RAD9A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD9A
Entrez Gene ID	5883
Full Name	RAD9 checkpoint clamp component A
Synonyms	RAD9
General protein information	Preferred Names RAD9 checkpoint clamp component A Names cell cycle checkpoint control protein RAD9A DNA repair exonuclease rad9 homolog A RAD9 homolog A hRAD9
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.2
Summary	This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:	603761

mRNA and Protein(s)

mRNA	Protein	Name
NM_004584.2	NP_004575.1	cell cycle checkpoint control protein RAD9A isoform 1
XM_006718652.3	XP_006718715.1	cell cycle checkpoint control protein RAD9A isoform X1
XM_017018097.1	XP_016873586.1	cell cycle checkpoint control protein RAD9A isoform X2
NM_004584.3	NP_004575.1	cell cycle checkpoint control protein RAD9A isoform 1
NM_001243224.1	NP_001230153.1	cell cycle checkpoint control protein RAD9A isoform 2
XM_017018098.1	XP_016873587.1	cell cycle checkpoint control protein RAD9A isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD9A	NP_004575.1
Bos taurus (cattle)	RAD9A	NP_001014848.1
Mus musculus (house mouse)	Rad9a	NP_035367.1
Rattus norvegicus (Norway rat)	Rad9a	XP_002728900.1
	RAD9A	XP_001106622.1
Canis lupus familiaris (dog)	RAD9A	XP_540817.3
Drosophila melanogaster (fruit fly)	Rad9	NP_001097639.2
Xenopus tropicalis (tropical clawed frog)	rad9a	NP_001005810.1
Gallus gallus (chicken)	RAD9A	NP_998748.1
Magnaporthe oryzae (rice blast fungus)	MGG_05278	XP_003712837.1

Gene Ontology
Bibliography

Related articles in PubMed

The CDK-PLK1 axis targets the DNA damage checkpoint sensor protein RAD9 to promote cell proliferation and tolerance to genotoxic stress.
Wakida T, Ikura M, Kuriya K, Ito S, Shiroiwa Y, Habu T, Kawamoto T, Okumura K, Ikura T, Furuya K
eLife6(2017 Dec)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint.
Awate S, De Benedetti A
BMC molecular biology173(2016 Feb)

Regulation of ATRIP protein abundance by RAD9 in the DNA damage repair pathway.
Peng XJ, Liu SJ, Bao CM, Liu YZ, Xie HW, Cai YH, Li BM, Hang HY, Ding X
Cellular and molecular biology (Noisy-le-Grand, France)61(8)31-6(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint
Title: TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint.

RAD9 has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling.
Title: Regulation of ATRIP protein abundance by RAD9 in the DNA damage repair pathway.

Intramolecular binding of the rad9 C-terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding.
Title: Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.

these results demonstrate a positive feedback loop involving Rad9A-dependend activation of Chk1.
Title: Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling.

The role of Rad9 in homologous recombination is independent of its function in checkpoint activation, and this function is important for preventing alternative non-homologous end joining.
Title: The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.

The following RAD9A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD9A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02206	NM_001243224.1 Latest version!	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu04471	NM_004584.3 Latest version!	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu60166	XM_017018098.1 Latest version!	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu45452	XM_006718652.3 Latest version!	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu92413	XM_017018097.1 Latest version!	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu04471	NM_004584.2	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD9A ( NM_001243224.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230153.1 Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02206
Clone ID Related Accession (Same CDS sequence)	NM_001243224.1
Accession Version	NM_001243224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	cell cycle checkpoint control protein RAD9A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB164642.1, CN402466.1, U53174.1 and AA708970.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon (with an in-frame AUG) compared to variant 1. This results in a a shorter isoform (2) with a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCTT TCCTGTCTGT CTTCCGCTCA CTGGCGATGC TGGAGAAGAC GGTGGAAAAA 
TGCTGCATCT CCCTGAATGG CCGGAGCAGC CGCCTGGTGG TCCAGCTGCA TTGCAAGTTC 
GGGGTGCGGA AGACTCACAA CCTGTCCTTC CAGGACTGTG AGTCCCTGCA GGCCGTCTTC 
GACCCAGCCT CGTGCCCCCA CATGCTCCGC GCCCCAGCAC GGGTTCTGGG GGAGGCTGTT 
CTGCCCTTCT CTCCTGCACT GGCTGAAGTG ACGCTGGGCA TTGGCCGTGG CCGCAGGGTC 
ATCCTGCGCA GCTACCACGA GGAGGAGGCA GACAGCACTG CCAAAGCCAT GGTGACTGAG 
ATGTGCCTTG GAGAGGAGGA TTTCCAGCAG CTGCAGGCCC AGGAAGGGGT GGCCATCACT 
TTCTGCCTCA AGGAATTCCG GGGGCTCCTG AGCTTTGCAG AGTCAGCAAA CTTGAATCTT 
AGCATTCATT TTGATGCTCC AGGCAGGCCC GCCATCTTCA CCATCAAGGA CTCTTTGCTG 
GACGGCCACT TTGTCTTGGC CACACTCTCA GACACCGACT CGCACTCCCA GGACCTGGGC 
TCCCCAGAGC GTCACCAGCC AGTGCCTCAG CTCCAGGCTC ACAGCACACC CCACCCGGAC 
GACTTTGCCA ATGACGACAT TGACTCTTAC ATGATCGCCA TGGAAACCAC TATAGGCAAT 
GAGGGCTCGC GGGTGCTGCC CTCCATTTCC CTTTCACCTG GCCCCCAGCC CCCCAAGAGC 
CCCGGTCCCC ACTCCGAGGA GGAAGATGAG GCTGAGCCCA GTACAGTGCC TGGGACTCCC 
CCACCCAAGA AGTTCCGCTC ACTGTTCTTC GGCTCCATCC TGGCCCCTGT ACGCTCCCCC 
CAGGGCCCCA GCCCTGTGCT GGCGGAAGAC AGTGAGGGTG AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230153.1
CDS	186..1133
Translation	MQSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

Target ORF information:

RefSeq Version	NM_001243224.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243224.1

ORF Insert Sequence:

ATGCAGTCTT TCCTGTCTGT CTTCCGCTCA CTGGCGATGC TGGAGAAGAC GGTGGAAAAA 
TGCTGCATCT CCCTGAATGG CCGGAGCAGC CGCCTGGTGG TCCAGCTGCA TTGCAAGTTC 
GGGGTGCGGA AGACTCACAA CCTGTCCTTC CAGGACTGTG AGTCCCTGCA GGCCGTCTTC 
GACCCAGCCT CGTGCCCCCA CATGCTCCGC GCCCCAGCAC GGGTTCTGGG GGAGGCTGTT 
CTGCCCTTCT CTCCTGCACT GGCTGAAGTG ACGCTGGGCA TTGGCCGTGG CCGCAGGGTC 
ATCCTGCGCA GCTACCACGA GGAGGAGGCA GACAGCACTG CCAAAGCCAT GGTGACTGAG 
ATGTGCCTTG GAGAGGAGGA TTTCCAGCAG CTGCAGGCCC AGGAAGGGGT GGCCATCACT 
TTCTGCCTCA AGGAATTCCG GGGGCTCCTG AGCTTTGCAG AGTCAGCAAA CTTGAATCTT 
AGCATTCATT TTGATGCTCC AGGCAGGCCC GCCATCTTCA CCATCAAGGA CTCTTTGCTG 
GACGGCCACT TTGTCTTGGC CACACTCTCA GACACCGACT CGCACTCCCA GGACCTGGGC 
TCCCCAGAGC GTCACCAGCC AGTGCCTCAG CTCCAGGCTC ACAGCACACC CCACCCGGAC 
GACTTTGCCA ATGACGACAT TGACTCTTAC ATGATCGCCA TGGAAACCAC TATAGGCAAT 
GAGGGCTCGC GGGTGCTGCC CTCCATTTCC CTTTCACCTG GCCCCCAGCC CCCCAAGAGC 
CCCGGTCCCC ACTCCGAGGA GGAAGATGAG GCTGAGCCCA GTACAGTGCC TGGGACTCCC 
CCACCCAAGA AGTTCCGCTC ACTGTTCTTC GGCTCCATCC TGGCCCCTGT ACGCTCCCCC 
CAGGGCCCCA GCCCTGTGCT GGCGGAAGAC AGTGAGGGTG AAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD9A ( NM_004584.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004575.1 Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04471
Clone ID Related Accession (Same CDS sequence)	NM_004584.2 , NM_004584.3
Accession Version	NM_004584.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	cell cycle checkpoint control protein RAD9A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014848.1, U53174.1 and AA708970.1. On Apr 4, 2002 this sequence version replaced NM_004584.1. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014848.1, U53174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTGCC TGGTCACGGG CGGCAACGTG AAGGTGCTCG GCAAGGCCGT CCACTCCCTG 
TCCCGCATCG GGGACGAGCT CTACCTGGAA CCCTTGGAGG ACGGGCTCTC CCTCCGGACG 
GTGAACTCCT CCCGCTCTGC CTATGCCTGC TTTCTCTTTG CCCCGCTCTT CTTCCAGCAA 
TACCAGGCAG CCACCCCTGG TCAGGACCTG CTGCGCTGTA AGATCCTGAT GAAGTCTTTC 
CTGTCTGTCT TCCGCTCACT GGCGATGCTG GAGAAGACGG TGGAAAAATG CTGCATCTCC 
CTGAATGGCC GGAGCAGCCG CCTGGTGGTC CAGCTGCATT GCAAGTTCGG GGTGCGGAAG 
ACTCACAACC TGTCCTTCCA GGACTGTGAG TCCCTGCAGG CCGTCTTCGA CCCAGCCTCG 
TGCCCCCACA TGCTCCGCGC CCCAGCACGG GTTCTGGGGG AGGCTGTTCT GCCCTTCTCT 
CCTGCACTGG CTGAAGTGAC GCTGGGCATT GGCCGTGGCC GCAGGGTCAT CCTGCGCAGC 
TACCACGAGG AGGAGGCAGA CAGCACTGCC AAAGCCATGG TGACTGAGAT GTGCCTTGGA 
GAGGAGGATT TCCAGCAGCT GCAGGCCCAG GAAGGGGTGG CCATCACTTT CTGCCTCAAG 
GAATTCCGGG GGCTCCTGAG CTTTGCAGAG TCAGCAAACT TGAATCTTAG CATTCATTTT 
GATGCTCCAG GCAGGCCCGC CATCTTCACC ATCAAGGACT CTTTGCTGGA CGGCCACTTT 
GTCTTGGCCA CACTCTCAGA CACCGACTCG CACTCCCAGG ACCTGGGCTC CCCAGAGCGT 
CACCAGCCAG TGCCTCAGCT CCAGGCTCAC AGCACACCCC ACCCGGACGA CTTTGCCAAT 
GACGACATTG ACTCTTACAT GATCGCCATG GAAACCACTA TAGGCAATGA GGGCTCGCGG 
GTGCTGCCCT CCATTTCCCT TTCACCTGGC CCCCAGCCCC CCAAGAGCCC CGGTCCCCAC 
TCCGAGGAGG AAGATGAGGC TGAGCCCAGT ACAGTGCCTG GGACTCCCCC ACCCAAGAAG 
TTCCGCTCAC TGTTCTTCGG CTCCATCCTG GCCCCTGTAC GCTCCCCCCA GGGCCCCAGC 
CCTGTGCTGG CGGAAGACAG TGAGGGTGAA GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004575.1
CDS	94..1269
Translation	MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

Target ORF information:

RefSeq Version	NM_004584.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004584.2

ORF Insert Sequence:

ATGAAGTGCC TGGTCACGGG CGGCAACGTG AAGGTGCTCG GCAAGGCCGT CCACTCCCTG 
TCCCGCATCG GGGACGAGCT CTACCTGGAA CCCTTGGAGG ACGGGCTCTC CCTCCGGACG 
GTGAACTCCT CCCGCTCTGC CTATGCCTGC TTTCTCTTTG CCCCGCTCTT CTTCCAGCAA 
TACCAGGCAG CCACCCCTGG TCAGGACCTG CTGCGCTGTA AGATCCTGAT GAAGTCTTTC 
CTGTCTGTCT TCCGCTCACT GGCGATGCTG GAGAAGACGG TGGAAAAATG CTGCATCTCC 
CTGAATGGCC GGAGCAGCCG CCTGGTGGTC CAGCTGCATT GCAAGTTCGG GGTGCGGAAG 
ACTCACAACC TGTCCTTCCA GGACTGTGAG TCCCTGCAGG CCGTCTTCGA CCCAGCCTCG 
TGCCCCCACA TGCTCCGCGC CCCAGCACGG GTTCTGGGGG AGGCTGTTCT GCCCTTCTCT 
CCTGCACTGG CTGAAGTGAC GCTGGGCATT GGCCGTGGCC GCAGGGTCAT CCTGCGCAGC 
TACCACGAGG AGGAGGCAGA CAGCACTGCC AAAGCCATGG TGACTGAGAT GTGCCTTGGA 
GAGGAGGATT TCCAGCAGCT GCAGGCCCAG GAAGGGGTGG CCATCACTTT CTGCCTCAAG 
GAATTCCGGG GGCTCCTGAG CTTTGCAGAG TCAGCAAACT TGAATCTTAG CATTCATTTT 
GATGCTCCAG GCAGGCCCGC CATCTTCACC ATCAAGGACT CTTTGCTGGA CGGCCACTTT 
GTCTTGGCCA CACTCTCAGA CACCGACTCG CACTCCCAGG ACCTGGGCTC CCCAGAGCGT 
CACCAGCCAG TGCCTCAGCT CCAGGCTCAC AGCACACCCC ACCCGGACGA CTTTGCCAAT 
GACGACATTG ACTCTTACAT GATCGCCATG GAAACCACTA TAGGCAATGA GGGCTCGCGG 
GTGCTGCCCT CCATTTCCCT TTCACCTGGC CCCCAGCCCC CCAAGAGCCC CGGTCCCCAC 
TCCGAGGAGG AAGATGAGGC TGAGCCCAGT ACAGTGCCTG GGACTCCCCC ACCCAAGAAG 
TTCCGCTCAC TGTTCTTCGG CTCCATCCTG GCCCCTGTAC GCTCCCCCCA GGGCCCCAGC 
CCTGTGCTGG CGGAAGACAG TGAGGGTGAA GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD9A ( NM_004584.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004575.1 Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04471
Clone ID Related Accession (Same CDS sequence)	NM_004584.2 , NM_004584.3
Accession Version	NM_004584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	cell cycle checkpoint control protein RAD9A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014848.1, U53174.1 and AA708970.1. On Nov 23, 2018 this sequence version replaced NM_004584.2. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53174.1, BC014848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000307980.7/ ENSP00000311360.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGTGCC TGGTCACGGG CGGCAACGTG AAGGTGCTCG GCAAGGCCGT CCACTCCCTG 
TCCCGCATCG GGGACGAGCT CTACCTGGAA CCCTTGGAGG ACGGGCTCTC CCTCCGGACG 
GTGAACTCCT CCCGCTCTGC CTATGCCTGC TTTCTCTTTG CCCCGCTCTT CTTCCAGCAA 
TACCAGGCAG CCACCCCTGG TCAGGACCTG CTGCGCTGTA AGATCCTGAT GAAGTCTTTC 
CTGTCTGTCT TCCGCTCACT GGCGATGCTG GAGAAGACGG TGGAAAAATG CTGCATCTCC 
CTGAATGGCC GGAGCAGCCG CCTGGTGGTC CAGCTGCATT GCAAGTTCGG GGTGCGGAAG 
ACTCACAACC TGTCCTTCCA GGACTGTGAG TCCCTGCAGG CCGTCTTCGA CCCAGCCTCG 
TGCCCCCACA TGCTCCGCGC CCCAGCACGG GTTCTGGGGG AGGCTGTTCT GCCCTTCTCT 
CCTGCACTGG CTGAAGTGAC GCTGGGCATT GGCCGTGGCC GCAGGGTCAT CCTGCGCAGC 
TACCACGAGG AGGAGGCAGA CAGCACTGCC AAAGCCATGG TGACTGAGAT GTGCCTTGGA 
GAGGAGGATT TCCAGCAGCT GCAGGCCCAG GAAGGGGTGG CCATCACTTT CTGCCTCAAG 
GAATTCCGGG GGCTCCTGAG CTTTGCAGAG TCAGCAAACT TGAATCTTAG CATTCATTTT 
GATGCTCCAG GCAGGCCCGC CATCTTCACC ATCAAGGACT CTTTGCTGGA CGGCCACTTT 
GTCTTGGCCA CACTCTCAGA CACCGACTCG CACTCCCAGG ACCTGGGCTC CCCAGAGCGT 
CACCAGCCAG TGCCTCAGCT CCAGGCTCAC AGCACACCCC ACCCGGACGA CTTTGCCAAT 
GACGACATTG ACTCTTACAT GATCGCCATG GAAACCACTA TAGGCAATGA GGGCTCGCGG 
GTGCTGCCCT CCATTTCCCT TTCACCTGGC CCCCAGCCCC CCAAGAGCCC CGGTCCCCAC 
TCCGAGGAGG AAGATGAGGC TGAGCCCAGT ACAGTGCCTG GGACTCCCCC ACCCAAGAAG 
TTCCGCTCAC TGTTCTTCGG CTCCATCCTG GCCCCTGTAC GCTCCCCCCA GGGCCCCAGC 
CCTGTGCTGG CGGAAGACAG TGAGGGTGAA GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004575.1
CDS	60..1235
Translation	MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

Target ORF information:

RefSeq Version	NM_004584.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004584.3

ORF Insert Sequence:

ATGAAGTGCC TGGTCACGGG CGGCAACGTG AAGGTGCTCG GCAAGGCCGT CCACTCCCTG 
TCCCGCATCG GGGACGAGCT CTACCTGGAA CCCTTGGAGG ACGGGCTCTC CCTCCGGACG 
GTGAACTCCT CCCGCTCTGC CTATGCCTGC TTTCTCTTTG CCCCGCTCTT CTTCCAGCAA 
TACCAGGCAG CCACCCCTGG TCAGGACCTG CTGCGCTGTA AGATCCTGAT GAAGTCTTTC 
CTGTCTGTCT TCCGCTCACT GGCGATGCTG GAGAAGACGG TGGAAAAATG CTGCATCTCC 
CTGAATGGCC GGAGCAGCCG CCTGGTGGTC CAGCTGCATT GCAAGTTCGG GGTGCGGAAG 
ACTCACAACC TGTCCTTCCA GGACTGTGAG TCCCTGCAGG CCGTCTTCGA CCCAGCCTCG 
TGCCCCCACA TGCTCCGCGC CCCAGCACGG GTTCTGGGGG AGGCTGTTCT GCCCTTCTCT 
CCTGCACTGG CTGAAGTGAC GCTGGGCATT GGCCGTGGCC GCAGGGTCAT CCTGCGCAGC 
TACCACGAGG AGGAGGCAGA CAGCACTGCC AAAGCCATGG TGACTGAGAT GTGCCTTGGA 
GAGGAGGATT TCCAGCAGCT GCAGGCCCAG GAAGGGGTGG CCATCACTTT CTGCCTCAAG 
GAATTCCGGG GGCTCCTGAG CTTTGCAGAG TCAGCAAACT TGAATCTTAG CATTCATTTT 
GATGCTCCAG GCAGGCCCGC CATCTTCACC ATCAAGGACT CTTTGCTGGA CGGCCACTTT 
GTCTTGGCCA CACTCTCAGA CACCGACTCG CACTCCCAGG ACCTGGGCTC CCCAGAGCGT 
CACCAGCCAG TGCCTCAGCT CCAGGCTCAC AGCACACCCC ACCCGGACGA CTTTGCCAAT 
GACGACATTG ACTCTTACAT GATCGCCATG GAAACCACTA TAGGCAATGA GGGCTCGCGG 
GTGCTGCCCT CCATTTCCCT TTCACCTGGC CCCCAGCCCC CCAAGAGCCC CGGTCCCCAC 
TCCGAGGAGG AAGATGAGGC TGAGCCCAGT ACAGTGCCTG GGACTCCCCC ACCCAAGAAG 
TTCCGCTCAC TGTTCTTCGG CTCCATCCTG GCCCCTGTAC GCTCCCCCCA GGGCCCCAGC 
CCTGTGCTGG CGGAAGACAG TGAGGGTGAA GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD9A ( XM_017018098.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873587.1 Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X4, mRNA.

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CloneID	OHu60166
Clone ID Related Accession (Same CDS sequence)	XM_017018098.1
Accession Version	XM_017018098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell cycle checkpoint control protein RAD9A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACTG AGATGTGCCT TGGAGAGGAG GATTTCCAGC AGCTGCAGGC CCAGGAAGGG 
GTGGCCATCA CTTTCTGCCT CAAGGAATTC CGGGGGCTCC TGAGCTTTGC AGAGTCAGCA 
AACTTGAATC TTAGCATTCA TTTTGATGCT CCAGGCAGGC CCGCCATCTT CACCATCAAG 
GACTCTTTGC TGGACGGCCA CTTTGTCTTG GCCACACTCT CAGACACCGA CTCGCACTCC 
CAGGACCTGG GCTCCCCAGA GCGTCACCAG CCAGTGCCTC AGCTCCAGGC TCACAGCACA 
CCCCACCCGG ACGACTTTGC CAATGACGAC ATTGACTCTT ACATGATCGC CATGGAAACC 
ACTATAGGCA ATGAGGGCTC GCGGGTGCTG CCCTCCATTT CCCTTTCACC TGGCCCCCAG 
CCCCCCAAGA GCCCCGGTCC CCACTCCGAG GAGGAAGATG AGGCTGAGCC CAGTACAGTG 
CCTGGGACTC CCCCACCCAA GAAGTTCCGC TCACTGTTCT TCGGCTCCAT CCTGGCCCCT 
GTACGCTCCC CCCAGGGCCC CAGCCCTGTG CTGGCGGAAG ACAGTGAGGG TGAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873587.1
CDS	406..1005
Translation	MVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

Target ORF information:

RefSeq Version	XM_017018098.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018098.1

ORF Insert Sequence:

ATGGTGACTG AGATGTGCCT TGGAGAGGAG GATTTCCAGC AGCTGCAGGC CCAGGAAGGG 
GTGGCCATCA CTTTCTGCCT CAAGGAATTC CGGGGGCTCC TGAGCTTTGC AGAGTCAGCA 
AACTTGAATC TTAGCATTCA TTTTGATGCT CCAGGCAGGC CCGCCATCTT CACCATCAAG 
GACTCTTTGC TGGACGGCCA CTTTGTCTTG GCCACACTCT CAGACACCGA CTCGCACTCC 
CAGGACCTGG GCTCCCCAGA GCGTCACCAG CCAGTGCCTC AGCTCCAGGC TCACAGCACA 
CCCCACCCGG ACGACTTTGC CAATGACGAC ATTGACTCTT ACATGATCGC CATGGAAACC 
ACTATAGGCA ATGAGGGCTC GCGGGTGCTG CCCTCCATTT CCCTTTCACC TGGCCCCCAG 
CCCCCCAAGA GCCCCGGTCC CCACTCCGAG GAGGAAGATG AGGCTGAGCC CAGTACAGTG 
CCTGGGACTC CCCCACCCAA GAAGTTCCGC TCACTGTTCT TCGGCTCCAT CCTGGCCCCT 
GTACGCTCCC CCCAGGGCCC CAGCCCTGTG CTGGCGGAAG ACAGTGAGGG TGAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD9A ( XM_006718652.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718715.1 Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu45452
Clone ID Related Accession (Same CDS sequence)	XM_006718652.3
Accession Version	XM_006718652.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell cycle checkpoint control protein RAD9A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAG GAGGGTTTTT CAGCAGGTGG TGGCGGAGCG GGAGGACGAT AGGGCAAGTG 
TGTGAGCAGA AGCAGCCAGA GGGCTGGGTC TGTGGCTGCC CCCTGACCAC GTCCCTCTCC 
CTGCTCTTCG TGGCCCAGCT CTCCCTCCGG ACGGTGAACT CCTCCCGCTC TGCCTATGCC 
TGCTTTCTCT TTGCCCCGCT CTTCTTCCAG CAATACCAGG CAGCCACCCC TGGTCAGGAC 
CTGCTGCGCT GTAAGATCCT GATGAAGTCT TTCCTGTCTG TCTTCCGCTC ACTGGCGATG 
CTGGAGAAGA CGGTGGAAAA ATGCTGCATC TCCCTGAATG GCCGGAGCAG CCGCCTGGTG 
GTCCAGCTGC ATTGCAAGTT CGGGGTGCGG AAGACTCACA ACCTGTCCTT CCAGGACTGT 
GAGTCCCTGC AGGCCGTCTT CGACCCAGCC TCGTGCCCCC ACATGCTCCG CGCCCCAGCA 
CGGGTTCTGG GGGAGGCTGT TCTGCCCTTC TCTCCTGCAC TGGCTGAAGT GACGCTGGGC 
ATTGGCCGTG GCCGCAGGGT CATCCTGCGC AGCTACCACG AGGAGGAGGC AGACAGCACT 
GCCAAAGCCA TGGTGACTGA GATGTGCCTT GGAGAGGAGG ATTTCCAGCA GCTGCAGGCC 
CAGGAAGGGG TGGCCATCAC TTTCTGCCTC AAGGAATTCC GGGGGCTCCT GAGCTTTGCA 
GAGTCAGCAA ACTTGAATCT TAGCATTCAT TTTGATGCTC CAGGCAGGCC CGCCATCTTC 
ACCATCAAGG ACTCTTTGCT GGACGGCCAC TTTGTCTTGG CCACACTCTC AGACACCGAC 
TCGCACTCCC AGGACCTGGG CTCCCCAGAG CGTCACCAGC CAGTGCCTCA GCTCCAGGCT 
CACAGCACAC CCCACCCGGA CGACTTTGCC AATGACGACA TTGACTCTTA CATGATCGCC 
ATGGAAACCA CTATAGGCAA TGAGGGCTCG CGGGTGCTGC CCTCCATTTC CCTTTCACCT 
GGCCCCCAGC CCCCCAAGAG CCCCGGTCCC CACTCCGAGG AGGAAGATGA GGCTGAGCCC 
AGTACAGTGC CTGGGACTCC CCCACCCAAG AAGTTCCGCT CACTGTTCTT CGGCTCCATC 
CTGGCCCCTG TACGCTCCCC CCAGGGCCCC AGCCCTGTGC TGGCGGAAGA CAGTGAGGGT 
GAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718715.1
CDS	273..1481
Translation	MGKGGFFSRWWRSGRTIGQVCEQKQPEGWVCGCPLTTSLSLLFVAQLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

Target ORF information:

RefSeq Version	XM_006718652.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718652.3

ORF Insert Sequence:

ATGGGGAAAG GAGGGTTTTT CAGCAGGTGG TGGCGGAGCG GGAGGACGAT AGGGCAAGTG 
TGTGAGCAGA AGCAGCCAGA GGGCTGGGTC TGTGGCTGCC CCCTGACCAC GTCCCTCTCC 
CTGCTCTTCG TGGCCCAGCT CTCCCTCCGG ACGGTGAACT CCTCCCGCTC TGCCTATGCC 
TGCTTTCTCT TTGCCCCGCT CTTCTTCCAG CAATACCAGG CAGCCACCCC TGGTCAGGAC 
CTGCTGCGCT GTAAGATCCT GATGAAGTCT TTCCTGTCTG TCTTCCGCTC ACTGGCGATG 
CTGGAGAAGA CGGTGGAAAA ATGCTGCATC TCCCTGAATG GCCGGAGCAG CCGCCTGGTG 
GTCCAGCTGC ATTGCAAGTT CGGGGTGCGG AAGACTCACA ACCTGTCCTT CCAGGACTGT 
GAGTCCCTGC AGGCCGTCTT CGACCCAGCC TCGTGCCCCC ACATGCTCCG CGCCCCAGCA 
CGGGTTCTGG GGGAGGCTGT TCTGCCCTTC TCTCCTGCAC TGGCTGAAGT GACGCTGGGC 
ATTGGCCGTG GCCGCAGGGT CATCCTGCGC AGCTACCACG AGGAGGAGGC AGACAGCACT 
GCCAAAGCCA TGGTGACTGA GATGTGCCTT GGAGAGGAGG ATTTCCAGCA GCTGCAGGCC 
CAGGAAGGGG TGGCCATCAC TTTCTGCCTC AAGGAATTCC GGGGGCTCCT GAGCTTTGCA 
GAGTCAGCAA ACTTGAATCT TAGCATTCAT TTTGATGCTC CAGGCAGGCC CGCCATCTTC 
ACCATCAAGG ACTCTTTGCT GGACGGCCAC TTTGTCTTGG CCACACTCTC AGACACCGAC 
TCGCACTCCC AGGACCTGGG CTCCCCAGAG CGTCACCAGC CAGTGCCTCA GCTCCAGGCT 
CACAGCACAC CCCACCCGGA CGACTTTGCC AATGACGACA TTGACTCTTA CATGATCGCC 
ATGGAAACCA CTATAGGCAA TGAGGGCTCG CGGGTGCTGC CCTCCATTTC CCTTTCACCT 
GGCCCCCAGC CCCCCAAGAG CCCCGGTCCC CACTCCGAGG AGGAAGATGA GGCTGAGCCC 
AGTACAGTGC CTGGGACTCC CCCACCCAAG AAGTTCCGCT CACTGTTCTT CGGCTCCATC 
CTGGCCCCTG TACGCTCCCC CCAGGGCCCC AGCCCTGTGC TGGCGGAAGA CAGTGAGGGT 
GAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD9A ( XM_017018097.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873586.1 Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu92413
Clone ID Related Accession (Same CDS sequence)	XM_017018097.1
Accession Version	XM_017018097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell cycle checkpoint control protein RAD9A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG CAGCCGCCTG GTGGTCCAGC TGCATTGCAA GTTCGGACTG TGAGTCCCTG 
CAGGCCGTCT TCGACCCAGC CTCGTGCCCC CACATGCTCC GCGCCCCAGC ACGGGTTCTG 
GGGGAGGCTG TTCTGCCCTT CTCTCCTGCA CTGGCTGAAG TGACGCTGGG CATTGGCCGT 
GGCCGCAGGG TCATCCTGCG CAGCTACCAC GAGGAGGAGG CAGACAGCAC TGCCAAAGCC 
ATGGTGACTG AGATGTGCCT TGGAGAGGAG GATTTCCAGC AGCTGCAGGC CCAGGAAGGG 
GTGGCCATCA CTTTCTGCCT CAAGGAATTC CGGGGGCTCC TGAGCTTTGC AGAGTCAGCA 
AACTTGAATC TTAGCATTCA TTTTGATGCT CCAGGCAGGC CCGCCATCTT CACCATCAAG 
GACTCTTTGC TGGACGGCCA CTTTGTCTTG GCCACACTCT CAGACACCGA CTCGCACTCC 
CAGGACCTGG GCTCCCCAGA GCGTCACCAG CCAGTGCCTC AGCTCCAGGC TCACAGCACA 
CCCCACCCGG ACGACTTTGC CAATGACGAC ATTGACTCTT ACATGATCGC CATGGAAACC 
ACTATAGGCA ATGAGGGCTC GCGGGTGCTG CCCTCCATTT CCCTTTCACC TGGCCCCCAG 
CCCCCCAAGA GCCCCGGTCC CCACTCCGAG GAGGAAGATG AGGCTGAGCC CAGTACAGTG 
CCTGGGACTC CCCCACCCAA GAAGTTCCGC TCACTGTTCT TCGGCTCCAT CCTGGCCCCT 
GTACGCTCCC CCCAGGGCCC CAGCCCTGTG CTGGCGGAAG ACAGTGAGGG TGAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873586.1
CDS	374..1213
Translation	MAGAAAWWSSCIASSDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

Target ORF information:

RefSeq Version	XM_017018097.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018097.1

ORF Insert Sequence:

ATGGCCGGAG CAGCCGCCTG GTGGTCCAGC TGCATTGCAA GTTCGGACTG TGAGTCCCTG 
CAGGCCGTCT TCGACCCAGC CTCGTGCCCC CACATGCTCC GCGCCCCAGC ACGGGTTCTG 
GGGGAGGCTG TTCTGCCCTT CTCTCCTGCA CTGGCTGAAG TGACGCTGGG CATTGGCCGT 
GGCCGCAGGG TCATCCTGCG CAGCTACCAC GAGGAGGAGG CAGACAGCAC TGCCAAAGCC 
ATGGTGACTG AGATGTGCCT TGGAGAGGAG GATTTCCAGC AGCTGCAGGC CCAGGAAGGG 
GTGGCCATCA CTTTCTGCCT CAAGGAATTC CGGGGGCTCC TGAGCTTTGC AGAGTCAGCA 
AACTTGAATC TTAGCATTCA TTTTGATGCT CCAGGCAGGC CCGCCATCTT CACCATCAAG 
GACTCTTTGC TGGACGGCCA CTTTGTCTTG GCCACACTCT CAGACACCGA CTCGCACTCC 
CAGGACCTGG GCTCCCCAGA GCGTCACCAG CCAGTGCCTC AGCTCCAGGC TCACAGCACA 
CCCCACCCGG ACGACTTTGC CAATGACGAC ATTGACTCTT ACATGATCGC CATGGAAACC 
ACTATAGGCA ATGAGGGCTC GCGGGTGCTG CCCTCCATTT CCCTTTCACC TGGCCCCCAG 
CCCCCCAAGA GCCCCGGTCC CCACTCCGAG GAGGAAGATG AGGCTGAGCC CAGTACAGTG 
CCTGGGACTC CCCCACCCAA GAAGTTCCGC TCACTGTTCT TCGGCTCCAT CCTGGCCCCT 
GTACGCTCCC CCCAGGGCCC CAGCCCTGTG CTGGCGGAAG ACAGTGAGGG TGAAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The CDK-PLK1 axis targets the DNA damage checkpoint sensor protein RAD9 to promote cell proliferation and tolerance to genotoxic stress.
eLife6(2017 Dec)
Wakida T,Ikura M,Kuriya K,Ito S,Shiroiwa Y,Habu T,Kawamoto T,Okumura K,Ikura T,Furuya K

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint.
BMC molecular biology173(2016 Feb)
Awate S,De Benedetti A

Regulation of ATRIP protein abundance by RAD9 in the DNA damage repair pathway.
Cellular and molecular biology (Noisy-le-Grand, France)61(8)31-6(2015 Dec)
Peng XJ,Liu SJ,Bao CM,Liu YZ,Xie HW,Cai YH,Li BM,Hang HY,Ding X

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RAD9A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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