BCAT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCAT2
Entrez Gene ID	587
Full Name	branched chain amino acid transaminase 2
Synonyms	BCAM,BCATM,BCT2,PP18
General protein information	Preferred Names branched chain amino acid transaminase 2 Names branched-chain-amino-acid aminotransferase, mitochondrial branched chain amino-acid transaminase 2, mitochondrial branched chain aminotransferase 2, mitochondrial placental protein 18
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].
Disorder MIM:	113530
Disorder Html:	?Hypervalinemia or hyperleucine-isoleucinemia (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001284325.2	NP_001271254.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform c
NM_001164773.1	NP_001158245.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform b
NM_001284325.1	NP_001271254.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform c
NM_001190.3	NP_001181.2	branched-chain-amino-acid aminotransferase, mitochondrial isoform a precursor
NM_001164773.2	NP_001158245.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform b
XM_024451630.1	XP_024307398.1	branched-chain-amino-acid aminotransferase, mitochondrial isoform X1
NM_001190.4	NP_001181.2	branched-chain-amino-acid aminotransferase, mitochondrial isoform a precursor

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A10307g	XP_451451.1
Schizosaccharomyces pombe (fission yeast)	eca39	NP_595180.2
Magnaporthe oryzae (rice blast fungus)	MGG_02489	XP_003709286.1
Neurospora crassa	NCU04754	XP_960287.1
Pan troglodytes (chimpanzee)	BCAT2	XP_001171838.2
Canis lupus familiaris (dog)	BCAT2	XP_541509.3
Bos taurus (cattle)	BCAT2	NP_001013611.2
Mus musculus (house mouse)	Bcat2	NP_033867.1
Rattus norvegicus (Norway rat)	Bcat2	NP_071795.1
Saccharomyces cerevisiae (baker's yeast)	BAT1	NP_012078.3
Oryza sativa (rice)	Os03g0231600	NP_001049461.1
Xenopus tropicalis (tropical clawed frog)	bcat2	NP_001006740.1
Homo sapiens (human)	BCAT2	NP_001181.2
	BCAT2	XP_001114272.1
Danio rerio (zebrafish)	bcat2	NP_001002676.1
Arabidopsis thaliana (thale cress)	BCAT-2	NP_001031015.1

Gene Ontology
Bibliography

Related articles in PubMed

Branched-chain amino acid metabolism in cancer.
Ananieva EA, Wilkinson AC
Current opinion in clinical nutrition and metabolic care21(1)64-70(2018 Jan)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation.
Hull J, Hindy ME, Kehoe PG, Chalmers K, Love S, Conway ME
Journal of neurochemistry123(6)997-1009(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mitochondrial isoform human brain BCAT 2 is largely confined to vascular endothelial cells, whereas the cytosolic human brain BCAT 1 is restricted to neurons.
Title: Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation.

BCATc (cytosolic) has an overall redox potential that is 30 mV lower than BCATm (mitochondrial). Furthermore, the CXXC motif of BCATc was estimated to be 80 mV lower, suggesting that BCATm is more oxidizing in nature.
Title: Differential redox potential between the human cytosolic and mitochondrial branched-chain aminotransferase.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The effect of nitric oxide modification on the functionality of human mitochondrial and cytosolic branched-chain aminotransferases (hBCATm and hBCATc, respectively) was investigated.
Title: S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation.

ption of the CXXC center results in altered substrate orientation and deprotonation of the amino group of pyridoxamine 5'-phosphate, which inhibits catalysis
Title: Human mitochondrial branched chain aminotransferase isozyme: structural role of the CXXC center in catalysis.

The following BCAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10011	NM_001284325.2 Latest version!	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu18831	NM_001190.4 Latest version!	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10011	XM_024451630.1 Latest version!	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu09494	NM_001164773.2 Latest version!	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu10011	NM_001284325.1	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu09494	NM_001164773.1	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu18831	NM_001190.3	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCAT2 ( NM_001284325.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271254.1 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant c, mRNA.

Price & Availability↑

CloneID	OHu10011
Clone ID Related Accession (Same CDS sequence)	NM_001284325.2 , NM_001284325.1 , XM_024451630.1
Accession Version	NM_001284325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM802402.1, AK093627.1, BC001900.1 and CA310873.1. On May 31, 2019 this sequence version replaced NM_001284325.1. Transcript Variant: This variant (c) uses an alternate 5' exon structure and thus differs in the 5' UTR and 5' coding region, compared to variant a. These differences cause translation initiation at a downstream start codon and result in an isoform (c) with a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.656777.1, SRR1803611.156602.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271254.1
CDS	624..1682
Translation	MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	NM_001284325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284325.2

ORF Insert Sequence:

ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAT2 ( NM_001284325.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271254.1 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant c, mRNA.

Price & Availability↑

CloneID	OHu10011
Clone ID Related Accession (Same CDS sequence)	NM_001284325.2 , NM_001284325.1 , XM_024451630.1
Accession Version	NM_001284325.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129578.1, BM802402.1, AK093627.1, BC001900.1 and CA310873.1. On Sep 28, 2013 this sequence version replaced NR_028450.1. Transcript Variant: This variant (c) uses an alternate 5' exon structure and thus differs in the 5' UTR and 5' coding region, compared to variant a. These differences cause translation initiation at a downstream start codon and result in an isoform (c) with a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093627.1, SRR1803611.156602.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271254.1
CDS	658..1716
Translation	MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	NM_001284325.1
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284325.1

ORF Insert Sequence:

ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAT2 ( NM_001190.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001181.2 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant a, mRNA.

Price & Availability↑

CloneID	OHu18831
Clone ID Related Accession (Same CDS sequence)	NM_001190.3 , NM_001190.4
Accession Version	NM_001190.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129578.1, BC001900.1 and CA310873.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2009 this sequence version replaced NM_001190.2. Transcript Variant: This variant (a) encodes the longest isoform (a), also known as PP18a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.311264.1, AK223522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAG CCGCTCTGGG GCAGATCTGG GCACGAAAGC TTCTCTCTGT CCCTTGGCTT 
CTGTGTGGTC CCAGAAGATA TGCCTCCTCC AGTTTCAAGG CTGCAGACCT GCAGCTGGAA 
ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181.2
CDS	57..1235
Translation	MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	NM_001190.3
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190.3

ORF Insert Sequence:

ATGGCCGCAG CCGCTCTGGG GCAGATCTGG GCACGAAAGC TTCTCTCTGT CCCTTGGCTT 
CTGTGTGGTC CCAGAAGATA TGCCTCCTCC AGTTTCAAGG CTGCAGACCT GCAGCTGGAA 
ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAT2 ( NM_001190.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001181.2 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant a, mRNA.

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CloneID	OHu18831
Clone ID Related Accession (Same CDS sequence)	NM_001190.3 , NM_001190.4
Accession Version	NM_001190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129578.1, BC001900.1 and CA310873.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001190.3. Transcript Variant: This variant (a) encodes the longest isoform (a), also known as PP18a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.87615.1, AK223522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000316273.11/ ENSP00000322991.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCAG CCGCTCTGGG GCAGATCTGG GCACGAAAGC TTCTCTCTGT CCCTTGGCTT 
CTGTGTGGTC CCAGAAGATA TGCCTCCTCC AGTTTCAAGG CTGCAGACCT GCAGCTGGAA 
ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001181.2
CDS	23..1201
Translation	MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	NM_001190.4
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190.4

ORF Insert Sequence:

ATGGCCGCAG CCGCTCTGGG GCAGATCTGG GCACGAAAGC TTCTCTCTGT CCCTTGGCTT 
CTGTGTGGTC CCAGAAGATA TGCCTCCTCC AGTTTCAAGG CTGCAGACCT GCAGCTGGAA 
ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAT2 ( XM_024451630.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307398.1 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant X1, mRNA.

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CloneID	OHu10011
Clone ID Related Accession (Same CDS sequence)	NM_001284325.2 , NM_001284325.1 , XM_024451630.1
Accession Version	XM_024451630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307398.1
CDS	247..1305
Translation	MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	XM_024451630.1
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451630.1

ORF Insert Sequence:

ATGACACAGA AGCCTCATAA GAAGCCTGGC CCCGGCGAGC CCCTGGTGTT TGGGAAGACA 
TTTACCGACC ACATGCTGAT GGTGGAATGG AATGACAAGG GCTGGGGCCA GCCCCGAATC 
CAGCCCTTCC AGAACCTCAC GCTGCACCCA GCCTCCTCCA GCCTCCACTA CTCCCTGCAG 
CTGTTTGAGG GCATGAAGGC GTTCAAAGGC AAAGACCAGC AGGTGCGCCT CTTCCGCCCC 
TGGCTCAACA TGGACCGGAT GCTGCGCTCA GCCATGCGCC TGTGCCTGCC GAGTTTCGAC 
AAGCTGGAGT TGCTGGAGTG CATCCGCCGG CTCATCGAAG TGGACAAGGA CTGGGTCCCC 
GATGCCGCCG GCACCAGCCT CTATGTGCGG CCTGTGCTCA TTGGGAACGA GCCCTCGCTG 
GGTGTCAGCC AGCCCACGCG CGCGCTCCTG TTCGTCATTC TCTGCCCAGT GGGTGCCTAC 
TTCCCTGGAG GCTCCGTGAC CCCGGTCTCC CTCCTGGCCG ACCCAGCCTT CATCCGGGCC 
TGGGTGGGCG GGGTCGGCAA CTACAAGTTA GGTGGGAATT ATGGGCCCAC CGTGTTAGTG 
CAACAGGAGG CACTCAAGCG GGGCTGTGAA CAGGTCCTCT GGCTGTATGG GCCCGACCAC 
CAGCTCACCG AGGTGGGAAC CATGAACATC TTTGTCTACT GGACCCACGA AGATGGGGTG 
CTGGAGCTGG TGACGCCCCC GCTGAATGGT GTTATCCTGC CTGGAGTGGT CAGACAGAGT 
CTACTGGACA TGGCTCAGAC CTGGGGTGAG TTCCGGGTGG TGGAGCGCAC GATCACCATG 
AAGCAGTTGC TGCGGGCCCT GGAGGAGGGC CGCGTGCGGG AAGTCTTTGG CTCGGGCACC 
GCTTGCCAGG TCTGCCCAGT GCACCGAATC CTGTACAAAG ACAGGAACCT CCACATTCCC 
ACCATGGAAA ATGGGCCTGA GCTGATCCTC CGCTTCCAGA AGGAGCTGAA GGAGATCCAG 
TACGGAATCA GAGCCCACGA GTGGATGTTC CCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAT2 ( NM_001164773.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158245.1 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant b, mRNA.

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CloneID	OHu09494
Clone ID Related Accession (Same CDS sequence)	NM_001164773.1 , NM_001164773.2
Accession Version	NM_001164773.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129578.1, AF268048.1 and CA310873.1. Transcript Variant: This variant (b) lacks two alternate in-frame exons in the 5' coding region, compared to variant a. The resulting isoform (b), also known as PP18b, lacks an internal segment near the N-terminus, compared to isoform a, which disrupts the transit peptide. This isoform is found in the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF268048.1, SRR1163658.76314.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAG CCGCTCTGGG GCAGCTGTTT GAGGGCATGA AGGCGTTCAA AGGCAAAGAC 
CAGCAGGTGC GCCTCTTCCG CCCCTGGCTC AACATGGACC GGATGCTGCG CTCAGCCATG 
CGCCTGTGCC TGCCGAGTTT CGACAAGCTG GAGTTGCTGG AGTGCATCCG CCGGCTCATC 
GAAGTGGACA AGGACTGGGT CCCCGATGCC GCCGGCACCA GCCTCTATGT GCGGCCTGTG 
CTCATTGGGA ACGAGCCCTC GCTGGGTGTC AGCCAGCCCA CGCGCGCGCT CCTGTTCGTC 
ATTCTCTGCC CAGTGGGTGC CTACTTCCCT GGAGGCTCCG TGACCCCGGT CTCCCTCCTG 
GCCGACCCAG CCTTCATCCG GGCCTGGGTG GGCGGGGTCG GCAACTACAA GTTAGGTGGG 
AATTATGGGC CCACCGTGTT AGTGCAACAG GAGGCACTCA AGCGGGGCTG TGAACAGGTC 
CTCTGGCTGT ATGGGCCCGA CCACCAGCTC ACCGAGGTGG GAACCATGAA CATCTTTGTC 
TACTGGACCC ACGAAGATGG GGTGCTGGAG CTGGTGACGC CCCCGCTGAA TGGTGTTATC 
CTGCCTGGAG TGGTCAGACA GAGTCTACTG GACATGGCTC AGACCTGGGG TGAGTTCCGG 
GTGGTGGAGC GCACGATCAC CATGAAGCAG TTGCTGCGGG CCCTGGAGGA GGGCCGCGTG 
CGGGAAGTCT TTGGCTCGGG CACCGCTTGC CAGGTCTGCC CAGTGCACCG AATCCTGTAC 
AAAGACAGGA ACCTCCACAT TCCCACCATG GAAAATGGGC CTGAGCTGAT CCTCCGCTTC 
CAGAAGGAGC TGAAGGAGAT CCAGTACGGA ATCAGAGCCC ACGAGTGGAT GTTCCCGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158245.1
CDS	57..959
Translation	MAAAALGQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	NM_001164773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164773.1

ORF Insert Sequence:

ATGGCCGCAG CCGCTCTGGG GCAGCTGTTT GAGGGCATGA AGGCGTTCAA AGGCAAAGAC 
CAGCAGGTGC GCCTCTTCCG CCCCTGGCTC AACATGGACC GGATGCTGCG CTCAGCCATG 
CGCCTGTGCC TGCCGAGTTT CGACAAGCTG GAGTTGCTGG AGTGCATCCG CCGGCTCATC 
GAAGTGGACA AGGACTGGGT CCCCGATGCC GCCGGCACCA GCCTCTATGT GCGGCCTGTG 
CTCATTGGGA ACGAGCCCTC GCTGGGTGTC AGCCAGCCCA CGCGCGCGCT CCTGTTCGTC 
ATTCTCTGCC CAGTGGGTGC CTACTTCCCT GGAGGCTCCG TGACCCCGGT CTCCCTCCTG 
GCCGACCCAG CCTTCATCCG GGCCTGGGTG GGCGGGGTCG GCAACTACAA GTTAGGTGGG 
AATTATGGGC CCACCGTGTT AGTGCAACAG GAGGCACTCA AGCGGGGCTG TGAACAGGTC 
CTCTGGCTGT ATGGGCCCGA CCACCAGCTC ACCGAGGTGG GAACCATGAA CATCTTTGTC 
TACTGGACCC ACGAAGATGG GGTGCTGGAG CTGGTGACGC CCCCGCTGAA TGGTGTTATC 
CTGCCTGGAG TGGTCAGACA GAGTCTACTG GACATGGCTC AGACCTGGGG TGAGTTCCGG 
GTGGTGGAGC GCACGATCAC CATGAAGCAG TTGCTGCGGG CCCTGGAGGA GGGCCGCGTG 
CGGGAAGTCT TTGGCTCGGG CACCGCTTGC CAGGTCTGCC CAGTGCACCG AATCCTGTAC 
AAAGACAGGA ACCTCCACAT TCCCACCATG GAAAATGGGC CTGAGCTGAT CCTCCGCTTC 
CAGAAGGAGC TGAAGGAGAT CCAGTACGGA ATCAGAGCCC ACGAGTGGAT GTTCCCGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAT2 ( NM_001164773.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158245.1 Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant b, mRNA.

Price & Availability↑

CloneID	OHu09494
Clone ID Related Accession (Same CDS sequence)	NM_001164773.1 , NM_001164773.2
Accession Version	NM_001164773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	branched-chain-amino-acid aminotransferase, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA129578.1 and AF268048.1. On May 31, 2019 this sequence version replaced NM_001164773.1. Transcript Variant: This variant (b) lacks two alternate in-frame exons in the 5' coding region, compared to variant a. The resulting isoform (b), also known as PP18b, lacks an internal segment near the N-terminus, compared to isoform a, which disrupts the transit peptide. This isoform is found in the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF268048.1, SRR1163658.76314.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAG CCGCTCTGGG GCAGCTGTTT GAGGGCATGA AGGCGTTCAA AGGCAAAGAC 
CAGCAGGTGC GCCTCTTCCG CCCCTGGCTC AACATGGACC GGATGCTGCG CTCAGCCATG 
CGCCTGTGCC TGCCGAGTTT CGACAAGCTG GAGTTGCTGG AGTGCATCCG CCGGCTCATC 
GAAGTGGACA AGGACTGGGT CCCCGATGCC GCCGGCACCA GCCTCTATGT GCGGCCTGTG 
CTCATTGGGA ACGAGCCCTC GCTGGGTGTC AGCCAGCCCA CGCGCGCGCT CCTGTTCGTC 
ATTCTCTGCC CAGTGGGTGC CTACTTCCCT GGAGGCTCCG TGACCCCGGT CTCCCTCCTG 
GCCGACCCAG CCTTCATCCG GGCCTGGGTG GGCGGGGTCG GCAACTACAA GTTAGGTGGG 
AATTATGGGC CCACCGTGTT AGTGCAACAG GAGGCACTCA AGCGGGGCTG TGAACAGGTC 
CTCTGGCTGT ATGGGCCCGA CCACCAGCTC ACCGAGGTGG GAACCATGAA CATCTTTGTC 
TACTGGACCC ACGAAGATGG GGTGCTGGAG CTGGTGACGC CCCCGCTGAA TGGTGTTATC 
CTGCCTGGAG TGGTCAGACA GAGTCTACTG GACATGGCTC AGACCTGGGG TGAGTTCCGG 
GTGGTGGAGC GCACGATCAC CATGAAGCAG TTGCTGCGGG CCCTGGAGGA GGGCCGCGTG 
CGGGAAGTCT TTGGCTCGGG CACCGCTTGC CAGGTCTGCC CAGTGCACCG AATCCTGTAC 
AAAGACAGGA ACCTCCACAT TCCCACCATG GAAAATGGGC CTGAGCTGAT CCTCCGCTTC 
CAGAAGGAGC TGAAGGAGAT CCAGTACGGA ATCAGAGCCC ACGAGTGGAT GTTCCCGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158245.1
CDS	23..925
Translation	MAAAALGQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Target ORF information:

RefSeq Version	NM_001164773.2
Organism	Homo sapiens(human)
Definition	Homo sapiens branched chain amino acid transaminase 2 (BCAT2), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164773.2

ORF Insert Sequence:

ATGGCCGCAG CCGCTCTGGG GCAGCTGTTT GAGGGCATGA AGGCGTTCAA AGGCAAAGAC 
CAGCAGGTGC GCCTCTTCCG CCCCTGGCTC AACATGGACC GGATGCTGCG CTCAGCCATG 
CGCCTGTGCC TGCCGAGTTT CGACAAGCTG GAGTTGCTGG AGTGCATCCG CCGGCTCATC 
GAAGTGGACA AGGACTGGGT CCCCGATGCC GCCGGCACCA GCCTCTATGT GCGGCCTGTG 
CTCATTGGGA ACGAGCCCTC GCTGGGTGTC AGCCAGCCCA CGCGCGCGCT CCTGTTCGTC 
ATTCTCTGCC CAGTGGGTGC CTACTTCCCT GGAGGCTCCG TGACCCCGGT CTCCCTCCTG 
GCCGACCCAG CCTTCATCCG GGCCTGGGTG GGCGGGGTCG GCAACTACAA GTTAGGTGGG 
AATTATGGGC CCACCGTGTT AGTGCAACAG GAGGCACTCA AGCGGGGCTG TGAACAGGTC 
CTCTGGCTGT ATGGGCCCGA CCACCAGCTC ACCGAGGTGG GAACCATGAA CATCTTTGTC 
TACTGGACCC ACGAAGATGG GGTGCTGGAG CTGGTGACGC CCCCGCTGAA TGGTGTTATC 
CTGCCTGGAG TGGTCAGACA GAGTCTACTG GACATGGCTC AGACCTGGGG TGAGTTCCGG 
GTGGTGGAGC GCACGATCAC CATGAAGCAG TTGCTGCGGG CCCTGGAGGA GGGCCGCGTG 
CGGGAAGTCT TTGGCTCGGG CACCGCTTGC CAGGTCTGCC CAGTGCACCG AATCCTGTAC 
AAAGACAGGA ACCTCCACAT TCCCACCATG GAAAATGGGC CTGAGCTGAT CCTCCGCTTC 
CAGAAGGAGC TGAAGGAGAT CCAGTACGGA ATCAGAGCCC ACGAGTGGAT GTTCCCGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Branched-chain amino acid metabolism in cancer.
Current opinion in clinical nutrition and metabolic care21(1)64-70(2018 Jan)
Ananieva EA,Wilkinson AC

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation.
Journal of neurochemistry123(6)997-1009(2012 Dec)
Hull J,Hindy ME,Kehoe PG,Chalmers K,Love S,Conway ME

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BCAT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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