SH3RF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SH3RF1
Entrez Gene ID	57630
Full Name	SH3 domain containing ring finger 1
Synonyms	POSH,RNF142,SH3MD2
General protein information	Preferred Names SH3 domain containing ring finger 1 Names E3 ubiquitin-protein ligase SH3RF1 RING-type E3 ubiquitin transferase SH3RF1 SH3 domain-containing RING finger protein 1 SH3 multiple domains 2 SH3 multiple domains protein 2 plenty of SH3 domains plenty of SH3s putative E3 ubiquitin-protein ligase SH3RF1 ring finger protein 142
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.3-q33
Summary	This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_020870.4	NP_065921.2	E3 ubiquitin-protein ligase SH3RF1
NM_020870.3	NP_065921.2	E3 ubiquitin-protein ligase SH3RF1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH3RF1	NP_065921.2
Xenopus tropicalis (tropical clawed frog)	sh3rf1	NP_001015973.1
Mus musculus (house mouse)	Sh3rf1	NP_067481.2
Rattus norvegicus (Norway rat)	Sh3rf1	NP_942059.1
	SH3RF1	XP_001082524.1
Gallus gallus (chicken)	SH3RF1	XP_420402.2
Danio rerio (zebrafish)	sh3rf1	NP_001038952.2
Pan troglodytes (chimpanzee)	SH3RF1	XP_517530.2
Canis lupus familiaris (dog)	SH3RF1	XP_855736.2
Bos taurus (cattle)	SH3RF1	NP_001091534.1

Gene Ontology
Bibliography

Related articles in PubMed

POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.
Wang X, Tian X, Yang Y, Lu X, Li Y, Ma Y, Zhang Y, Zheng F, Lu S, Xu D, Xu X, Wang W, Wang X
Expert opinion on therapeutic targets21(12)1083-1094(2017 Dec)

Protein interaction screening identifies SH3RF1 as a new regulator of FAT1 protein levels.
de Bock CE, Hughes MR, Snyder K, Alley S, Sadeqzadeh E, Dun MD, McNagny KM, Molloy TJ, Hondermarck H, Thorne RF
FEBS letters591(4)667-678(2017 Feb)

Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase.
Ma B, Chen Y, Chen L, Cheng H, Mu C, Li J, Gao R, Zhou C, Cao L, Liu J, Zhu Y, Chen Q, Wu S
Nature cell biology17(1)95-103(2015 Jan)

Imputation-based association analyses identify new lung cancer susceptibility variants in CDK6 and SH3RF1 and their interactions with smoking in Chinese populations.
Deng Q, Guo H, Dai J, Yang L, Wu C, Wang Q, Hu Z, Yang M, Liu L, Yu D, Hu D, Hong X, Qiu F, Yang H, Wang T, Tan W, Chu M, Feng J, Teng K, Gong J, Sun C, Hu X, Zhang K, Lu J, Lin D, Shen H, Wu T
Carcinogenesis34(9)2010-6(2013 Sep)

Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.
Christian PA, Fiandalo MV, Schwarze SR
Molecular cancer1057(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results show that POSH plays a critical role in the progression of epileptic seizures via N-methyl-D-aspartate receptor trafficking
Title: POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.

Data show that the two N-terminal SH3 domains of SH3 domain containing ring finger 1 (SH3RF1) protein interact with FAT1 protein.
Title: Protein interaction screening identifies SH3RF1 as a new regulator of FAT1 protein levels.

We found a genome-wide significant association for rs2282987 in CDK6 and a consistent association for rs2706748 in SH3RF1 .
Title: Imputation-based association analyses identify new lung cancer susceptibility variants in CDK6 and SH3RF1 and their interactions with smoking in Chinese populations.

The data presented here define POSH and Siah2 as important mediators of death receptor mediated apoptosis and suggest targeting the interaction of these two E3 ligases is a promising novel cancer therapeutic strategy.
Title: Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.

POSH promotes cell survival in Drosophila and in human rheumatoid arthritis synovial fibroblasts
Title: POSH promotes cell survival in Drosophila and in human RASF cells.

The following SH3RF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SH3RF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24205	NM_020870.4 Latest version!	Homo sapiens SH3 domain containing ring finger 1 (SH3RF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu24205	NM_020870.3	Homo sapiens SH3 domain containing ring finger 1 (SH3RF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SH3RF1 ( NM_020870.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065921.2 Homo sapiens SH3 domain containing ring finger 1 (SH3RF1), mRNA.

Price & Availability↑

CloneID	OHu24205
Clone ID Related Accession (Same CDS sequence)	NM_020870.4 , NM_020870.3
Accession Version	NM_020870.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SH3RF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC096741.3, AC104783.3 and AC021151.8. On Nov 23, 2018 this sequence version replaced NM_020870.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.134455.1, SRR1660807.41015.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000284637.14/ ENSP00000284637.9 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGAAT CAGCCTTGTT GGATCTTTTG GAGTGTCCGG TGTGTCTAGA GCGCCTTGAT 
GCTTCTGCGA AGGTCTTGCC TTGCCAGCAT ACGTTTTGCA AGCGATGTTT GCTGGGGATC 
GTAGGTTCTC GAAATGAACT CAGATGTCCC GAGTGCAGGA CTCTTGTTGG CTCGGGTGTC 
GAGGAGCTTC CCAGTAACAT CTTGCTGGTC AGACTTCTGG ATGGCATCAA ACAGAGGCCT 
TGGAAACCTG GTCCTGGTGG GGGAAGTGGG ACCAACTGCA CAAATGCATT AAGGTCTCAG 
AGCAGCACTG TGGCTAATTG TAGCTCAAAA GATCTGCAGA GCTCCCAGGG CGGACAGCAG 
CCTCGGGTGC AATCCTGGAG CCCCCCAGTG AGGGGTATAC CTCAGTTACC ATGTGCCAAA 
GCATTATACA ACTATGAAGG AAAAGAGCCT GGAGACCTTA AATTCAGCAA AGGTGACATC 
ATCATTTTGC GAAGACAAGT GGATGAAAAT TGGTACCATG GGGAAGTCAA TGGAATCCAT 
GGCTTTTTCC CCACCAACTT TGTGCAGATT ATTAAACCGT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAAG CAGACAAAGA TTGCCTTCCA 
TTTGCAAAGG ATGATGTTCT GACTGTGATC CGAAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ACAAAATAGG AATATTTCCA ATTTCATATG TTGAGTTTAA CTCGGCTGCT 
AAGCAGCTGA TAGAATGGGA TAAGCCTCCT GTGCCAGGAG TTGATGCTGG AGAATGTTCC 
TCGGCAGCAG CCCAGAGCAG CACTGCCCCA AAGCACTCCG ACACCAAGAA GAACACCAAA 
AAGCGGCACT CCTTCACTTC CCTCACTATG GCCAACAAGT CCTCCCAGGC ATCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCCCCTGTC CTCATCAGCT CCAGCAACCC CACTGCTGCT 
GCACGGATCA GCGAGCTGTC TGGGCTCTCC TGCAGTGCCC CTTCTCAGGT TCATATAAGT 
ACCACCGGGT TAATTGTGAC CCCGCCCCCA AGCAGCCCAG TGACAACTGG CCCCTCGTTT 
ACTTTCCCAT CAGATGTTCC CTACCAAGCT GCCCTTGGAA CTTTGAATCC TCCTCTTCCA 
CCACCCCCTC TCCTGGCTGC CACTGTCCTT GCCTCCACAC CACCAGGCGC CACCGCCGCT 
GCTGCTGCTG CTGGAATGGG ACCGAGGCCC ATGGCAGGAT CCACTGACCA GATTGCACAT 
TTACGGCCGC AGACTCGCCC CAGTGTGTAT GTTGCTATAT ATCCATACAC TCCTCGGAAA 
GAGGATGAAC TAGAGCTGAG AAAAGGGGAG ATGTTTTTAG TGTTTGAGCG CTGCCAGGAT 
GGCTGGTTCA AAGGGACATC CATGCATACC AGCAAGATAG GGGTTTTCCC TGGCAATTAT 
GTGGCACCAG TCACAAGGGC GGTGACAAAT GCTTCCCAAG CTAAAGTCCC TATGTCTACA 
GCTGGCCAGA CAAGTCGGGG AGTGACCATG GTCAGTCCTT CCACGGCAGG AGGGCCTGCC 
CAGAAGCTCC AGGGAAATGG CGTGGCTGGG AGTCCCAGTG TTGTCCCCGC AGCTGTGGTA 
TCAGCAGCTC ACATCCAGAC AAGTCCTCAG GCTAAGGTCT TGTTGCACAT GACGGGGCAA 
ATGACAGTCA ACCAGGCCCG CAATGCTGTG AGGACAGTTG CAGCGCACAA CCAGGAACGC 
CCCACGGCAG CAGTGACACC CATCCAGGTA CAGAATGCCG CCGGCCTCAG CCCTGCATCT 
GTGGGCCTGT CCCATCACTC GCTGGCCTCC CCACAACCTG CGCCTCTGAT GCCAGGCTCA 
GCCACGCACA CTGCTGCCAT CAGTATCAGT CGAGCCAGTG CCCCTCTGGC CTGTGCAGCA 
GCTGCTCCAC TGACTTCCCC AAGCATCACC AGTGCTTCTC TGGAGGCTGA GCCCAGTGGC 
CGGATAGTGA CCGTTCTCCC TGGACTCCCC ACATCTCCTG ACAGTGCTTC ATCAGCTTGT 
GGGAACAGTT CAGCAACCAA ACCAGACAAG GATAGCAAAA AAGAAAAAAA GGGTTTGTTG 
AAGTTGCTTT CTGGCGCCTC CACTAAACGG AAGCCCCGCG TGTCTCCTCC AGCATCGCCC 
ACCCTAGAAG TGGAGCTGGG CAGTGCAGAG CTTCCTCTCC AGGGAGCGGT GGGGCCCGAA 
CTGCCACCAG GAGGTGGCCA TGGCAGGGCA GGCTCCTGCC CTGTGGACGG GGACGGACCG 
GTCACGACTG CAGTGGCAGG AGCAGCCCTG GCCCAGGATG CTTTTCATAG GAAGGCAAGT 
TCCCTGGACT CCGCAGTTCC CATCGCTCCA CCTCCTCGCC AGGCCTGTTC CTCCCTGGGT 
CCTGTCTTGA ATGAGTCTAG ACCTGTCGTT TGTGAAAGGC ACAGGGTGGT GGTTTCCTAT 
CCTCCTCAGA GTGAGGCAGA ACTTGAACTT AAAGAAGGAG ATATTGTGTT TGTTCATAAA 
AAACGAGAGG ATGGCTGGTT CAAAGGCACA TTACAACGTA ATGGGAAAAC TGGCCTTTTC 
CCAGGAAGCT TTGTGGAAAA CATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065921.2
CDS	194..2860
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVEELPSNILLVRLLDGIKQRPWKPGPGGGSGTNCTNALRSQSSTVANCSSKDLQSSQGGQQPRVQSWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVNGIHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDAGECSSAAAQSSTAPKHSDTKKNTKKRHSFTSLTMANKSSQASQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPSFTFPSDVPYQAALGTLNPPLPPPPLLAATVLASTPPGATAAAAAAGMGPRPMAGSTDQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWFKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVPMSTAGQTSRGVTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

Target ORF information:

RefSeq Version	NM_020870.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain containing ring finger 1 (SH3RF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020870.4

ORF Insert Sequence:

ATGGATGAAT CAGCCTTGTT GGATCTTTTG GAGTGTCCGG TGTGTCTAGA GCGCCTTGAT 
GCTTCTGCGA AGGTCTTGCC TTGCCAGCAT ACGTTTTGCA AGCGATGTTT GCTGGGGATC 
GTAGGTTCTC GAAATGAACT CAGATGTCCC GAGTGCAGGA CTCTTGTTGG CTCGGGTGTC 
GAGGAGCTTC CCAGTAACAT CTTGCTGGTC AGACTTCTGG ATGGCATCAA ACAGAGGCCT 
TGGAAACCTG GTCCTGGTGG GGGAAGTGGG ACCAACTGCA CAAATGCATT AAGGTCTCAG 
AGCAGCACTG TGGCTAATTG TAGCTCAAAA GATCTGCAGA GCTCCCAGGG CGGACAGCAG 
CCTCGGGTGC AATCCTGGAG CCCCCCAGTG AGGGGTATAC CTCAGTTACC ATGTGCCAAA 
GCATTATACA ACTATGAAGG AAAAGAGCCT GGAGACCTTA AATTCAGCAA AGGTGACATC 
ATCATTTTGC GAAGACAAGT GGATGAAAAT TGGTACCATG GGGAAGTCAA TGGAATCCAT 
GGCTTTTTCC CCACCAACTT TGTGCAGATT ATTAAACCGT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAAG CAGACAAAGA TTGCCTTCCA 
TTTGCAAAGG ATGATGTTCT GACTGTGATC CGAAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ACAAAATAGG AATATTTCCA ATTTCATATG TTGAGTTTAA CTCGGCTGCT 
AAGCAGCTGA TAGAATGGGA TAAGCCTCCT GTGCCAGGAG TTGATGCTGG AGAATGTTCC 
TCGGCAGCAG CCCAGAGCAG CACTGCCCCA AAGCACTCCG ACACCAAGAA GAACACCAAA 
AAGCGGCACT CCTTCACTTC CCTCACTATG GCCAACAAGT CCTCCCAGGC ATCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCCCCTGTC CTCATCAGCT CCAGCAACCC CACTGCTGCT 
GCACGGATCA GCGAGCTGTC TGGGCTCTCC TGCAGTGCCC CTTCTCAGGT TCATATAAGT 
ACCACCGGGT TAATTGTGAC CCCGCCCCCA AGCAGCCCAG TGACAACTGG CCCCTCGTTT 
ACTTTCCCAT CAGATGTTCC CTACCAAGCT GCCCTTGGAA CTTTGAATCC TCCTCTTCCA 
CCACCCCCTC TCCTGGCTGC CACTGTCCTT GCCTCCACAC CACCAGGCGC CACCGCCGCT 
GCTGCTGCTG CTGGAATGGG ACCGAGGCCC ATGGCAGGAT CCACTGACCA GATTGCACAT 
TTACGGCCGC AGACTCGCCC CAGTGTGTAT GTTGCTATAT ATCCATACAC TCCTCGGAAA 
GAGGATGAAC TAGAGCTGAG AAAAGGGGAG ATGTTTTTAG TGTTTGAGCG CTGCCAGGAT 
GGCTGGTTCA AAGGGACATC CATGCATACC AGCAAGATAG GGGTTTTCCC TGGCAATTAT 
GTGGCACCAG TCACAAGGGC GGTGACAAAT GCTTCCCAAG CTAAAGTCCC TATGTCTACA 
GCTGGCCAGA CAAGTCGGGG AGTGACCATG GTCAGTCCTT CCACGGCAGG AGGGCCTGCC 
CAGAAGCTCC AGGGAAATGG CGTGGCTGGG AGTCCCAGTG TTGTCCCCGC AGCTGTGGTA 
TCAGCAGCTC ACATCCAGAC AAGTCCTCAG GCTAAGGTCT TGTTGCACAT GACGGGGCAA 
ATGACAGTCA ACCAGGCCCG CAATGCTGTG AGGACAGTTG CAGCGCACAA CCAGGAACGC 
CCCACGGCAG CAGTGACACC CATCCAGGTA CAGAATGCCG CCGGCCTCAG CCCTGCATCT 
GTGGGCCTGT CCCATCACTC GCTGGCCTCC CCACAACCTG CGCCTCTGAT GCCAGGCTCA 
GCCACGCACA CTGCTGCCAT CAGTATCAGT CGAGCCAGTG CCCCTCTGGC CTGTGCAGCA 
GCTGCTCCAC TGACTTCCCC AAGCATCACC AGTGCTTCTC TGGAGGCTGA GCCCAGTGGC 
CGGATAGTGA CCGTTCTCCC TGGACTCCCC ACATCTCCTG ACAGTGCTTC ATCAGCTTGT 
GGGAACAGTT CAGCAACCAA ACCAGACAAG GATAGCAAAA AAGAAAAAAA GGGTTTGTTG 
AAGTTGCTTT CTGGCGCCTC CACTAAACGG AAGCCCCGCG TGTCTCCTCC AGCATCGCCC 
ACCCTAGAAG TGGAGCTGGG CAGTGCAGAG CTTCCTCTCC AGGGAGCGGT GGGGCCCGAA 
CTGCCACCAG GAGGTGGCCA TGGCAGGGCA GGCTCCTGCC CTGTGGACGG GGACGGACCG 
GTCACGACTG CAGTGGCAGG AGCAGCCCTG GCCCAGGATG CTTTTCATAG GAAGGCAAGT 
TCCCTGGACT CCGCAGTTCC CATCGCTCCA CCTCCTCGCC AGGCCTGTTC CTCCCTGGGT 
CCTGTCTTGA ATGAGTCTAG ACCTGTCGTT TGTGAAAGGC ACAGGGTGGT GGTTTCCTAT 
CCTCCTCAGA GTGAGGCAGA ACTTGAACTT AAAGAAGGAG ATATTGTGTT TGTTCATAAA 
AAACGAGAGG ATGGCTGGTT CAAAGGCACA TTACAACGTA ATGGGAAAAC TGGCCTTTTC 
CCAGGAAGCT TTGTGGAAAA CATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3RF1 ( NM_020870.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065921.2 Homo sapiens SH3 domain containing ring finger 1 (SH3RF1), mRNA.

Price & Availability↑

CloneID	OHu24205
Clone ID Related Accession (Same CDS sequence)	NM_020870.4 , NM_020870.3
Accession Version	NM_020870.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SH3RF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA292937.1, AK021429.1, BC041023.1, AB040927.1 and AC021151.8. On Mar 2, 2006 this sequence version replaced NM_020870.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAAT CAGCCTTGTT GGATCTTTTG GAGTGTCCGG TGTGTCTAGA GCGCCTTGAT 
GCTTCTGCGA AGGTCTTGCC TTGCCAGCAT ACGTTTTGCA AGCGATGTTT GCTGGGGATC 
GTAGGTTCTC GAAATGAACT CAGATGTCCC GAGTGCAGGA CTCTTGTTGG CTCGGGTGTC 
GAGGAGCTTC CCAGTAACAT CTTGCTGGTC AGACTTCTGG ATGGCATCAA ACAGAGGCCT 
TGGAAACCTG GTCCTGGTGG GGGAAGTGGG ACCAACTGCA CAAATGCATT AAGGTCTCAG 
AGCAGCACTG TGGCTAATTG TAGCTCAAAA GATCTGCAGA GCTCCCAGGG CGGACAGCAG 
CCTCGGGTGC AATCCTGGAG CCCCCCAGTG AGGGGTATAC CTCAGTTACC ATGTGCCAAA 
GCATTATACA ACTATGAAGG AAAAGAGCCT GGAGACCTTA AATTCAGCAA AGGTGACATC 
ATCATTTTGC GAAGACAAGT GGATGAAAAT TGGTACCATG GGGAAGTCAA TGGAATCCAT 
GGCTTTTTCC CCACCAACTT TGTGCAGATT ATTAAACCGT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAAG CAGACAAAGA TTGCCTTCCA 
TTTGCAAAGG ATGATGTTCT GACTGTGATC CGAAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ACAAAATAGG AATATTTCCA ATTTCATATG TTGAGTTTAA CTCGGCTGCT 
AAGCAGCTGA TAGAATGGGA TAAGCCTCCT GTGCCAGGAG TTGATGCTGG AGAATGTTCC 
TCGGCAGCAG CCCAGAGCAG CACTGCCCCA AAGCACTCCG ACACCAAGAA GAACACCAAA 
AAGCGGCACT CCTTCACTTC CCTCACTATG GCCAACAAGT CCTCCCAGGC ATCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCCCCTGTC CTCATCAGCT CCAGCAACCC CACTGCTGCT 
GCACGGATCA GCGAGCTGTC TGGGCTCTCC TGCAGTGCCC CTTCTCAGGT TCATATAAGT 
ACCACCGGGT TAATTGTGAC CCCGCCCCCA AGCAGCCCAG TGACAACTGG CCCCTCGTTT 
ACTTTCCCAT CAGATGTTCC CTACCAAGCT GCCCTTGGAA CTTTGAATCC TCCTCTTCCA 
CCACCCCCTC TCCTGGCTGC CACTGTCCTT GCCTCCACAC CACCAGGCGC CACCGCCGCT 
GCTGCTGCTG CTGGAATGGG ACCGAGGCCC ATGGCAGGAT CCACTGACCA GATTGCACAT 
TTACGGCCGC AGACTCGCCC CAGTGTGTAT GTTGCTATAT ATCCATACAC TCCTCGGAAA 
GAGGATGAAC TAGAGCTGAG AAAAGGGGAG ATGTTTTTAG TGTTTGAGCG CTGCCAGGAT 
GGCTGGTTCA AAGGGACATC CATGCATACC AGCAAGATAG GGGTTTTCCC TGGCAATTAT 
GTGGCACCAG TCACAAGGGC GGTGACAAAT GCTTCCCAAG CTAAAGTCCC TATGTCTACA 
GCTGGCCAGA CAAGTCGGGG AGTGACCATG GTCAGTCCTT CCACGGCAGG AGGGCCTGCC 
CAGAAGCTCC AGGGAAATGG CGTGGCTGGG AGTCCCAGTG TTGTCCCCGC AGCTGTGGTA 
TCAGCAGCTC ACATCCAGAC AAGTCCTCAG GCTAAGGTCT TGTTGCACAT GACGGGGCAA 
ATGACAGTCA ACCAGGCCCG CAATGCTGTG AGGACAGTTG CAGCGCACAA CCAGGAACGC 
CCCACGGCAG CAGTGACACC CATCCAGGTA CAGAATGCCG CCGGCCTCAG CCCTGCATCT 
GTGGGCCTGT CCCATCACTC GCTGGCCTCC CCACAACCTG CGCCTCTGAT GCCAGGCTCA 
GCCACGCACA CTGCTGCCAT CAGTATCAGT CGAGCCAGTG CCCCTCTGGC CTGTGCAGCA 
GCTGCTCCAC TGACTTCCCC AAGCATCACC AGTGCTTCTC TGGAGGCTGA GCCCAGTGGC 
CGGATAGTGA CCGTTCTCCC TGGACTCCCC ACATCTCCTG ACAGTGCTTC ATCAGCTTGT 
GGGAACAGTT CAGCAACCAA ACCAGACAAG GATAGCAAAA AAGAAAAAAA GGGTTTGTTG 
AAGTTGCTTT CTGGCGCCTC CACTAAACGG AAGCCCCGCG TGTCTCCTCC AGCATCGCCC 
ACCCTAGAAG TGGAGCTGGG CAGTGCAGAG CTTCCTCTCC AGGGAGCGGT GGGGCCCGAA 
CTGCCACCAG GAGGTGGCCA TGGCAGGGCA GGCTCCTGCC CTGTGGACGG GGACGGACCG 
GTCACGACTG CAGTGGCAGG AGCAGCCCTG GCCCAGGATG CTTTTCATAG GAAGGCAAGT 
TCCCTGGACT CCGCAGTTCC CATCGCTCCA CCTCCTCGCC AGGCCTGTTC CTCCCTGGGT 
CCTGTCTTGA ATGAGTCTAG ACCTGTCGTT TGTGAAAGGC ACAGGGTGGT GGTTTCCTAT 
CCTCCTCAGA GTGAGGCAGA ACTTGAACTT AAAGAAGGAG ATATTGTGTT TGTTCATAAA 
AAACGAGAGG ATGGCTGGTT CAAAGGCACA TTACAACGTA ATGGGAAAAC TGGCCTTTTC 
CCAGGAAGCT TTGTGGAAAA CATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065921.2
CDS	336..3002
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVEELPSNILLVRLLDGIKQRPWKPGPGGGSGTNCTNALRSQSSTVANCSSKDLQSSQGGQQPRVQSWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVNGIHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDAGECSSAAAQSSTAPKHSDTKKNTKKRHSFTSLTMANKSSQASQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPSFTFPSDVPYQAALGTLNPPLPPPPLLAATVLASTPPGATAAAAAAGMGPRPMAGSTDQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWFKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVPMSTAGQTSRGVTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

Target ORF information:

RefSeq Version	NM_020870.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain containing ring finger 1 (SH3RF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020870.3

ORF Insert Sequence:

ATGGATGAAT CAGCCTTGTT GGATCTTTTG GAGTGTCCGG TGTGTCTAGA GCGCCTTGAT 
GCTTCTGCGA AGGTCTTGCC TTGCCAGCAT ACGTTTTGCA AGCGATGTTT GCTGGGGATC 
GTAGGTTCTC GAAATGAACT CAGATGTCCC GAGTGCAGGA CTCTTGTTGG CTCGGGTGTC 
GAGGAGCTTC CCAGTAACAT CTTGCTGGTC AGACTTCTGG ATGGCATCAA ACAGAGGCCT 
TGGAAACCTG GTCCTGGTGG GGGAAGTGGG ACCAACTGCA CAAATGCATT AAGGTCTCAG 
AGCAGCACTG TGGCTAATTG TAGCTCAAAA GATCTGCAGA GCTCCCAGGG CGGACAGCAG 
CCTCGGGTGC AATCCTGGAG CCCCCCAGTG AGGGGTATAC CTCAGTTACC ATGTGCCAAA 
GCATTATACA ACTATGAAGG AAAAGAGCCT GGAGACCTTA AATTCAGCAA AGGTGACATC 
ATCATTTTGC GAAGACAAGT GGATGAAAAT TGGTACCATG GGGAAGTCAA TGGAATCCAT 
GGCTTTTTCC CCACCAACTT TGTGCAGATT ATTAAACCGT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAAG CAGACAAAGA TTGCCTTCCA 
TTTGCAAAGG ATGATGTTCT GACTGTGATC CGAAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ACAAAATAGG AATATTTCCA ATTTCATATG TTGAGTTTAA CTCGGCTGCT 
AAGCAGCTGA TAGAATGGGA TAAGCCTCCT GTGCCAGGAG TTGATGCTGG AGAATGTTCC 
TCGGCAGCAG CCCAGAGCAG CACTGCCCCA AAGCACTCCG ACACCAAGAA GAACACCAAA 
AAGCGGCACT CCTTCACTTC CCTCACTATG GCCAACAAGT CCTCCCAGGC ATCCCAGAAC 
CGCCACTCCA TGGAGATCAG CCCCCCTGTC CTCATCAGCT CCAGCAACCC CACTGCTGCT 
GCACGGATCA GCGAGCTGTC TGGGCTCTCC TGCAGTGCCC CTTCTCAGGT TCATATAAGT 
ACCACCGGGT TAATTGTGAC CCCGCCCCCA AGCAGCCCAG TGACAACTGG CCCCTCGTTT 
ACTTTCCCAT CAGATGTTCC CTACCAAGCT GCCCTTGGAA CTTTGAATCC TCCTCTTCCA 
CCACCCCCTC TCCTGGCTGC CACTGTCCTT GCCTCCACAC CACCAGGCGC CACCGCCGCT 
GCTGCTGCTG CTGGAATGGG ACCGAGGCCC ATGGCAGGAT CCACTGACCA GATTGCACAT 
TTACGGCCGC AGACTCGCCC CAGTGTGTAT GTTGCTATAT ATCCATACAC TCCTCGGAAA 
GAGGATGAAC TAGAGCTGAG AAAAGGGGAG ATGTTTTTAG TGTTTGAGCG CTGCCAGGAT 
GGCTGGTTCA AAGGGACATC CATGCATACC AGCAAGATAG GGGTTTTCCC TGGCAATTAT 
GTGGCACCAG TCACAAGGGC GGTGACAAAT GCTTCCCAAG CTAAAGTCCC TATGTCTACA 
GCTGGCCAGA CAAGTCGGGG AGTGACCATG GTCAGTCCTT CCACGGCAGG AGGGCCTGCC 
CAGAAGCTCC AGGGAAATGG CGTGGCTGGG AGTCCCAGTG TTGTCCCCGC AGCTGTGGTA 
TCAGCAGCTC ACATCCAGAC AAGTCCTCAG GCTAAGGTCT TGTTGCACAT GACGGGGCAA 
ATGACAGTCA ACCAGGCCCG CAATGCTGTG AGGACAGTTG CAGCGCACAA CCAGGAACGC 
CCCACGGCAG CAGTGACACC CATCCAGGTA CAGAATGCCG CCGGCCTCAG CCCTGCATCT 
GTGGGCCTGT CCCATCACTC GCTGGCCTCC CCACAACCTG CGCCTCTGAT GCCAGGCTCA 
GCCACGCACA CTGCTGCCAT CAGTATCAGT CGAGCCAGTG CCCCTCTGGC CTGTGCAGCA 
GCTGCTCCAC TGACTTCCCC AAGCATCACC AGTGCTTCTC TGGAGGCTGA GCCCAGTGGC 
CGGATAGTGA CCGTTCTCCC TGGACTCCCC ACATCTCCTG ACAGTGCTTC ATCAGCTTGT 
GGGAACAGTT CAGCAACCAA ACCAGACAAG GATAGCAAAA AAGAAAAAAA GGGTTTGTTG 
AAGTTGCTTT CTGGCGCCTC CACTAAACGG AAGCCCCGCG TGTCTCCTCC AGCATCGCCC 
ACCCTAGAAG TGGAGCTGGG CAGTGCAGAG CTTCCTCTCC AGGGAGCGGT GGGGCCCGAA 
CTGCCACCAG GAGGTGGCCA TGGCAGGGCA GGCTCCTGCC CTGTGGACGG GGACGGACCG 
GTCACGACTG CAGTGGCAGG AGCAGCCCTG GCCCAGGATG CTTTTCATAG GAAGGCAAGT 
TCCCTGGACT CCGCAGTTCC CATCGCTCCA CCTCCTCGCC AGGCCTGTTC CTCCCTGGGT 
CCTGTCTTGA ATGAGTCTAG ACCTGTCGTT TGTGAAAGGC ACAGGGTGGT GGTTTCCTAT 
CCTCCTCAGA GTGAGGCAGA ACTTGAACTT AAAGAAGGAG ATATTGTGTT TGTTCATAAA 
AAACGAGAGG ATGGCTGGTT CAAAGGCACA TTACAACGTA ATGGGAAAAC TGGCCTTTTC 
CCAGGAAGCT TTGTGGAAAA CATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.
Expert opinion on therapeutic targets21(12)1083-1094(2017 Dec)
Wang X,Tian X,Yang Y,Lu X,Li Y,Ma Y,Zhang Y,Zheng F,Lu S,Xu D,Xu X,Wang W,Wang X

Protein interaction screening identifies SH3RF1 as a new regulator of FAT1 protein levels.
FEBS letters591(4)667-678(2017 Feb)
de Bock CE,Hughes MR,Snyder K,Alley S,Sadeqzadeh E,Dun MD,McNagny KM,Molloy TJ,Hondermarck H,Thorne RF

Hypoxia regulates Hippo signalling through the SIAH2 ubiquitin E3 ligase.
Nature cell biology17(1)95-103(2015 Jan)
Ma B,Chen Y,Chen L,Cheng H,Mu C,Li J,Gao R,Zhou C,Cao L,Liu J,Zhu Y,Chen Q,Wu S

Imputation-based association analyses identify new lung cancer susceptibility variants in CDK6 and SH3RF1 and their interactions with smoking in Chinese populations.
Carcinogenesis34(9)2010-6(2013 Sep)
Deng Q,Guo H,Dai J,Yang L,Wu C,Wang Q,Hu Z,Yang M,Liu L,Yu D,Hu D,Hong X,Qiu F,Yang H,Wang T,Tan W,Chu M,Feng J,Teng K,Gong J,Sun C,Hu X,Zhang K,Lu J,Lin D,Shen H,Wu T

Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.
Molecular cancer1057(2011 May)
Christian PA,Fiandalo MV,Schwarze SR

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SH3RF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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