RBAK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RBAK
Entrez Gene ID	57786
Full Name	RB associated KRAB zinc finger
Synonyms	ZNF769
General protein information	Preferred Names RB associated KRAB zinc finger Names RB-associated KRAB zinc finger protein RB-associated KRAB repressor zinc finger protein 769
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.1
Summary	This gene encodes a nuclear protein which interacts with the tumor suppressor retinoblastoma 1. The two interacting proteins are thought to act as a transcriptional repressor for promoters which are activated by the E2F1 transcription factor. This protein contains a Kruppel-associated box (KRAB), which is a transcriptional repressor motif. Read-through transcripts that include exons from the downstream gene LOC389458 are expressed from this locus. [provided by RefSeq, Mar 2011].
Disorder MIM:	608191

mRNA and Protein(s)

mRNA	Protein	Name
NM_001204456.2	NP_001191385.1	RB-associated KRAB zinc finger protein
NM_021163.3	NP_066986.1	RB-associated KRAB zinc finger protein
NM_001204456.1	NP_001191385.1	RB-associated KRAB zinc finger protein
NM_021163.4	NP_066986.1	RB-associated KRAB zinc finger protein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rbak	NP_001178593.1
Homo sapiens (human)	RBAK	NP_066986.1
Bos taurus (cattle)	RBAK	NP_001178185.1
Mus musculus (house mouse)	Rbak	NP_001038947.1
Canis lupus familiaris (dog)	RBAK	XP_005621185.1
Pan troglodytes (chimpanzee)	RBAK	XP_001139205.1

Gene Ontology
Bibliography

Related articles in PubMed

[Review for the regulatory functions of KRAB zinc finger proteins in embryonic development and tumorgenesis of higher vertebrates].
Ma ZF, Yang D, He FC, Jiang Y
Yi chuan = Hereditas32(5)431-6(2010 May)

Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
Trynka G, Zhernakova A, Romanos J, Franke L, Hunt KA, Turner G, Bruinenberg M, Heap GA, Platteel M, Ryan AW, de Kovel C, Holmes GK, Howdle PD, Walters JR, Sanders DS, Mulder CJ, Mearin ML, Verbeek WH, Trimble V, Stevens FM, Kelleher D, Barisani D, Bardella MT, McManus R, van Heel DA, Wijmenga C
Gut58(8)1078-83(2009 Aug)

A census of human transcription factors: function, expression and evolution.
Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM
Nature reviews. Genetics10(4)252-63(2009 04)

Identification of candidate genes for human pituitary development by EST analysis.
Ma Y, Qi X, Du J, Song S, Feng D, Qi J, Zhu Z, Zhang X, Xiao H, Han Z, Hao X
BMC genomics10109(2009 Mar)

Examination of chromosome 7p22 candidate genes RBaK, PMS2 and GNA12 in familial hyperaldosteronism type II.
Jeske YW, So A, Kelemen L, Sukor N, Willys C, Bulmer B, Gordon RD, Duffy D, Stowasser M
Clinical and experimental pharmacology & physiology35(4)380-5(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that data on the regulatory functions of the KRAB-ZFP in embryonic development and tumorgenesis will benefit the understanding of biological roles of KRAB-ZFP in different physiological and pathological states.
Title: [Review for the regulatory functions of KRAB zinc finger proteins in embryonic development and tumorgenesis of higher vertebrates].

Observational study of gene-disease association. (HuGE Navigator)
Title: Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.

The role of RBaK, PMS2, and GNA12 in the inheritance of familial hyperaldosteronism type II were studied.
Title: Examination of chromosome 7p22 candidate genes RBaK, PMS2 and GNA12 in familial hyperaldosteronism type II.

study found no evidence of mutations in the coding sequence and splice sites of RBaK in association with familial hyperaldosteronism type II
Title: No evidence for coding region mutations in the retinoblastoma-associated Kruppel-associated box protein gene (RBaK) causing familial hyperaldosteronism type II.

RBaK interacts with the androgen receptor and increases its transcriptional activity.
Title: The retinoblastoma protein-associated transcription repressor RBaK interacts with the androgen receptor and enhances its transcriptional activity.

The following RBAK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RBAK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10623	NM_021163.4 Latest version!	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu10623	NM_001204456.2 Latest version!	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
View Old Accession Versions >>
OHu10623	NM_001204456.1	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu10623	NM_021163.3	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RBAK ( NM_001204456.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191385.1 Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10623
Clone ID Related Accession (Same CDS sequence)	NM_001204456.2 , NM_021163.3 , NM_001204456.1 , NM_021163.4
Accession Version	NM_001204456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	RB-associated KRAB zinc finger protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293069.1 and AC092032.5. On May 31, 2019 this sequence version replaced NM_001204456.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK293069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191385.1
CDS	286..2430
Translation	MNTLQGPVSFKDVAVDFTQEEWQQLDPDEKITYRDVMLENYSHLVSVGYDTTKPNVIIKLEQGEEPWIMGGEFPCQHSPEAWRVDDLIERIQENEDKHSRQAACINSKTLTEEKENTFSQIYMETSLVPSSIIAHNCVSCGKNLESISQLISSDGSYARTKPDECNECGKTYHGEKMCEFNQNGDTYSHNEENILQKISILEKPFEYNECMEALDNEAVFIAHKRAYIGEKPYEWNDSGPDFIQMSNFNAYQRSQMEMKPFECSECGKSFCKKSKFIIHQRAHTGEKPYECNVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHLRTHSGEKPYECSECGKTFCQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALSDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTHTGEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECNECGKTFNLNSAFIRHRKVHTEEKSHECSECGKFSQLYLTDHHTAHLEEKPYECNECGKTFLVNSAFDGHQPLPKGEKSYECNVCGKLFNELSYYTEHYRSHSEEKPYGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFSRMSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHRRGNMNVLDVENL

Target ORF information:

RefSeq Version	NM_001204456.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204456.2

ORF Insert Sequence:

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBAK ( NM_021163.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066986.1 Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10623
Clone ID Related Accession (Same CDS sequence)	NM_001204456.2 , NM_021163.3 , NM_001204456.1 , NM_021163.4
Accession Version	NM_021163.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	RB-associated KRAB zinc finger protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF226869.1, AL711327.1, AK093275.1, CD238907.1 and DB363461.1. This sequence is a reference standard in the RefSeqGene project. On Aug 25, 2006 this sequence version replaced NM_021163.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.171803.1, SRR1660805.219282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066986.1
CDS	520..2664
Translation	MNTLQGPVSFKDVAVDFTQEEWQQLDPDEKITYRDVMLENYSHLVSVGYDTTKPNVIIKLEQGEEPWIMGGEFPCQHSPEAWRVDDLIERIQENEDKHSRQAACINSKTLTEEKENTFSQIYMETSLVPSSIIAHNCVSCGKNLESISQLISSDGSYARTKPDECNECGKTYHGEKMCEFNQNGDTYSHNEENILQKISILEKPFEYNECMEALDNEAVFIAHKRAYIGEKPYEWNDSGPDFIQMSNFNAYQRSQMEMKPFECSECGKSFCKKSKFIIHQRAHTGEKPYECNVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHLRTHSGEKPYECSECGKTFCQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALSDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTHTGEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECNECGKTFNLNSAFIRHRKVHTEEKSHECSECGKFSQLYLTDHHTAHLEEKPYECNECGKTFLVNSAFDGHQPLPKGEKSYECNVCGKLFNELSYYTEHYRSHSEEKPYGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFSRMSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHRRGNMNVLDVENL

Target ORF information:

RefSeq Version	NM_021163.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021163.3

ORF Insert Sequence:

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBAK ( NM_001204456.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191385.1 Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.

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CloneID	OHu10623
Clone ID Related Accession (Same CDS sequence)	NM_001204456.2 , NM_021163.3 , NM_001204456.1 , NM_021163.4
Accession Version	NM_001204456.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	RB-associated KRAB zinc finger protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293069.1 and AC092032.5. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK293069.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191385.1
CDS	325..2469
Translation	MNTLQGPVSFKDVAVDFTQEEWQQLDPDEKITYRDVMLENYSHLVSVGYDTTKPNVIIKLEQGEEPWIMGGEFPCQHSPEAWRVDDLIERIQENEDKHSRQAACINSKTLTEEKENTFSQIYMETSLVPSSIIAHNCVSCGKNLESISQLISSDGSYARTKPDECNECGKTYHGEKMCEFNQNGDTYSHNEENILQKISILEKPFEYNECMEALDNEAVFIAHKRAYIGEKPYEWNDSGPDFIQMSNFNAYQRSQMEMKPFECSECGKSFCKKSKFIIHQRAHTGEKPYECNVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHLRTHSGEKPYECSECGKTFCQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALSDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTHTGEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECNECGKTFNLNSAFIRHRKVHTEEKSHECSECGKFSQLYLTDHHTAHLEEKPYECNECGKTFLVNSAFDGHQPLPKGEKSYECNVCGKLFNELSYYTEHYRSHSEEKPYGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFSRMSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHRRGNMNVLDVENL

Target ORF information:

RefSeq Version	NM_001204456.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204456.1

ORF Insert Sequence:

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBAK ( NM_021163.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066986.1 Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 1, mRNA.

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CloneID	OHu10623
Clone ID Related Accession (Same CDS sequence)	NM_001204456.2 , NM_021163.3 , NM_001204456.1 , NM_021163.4
Accession Version	NM_021163.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	RB-associated KRAB zinc finger protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092032.5, AF226869.1, AL711327.1, AK093275.1, CD238907.1 and DB363461.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_021163.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.171803.1, SRR1660805.219282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396912.2/ ENSP00000380120.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066986.1
CDS	582..2726
Translation	MNTLQGPVSFKDVAVDFTQEEWQQLDPDEKITYRDVMLENYSHLVSVGYDTTKPNVIIKLEQGEEPWIMGGEFPCQHSPEAWRVDDLIERIQENEDKHSRQAACINSKTLTEEKENTFSQIYMETSLVPSSIIAHNCVSCGKNLESISQLISSDGSYARTKPDECNECGKTYHGEKMCEFNQNGDTYSHNEENILQKISILEKPFEYNECMEALDNEAVFIAHKRAYIGEKPYEWNDSGPDFIQMSNFNAYQRSQMEMKPFECSECGKSFCKKSKFIIHQRAHTGEKPYECNVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHLRTHSGEKPYECSECGKTFCQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALSDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTHTGEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECNECGKTFNLNSAFIRHRKVHTEEKSHECSECGKFSQLYLTDHHTAHLEEKPYECNECGKTFLVNSAFDGHQPLPKGEKSYECNVCGKLFNELSYYTEHYRSHSEEKPYGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFSRMSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHRRGNMNVLDVENL

Target ORF information:

RefSeq Version	NM_021163.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021163.4

ORF Insert Sequence:

ATGAACACAT TGCAGGGGCC AGTGTCATTC AAAGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AGCTGGACCC TGATGAGAAG ATAACTTACA GGGATGTGAT GTTGGAGAAC 
TATAGCCATC TAGTTTCTGT GGGATATGAT ACCACCAAGC CAAACGTCAT CATTAAGTTG 
GAGCAGGGAG AGGAGCCGTG GATAATGGGA GGTGAATTTC CATGTCAACA TAGTCCAGAA 
GCCTGGAGAG TTGATGACCT GATAGAGAGA ATCCAAGAAA ACGAAGACAA ACATTCAAGG 
CAAGCTGCTT GTATCAATAG CAAAACCCTG ACTGAAGAGA AAGAGAATAC ATTTAGTCAA 
ATTTACATGG AAACAAGCCT TGTTCCTTCA AGCATAATAG CTCATAATTG TGTCTCATGT 
GGAAAGAATT TAGAATCTAT TTCGCAATTA ATTAGTAGTG ATGGAAGCTA TGCTAGGACA 
AAACCTGATG AGTGTAATGA ATGTGGGAAA ACATATCATG GAGAGAAAAT GTGTGAATTT 
AATCAAAATG GGGATACCTA TTCTCACAAT GAAGAAAATA TTCTTCAGAA AATTAGTATT 
TTGGAGAAAC CCTTTGAATA TAATGAATGC ATGGAAGCCT TAGACAATGA GGCTGTTTTT 
ATTGCTCATA AGAGAGCTTA CATAGGGGAG AAGCCCTATG AGTGGAATGA TTCTGGACCA 
GACTTCATAC AGATGTCAAA TTTTAATGCA TATCAGAGAT CACAAATGGA AATGAAGCCC 
TTTGAATGCA GTGAATGTGG AAAATCCTTC TGTAAAAAGT CAAAATTCAT CATCCACCAG 
AGGGCTCACA CAGGAGAGAA ACCTTATGAA TGTAATGTAT GTGGGAAATC CTTCAGCCAA 
AAGGGAACCC TCACTGTACA TCGGAGATCA CACTTAGAGG AGAAGCCCTA TAAATGTAAT 
GAATGTGGGA AAACCTTTTG TCAGAAGTTA CACCTCACTC AACACCTAAG AACTCATTCA 
GGAGAGAAAC CCTACGAATG TAGCGAATGT GGGAAAACCT TCTGCCAAAA GACACATCTC 
ACCCTGCACC AGAGGAATCA TTCAGGAGAG AGGCCCTATC CATGTAACGA ATGTGGGAAA 
TCCTTCTCCC GCAAGTCTGC TCTCAGTGAC CATCAGAGAA CTCACACGGG AGAGAAGCTT 
TATAAATGTA ATGAATGTGG GAAATCCTAC TACCGAAAGT CTACTCTGAT TACACATCAG 
AGAACACACA CGGGAGAGAA GCCCTATCAG TGTAGCGAGT GTGGGAAATT CTTTTCTCGG 
GTGTCATACC TCACTATACA TTATAGAAGT CATTTAGAAG AGAAACCCTA TGAATGTAAT 
GAATGTGGCA AAACCTTCAA TTTAAATTCA GCCTTCATTA GACATCGGAA AGTACACACA 
GAAGAGAAAT CCCATGAATG TAGTGAATGT GGAAAGTTCT CTCAGTTGTA TCTCACCGAC 
CATCATACAG CTCATTTAGA AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAACCTTC 
CTTGTAAATT CAGCCTTCGA TGGGCACCAG CCACTTCCAA AAGGGGAGAA ATCCTATGAA 
TGTAATGTAT GTGGAAAGTT ATTCAATGAG TTGTCATACT ATACTGAACA TTATAGAAGT 
CATTCAGAAG AGAAACCTTA TGGATGTAGC GAATGTGGGA AAACCTTTTC CCATAATTCA 
TCCCTCTTCA GACATCAAAG AGTACACACA GGCGAGAAAC CCTATGAATG TTACGAATGT 
GGAAAATTCT TCTCTCAGAA ATCATATCTC ACTATACATC ATCGAATTCA TTCAGGAGAG 
AAACCCTATG AATGTAGTAA ATGTGGAAAA GTCTTCTCTC GGATGTCAAA CCTCACTGTC 
CACTACAGAA GCCATTCAGG AGAGAAACCC TATGAATGTA ATGAATGTGG GAAAGTCTTT 
TCTCAGAAGT CATACCTCAC TGTACACTAT AGAACTCATT CAGGAGAGAA ACCCTATGAA 
TGTAACGAGT GTGGGAAAAA ATTCCACCAC AGATCAGCCT TCAATAGCCA TCAGAGAATT 
CATAGAAGAG GAAATATGAA CGTACTTGAT GTGGAAAATC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Review for the regulatory functions of KRAB zinc finger proteins in embryonic development and tumorgenesis of higher vertebrates].
Yi chuan = Hereditas32(5)431-6(2010 May)
Ma ZF,Yang D,He FC,Jiang Y

Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
Gut58(8)1078-83(2009 Aug)
Trynka G,Zhernakova A,Romanos J,Franke L,Hunt KA,Turner G,Bruinenberg M,Heap GA,Platteel M,Ryan AW,de Kovel C,Holmes GK,Howdle PD,Walters JR,Sanders DS,Mulder CJ,Mearin ML,Verbeek WH,Trimble V,Stevens FM,Kelleher D,Barisani D,Bardella MT,McManus R,van Heel DA,Wijmenga C

A census of human transcription factors: function, expression and evolution.
Nature reviews. Genetics10(4)252-63(2009 04)
Vaquerizas JM,Kummerfeld SK,Teichmann SA,Luscombe NM

Identification of candidate genes for human pituitary development by EST analysis.
BMC genomics10109(2009 Mar)
Ma Y,Qi X,Du J,Song S,Feng D,Qi J,Zhu Z,Zhang X,Xiao H,Han Z,Hao X

Examination of chromosome 7p22 candidate genes RBaK, PMS2 and GNA12 in familial hyperaldosteronism type II.
Clinical and experimental pharmacology & physiology35(4)380-5(2008 Apr)
Jeske YW,So A,Kelemen L,Sukor N,Willys C,Bulmer B,Gordon RD,Duffy D,Stowasser M

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RBAK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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