PYGM cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGM
Entrez Gene ID	5837
Full Name	glycogen phosphorylase, muscle associated
General protein information	Preferred Names glycogen phosphorylase, muscle associated Names glycogen phosphorylase, muscle form myophosphorylase phosphorylase, glycogen, muscle
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009].
Disorder MIM:	608455
Disorder Html:	McArdle disease, 232600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005609.3	NP_005600.1	glycogen phosphorylase, muscle form isoform 1
NM_005609.4	NP_005600.1	glycogen phosphorylase, muscle form isoform 1
NM_001164716.1	NP_001158188.1	glycogen phosphorylase, muscle form isoform 2
NM_005609.2	NP_005600.1	glycogen phosphorylase, muscle form isoform 1

Pathways from BioSystems

Homologies

	PYGM	XP_001114805.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007939	XP_317541.3
Oryza sativa (rice)	Os01g0851700	NP_001044823.1
Homo sapiens (human)	PYGM	NP_005600.1
Canis lupus familiaris (dog)	PYGM	XP_853123.1
Rattus norvegicus (Norway rat)	Pygm	NP_036770.1
Drosophila melanogaster (fruit fly)	GlyP	NP_722762.1
Xenopus tropicalis (tropical clawed frog)	LOC100491051	XP_002935410.2
Arabidopsis thaliana (thale cress)	PHS2	NP_190281.1
Bos taurus (cattle)	PYGM	NP_786980.1
Mus musculus (house mouse)	Pygm	NP_035354.1
Pan troglodytes (chimpanzee)	PYGM	XP_001165783.2
Danio rerio (zebrafish)	pygma	NP_001018464.1
Caenorhabditis elegans (roundworm)	T22F3.3	NP_504007.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel variant in the PYGM gene causing late-onset limb-girdle myopathy, ptosis, and camptocormia.
Ch?raud C, Froissart R, Lannes B, Echaniz-Laguna A
Muscle & nerve57(1)157-160(2018 Jan)

The glial phosphorylase of glycogen isoform is reduced in the dorsolateral prefrontal cortex in chronic schizophrenia.
Pinacho R, Vila E, Prades R, Tarrag? T, Castro E, Ferrer I, Ramos B
Schizophrenia research177(1-3)37-43(2016 11)

Taking advantage of an old concept, "illegitimate transcription", for a proposed novel method of genetic diagnosis of McArdle disease.
Garcia-Consuegra I, Bl?zquez A, Rubio JC, Arenas J, Ballester-Lopez A, Gonz?lez-Quintana A, Andreu AL, Pin?s T, Coll-Cant? J, Lucia A, Nogales-Gadea G, Mart?n MA
Genetics in medicine : official journal of the American College of Medical Genetics18(11)1128-1135(2016 Nov)

Genetic evaluation of AMPD1, CPT2, and PGYM metabolic enzymes in patients with chronic fatigue syndrome.
Maltese PE, Venturini L, Poplavskaya E, Bertelli M, Cecchin S, Granato M, Nikulina SY, Salmina A, Aksyutina N, Capelli E, Ricevuti G, Lorusso L
Genetics and molecular research : GMR15(3)(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This report expands the phenotype and genotype of McArdle disease and suggests that PYGM mutations should be looked for in patients with very late-onset myopathy with no previous history of exercise intolerance
Title: Novel variant in the PYGM gene causing late-onset limb-girdle myopathy, ptosis, and camptocormia.

Results show that PYGM and RAC1 are altered in the dorsolateral prefrontal cortex in chronic schizophrenia and are controlled by NMDA signaling in the rodent cortex and cortical astrocytes suggesting an altered NMDA-dependent glycogenolysis in astrocytes in schizophrenia.
Title: The glial phosphorylase of glycogen isoform is reduced in the dorsolateral prefrontal cortex in chronic schizophrenia.

Because the NMD mechanism does not seem to operate in nonspecific cells, PBMCs were more suitable than muscle biopsies for detecting the pathogenicity of some PYGM mutations, notably the silent mutation whose effect in the splicing of intron 6 was unnoticed in previous muscle transcriptomic studies
Title: Taking advantage of an old concept, "illegitimate transcription", for a proposed novel method of genetic diagnosis of McArdle disease.

Variations in AMPD1, CPT2, and PGYM genes are not associated with the onset, susceptibility, or severity of chronic fatigue syndrome.
Title: Genetic evaluation of AMPD1, CPT2, and PGYM metabolic enzymes in patients with chronic fatigue syndrome.

update of the reported mutations and polymorphisms in the PYGM gene [review]
Title: McArdle Disease: Update of Reported Mutations and Polymorphisms in the PYGM Gene.

The following PYGM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26256	NM_005609.4 Latest version!	Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22767	NM_001164716.1 Latest version!	Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
View Old Accession Versions >>
OHu26256	NM_005609.3	Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26256	NM_005609.2	Homo sapiens phosphorylase, glycogen, muscle (PYGM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYGM ( NM_005609.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005600.1 Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26256
Clone ID Related Accession (Same CDS sequence)	NM_005609.3 , NM_005609.4 , NM_005609.2
Accession Version	NM_005609.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, muscle form isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC130514.1 and AJ572752.1. This sequence is a reference standard in the RefSeqGene project. On Jun 16, 2016 this sequence version replaced NM_005609.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126392.1, SRR1660809.134847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTAGAGTTC TATATGGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCTTAGAGAA TGCCTGTGAC GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTGG AGGAAATTGA GGAGGATGCG GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TTCTTGACTC CATGGCAACA CTGGGCCTGG CCGCCTATGG CTACGGGATT 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCCGGGG GCTGGCAGAT GGAGGAGGCC 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCCGAGTT CACGCTACCT 
GTGCACTTCT ACGGCCATGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTGGTACTGG CCATGCCCTA CGATACGCCC GTGCCTGGCT ATCGCAACAA TGTTGTCAAC 
ACCATGCGCC TCTGGTCTGC CAAGGCTCCC AATGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCTGT GTTGGACCGA AACCTGGCGG AGAACATCTC TCGTGTCCTG 
TACCCCAATG ATAATTTCTT CGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTATTTCGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGT CGCTTCAAGT CTTCCAAGTT CGGCTGCCGT 
GATCCCGTGC GCACGAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCCTGGCCAT CCCCGAGCTG ATGAGGATCC TGGTGGACCT GGAACGGATG 
GACTGGGACA AGGCGTGGGA TGTGACAGTG AGGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCGGTG CACCTCTTGG AGACGCTGCT GCCGCGGCAC 
CTCCAGATCA TCTACGAGAT CAACCAGCGC TTCCTCAACC GGGTGGCGGC CGCATTCCCA 
GGGGACGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCAGT GAAGCGCATC 
AACATGGCAC ACCTGTGCAT CGCGGGGTCG CACGCCGTCA ACGGCGTGGC GCGCATCCAC 
TCCGAGATCC TCAAGAAGAC CATCTTCAAA GACTTCTATG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAACGGCAT CACCCCTCGG CGCTGGCTGG TTCTGTGTAA CCCCGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATCGGGGAG GACTTCATCT CTGACCTGGA CCAGCTGCGC 
AAACTGCTCT CCTTTGTGGA TGATGAAGCT TTCATTCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TGAAGTTTGC TGCCTACCTA GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGACATCCA GGTGAAGCGG ATTCACGAAT ATAAACGACA GCTCCTCAAC 
TGCCTCCATG TCATCACCCT GTACAACCGC ATCAAGAGGG AGCCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAGACTCG TCACAGCCAT CGGGGATGTG GTCAACCATG ACCCGGCAGT GGGTGACCGC 
CTCCGTGTCA TCTTCCTGGA GAACTACCGA GTCTCACTGG CCGAGAAAGT GATCCCAGCT 
GCAGACCTCT CTGAGCAGAT CTCCACTGCG GGCACTGAAG CCTCAGGCAC CGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA TGTGGAGATG 
GCAGAAGAGG CGGGAGAGGA AAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGATGTGGAT 
AAGCTTGACC AAAGAGGGTA CAATGCCCAG GAGTACTACG ATCGCATTCC TGAGCTTCGG 
CAGGTCATTG AGCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCCGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTCGCAGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC TTGTACAAGA ACCCAAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC CACCTCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTGGAGCCT TCCCGCCAGC GCCTGCCAGC CCCGGATGAG 
GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005600.1
CDS	400..2928
Translation	MSRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKISGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGHVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERMDWDKAWDVTVRTCAYTNHTVLPEALERWPVHLLETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEAI

Target ORF information:

RefSeq Version	NM_005609.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005609.3

ORF Insert Sequence:

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTAGAGTTC TATATGGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCTTAGAGAA TGCCTGTGAC GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTGG AGGAAATTGA GGAGGATGCG GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TTCTTGACTC CATGGCAACA CTGGGCCTGG CCGCCTATGG CTACGGGATT 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCCGGGG GCTGGCAGAT GGAGGAGGCC 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCCGAGTT CACGCTACCT 
GTGCACTTCT ACGGCCATGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTGGTACTGG CCATGCCCTA CGATACGCCC GTGCCTGGCT ATCGCAACAA TGTTGTCAAC 
ACCATGCGCC TCTGGTCTGC CAAGGCTCCC AATGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCTGT GTTGGACCGA AACCTGGCGG AGAACATCTC TCGTGTCCTG 
TACCCCAATG ATAATTTCTT CGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTATTTCGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGT CGCTTCAAGT CTTCCAAGTT CGGCTGCCGT 
GATCCCGTGC GCACGAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCCTGGCCAT CCCCGAGCTG ATGAGGATCC TGGTGGACCT GGAACGGATG 
GACTGGGACA AGGCGTGGGA TGTGACAGTG AGGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCGGTG CACCTCTTGG AGACGCTGCT GCCGCGGCAC 
CTCCAGATCA TCTACGAGAT CAACCAGCGC TTCCTCAACC GGGTGGCGGC CGCATTCCCA 
GGGGACGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCAGT GAAGCGCATC 
AACATGGCAC ACCTGTGCAT CGCGGGGTCG CACGCCGTCA ACGGCGTGGC GCGCATCCAC 
TCCGAGATCC TCAAGAAGAC CATCTTCAAA GACTTCTATG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAACGGCAT CACCCCTCGG CGCTGGCTGG TTCTGTGTAA CCCCGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATCGGGGAG GACTTCATCT CTGACCTGGA CCAGCTGCGC 
AAACTGCTCT CCTTTGTGGA TGATGAAGCT TTCATTCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TGAAGTTTGC TGCCTACCTA GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGACATCCA GGTGAAGCGG ATTCACGAAT ATAAACGACA GCTCCTCAAC 
TGCCTCCATG TCATCACCCT GTACAACCGC ATCAAGAGGG AGCCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAGACTCG TCACAGCCAT CGGGGATGTG GTCAACCATG ACCCGGCAGT GGGTGACCGC 
CTCCGTGTCA TCTTCCTGGA GAACTACCGA GTCTCACTGG CCGAGAAAGT GATCCCAGCT 
GCAGACCTCT CTGAGCAGAT CTCCACTGCG GGCACTGAAG CCTCAGGCAC CGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA TGTGGAGATG 
GCAGAAGAGG CGGGAGAGGA AAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGATGTGGAT 
AAGCTTGACC AAAGAGGGTA CAATGCCCAG GAGTACTACG ATCGCATTCC TGAGCTTCGG 
CAGGTCATTG AGCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCCGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTCGCAGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC TTGTACAAGA ACCCAAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC CACCTCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTGGAGCCT TCCCGCCAGC GCCTGCCAGC CCCGGATGAG 
GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGM ( NM_005609.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005600.1 Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26256
Clone ID Related Accession (Same CDS sequence)	NM_005609.3 , NM_005609.4 , NM_005609.2
Accession Version	NM_005609.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, muscle form isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC130514.1 and AJ572752.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005609.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.219881.1, SRR1803611.188289.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000164139.4/ ENSP00000164139.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTAGAGTTC TATATGGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCTTAGAGAA TGCCTGTGAC GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTGG AGGAAATTGA GGAGGATGCG GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TTCTTGACTC CATGGCAACA CTGGGCCTGG CCGCCTATGG CTACGGGATT 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCCGGGG GCTGGCAGAT GGAGGAGGCC 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCCGAGTT CACGCTACCT 
GTGCACTTCT ACGGCCATGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTGGTACTGG CCATGCCCTA CGATACGCCC GTGCCTGGCT ATCGCAACAA TGTTGTCAAC 
ACCATGCGCC TCTGGTCTGC CAAGGCTCCC AATGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCTGT GTTGGACCGA AACCTGGCGG AGAACATCTC TCGTGTCCTG 
TACCCCAATG ATAATTTCTT CGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTATTTCGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGT CGCTTCAAGT CTTCCAAGTT CGGCTGCCGT 
GATCCCGTGC GCACGAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCCTGGCCAT CCCCGAGCTG ATGAGGATCC TGGTGGACCT GGAACGGATG 
GACTGGGACA AGGCGTGGGA TGTGACAGTG AGGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCGGTG CACCTCTTGG AGACGCTGCT GCCGCGGCAC 
CTCCAGATCA TCTACGAGAT CAACCAGCGC TTCCTCAACC GGGTGGCGGC CGCATTCCCA 
GGGGACGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCAGT GAAGCGCATC 
AACATGGCAC ACCTGTGCAT CGCGGGGTCG CACGCCGTCA ACGGCGTGGC GCGCATCCAC 
TCCGAGATCC TCAAGAAGAC CATCTTCAAA GACTTCTATG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAACGGCAT CACCCCTCGG CGCTGGCTGG TTCTGTGTAA CCCCGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATCGGGGAG GACTTCATCT CTGACCTGGA CCAGCTGCGC 
AAACTGCTCT CCTTTGTGGA TGATGAAGCT TTCATTCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TGAAGTTTGC TGCCTACCTA GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGACATCCA GGTGAAGCGG ATTCACGAAT ATAAACGACA GCTCCTCAAC 
TGCCTCCATG TCATCACCCT GTACAACCGC ATCAAGAGGG AGCCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAGACTCG TCACAGCCAT CGGGGATGTG GTCAACCATG ACCCGGCAGT GGGTGACCGC 
CTCCGTGTCA TCTTCCTGGA GAACTACCGA GTCTCACTGG CCGAGAAAGT GATCCCAGCT 
GCAGACCTCT CTGAGCAGAT CTCCACTGCG GGCACTGAAG CCTCAGGCAC CGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA TGTGGAGATG 
GCAGAAGAGG CGGGAGAGGA AAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGATGTGGAT 
AAGCTTGACC AAAGAGGGTA CAATGCCCAG GAGTACTACG ATCGCATTCC TGAGCTTCGG 
CAGGTCATTG AGCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCCGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTCGCAGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC TTGTACAAGA ACCCAAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC CACCTCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTGGAGCCT TCCCGCCAGC GCCTGCCAGC CCCGGATGAG 
GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005600.1
CDS	77..2605
Translation	MSRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKISGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGHVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERMDWDKAWDVTVRTCAYTNHTVLPEALERWPVHLLETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEAI

Target ORF information:

RefSeq Version	NM_005609.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005609.4

ORF Insert Sequence:

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTAGAGTTC TATATGGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCTTAGAGAA TGCCTGTGAC GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTGG AGGAAATTGA GGAGGATGCG GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TTCTTGACTC CATGGCAACA CTGGGCCTGG CCGCCTATGG CTACGGGATT 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCCGGGG GCTGGCAGAT GGAGGAGGCC 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCCGAGTT CACGCTACCT 
GTGCACTTCT ACGGCCATGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTGGTACTGG CCATGCCCTA CGATACGCCC GTGCCTGGCT ATCGCAACAA TGTTGTCAAC 
ACCATGCGCC TCTGGTCTGC CAAGGCTCCC AATGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCTGT GTTGGACCGA AACCTGGCGG AGAACATCTC TCGTGTCCTG 
TACCCCAATG ATAATTTCTT CGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTATTTCGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGT CGCTTCAAGT CTTCCAAGTT CGGCTGCCGT 
GATCCCGTGC GCACGAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCCTGGCCAT CCCCGAGCTG ATGAGGATCC TGGTGGACCT GGAACGGATG 
GACTGGGACA AGGCGTGGGA TGTGACAGTG AGGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCGGTG CACCTCTTGG AGACGCTGCT GCCGCGGCAC 
CTCCAGATCA TCTACGAGAT CAACCAGCGC TTCCTCAACC GGGTGGCGGC CGCATTCCCA 
GGGGACGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCAGT GAAGCGCATC 
AACATGGCAC ACCTGTGCAT CGCGGGGTCG CACGCCGTCA ACGGCGTGGC GCGCATCCAC 
TCCGAGATCC TCAAGAAGAC CATCTTCAAA GACTTCTATG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAACGGCAT CACCCCTCGG CGCTGGCTGG TTCTGTGTAA CCCCGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATCGGGGAG GACTTCATCT CTGACCTGGA CCAGCTGCGC 
AAACTGCTCT CCTTTGTGGA TGATGAAGCT TTCATTCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TGAAGTTTGC TGCCTACCTA GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGACATCCA GGTGAAGCGG ATTCACGAAT ATAAACGACA GCTCCTCAAC 
TGCCTCCATG TCATCACCCT GTACAACCGC ATCAAGAGGG AGCCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAGACTCG TCACAGCCAT CGGGGATGTG GTCAACCATG ACCCGGCAGT GGGTGACCGC 
CTCCGTGTCA TCTTCCTGGA GAACTACCGA GTCTCACTGG CCGAGAAAGT GATCCCAGCT 
GCAGACCTCT CTGAGCAGAT CTCCACTGCG GGCACTGAAG CCTCAGGCAC CGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA TGTGGAGATG 
GCAGAAGAGG CGGGAGAGGA AAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGATGTGGAT 
AAGCTTGACC AAAGAGGGTA CAATGCCCAG GAGTACTACG ATCGCATTCC TGAGCTTCGG 
CAGGTCATTG AGCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCCGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTCGCAGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC TTGTACAAGA ACCCAAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC CACCTCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTGGAGCCT TCCCGCCAGC GCCTGCCAGC CCCGGATGAG 
GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGM ( NM_005609.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005600.1 Homo sapiens phosphorylase, glycogen, muscle (PYGM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26256
Clone ID Related Accession (Same CDS sequence)	NM_005609.3 , NM_005609.4 , NM_005609.2
Accession Version	NM_005609.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	glycogen phosphorylase, muscle form isoform 1
Comment

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTAGAGTTC TATATGGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCTTAGAGAA TGCCTGTGAC GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTGG AGGAAATTGA GGAGGATGCG GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TTCTTGACTC CATGGCAACA CTGGGCCTGG CCGCCTATGG CTACGGGATT 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCCGGGG GCTGGCAGAT GGAGGAGGCC 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCCGAGTT CACGCTACCT 
GTGCACTTCT ACGGCCATGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTGGTACTGG CCATGCCCTA CGATACGCCC GTGCCTGGCT ATCGCAACAA TGTTGTCAAC 
ACCATGCGCC TCTGGTCTGC CAAGGCTCCC AATGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCTGT GTTGGACCGA AACCTGGCGG AGAACATCTC TCGTGTCCTG 
TACCCCAATG ATAATTTCTT CGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTATTTCGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGT CGCTTCAAGT CTTCCAAGTT CGGCTGCCGT 
GATCCCGTGC GCACGAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCCTGGCCAT CCCCGAGCTG ATGAGGATCC TGGTGGACCT GGAACGGATG 
GACTGGGACA AGGCGTGGGA TGTGACAGTG AGGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCGGTG CACCTCTTGG AGACGCTGCT GCCGCGGCAC 
CTCCAGATCA TCTACGAGAT CAACCAGCGC TTCCTCAACC GGGTGGCGGC CGCATTCCCA 
GGGGACGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCAGT GAAGCGCATC 
AACATGGCAC ACCTGTGCAT CGCGGGGTCG CACGCCGTCA ACGGCGTGGC GCGCATCCAC 
TCCGAGATCC TCAAGAAGAC CATCTTCAAA GACTTCTATG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAACGGCAT CACCCCTCGG CGCTGGCTGG TTCTGTGTAA CCCCGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATCGGGGAG GACTTCATCT CTGACCTGGA CCAGCTGCGC 
AAACTGCTCT CCTTTGTGGA TGATGAAGCT TTCATTCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TGAAGTTTGC TGCCTACCTA GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGACATCCA GGTGAAGCGG ATTCACGAAT ATAAACGACA GCTCCTCAAC 
TGCCTCCATG TCATCACCCT GTACAACCGC ATCAAGAGGG AGCCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAGACTCG TCACAGCCAT CGGGGATGTG GTCAACCATG ACCCGGCAGT GGGTGACCGC 
CTCCGTGTCA TCTTCCTGGA GAACTACCGA GTCTCACTGG CCGAGAAAGT GATCCCAGCT 
GCAGACCTCT CTGAGCAGAT CTCCACTGCG GGCACTGAAG CCTCAGGCAC CGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA TGTGGAGATG 
GCAGAAGAGG CGGGAGAGGA AAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGATGTGGAT 
AAGCTTGACC AAAGAGGGTA CAATGCCCAG GAGTACTACG ATCGCATTCC TGAGCTTCGG 
CAGGTCATTG AGCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCCGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTCGCAGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC TTGTACAAGA ACCCAAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC CACCTCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTGGAGCCT TCCCGCCAGC GCCTGCCAGC CCCGGATGAG 
GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_005600.1

Translation

MSRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKISGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGHVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERMDWDKAWDVTVRTCAYTNHTVLPEALERWPVHLLETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEAI

Target ORF information:

RefSeq Version	NM_005609.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphorylase, glycogen, muscle (PYGM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005609.2

ORF Insert Sequence:

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAGGATCT ACTACCTGTC TTTAGAGTTC TATATGGGAC GGACGCTACA GAACACCATG 
GTGAACCTGG CCTTAGAGAA TGCCTGTGAC GAGGCCACCT ACCAGCTGGG CCTGGACATG 
GAGGAGCTGG AGGAAATTGA GGAGGATGCG GGGCTGGGCA ACGGGGGCCT GGGCCGGCTG 
GCAGCCTGCT TTCTTGACTC CATGGCAACA CTGGGCCTGG CCGCCTATGG CTACGGGATT 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATCTCCGGGG GCTGGCAGAT GGAGGAGGCC 
GATGACTGGC TTCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCCGAGTT CACGCTACCT 
GTGCACTTCT ACGGCCATGT GGAGCACACC AGCCAGGGTG CCAAGTGGGT GGACACACAG 
GTGGTACTGG CCATGCCCTA CGATACGCCC GTGCCTGGCT ATCGCAACAA TGTTGTCAAC 
ACCATGCGCC TCTGGTCTGC CAAGGCTCCC AATGACTTCA ACCTCAAGGA CTTCAATGTC 
GGTGGCTACA TCCAGGCTGT GTTGGACCGA AACCTGGCGG AGAACATCTC TCGTGTCCTG 
TACCCCAATG ATAATTTCTT CGAAGGGAAG GAGCTGCGGC TGAAGCAGGA GTATTTCGTG 
GTGGCTGCCA CCCTCCAGGA CATCATCCGT CGCTTCAAGT CTTCCAAGTT CGGCTGCCGT 
GATCCCGTGC GCACGAACTT CGATGCCTTC CCAGATAAGG TGGCCATCCA GCTCAATGAC 
ACCCACCCCT CCCTGGCCAT CCCCGAGCTG ATGAGGATCC TGGTGGACCT GGAACGGATG 
GACTGGGACA AGGCGTGGGA TGTGACAGTG AGGACCTGTG CCTACACCAA CCACACGGTG 
CTGCCCGAGG CCCTGGAGCG CTGGCCGGTG CACCTCTTGG AGACGCTGCT GCCGCGGCAC 
CTCCAGATCA TCTACGAGAT CAACCAGCGC TTCCTCAACC GGGTGGCGGC CGCATTCCCA 
GGGGACGTAG ACCGGCTGCG GCGCATGTCG CTGGTGGAGG AGGGCGCAGT GAAGCGCATC 
AACATGGCAC ACCTGTGCAT CGCGGGGTCG CACGCCGTCA ACGGCGTGGC GCGCATCCAC 
TCCGAGATCC TCAAGAAGAC CATCTTCAAA GACTTCTATG AGCTGGAGCC TCATAAGTTC 
CAGAATAAGA CCAACGGCAT CACCCCTCGG CGCTGGCTGG TTCTGTGTAA CCCCGGGCTG 
GCAGAGGTCA TTGCTGAGCG CATCGGGGAG GACTTCATCT CTGACCTGGA CCAGCTGCGC 
AAACTGCTCT CCTTTGTGGA TGATGAAGCT TTCATTCGGG ATGTGGCCAA AGTGAAGCAG 
GAAAACAAGT TGAAGTTTGC TGCCTACCTA GAGAGGGAAT ACAAAGTCCA CATCAACCCC 
AACTCACTCT TCGACATCCA GGTGAAGCGG ATTCACGAAT ATAAACGACA GCTCCTCAAC 
TGCCTCCATG TCATCACCCT GTACAACCGC ATCAAGAGGG AGCCCAATAA GTTTTTTGTG 
CCTCGGACTG TGATGATTGG AGGGAAGGCT GCACCTGGGT ACCACATGGC CAAGATGATC 
ATCAGACTCG TCACAGCCAT CGGGGATGTG GTCAACCATG ACCCGGCAGT GGGTGACCGC 
CTCCGTGTCA TCTTCCTGGA GAACTACCGA GTCTCACTGG CCGAGAAAGT GATCCCAGCT 
GCAGACCTCT CTGAGCAGAT CTCCACTGCG GGCACTGAAG CCTCAGGCAC CGGCAACATG 
AAGTTCATGC TCAACGGGGC TCTGACCATT GGCACCATGG ACGGGGCCAA TGTGGAGATG 
GCAGAAGAGG CGGGAGAGGA AAACTTCTTC ATCTTTGGCA TGCGGGTGGA GGATGTGGAT 
AAGCTTGACC AAAGAGGGTA CAATGCCCAG GAGTACTACG ATCGCATTCC TGAGCTTCGG 
CAGGTCATTG AGCAGCTGAG CAGTGGCTTC TTCTCCCCCA AACAGCCCGA CCTGTTCAAG 
GACATTGTCA ATATGCTCAT GCACCATGAC CGGTTTAAAG TCTTCGCAGA TTATGAAGAC 
TACATTAAAT GCCAGGAGAA AGTCAGCGCC TTGTACAAGA ACCCAAGAGA GTGGACGCGG 
ATGGTGATCC GGAACATAGC CACCTCTGGC AAGTTCTCCA GTGACCGCAC CATTGCCCAG 
TATGCCCGGG AGATCTGGGG TGTGGAGCCT TCCCGCCAGC GCCTGCCAGC CCCGGATGAG 
GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGM ( NM_001164716.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001158188.1 Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22767
Clone ID Related Accession (Same CDS sequence)	NM_001164716.1
Accession Version	NM_001164716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, muscle form isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001462.3, AK056607.1 and BM987822.1. Transcript Variant: This variant (2) lacks two exons uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056607.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAAGATCT CCGGGGGCTG GCAGATGGAG GAGGCCGATG ACTGGCTTCG CTACGGCAAC 
CCCTGGGAGA AGGCCCGGCC CGAGTTCACG CTACCTGTGC ACTTCTACGG CCATGTGGAG 
CACACCAGCC AGGGTGCCAA GTGGGTGGAC ACACAGGTGG TACTGGCCAT GCCCTACGAT 
ACGCCCGTGC CTGGCTATCG CAACAATGTT GTCAACACCA TGCGCCTCTG GTCTGCCAAG 
GCTCCCAATG ACTTCAACCT CAAGGACTTC AATGTCGGTG GCTACATCCA GGCTGTGTTG 
GACCGAAACC TGGCGGAGAA CATCTCTCGT GTCCTGTACC CCAATGATAA TTTCTTCGAA 
GGGAAGGAGC TGCGGCTGAA GCAGGAGTAT TTCGTGGTGG CTGCCACCCT CCAGGACATC 
ATCCGTCGCT TCAAGTCTTC CAAGTTCGGC TGCCGTGATC CCGTGCGCAC GAACTTCGAT 
GCCTTCCCAG ATAAGGTGGC CATCCAGCTC AATGACACCC ACCCCTCCCT GGCCATCCCC 
GAGCTGATGA GGATCCTGGT GGACCTGGAA CGGATGGACT GGGACAAGGC GTGGGATGTG 
ACAGTGAGGA CCTGTGCCTA CACCAACCAC ACGGTGCTGC CCGAGGCCCT GGAGCGCTGG 
CCGGTGCACC TCTTGGAGAC GCTGCTGCCG CGGCACCTCC AGATCATCTA CGAGATCAAC 
CAGCGCTTCC TCAACCGGGT GGCGGCCGCA TTCCCAGGGG ACGTAGACCG GCTGCGGCGC 
ATGTCGCTGG TGGAGGAGGG CGCAGTGAAG CGCATCAACA TGGCACACCT GTGCATCGCG 
GGGTCGCACG CCGTCAACGG CGTGGCGCGC ATCCACTCCG AGATCCTCAA GAAGACCATC 
TTCAAAGACT TCTATGAGCT GGAGCCTCAT AAGTTCCAGA ATAAGACCAA CGGCATCACC 
CCTCGGCGCT GGCTGGTTCT GTGTAACCCC GGGCTGGCAG AGGTCATTGC TGAGCGCATC 
GGGGAGGACT TCATCTCTGA CCTGGACCAG CTGCGCAAAC TGCTCTCCTT TGTGGATGAT 
GAAGCTTTCA TTCGGGATGT GGCCAAAGTG AAGCAGGAAA ACAAGTTGAA GTTTGCTGCC 
TACCTAGAGA GGGAATACAA AGTCCACATC AACCCCAACT CACTCTTCGA CATCCAGGTG 
AAGCGGATTC ACGAATATAA ACGACAGCTC CTCAACTGCC TCCATGTCAT CACCCTGTAC 
AACCGCATCA AGAGGGAGCC CAATAAGTTT TTTGTGCCTC GGACTGTGAT GATTGGAGGG 
AAGGCTGCAC CTGGGTACCA CATGGCCAAG ATGATCATCA GACTCGTCAC AGCCATCGGG 
GATGTGGTCA ACCATGACCC GGCAGTGGGT GACCGCCTCC GTGTCATCTT CCTGGAGAAC 
TACCGAGTCT CACTGGCCGA GAAAGTGATC CCAGCTGCAG ACCTCTCTGA GCAGATCTCC 
ACTGCGGGCA CTGAAGCCTC AGGCACCGGC AACATGAAGT TCATGCTCAA CGGGGCTCTG 
ACCATTGGCA CCATGGACGG GGCCAATGTG GAGATGGCAG AAGAGGCGGG AGAGGAAAAC 
TTCTTCATCT TTGGCATGCG GGTGGAGGAT GTGGATAAGC TTGACCAAAG AGGGTACAAT 
GCCCAGGAGT ACTACGATCG CATTCCTGAG CTTCGGCAGG TCATTGAGCA GCTGAGCAGT 
GGCTTCTTCT CCCCCAAACA GCCCGACCTG TTCAAGGACA TTGTCAATAT GCTCATGCAC 
CATGACCGGT TTAAAGTCTT CGCAGATTAT GAAGACTACA TTAAATGCCA GGAGAAAGTC 
AGCGCCTTGT ACAAGAACCC AAGAGAGTGG ACGCGGATGG TGATCCGGAA CATAGCCACC 
TCTGGCAAGT TCTCCAGTGA CCGCACCATT GCCCAGTATG CCCGGGAGAT CTGGGGTGTG 
GAGCCTTCCC GCCAGCGCCT GCCAGCCCCG GATGAGGCCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158188.1
CDS	818..3082
Translation	MSRPLSDQEKRKQISVRGLAGVENVTELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYEKDPKKISGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGHVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTNFDAFPDKVAIQLNDTHPSLAIPELMRILVDLERMDWDKAWDVTVRTCAYTNHTVLPEALERWPVHLLETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDFISDLDQLRKLLSFVDDEAFIRDVAKVKQENKLKFAAYLEREYKVHINPNSLFDIQVKRIHEYKRQLLNCLHVITLYNRIKREPNKFFVPRTVMIGGKAAPGYHMAKMIIRLVTAIGDVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVDKLDQRGYNAQEYYDRIPELRQVIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADYEDYIKCQEKVSALYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEAI

Target ORF information:

RefSeq Version	NM_001164716.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase, muscle associated (PYGM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164716.1

ORF Insert Sequence:

ATGTCCCGGC CCCTGTCAGA CCAAGAGAAA AGAAAGCAAA TCAGTGTGCG TGGCCTGGCC 
GGCGTGGAGA ACGTGACTGA GCTGAAAAAG AACTTCAACC GGCACCTGCA TTTCACACTC 
GTAAAGGACC GCAATGTGGC CACCCCACGA GACTACTACT TTGCTCTGGC CCATACCGTG 
CGCGACCACC TCGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTATGA GAAGGACCCC 
AAGAAGATCT CCGGGGGCTG GCAGATGGAG GAGGCCGATG ACTGGCTTCG CTACGGCAAC 
CCCTGGGAGA AGGCCCGGCC CGAGTTCACG CTACCTGTGC ACTTCTACGG CCATGTGGAG 
CACACCAGCC AGGGTGCCAA GTGGGTGGAC ACACAGGTGG TACTGGCCAT GCCCTACGAT 
ACGCCCGTGC CTGGCTATCG CAACAATGTT GTCAACACCA TGCGCCTCTG GTCTGCCAAG 
GCTCCCAATG ACTTCAACCT CAAGGACTTC AATGTCGGTG GCTACATCCA GGCTGTGTTG 
GACCGAAACC TGGCGGAGAA CATCTCTCGT GTCCTGTACC CCAATGATAA TTTCTTCGAA 
GGGAAGGAGC TGCGGCTGAA GCAGGAGTAT TTCGTGGTGG CTGCCACCCT CCAGGACATC 
ATCCGTCGCT TCAAGTCTTC CAAGTTCGGC TGCCGTGATC CCGTGCGCAC GAACTTCGAT 
GCCTTCCCAG ATAAGGTGGC CATCCAGCTC AATGACACCC ACCCCTCCCT GGCCATCCCC 
GAGCTGATGA GGATCCTGGT GGACCTGGAA CGGATGGACT GGGACAAGGC GTGGGATGTG 
ACAGTGAGGA CCTGTGCCTA CACCAACCAC ACGGTGCTGC CCGAGGCCCT GGAGCGCTGG 
CCGGTGCACC TCTTGGAGAC GCTGCTGCCG CGGCACCTCC AGATCATCTA CGAGATCAAC 
CAGCGCTTCC TCAACCGGGT GGCGGCCGCA TTCCCAGGGG ACGTAGACCG GCTGCGGCGC 
ATGTCGCTGG TGGAGGAGGG CGCAGTGAAG CGCATCAACA TGGCACACCT GTGCATCGCG 
GGGTCGCACG CCGTCAACGG CGTGGCGCGC ATCCACTCCG AGATCCTCAA GAAGACCATC 
TTCAAAGACT TCTATGAGCT GGAGCCTCAT AAGTTCCAGA ATAAGACCAA CGGCATCACC 
CCTCGGCGCT GGCTGGTTCT GTGTAACCCC GGGCTGGCAG AGGTCATTGC TGAGCGCATC 
GGGGAGGACT TCATCTCTGA CCTGGACCAG CTGCGCAAAC TGCTCTCCTT TGTGGATGAT 
GAAGCTTTCA TTCGGGATGT GGCCAAAGTG AAGCAGGAAA ACAAGTTGAA GTTTGCTGCC 
TACCTAGAGA GGGAATACAA AGTCCACATC AACCCCAACT CACTCTTCGA CATCCAGGTG 
AAGCGGATTC ACGAATATAA ACGACAGCTC CTCAACTGCC TCCATGTCAT CACCCTGTAC 
AACCGCATCA AGAGGGAGCC CAATAAGTTT TTTGTGCCTC GGACTGTGAT GATTGGAGGG 
AAGGCTGCAC CTGGGTACCA CATGGCCAAG ATGATCATCA GACTCGTCAC AGCCATCGGG 
GATGTGGTCA ACCATGACCC GGCAGTGGGT GACCGCCTCC GTGTCATCTT CCTGGAGAAC 
TACCGAGTCT CACTGGCCGA GAAAGTGATC CCAGCTGCAG ACCTCTCTGA GCAGATCTCC 
ACTGCGGGCA CTGAAGCCTC AGGCACCGGC AACATGAAGT TCATGCTCAA CGGGGCTCTG 
ACCATTGGCA CCATGGACGG GGCCAATGTG GAGATGGCAG AAGAGGCGGG AGAGGAAAAC 
TTCTTCATCT TTGGCATGCG GGTGGAGGAT GTGGATAAGC TTGACCAAAG AGGGTACAAT 
GCCCAGGAGT ACTACGATCG CATTCCTGAG CTTCGGCAGG TCATTGAGCA GCTGAGCAGT 
GGCTTCTTCT CCCCCAAACA GCCCGACCTG TTCAAGGACA TTGTCAATAT GCTCATGCAC 
CATGACCGGT TTAAAGTCTT CGCAGATTAT GAAGACTACA TTAAATGCCA GGAGAAAGTC 
AGCGCCTTGT ACAAGAACCC AAGAGAGTGG ACGCGGATGG TGATCCGGAA CATAGCCACC 
TCTGGCAAGT TCTCCAGTGA CCGCACCATT GCCCAGTATG CCCGGGAGAT CTGGGGTGTG 
GAGCCTTCCC GCCAGCGCCT GCCAGCCCCG GATGAGGCCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel variant in the PYGM gene causing late-onset limb-girdle myopathy, ptosis, and camptocormia.
Muscle & nerve57(1)157-160(2018 Jan)
Ch?raud C,Froissart R,Lannes B,Echaniz-Laguna A

The glial phosphorylase of glycogen isoform is reduced in the dorsolateral prefrontal cortex in chronic schizophrenia.
Schizophrenia research177(1-3)37-43(2016 11)
Pinacho R,Vila E,Prades R,Tarrag? T,Castro E,Ferrer I,Ramos B

Taking advantage of an old concept, "illegitimate transcription", for a proposed novel method of genetic diagnosis of McArdle disease.
Genetics in medicine : official journal of the American College of Medical Genetics18(11)1128-1135(2016 Nov)
Garcia-Consuegra I,Bl?zquez A,Rubio JC,Arenas J,Ballester-Lopez A,Gonz?lez-Quintana A,Andreu AL,Pin?s T,Coll-Cant? J,Lucia A,Nogales-Gadea G,Mart?n MA

Genetic evaluation of AMPD1, CPT2, and PGYM metabolic enzymes in patients with chronic fatigue syndrome.
Genetics and molecular research : GMR15(3)(2016 Jul)
Maltese PE,Venturini L,Poplavskaya E,Bertelli M,Cecchin S,Granato M,Nikulina SY,Salmina A,Aksyutina N,Capelli E,Ricevuti G,Lorusso L

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PYGM cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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