RAD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD1
Entrez Gene ID	5810
Full Name	RAD1 checkpoint DNA exonuclease
Synonyms	HRAD1,REC1
General protein information	Preferred Names RAD1 checkpoint DNA exonuclease Names cell cycle checkpoint protein RAD1 DNA repair exonuclease REC1 DNA repair exonuclease rad1 homolog RAD1 checkpoint clamp component RAD1 homolog cell cycle checkpoint protein Hrad1 checkpoint control protein HRAD1 exonuclease homolog RAD1 rad1-like DNA damage checkpoint protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p13.2
Summary	This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009].
Disorder MIM:	603153

mRNA and Protein(s)

mRNA	Protein	Name
NM_002853.3	NP_002844.1	cell cycle checkpoint protein RAD1
NM_002853.4	NP_002844.1	cell cycle checkpoint protein RAD1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD1	NP_002844.1
Gallus gallus (chicken)	RAD1	XP_004937237.1
Magnaporthe oryzae (rice blast fungus)	MGG_17052	XP_003716208.1
Pan troglodytes (chimpanzee)	RAD1	XP_517813.2
Drosophila melanogaster (fruit fly)	Rad1	NP_477440.1
Xenopus tropicalis (tropical clawed frog)	rad1	NP_001016045.1
Canis lupus familiaris (dog)	RAD1	XP_005619459.1
Mus musculus (house mouse)	Rad1	NP_035362.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002255	XP_307928.5
Neurospora crassa	NCU00942	XP_964768.2
	RAD1	XP_001090119.1
Rattus norvegicus (Norway rat)	Rad1	NP_001099889.1
Danio rerio (zebrafish)	rad1	NP_957192.1
Schizosaccharomyces pombe (fission yeast)	rad1	NP_594809.1
Bos taurus (cattle)	RAD1	NP_001179419.1
Caenorhabditis elegans (roundworm)	mrt-2	NP_499521.1

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphisms in cancer susceptibility genes XRCC1, RAD51 and TP53 and the risk of breast cancer in Serbian women.
Krivokuca AM, Cavic MR, Malisic EJ, Rakobradovic JD, Kolarevic-Ivankovic D, Tomasevic ZI, Brankovic-Magic MV
The International journal of biological markers31(3)e258-63(2016 Jul)

Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.
Takeishi Y, Iwaya-Omi R, Ohashi E, Tsurimoto T
The Journal of biological chemistry290(32)19923-32(2015 Aug)

Quantitative proteomic analysis of human substantia nigra in Alzheimer's disease, Huntington's disease and Multiple sclerosis.
Chen S, Lu FF, Seeman P, Liu F
Neurochemical research37(12)2805-13(2012 Dec)

Repair complexes of FEN1 endonuclease, DNA, and Rad9-Hus1-Rad1 are distinguished from their PCNA counterparts by functionally important stability.
Querol-Aud? J, Yan C, Xu X, Tsutakawa SE, Tsai MS, Tainer JA, Cooper PK, Nogales E, Ivanov I
Proceedings of the National Academy of Sciences of the United States of America109(22)8528-33(2012 May)

9-1-1: PCNA's specialized cousin.
Eichinger CS, Jentsch S
Trends in biochemical sciences36(11)563-8(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dominant alleles of RAD51, TP53 and XRCC1 combined genotypes indicated a strong protective role against hereditary breast cancer.
Title: Polymorphisms in cancer susceptibility genes XRCC1, RAD51 and TP53 and the risk of breast cancer in Serbian women.

Intramolecular binding of the rad9 C-terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding.
Title: Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.

Data show models for the ternary PCNA/FEN1/DNA and Rad9-Rad1-Hus1 (9-1-1 complex)/FEN1/DNA assemblies.
Title: Repair complexes of FEN1 endonuclease, DNA, and Rad9-Hus1-Rad1 are distinguished from their PCNA counterparts by functionally important stability.

The RAD1 is loaded to damaged sites where it serves as a platform for the selective recruitment of checkpoint and repair proteins.
Title: 9-1-1: PCNA's specialized cousin.

Observational study of gene-disease association. (HuGE Navigator)
Title: An approach based on a genome-wide association study reveals candidate loci for narcolepsy.

The following RAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03147	NM_002853.4 Latest version!	Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$216.30 $309.00
View Old Accession Versions >>
OHu03147	NM_002853.3	Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$216.30 $309.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD1 ( NM_002853.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002844.1 Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03147
Clone ID Related Accession (Same CDS sequence)	NM_002853.3 , NM_002853.4
Accession Version	NM_002853.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	cell cycle checkpoint protein RAD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037857.1, AF011905.1 and AC026801.7. On Oct 4, 2005 this sequence version replaced NM_002853.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.208746.1, SRR1660809.101247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCTTC TGACCCAACA GATCCAAGAC GAGGATGATC AGTACAGCCT TGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTATC TTGAAAGCTA TTCATTTCCG AGAACATGCC 
ACGTGTTTCG CAACTAAAAA TGGTATCAAA GTAACAGTGG AAAATGCAAA GTGTGTGCAA 
GCAAATGCTT TTATTCAGGC TGGAATATTT CAGGAGTTTA AAGTTCAGGA AGAGTCTGTT 
ACTTTTCGAA TTAATTTAAC TGTCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ATGCCAGGGA CTTTAACTGC ACTTCGAATG TGTTACCAAG GTTATGGTTA CCCTTTGATG 
CTGTTCCTGG AAGAAGGAGG AGTGGTGACA GTCTGCAAAA TCAATACACA GGAACCTGAG 
GAGACCCTGG ACTTTGATTT CTGCAGCACC AATGTTATTA ATAAAATTAT TCTGCAGTCA 
GAGGGGCTCC GTGAAGCATT TTCTGAATTG GATATGACGA GTGAAGTCCT ACAAATTACC 
ATGTCTCCTG ACAAGCCTTA TTTCAGGTTA TCTACTTTTG GAAATGCAGG AAGTTCCCAC 
CTTGACTATC CCAAAGATTC TGATTTGATG GAAGCATTTC ATTGTAATCA GACCCAAGTC 
AACAGATACA AGATTTCCTT ACTGAAACCC TCTACAAAGG CATTAGTCCT ATCTTGTAAG 
GTATCTATTC GGACAGATAA CAGAGGCTTC CTTTCATTAC AGTATATGAT TAGAAATGAA 
GATGGACAAA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAATCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002844.1
CDS	330..1178
Translation	MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

Target ORF information:

RefSeq Version	NM_002853.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002853.3

ORF Insert Sequence:

ATGCCCCTTC TGACCCAACA GATCCAAGAC GAGGATGATC AGTACAGCCT TGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTATC TTGAAAGCTA TTCATTTCCG AGAACATGCC 
ACGTGTTTCG CAACTAAAAA TGGTATCAAA GTAACAGTGG AAAATGCAAA GTGTGTGCAA 
GCAAATGCTT TTATTCAGGC TGGAATATTT CAGGAGTTTA AAGTTCAGGA AGAGTCTGTT 
ACTTTTCGAA TTAATTTAAC TGTCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ATGCCAGGGA CTTTAACTGC ACTTCGAATG TGTTACCAAG GTTATGGTTA CCCTTTGATG 
CTGTTCCTGG AAGAAGGAGG AGTGGTGACA GTCTGCAAAA TCAATACACA GGAACCTGAG 
GAGACCCTGG ACTTTGATTT CTGCAGCACC AATGTTATTA ATAAAATTAT TCTGCAGTCA 
GAGGGGCTCC GTGAAGCATT TTCTGAATTG GATATGACGA GTGAAGTCCT ACAAATTACC 
ATGTCTCCTG ACAAGCCTTA TTTCAGGTTA TCTACTTTTG GAAATGCAGG AAGTTCCCAC 
CTTGACTATC CCAAAGATTC TGATTTGATG GAAGCATTTC ATTGTAATCA GACCCAAGTC 
AACAGATACA AGATTTCCTT ACTGAAACCC TCTACAAAGG CATTAGTCCT ATCTTGTAAG 
GTATCTATTC GGACAGATAA CAGAGGCTTC CTTTCATTAC AGTATATGAT TAGAAATGAA 
GATGGACAAA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAATCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD1 ( NM_002853.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002844.1 Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03147
Clone ID Related Accession (Same CDS sequence)	NM_002853.3 , NM_002853.4
Accession Version	NM_002853.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	cell cycle checkpoint protein RAD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF011905.1 and AC026801.7. On Nov 23, 2018 this sequence version replaced NM_002853.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.208746.1, SRR1660809.101247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000382038.7/ ENSP00000371469.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCCTTC TGACCCAACA GATCCAAGAC GAGGATGATC AGTACAGCCT TGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTATC TTGAAAGCTA TTCATTTCCG AGAACATGCC 
ACGTGTTTCG CAACTAAAAA TGGTATCAAA GTAACAGTGG AAAATGCAAA GTGTGTGCAA 
GCAAATGCTT TTATTCAGGC TGGAATATTT CAGGAGTTTA AAGTTCAGGA AGAGTCTGTT 
ACTTTTCGAA TTAATTTAAC TGTCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ATGCCAGGGA CTTTAACTGC ACTTCGAATG TGTTACCAAG GTTATGGTTA CCCTTTGATG 
CTGTTCCTGG AAGAAGGAGG AGTGGTGACA GTCTGCAAAA TCAATACACA GGAACCTGAG 
GAGACCCTGG ACTTTGATTT CTGCAGCACC AATGTTATTA ATAAAATTAT TCTGCAGTCA 
GAGGGGCTCC GTGAAGCATT TTCTGAATTG GATATGACGA GTGAAGTCCT ACAAATTACC 
ATGTCTCCTG ACAAGCCTTA TTTCAGGTTA TCTACTTTTG GAAATGCAGG AAGTTCCCAC 
CTTGACTATC CCAAAGATTC TGATTTGATG GAAGCATTTC ATTGTAATCA GACCCAAGTC 
AACAGATACA AGATTTCCTT ACTGAAACCC TCTACAAAGG CATTAGTCCT ATCTTGTAAG 
GTATCTATTC GGACAGATAA CAGAGGCTTC CTTTCATTAC AGTATATGAT TAGAAATGAA 
GATGGACAAA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAATCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002844.1
CDS	159..1007
Translation	MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

Target ORF information:

RefSeq Version	NM_002853.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002853.4

ORF Insert Sequence:

ATGCCCCTTC TGACCCAACA GATCCAAGAC GAGGATGATC AGTACAGCCT TGTGGCCAGC 
CTTGACAACG TTAGGAATCT CTCCACTATC TTGAAAGCTA TTCATTTCCG AGAACATGCC 
ACGTGTTTCG CAACTAAAAA TGGTATCAAA GTAACAGTGG AAAATGCAAA GTGTGTGCAA 
GCAAATGCTT TTATTCAGGC TGGAATATTT CAGGAGTTTA AAGTTCAGGA AGAGTCTGTT 
ACTTTTCGAA TTAATTTAAC TGTCCTTTTA GACTGTTTAT CTATTTTTGG ATCAAGTCCT 
ATGCCAGGGA CTTTAACTGC ACTTCGAATG TGTTACCAAG GTTATGGTTA CCCTTTGATG 
CTGTTCCTGG AAGAAGGAGG AGTGGTGACA GTCTGCAAAA TCAATACACA GGAACCTGAG 
GAGACCCTGG ACTTTGATTT CTGCAGCACC AATGTTATTA ATAAAATTAT TCTGCAGTCA 
GAGGGGCTCC GTGAAGCATT TTCTGAATTG GATATGACGA GTGAAGTCCT ACAAATTACC 
ATGTCTCCTG ACAAGCCTTA TTTCAGGTTA TCTACTTTTG GAAATGCAGG AAGTTCCCAC 
CTTGACTATC CCAAAGATTC TGATTTGATG GAAGCATTTC ATTGTAATCA GACCCAAGTC 
AACAGATACA AGATTTCCTT ACTGAAACCC TCTACAAAGG CATTAGTCCT ATCTTGTAAG 
GTATCTATTC GGACAGATAA CAGAGGCTTC CTTTCATTAC AGTATATGAT TAGAAATGAA 
GATGGACAAA TATGTTTTGT GGAATATTAC TGCTGCCCTG ATGAAGAAGT TCCTGAATCT 
GAGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphisms in cancer susceptibility genes XRCC1, RAD51 and TP53 and the risk of breast cancer in Serbian women.
The International journal of biological markers31(3)e258-63(2016 Jul)
Krivokuca AM,Cavic MR,Malisic EJ,Rakobradovic JD,Kolarevic-Ivankovic D,Tomasevic ZI,Brankovic-Magic MV

Intramolecular Binding of the Rad9 C Terminus in the Checkpoint Clamp Rad9-Hus1-Rad1 Is Closely Linked with Its DNA Binding.
The Journal of biological chemistry290(32)19923-32(2015 Aug)
Takeishi Y,Iwaya-Omi R,Ohashi E,Tsurimoto T

Quantitative proteomic analysis of human substantia nigra in Alzheimer's disease, Huntington's disease and Multiple sclerosis.
Neurochemical research37(12)2805-13(2012 Dec)
Chen S,Lu FF,Seeman P,Liu F

Repair complexes of FEN1 endonuclease, DNA, and Rad9-Hus1-Rad1 are distinguished from their PCNA counterparts by functionally important stability.
Proceedings of the National Academy of Sciences of the United States of America109(22)8528-33(2012 May)
Querol-Aud? J,Yan C,Xu X,Tsutakawa SE,Tsai MS,Tainer JA,Cooper PK,Nogales E,Ivanov I

9-1-1: PCNA's specialized cousin.
Trends in biochemical sciences36(11)563-8(2011 Nov)
Eichinger CS,Jentsch S

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RAD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報