MYH7B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH7B
Entrez Gene ID	57644
Full Name	myosin heavy chain 7B
Synonyms	MHC14,MYH14
General protein information	Preferred Names myosin heavy chain 7B Names myosin-7B U937-associated antigen antigen MLAA-21 myosin heavy chain 7B, cardiac muscle beta isoform myosin, heavy chain 7B, cardiac muscle, beta myosin, heavy polypeptide 7B, cardiac muscle, beta slow A MYH14
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.22
Summary	The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined. [provided by RefSeq, Mar 2010].
Disorder MIM:	609928

mRNA and Protein(s)

mRNA	Protein	Name
XM_017027987.1	XP_016883476.1	myosin-7B isoform X4
XM_011528948.2	XP_011527250.1	myosin-7B isoform X4
NM_020884.4	NP_065935.3	myosin-7B
NM_020884.5	NP_065935.3	myosin-7B
XM_011528947.2	XP_011527249.1	myosin-7B isoform X4
XM_006723840.3	XP_006723903.1	myosin-7B isoform X3
XM_017027986.1	XP_016883475.1	myosin-7B isoform X1
XM_011528941.2	XP_011527243.1	myosin-7B isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH7B	XP_001160839.2
Gallus gallus (chicken)	MYH7B	NP_989918.1
Danio rerio (zebrafish)	myh7bb	XP_005162166.1
Caenorhabditis elegans (roundworm)	myo-3	NP_506065.2
Homo sapiens (human)	MYH7B	NP_065935.2
Bos taurus (cattle)	MYH7B	XP_003586892.2
Mus musculus (house mouse)	Myh7b	NP_001078847.1
Danio rerio (zebrafish)	myh7	XP_005162168.1
Caenorhabditis elegans (roundworm)	myo-2	NP_510092.2
Caenorhabditis elegans (roundworm)	myo-5	NP_505094.1
Rattus norvegicus (Norway rat)	Myh7b	NP_001101264.2
Caenorhabditis elegans (roundworm)	unc-54	NP_493596.1
Canis lupus familiaris (dog)	MYH7B	XP_534397.4
Caenorhabditis elegans (roundworm)	myo-1	NP_492053.1
Xenopus tropicalis (tropical clawed frog)	myh7b	XP_002932939.2

Gene Ontology
Bibliography

Related articles in PubMed

Myosin-7b Promotes Distal Tip Localization of the Intermicrovillar Adhesion Complex.
Weck ML, Crawley SW, Stone CR, Tyska MJ
Current biology : CB26(20)2717-2728(2016 Oct)

Digenic mutational inheritance of the integrin alpha 7 and the myosin heavy chain 7B genes causes congenital myopathy with left ventricular non-compact cardiomyopathy.
Esposito T, Sampaolo S, Limongelli G, Varone A, Formicola D, Diodato D, Farina O, Napolitano F, Pacileo G, Gianfrancesco F, Di Iorio G
Orphanet journal of rare diseases891(2013 Jun)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

Genetic associations for activated partial thromboplastin time and prothrombin time, their gene expression profiles, and risk of coronary artery disease.
Tang W, Schwienbacher C, Lopez LM, Ben-Shlomo Y, Oudot-Mellakh T, Johnson AD, Samani NJ, Basu S, G?gele M, Davies G, Lowe GD, Tregouet DA, Tan A, Pankow JS, Tenesa A, Levy D, Volpato CB, Rumley A, Gow AJ, Minelli C, Yarnell JW, Porteous DJ, Starr JM, Gallacher J, Boerwinkle E, Visscher PM, Pramstaller PP, Cushman M, Emilsson V, Plump AS, Matijevic N, Morange PE, Deary IJ, Hicks AA, Folsom AR
American journal of human genetics91(1)152-62(2012 Jul)

Genome wide association study for plasma levels of natural anticoagulant inhibitors and protein C anticoagulant pathway: the MARTHA project.
Oudot-Mellakh T, Cohen W, Germain M, Saut N, Kallel C, Zelenika D, Lathrop M, Tr?gou?t DA, Morange PE
British journal of haematology157(2)230-9(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The actin-based motor myosin-7b (Myo7b) promotes the accumulation of intermicrovillar adhesion complex (IMAC components at microvillar tips. Myo7b is highly enriched at the tips of microvilli in both kidney and intestinal brush borders, and loss of Myo7b in differentiating intestinal epithelial cells disrupts intermicrovillar adhesion and, thus, brush border assembly.
Title: Myosin-7b Promotes Distal Tip Localization of the Intermicrovillar Adhesion Complex.

Digenic mutational inheritance of the integrin alpha 7 and the myosin heavy chain 7B genes causes congenital myopathy with left ventricular non-compact cardiomyopathy. [MYH7B]
Title: Digenic mutational inheritance of the integrin alpha 7 and the myosin heavy chain 7B genes causes congenital myopathy with left ventricular non-compact cardiomyopathy.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Common genetic variants in pre-microRNAs and risk of gallbladder cancer in North Indian population.

Observational study of gene-disease association. (HuGE Navigator)
Title: Modest association of malignant melanoma with the rs910873 and rs1885120 markers on chromosome 20: a population-based study.

The following MYH7B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH7B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28325	NM_020884.5 Latest version!	Homo sapiens myosin heavy chain 7B (MYH7B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu53063	XM_006723840.3 Latest version!	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu70756	XM_011528941.2 Latest version!	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu70760	XM_017027987.1 Latest version!	Homo sapiens myosin, heavy chain 7B, cardiac muscle, beta (MYH7B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu70762	XM_011528948.2 Latest version!	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu70762	XM_011528947.2 Latest version!	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu93095	XM_017027986.1 Latest version!	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu28325	NM_020884.4	Homo sapiens myosin heavy chain 7B (MYH7B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH7B ( NM_020884.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065935.3 Homo sapiens myosin heavy chain 7B (MYH7B), mRNA.

Price & Availability↑

CloneID	OHu28325
Clone ID Related Accession (Same CDS sequence)	NM_020884.4 , NM_020884.5
Accession Version	NM_020884.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	myosin-7B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133324.13, DB484143.2, BC151242.1, AL132825.35 and BC007808.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2014 this sequence version replaced NM_020884.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB040945.2, BC151242.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CACAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065935.3
CDS	384..6335
Translation	MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGYQEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPMNPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVVAAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKHKE

Target ORF information:

RefSeq Version	NM_020884.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020884.4

ORF Insert Sequence:

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CACAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( NM_020884.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065935.3 Homo sapiens myosin heavy chain 7B (MYH7B), mRNA.

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CloneID	OHu28325
Clone ID Related Accession (Same CDS sequence)	NM_020884.4 , NM_020884.5
Accession Version	NM_020884.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	myosin-7B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133324.13, DB484143.2, BC151242.1, AL132825.35 and BC007808.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_020884.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB040945.2, BC151242.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CACAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065935.3
CDS	351..6302
Translation	MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGYQEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPMNPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVVAAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKHKE

Target ORF information:

RefSeq Version	NM_020884.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020884.5

ORF Insert Sequence:

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CACAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( XM_006723840.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723903.1 Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X3, mRNA.

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CloneID	OHu53063
Clone ID Related Accession (Same CDS sequence)	XM_006723840.3
Accession Version	XM_006723840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-7B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CTATCCCTTT CTCCTCAGCA CAAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723903.1
CDS	25..5994
Translation	MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGYQEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPMNPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVVAAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKLSLSPQHKE

Target ORF information:

RefSeq Version	XM_006723840.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723840.3

ORF Insert Sequence:

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CTATCCCTTT CTCCTCAGCA CAAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( XM_011528941.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527243.1 Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X2, mRNA.

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CloneID	OHu70756
Clone ID Related Accession (Same CDS sequence)	XM_011528941.2
Accession Version	XM_011528941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-7B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAGTAAG GGCCGCCTGG ACTCCTCCCC AACCGCAGAC 
CCCGACCTCG GTCCCGGGAG GCCTCTACCG AGACAACCAG TCCATGCTGA TCACCAATTT 
CTGGCAACAA AGACGGGGGG CACCCTTGAG GATCAAATCA TCGAGGCCAA CCCTGCCATG 
GAGGCCTTTG GCAACGCCAA GACCCTGAGG AATGATAACT CCTCCCGCTT TGGCAAGTTC 
ATCCGCATTC ACTTTGGTCC CTCTGGGAAG CTGGCATCCG CGGATATTGA CAGCTATCTC 
CTGGAGAAGT CGCGGGTGAT CTTCCAGTTG CCTGGTGAGC GCAGCTACCA TGTCTACTAC 
CAGATCCTCT CAGGGAGGAA GCCAGAGCTG CAGGACATGC TGCTTCTGTC TATGAACCCC 
TATGACTACC ACTTCTGCAG CCAGGGCGTC ATCACCGTGG ACAACATGAA TGATGGGGAG 
GAGCTCATCG CCACCGACCA TGCCATGGAC ATCCTAGGCT TCAGCGTGGA TGAGAAATGT 
GCCTGCTATA AGATCGTGGG CGCCCTCCTG CACTTTGGCA ACATGAAGTT CAAGCAGAAG 
CAGCGGGAGG AGCAGGCGGA GGCCGATGGC ACTGAGAGTG CTGACAAGGC TGCCTACCTG 
ATGGGGGTCA GCAGTGGGGA CCTCCTCAAA GGCCTTTTGC ACCCCCGGGT GCGTGTAGGG 
AACGAGTACG TGACCAAGGG CCAGAGTGTG GAGCAGGTGG TGTTTGCTGT GGGGGCTCTG 
GCCAAGGCCA CCTATGACCG GCTGTTCAGG TGGCTGGTGT CTCGGATCAA CCAGACCCTG 
GACACAAAGC TGCCCCGGCA GTTCTTCATC GGGGTTCTGG ACATCGCTGG GTTTGAGATC 
TTTGAGTTCA ACAGCTTCGA ACAGCTGTGC ATCAACTTCA CCAATGAGAA ATTGCAGCAG 
TTCTTCAACC AGCACATGTT TGTGCTGGAG CAGGAGGAGT ACAAGCGGGA GGGCATCGAC 
TGGGTCTTCA TCGACTTCGG CCTTGACCTG CAGCCTTGCA TCGACCTCAT CGAGAAGGTT 
AGAAGACTGA GGCTTAGTGA TGTCGGAGGG CCCCACAGCT ACTCGTTCTG TTCCCAGCCA 
CTGGGCATCC TGTCCATCCT GGAGGAGGAA TGCATGTTCC CCAAGGCCTC AGACGCCAGC 
TTCCGGGCCA AGCTCTACGA CAACCACGCG GGGAAGTCAC CCAATTTCCA GCAGCCTCGG 
CCTGACAAGA AGCGCAAGTA CCAGGCCCAC TTCGAGGTGG TCCACTACGC AGGCGTGGTG 
CCTTACAGCA TTGTGGGCTG GCTGGAGAAA AACAAGGATC CCCTGAATGA GACCGTGGTC 
CCCATCTTCC AGAAGTCACA GAATAGGCTC CTGGCGACTC TCTATGAGAA TTATGCGGGC 
TCCTGCTCCA CTGAGCCCCC CAAGTCTGGG GTGAAAGAGA AGCGTAAGAA GGCAGCATCG 
TTCCAGACGG TGTCCCAGCT GCACAAGGAG AACCTCAACA AGCTGATGAC CAACCTGCGG 
GCCACACAGC CCCACTTCGT CCGCTGCATT GTCCCCAACG AGAACAAAAC CCCAGGGGTC 
ATGGATGCCT TCTTGGTGCT ACACCAGCTG CGCTGCAATG GGGTCCTGGA GGGGATCCGG 
ATCTGCCGCC AAGGGTTCCC CAACAGGTTG CTCTACACCG ACTTCCGGCA GCGGTACCGT 
ATCCTGAACC CCAGTGCCAT CCCGGATGAC ACCTTCATGG ACAGCAGGAA GGCCACAGAG 
AAACTGCTGG GCTCGCTGGA CTTGGATCAC ACCCAGTACC AGTTTGGCCA CACCAAGGTG 
TTCTTCAAGG CTGGGCTTCT AGGCGTCCTG GAAGAGCTCC GTGACCAGCG CCTGGCCAAG 
GTGCTGACGC TGCTGCAGGC GCGGAGCCGT GGCCGCCTCA TGCGCCTTGA GTACCAGCGC 
CTGCTGGGAG GCAGGGATGC GCTGTTCACC ATCCAGTGGA ACATCCGTGC CTTCAATGCC 
GTCAAGAACT GGTCATGGAT GAAGCTCTTT TTCAAGATGA AGCCGCTGCT GCGCTCGGCG 
CAGGCTGAGG AGGAGCTGGC GGCCCTGCGG GCAGAGCTGC GGGGGTTGCG AGGGGCGCTG 
GCTGCGGCCG AGGCCAAGCG CCAGGAACTG GAGGAGACGC ACGTCAGCAT CACCCAGGAG 
AAGAATGACC TGGCCCTGCA GCTGCAGGCT GAGCAGGACA ACCTGGCAGA TGCCGAGGAG 
CGCTGCCACT TGCTGATCAA GTCCAAGGTG CAGCTGGAGG GGAAGGTGAA GGAGCTGAGT 
GAGCGGCTGG AGGATGAGGA GGAGGTGAAC GCTGACCTGG CCGCCCGCCG GCGCAAGCTG 
GAGGACGAGT GCACGGAGCT CAAGAAGGAC ATTGATGACC TGGAGCTGAC ACTGGCCAAA 
GCTGAGAAGG AGAAGCAAGC CACTGAGAAC AAGGTGAAGA ACCTGACGGA AGAGATGGCT 
GCGCTGGACG AGTCAGTGGC CCGGCTGACC AAGGAGAAGA AGGCGTTGCA GGAGGCCCAC 
CAACAGGCCC TGGGTGACCT GCAGGCCGAG GAGGACCGTG TGAGCGCGCT GACCAAGGCC 
AAGCTCCGGC TGGAGCAACA GGTGGAGGAC CTGGAATGCT CCCTGGAGCA GGAGAAGAAG 
CTGCGCATGG ACACGGAGCG GGCCAAGCGC AAGCTGGAGG GTGACCTGAA GCTGACGCAG 
GAGTCGGTGG CTGATGCTGC TCAAGACAAG CAGCAGCTGG AGGAGAAGCT CAAGAAGAAG 
GACTCCGAGC TGAGCCAGCT GAGCCTGCGG GTGGAAGACG AGCAGCTCTT GGGGGCCCAG 
ATGCAGAAGA AGATCAAGGA GCTGCAGGCT CGGGCGGAGG AGCTGGAAGA GGAGCTGGAG 
GCAGAGCGGG CAGCCCGGGC CCGCGTGGAG AAGCAGCGTG CAGAGGCGGC GCGGGAGCTG 
GAGGAGCTGA GCGAGCGGCT GGAGGAGGCA GGCGGCGCAT CCGCGGGGCA GCGCGAGGGC 
TGCCGCAAGC GGGAGGCGGA GCTGGGGAGG CTGCGGCGGG AGCTGGAGGA GGCGGCGCTG 
CGGCACGAGG CCACAGTGGC GGCACTGCGG CGCAAGCAGG CGGAGGGCGC GGCGGAGCTG 
GGGGAGCAGG TGGACAGCCT GCAGCGGGTG CGGCAGAAGC TGGAGAAGGA GAAGAGTGAG 
CTGCGCATGG AGGTGGACGA CCTGGCTGCC AACGTGGAGA CTCTGACCCG CGCCAAGGCC 
AGTGCAGAGA AGCTGTGCCG GACCTATGAG GATCAGCTAA GCGAGGCCAA GATCAAGGTG 
GAGGAGCTGC AGCGGCAGCT GGCGGACGCA AGCACGCAGC GTGGGCGACT ACAGACGGAA 
AGCGGGGAGC TGAGTCGCCT GCTAGAGGAG AAGGAGTGTC TGATCAGTCA GCTGAGCCGT 
GGAAAGGCCC TGGCCGCCCA AAGCCTGGAA GAGTTGCGGC GCCAGCTAGA GGAGGAAAGC 
AAGGCCAAGA GTGCCCTGGC CCACGCCGTG CAGGCTCTGC GGCACGACTG TGACCTCCTG 
CGGGAGCAAC ACGAGGAGGA GGCTGAGGCC CAGGCTGAGC TGCAGCGGCT GCTGTCCAAG 
GCCAATGCCG AGGTGGCCCA GTGGAGGAGC AAGTACGAAG CAGATGCCAT CCAGAGGACC 
GAGGAGCTGG AGGAGGCCAA AAAAAAGCTG GCACTGCGGC TGCAGGAGGC AGAGGAGGGC 
GTGGAGGCTG CCAACGCCAA GTGCTCATCG TTGGAGAAGG CCAAGCTGCG GCTACAGACA 
GAGTCAGAGG ATGTAACCCT GGAGCTGGAG CGGGCGACCT CAGCAGCTGC TGCGCTGGAC 
AAGAAGCAGC GGCACTTGGA ACGGGCACTG GAGGAACGGC GGCGGCAGGA GGAGGAGATG 
CAGCGGGAGC TGGAGGCGGC ACAGAGGGAG TCCCGTGGCC TGGGCACCGA GCTCTTCCGG 
CTGCGGCACG GCCACGAGGA GGCACTTGAA GCCCTGGAGA CGCTCAAGCG GGAGAACAAG 
AACCTGCAGG AGGAGATCAG CGACCTCACA GACCAGGTGA GTCTCAGTGG GAAGAGCATC 
CAGGAACTGG AGAAAACCAA GAAGGCGCTG GAAGGCGAGA AGAGTGAGAT CCAGGCTGCA 
CTGGAGGAGG CAGAGGGGGC CCTGGAGCTG GAGGAGACCA AGACGCTGCG GATCCAGCTG 
GAGCTCTCCC AGGTCAAAGC AGAAGTGGAC CGGAAGCTGG CAGAGAAAGA CGAGGAGTGC 
GCTAACCTGA GGCGCAACCA CCAGCGAGCT GTGGAGTCCC TGCAGGCCTC CCTGGATGCA 
GAGACACGGG CCCGCAATGA GGCGCTGCGG CTCAAGAAGA AGATGGAGGG TGACCTCAAC 
GACCTGGAGC TGCAGCTGGG CCATGCCACC CGTCAGGCCA CAGAGGCCCA GGCTGCCACG 
CGGCTGATGC AGGCACAGCT CAAGGAGGAG CAGGCAGGGC GGGACGAGGA GCAGCGGCTG 
GCAGCTGAGC TCCACGAGCA GGCGCAGGCT CTGGAGCGCC GGGCCTCGCT GCTGGCTGCG 
GAGCTGGAGG AGCTGCGGGC TGCCCTGGAG CAGGGCGAGC GCAGCCGGCG ACTGGCAGAG 
CAGGAGCTTT TGGAGGCCAC CGAGCGCCTC AACCTTCTGC ATTCGCAGAA CACAGGCCTC 
CTAAACCAGA AGAAGAAGCT GGAGGCGGAC TTGGCCCAGC TGAGCGGGGA GGTGGAGGAG 
GCTGCACAGG AGAGGCGGGA GGCTGAGGAG AAGGCCAAAA AGGCCATCAC TGATGCGGCC 
ATGATGGCCG AGGAGCTGAA GAAGGAGCAG GACACAAGTG CACACCTGGA ACGGATGAAG 
AAGACGCTGG AGCAGACGGT GCGCGAGCTC CAGGCCCGCC TTGAGGAGGC AGAACAGGCC 
GCCCTCCGTG GCGGGAAGAA GCAGGTGCAG AAGCTGGAGG CCAAGGTACG GGAGCTGGAG 
GCTGAGCTTG ATGCAGAGCA GAAGAAGCAC GCCGAGGCCC TTAAGGGCGT GCGCAAGCAT 
GAGCGCCGTG TCAAGGAGCT CGCATACCAG GCCGAGGAGG ACAGGAAGAA CCTGGCTCGC 
ATGCAGGACC TGGTGGACAA GCTGCAGAGC AAGGTCAAGA GCTACAAGCG CCAGTTTGAG 
GAGGCGGAGC AGCAGGCCAA CACCAACCTG GCCAAGTATC GCAAGGCCCA GCACGAGCTG 
GATGATGCGG AGGAGCGGGC AGACATGGCG GAAACCCAGG CCAACAAGCT GCGGGCACGG 
ACCCGGGACG CCCTGGGCCC CAAGCTATCC CTTTCTCCTC AGCACAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527243.1
CDS	25..6018
Translation	MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGYQEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPMNPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVVAAYKGKRRSDSPPHIYAVADNAYNDMLRSKGRLDSSPTADPDLGPGRPLPRQPVHADHQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKVRRLRLSDVGGPHSYSFCSQPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKLSLSPQHKE

Target ORF information:

RefSeq Version	XM_011528941.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528941.2

ORF Insert Sequence:

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAGTAAG GGCCGCCTGG ACTCCTCCCC AACCGCAGAC 
CCCGACCTCG GTCCCGGGAG GCCTCTACCG AGACAACCAG TCCATGCTGA TCACCAATTT 
CTGGCAACAA AGACGGGGGG CACCCTTGAG GATCAAATCA TCGAGGCCAA CCCTGCCATG 
GAGGCCTTTG GCAACGCCAA GACCCTGAGG AATGATAACT CCTCCCGCTT TGGCAAGTTC 
ATCCGCATTC ACTTTGGTCC CTCTGGGAAG CTGGCATCCG CGGATATTGA CAGCTATCTC 
CTGGAGAAGT CGCGGGTGAT CTTCCAGTTG CCTGGTGAGC GCAGCTACCA TGTCTACTAC 
CAGATCCTCT CAGGGAGGAA GCCAGAGCTG CAGGACATGC TGCTTCTGTC TATGAACCCC 
TATGACTACC ACTTCTGCAG CCAGGGCGTC ATCACCGTGG ACAACATGAA TGATGGGGAG 
GAGCTCATCG CCACCGACCA TGCCATGGAC ATCCTAGGCT TCAGCGTGGA TGAGAAATGT 
GCCTGCTATA AGATCGTGGG CGCCCTCCTG CACTTTGGCA ACATGAAGTT CAAGCAGAAG 
CAGCGGGAGG AGCAGGCGGA GGCCGATGGC ACTGAGAGTG CTGACAAGGC TGCCTACCTG 
ATGGGGGTCA GCAGTGGGGA CCTCCTCAAA GGCCTTTTGC ACCCCCGGGT GCGTGTAGGG 
AACGAGTACG TGACCAAGGG CCAGAGTGTG GAGCAGGTGG TGTTTGCTGT GGGGGCTCTG 
GCCAAGGCCA CCTATGACCG GCTGTTCAGG TGGCTGGTGT CTCGGATCAA CCAGACCCTG 
GACACAAAGC TGCCCCGGCA GTTCTTCATC GGGGTTCTGG ACATCGCTGG GTTTGAGATC 
TTTGAGTTCA ACAGCTTCGA ACAGCTGTGC ATCAACTTCA CCAATGAGAA ATTGCAGCAG 
TTCTTCAACC AGCACATGTT TGTGCTGGAG CAGGAGGAGT ACAAGCGGGA GGGCATCGAC 
TGGGTCTTCA TCGACTTCGG CCTTGACCTG CAGCCTTGCA TCGACCTCAT CGAGAAGGTT 
AGAAGACTGA GGCTTAGTGA TGTCGGAGGG CCCCACAGCT ACTCGTTCTG TTCCCAGCCA 
CTGGGCATCC TGTCCATCCT GGAGGAGGAA TGCATGTTCC CCAAGGCCTC AGACGCCAGC 
TTCCGGGCCA AGCTCTACGA CAACCACGCG GGGAAGTCAC CCAATTTCCA GCAGCCTCGG 
CCTGACAAGA AGCGCAAGTA CCAGGCCCAC TTCGAGGTGG TCCACTACGC AGGCGTGGTG 
CCTTACAGCA TTGTGGGCTG GCTGGAGAAA AACAAGGATC CCCTGAATGA GACCGTGGTC 
CCCATCTTCC AGAAGTCACA GAATAGGCTC CTGGCGACTC TCTATGAGAA TTATGCGGGC 
TCCTGCTCCA CTGAGCCCCC CAAGTCTGGG GTGAAAGAGA AGCGTAAGAA GGCAGCATCG 
TTCCAGACGG TGTCCCAGCT GCACAAGGAG AACCTCAACA AGCTGATGAC CAACCTGCGG 
GCCACACAGC CCCACTTCGT CCGCTGCATT GTCCCCAACG AGAACAAAAC CCCAGGGGTC 
ATGGATGCCT TCTTGGTGCT ACACCAGCTG CGCTGCAATG GGGTCCTGGA GGGGATCCGG 
ATCTGCCGCC AAGGGTTCCC CAACAGGTTG CTCTACACCG ACTTCCGGCA GCGGTACCGT 
ATCCTGAACC CCAGTGCCAT CCCGGATGAC ACCTTCATGG ACAGCAGGAA GGCCACAGAG 
AAACTGCTGG GCTCGCTGGA CTTGGATCAC ACCCAGTACC AGTTTGGCCA CACCAAGGTG 
TTCTTCAAGG CTGGGCTTCT AGGCGTCCTG GAAGAGCTCC GTGACCAGCG CCTGGCCAAG 
GTGCTGACGC TGCTGCAGGC GCGGAGCCGT GGCCGCCTCA TGCGCCTTGA GTACCAGCGC 
CTGCTGGGAG GCAGGGATGC GCTGTTCACC ATCCAGTGGA ACATCCGTGC CTTCAATGCC 
GTCAAGAACT GGTCATGGAT GAAGCTCTTT TTCAAGATGA AGCCGCTGCT GCGCTCGGCG 
CAGGCTGAGG AGGAGCTGGC GGCCCTGCGG GCAGAGCTGC GGGGGTTGCG AGGGGCGCTG 
GCTGCGGCCG AGGCCAAGCG CCAGGAACTG GAGGAGACGC ACGTCAGCAT CACCCAGGAG 
AAGAATGACC TGGCCCTGCA GCTGCAGGCT GAGCAGGACA ACCTGGCAGA TGCCGAGGAG 
CGCTGCCACT TGCTGATCAA GTCCAAGGTG CAGCTGGAGG GGAAGGTGAA GGAGCTGAGT 
GAGCGGCTGG AGGATGAGGA GGAGGTGAAC GCTGACCTGG CCGCCCGCCG GCGCAAGCTG 
GAGGACGAGT GCACGGAGCT CAAGAAGGAC ATTGATGACC TGGAGCTGAC ACTGGCCAAA 
GCTGAGAAGG AGAAGCAAGC CACTGAGAAC AAGGTGAAGA ACCTGACGGA AGAGATGGCT 
GCGCTGGACG AGTCAGTGGC CCGGCTGACC AAGGAGAAGA AGGCGTTGCA GGAGGCCCAC 
CAACAGGCCC TGGGTGACCT GCAGGCCGAG GAGGACCGTG TGAGCGCGCT GACCAAGGCC 
AAGCTCCGGC TGGAGCAACA GGTGGAGGAC CTGGAATGCT CCCTGGAGCA GGAGAAGAAG 
CTGCGCATGG ACACGGAGCG GGCCAAGCGC AAGCTGGAGG GTGACCTGAA GCTGACGCAG 
GAGTCGGTGG CTGATGCTGC TCAAGACAAG CAGCAGCTGG AGGAGAAGCT CAAGAAGAAG 
GACTCCGAGC TGAGCCAGCT GAGCCTGCGG GTGGAAGACG AGCAGCTCTT GGGGGCCCAG 
ATGCAGAAGA AGATCAAGGA GCTGCAGGCT CGGGCGGAGG AGCTGGAAGA GGAGCTGGAG 
GCAGAGCGGG CAGCCCGGGC CCGCGTGGAG AAGCAGCGTG CAGAGGCGGC GCGGGAGCTG 
GAGGAGCTGA GCGAGCGGCT GGAGGAGGCA GGCGGCGCAT CCGCGGGGCA GCGCGAGGGC 
TGCCGCAAGC GGGAGGCGGA GCTGGGGAGG CTGCGGCGGG AGCTGGAGGA GGCGGCGCTG 
CGGCACGAGG CCACAGTGGC GGCACTGCGG CGCAAGCAGG CGGAGGGCGC GGCGGAGCTG 
GGGGAGCAGG TGGACAGCCT GCAGCGGGTG CGGCAGAAGC TGGAGAAGGA GAAGAGTGAG 
CTGCGCATGG AGGTGGACGA CCTGGCTGCC AACGTGGAGA CTCTGACCCG CGCCAAGGCC 
AGTGCAGAGA AGCTGTGCCG GACCTATGAG GATCAGCTAA GCGAGGCCAA GATCAAGGTG 
GAGGAGCTGC AGCGGCAGCT GGCGGACGCA AGCACGCAGC GTGGGCGACT ACAGACGGAA 
AGCGGGGAGC TGAGTCGCCT GCTAGAGGAG AAGGAGTGTC TGATCAGTCA GCTGAGCCGT 
GGAAAGGCCC TGGCCGCCCA AAGCCTGGAA GAGTTGCGGC GCCAGCTAGA GGAGGAAAGC 
AAGGCCAAGA GTGCCCTGGC CCACGCCGTG CAGGCTCTGC GGCACGACTG TGACCTCCTG 
CGGGAGCAAC ACGAGGAGGA GGCTGAGGCC CAGGCTGAGC TGCAGCGGCT GCTGTCCAAG 
GCCAATGCCG AGGTGGCCCA GTGGAGGAGC AAGTACGAAG CAGATGCCAT CCAGAGGACC 
GAGGAGCTGG AGGAGGCCAA AAAAAAGCTG GCACTGCGGC TGCAGGAGGC AGAGGAGGGC 
GTGGAGGCTG CCAACGCCAA GTGCTCATCG TTGGAGAAGG CCAAGCTGCG GCTACAGACA 
GAGTCAGAGG ATGTAACCCT GGAGCTGGAG CGGGCGACCT CAGCAGCTGC TGCGCTGGAC 
AAGAAGCAGC GGCACTTGGA ACGGGCACTG GAGGAACGGC GGCGGCAGGA GGAGGAGATG 
CAGCGGGAGC TGGAGGCGGC ACAGAGGGAG TCCCGTGGCC TGGGCACCGA GCTCTTCCGG 
CTGCGGCACG GCCACGAGGA GGCACTTGAA GCCCTGGAGA CGCTCAAGCG GGAGAACAAG 
AACCTGCAGG AGGAGATCAG CGACCTCACA GACCAGGTGA GTCTCAGTGG GAAGAGCATC 
CAGGAACTGG AGAAAACCAA GAAGGCGCTG GAAGGCGAGA AGAGTGAGAT CCAGGCTGCA 
CTGGAGGAGG CAGAGGGGGC CCTGGAGCTG GAGGAGACCA AGACGCTGCG GATCCAGCTG 
GAGCTCTCCC AGGTCAAAGC AGAAGTGGAC CGGAAGCTGG CAGAGAAAGA CGAGGAGTGC 
GCTAACCTGA GGCGCAACCA CCAGCGAGCT GTGGAGTCCC TGCAGGCCTC CCTGGATGCA 
GAGACACGGG CCCGCAATGA GGCGCTGCGG CTCAAGAAGA AGATGGAGGG TGACCTCAAC 
GACCTGGAGC TGCAGCTGGG CCATGCCACC CGTCAGGCCA CAGAGGCCCA GGCTGCCACG 
CGGCTGATGC AGGCACAGCT CAAGGAGGAG CAGGCAGGGC GGGACGAGGA GCAGCGGCTG 
GCAGCTGAGC TCCACGAGCA GGCGCAGGCT CTGGAGCGCC GGGCCTCGCT GCTGGCTGCG 
GAGCTGGAGG AGCTGCGGGC TGCCCTGGAG CAGGGCGAGC GCAGCCGGCG ACTGGCAGAG 
CAGGAGCTTT TGGAGGCCAC CGAGCGCCTC AACCTTCTGC ATTCGCAGAA CACAGGCCTC 
CTAAACCAGA AGAAGAAGCT GGAGGCGGAC TTGGCCCAGC TGAGCGGGGA GGTGGAGGAG 
GCTGCACAGG AGAGGCGGGA GGCTGAGGAG AAGGCCAAAA AGGCCATCAC TGATGCGGCC 
ATGATGGCCG AGGAGCTGAA GAAGGAGCAG GACACAAGTG CACACCTGGA ACGGATGAAG 
AAGACGCTGG AGCAGACGGT GCGCGAGCTC CAGGCCCGCC TTGAGGAGGC AGAACAGGCC 
GCCCTCCGTG GCGGGAAGAA GCAGGTGCAG AAGCTGGAGG CCAAGGTACG GGAGCTGGAG 
GCTGAGCTTG ATGCAGAGCA GAAGAAGCAC GCCGAGGCCC TTAAGGGCGT GCGCAAGCAT 
GAGCGCCGTG TCAAGGAGCT CGCATACCAG GCCGAGGAGG ACAGGAAGAA CCTGGCTCGC 
ATGCAGGACC TGGTGGACAA GCTGCAGAGC AAGGTCAAGA GCTACAAGCG CCAGTTTGAG 
GAGGCGGAGC AGCAGGCCAA CACCAACCTG GCCAAGTATC GCAAGGCCCA GCACGAGCTG 
GATGATGCGG AGGAGCGGGC AGACATGGCG GAAACCCAGG CCAACAAGCT GCGGGCACGG 
ACCCGGGACG CCCTGGGCCC CAAGCTATCC CTTTCTCCTC AGCACAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( XM_017027987.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883476.1 Homo sapiens myosin, heavy chain 7B, cardiac muscle, beta (MYH7B), transcript variant X4, mRNA.

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CloneID	OHu70760
Clone ID Related Accession (Same CDS sequence)	XM_017027987.1
Accession Version	XM_017027987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myosin-7B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCA GTCTATACGG CCTCCGTAGT GGCTGCTTAC AAGGGAAAGC GCCGCTCAGA 
TTCCCCGCCC CATATATATG CGGTGGCGGA CAACGCCTAC AACGACATGC TGCGCAACCG 
AGACAACCAG TCCATGCTGA TCACCAATTT CTGGCAACAA AGACGGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCCAA CCCTGCCATG GAGGCCTTTG GCAACGCCAA GACCCTGAGG 
AATGATAACT CCTCCCGCTT TGGCAAGTTC ATCCGCATTC ACTTTGGTCC CTCTGGGAAG 
CTGGCATCCG CGGATATTGA CAGCTATCTC CTGGAGAAGT CGCGGGTGAT CTTCCAGTTG 
CCTGGTGAGC GCAGCTACCA TGTCTACTAC CAGATCCTCT CAGGGAGGAA GCCAGAGCTG 
CAGGACATGC TGCTTCTGTC TATGAACCCC TATGACTACC ACTTCTGCAG CCAGGGCGTC 
ATCACCGTGG ACAACATGAA TGATGGGGAG GAGCTCATCG CCACCGACCA TGCCATGGAC 
ATCCTAGGCT TCAGCGTGGA TGAGAAATGT GCCTGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCGGA GGCCGATGGC 
ACTGAGAGTG CTGACAAGGC TGCCTACCTG ATGGGGGTCA GCAGTGGGGA CCTCCTCAAA 
GGCCTTTTGC ACCCCCGGGT GCGTGTAGGG AACGAGTACG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TGTTTGCTGT GGGGGCTCTG GCCAAGGCCA CCTATGACCG GCTGTTCAGG 
TGGCTGGTGT CTCGGATCAA CCAGACCCTG GACACAAAGC TGCCCCGGCA GTTCTTCATC 
GGGGTTCTGG ACATCGCTGG GTTTGAGATC TTTGAGTTCA ACAGCTTCGA ACAGCTGTGC 
ATCAACTTCA CCAATGAGAA ATTGCAGCAG TTCTTCAACC AGCACATGTT TGTGCTGGAG 
CAGGAGGAGT ACAAGCGGGA GGGCATCGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCTTGCA TCGACCTCAT CGAGAAGGTT AGAAGACTGA GGCTTAGTGA TGTCGGAGGG 
CCCCACAGCT ACTCGTTCTG TTCCCAGCCA CTGGGCATCC TGTCCATCCT GGAGGAGGAA 
TGCATGTTCC CCAAGGCCTC AGACGCCAGC TTCCGGGCCA AGCTCTACGA CAACCACGCG 
GGGAAGTCAC CCAATTTCCA GCAGCCTCGG CCTGACAAGA AGCGCAAGTA CCAGGCCCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTG CCTTACAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGATC CCCTGAATGA GACCGTGGTC CCCATCTTCC AGAAGTCACA GAATAGGCTC 
CTGGCGACTC TCTATGAGAA TTATGCGGGC TCCTGCTCCA CTGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AGCGTAAGAA GGCAGCATCG TTCCAGACGG TGTCCCAGCT GCACAAGGAG 
AACCTCAACA AGCTGATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGCTGCATT 
GTCCCCAACG AGAACAAAAC CCCAGGGGTC ATGGATGCCT TCTTGGTGCT ACACCAGCTG 
CGCTGCAATG GGGTCCTGGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGGTTG 
CTCTACACCG ACTTCCGGCA GCGGTACCGT ATCCTGAACC CCAGTGCCAT CCCGGATGAC 
ACCTTCATGG ACAGCAGGAA GGCCACAGAG AAACTGCTGG GCTCGCTGGA CTTGGATCAC 
ACCCAGTACC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCGTCCTG 
GAAGAGCTCC GTGACCAGCG CCTGGCCAAG GTGCTGACGC TGCTGCAGGC GCGGAGCCGT 
GGCCGCCTCA TGCGCCTTGA GTACCAGCGC CTGCTGGGAG GCAGGGATGC GCTGTTCACC 
ATCCAGTGGA ACATCCGTGC CTTCAATGCC GTCAAGAACT GGTCATGGAT GAAGCTCTTT 
TTCAAGATGA AGCCGCTGCT GCGCTCGGCG CAGGCTGAGG AGGAGCTGGC GGCCCTGCGG 
GCAGAGCTGC GGGGGTTGCG AGGGGCGCTG GCTGCGGCCG AGGCCAAGCG CCAGGAACTG 
GAGGAGACGC ACGTCAGCAT CACCCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCT 
GAGCAGGACA ACCTGGCAGA TGCCGAGGAG CGCTGCCACT TGCTGATCAA GTCCAAGGTG 
CAGCTGGAGG GGAAGGTGAA GGAGCTGAGT GAGCGGCTGG AGGATGAGGA GGAGGTGAAC 
GCTGACCTGG CCGCCCGCCG GCGCAAGCTG GAGGACGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC ACTGGCCAAA GCTGAGAAGG AGAAGCAAGC CACTGAGAAC 
AAGGTGAAGA ACCTGACGGA AGAGATGGCT GCGCTGGACG AGTCAGTGGC CCGGCTGACC 
AAGGAGAAGA AGGCGTTGCA GGAGGCCCAC CAACAGGCCC TGGGTGACCT GCAGGCCGAG 
GAGGACCGTG TGAGCGCGCT GACCAAGGCC AAGCTCCGGC TGGAGCAACA GGTGGAGGAC 
CTGGAATGCT CCCTGGAGCA GGAGAAGAAG CTGCGCATGG ACACGGAGCG GGCCAAGCGC 
AAGCTGGAGG GTGACCTGAA GCTGACGCAG GAGTCGGTGG CTGATGCTGC TCAAGACAAG 
CAGCAGCTGG AGGAGAAGCT CAAGAAGAAG GACTCCGAGC TGAGCCAGCT GAGCCTGCGG 
GTGGAAGACG AGCAGCTCTT GGGGGCCCAG ATGCAGAAGA AGATCAAGGA GCTGCAGGCT 
CGGGCGGAGG AGCTGGAAGA GGAGCTGGAG GCAGAGCGGG CAGCCCGGGC CCGCGTGGAG 
AAGCAGCGTG CAGAGGCGGC GCGGGAGCTG GAGGAGCTGA GCGAGCGGCT GGAGGAGGCA 
GGCGGCGCAT CCGCGGGGCA GCGCGAGGGC TGCCGCAAGC GGGAGGCGGA GCTGGGGAGG 
CTGCGGCGGG AGCTGGAGGA GGCGGCGCTG CGGCACGAGG CCACAGTGGC GGCACTGCGG 
CGCAAGCAGG CGGAGGGCGC GGCGGAGCTG GGGGAGCAGG TGGACAGCCT GCAGCGGGTG 
CGGCAGAAGC TGGAGAAGGA GAAGAGTGAG CTGCGCATGG AGGTGGACGA CCTGGCTGCC 
AACGTGGAGA CTCTGACCCG CGCCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GATCAGCTAA GCGAGGCCAA GATCAAGGTG GAGGAGCTGC AGCGGCAGCT GGCGGACGCA 
AGCACGCAGC GTGGGCGACT ACAGACGGAA AGCGGGGAGC TGAGTCGCCT GCTAGAGGAG 
AAGGAGTGTC TGATCAGTCA GCTGAGCCGT GGAAAGGCCC TGGCCGCCCA AAGCCTGGAA 
GAGTTGCGGC GCCAGCTAGA GGAGGAAAGC AAGGCCAAGA GTGCCCTGGC CCACGCCGTG 
CAGGCTCTGC GGCACGACTG TGACCTCCTG CGGGAGCAAC ACGAGGAGGA GGCTGAGGCC 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAG GCCAATGCCG AGGTGGCCCA GTGGAGGAGC 
AAGTACGAAG CAGATGCCAT CCAGAGGACC GAGGAGCTGG AGGAGGCCAA AAAAAAGCTG 
GCACTGCGGC TGCAGGAGGC AGAGGAGGGC GTGGAGGCTG CCAACGCCAA GTGCTCATCG 
TTGGAGAAGG CCAAGCTGCG GCTACAGACA GAGTCAGAGG ATGTAACCCT GGAGCTGGAG 
CGGGCGACCT CAGCAGCTGC TGCGCTGGAC AAGAAGCAGC GGCACTTGGA ACGGGCACTG 
GAGGAACGGC GGCGGCAGGA GGAGGAGATG CAGCGGGAGC TGGAGGCGGC ACAGAGGGAG 
TCCCGTGGCC TGGGCACCGA GCTCTTCCGG CTGCGGCACG GCCACGAGGA GGCACTTGAA 
GCCCTGGAGA CGCTCAAGCG GGAGAACAAG AACCTGCAGG AGGAGATCAG CGACCTCACA 
GACCAGGTGA GTCTCAGTGG GAAGAGCATC CAGGAACTGG AGAAAACCAA GAAGGCGCTG 
GAAGGCGAGA AGAGTGAGAT CCAGGCTGCA CTGGAGGAGG CAGAGGGGGC CCTGGAGCTG 
GAGGAGACCA AGACGCTGCG GATCCAGCTG GAGCTCTCCC AGGTCAAAGC AGAAGTGGAC 
CGGAAGCTGG CAGAGAAAGA CGAGGAGTGC GCTAACCTGA GGCGCAACCA CCAGCGAGCT 
GTGGAGTCCC TGCAGGCCTC CCTGGATGCA GAGACACGGG CCCGCAATGA GGCGCTGCGG 
CTCAAGAAGA AGATGGAGGG TGACCTCAAC GACCTGGAGC TGCAGCTGGG CCATGCCACC 
CGTCAGGCCA CAGAGGCCCA GGCTGCCACG CGGCTGATGC AGGCACAGCT CAAGGAGGAG 
CAGGCAGGGC GGGACGAGGA GCAGCGGCTG GCAGCTGAGC TCCACGAGCA GGCGCAGGCT 
CTGGAGCGCC GGGCCTCGCT GCTGGCTGCG GAGCTGGAGG AGCTGCGGGC TGCCCTGGAG 
CAGGGCGAGC GCAGCCGGCG ACTGGCAGAG CAGGAGCTTT TGGAGGCCAC CGAGCGCCTC 
AACCTTCTGC ATTCGCAGAA CACAGGCCTC CTAAACCAGA AGAAGAAGCT GGAGGCGGAC 
TTGGCCCAGC TGAGCGGGGA GGTGGAGGAG GCTGCACAGG AGAGGCGGGA GGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC TGATGCGGCC ATGATGGCCG AGGAGCTGAA GAAGGAGCAG 
GACACAAGTG CACACCTGGA ACGGATGAAG AAGACGCTGG AGCAGACGGT GCGCGAGCTC 
CAGGCCCGCC TTGAGGAGGC AGAACAGGCC GCCCTCCGTG GCGGGAAGAA GCAGGTGCAG 
AAGCTGGAGG CCAAGGTACG GGAGCTGGAG GCTGAGCTTG ATGCAGAGCA GAAGAAGCAC 
GCCGAGGCCC TTAAGGGCGT GCGCAAGCAT GAGCGCCGTG TCAAGGAGCT CGCATACCAG 
GCCGAGGAGG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TGGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG CCAGTTTGAG GAGGCGGAGC AGCAGGCCAA CACCAACCTG 
GCCAAGTATC GCAAGGCCCA GCACGAGCTG GATGATGCGG AGGAGCGGGC AGACATGGCG 
GAAACCCAGG CCAACAAGCT GCGGGCACGG ACCCGGGACG CCCTGGGCCC CAAGCTATCC 
CTTTCTCCTC AGCACAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883476.1
CDS	146..5569
Translation	MAPSLYGLRSGCLQGKAPLRFPAPYICGGGQRLQRHAAQPRQPVHADHQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKVRRLRLSDVGGPHSYSFCSQPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKLSLSPQHKE

Target ORF information:

RefSeq Version	XM_017027987.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin, heavy chain 7B, cardiac muscle, beta (MYH7B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027987.1

ORF Insert Sequence:

ATGGCTCCCA GTCTATACGG CCTCCGTAGT GGCTGCTTAC AAGGGAAAGC GCCGCTCAGA 
TTCCCCGCCC CATATATATG CGGTGGCGGA CAACGCCTAC AACGACATGC TGCGCAACCG 
AGACAACCAG TCCATGCTGA TCACCAATTT CTGGCAACAA AGACGGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCCAA CCCTGCCATG GAGGCCTTTG GCAACGCCAA GACCCTGAGG 
AATGATAACT CCTCCCGCTT TGGCAAGTTC ATCCGCATTC ACTTTGGTCC CTCTGGGAAG 
CTGGCATCCG CGGATATTGA CAGCTATCTC CTGGAGAAGT CGCGGGTGAT CTTCCAGTTG 
CCTGGTGAGC GCAGCTACCA TGTCTACTAC CAGATCCTCT CAGGGAGGAA GCCAGAGCTG 
CAGGACATGC TGCTTCTGTC TATGAACCCC TATGACTACC ACTTCTGCAG CCAGGGCGTC 
ATCACCGTGG ACAACATGAA TGATGGGGAG GAGCTCATCG CCACCGACCA TGCCATGGAC 
ATCCTAGGCT TCAGCGTGGA TGAGAAATGT GCCTGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCGGA GGCCGATGGC 
ACTGAGAGTG CTGACAAGGC TGCCTACCTG ATGGGGGTCA GCAGTGGGGA CCTCCTCAAA 
GGCCTTTTGC ACCCCCGGGT GCGTGTAGGG AACGAGTACG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TGTTTGCTGT GGGGGCTCTG GCCAAGGCCA CCTATGACCG GCTGTTCAGG 
TGGCTGGTGT CTCGGATCAA CCAGACCCTG GACACAAAGC TGCCCCGGCA GTTCTTCATC 
GGGGTTCTGG ACATCGCTGG GTTTGAGATC TTTGAGTTCA ACAGCTTCGA ACAGCTGTGC 
ATCAACTTCA CCAATGAGAA ATTGCAGCAG TTCTTCAACC AGCACATGTT TGTGCTGGAG 
CAGGAGGAGT ACAAGCGGGA GGGCATCGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCTTGCA TCGACCTCAT CGAGAAGGTT AGAAGACTGA GGCTTAGTGA TGTCGGAGGG 
CCCCACAGCT ACTCGTTCTG TTCCCAGCCA CTGGGCATCC TGTCCATCCT GGAGGAGGAA 
TGCATGTTCC CCAAGGCCTC AGACGCCAGC TTCCGGGCCA AGCTCTACGA CAACCACGCG 
GGGAAGTCAC CCAATTTCCA GCAGCCTCGG CCTGACAAGA AGCGCAAGTA CCAGGCCCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTG CCTTACAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGATC CCCTGAATGA GACCGTGGTC CCCATCTTCC AGAAGTCACA GAATAGGCTC 
CTGGCGACTC TCTATGAGAA TTATGCGGGC TCCTGCTCCA CTGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AGCGTAAGAA GGCAGCATCG TTCCAGACGG TGTCCCAGCT GCACAAGGAG 
AACCTCAACA AGCTGATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGCTGCATT 
GTCCCCAACG AGAACAAAAC CCCAGGGGTC ATGGATGCCT TCTTGGTGCT ACACCAGCTG 
CGCTGCAATG GGGTCCTGGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGGTTG 
CTCTACACCG ACTTCCGGCA GCGGTACCGT ATCCTGAACC CCAGTGCCAT CCCGGATGAC 
ACCTTCATGG ACAGCAGGAA GGCCACAGAG AAACTGCTGG GCTCGCTGGA CTTGGATCAC 
ACCCAGTACC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCGTCCTG 
GAAGAGCTCC GTGACCAGCG CCTGGCCAAG GTGCTGACGC TGCTGCAGGC GCGGAGCCGT 
GGCCGCCTCA TGCGCCTTGA GTACCAGCGC CTGCTGGGAG GCAGGGATGC GCTGTTCACC 
ATCCAGTGGA ACATCCGTGC CTTCAATGCC GTCAAGAACT GGTCATGGAT GAAGCTCTTT 
TTCAAGATGA AGCCGCTGCT GCGCTCGGCG CAGGCTGAGG AGGAGCTGGC GGCCCTGCGG 
GCAGAGCTGC GGGGGTTGCG AGGGGCGCTG GCTGCGGCCG AGGCCAAGCG CCAGGAACTG 
GAGGAGACGC ACGTCAGCAT CACCCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCT 
GAGCAGGACA ACCTGGCAGA TGCCGAGGAG CGCTGCCACT TGCTGATCAA GTCCAAGGTG 
CAGCTGGAGG GGAAGGTGAA GGAGCTGAGT GAGCGGCTGG AGGATGAGGA GGAGGTGAAC 
GCTGACCTGG CCGCCCGCCG GCGCAAGCTG GAGGACGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC ACTGGCCAAA GCTGAGAAGG AGAAGCAAGC CACTGAGAAC 
AAGGTGAAGA ACCTGACGGA AGAGATGGCT GCGCTGGACG AGTCAGTGGC CCGGCTGACC 
AAGGAGAAGA AGGCGTTGCA GGAGGCCCAC CAACAGGCCC TGGGTGACCT GCAGGCCGAG 
GAGGACCGTG TGAGCGCGCT GACCAAGGCC AAGCTCCGGC TGGAGCAACA GGTGGAGGAC 
CTGGAATGCT CCCTGGAGCA GGAGAAGAAG CTGCGCATGG ACACGGAGCG GGCCAAGCGC 
AAGCTGGAGG GTGACCTGAA GCTGACGCAG GAGTCGGTGG CTGATGCTGC TCAAGACAAG 
CAGCAGCTGG AGGAGAAGCT CAAGAAGAAG GACTCCGAGC TGAGCCAGCT GAGCCTGCGG 
GTGGAAGACG AGCAGCTCTT GGGGGCCCAG ATGCAGAAGA AGATCAAGGA GCTGCAGGCT 
CGGGCGGAGG AGCTGGAAGA GGAGCTGGAG GCAGAGCGGG CAGCCCGGGC CCGCGTGGAG 
AAGCAGCGTG CAGAGGCGGC GCGGGAGCTG GAGGAGCTGA GCGAGCGGCT GGAGGAGGCA 
GGCGGCGCAT CCGCGGGGCA GCGCGAGGGC TGCCGCAAGC GGGAGGCGGA GCTGGGGAGG 
CTGCGGCGGG AGCTGGAGGA GGCGGCGCTG CGGCACGAGG CCACAGTGGC GGCACTGCGG 
CGCAAGCAGG CGGAGGGCGC GGCGGAGCTG GGGGAGCAGG TGGACAGCCT GCAGCGGGTG 
CGGCAGAAGC TGGAGAAGGA GAAGAGTGAG CTGCGCATGG AGGTGGACGA CCTGGCTGCC 
AACGTGGAGA CTCTGACCCG CGCCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GATCAGCTAA GCGAGGCCAA GATCAAGGTG GAGGAGCTGC AGCGGCAGCT GGCGGACGCA 
AGCACGCAGC GTGGGCGACT ACAGACGGAA AGCGGGGAGC TGAGTCGCCT GCTAGAGGAG 
AAGGAGTGTC TGATCAGTCA GCTGAGCCGT GGAAAGGCCC TGGCCGCCCA AAGCCTGGAA 
GAGTTGCGGC GCCAGCTAGA GGAGGAAAGC AAGGCCAAGA GTGCCCTGGC CCACGCCGTG 
CAGGCTCTGC GGCACGACTG TGACCTCCTG CGGGAGCAAC ACGAGGAGGA GGCTGAGGCC 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAG GCCAATGCCG AGGTGGCCCA GTGGAGGAGC 
AAGTACGAAG CAGATGCCAT CCAGAGGACC GAGGAGCTGG AGGAGGCCAA AAAAAAGCTG 
GCACTGCGGC TGCAGGAGGC AGAGGAGGGC GTGGAGGCTG CCAACGCCAA GTGCTCATCG 
TTGGAGAAGG CCAAGCTGCG GCTACAGACA GAGTCAGAGG ATGTAACCCT GGAGCTGGAG 
CGGGCGACCT CAGCAGCTGC TGCGCTGGAC AAGAAGCAGC GGCACTTGGA ACGGGCACTG 
GAGGAACGGC GGCGGCAGGA GGAGGAGATG CAGCGGGAGC TGGAGGCGGC ACAGAGGGAG 
TCCCGTGGCC TGGGCACCGA GCTCTTCCGG CTGCGGCACG GCCACGAGGA GGCACTTGAA 
GCCCTGGAGA CGCTCAAGCG GGAGAACAAG AACCTGCAGG AGGAGATCAG CGACCTCACA 
GACCAGGTGA GTCTCAGTGG GAAGAGCATC CAGGAACTGG AGAAAACCAA GAAGGCGCTG 
GAAGGCGAGA AGAGTGAGAT CCAGGCTGCA CTGGAGGAGG CAGAGGGGGC CCTGGAGCTG 
GAGGAGACCA AGACGCTGCG GATCCAGCTG GAGCTCTCCC AGGTCAAAGC AGAAGTGGAC 
CGGAAGCTGG CAGAGAAAGA CGAGGAGTGC GCTAACCTGA GGCGCAACCA CCAGCGAGCT 
GTGGAGTCCC TGCAGGCCTC CCTGGATGCA GAGACACGGG CCCGCAATGA GGCGCTGCGG 
CTCAAGAAGA AGATGGAGGG TGACCTCAAC GACCTGGAGC TGCAGCTGGG CCATGCCACC 
CGTCAGGCCA CAGAGGCCCA GGCTGCCACG CGGCTGATGC AGGCACAGCT CAAGGAGGAG 
CAGGCAGGGC GGGACGAGGA GCAGCGGCTG GCAGCTGAGC TCCACGAGCA GGCGCAGGCT 
CTGGAGCGCC GGGCCTCGCT GCTGGCTGCG GAGCTGGAGG AGCTGCGGGC TGCCCTGGAG 
CAGGGCGAGC GCAGCCGGCG ACTGGCAGAG CAGGAGCTTT TGGAGGCCAC CGAGCGCCTC 
AACCTTCTGC ATTCGCAGAA CACAGGCCTC CTAAACCAGA AGAAGAAGCT GGAGGCGGAC 
TTGGCCCAGC TGAGCGGGGA GGTGGAGGAG GCTGCACAGG AGAGGCGGGA GGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC TGATGCGGCC ATGATGGCCG AGGAGCTGAA GAAGGAGCAG 
GACACAAGTG CACACCTGGA ACGGATGAAG AAGACGCTGG AGCAGACGGT GCGCGAGCTC 
CAGGCCCGCC TTGAGGAGGC AGAACAGGCC GCCCTCCGTG GCGGGAAGAA GCAGGTGCAG 
AAGCTGGAGG CCAAGGTACG GGAGCTGGAG GCTGAGCTTG ATGCAGAGCA GAAGAAGCAC 
GCCGAGGCCC TTAAGGGCGT GCGCAAGCAT GAGCGCCGTG TCAAGGAGCT CGCATACCAG 
GCCGAGGAGG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TGGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG CCAGTTTGAG GAGGCGGAGC AGCAGGCCAA CACCAACCTG 
GCCAAGTATC GCAAGGCCCA GCACGAGCTG GATGATGCGG AGGAGCGGGC AGACATGGCG 
GAAACCCAGG CCAACAAGCT GCGGGCACGG ACCCGGGACG CCCTGGGCCC CAAGCTATCC 
CTTTCTCCTC AGCACAAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( XM_011528948.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527250.1 Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X5, mRNA.

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CloneID	OHu70762
Clone ID Related Accession (Same CDS sequence)	XM_011528948.2 , XM_011528947.2
Accession Version	XM_011528948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-7B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528948.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCT TTGGCAACGC CAAGACCCTG AGGAATGATA ACTCCTCCCG CTTTGGCAAG 
TTCATCCGCA TTCACTTTGG TCCCTCTGGG AAGCTGGCAT CCGCGGATAT TGACAGCTAT 
CTCCTGGAGA AGTCGCGGGT GATCTTCCAG TTGCCTGGTG AGCGCAGCTA CCATGTCTAC 
TACCAGATCC TCTCAGGGAG GAAGCCAGAG CTGCAGGACA TGCTGCTTCT GTCTATGAAC 
CCCTATGACT ACCACTTCTG CAGCCAGGGC GTCATCACCG TGGACAACAT GAATGATGGG 
GAGGAGCTCA TCGCCACCGA CCATGCCATG GACATCCTAG GCTTCAGCGT GGATGAGAAA 
TGTGCCTGCT ATAAGATCGT GGGCGCCCTC CTGCACTTTG GCAACATGAA GTTCAAGCAG 
AAGCAGCGGG AGGAGCAGGC GGAGGCCGAT GGCACTGAGA GTGCTGACAA GGCTGCCTAC 
CTGATGGGGG TCAGCAGTGG GGACCTCCTC AAAGGCCTTT TGCACCCCCG GGTGCGTGTA 
GGGAACGAGT ACGTGACCAA GGGCCAGAGT GTGGAGCAGG TGGTGTTTGC TGTGGGGGCT 
CTGGCCAAGG CCACCTATGA CCGGCTGTTC AGGTGGCTGG TGTCTCGGAT CAACCAGACC 
CTGGACACAA AGCTGCCCCG GCAGTTCTTC ATCGGGGTTC TGGACATCGC TGGGTTTGAG 
ATCTTTGAGT TCAACAGCTT CGAACAGCTG TGCATCAACT TCACCAATGA GAAATTGCAG 
CAGTTCTTCA ACCAGCACAT GTTTGTGCTG GAGCAGGAGG AGTACAAGCG GGAGGGCATC 
GACTGGGTCT TCATCGACTT CGGCCTTGAC CTGCAGCCTT GCATCGACCT CATCGAGAAG 
GTTAGAAGAC TGAGGCTTAG TGATGTCGGA GGGCCCCACA GCTACTCGTT CTGTTCCCAG 
CCACTGGGCA TCCTGTCCAT CCTGGAGGAG GAATGCATGT TCCCCAAGGC CTCAGACGCC 
AGCTTCCGGG CCAAGCTCTA CGACAACCAC GCGGGGAAGT CACCCAATTT CCAGCAGCCT 
CGGCCTGACA AGAAGCGCAA GTACCAGGCC CACTTCGAGG TGGTCCACTA CGCAGGCGTG 
GTGCCTTACA GCATTGTGGG CTGGCTGGAG AAAAACAAGG ATCCCCTGAA TGAGACCGTG 
GTCCCCATCT TCCAGAAGTC ACAGAATAGG CTCCTGGCGA CTCTCTATGA GAATTATGCG 
GGCTCCTGCT CCACTGAGCC CCCCAAGTCT GGGGTGAAAG AGAAGCGTAA GAAGGCAGCA 
TCGTTCCAGA CGGTGTCCCA GCTGCACAAG GAGAACCTCA ACAAGCTGAT GACCAACCTG 
CGGGCCACAC AGCCCCACTT CGTCCGCTGC ATTGTCCCCA ACGAGAACAA AACCCCAGGG 
GTCATGGATG CCTTCTTGGT GCTACACCAG CTGCGCTGCA ATGGGGTCCT GGAGGGGATC 
CGGATCTGCC GCCAAGGGTT CCCCAACAGG TTGCTCTACA CCGACTTCCG GCAGCGGTAC 
CGTATCCTGA ACCCCAGTGC CATCCCGGAT GACACCTTCA TGGACAGCAG GAAGGCCACA 
GAGAAACTGC TGGGCTCGCT GGACTTGGAT CACACCCAGT ACCAGTTTGG CCACACCAAG 
GTGTTCTTCA AGGCTGGGCT TCTAGGCGTC CTGGAAGAGC TCCGTGACCA GCGCCTGGCC 
AAGGTGCTGA CGCTGCTGCA GGCGCGGAGC CGTGGCCGCC TCATGCGCCT TGAGTACCAG 
CGCCTGCTGG GAGGCAGGGA TGCGCTGTTC ACCATCCAGT GGAACATCCG TGCCTTCAAT 
GCCGTCAAGA ACTGGTCATG GATGAAGCTC TTTTTCAAGA TGAAGCCGCT GCTGCGCTCG 
GCGCAGGCTG AGGAGGAGCT GGCGGCCCTG CGGGCAGAGC TGCGGGGGTT GCGAGGGGCG 
CTGGCTGCGG CCGAGGCCAA GCGCCAGGAA CTGGAGGAGA CGCACGTCAG CATCACCCAG 
GAGAAGAATG ACCTGGCCCT GCAGCTGCAG GCTGAGCAGG ACAACCTGGC AGATGCCGAG 
GAGCGCTGCC ACTTGCTGAT CAAGTCCAAG GTGCAGCTGG AGGGGAAGGT GAAGGAGCTG 
AGTGAGCGGC TGGAGGATGA GGAGGAGGTG AACGCTGACC TGGCCGCCCG CCGGCGCAAG 
CTGGAGGACG AGTGCACGGA GCTCAAGAAG GACATTGATG ACCTGGAGCT GACACTGGCC 
AAAGCTGAGA AGGAGAAGCA AGCCACTGAG AACAAGGTGA AGAACCTGAC GGAAGAGATG 
GCTGCGCTGG ACGAGTCAGT GGCCCGGCTG ACCAAGGAGA AGAAGGCGTT GCAGGAGGCC 
CACCAACAGG CCCTGGGTGA CCTGCAGGCC GAGGAGGACC GTGTGAGCGC GCTGACCAAG 
GCCAAGCTCC GGCTGGAGCA ACAGGTGGAG GACCTGGAAT GCTCCCTGGA GCAGGAGAAG 
AAGCTGCGCA TGGACACGGA GCGGGCCAAG CGCAAGCTGG AGGGTGACCT GAAGCTGACG 
CAGGAGTCGG TGGCTGATGC TGCTCAAGAC AAGCAGCAGC TGGAGGAGAA GCTCAAGAAG 
AAGGACTCCG AGCTGAGCCA GCTGAGCCTG CGGGTGGAAG ACGAGCAGCT CTTGGGGGCC 
CAGATGCAGA AGAAGATCAA GGAGCTGCAG GCTCGGGCGG AGGAGCTGGA AGAGGAGCTG 
GAGGCAGAGC GGGCAGCCCG GGCCCGCGTG GAGAAGCAGC GTGCAGAGGC GGCGCGGGAG 
CTGGAGGAGC TGAGCGAGCG GCTGGAGGAG GCAGGCGGCG CATCCGCGGG GCAGCGCGAG 
GGCTGCCGCA AGCGGGAGGC GGAGCTGGGG AGGCTGCGGC GGGAGCTGGA GGAGGCGGCG 
CTGCGGCACG AGGCCACAGT GGCGGCACTG CGGCGCAAGC AGGCGGAGGG CGCGGCGGAG 
CTGGGGGAGC AGGTGGACAG CCTGCAGCGG GTGCGGCAGA AGCTGGAGAA GGAGAAGAGT 
GAGCTGCGCA TGGAGGTGGA CGACCTGGCT GCCAACGTGG AGACTCTGAC CCGCGCCAAG 
GCCAGTGCAG AGAAGCTGTG CCGGACCTAT GAGGATCAGC TAAGCGAGGC CAAGATCAAG 
GTGGAGGAGC TGCAGCGGCA GCTGGCGGAC GCAAGCACGC AGCGTGGGCG ACTACAGACG 
GAAAGCGGGG AGCTGAGTCG CCTGCTAGAG GAGAAGGAGT GTCTGATCAG TCAGCTGAGC 
CGTGGAAAGG CCCTGGCCGC CCAAAGCCTG GAAGAGTTGC GGCGCCAGCT AGAGGAGGAA 
AGCAAGGCCA AGAGTGCCCT GGCCCACGCC GTGCAGGCTC TGCGGCACGA CTGTGACCTC 
CTGCGGGAGC AACACGAGGA GGAGGCTGAG GCCCAGGCTG AGCTGCAGCG GCTGCTGTCC 
AAGGCCAATG CCGAGGTGGC CCAGTGGAGG AGCAAGTACG AAGCAGATGC CATCCAGAGG 
ACCGAGGAGC TGGAGGAGGC CAAAAAAAAG CTGGCACTGC GGCTGCAGGA GGCAGAGGAG 
GGCGTGGAGG CTGCCAACGC CAAGTGCTCA TCGTTGGAGA AGGCCAAGCT GCGGCTACAG 
ACAGAGTCAG AGGATGTAAC CCTGGAGCTG GAGCGGGCGA CCTCAGCAGC TGCTGCGCTG 
GACAAGAAGC AGCGGCACTT GGAACGGGCA CTGGAGGAAC GGCGGCGGCA GGAGGAGGAG 
ATGCAGCGGG AGCTGGAGGC GGCACAGAGG GAGTCCCGTG GCCTGGGCAC CGAGCTCTTC 
CGGCTGCGGC ACGGCCACGA GGAGGCACTT GAAGCCCTGG AGACGCTCAA GCGGGAGAAC 
AAGAACCTGC AGGAGGAGAT CAGCGACCTC ACAGACCAGG TGAGTCTCAG TGGGAAGAGC 
ATCCAGGAAC TGGAGAAAAC CAAGAAGGCG CTGGAAGGCG AGAAGAGTGA GATCCAGGCT 
GCACTGGAGG AGGCAGAGGG GGCCCTGGAG CTGGAGGAGA CCAAGACGCT GCGGATCCAG 
CTGGAGCTCT CCCAGGTCAA AGCAGAAGTG GACCGGAAGC TGGCAGAGAA AGACGAGGAG 
TGCGCTAACC TGAGGCGCAA CCACCAGCGA GCTGTGGAGT CCCTGCAGGC CTCCCTGGAT 
GCAGAGACAC GGGCCCGCAA TGAGGCGCTG CGGCTCAAGA AGAAGATGGA GGGTGACCTC 
AACGACCTGG AGCTGCAGCT GGGCCATGCC ACCCGTCAGG CCACAGAGGC CCAGGCTGCC 
ACGCGGCTGA TGCAGGCACA GCTCAAGGAG GAGCAGGCAG GGCGGGACGA GGAGCAGCGG 
CTGGCAGCTG AGCTCCACGA GCAGGCGCAG GCTCTGGAGC GCCGGGCCTC GCTGCTGGCT 
GCGGAGCTGG AGGAGCTGCG GGCTGCCCTG GAGCAGGGCG AGCGCAGCCG GCGACTGGCA 
GAGCAGGAGC TTTTGGAGGC CACCGAGCGC CTCAACCTTC TGCATTCGCA GAACACAGGC 
CTCCTAAACC AGAAGAAGAA GCTGGAGGCG GACTTGGCCC AGCTGAGCGG GGAGGTGGAG 
GAGGCTGCAC AGGAGAGGCG GGAGGCTGAG GAGAAGGCCA AAAAGGCCAT CACTGATGCG 
GCCATGATGG CCGAGGAGCT GAAGAAGGAG CAGGACACAA GTGCACACCT GGAACGGATG 
AAGAAGACGC TGGAGCAGAC GGTGCGCGAG CTCCAGGCCC GCCTTGAGGA GGCAGAACAG 
GCCGCCCTCC GTGGCGGGAA GAAGCAGGTG CAGAAGCTGG AGGCCAAGGT ACGGGAGCTG 
GAGGCTGAGC TTGATGCAGA GCAGAAGAAG CACGCCGAGG CCCTTAAGGG CGTGCGCAAG 
CATGAGCGCC GTGTCAAGGA GCTCGCATAC CAGGCCGAGG AGGACAGGAA GAACCTGGCT 
CGCATGCAGG ACCTGGTGGA CAAGCTGCAG AGCAAGGTCA AGAGCTACAA GCGCCAGTTT 
GAGGAGGCGG AGCAGCAGGC CAACACCAAC CTGGCCAAGT ATCGCAAGGC CCAGCACGAG 
CTGGATGATG CGGAGGAGCG GGCAGACATG GCGGAAACCC AGGCCAACAA GCTGCGGGCA 
CGGACCCGGG ACGCCCTGGG CCCCAAGCTA TCCCTTTCTC CTCAGCACAA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527250.1
CDS	334..5550
Translation	MEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKVRRLRLSDVGGPHSYSFCSQPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKLSLSPQHKE

Target ORF information:

RefSeq Version	XM_011528948.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528948.2

ORF Insert Sequence:

ATGGAGGCCT TTGGCAACGC CAAGACCCTG AGGAATGATA ACTCCTCCCG CTTTGGCAAG 
TTCATCCGCA TTCACTTTGG TCCCTCTGGG AAGCTGGCAT CCGCGGATAT TGACAGCTAT 
CTCCTGGAGA AGTCGCGGGT GATCTTCCAG TTGCCTGGTG AGCGCAGCTA CCATGTCTAC 
TACCAGATCC TCTCAGGGAG GAAGCCAGAG CTGCAGGACA TGCTGCTTCT GTCTATGAAC 
CCCTATGACT ACCACTTCTG CAGCCAGGGC GTCATCACCG TGGACAACAT GAATGATGGG 
GAGGAGCTCA TCGCCACCGA CCATGCCATG GACATCCTAG GCTTCAGCGT GGATGAGAAA 
TGTGCCTGCT ATAAGATCGT GGGCGCCCTC CTGCACTTTG GCAACATGAA GTTCAAGCAG 
AAGCAGCGGG AGGAGCAGGC GGAGGCCGAT GGCACTGAGA GTGCTGACAA GGCTGCCTAC 
CTGATGGGGG TCAGCAGTGG GGACCTCCTC AAAGGCCTTT TGCACCCCCG GGTGCGTGTA 
GGGAACGAGT ACGTGACCAA GGGCCAGAGT GTGGAGCAGG TGGTGTTTGC TGTGGGGGCT 
CTGGCCAAGG CCACCTATGA CCGGCTGTTC AGGTGGCTGG TGTCTCGGAT CAACCAGACC 
CTGGACACAA AGCTGCCCCG GCAGTTCTTC ATCGGGGTTC TGGACATCGC TGGGTTTGAG 
ATCTTTGAGT TCAACAGCTT CGAACAGCTG TGCATCAACT TCACCAATGA GAAATTGCAG 
CAGTTCTTCA ACCAGCACAT GTTTGTGCTG GAGCAGGAGG AGTACAAGCG GGAGGGCATC 
GACTGGGTCT TCATCGACTT CGGCCTTGAC CTGCAGCCTT GCATCGACCT CATCGAGAAG 
GTTAGAAGAC TGAGGCTTAG TGATGTCGGA GGGCCCCACA GCTACTCGTT CTGTTCCCAG 
CCACTGGGCA TCCTGTCCAT CCTGGAGGAG GAATGCATGT TCCCCAAGGC CTCAGACGCC 
AGCTTCCGGG CCAAGCTCTA CGACAACCAC GCGGGGAAGT CACCCAATTT CCAGCAGCCT 
CGGCCTGACA AGAAGCGCAA GTACCAGGCC CACTTCGAGG TGGTCCACTA CGCAGGCGTG 
GTGCCTTACA GCATTGTGGG CTGGCTGGAG AAAAACAAGG ATCCCCTGAA TGAGACCGTG 
GTCCCCATCT TCCAGAAGTC ACAGAATAGG CTCCTGGCGA CTCTCTATGA GAATTATGCG 
GGCTCCTGCT CCACTGAGCC CCCCAAGTCT GGGGTGAAAG AGAAGCGTAA GAAGGCAGCA 
TCGTTCCAGA CGGTGTCCCA GCTGCACAAG GAGAACCTCA ACAAGCTGAT GACCAACCTG 
CGGGCCACAC AGCCCCACTT CGTCCGCTGC ATTGTCCCCA ACGAGAACAA AACCCCAGGG 
GTCATGGATG CCTTCTTGGT GCTACACCAG CTGCGCTGCA ATGGGGTCCT GGAGGGGATC 
CGGATCTGCC GCCAAGGGTT CCCCAACAGG TTGCTCTACA CCGACTTCCG GCAGCGGTAC 
CGTATCCTGA ACCCCAGTGC CATCCCGGAT GACACCTTCA TGGACAGCAG GAAGGCCACA 
GAGAAACTGC TGGGCTCGCT GGACTTGGAT CACACCCAGT ACCAGTTTGG CCACACCAAG 
GTGTTCTTCA AGGCTGGGCT TCTAGGCGTC CTGGAAGAGC TCCGTGACCA GCGCCTGGCC 
AAGGTGCTGA CGCTGCTGCA GGCGCGGAGC CGTGGCCGCC TCATGCGCCT TGAGTACCAG 
CGCCTGCTGG GAGGCAGGGA TGCGCTGTTC ACCATCCAGT GGAACATCCG TGCCTTCAAT 
GCCGTCAAGA ACTGGTCATG GATGAAGCTC TTTTTCAAGA TGAAGCCGCT GCTGCGCTCG 
GCGCAGGCTG AGGAGGAGCT GGCGGCCCTG CGGGCAGAGC TGCGGGGGTT GCGAGGGGCG 
CTGGCTGCGG CCGAGGCCAA GCGCCAGGAA CTGGAGGAGA CGCACGTCAG CATCACCCAG 
GAGAAGAATG ACCTGGCCCT GCAGCTGCAG GCTGAGCAGG ACAACCTGGC AGATGCCGAG 
GAGCGCTGCC ACTTGCTGAT CAAGTCCAAG GTGCAGCTGG AGGGGAAGGT GAAGGAGCTG 
AGTGAGCGGC TGGAGGATGA GGAGGAGGTG AACGCTGACC TGGCCGCCCG CCGGCGCAAG 
CTGGAGGACG AGTGCACGGA GCTCAAGAAG GACATTGATG ACCTGGAGCT GACACTGGCC 
AAAGCTGAGA AGGAGAAGCA AGCCACTGAG AACAAGGTGA AGAACCTGAC GGAAGAGATG 
GCTGCGCTGG ACGAGTCAGT GGCCCGGCTG ACCAAGGAGA AGAAGGCGTT GCAGGAGGCC 
CACCAACAGG CCCTGGGTGA CCTGCAGGCC GAGGAGGACC GTGTGAGCGC GCTGACCAAG 
GCCAAGCTCC GGCTGGAGCA ACAGGTGGAG GACCTGGAAT GCTCCCTGGA GCAGGAGAAG 
AAGCTGCGCA TGGACACGGA GCGGGCCAAG CGCAAGCTGG AGGGTGACCT GAAGCTGACG 
CAGGAGTCGG TGGCTGATGC TGCTCAAGAC AAGCAGCAGC TGGAGGAGAA GCTCAAGAAG 
AAGGACTCCG AGCTGAGCCA GCTGAGCCTG CGGGTGGAAG ACGAGCAGCT CTTGGGGGCC 
CAGATGCAGA AGAAGATCAA GGAGCTGCAG GCTCGGGCGG AGGAGCTGGA AGAGGAGCTG 
GAGGCAGAGC GGGCAGCCCG GGCCCGCGTG GAGAAGCAGC GTGCAGAGGC GGCGCGGGAG 
CTGGAGGAGC TGAGCGAGCG GCTGGAGGAG GCAGGCGGCG CATCCGCGGG GCAGCGCGAG 
GGCTGCCGCA AGCGGGAGGC GGAGCTGGGG AGGCTGCGGC GGGAGCTGGA GGAGGCGGCG 
CTGCGGCACG AGGCCACAGT GGCGGCACTG CGGCGCAAGC AGGCGGAGGG CGCGGCGGAG 
CTGGGGGAGC AGGTGGACAG CCTGCAGCGG GTGCGGCAGA AGCTGGAGAA GGAGAAGAGT 
GAGCTGCGCA TGGAGGTGGA CGACCTGGCT GCCAACGTGG AGACTCTGAC CCGCGCCAAG 
GCCAGTGCAG AGAAGCTGTG CCGGACCTAT GAGGATCAGC TAAGCGAGGC CAAGATCAAG 
GTGGAGGAGC TGCAGCGGCA GCTGGCGGAC GCAAGCACGC AGCGTGGGCG ACTACAGACG 
GAAAGCGGGG AGCTGAGTCG CCTGCTAGAG GAGAAGGAGT GTCTGATCAG TCAGCTGAGC 
CGTGGAAAGG CCCTGGCCGC CCAAAGCCTG GAAGAGTTGC GGCGCCAGCT AGAGGAGGAA 
AGCAAGGCCA AGAGTGCCCT GGCCCACGCC GTGCAGGCTC TGCGGCACGA CTGTGACCTC 
CTGCGGGAGC AACACGAGGA GGAGGCTGAG GCCCAGGCTG AGCTGCAGCG GCTGCTGTCC 
AAGGCCAATG CCGAGGTGGC CCAGTGGAGG AGCAAGTACG AAGCAGATGC CATCCAGAGG 
ACCGAGGAGC TGGAGGAGGC CAAAAAAAAG CTGGCACTGC GGCTGCAGGA GGCAGAGGAG 
GGCGTGGAGG CTGCCAACGC CAAGTGCTCA TCGTTGGAGA AGGCCAAGCT GCGGCTACAG 
ACAGAGTCAG AGGATGTAAC CCTGGAGCTG GAGCGGGCGA CCTCAGCAGC TGCTGCGCTG 
GACAAGAAGC AGCGGCACTT GGAACGGGCA CTGGAGGAAC GGCGGCGGCA GGAGGAGGAG 
ATGCAGCGGG AGCTGGAGGC GGCACAGAGG GAGTCCCGTG GCCTGGGCAC CGAGCTCTTC 
CGGCTGCGGC ACGGCCACGA GGAGGCACTT GAAGCCCTGG AGACGCTCAA GCGGGAGAAC 
AAGAACCTGC AGGAGGAGAT CAGCGACCTC ACAGACCAGG TGAGTCTCAG TGGGAAGAGC 
ATCCAGGAAC TGGAGAAAAC CAAGAAGGCG CTGGAAGGCG AGAAGAGTGA GATCCAGGCT 
GCACTGGAGG AGGCAGAGGG GGCCCTGGAG CTGGAGGAGA CCAAGACGCT GCGGATCCAG 
CTGGAGCTCT CCCAGGTCAA AGCAGAAGTG GACCGGAAGC TGGCAGAGAA AGACGAGGAG 
TGCGCTAACC TGAGGCGCAA CCACCAGCGA GCTGTGGAGT CCCTGCAGGC CTCCCTGGAT 
GCAGAGACAC GGGCCCGCAA TGAGGCGCTG CGGCTCAAGA AGAAGATGGA GGGTGACCTC 
AACGACCTGG AGCTGCAGCT GGGCCATGCC ACCCGTCAGG CCACAGAGGC CCAGGCTGCC 
ACGCGGCTGA TGCAGGCACA GCTCAAGGAG GAGCAGGCAG GGCGGGACGA GGAGCAGCGG 
CTGGCAGCTG AGCTCCACGA GCAGGCGCAG GCTCTGGAGC GCCGGGCCTC GCTGCTGGCT 
GCGGAGCTGG AGGAGCTGCG GGCTGCCCTG GAGCAGGGCG AGCGCAGCCG GCGACTGGCA 
GAGCAGGAGC TTTTGGAGGC CACCGAGCGC CTCAACCTTC TGCATTCGCA GAACACAGGC 
CTCCTAAACC AGAAGAAGAA GCTGGAGGCG GACTTGGCCC AGCTGAGCGG GGAGGTGGAG 
GAGGCTGCAC AGGAGAGGCG GGAGGCTGAG GAGAAGGCCA AAAAGGCCAT CACTGATGCG 
GCCATGATGG CCGAGGAGCT GAAGAAGGAG CAGGACACAA GTGCACACCT GGAACGGATG 
AAGAAGACGC TGGAGCAGAC GGTGCGCGAG CTCCAGGCCC GCCTTGAGGA GGCAGAACAG 
GCCGCCCTCC GTGGCGGGAA GAAGCAGGTG CAGAAGCTGG AGGCCAAGGT ACGGGAGCTG 
GAGGCTGAGC TTGATGCAGA GCAGAAGAAG CACGCCGAGG CCCTTAAGGG CGTGCGCAAG 
CATGAGCGCC GTGTCAAGGA GCTCGCATAC CAGGCCGAGG AGGACAGGAA GAACCTGGCT 
CGCATGCAGG ACCTGGTGGA CAAGCTGCAG AGCAAGGTCA AGAGCTACAA GCGCCAGTTT 
GAGGAGGCGG AGCAGCAGGC CAACACCAAC CTGGCCAAGT ATCGCAAGGC CCAGCACGAG 
CTGGATGATG CGGAGGAGCG GGCAGACATG GCGGAAACCC AGGCCAACAA GCTGCGGGCA 
CGGACCCGGG ACGCCCTGGG CCCCAAGCTA TCCCTTTCTC CTCAGCACAA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( XM_011528947.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527249.1 Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X4, mRNA.

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CloneID	OHu70762
Clone ID Related Accession (Same CDS sequence)	XM_011528948.2 , XM_011528947.2
Accession Version	XM_011528947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-7B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCT TTGGCAACGC CAAGACCCTG AGGAATGATA ACTCCTCCCG CTTTGGCAAG 
TTCATCCGCA TTCACTTTGG TCCCTCTGGG AAGCTGGCAT CCGCGGATAT TGACAGCTAT 
CTCCTGGAGA AGTCGCGGGT GATCTTCCAG TTGCCTGGTG AGCGCAGCTA CCATGTCTAC 
TACCAGATCC TCTCAGGGAG GAAGCCAGAG CTGCAGGACA TGCTGCTTCT GTCTATGAAC 
CCCTATGACT ACCACTTCTG CAGCCAGGGC GTCATCACCG TGGACAACAT GAATGATGGG 
GAGGAGCTCA TCGCCACCGA CCATGCCATG GACATCCTAG GCTTCAGCGT GGATGAGAAA 
TGTGCCTGCT ATAAGATCGT GGGCGCCCTC CTGCACTTTG GCAACATGAA GTTCAAGCAG 
AAGCAGCGGG AGGAGCAGGC GGAGGCCGAT GGCACTGAGA GTGCTGACAA GGCTGCCTAC 
CTGATGGGGG TCAGCAGTGG GGACCTCCTC AAAGGCCTTT TGCACCCCCG GGTGCGTGTA 
GGGAACGAGT ACGTGACCAA GGGCCAGAGT GTGGAGCAGG TGGTGTTTGC TGTGGGGGCT 
CTGGCCAAGG CCACCTATGA CCGGCTGTTC AGGTGGCTGG TGTCTCGGAT CAACCAGACC 
CTGGACACAA AGCTGCCCCG GCAGTTCTTC ATCGGGGTTC TGGACATCGC TGGGTTTGAG 
ATCTTTGAGT TCAACAGCTT CGAACAGCTG TGCATCAACT TCACCAATGA GAAATTGCAG 
CAGTTCTTCA ACCAGCACAT GTTTGTGCTG GAGCAGGAGG AGTACAAGCG GGAGGGCATC 
GACTGGGTCT TCATCGACTT CGGCCTTGAC CTGCAGCCTT GCATCGACCT CATCGAGAAG 
GTTAGAAGAC TGAGGCTTAG TGATGTCGGA GGGCCCCACA GCTACTCGTT CTGTTCCCAG 
CCACTGGGCA TCCTGTCCAT CCTGGAGGAG GAATGCATGT TCCCCAAGGC CTCAGACGCC 
AGCTTCCGGG CCAAGCTCTA CGACAACCAC GCGGGGAAGT CACCCAATTT CCAGCAGCCT 
CGGCCTGACA AGAAGCGCAA GTACCAGGCC CACTTCGAGG TGGTCCACTA CGCAGGCGTG 
GTGCCTTACA GCATTGTGGG CTGGCTGGAG AAAAACAAGG ATCCCCTGAA TGAGACCGTG 
GTCCCCATCT TCCAGAAGTC ACAGAATAGG CTCCTGGCGA CTCTCTATGA GAATTATGCG 
GGCTCCTGCT CCACTGAGCC CCCCAAGTCT GGGGTGAAAG AGAAGCGTAA GAAGGCAGCA 
TCGTTCCAGA CGGTGTCCCA GCTGCACAAG GAGAACCTCA ACAAGCTGAT GACCAACCTG 
CGGGCCACAC AGCCCCACTT CGTCCGCTGC ATTGTCCCCA ACGAGAACAA AACCCCAGGG 
GTCATGGATG CCTTCTTGGT GCTACACCAG CTGCGCTGCA ATGGGGTCCT GGAGGGGATC 
CGGATCTGCC GCCAAGGGTT CCCCAACAGG TTGCTCTACA CCGACTTCCG GCAGCGGTAC 
CGTATCCTGA ACCCCAGTGC CATCCCGGAT GACACCTTCA TGGACAGCAG GAAGGCCACA 
GAGAAACTGC TGGGCTCGCT GGACTTGGAT CACACCCAGT ACCAGTTTGG CCACACCAAG 
GTGTTCTTCA AGGCTGGGCT TCTAGGCGTC CTGGAAGAGC TCCGTGACCA GCGCCTGGCC 
AAGGTGCTGA CGCTGCTGCA GGCGCGGAGC CGTGGCCGCC TCATGCGCCT TGAGTACCAG 
CGCCTGCTGG GAGGCAGGGA TGCGCTGTTC ACCATCCAGT GGAACATCCG TGCCTTCAAT 
GCCGTCAAGA ACTGGTCATG GATGAAGCTC TTTTTCAAGA TGAAGCCGCT GCTGCGCTCG 
GCGCAGGCTG AGGAGGAGCT GGCGGCCCTG CGGGCAGAGC TGCGGGGGTT GCGAGGGGCG 
CTGGCTGCGG CCGAGGCCAA GCGCCAGGAA CTGGAGGAGA CGCACGTCAG CATCACCCAG 
GAGAAGAATG ACCTGGCCCT GCAGCTGCAG GCTGAGCAGG ACAACCTGGC AGATGCCGAG 
GAGCGCTGCC ACTTGCTGAT CAAGTCCAAG GTGCAGCTGG AGGGGAAGGT GAAGGAGCTG 
AGTGAGCGGC TGGAGGATGA GGAGGAGGTG AACGCTGACC TGGCCGCCCG CCGGCGCAAG 
CTGGAGGACG AGTGCACGGA GCTCAAGAAG GACATTGATG ACCTGGAGCT GACACTGGCC 
AAAGCTGAGA AGGAGAAGCA AGCCACTGAG AACAAGGTGA AGAACCTGAC GGAAGAGATG 
GCTGCGCTGG ACGAGTCAGT GGCCCGGCTG ACCAAGGAGA AGAAGGCGTT GCAGGAGGCC 
CACCAACAGG CCCTGGGTGA CCTGCAGGCC GAGGAGGACC GTGTGAGCGC GCTGACCAAG 
GCCAAGCTCC GGCTGGAGCA ACAGGTGGAG GACCTGGAAT GCTCCCTGGA GCAGGAGAAG 
AAGCTGCGCA TGGACACGGA GCGGGCCAAG CGCAAGCTGG AGGGTGACCT GAAGCTGACG 
CAGGAGTCGG TGGCTGATGC TGCTCAAGAC AAGCAGCAGC TGGAGGAGAA GCTCAAGAAG 
AAGGACTCCG AGCTGAGCCA GCTGAGCCTG CGGGTGGAAG ACGAGCAGCT CTTGGGGGCC 
CAGATGCAGA AGAAGATCAA GGAGCTGCAG GCTCGGGCGG AGGAGCTGGA AGAGGAGCTG 
GAGGCAGAGC GGGCAGCCCG GGCCCGCGTG GAGAAGCAGC GTGCAGAGGC GGCGCGGGAG 
CTGGAGGAGC TGAGCGAGCG GCTGGAGGAG GCAGGCGGCG CATCCGCGGG GCAGCGCGAG 
GGCTGCCGCA AGCGGGAGGC GGAGCTGGGG AGGCTGCGGC GGGAGCTGGA GGAGGCGGCG 
CTGCGGCACG AGGCCACAGT GGCGGCACTG CGGCGCAAGC AGGCGGAGGG CGCGGCGGAG 
CTGGGGGAGC AGGTGGACAG CCTGCAGCGG GTGCGGCAGA AGCTGGAGAA GGAGAAGAGT 
GAGCTGCGCA TGGAGGTGGA CGACCTGGCT GCCAACGTGG AGACTCTGAC CCGCGCCAAG 
GCCAGTGCAG AGAAGCTGTG CCGGACCTAT GAGGATCAGC TAAGCGAGGC CAAGATCAAG 
GTGGAGGAGC TGCAGCGGCA GCTGGCGGAC GCAAGCACGC AGCGTGGGCG ACTACAGACG 
GAAAGCGGGG AGCTGAGTCG CCTGCTAGAG GAGAAGGAGT GTCTGATCAG TCAGCTGAGC 
CGTGGAAAGG CCCTGGCCGC CCAAAGCCTG GAAGAGTTGC GGCGCCAGCT AGAGGAGGAA 
AGCAAGGCCA AGAGTGCCCT GGCCCACGCC GTGCAGGCTC TGCGGCACGA CTGTGACCTC 
CTGCGGGAGC AACACGAGGA GGAGGCTGAG GCCCAGGCTG AGCTGCAGCG GCTGCTGTCC 
AAGGCCAATG CCGAGGTGGC CCAGTGGAGG AGCAAGTACG AAGCAGATGC CATCCAGAGG 
ACCGAGGAGC TGGAGGAGGC CAAAAAAAAG CTGGCACTGC GGCTGCAGGA GGCAGAGGAG 
GGCGTGGAGG CTGCCAACGC CAAGTGCTCA TCGTTGGAGA AGGCCAAGCT GCGGCTACAG 
ACAGAGTCAG AGGATGTAAC CCTGGAGCTG GAGCGGGCGA CCTCAGCAGC TGCTGCGCTG 
GACAAGAAGC AGCGGCACTT GGAACGGGCA CTGGAGGAAC GGCGGCGGCA GGAGGAGGAG 
ATGCAGCGGG AGCTGGAGGC GGCACAGAGG GAGTCCCGTG GCCTGGGCAC CGAGCTCTTC 
CGGCTGCGGC ACGGCCACGA GGAGGCACTT GAAGCCCTGG AGACGCTCAA GCGGGAGAAC 
AAGAACCTGC AGGAGGAGAT CAGCGACCTC ACAGACCAGG TGAGTCTCAG TGGGAAGAGC 
ATCCAGGAAC TGGAGAAAAC CAAGAAGGCG CTGGAAGGCG AGAAGAGTGA GATCCAGGCT 
GCACTGGAGG AGGCAGAGGG GGCCCTGGAG CTGGAGGAGA CCAAGACGCT GCGGATCCAG 
CTGGAGCTCT CCCAGGTCAA AGCAGAAGTG GACCGGAAGC TGGCAGAGAA AGACGAGGAG 
TGCGCTAACC TGAGGCGCAA CCACCAGCGA GCTGTGGAGT CCCTGCAGGC CTCCCTGGAT 
GCAGAGACAC GGGCCCGCAA TGAGGCGCTG CGGCTCAAGA AGAAGATGGA GGGTGACCTC 
AACGACCTGG AGCTGCAGCT GGGCCATGCC ACCCGTCAGG CCACAGAGGC CCAGGCTGCC 
ACGCGGCTGA TGCAGGCACA GCTCAAGGAG GAGCAGGCAG GGCGGGACGA GGAGCAGCGG 
CTGGCAGCTG AGCTCCACGA GCAGGCGCAG GCTCTGGAGC GCCGGGCCTC GCTGCTGGCT 
GCGGAGCTGG AGGAGCTGCG GGCTGCCCTG GAGCAGGGCG AGCGCAGCCG GCGACTGGCA 
GAGCAGGAGC TTTTGGAGGC CACCGAGCGC CTCAACCTTC TGCATTCGCA GAACACAGGC 
CTCCTAAACC AGAAGAAGAA GCTGGAGGCG GACTTGGCCC AGCTGAGCGG GGAGGTGGAG 
GAGGCTGCAC AGGAGAGGCG GGAGGCTGAG GAGAAGGCCA AAAAGGCCAT CACTGATGCG 
GCCATGATGG CCGAGGAGCT GAAGAAGGAG CAGGACACAA GTGCACACCT GGAACGGATG 
AAGAAGACGC TGGAGCAGAC GGTGCGCGAG CTCCAGGCCC GCCTTGAGGA GGCAGAACAG 
GCCGCCCTCC GTGGCGGGAA GAAGCAGGTG CAGAAGCTGG AGGCCAAGGT ACGGGAGCTG 
GAGGCTGAGC TTGATGCAGA GCAGAAGAAG CACGCCGAGG CCCTTAAGGG CGTGCGCAAG 
CATGAGCGCC GTGTCAAGGA GCTCGCATAC CAGGCCGAGG AGGACAGGAA GAACCTGGCT 
CGCATGCAGG ACCTGGTGGA CAAGCTGCAG AGCAAGGTCA AGAGCTACAA GCGCCAGTTT 
GAGGAGGCGG AGCAGCAGGC CAACACCAAC CTGGCCAAGT ATCGCAAGGC CCAGCACGAG 
CTGGATGATG CGGAGGAGCG GGCAGACATG GCGGAAACCC AGGCCAACAA GCTGCGGGCA 
CGGACCCGGG ACGCCCTGGG CCCCAAGCTA TCCCTTTCTC CTCAGCACAA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527249.1
CDS	1175..6391
Translation	MEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKVRRLRLSDVGGPHSYSFCSQPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKLSLSPQHKE

Target ORF information:

RefSeq Version	XM_011528947.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528947.2

ORF Insert Sequence:

ATGGAGGCCT TTGGCAACGC CAAGACCCTG AGGAATGATA ACTCCTCCCG CTTTGGCAAG 
TTCATCCGCA TTCACTTTGG TCCCTCTGGG AAGCTGGCAT CCGCGGATAT TGACAGCTAT 
CTCCTGGAGA AGTCGCGGGT GATCTTCCAG TTGCCTGGTG AGCGCAGCTA CCATGTCTAC 
TACCAGATCC TCTCAGGGAG GAAGCCAGAG CTGCAGGACA TGCTGCTTCT GTCTATGAAC 
CCCTATGACT ACCACTTCTG CAGCCAGGGC GTCATCACCG TGGACAACAT GAATGATGGG 
GAGGAGCTCA TCGCCACCGA CCATGCCATG GACATCCTAG GCTTCAGCGT GGATGAGAAA 
TGTGCCTGCT ATAAGATCGT GGGCGCCCTC CTGCACTTTG GCAACATGAA GTTCAAGCAG 
AAGCAGCGGG AGGAGCAGGC GGAGGCCGAT GGCACTGAGA GTGCTGACAA GGCTGCCTAC 
CTGATGGGGG TCAGCAGTGG GGACCTCCTC AAAGGCCTTT TGCACCCCCG GGTGCGTGTA 
GGGAACGAGT ACGTGACCAA GGGCCAGAGT GTGGAGCAGG TGGTGTTTGC TGTGGGGGCT 
CTGGCCAAGG CCACCTATGA CCGGCTGTTC AGGTGGCTGG TGTCTCGGAT CAACCAGACC 
CTGGACACAA AGCTGCCCCG GCAGTTCTTC ATCGGGGTTC TGGACATCGC TGGGTTTGAG 
ATCTTTGAGT TCAACAGCTT CGAACAGCTG TGCATCAACT TCACCAATGA GAAATTGCAG 
CAGTTCTTCA ACCAGCACAT GTTTGTGCTG GAGCAGGAGG AGTACAAGCG GGAGGGCATC 
GACTGGGTCT TCATCGACTT CGGCCTTGAC CTGCAGCCTT GCATCGACCT CATCGAGAAG 
GTTAGAAGAC TGAGGCTTAG TGATGTCGGA GGGCCCCACA GCTACTCGTT CTGTTCCCAG 
CCACTGGGCA TCCTGTCCAT CCTGGAGGAG GAATGCATGT TCCCCAAGGC CTCAGACGCC 
AGCTTCCGGG CCAAGCTCTA CGACAACCAC GCGGGGAAGT CACCCAATTT CCAGCAGCCT 
CGGCCTGACA AGAAGCGCAA GTACCAGGCC CACTTCGAGG TGGTCCACTA CGCAGGCGTG 
GTGCCTTACA GCATTGTGGG CTGGCTGGAG AAAAACAAGG ATCCCCTGAA TGAGACCGTG 
GTCCCCATCT TCCAGAAGTC ACAGAATAGG CTCCTGGCGA CTCTCTATGA GAATTATGCG 
GGCTCCTGCT CCACTGAGCC CCCCAAGTCT GGGGTGAAAG AGAAGCGTAA GAAGGCAGCA 
TCGTTCCAGA CGGTGTCCCA GCTGCACAAG GAGAACCTCA ACAAGCTGAT GACCAACCTG 
CGGGCCACAC AGCCCCACTT CGTCCGCTGC ATTGTCCCCA ACGAGAACAA AACCCCAGGG 
GTCATGGATG CCTTCTTGGT GCTACACCAG CTGCGCTGCA ATGGGGTCCT GGAGGGGATC 
CGGATCTGCC GCCAAGGGTT CCCCAACAGG TTGCTCTACA CCGACTTCCG GCAGCGGTAC 
CGTATCCTGA ACCCCAGTGC CATCCCGGAT GACACCTTCA TGGACAGCAG GAAGGCCACA 
GAGAAACTGC TGGGCTCGCT GGACTTGGAT CACACCCAGT ACCAGTTTGG CCACACCAAG 
GTGTTCTTCA AGGCTGGGCT TCTAGGCGTC CTGGAAGAGC TCCGTGACCA GCGCCTGGCC 
AAGGTGCTGA CGCTGCTGCA GGCGCGGAGC CGTGGCCGCC TCATGCGCCT TGAGTACCAG 
CGCCTGCTGG GAGGCAGGGA TGCGCTGTTC ACCATCCAGT GGAACATCCG TGCCTTCAAT 
GCCGTCAAGA ACTGGTCATG GATGAAGCTC TTTTTCAAGA TGAAGCCGCT GCTGCGCTCG 
GCGCAGGCTG AGGAGGAGCT GGCGGCCCTG CGGGCAGAGC TGCGGGGGTT GCGAGGGGCG 
CTGGCTGCGG CCGAGGCCAA GCGCCAGGAA CTGGAGGAGA CGCACGTCAG CATCACCCAG 
GAGAAGAATG ACCTGGCCCT GCAGCTGCAG GCTGAGCAGG ACAACCTGGC AGATGCCGAG 
GAGCGCTGCC ACTTGCTGAT CAAGTCCAAG GTGCAGCTGG AGGGGAAGGT GAAGGAGCTG 
AGTGAGCGGC TGGAGGATGA GGAGGAGGTG AACGCTGACC TGGCCGCCCG CCGGCGCAAG 
CTGGAGGACG AGTGCACGGA GCTCAAGAAG GACATTGATG ACCTGGAGCT GACACTGGCC 
AAAGCTGAGA AGGAGAAGCA AGCCACTGAG AACAAGGTGA AGAACCTGAC GGAAGAGATG 
GCTGCGCTGG ACGAGTCAGT GGCCCGGCTG ACCAAGGAGA AGAAGGCGTT GCAGGAGGCC 
CACCAACAGG CCCTGGGTGA CCTGCAGGCC GAGGAGGACC GTGTGAGCGC GCTGACCAAG 
GCCAAGCTCC GGCTGGAGCA ACAGGTGGAG GACCTGGAAT GCTCCCTGGA GCAGGAGAAG 
AAGCTGCGCA TGGACACGGA GCGGGCCAAG CGCAAGCTGG AGGGTGACCT GAAGCTGACG 
CAGGAGTCGG TGGCTGATGC TGCTCAAGAC AAGCAGCAGC TGGAGGAGAA GCTCAAGAAG 
AAGGACTCCG AGCTGAGCCA GCTGAGCCTG CGGGTGGAAG ACGAGCAGCT CTTGGGGGCC 
CAGATGCAGA AGAAGATCAA GGAGCTGCAG GCTCGGGCGG AGGAGCTGGA AGAGGAGCTG 
GAGGCAGAGC GGGCAGCCCG GGCCCGCGTG GAGAAGCAGC GTGCAGAGGC GGCGCGGGAG 
CTGGAGGAGC TGAGCGAGCG GCTGGAGGAG GCAGGCGGCG CATCCGCGGG GCAGCGCGAG 
GGCTGCCGCA AGCGGGAGGC GGAGCTGGGG AGGCTGCGGC GGGAGCTGGA GGAGGCGGCG 
CTGCGGCACG AGGCCACAGT GGCGGCACTG CGGCGCAAGC AGGCGGAGGG CGCGGCGGAG 
CTGGGGGAGC AGGTGGACAG CCTGCAGCGG GTGCGGCAGA AGCTGGAGAA GGAGAAGAGT 
GAGCTGCGCA TGGAGGTGGA CGACCTGGCT GCCAACGTGG AGACTCTGAC CCGCGCCAAG 
GCCAGTGCAG AGAAGCTGTG CCGGACCTAT GAGGATCAGC TAAGCGAGGC CAAGATCAAG 
GTGGAGGAGC TGCAGCGGCA GCTGGCGGAC GCAAGCACGC AGCGTGGGCG ACTACAGACG 
GAAAGCGGGG AGCTGAGTCG CCTGCTAGAG GAGAAGGAGT GTCTGATCAG TCAGCTGAGC 
CGTGGAAAGG CCCTGGCCGC CCAAAGCCTG GAAGAGTTGC GGCGCCAGCT AGAGGAGGAA 
AGCAAGGCCA AGAGTGCCCT GGCCCACGCC GTGCAGGCTC TGCGGCACGA CTGTGACCTC 
CTGCGGGAGC AACACGAGGA GGAGGCTGAG GCCCAGGCTG AGCTGCAGCG GCTGCTGTCC 
AAGGCCAATG CCGAGGTGGC CCAGTGGAGG AGCAAGTACG AAGCAGATGC CATCCAGAGG 
ACCGAGGAGC TGGAGGAGGC CAAAAAAAAG CTGGCACTGC GGCTGCAGGA GGCAGAGGAG 
GGCGTGGAGG CTGCCAACGC CAAGTGCTCA TCGTTGGAGA AGGCCAAGCT GCGGCTACAG 
ACAGAGTCAG AGGATGTAAC CCTGGAGCTG GAGCGGGCGA CCTCAGCAGC TGCTGCGCTG 
GACAAGAAGC AGCGGCACTT GGAACGGGCA CTGGAGGAAC GGCGGCGGCA GGAGGAGGAG 
ATGCAGCGGG AGCTGGAGGC GGCACAGAGG GAGTCCCGTG GCCTGGGCAC CGAGCTCTTC 
CGGCTGCGGC ACGGCCACGA GGAGGCACTT GAAGCCCTGG AGACGCTCAA GCGGGAGAAC 
AAGAACCTGC AGGAGGAGAT CAGCGACCTC ACAGACCAGG TGAGTCTCAG TGGGAAGAGC 
ATCCAGGAAC TGGAGAAAAC CAAGAAGGCG CTGGAAGGCG AGAAGAGTGA GATCCAGGCT 
GCACTGGAGG AGGCAGAGGG GGCCCTGGAG CTGGAGGAGA CCAAGACGCT GCGGATCCAG 
CTGGAGCTCT CCCAGGTCAA AGCAGAAGTG GACCGGAAGC TGGCAGAGAA AGACGAGGAG 
TGCGCTAACC TGAGGCGCAA CCACCAGCGA GCTGTGGAGT CCCTGCAGGC CTCCCTGGAT 
GCAGAGACAC GGGCCCGCAA TGAGGCGCTG CGGCTCAAGA AGAAGATGGA GGGTGACCTC 
AACGACCTGG AGCTGCAGCT GGGCCATGCC ACCCGTCAGG CCACAGAGGC CCAGGCTGCC 
ACGCGGCTGA TGCAGGCACA GCTCAAGGAG GAGCAGGCAG GGCGGGACGA GGAGCAGCGG 
CTGGCAGCTG AGCTCCACGA GCAGGCGCAG GCTCTGGAGC GCCGGGCCTC GCTGCTGGCT 
GCGGAGCTGG AGGAGCTGCG GGCTGCCCTG GAGCAGGGCG AGCGCAGCCG GCGACTGGCA 
GAGCAGGAGC TTTTGGAGGC CACCGAGCGC CTCAACCTTC TGCATTCGCA GAACACAGGC 
CTCCTAAACC AGAAGAAGAA GCTGGAGGCG GACTTGGCCC AGCTGAGCGG GGAGGTGGAG 
GAGGCTGCAC AGGAGAGGCG GGAGGCTGAG GAGAAGGCCA AAAAGGCCAT CACTGATGCG 
GCCATGATGG CCGAGGAGCT GAAGAAGGAG CAGGACACAA GTGCACACCT GGAACGGATG 
AAGAAGACGC TGGAGCAGAC GGTGCGCGAG CTCCAGGCCC GCCTTGAGGA GGCAGAACAG 
GCCGCCCTCC GTGGCGGGAA GAAGCAGGTG CAGAAGCTGG AGGCCAAGGT ACGGGAGCTG 
GAGGCTGAGC TTGATGCAGA GCAGAAGAAG CACGCCGAGG CCCTTAAGGG CGTGCGCAAG 
CATGAGCGCC GTGTCAAGGA GCTCGCATAC CAGGCCGAGG AGGACAGGAA GAACCTGGCT 
CGCATGCAGG ACCTGGTGGA CAAGCTGCAG AGCAAGGTCA AGAGCTACAA GCGCCAGTTT 
GAGGAGGCGG AGCAGCAGGC CAACACCAAC CTGGCCAAGT ATCGCAAGGC CCAGCACGAG 
CTGGATGATG CGGAGGAGCG GGCAGACATG GCGGAAACCC AGGCCAACAA GCTGCGGGCA 
CGGACCCGGG ACGCCCTGGG CCCCAAGCTA TCCCTTTCTC CTCAGCACAA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7B ( XM_017027986.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883475.1 Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X1, mRNA.

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CloneID	OHu93095
Clone ID Related Accession (Same CDS sequence)	XM_017027986.1
Accession Version	XM_017027986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGGTTAGAA GACTGAGGCT TAGTGATGTC 
GGAGGGCCCC ACAGCTACTC GTTCTGTTCC CAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CACAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883475.1
CDS	25..6036
Translation	MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGYQEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPMNPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVVAAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPSGKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQGVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKVRRLRLSDVGGPHSYSFCSQPLGILSILEEECMFPKASDASFRAKLYDNHAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIPDDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQARSRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQRVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLADASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAHAVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPKHKE

Target ORF information:

RefSeq Version	XM_017027986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7B (MYH7B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027986.1

ORF Insert Sequence:

ATGAGTGGCA ATAAAAGGGG TAGCAGAGCT TCCTGCCCTC ACCGTGGTGC CGAGTGCCTG 
CTGCCTTGGG CCGCCTTGAA CCTCCAGGGT TTCCAGCTCC TCCTCCTTCA CCCCAGTGCC 
ACTGCCATGA TGGATGTGAG TGAACTTGGG GAGTCTGCCC GCTACCTCCG CCAGGGCTAC 
CAGGAGATGA CGAAGGTGCA CACTATCCCA TGGGACGGGA AGAAGCGAGT CTGGGTGCCT 
GATGAACAGG ACGCCTACGT GGAGGCCGAG GTCAAGTCGG AGGCTACCGG GGGCAGAGTC 
ACCGTGGAGA CCAAAGACCA GAAGGTGCTG ATGGTGCGTG AAGCCGAGCT GCAGCCCATG 
AACCCGCCTC GCTTCGACTT ACTGGAGGAC ATGGCCATGA TGACGCACCT GAACGAGGCC 
TCTGTGCTGC ACAACCTGCG CCAGCGCTAT GCCCGCTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACCATCAA CCCCTACAAA TGGCTCCCAG TCTATACGGC CTCCGTAGTG 
GCTGCTTACA AGGGAAAGCG CCGCTCAGAT TCCCCGCCCC ATATATATGC GGTGGCGGAC 
AACGCCTACA ACGACATGCT GCGCAACCGA GACAACCAGT CCATGCTGAT CACCGGAGAG 
TCGGGGGCCG GTAAGACGGT TAACACCAAG CGGGTCATTC AGTACTTTGC CATCGTCGCT 
GCCCTGGGAG ACGGGCCGGG CAAGAAGGCC CAATTTCTGG CAACAAAGAC GGGGGGCACC 
CTTGAGGATC AAATCATCGA GGCCAACCCT GCCATGGAGG CCTTTGGCAA CGCCAAGACC 
CTGAGGAATG ATAACTCCTC CCGCTTTGGC AAGTTCATCC GCATTCACTT TGGTCCCTCT 
GGGAAGCTGG CATCCGCGGA TATTGACAGC TATCTCCTGG AGAAGTCGCG GGTGATCTTC 
CAGTTGCCTG GTGAGCGCAG CTACCATGTC TACTACCAGA TCCTCTCAGG GAGGAAGCCA 
GAGCTGCAGG ACATGCTGCT TCTGTCTATG AACCCCTATG ACTACCACTT CTGCAGCCAG 
GGCGTCATCA CCGTGGACAA CATGAATGAT GGGGAGGAGC TCATCGCCAC CGACCATGCC 
ATGGACATCC TAGGCTTCAG CGTGGATGAG AAATGTGCCT GCTATAAGAT CGTGGGCGCC 
CTCCTGCACT TTGGCAACAT GAAGTTCAAG CAGAAGCAGC GGGAGGAGCA GGCGGAGGCC 
GATGGCACTG AGAGTGCTGA CAAGGCTGCC TACCTGATGG GGGTCAGCAG TGGGGACCTC 
CTCAAAGGCC TTTTGCACCC CCGGGTGCGT GTAGGGAACG AGTACGTGAC CAAGGGCCAG 
AGTGTGGAGC AGGTGGTGTT TGCTGTGGGG GCTCTGGCCA AGGCCACCTA TGACCGGCTG 
TTCAGGTGGC TGGTGTCTCG GATCAACCAG ACCCTGGACA CAAAGCTGCC CCGGCAGTTC 
TTCATCGGGG TTCTGGACAT CGCTGGGTTT GAGATCTTTG AGTTCAACAG CTTCGAACAG 
CTGTGCATCA ACTTCACCAA TGAGAAATTG CAGCAGTTCT TCAACCAGCA CATGTTTGTG 
CTGGAGCAGG AGGAGTACAA GCGGGAGGGC ATCGACTGGG TCTTCATCGA CTTCGGCCTT 
GACCTGCAGC CTTGCATCGA CCTCATCGAG AAGGTTAGAA GACTGAGGCT TAGTGATGTC 
GGAGGGCCCC ACAGCTACTC GTTCTGTTCC CAGCCACTGG GCATCCTGTC CATCCTGGAG 
GAGGAATGCA TGTTCCCCAA GGCCTCAGAC GCCAGCTTCC GGGCCAAGCT CTACGACAAC 
CACGCGGGGA AGTCACCCAA TTTCCAGCAG CCTCGGCCTG ACAAGAAGCG CAAGTACCAG 
GCCCACTTCG AGGTGGTCCA CTACGCAGGC GTGGTGCCTT ACAGCATTGT GGGCTGGCTG 
GAGAAAAACA AGGATCCCCT GAATGAGACC GTGGTCCCCA TCTTCCAGAA GTCACAGAAT 
AGGCTCCTGG CGACTCTCTA TGAGAATTAT GCGGGCTCCT GCTCCACTGA GCCCCCCAAG 
TCTGGGGTGA AAGAGAAGCG TAAGAAGGCA GCATCGTTCC AGACGGTGTC CCAGCTGCAC 
AAGGAGAACC TCAACAAGCT GATGACCAAC CTGCGGGCCA CACAGCCCCA CTTCGTCCGC 
TGCATTGTCC CCAACGAGAA CAAAACCCCA GGGGTCATGG ATGCCTTCTT GGTGCTACAC 
CAGCTGCGCT GCAATGGGGT CCTGGAGGGG ATCCGGATCT GCCGCCAAGG GTTCCCCAAC 
AGGTTGCTCT ACACCGACTT CCGGCAGCGG TACCGTATCC TGAACCCCAG TGCCATCCCG 
GATGACACCT TCATGGACAG CAGGAAGGCC ACAGAGAAAC TGCTGGGCTC GCTGGACTTG 
GATCACACCC AGTACCAGTT TGGCCACACC AAGGTGTTCT TCAAGGCTGG GCTTCTAGGC 
GTCCTGGAAG AGCTCCGTGA CCAGCGCCTG GCCAAGGTGC TGACGCTGCT GCAGGCGCGG 
AGCCGTGGCC GCCTCATGCG CCTTGAGTAC CAGCGCCTGC TGGGAGGCAG GGATGCGCTG 
TTCACCATCC AGTGGAACAT CCGTGCCTTC AATGCCGTCA AGAACTGGTC ATGGATGAAG 
CTCTTTTTCA AGATGAAGCC GCTGCTGCGC TCGGCGCAGG CTGAGGAGGA GCTGGCGGCC 
CTGCGGGCAG AGCTGCGGGG GTTGCGAGGG GCGCTGGCTG CGGCCGAGGC CAAGCGCCAG 
GAACTGGAGG AGACGCACGT CAGCATCACC CAGGAGAAGA ATGACCTGGC CCTGCAGCTG 
CAGGCTGAGC AGGACAACCT GGCAGATGCC GAGGAGCGCT GCCACTTGCT GATCAAGTCC 
AAGGTGCAGC TGGAGGGGAA GGTGAAGGAG CTGAGTGAGC GGCTGGAGGA TGAGGAGGAG 
GTGAACGCTG ACCTGGCCGC CCGCCGGCGC AAGCTGGAGG ACGAGTGCAC GGAGCTCAAG 
AAGGACATTG ATGACCTGGA GCTGACACTG GCCAAAGCTG AGAAGGAGAA GCAAGCCACT 
GAGAACAAGG TGAAGAACCT GACGGAAGAG ATGGCTGCGC TGGACGAGTC AGTGGCCCGG 
CTGACCAAGG AGAAGAAGGC GTTGCAGGAG GCCCACCAAC AGGCCCTGGG TGACCTGCAG 
GCCGAGGAGG ACCGTGTGAG CGCGCTGACC AAGGCCAAGC TCCGGCTGGA GCAACAGGTG 
GAGGACCTGG AATGCTCCCT GGAGCAGGAG AAGAAGCTGC GCATGGACAC GGAGCGGGCC 
AAGCGCAAGC TGGAGGGTGA CCTGAAGCTG ACGCAGGAGT CGGTGGCTGA TGCTGCTCAA 
GACAAGCAGC AGCTGGAGGA GAAGCTCAAG AAGAAGGACT CCGAGCTGAG CCAGCTGAGC 
CTGCGGGTGG AAGACGAGCA GCTCTTGGGG GCCCAGATGC AGAAGAAGAT CAAGGAGCTG 
CAGGCTCGGG CGGAGGAGCT GGAAGAGGAG CTGGAGGCAG AGCGGGCAGC CCGGGCCCGC 
GTGGAGAAGC AGCGTGCAGA GGCGGCGCGG GAGCTGGAGG AGCTGAGCGA GCGGCTGGAG 
GAGGCAGGCG GCGCATCCGC GGGGCAGCGC GAGGGCTGCC GCAAGCGGGA GGCGGAGCTG 
GGGAGGCTGC GGCGGGAGCT GGAGGAGGCG GCGCTGCGGC ACGAGGCCAC AGTGGCGGCA 
CTGCGGCGCA AGCAGGCGGA GGGCGCGGCG GAGCTGGGGG AGCAGGTGGA CAGCCTGCAG 
CGGGTGCGGC AGAAGCTGGA GAAGGAGAAG AGTGAGCTGC GCATGGAGGT GGACGACCTG 
GCTGCCAACG TGGAGACTCT GACCCGCGCC AAGGCCAGTG CAGAGAAGCT GTGCCGGACC 
TATGAGGATC AGCTAAGCGA GGCCAAGATC AAGGTGGAGG AGCTGCAGCG GCAGCTGGCG 
GACGCAAGCA CGCAGCGTGG GCGACTACAG ACGGAAAGCG GGGAGCTGAG TCGCCTGCTA 
GAGGAGAAGG AGTGTCTGAT CAGTCAGCTG AGCCGTGGAA AGGCCCTGGC CGCCCAAAGC 
CTGGAAGAGT TGCGGCGCCA GCTAGAGGAG GAAAGCAAGG CCAAGAGTGC CCTGGCCCAC 
GCCGTGCAGG CTCTGCGGCA CGACTGTGAC CTCCTGCGGG AGCAACACGA GGAGGAGGCT 
GAGGCCCAGG CTGAGCTGCA GCGGCTGCTG TCCAAGGCCA ATGCCGAGGT GGCCCAGTGG 
AGGAGCAAGT ACGAAGCAGA TGCCATCCAG AGGACCGAGG AGCTGGAGGA GGCCAAAAAA 
AAGCTGGCAC TGCGGCTGCA GGAGGCAGAG GAGGGCGTGG AGGCTGCCAA CGCCAAGTGC 
TCATCGTTGG AGAAGGCCAA GCTGCGGCTA CAGACAGAGT CAGAGGATGT AACCCTGGAG 
CTGGAGCGGG CGACCTCAGC AGCTGCTGCG CTGGACAAGA AGCAGCGGCA CTTGGAACGG 
GCACTGGAGG AACGGCGGCG GCAGGAGGAG GAGATGCAGC GGGAGCTGGA GGCGGCACAG 
AGGGAGTCCC GTGGCCTGGG CACCGAGCTC TTCCGGCTGC GGCACGGCCA CGAGGAGGCA 
CTTGAAGCCC TGGAGACGCT CAAGCGGGAG AACAAGAACC TGCAGGAGGA GATCAGCGAC 
CTCACAGACC AGGTGAGTCT CAGTGGGAAG AGCATCCAGG AACTGGAGAA AACCAAGAAG 
GCGCTGGAAG GCGAGAAGAG TGAGATCCAG GCTGCACTGG AGGAGGCAGA GGGGGCCCTG 
GAGCTGGAGG AGACCAAGAC GCTGCGGATC CAGCTGGAGC TCTCCCAGGT CAAAGCAGAA 
GTGGACCGGA AGCTGGCAGA GAAAGACGAG GAGTGCGCTA ACCTGAGGCG CAACCACCAG 
CGAGCTGTGG AGTCCCTGCA GGCCTCCCTG GATGCAGAGA CACGGGCCCG CAATGAGGCG 
CTGCGGCTCA AGAAGAAGAT GGAGGGTGAC CTCAACGACC TGGAGCTGCA GCTGGGCCAT 
GCCACCCGTC AGGCCACAGA GGCCCAGGCT GCCACGCGGC TGATGCAGGC ACAGCTCAAG 
GAGGAGCAGG CAGGGCGGGA CGAGGAGCAG CGGCTGGCAG CTGAGCTCCA CGAGCAGGCG 
CAGGCTCTGG AGCGCCGGGC CTCGCTGCTG GCTGCGGAGC TGGAGGAGCT GCGGGCTGCC 
CTGGAGCAGG GCGAGCGCAG CCGGCGACTG GCAGAGCAGG AGCTTTTGGA GGCCACCGAG 
CGCCTCAACC TTCTGCATTC GCAGAACACA GGCCTCCTAA ACCAGAAGAA GAAGCTGGAG 
GCGGACTTGG CCCAGCTGAG CGGGGAGGTG GAGGAGGCTG CACAGGAGAG GCGGGAGGCT 
GAGGAGAAGG CCAAAAAGGC CATCACTGAT GCGGCCATGA TGGCCGAGGA GCTGAAGAAG 
GAGCAGGACA CAAGTGCACA CCTGGAACGG ATGAAGAAGA CGCTGGAGCA GACGGTGCGC 
GAGCTCCAGG CCCGCCTTGA GGAGGCAGAA CAGGCCGCCC TCCGTGGCGG GAAGAAGCAG 
GTGCAGAAGC TGGAGGCCAA GGTACGGGAG CTGGAGGCTG AGCTTGATGC AGAGCAGAAG 
AAGCACGCCG AGGCCCTTAA GGGCGTGCGC AAGCATGAGC GCCGTGTCAA GGAGCTCGCA 
TACCAGGCCG AGGAGGACAG GAAGAACCTG GCTCGCATGC AGGACCTGGT GGACAAGCTG 
CAGAGCAAGG TCAAGAGCTA CAAGCGCCAG TTTGAGGAGG CGGAGCAGCA GGCCAACACC 
AACCTGGCCA AGTATCGCAA GGCCCAGCAC GAGCTGGATG ATGCGGAGGA GCGGGCAGAC 
ATGGCGGAAA CCCAGGCCAA CAAGCTGCGG GCACGGACCC GGGACGCCCT GGGCCCCAAG 
CACAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myosin-7b Promotes Distal Tip Localization of the Intermicrovillar Adhesion Complex.
Current biology : CB26(20)2717-2728(2016 Oct)
Weck ML,Crawley SW,Stone CR,Tyska MJ

Digenic mutational inheritance of the integrin alpha 7 and the myosin heavy chain 7B genes causes congenital myopathy with left ventricular non-compact cardiomyopathy.
Orphanet journal of rare diseases891(2013 Jun)
Esposito T,Sampaolo S,Limongelli G,Varone A,Formicola D,Diodato D,Farina O,Napolitano F,Pacileo G,Gianfrancesco F,Di Iorio G

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

Genetic associations for activated partial thromboplastin time and prothrombin time, their gene expression profiles, and risk of coronary artery disease.
American journal of human genetics91(1)152-62(2012 Jul)
Tang W,Schwienbacher C,Lopez LM,Ben-Shlomo Y,Oudot-Mellakh T,Johnson AD,Samani NJ,Basu S,G?gele M,Davies G,Lowe GD,Tregouet DA,Tan A,Pankow JS,Tenesa A,Levy D,Volpato CB,Rumley A,Gow AJ,Minelli C,Yarnell JW,Porteous DJ,Starr JM,Gallacher J,Boerwinkle E,Visscher PM,Pramstaller PP,Cushman M,Emilsson V,Plump AS,Matijevic N,Morange PE,Deary IJ,Hicks AA,Folsom AR

Genome wide association study for plasma levels of natural anticoagulant inhibitors and protein C anticoagulant pathway: the MARTHA project.
British journal of haematology157(2)230-9(2012 Apr)
Oudot-Mellakh T,Cohen W,Germain M,Saut N,Kallel C,Zelenika D,Lathrop M,Tr?gou?t DA,Morange PE

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MYH7B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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