CWC22 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CWC22
Entrez Gene ID	57703
Full Name	CWC22 spliceosome associated protein homolog
Synonyms	EIF4GL,NCM,fSAPb
General protein information	Preferred Names CWC22 spliceosome associated protein homolog Names pre-mRNA-splicing factor CWC22 homolog CWC22 homolog, spliceosome-associated protein CWC22 spliceosome-associated protein functional spliceosome-associated protein b nucampholin homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q31.3
Disorder MIM:	615186

mRNA and Protein(s)

mRNA	Protein	Name
XM_005246726.3	XP_005246783.1	pre-mRNA-splicing factor CWC22 homolog isoform X1
NM_001376032.1	NP_001362961.1	pre-mRNA-splicing factor CWC22 homolog isoform b
NM_001376033.1	NP_001362962.1	pre-mRNA-splicing factor CWC22 homolog isoform c
NM_020943.2	NP_065994.1	pre-mRNA-splicing factor CWC22 homolog
NM_001376029.1	NP_001362958.1	pre-mRNA-splicing factor CWC22 homolog isoform a
NM_001376030.1	NP_001362959.1	pre-mRNA-splicing factor CWC22 homolog isoform a
NM_020943.3	NP_065994.1	pre-mRNA-splicing factor CWC22 homolog isoform a
XM_005246726.2	XP_005246783.1	pre-mRNA-splicing factor CWC22 homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CWC22	NP_065994.1
Mus musculus (house mouse)	Cwc22	NP_085037.2
Eremothecium gossypii	AGOS_AGL355W	NP_986312.1
Rattus norvegicus (Norway rat)	Cwc22	XP_006234487.1
Gallus gallus (chicken)	CWC22	NP_001026421.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007874	XP_317618.4
Schizosaccharomyces pombe (fission yeast)	cwf22	NP_596256.4
Bos taurus (cattle)	CWC22	XP_005202345.1
Danio rerio (zebrafish)	cwc22	NP_001071037.1
Kluyveromyces lactis	KLLA0C14025g	XP_452827.1
	CWC22	XP_001100369.1
Canis lupus familiaris (dog)	CWC22	XP_005640430.1
Saccharomyces cerevisiae (baker's yeast)	CWC22	NP_011794.3
Pan troglodytes (chimpanzee)	CWC22	XP_001157639.2
Rattus norvegicus (Norway rat)	LOC500684	NP_001041424.1
Xenopus tropicalis (tropical clawed frog)	cwc22	XP_004917800.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the human activated spliceosome in three conformational states.
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell research(2018 Jan)

Structure of a human catalytic step I spliceosome.
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (New York, N.Y.)(2018 Jan)

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

Crystal structure of the human eIF4AIII-CWC22 complex shows how a DEAD-box protein is inhibited by a MIF4G domain.
Buchwald G, Sch?ssler S, Basquin C, Le Hir H, Conti E
Proceedings of the National Academy of Sciences of the United States of America110(48)E4611-8(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The binding mode of CWC22 to eIF4AIII reveals a facet of how MIF4G domains use their versatile structural frameworks to activate or inhibit DEAD-box proteins.
Title: Crystal structure of the human eIF4AIII-CWC22 complex shows how a DEAD-box protein is inhibited by a MIF4G domain.

The essential splicing factor CWC22 has acquired functions in exon junction complex assembly and nonsense-mediated decay during evolution from single-celled to complex eukaryotes.
Title: Human spliceosomal protein CWC22 plays a role in coupling splicing to exon junction complex deposition and nonsense-mediated decay.

CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly
Title: Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.

CWC22 connects pre-mRNA splicing and exon junction complex assembly.
Title: CWC22 connects pre-mRNA splicing and exon junction complex assembly.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following CWC22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CWC22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28882	NM_020943.3 Latest version!	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28882	XM_005246726.3 Latest version!	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu109049	NM_001376032.1 Latest version!	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu109064	NM_001376033.1 Latest version!	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu28882	NM_001376030.1 Latest version!	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu28882	NM_001376029.1 Latest version!	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu28882	NM_020943.2	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28882	XM_005246726.2	Homo sapiens CWC22 homolog, spliceosome-associated protein (CWC22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CWC22 ( NM_020943.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065994.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), mRNA.

Price & Availability↑

CloneID	OHu28882
Clone ID Related Accession (Same CDS sequence)	XM_005246726.3 , NM_020943.2 , NM_001376029.1 , NM_001376030.1 , NM_020943.3 , XM_005246726.2
Accession Version	NM_020943.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC031216.1 and AB046824.1. On Jul 25, 2007 this sequence version replaced NM_020943.1. ##Evidence-Data-START## Transcript exon combination :: BC031216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065994.1
CDS	301..3027
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	NM_020943.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020943.2

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( NM_020943.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065994.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28882
Clone ID Related Accession (Same CDS sequence)	XM_005246726.3 , NM_020943.2 , NM_001376029.1 , NM_001376030.1 , NM_020943.3 , XM_005246726.2
Accession Version	NM_020943.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-06
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC068194.6, BC031216.1, AB046824.1 and AC096587.1. On Nov 24, 2018 this sequence version replaced NM_020943.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000410053.8/ ENSP00000387006.3 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065994.1
CDS	545..3271
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	NM_020943.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020943.3

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( XM_005246726.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246783.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu28882
Clone ID Related Accession (Same CDS sequence)	XM_005246726.3 , NM_020943.2 , NM_001376029.1 , NM_001376030.1 , NM_020943.3 , XM_005246726.2
Accession Version	XM_005246726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005246726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246783.1
CDS	341..3067
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	XM_005246726.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246726.3

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( XM_005246726.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246783.1 Homo sapiens CWC22 homolog, spliceosome-associated protein (CWC22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu28882
Clone ID Related Accession (Same CDS sequence)	XM_005246726.3 , NM_020943.2 , NM_001376029.1 , NM_001376030.1 , NM_020943.3 , XM_005246726.2
Accession Version	XM_005246726.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246783.1
CDS	404..3130
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	XM_005246726.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 homolog, spliceosome-associated protein (CWC22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246726.2

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( NM_001376032.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362961.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu109049
Clone ID Related Accession (Same CDS sequence)	NM_001376032.1
Accession Version	NM_001376032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-06
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC068194.6 and AC096587.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138513.246414.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAAAATT ACTTTGATTA CAGCAGATCA GACTATGAGC ATTCAAGAAG AGGACGTTCT 
TATGATAGTA GCATGGAGTC ACGAAACAGG GACCGAGAAA AACGCAGAGA AAGAGAAAGA 
GATACGGATC GGAAAAGGTC TCGGAAATCC CCATCTCCTG GGAGGAGAAA CCCAGAAACA 
TCAGTAACTC AGAGTTCCTC TGCTCAGGAT GAACCTGCTA CAAAGAAAAA GAAAGATGAG 
CTGGATCCTC TTCTTACTCG CACTGGTGGA GCATATATTC CCCCTGCAAA GCTCAGGATG 
ATGCAGGAAC AGATTACAGA TAAAAACAGC TTAGCATACC AGAGGATGAG TTGGGAGGCC 
CTGAAGAAGT CAATTAATGG CCTTATCAAC AAAGTCAACA TTTCCAACAT AAGTATTATT 
ATTCAAGAGC TTCTTCAAGA AAATATAGTT AGAGGAAGAG GACTGCTGTC CAGGTCTGTT 
TTGCAAGCAC AGAGTGCTTC TCCAATCTTC ACCCATGTTT ATGCAGCATT AGTGGCAATT 
ATCAACTCAA AATTTCCACA AATTGGAGAA TTAATCCTCA AAAGGTTAAT TCTTAATTTT 
CGAAAAGGCT ATCGAAGAAA TGACAAGCAA CTTTGCCTGA CTGCTTCAAA ATTTGTGGCG 
CATCTTATTA ACCAAAATGT GGCACACGAA GTATTATGCT TAGAGATGCT CACTTTGCTC 
CTGGAAAGAC CAACAGATGA TAGCGTTGAA GTAGCTATTG GTTTTCTTAA GGAATGTGGC 
CTCAAATTAA CACAAGTGTC ACCAAGAGGA ATCAATGCTA TATTTGAACG CCTTCGAAAC 
ATTCTGCATG AGTCTGAAAT TGACAAAAGA GTTCAATATA TGATTGAAGT GATGTTTGCT 
GTACGGAAAG ATGGATTCAA GGACCACCCC ATTATCCTAG AAGGTCTTGA TTTGGTGGAA 
GAAGATGATC AATTCACTCA TATGCTCCCT CTGGAGGATG ACTATAATCC AGAAGATGTT 
CTTAATGTTT TCAAGATGGA TCCTAATTTT ATGGAGAATG AAGAGAAGTA CAAAGCTATT 
AAGAAAGAAA TTCTTGATGA GGGAGATACT GACTCGAACA CAGACCAGGA TGCTGGGAGT 
AGTGAAGAGG ACGAGGAAGA AGAAGAGGAA GAGGGAGAAG AAGATGAAGA AGGACAAAAA 
GTAACTATTC ATGACAAAAC AGAAATTAAC CTGGTCTCAT TTCGTCGTAC AATTTATCTT 
GCTATTCAGT CAAGTTTAGA TTTTGAAGAA TGTGCTCACA AATTGCTGAA AATGGAGTTT 
CCTGAAAGCC AAACAAAAGA ACTCTGCAAC ATGATACTTG ATTGCTGTGC CCAACAGAGG 
ACATACGAAA AATTTTTTGG CTTATTAGCT GGGCGATTTT GCATGCTAAA GAAAGAGTAC 
ATGGAATCCT TTGAAGGTAT ATTCAAAGAA CAGTATGATA CCATCCATCG CTTGGAAACA 
AACAAGTTGC GAAATGTTGC TAAGATGTTT GCTCACCTTT TATACACTGA TTCACTTCCA 
TGGAGTGTTC TTGAATGTAT AAAACTGAGT GAAGAAACCA CTACATCATC CAGTAGAATT 
TTTGTCAAAA TATTTTTCCA GGAACTGTGT GAATACATGG GTCTTCCTAA ACTTAATGCA 
AGATTAAAGG ATGAAACTCT GCAGCCATTC TTTGAAGGAT TATTACCCCG AGATAATCCA 
AGAAACACTC GGTTTGCCAT CAACTTCTTT ACTTCTATAG GTCTTGGAGG TTTAACGGAT 
GAACTGCGGG AGCATCTCAA AAATACACCA AAGGTCATTG TGGCGCAGAA ACCAGATGTT 
GAGCAAAATA AATCCTCCCC ATCCTCTTCC TCTTCAGCGT CCTCCTCTTC AGAGTCTGAC 
TCATCCGACT CTGATTCTGA CAGCAGTGAT AGCAGTTCAG AGTCTTCCAG TGAAGAGAGC 
GACTCTTCAT CCATCAGTAG TCATAGCTCT GCCTCAGCTA ATGATGTAAG AAAGAAGGGA 
CATGGGAAGA CCAGAAGTAA AGAGGTAGAT AAATTGATCA GAAACCAGCA AACAAATGAT 
AGGAAACAAA AAGAAAGAAG ACAAGAACAC GGGCACCAGG AAACAAGGAC TGAGAGAGAA 
AGAAGGTCAG AAAAACACAG AGATCAAAAT TCAAGTGGTT CAAATTGGAG AGATCCTATA 
ACAAAGTACA CATCAGACAA AGATGTTCCT TCTGAACGAA ATAACTACAG TAGAGTTGCG 
AATGACAGAG ACCAAGAAAT GCATATAGAT TTGGAAAATA AGCATGGTGA TCCCAAAAAG 
AAGAGAGGAG AGAGAAGAAA TTCTTTTTCT GAAAATGAGA AGCATACACA CCGAATTAAA 
GACAGTGAAA ATTTCAGAAG AAAAGATAGA TCAAAGTCAA AGGAAATGAA TAGAAAGCAC 
TCAGGCTCAA GAAGTGATGA AGATAGATAT CAAAATGGTG CCGAGAGACG ATGGGAAAAA 
TCTAGCAGAT ACTCTGAACA ATCCAGAGAA TCAAAGAAAA ATCAGGACCG GCGAAGAGAA 
AAGTCTCCAG CAAAACAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362961.1
CDS	151..2754
Translation	MKNYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	NM_001376032.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376032.1

ORF Insert Sequence:

ATGAAAAATT ACTTTGATTA CAGCAGATCA GACTATGAGC ATTCAAGAAG AGGACGTTCT 
TATGATAGTA GCATGGAGTC ACGAAACAGG GACCGAGAAA AACGCAGAGA AAGAGAAAGA 
GATACGGATC GGAAAAGGTC TCGGAAATCC CCATCTCCTG GGAGGAGAAA CCCAGAAACA 
TCAGTAACTC AGAGTTCCTC TGCTCAGGAT GAACCTGCTA CAAAGAAAAA GAAAGATGAG 
CTGGATCCTC TTCTTACTCG CACTGGTGGA GCATATATTC CCCCTGCAAA GCTCAGGATG 
ATGCAGGAAC AGATTACAGA TAAAAACAGC TTAGCATACC AGAGGATGAG TTGGGAGGCC 
CTGAAGAAGT CAATTAATGG CCTTATCAAC AAAGTCAACA TTTCCAACAT AAGTATTATT 
ATTCAAGAGC TTCTTCAAGA AAATATAGTT AGAGGAAGAG GACTGCTGTC CAGGTCTGTT 
TTGCAAGCAC AGAGTGCTTC TCCAATCTTC ACCCATGTTT ATGCAGCATT AGTGGCAATT 
ATCAACTCAA AATTTCCACA AATTGGAGAA TTAATCCTCA AAAGGTTAAT TCTTAATTTT 
CGAAAAGGCT ATCGAAGAAA TGACAAGCAA CTTTGCCTGA CTGCTTCAAA ATTTGTGGCG 
CATCTTATTA ACCAAAATGT GGCACACGAA GTATTATGCT TAGAGATGCT CACTTTGCTC 
CTGGAAAGAC CAACAGATGA TAGCGTTGAA GTAGCTATTG GTTTTCTTAA GGAATGTGGC 
CTCAAATTAA CACAAGTGTC ACCAAGAGGA ATCAATGCTA TATTTGAACG CCTTCGAAAC 
ATTCTGCATG AGTCTGAAAT TGACAAAAGA GTTCAATATA TGATTGAAGT GATGTTTGCT 
GTACGGAAAG ATGGATTCAA GGACCACCCC ATTATCCTAG AAGGTCTTGA TTTGGTGGAA 
GAAGATGATC AATTCACTCA TATGCTCCCT CTGGAGGATG ACTATAATCC AGAAGATGTT 
CTTAATGTTT TCAAGATGGA TCCTAATTTT ATGGAGAATG AAGAGAAGTA CAAAGCTATT 
AAGAAAGAAA TTCTTGATGA GGGAGATACT GACTCGAACA CAGACCAGGA TGCTGGGAGT 
AGTGAAGAGG ACGAGGAAGA AGAAGAGGAA GAGGGAGAAG AAGATGAAGA AGGACAAAAA 
GTAACTATTC ATGACAAAAC AGAAATTAAC CTGGTCTCAT TTCGTCGTAC AATTTATCTT 
GCTATTCAGT CAAGTTTAGA TTTTGAAGAA TGTGCTCACA AATTGCTGAA AATGGAGTTT 
CCTGAAAGCC AAACAAAAGA ACTCTGCAAC ATGATACTTG ATTGCTGTGC CCAACAGAGG 
ACATACGAAA AATTTTTTGG CTTATTAGCT GGGCGATTTT GCATGCTAAA GAAAGAGTAC 
ATGGAATCCT TTGAAGGTAT ATTCAAAGAA CAGTATGATA CCATCCATCG CTTGGAAACA 
AACAAGTTGC GAAATGTTGC TAAGATGTTT GCTCACCTTT TATACACTGA TTCACTTCCA 
TGGAGTGTTC TTGAATGTAT AAAACTGAGT GAAGAAACCA CTACATCATC CAGTAGAATT 
TTTGTCAAAA TATTTTTCCA GGAACTGTGT GAATACATGG GTCTTCCTAA ACTTAATGCA 
AGATTAAAGG ATGAAACTCT GCAGCCATTC TTTGAAGGAT TATTACCCCG AGATAATCCA 
AGAAACACTC GGTTTGCCAT CAACTTCTTT ACTTCTATAG GTCTTGGAGG TTTAACGGAT 
GAACTGCGGG AGCATCTCAA AAATACACCA AAGGTCATTG TGGCGCAGAA ACCAGATGTT 
GAGCAAAATA AATCCTCCCC ATCCTCTTCC TCTTCAGCGT CCTCCTCTTC AGAGTCTGAC 
TCATCCGACT CTGATTCTGA CAGCAGTGAT AGCAGTTCAG AGTCTTCCAG TGAAGAGAGC 
GACTCTTCAT CCATCAGTAG TCATAGCTCT GCCTCAGCTA ATGATGTAAG AAAGAAGGGA 
CATGGGAAGA CCAGAAGTAA AGAGGTAGAT AAATTGATCA GAAACCAGCA AACAAATGAT 
AGGAAACAAA AAGAAAGAAG ACAAGAACAC GGGCACCAGG AAACAAGGAC TGAGAGAGAA 
AGAAGGTCAG AAAAACACAG AGATCAAAAT TCAAGTGGTT CAAATTGGAG AGATCCTATA 
ACAAAGTACA CATCAGACAA AGATGTTCCT TCTGAACGAA ATAACTACAG TAGAGTTGCG 
AATGACAGAG ACCAAGAAAT GCATATAGAT TTGGAAAATA AGCATGGTGA TCCCAAAAAG 
AAGAGAGGAG AGAGAAGAAA TTCTTTTTCT GAAAATGAGA AGCATACACA CCGAATTAAA 
GACAGTGAAA ATTTCAGAAG AAAAGATAGA TCAAAGTCAA AGGAAATGAA TAGAAAGCAC 
TCAGGCTCAA GAAGTGATGA AGATAGATAT CAAAATGGTG CCGAGAGACG ATGGGAAAAA 
TCTAGCAGAT ACTCTGAACA ATCCAGAGAA TCAAAGAAAA ATCAGGACCG GCGAAGAGAA 
AAGTCTCCAG CAAAACAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( NM_001376033.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362962.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu109064
Clone ID Related Accession (Same CDS sequence)	NM_001376033.1
Accession Version	NM_001376033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-06
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC068194.6 and AC096587.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.195758.1, SRR7410570.210736.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362962.1
CDS	151..1971
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDD

Target ORF information:

RefSeq Version	NM_001376033.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376033.1

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( NM_001376029.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362958.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28882
Clone ID Related Accession (Same CDS sequence)	XM_005246726.3 , NM_020943.2 , NM_001376029.1 , NM_001376030.1 , NM_020943.3 , XM_005246726.2
Accession Version	NM_001376029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-06
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC068194.6 and AC096587.1. On Nov 7, 2019 this sequence version replaced XM_005246726.3. Transcript Variant: This variant (2), as well as variants 1 and 3, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057826.1, AK025635.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362958.1
CDS	151..2877
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	NM_001376029.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376029.1

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CWC22 ( NM_001376030.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362959.1 Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28882
Clone ID Related Accession (Same CDS sequence)	XM_005246726.3 , NM_020943.2 , NM_001376029.1 , NM_001376030.1 , NM_020943.3 , XM_005246726.2
Accession Version	NM_001376030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-06
Organism	Homo sapiens(human)
Product	pre-mRNA-splicing factor CWC22 homolog isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC068194.6 and AC096587.1. Transcript Variant: This variant (3). as well as variants 1 and 2, encodes isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.163994.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362959.1
CDS	290..3016
Translation	MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Target ORF information:

RefSeq Version	NM_001376030.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CWC22 spliceosome associated protein homolog (CWC22), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376030.1

ORF Insert Sequence:

ATGAAAAGTA GTGTGGCACA GATAAAACCT TCTTCTGGTC ATGACAGAAG GGAAAACCTT 
AATTCATATC AGAGGAACTC CTCTCCAGAA GACAGATATG AAGAACAAGA ACGATCCCCC 
CGGGATAGAG ATTACTTTGA TTACAGCAGA TCAGACTATG AGCATTCAAG AAGAGGACGT 
TCTTATGATA GTAGCATGGA GTCACGAAAC AGGGACCGAG AAAAACGCAG AGAAAGAGAA 
AGAGATACGG ATCGGAAAAG GTCTCGGAAA TCCCCATCTC CTGGGAGGAG AAACCCAGAA 
ACATCAGTAA CTCAGAGTTC CTCTGCTCAG GATGAACCTG CTACAAAGAA AAAGAAAGAT 
GAGCTGGATC CTCTTCTTAC TCGCACTGGT GGAGCATATA TTCCCCCTGC AAAGCTCAGG 
ATGATGCAGG AACAGATTAC AGATAAAAAC AGCTTAGCAT ACCAGAGGAT GAGTTGGGAG 
GCCCTGAAGA AGTCAATTAA TGGCCTTATC AACAAAGTCA ACATTTCCAA CATAAGTATT 
ATTATTCAAG AGCTTCTTCA AGAAAATATA GTTAGAGGAA GAGGACTGCT GTCCAGGTCT 
GTTTTGCAAG CACAGAGTGC TTCTCCAATC TTCACCCATG TTTATGCAGC ATTAGTGGCA 
ATTATCAACT CAAAATTTCC ACAAATTGGA GAATTAATCC TCAAAAGGTT AATTCTTAAT 
TTTCGAAAAG GCTATCGAAG AAATGACAAG CAACTTTGCC TGACTGCTTC AAAATTTGTG 
GCGCATCTTA TTAACCAAAA TGTGGCACAC GAAGTATTAT GCTTAGAGAT GCTCACTTTG 
CTCCTGGAAA GACCAACAGA TGATAGCGTT GAAGTAGCTA TTGGTTTTCT TAAGGAATGT 
GGCCTCAAAT TAACACAAGT GTCACCAAGA GGAATCAATG CTATATTTGA ACGCCTTCGA 
AACATTCTGC ATGAGTCTGA AATTGACAAA AGAGTTCAAT ATATGATTGA AGTGATGTTT 
GCTGTACGGA AAGATGGATT CAAGGACCAC CCCATTATCC TAGAAGGTCT TGATTTGGTG 
GAAGAAGATG ATCAATTCAC TCATATGCTC CCTCTGGAGG ATGACTATAA TCCAGAAGAT 
GTTCTTAATG TTTTCAAGAT GGATCCTAAT TTTATGGAGA ATGAAGAGAA GTACAAAGCT 
ATTAAGAAAG AAATTCTTGA TGAGGGAGAT ACTGACTCGA ACACAGACCA GGATGCTGGG 
AGTAGTGAAG AGGACGAGGA AGAAGAAGAG GAAGAGGGAG AAGAAGATGA AGAAGGACAA 
AAAGTAACTA TTCATGACAA AACAGAAATT AACCTGGTCT CATTTCGTCG TACAATTTAT 
CTTGCTATTC AGTCAAGTTT AGATTTTGAA GAATGTGCTC ACAAATTGCT GAAAATGGAG 
TTTCCTGAAA GCCAAACAAA AGAACTCTGC AACATGATAC TTGATTGCTG TGCCCAACAG 
AGGACATACG AAAAATTTTT TGGCTTATTA GCTGGGCGAT TTTGCATGCT AAAGAAAGAG 
TACATGGAAT CCTTTGAAGG TATATTCAAA GAACAGTATG ATACCATCCA TCGCTTGGAA 
ACAAACAAGT TGCGAAATGT TGCTAAGATG TTTGCTCACC TTTTATACAC TGATTCACTT 
CCATGGAGTG TTCTTGAATG TATAAAACTG AGTGAAGAAA CCACTACATC ATCCAGTAGA 
ATTTTTGTCA AAATATTTTT CCAGGAACTG TGTGAATACA TGGGTCTTCC TAAACTTAAT 
GCAAGATTAA AGGATGAAAC TCTGCAGCCA TTCTTTGAAG GATTATTACC CCGAGATAAT 
CCAAGAAACA CTCGGTTTGC CATCAACTTC TTTACTTCTA TAGGTCTTGG AGGTTTAACG 
GATGAACTGC GGGAGCATCT CAAAAATACA CCAAAGGTCA TTGTGGCGCA GAAACCAGAT 
GTTGAGCAAA ATAAATCCTC CCCATCCTCT TCCTCTTCAG CGTCCTCCTC TTCAGAGTCT 
GACTCATCCG ACTCTGATTC TGACAGCAGT GATAGCAGTT CAGAGTCTTC CAGTGAAGAG 
AGCGACTCTT CATCCATCAG TAGTCATAGC TCTGCCTCAG CTAATGATGT AAGAAAGAAG 
GGACATGGGA AGACCAGAAG TAAAGAGGTA GATAAATTGA TCAGAAACCA GCAAACAAAT 
GATAGGAAAC AAAAAGAAAG AAGACAAGAA CACGGGCACC AGGAAACAAG GACTGAGAGA 
GAAAGAAGGT CAGAAAAACA CAGAGATCAA AATTCAAGTG GTTCAAATTG GAGAGATCCT 
ATAACAAAGT ACACATCAGA CAAAGATGTT CCTTCTGAAC GAAATAACTA CAGTAGAGTT 
GCGAATGACA GAGACCAAGA AATGCATATA GATTTGGAAA ATAAGCATGG TGATCCCAAA 
AAGAAGAGAG GAGAGAGAAG AAATTCTTTT TCTGAAAATG AGAAGCATAC ACACCGAATT 
AAAGACAGTG AAAATTTCAG AAGAAAAGAT AGATCAAAGT CAAAGGAAAT GAATAGAAAG 
CACTCAGGCT CAAGAAGTGA TGAAGATAGA TATCAAAATG GTGCCGAGAG ACGATGGGAA 
AAATCTAGCA GATACTCTGA ACAATCCAGA GAATCAAAGA AAAATCAGGA CCGGCGAAGA 
GAAAAGTCTC CAGCAAAACA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the human activated spliceosome in three conformational states.
Cell research(2018 Jan)
Zhang X,Yan C,Zhan X,Li L,Lei J,Shi Y

Structure of a human catalytic step I spliceosome.
Science (New York, N.Y.)(2018 Jan)
Zhan X,Yan C,Zhang X,Lei J,Shi Y

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

Crystal structure of the human eIF4AIII-CWC22 complex shows how a DEAD-box protein is inhibited by a MIF4G domain.
Proceedings of the National Academy of Sciences of the United States of America110(48)E4611-8(2013 Nov)
Buchwald G,Sch?ssler S,Basquin C,Le Hir H,Conti E

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CWC22 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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