NLRC4 cDNA ORF clone, Homo sapiens(human)

The following NLRC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu70975 NM_001302504.1
Latest version!
Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu89747 XM_017004619.1
Latest version!
Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu16706 NM_021209.4
Latest version!
Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $384.30
$549.00
OHu16706 NM_001199138.2
Latest version!
Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $384.30
$549.00
OHu16706 NM_001199139.1
Latest version!
Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $384.30
$549.00
View Old Accession Versions >>
OHu16706 NM_001199138.1 Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu70975
    Clone ID Related Accession (Same CDS sequence) NM_001302504.1
    Accession Version NM_001302504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product NLR family CARD domain-containing protein 4 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121653.2, AY027788.1 and AW337918.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY027788.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTGGTCTG ACTGACAGCT TGGGTAACTT GAAGAACCTT
    ACAAAGCTCA TAATGGATAA CATAAAGATG AATGAAGAAG ATGCTATAAA ACTAGCTGAA
    GGCCTGAAAA ACCTGAAGAA GATGTGTTTA TTTCATTTGA CCCACTTGTC TGACATTGGA
    GAGGGAATGG ATTACATAGT CAAGTCTCTG TCAAGTGAAC CCTGTGACCT TGAAGAAATT
    CAATTAGTCT CCTGCTGCTT GTCTGCAAAT GCAGTGAAAA TCCTAGCTCA GAATCTTCAC
    AATTTGGTCA AACTGAGCAT TCTTGATTTA TCAGAAAATT ACCTGGAAAA AGATGGAAAT
    GAAGCTCTTC ATGAACTGAT CGACAGGATG AACGTGCTAG AACAGCTCAC CGCACTGATG
    CTGCCCTGGG GCTGTGACGT GCAAGGCAGC CTGAGCAGCC TGTTGAAACA TTTGGAGGAG
    GTCCCACAAC TCGTCAAGCT TGGGTTGAAA AACTGGAGAC TCACAGATAC AGAGATTAGA
    ATTTTAGGTG CATTTTTTGG AAAGAACCCT CTGAAAAACT TCCAGCAGTT GAATTTGGCG
    GGAAATCGTG TGAGCAGTGA TGGATGGCTT GCCTTCATGG GTGTATTTGA GAATCTTAAG
    CAATTAGTGT TTTTTGACTT TAGTACTAAA GAATTTCTAC CTGATCCAGC ATTAGTCAGA
    AAACTTAGCC AAGTGTTATC CAAGTTAACT TTTCTGCAAG AAGCTAGGCT TGTTGGGTGG
    CAATTTGATG ATGATGATCT CAGTGTTATT ACAGGTGCTT TTAAACTAGT AACTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001289433.1
    CDS276..1355
    Translation

    Target ORF information:

    RefSeq Version NM_001302504.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001302504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTGGTCTG ACTGACAGCT TGGGTAACTT GAAGAACCTT
    ACAAAGCTCA TAATGGATAA CATAAAGATG AATGAAGAAG ATGCTATAAA ACTAGCTGAA
    GGCCTGAAAA ACCTGAAGAA GATGTGTTTA TTTCATTTGA CCCACTTGTC TGACATTGGA
    GAGGGAATGG ATTACATAGT CAAGTCTCTG TCAAGTGAAC CCTGTGACCT TGAAGAAATT
    CAATTAGTCT CCTGCTGCTT GTCTGCAAAT GCAGTGAAAA TCCTAGCTCA GAATCTTCAC
    AATTTGGTCA AACTGAGCAT TCTTGATTTA TCAGAAAATT ACCTGGAAAA AGATGGAAAT
    GAAGCTCTTC ATGAACTGAT CGACAGGATG AACGTGCTAG AACAGCTCAC CGCACTGATG
    CTGCCCTGGG GCTGTGACGT GCAAGGCAGC CTGAGCAGCC TGTTGAAACA TTTGGAGGAG
    GTCCCACAAC TCGTCAAGCT TGGGTTGAAA AACTGGAGAC TCACAGATAC AGAGATTAGA
    ATTTTAGGTG CATTTTTTGG AAAGAACCCT CTGAAAAACT TCCAGCAGTT GAATTTGGCG
    GGAAATCGTG TGAGCAGTGA TGGATGGCTT GCCTTCATGG GTGTATTTGA GAATCTTAAG
    CAATTAGTGT TTTTTGACTT TAGTACTAAA GAATTTCTAC CTGATCCAGC ATTAGTCAGA
    AAACTTAGCC AAGTGTTATC CAAGTTAACT TTTCTGCAAG AAGCTAGGCT TGTTGGGTGG
    CAATTTGATG ATGATGATCT CAGTGTTATT ACAGGTGCTT TTAAACTAGT AACTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89747
    Clone ID Related Accession (Same CDS sequence) XM_017004619.1
    Accession Version XM_017004619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NLR family CARD domain-containing protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG ACAACTCTAG CCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860108.1
    CDS262..2628
    Translation

    Target ORF information:

    RefSeq Version XM_017004619.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG ACAACTCTAG CCTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16706
    Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
    Accession Version NM_021209.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product NLR family CARD domain-containing protein 4 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN998613.1, BC031555.1 and AW337918.1. On Nov 23, 2010 this sequence version replaced NM_021209.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY027787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_067032.3
    CDS276..3350
    Translation

    Target ORF information:

    RefSeq Version NM_021209.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021209.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16706
    Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
    Accession Version NM_001199139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product NLR family CARD domain-containing protein 4 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY035391.1 and AW337918.1. Transcript Variant: This variant (3) has an alternate splice site in the 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY035391.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186068.1
    CDS145..3219
    Translation

    Target ORF information:

    RefSeq Version NM_001199139.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16706
    Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
    Accession Version NM_001199138.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product NLR family CARD domain-containing protein 4 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK095467.1, BC031555.1 and AW337918.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK095467.1, SRR1660803.29064.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186067.1
    CDS262..3336
    Translation

    Target ORF information:

    RefSeq Version NM_001199138.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16706
    Clone ID Related Accession (Same CDS sequence) NM_021209.4 , NM_001199139.1 , NM_001199138.1 , NM_001199138.2
    Accession Version NM_001199138.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product NLR family CARD domain-containing protein 4 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121653.2, AK095467.1 and BC031555.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001199138.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031555.1, AK095467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000402280.6/ ENSP00000385428.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186067.1
    CDS268..3342
    Translation

    Target ORF information:

    RefSeq Version NM_001199138.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NLR family CARD domain containing 4 (NLRC4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199138.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATTTCA TAAAGGACAA TAGCCGAGCC CTTATTCAAA GAATGGGAAT GACTGTTATA 
    AAGCAAATCA CAGATGACCT ATTTGTATGG AATGTTCTGA ATCGCGAAGA AGTAAACATC
    ATTTGCTGCG AGAAGGTGGA GCAGGATGCT GCTAGAGGGA TCATTCACAT GATTTTGAAA
    AAGGGTTCAG AGTCCTGTAA CCTCTTTCTT AAATCCCTTA AGGAGTGGAA CTATCCTCTA
    TTTCAGGACT TGAATGGACA AAGTCTTTTT CATCAGACAT CAGAAGGAGA CTTGGACGAT
    TTGGCTCAGG ATTTAAAGGA CTTGTACCAT ACCCCATCTT TTCTGAACTT TTATCCCCTT
    GGTGAAGATA TTGACATTAT TTTTAACTTG AAAAGCACCT TCACAGAACC TGTCCTGTGG
    AGGAAGGACC AACACCATCA CCGCGTGGAG CAGCTGACCC TGAATGGCCT CCTGCAGGCT
    CTTCAGAGCC CCTGCATCAT TGAAGGGGAA TCTGGCAAAG GCAAGTCCAC TCTGCTGCAG
    CGAATTGCCA TGCTCTGGGG CTCCGGAAAG TGCAAGGCTC TGACCAAGTT CAAATTCGTC
    TTCTTCCTCC GTCTCAGCAG GGCCCAGGGT GGACTTTTTG AAACCCTCTG TGATCAACTC
    CTGGATATAC CTGGCACAAT CAGGAAGCAG ACATTCATGG CCATGCTGCT GAAGCTGCGG
    CAGAGGGTTC TTTTCCTTCT TGATGGCTAC AATGAATTCA AGCCCCAGAA CTGCCCAGAA
    ATCGAAGCCC TGATAAAGGA AAACCACCGC TTCAAGAACA TGGTCATCGT CACCACTACC
    ACTGAGTGCC TGAGGCACAT ACGGCAGTTT GGTGCCCTGA CTGCTGAGGT GGGGGATATG
    ACAGAAGACA GCGCCCAGGC TCTCATCCGA GAAGTGCTGA TCAAGGAGCT TGCTGAAGGC
    TTGTTGCTCC AAATTCAGAA ATCCAGGTGC TTGAGGAATC TCATGAAGAC CCCTCTCTTT
    GTGGTCATCA CTTGTGCAAT CCAGATGGGT GAAAGTGAGT TCCACTCTCA CACACAAACA
    ACGCTGTTCC ATACCTTCTA TGATCTGTTG ATACAGAAAA ACAAACACAA ACATAAAGGT
    GTGGCTGCAA GTGACTTCAT TCGGAGCCTG GACCACTGTG GAGACCTAGC TCTGGAGGGT
    GTGTTCTCCC ACAAGTTTGA TTTCGAACTG CAGGATGTGT CCAGCGTGAA TGAGGATGTC
    CTGCTGACAA CTGGGCTCCT CTGTAAATAT ACAGCTCAAA GGTTCAAGCC AAAGTATAAA
    TTCTTTCACA AGTCATTCCA GGAGTACACA GCAGGACGAA GACTCAGCAG TTTATTGACG
    TCTCATGAGC CAGAGGAGGT GACCAAGGGG AATGGTTACT TGCAGAAAAT GGTTTCCATT
    TCGGACATTA CATCCACTTA TAGCAGCCTG CTCCGGTACA CCTGTGGGTC ATCTGTGGAA
    GCCACCAGGG CTGTTATGAA GCACCTCGCA GCAGTGTATC AACACGGCTG CCTTCTCGGA
    CTTTCCATCG CCAAGAGGCC TCTCTGGAGA CAGGAATCTT TGCAAAGTGT GAAAAACACC
    ACTGAGCAAG AAATTCTGAA AGCCATAAAC ATCAATTCCT TTGTAGAGTG TGGCATCCAT
    TTATATCAAG AGAGTACATC CAAATCAGCC CTGAGCCAAG AATTTGAAGC TTTCTTTCAA
    GGTAAAAGCT TATATATCAA CTCAGGGAAC ATCCCCGATT ACTTATTTGA CTTCTTTGAA
    CATTTGCCCA ATTGTGCAAG TGCCCTGGAC TTCATTAAAC TGGACTTTTA TGGGGGAGCT
    ATGGCTTCAT GGGAAAAGGC TGCAGAAGAC ACAGGTGGAA TCCACATGGA AGAGGCCCCA
    GAAACCTACA TTCCCAGCAG GGCTGTATCT TTGTTCTTCA ACTGGAAGCA GGAATTCAGG
    ACTCTGGAGG TCACACTCCG GGATTTCAGC AAGTTGAATA AGCAAGATAT CAGATATCTG
    GGGAAAATAT TCAGCTCTGC CACAAGCCTC AGGCTGCAAA TAAAGAGATG TGCTGGTGTG
    GCTGGAAGCC TCAGTTTGGT CCTCAGCACC TGTAAGAACA TTTATTCTCT CATGGTGGAA
    GCCAGTCCCC TCACCATAGA AGATGAGAGG CACATCACAT CTGTAACAAA CCTGAAAACC
    TTGAGTATTC ATGACCTACA GAATCAACGG CTGCCGGGTG GTCTGACTGA CAGCTTGGGT
    AACTTGAAGA ACCTTACAAA GCTCATAATG GATAACATAA AGATGAATGA AGAAGATGCT
    ATAAAACTAG CTGAAGGCCT GAAAAACCTG AAGAAGATGT GTTTATTTCA TTTGACCCAC
    TTGTCTGACA TTGGAGAGGG AATGGATTAC ATAGTCAAGT CTCTGTCAAG TGAACCCTGT
    GACCTTGAAG AAATTCAATT AGTCTCCTGC TGCTTGTCTG CAAATGCAGT GAAAATCCTA
    GCTCAGAATC TTCACAATTT GGTCAAACTG AGCATTCTTG ATTTATCAGA AAATTACCTG
    GAAAAAGATG GAAATGAAGC TCTTCATGAA CTGATCGACA GGATGAACGT GCTAGAACAG
    CTCACCGCAC TGATGCTGCC CTGGGGCTGT GACGTGCAAG GCAGCCTGAG CAGCCTGTTG
    AAACATTTGG AGGAGGTCCC ACAACTCGTC AAGCTTGGGT TGAAAAACTG GAGACTCACA
    GATACAGAGA TTAGAATTTT AGGTGCATTT TTTGGAAAGA ACCCTCTGAA AAACTTCCAG
    CAGTTGAATT TGGCGGGAAA TCGTGTGAGC AGTGATGGAT GGCTTGCCTT CATGGGTGTA
    TTTGAGAATC TTAAGCAATT AGTGTTTTTT GACTTTAGTA CTAAAGAATT TCTACCTGAT
    CCAGCATTAG TCAGAAAACT TAGCCAAGTG TTATCCAAGT TAACTTTTCT GCAAGAAGCT
    AGGCTTGTTG GGTGGCAATT TGATGATGAT GATCTCAGTG TTATTACAGG TGCTTTTAAA
    CTAGTAACTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.