ACTA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTA1
Entrez Gene ID	58
Full Name	actin, alpha 1, skeletal muscle
Synonyms	ACTA,ASMA,CFTD,CFTD1,CFTDM,MPFD,NEM1,NEM2,NEM3,SHPM
General protein information	Preferred Names actin, alpha 1, skeletal muscle Names actin, alpha skeletal muscle nemaline myopathy type 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.13
Summary	The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008].
Disorder MIM:	102610
Disorder Html:	Nemaline myopathy 3, autosomal dominant or recessive, 161800 (3); Myopathy, actin, congenital, with excess of thin myofilaments, 161800 (3); Myopathy, actin, congenital, with cores, 161800 (3); Myopathy, congenital, with fiber-type disproportion 1, 255310 (3); ?Myopathy, scapulohumeroperoneal, 616852 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100.4	NP_001091.1	actin, alpha skeletal muscle
NM_001100.3	NP_001091.1	actin, alpha skeletal muscle

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTA1	XP_005618896.1
Rattus norvegicus (Norway rat)	Acta1	NP_062085.1
Danio rerio (zebrafish)	acta1b	NP_999949.2
Mus musculus (house mouse)	Acta1	NP_033736.1
Pan troglodytes (chimpanzee)	ACTA1	XP_003308856.1
Bos taurus (cattle)	ACTA1	NP_776650.1
Arabidopsis thaliana (thale cress)	AT2G42100	NP_181740.1
Homo sapiens (human)	ACTA1	NP_001091.1
	ACTA1	XP_002802172.1
Gallus gallus (chicken)	ACTA1	NP_001026234.1
Xenopus tropicalis (tropical clawed frog)	act3	NP_989076.1

Gene Ontology
Bibliography

Related articles in PubMed

Clinical and Histologic?Findings in ACTA1-Related Nemaline Myopathy: Case Series and Review of the Literature.
Moreno CAM, Abath Neto O, Donkervoort S, Hu Y, Reed UC, Oliveira ASB, B?nnemann C, Zanoteli E
Pediatric neurology7511-16(2017 Oct)

Defective thymic output in WAS patients is associated with abnormal actin organization.
Li W, Sun X, Wang J, Zhao Q, Dai R, Wang Y, Zhou L, Westerberg L, Ding Y, Zhao X, Liu C
Scientific reports7(1)11978(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Over-expression of TNC, SMA, and vimentin were significantly correlated with the lower overall survival in prostate cancer patients.
Title: Tenascin-C is a potential cancer-associated fibroblasts marker and predicts poor prognosis in prostate cancer.

ANA and ASMA evaluation in patients with liver transplantation and no history of autoimmune disease has no clinical relevance, since it varies in time and is not related to any risk factors or liver injury. Routine autoimmunity evaluation should be avoided.
Title: Lack of Clinical Relevance of ANA and ASMA Positivity in Patients with Liver Transplantation without a History of Autoimmune Diseases.

Shorter than normal thin filament length contributes to the impaired force generation in patients with thin filament myopathy, but only in those who harbor specific mutations in NEB or ACTA1.
Title: Mutation-specific effects on thin filament length in thin filament myopathy.

Upon actin engagement, the N-terminal "strap" and helix 1 are displaced from the vinculin tail helical bundle to mediate actin bundling.
Title: The Structural Basis of Actin Organization by Vinculin and Metavinculin.

The authors propose that Lpd delivers Ena/VASP proteins to growing barbed ends and increases their actin polymerase activity by tethering them to actin filaments.
Title: Lamellipodin promotes actin assembly by clustering Ena/VASP proteins and tethering them to actin filaments.

The following ACTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18695	NM_001100.4 Latest version!	Homo sapiens actin alpha 1, skeletal muscle (ACTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu18695	NM_001100.3	Homo sapiens actin alpha 1, skeletal muscle (ACTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTA1 ( NM_001100.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091.1 Homo sapiens actin alpha 1, skeletal muscle (ACTA1), mRNA.

Price & Availability↑

CloneID	OHu18695
Clone ID Related Accession (Same CDS sequence)	NM_001100.4 , NM_001100.3
Accession Version	NM_001100.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	actin, alpha skeletal muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL598491.1, BC012597.1 and BX648545.1. On Aug 31, 2019 this sequence version replaced NM_001100.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012597.1, J00068.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366684.7/ ENSP00000355645.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGCGACG AAGACGAGAC CACCGCCCTC GTGTGCGACA ATGGCTCCGG CCTGGTGAAA 
GCCGGCTTCG CCGGGGATGA CGCCCCTAGG GCCGTGTTCC CGTCCATCGT GGGCCGCCCC 
CGACACCAGG GCGTCATGGT CGGTATGGGT CAGAAAGATT CCTACGTGGG CGACGAGGCT 
CAGAGCAAGA GAGGTATCCT GACCCTGAAG TACCCTATCG AGCACGGCAT CATCACCAAC 
TGGGATGACA TGGAGAAGAT CTGGCACCAC ACCTTCTACA ACGAGCTTCG CGTGGCTCCC 
GAGGAGCACC CCACCCTGCT CACCGAGGCC CCCCTCAATC CCAAGGCCAA CCGCGAGAAG 
ATGACCCAGA TCATGTTTGA GACCTTCAAC GTGCCCGCCA TGTACGTGGC CATCCAGGCC 
GTGCTGTCCC TCTACGCCTC CGGCAGGACC ACCGGCATCG TGCTGGACTC CGGCGACGGC 
GTCACCCACA ACGTGCCCAT TTATGAGGGC TACGCGCTGC CGCACGCCAT CATGCGCCTG 
GACCTGGCGG GCCGCGATCT CACCGACTAC CTGATGAAGA TCCTCACTGA GCGTGGCTAC 
TCCTTCGTGA CCACAGCTGA GCGCGAGATC GTGCGCGACA TCAAGGAGAA GCTGTGCTAC 
GTGGCCCTGG ACTTCGAGAA CGAGATGGCG ACGGCCGCCT CCTCCTCCTC CCTGGAAAAG 
AGCTACGAGC TGCCAGACGG GCAGGTCATC ACCATCGGCA ACGAGCGCTT CCGCTGCCCG 
GAGACGCTCT TCCAGCCCTC CTTCATCGGT ATGGAGTCGG CGGGCATTCA CGAGACCACC 
TACAACAGCA TCATGAAGTG TGACATCGAC ATCAGGAAGG ACCTGTATGC CAACAACGTC 
ATGTCGGGGG GCACCACGAT GTACCCTGGG ATCGCTGACC GCATGCAGAA AGAGATCACC 
GCGCTGGCAC CCAGCACCAT GAAGATCAAG ATCATCGCCC CGCCGGAGCG CAAATACTCG 
GTGTGGATCG GCGGCTCCAT CCTGGCCTCG CTGTCCACCT TCCAGCAGAT GTGGATCACC 
AAGCAGGAGT ACGACGAGGC CGGCCCTTCC ATCGTCCACC GCAAATGCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091.1
CDS	104..1237
Translation	MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001100.4
Organism	Homo sapiens(human)
Definition	Homo sapiens actin alpha 1, skeletal muscle (ACTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100.4

ORF Insert Sequence:

ATGTGCGACG AAGACGAGAC CACCGCCCTC GTGTGCGACA ATGGCTCCGG CCTGGTGAAA 
GCCGGCTTCG CCGGGGATGA CGCCCCTAGG GCCGTGTTCC CGTCCATCGT GGGCCGCCCC 
CGACACCAGG GCGTCATGGT CGGTATGGGT CAGAAAGATT CCTACGTGGG CGACGAGGCT 
CAGAGCAAGA GAGGTATCCT GACCCTGAAG TACCCTATCG AGCACGGCAT CATCACCAAC 
TGGGATGACA TGGAGAAGAT CTGGCACCAC ACCTTCTACA ACGAGCTTCG CGTGGCTCCC 
GAGGAGCACC CCACCCTGCT CACCGAGGCC CCCCTCAATC CCAAGGCCAA CCGCGAGAAG 
ATGACCCAGA TCATGTTTGA GACCTTCAAC GTGCCCGCCA TGTACGTGGC CATCCAGGCC 
GTGCTGTCCC TCTACGCCTC CGGCAGGACC ACCGGCATCG TGCTGGACTC CGGCGACGGC 
GTCACCCACA ACGTGCCCAT TTATGAGGGC TACGCGCTGC CGCACGCCAT CATGCGCCTG 
GACCTGGCGG GCCGCGATCT CACCGACTAC CTGATGAAGA TCCTCACTGA GCGTGGCTAC 
TCCTTCGTGA CCACAGCTGA GCGCGAGATC GTGCGCGACA TCAAGGAGAA GCTGTGCTAC 
GTGGCCCTGG ACTTCGAGAA CGAGATGGCG ACGGCCGCCT CCTCCTCCTC CCTGGAAAAG 
AGCTACGAGC TGCCAGACGG GCAGGTCATC ACCATCGGCA ACGAGCGCTT CCGCTGCCCG 
GAGACGCTCT TCCAGCCCTC CTTCATCGGT ATGGAGTCGG CGGGCATTCA CGAGACCACC 
TACAACAGCA TCATGAAGTG TGACATCGAC ATCAGGAAGG ACCTGTATGC CAACAACGTC 
ATGTCGGGGG GCACCACGAT GTACCCTGGG ATCGCTGACC GCATGCAGAA AGAGATCACC 
GCGCTGGCAC CCAGCACCAT GAAGATCAAG ATCATCGCCC CGCCGGAGCG CAAATACTCG 
GTGTGGATCG GCGGCTCCAT CCTGGCCTCG CTGTCCACCT TCCAGCAGAT GTGGATCACC 
AAGCAGGAGT ACGACGAGGC CGGCCCTTCC ATCGTCCACC GCAAATGCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTA1 ( NM_001100.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091.1 Homo sapiens actin alpha 1, skeletal muscle (ACTA1), mRNA.

Price & Availability↑

CloneID	OHu18695
Clone ID Related Accession (Same CDS sequence)	NM_001100.4 , NM_001100.3
Accession Version	NM_001100.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	actin, alpha skeletal muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL598491.1, BC012597.1 and BX648545.1. This sequence is a reference standard in the RefSeqGene project. On May 7, 2004 this sequence version replaced NM_001100.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279876.1889.1, BC012597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGCGACG AAGACGAGAC CACCGCCCTC GTGTGCGACA ATGGCTCCGG CCTGGTGAAA 
GCCGGCTTCG CCGGGGATGA CGCCCCTAGG GCCGTGTTCC CGTCCATCGT GGGCCGCCCC 
CGACACCAGG GCGTCATGGT CGGTATGGGT CAGAAAGATT CCTACGTGGG CGACGAGGCT 
CAGAGCAAGA GAGGTATCCT GACCCTGAAG TACCCTATCG AGCACGGCAT CATCACCAAC 
TGGGATGACA TGGAGAAGAT CTGGCACCAC ACCTTCTACA ACGAGCTTCG CGTGGCTCCC 
GAGGAGCACC CCACCCTGCT CACCGAGGCC CCCCTCAATC CCAAGGCCAA CCGCGAGAAG 
ATGACCCAGA TCATGTTTGA GACCTTCAAC GTGCCCGCCA TGTACGTGGC CATCCAGGCC 
GTGCTGTCCC TCTACGCCTC CGGCAGGACC ACCGGCATCG TGCTGGACTC CGGCGACGGC 
GTCACCCACA ACGTGCCCAT TTATGAGGGC TACGCGCTGC CGCACGCCAT CATGCGCCTG 
GACCTGGCGG GCCGCGATCT CACCGACTAC CTGATGAAGA TCCTCACTGA GCGTGGCTAC 
TCCTTCGTGA CCACAGCTGA GCGCGAGATC GTGCGCGACA TCAAGGAGAA GCTGTGCTAC 
GTGGCCCTGG ACTTCGAGAA CGAGATGGCG ACGGCCGCCT CCTCCTCCTC CCTGGAAAAG 
AGCTACGAGC TGCCAGACGG GCAGGTCATC ACCATCGGCA ACGAGCGCTT CCGCTGCCCG 
GAGACGCTCT TCCAGCCCTC CTTCATCGGT ATGGAGTCGG CGGGCATTCA CGAGACCACC 
TACAACAGCA TCATGAAGTG TGACATCGAC ATCAGGAAGG ACCTGTATGC CAACAACGTC 
ATGTCGGGGG GCACCACGAT GTACCCTGGG ATCGCTGACC GCATGCAGAA AGAGATCACC 
GCGCTGGCAC CCAGCACCAT GAAGATCAAG ATCATCGCCC CGCCGGAGCG CAAATACTCG 
GTGTGGATCG GCGGCTCCAT CCTGGCCTCG CTGTCCACCT TCCAGCAGAT GTGGATCACC 
AAGCAGGAGT ACGACGAGGC CGGCCCTTCC ATCGTCCACC GCAAATGCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091.1
CDS	106..1239
Translation	MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001100.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actin alpha 1, skeletal muscle (ACTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100.3

ORF Insert Sequence:

ATGTGCGACG AAGACGAGAC CACCGCCCTC GTGTGCGACA ATGGCTCCGG CCTGGTGAAA 
GCCGGCTTCG CCGGGGATGA CGCCCCTAGG GCCGTGTTCC CGTCCATCGT GGGCCGCCCC 
CGACACCAGG GCGTCATGGT CGGTATGGGT CAGAAAGATT CCTACGTGGG CGACGAGGCT 
CAGAGCAAGA GAGGTATCCT GACCCTGAAG TACCCTATCG AGCACGGCAT CATCACCAAC 
TGGGATGACA TGGAGAAGAT CTGGCACCAC ACCTTCTACA ACGAGCTTCG CGTGGCTCCC 
GAGGAGCACC CCACCCTGCT CACCGAGGCC CCCCTCAATC CCAAGGCCAA CCGCGAGAAG 
ATGACCCAGA TCATGTTTGA GACCTTCAAC GTGCCCGCCA TGTACGTGGC CATCCAGGCC 
GTGCTGTCCC TCTACGCCTC CGGCAGGACC ACCGGCATCG TGCTGGACTC CGGCGACGGC 
GTCACCCACA ACGTGCCCAT TTATGAGGGC TACGCGCTGC CGCACGCCAT CATGCGCCTG 
GACCTGGCGG GCCGCGATCT CACCGACTAC CTGATGAAGA TCCTCACTGA GCGTGGCTAC 
TCCTTCGTGA CCACAGCTGA GCGCGAGATC GTGCGCGACA TCAAGGAGAA GCTGTGCTAC 
GTGGCCCTGG ACTTCGAGAA CGAGATGGCG ACGGCCGCCT CCTCCTCCTC CCTGGAAAAG 
AGCTACGAGC TGCCAGACGG GCAGGTCATC ACCATCGGCA ACGAGCGCTT CCGCTGCCCG 
GAGACGCTCT TCCAGCCCTC CTTCATCGGT ATGGAGTCGG CGGGCATTCA CGAGACCACC 
TACAACAGCA TCATGAAGTG TGACATCGAC ATCAGGAAGG ACCTGTATGC CAACAACGTC 
ATGTCGGGGG GCACCACGAT GTACCCTGGG ATCGCTGACC GCATGCAGAA AGAGATCACC 
GCGCTGGCAC CCAGCACCAT GAAGATCAAG ATCATCGCCC CGCCGGAGCG CAAATACTCG 
GTGTGGATCG GCGGCTCCAT CCTGGCCTCG CTGTCCACCT TCCAGCAGAT GTGGATCACC 
AAGCAGGAGT ACGACGAGGC CGGCCCTTCC ATCGTCCACC GCAAATGCTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical and Histologic?Findings in ACTA1-Related Nemaline Myopathy: Case Series and Review of the Literature.
Pediatric neurology7511-16(2017 Oct)
Moreno CAM,Abath Neto O,Donkervoort S,Hu Y,Reed UC,Oliveira ASB,B?nnemann C,Zanoteli E

Defective thymic output in WAS patients is associated with abnormal actin organization.
Scientific reports7(1)11978(2017 Sep)
Li W,Sun X,Wang J,Zhao Q,Dai R,Wang Y,Zhou L,Westerberg L,Ding Y,Zhao X,Liu C

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ACTA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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