGCTGT GGCAGAGAAC 
    CAGCCCTTCC TCATCGAGGC CATGACCTAC AGGATCGGGC ACCACAGCAC CAGTGACGAC 
    AGTTCAGCGT ACCGCTCGGT GGATGAGGTC AATTACTGGG ATAAACAGGA CCACCCCATC 
    TCCCGGCTGC GGCACTATCT GCTGAGCCAA GGCTGGTGGG ATGAGGAGCA GGAGAAGGCC 
    TGGAGGAAGC AGTCCCGCAG GAAGGTGATG GAGGCCTTTG AGCAGGCCGA GCGGAAGCCC 
    AAACCCAACC CCAACCTACT CTTCTCAGAC GTGTATCAGG AGATGCCCGC CCAGCTCCGC 
    AAGCAGCAGG AGTCTCTGGC CCGCCACCTG CAGACCTACG GGGAGCACTA CCCACTGGAT 
    CACTTCGATA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    BCKDHA ( NM_001164783.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158255.1 Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu24785
    Clone ID Related Accession (Same CDS sequence) NM_001164783.2 , NM_001164783.1
    Accession Version NM_001164783.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI910860.1, AB209828.1, AK298188.1 and BQ018849.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks one internal amino acid, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.262820.1, AB209828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCACGAGG CCCCGGGTAT GGCATCATGT CAATCCGCGT GGATGGTAAT 
    GATGTGTTTG CCGTATACAA CGCCACAAAG GAGGCCCGAC GGCGGGCTGT GGCAGAGAAC 
    CAGCCCTTCC TCATCGAGGC CATGACCTAC AGGATCGGGC ACCACAGCAC CAGTGACGAC 
    AGTTCAGCGT ACCGCTCGGT GGATGAGGTC AATTACTGGG ATAAACAGGA CCACCCCATC 
    TCCCGGCTGC GGCACTATCT GCTGAGCCAA GGCTGGTGGG ATGAGGAGCA GGAGAAGGCC 
    TGGAGGAAGC AGTCCCGCAG GAAGGTGATG GAGGCCTTTG AGCAGGCCGA GCGGAAGCCC 
    AAACCCAACC CCAACCTACT CTTCTCAGAC GTGTATCAGG AGATGCCCGC CCAGCTCCGC 
    AAGCAGCAGG AGTCTCTGGC CCGCCACCTG CAGACCTACG GGGAGCACTA CCCACTGGAT 
    CACTTCGATA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001158255.1
    CDS40..1374
    Translation

    Target ORF information:

    RefSeq Version NM_001164783.1
    Organism Homo sapiens(human)
    Definition Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001164783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCGGTAG CGATCGCTGC AGCGAGGGTC TGGCGGCTAA ACCGTGGTTT GAGCCAGGCT 
    GCCCTCCTGC TGCTGCGGCA GCCTGGGGCT CGGGGACTGG CTAGATCTCA CCCCCCCAGG 
    CAGCAGCAGC AGTTTTCATC TCTGGATGAC AAGCCCCAGT TCCCAGGGGC CTCGGCGGAG 
    TTTATAGATA AGTTGGAATT CATCCAGCCC AACGTCATCT CTGGAATCCC CATCTACCGC 
    GTCATGGACC GGCAAGGCCA GATCATCAAC CCCAGCGAGG ACCCCCACCT GCCGAAGGAG 
    AAGGTGCTGA AGCTCTACAA GAGCATGACA CTGCTTAACA CCATGGACCG CATCCTCTAT 
    GAGTCTCAGC GGCAGGGCCG GATCTCCTTC TACATGACCA ACTATGGTGA GGAGGGCACG 
    CACGTGGGGA GTGCCGCCGC CCTGGACAAC ACGGACCTGG TGTTTGGCCA GTACCGGGAG 
    GCAGGTGTGC TGATGTATCG GGACTACCCC CTGGAACTAT TCATGGCCCA GTGCTATGGC 
    AACATCAGTG ACTTGGGCAA GGGGCGCCAG ATGCCTGTCC ACTACGGCTG CAAGGAACGC 
    CACTTCGTCA CTATCTCCTC TCCACTGGCC ACGCAGATCC CTCAGGCGGT GGGGGCGGCG 
    TACGCAGCCA AGCGGGCCAA TGCCAACAGG GTCGTCATCT GTTACTTCGG CGAGGGGGCA 
    GCCAGTGAGG GGGACGCCCA TGCCGGCTTC AACTTCGCTG CCACACTTGA GTGCCCCATC 
    ATCTTCTTCT GCCGGAACAA TGGCTACGCC ATCTCCACGC CCACCTCTGA GCAGTATCGC 
    GGCGATGGCA TTGCACGAGG CCCCGGGTAT GGCATCATGT CAATCCGCGT GGATGGTAAT 
    GATGTGTTTG CCGTATACAA CGCCACAAAG GAGGCCCGAC GGCGGGCTGT GGCAGAGAAC 
    CAGCCCTTCC TCATCGAGGC CATGACCTAC AGGATCGGGC ACCACAGCAC CAGTGACGAC 
    AGTTCAGCGT ACCGCTCGGT GGATGAGGTC AATTACTGGG ATAAACAGGA CCACCCCATC 
    TCCCGGCTGC GGCACTATCT GCTGAGCCAA GGCTGGTGGG ATGAGGAGCA GGAGAAGGCC 
    TGGAGGAAGC AGTCCCGCAG GAAGGTGATG GAGGCCTTTG AGCAGGCCGA GCGGAAGCCC 
    AAACCCAACC CCAACCTACT CTTCTCAGAC GTGTATCAGG AGATGCCCGC CCAGCTCCGC 
    AAGCAGCAGG AGTCTCTGGC CCGCCACCTG CAGACCTACG GGGAGCACTA CCCACTGGAT 
    CACTTCGATA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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