ZP4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZP4
Entrez Gene ID	57829
Full Name	zona pellucida glycoprotein 4
Synonyms	ZBP,ZP1,ZPB,Zp-4
General protein information	Preferred Names zona pellucida glycoprotein 4 Names zona pellucida sperm-binding protein 4 zona pellucida protein B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q43
Summary	The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008].
Disorder MIM:	613514

mRNA and Protein(s)

mRNA	Protein	Name
NM_021186.4	NP_067009.1	zona pellucida sperm-binding protein 4 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZP4	NP_067009.1
Canis lupus familiaris (dog)	ZP4	XP_536329.3
Gallus gallus (chicken)	ZP4	NP_990210.1
Pan troglodytes (chimpanzee)	ZP4	XP_525105.2
	ZP4	XP_001096846.1
Rattus norvegicus (Norway rat)	Zp4	NP_758833.2
Xenopus tropicalis (tropical clawed frog)	zp4	NP_988854.1
Bos taurus (cattle)	ZP4	NP_776400.1

Gene Ontology
Bibliography

Related articles in PubMed

A common 'aggregation-prone' interface possibly participates in the self-assembly of human zona pellucida proteins.
Louros NN, Chrysina ED, Baltatzis GE, Patsouris ES, Hamodrakas SJ, Iconomidou VA
FEBS letters590(5)619-30(2016 Mar)

Human sperm degradation of zona pellucida proteins contributes to fertilization.
Sald?var-Hern?ndez A, Gonz?lez-Gonz?lez ME, S?nchez-Tusi? A, Maldonado-Rosas I, L?pez P, Trevi?o CL, Larrea F, Chirinos M
Reproductive biology and endocrinology : RB&E1399(2015 Sep)

Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome.
Meczekalski B, Nawrot R, Nowak W, Czyzyk A, Kedzia H, Gozdzicka-Jozefiak A
Journal of endocrinological investigation38(7)791-7(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ZO4 is highly expressed in ovarian tissue in PCOS patients with regular cycle and without hyperandrogenism; in some patients with PCOS and infertility, ZP4 mutation can be found.
Title: Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome.

Human ZP4 is not sufficient to support human sperm binding to the zona pellucida in transgenic mice; other zona proteins may be needed for human gamete recognition.
Title: Human ZP4 is not sufficient for taxon-specific sperm recognition of the zona pellucida in transgenic mice.

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The following ZP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu12154	NM_021186.4 Latest version!	Homo sapiens zona pellucida glycoprotein 4 (ZP4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZP4 ( NM_021186.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_067009.1 Homo sapiens zona pellucida glycoprotein 4 (ZP4), mRNA.

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CloneID	OHu12154
Clone ID Related Accession (Same CDS sequence)	NM_021186.4
Accession Version	NM_021186.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	zona pellucida sperm-binding protein 4 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314151.1 and DV080723.1. This sequence is a reference standard in the RefSeqGene project. On May 12, 2016 this sequence version replaced NM_021186.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314151.1, BC069521.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTGC TGCGGTGCGT TTTGCTGTGT GTTTCATTAT CTCTTGCTGT GAGTGGCCAG 
CATAAGCCTG AGGCACCAGA TTATTCCAGT GTGCTCCACT GTGGGCCGTG GAGCTTCCAG 
TTTGCTGTAA ACCTCAACCA GGAGGCAACG TCTCCTCCTG TACTAATAGC TTGGGACAAC 
CAAGGGCTGC TGCACGAGCT GCAGAATGAC TCCGACTGTG GCACCTGGAT AAGAAAAGGT 
CCAGGCAGCT CCGTGGTGTT GGAGGCAACC TATAGCAGCT GCTATGTCAC TGAGTGGGAC 
TCCCACTACA TCATGCCAGT TGGAGTTGAA GGAGCAGGCG CGGCTGAACA CAAGGTGGTT 
ACAGAGAGGA AGCTGCTCAA GTGTCCTATG GATCTTCTAG CCCGAGATGC TCCAGATACT 
GACTGGTGTG ACTCCATCCC AGCACGGGAC AGACTGCCAT GTGCACCTTC ACCCATCTCT 
CGAGGAGACT GTGAAGGGCT AGGCTGTTGT TATAGCTCTG AAGAGGTGAA TTCCTGCTAC 
TATGGAAACA CTGTGACCTT GCATTGTACC CGAGAGGGCC ATTTCTCTAT TGCTGTGTCT 
CGGAACGTGA CCTCGCCACC ACTGCTCTTG GATTCTGTGC GCTTGGCCCT TAGGAATGAC 
AGTGCGTGTA ACCCTGTGAT GGCAACACAA GCTTTTGTTC TGTTCCAGTT TCCATTTACT 
TCCTGTGGCA CCACAAGACA GATCACTGGA GACCGAGCAG TATATGAAAA TGAACTGGTG 
GCAACTAGGG ATGTGAAAAA TGGGAGCCGT GGCTCTGTCA CTCGTGACAG CATCTTCAGG 
CTCCATGTCA GCTGCAGCTA CTCAGTAAGT AGCAACTCTC TCCCAATCAA TGTCCAGGTT 
TTCACTCTCC CACCACCCTT TCCTGAGACC CAGCCTGGAC CCCTCACTCT GGAACTTCAG 
ATTGCCAAAG ATAAAAACTA TGGCTCTTAC TACGGTGTTG GTGACTACCC AGTGGTGAAG 
TTGCTTCGGG ATCCCATTTA CGTGGAGGTC TCCATCCTTC ACAGAACAGA CCCCTACCTG 
GGGCTGCTCC TACAACAGTG TTGGGCAACA CCCAGCACTG ACCCCCTGAG TCAGCCACAG 
TGGCCCATCC TGGTAAAGGG CTGCCCCTAC ATTGGAGACA ACTATCAGAC CCAGCTGATC 
CCTGTCCAGA AAGCCTTGGA TCTTCCATTT CCCTCTCACC ACCAGCGCTT CAGCATCTTC 
ACCTTCAGCT TTGTGAACCC TACAGTGGAG AAACAGGCCC TCAGGGGACC GGTGCATCTG 
CACTGCAGCG TGTCAGTCTG CCAGCCTGCT GAGACACCAT CCTGTGTGGT GACCTGTCCT 
GATCTCAGTC GAAGAAGAAA TTTTGACAAC AGTTCTCAGA ACACTACTGC TAGTGTTTCT 
AGCAAAGGCC CCATGATTCT ACTCCAAGCC ACTAAGGACC CTCCAGAAAA GCTCCGTGTT 
CCTGTAGACT CGAAAGTTCT GTGGGTGGCA GGCCTTTCTG GGACCTTAAT CCTTGGAGCC 
TTGTTAGTAT CCTACTTGGC TGTCAAGAAA CAGAAGAGTT GCCCAGACCA AATGTGTCAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067009.1
CDS	288..1910
Translation	MWLLRCVLLCVSLSLAVSGQHKPEAPDYSSVLHCGPWSFQFAVNLNQEATSPPVLIAWDNQGLLHELQNDSDCGTWIRKGPGSSVVLEATYSSCYVTEWDSHYIMPVGVEGAGAAEHKVVTERKLLKCPMDLLARDAPDTDWCDSIPARDRLPCAPSPISRGDCEGLGCCYSSEEVNSCYYGNTVTLHCTREGHFSIAVSRNVTSPPLLLDSVRLALRNDSACNPVMATQAFVLFQFPFTSCGTTRQITGDRAVYENELVATRDVKNGSRGSVTRDSIFRLHVSCSYSVSSNSLPINVQVFTLPPPFPETQPGPLTLELQIAKDKNYGSYYGVGDYPVVKLLRDPIYVEVSILHRTDPYLGLLLQQCWATPSTDPLSQPQWPILVKGCPYIGDNYQTQLIPVQKALDLPFPSHHQRFSIFTFSFVNPTVEKQALRGPVHLHCSVSVCQPAETPSCVVTCPDLSRRRNFDNSSQNTTASVSSKGPMILLQATKDPPEKLRVPVDSKVLWVAGLSGTLILGALLVSYLAVKKQKSCPDQMCQ

Target ORF information:

RefSeq Version	NM_021186.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zona pellucida glycoprotein 4 (ZP4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021186.4

ORF Insert Sequence:

ATGTGGCTGC TGCGGTGCGT TTTGCTGTGT GTTTCATTAT CTCTTGCTGT GAGTGGCCAG 
CATAAGCCTG AGGCACCAGA TTATTCCAGT GTGCTCCACT GTGGGCCGTG GAGCTTCCAG 
TTTGCTGTAA ACCTCAACCA GGAGGCAACG TCTCCTCCTG TACTAATAGC TTGGGACAAC 
CAAGGGCTGC TGCACGAGCT GCAGAATGAC TCCGACTGTG GCACCTGGAT AAGAAAAGGT 
CCAGGCAGCT CCGTGGTGTT GGAGGCAACC TATAGCAGCT GCTATGTCAC TGAGTGGGAC 
TCCCACTACA TCATGCCAGT TGGAGTTGAA GGAGCAGGCG CGGCTGAACA CAAGGTGGTT 
ACAGAGAGGA AGCTGCTCAA GTGTCCTATG GATCTTCTAG CCCGAGATGC TCCAGATACT 
GACTGGTGTG ACTCCATCCC AGCACGGGAC AGACTGCCAT GTGCACCTTC ACCCATCTCT 
CGAGGAGACT GTGAAGGGCT AGGCTGTTGT TATAGCTCTG AAGAGGTGAA TTCCTGCTAC 
TATGGAAACA CTGTGACCTT GCATTGTACC CGAGAGGGCC ATTTCTCTAT TGCTGTGTCT 
CGGAACGTGA CCTCGCCACC ACTGCTCTTG GATTCTGTGC GCTTGGCCCT TAGGAATGAC 
AGTGCGTGTA ACCCTGTGAT GGCAACACAA GCTTTTGTTC TGTTCCAGTT TCCATTTACT 
TCCTGTGGCA CCACAAGACA GATCACTGGA GACCGAGCAG TATATGAAAA TGAACTGGTG 
GCAACTAGGG ATGTGAAAAA TGGGAGCCGT GGCTCTGTCA CTCGTGACAG CATCTTCAGG 
CTCCATGTCA GCTGCAGCTA CTCAGTAAGT AGCAACTCTC TCCCAATCAA TGTCCAGGTT 
TTCACTCTCC CACCACCCTT TCCTGAGACC CAGCCTGGAC CCCTCACTCT GGAACTTCAG 
ATTGCCAAAG ATAAAAACTA TGGCTCTTAC TACGGTGTTG GTGACTACCC AGTGGTGAAG 
TTGCTTCGGG ATCCCATTTA CGTGGAGGTC TCCATCCTTC ACAGAACAGA CCCCTACCTG 
GGGCTGCTCC TACAACAGTG TTGGGCAACA CCCAGCACTG ACCCCCTGAG TCAGCCACAG 
TGGCCCATCC TGGTAAAGGG CTGCCCCTAC ATTGGAGACA ACTATCAGAC CCAGCTGATC 
CCTGTCCAGA AAGCCTTGGA TCTTCCATTT CCCTCTCACC ACCAGCGCTT CAGCATCTTC 
ACCTTCAGCT TTGTGAACCC TACAGTGGAG AAACAGGCCC TCAGGGGACC GGTGCATCTG 
CACTGCAGCG TGTCAGTCTG CCAGCCTGCT GAGACACCAT CCTGTGTGGT GACCTGTCCT 
GATCTCAGTC GAAGAAGAAA TTTTGACAAC AGTTCTCAGA ACACTACTGC TAGTGTTTCT 
AGCAAAGGCC CCATGATTCT ACTCCAAGCC ACTAAGGACC CTCCAGAAAA GCTCCGTGTT 
CCTGTAGACT CGAAAGTTCT GTGGGTGGCA GGCCTTTCTG GGACCTTAAT CCTTGGAGCC 
TTGTTAGTAT CCTACTTGGC TGTCAAGAAA CAGAAGAGTT GCCCAGACCA AATGTGTCAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A common 'aggregation-prone' interface possibly participates in the self-assembly of human zona pellucida proteins.
FEBS letters590(5)619-30(2016 Mar)
Louros NN,Chrysina ED,Baltatzis GE,Patsouris ES,Hamodrakas SJ,Iconomidou VA

Human sperm degradation of zona pellucida proteins contributes to fertilization.
Reproductive biology and endocrinology : RB&E1399(2015 Sep)
Sald?var-Hern?ndez A,Gonz?lez-Gonz?lez ME,S?nchez-Tusi? A,Maldonado-Rosas I,L?pez P,Trevi?o CL,Larrea F,Chirinos M

Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome.
Journal of endocrinological investigation38(7)791-7(2015 Jul)
Meczekalski B,Nawrot R,Nowak W,Czyzyk A,Kedzia H,Gozdzicka-Jozefiak A

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ZP4 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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