JCAD gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011519609.2	XP_011517911.1	junctional protein associated with coronary artery disease isoform X2
NM_001350001.1	NP_001336930.1	junctional protein associated with coronary artery disease isoform 2
NM_001350021.1	NP_001336950.1	junctional protein associated with coronary artery disease isoform 2
XM_011519608.1	XP_011517910.1	junctional protein associated with coronary artery disease isoform X1
NM_020848.4	NP_065899.1	junctional protein associated with coronary artery disease isoform 1
NM_001350001.2	NP_001336930.1	junctional protein associated with coronary artery disease isoform 2
NM_001350022.2	NP_001336951.1	junctional protein associated with coronary artery disease isoform 1
NM_001350022.1	NP_001336951.1	junctional protein associated with coronary artery disease isoform 1
NM_020848.3	NP_065899.1	junctional protein associated with coronary artery disease isoform 1
NM_001350021.2	NP_001336950.1	junctional protein associated with coronary artery disease isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22675	NM_020848.4 Latest version!	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu22675	NM_001350022.2 Latest version!	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu22675	XM_011519608.1 Latest version!	Homo sapiens KIAA1462 (KIAA1462), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu43833	NM_001350021.2 Latest version!	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu43833	NM_001350001.2 Latest version!	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu43833	XM_011519609.2 Latest version!	Homo sapiens KIAA1462 (KIAA1462), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu43833	NM_001350001.1	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu22675	NM_001350022.1	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu22675	NM_020848.3	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu43833	NM_001350021.1	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

JCAD ( NM_020848.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065899.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22675
Clone ID Related Accession (Same CDS sequence)	XM_011519608.1 , NM_020848.4 , NM_001350022.2 , NM_001350022.1 , NM_020848.3
Accession Version	NM_020848.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158036.14 and CB988996.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_020848.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.100409.1, SRR1803614.16211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2467143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375377.2/ ENSP00000364526.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065899.1
CDS	165..4244
Translation	MYSVEDLLISHGYKLSRDPPASREDNPKGRQAARTGTRAGQGLQNGHEDGPAALAHRKTSAGKGHVSDSESRRSTPRGHGEPQSTSASRTSEAGFCNQPPSAWSSHPPTGNDQAYRRRGRQEARSQKPREHENLEARGMAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_020848.4
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020848.4

ORF Insert Sequence:

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_001350022.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336951.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22675
Clone ID Related Accession (Same CDS sequence)	XM_011519608.1 , NM_020848.4 , NM_001350022.2 , NM_001350022.1 , NM_020848.3
Accession Version	NM_001350022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360172.17 and AL158036.14. On May 31, 2019 this sequence version replaced NM_001350022.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.252914.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267780 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336951.1
CDS	310..4389
Translation	MYSVEDLLISHGYKLSRDPPASREDNPKGRQAARTGTRAGQGLQNGHEDGPAALAHRKTSAGKGHVSDSESRRSTPRGHGEPQSTSASRTSEAGFCNQPPSAWSSHPPTGNDQAYRRRGRQEARSQKPREHENLEARGMAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_001350022.2
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350022.2

ORF Insert Sequence:

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_001350022.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336951.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 4, mRNA.

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CloneID	OHu22675
Clone ID Related Accession (Same CDS sequence)	XM_011519608.1 , NM_020848.4 , NM_001350022.2 , NM_001350022.1 , NM_020848.3
Accession Version	NM_001350022.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360172.17, AL158036.14 and AL050154.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.252914.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03267780 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336951.1
CDS	310..4389
Translation	MYSVEDLLISHGYKLSRDPPASREDNPKGRQAARTGTRAGQGLQNGHEDGPAALAHRKTSAGKGHVSDSESRRSTPRGHGEPQSTSASRTSEAGFCNQPPSAWSSHPPTGNDQAYRRRGRQEARSQKPREHENLEARGMAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_001350022.1
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350022.1

ORF Insert Sequence:

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_020848.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065899.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 1, mRNA.

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CloneID	OHu22675
Clone ID Related Accession (Same CDS sequence)	XM_011519608.1 , NM_020848.4 , NM_001350022.2 , NM_001350022.1 , NM_020848.3
Accession Version	NM_020848.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158036.14, CB988996.1 and AL050154.1. This sequence is a reference standard in the RefSeqGene project. On Apr 1, 2017 this sequence version replaced NM_020848.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. COMPLETENESS: complete on the 3' end.

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065899.1
CDS	165..4244
Translation	MYSVEDLLISHGYKLSRDPPASREDNPKGRQAARTGTRAGQGLQNGHEDGPAALAHRKTSAGKGHVSDSESRRSTPRGHGEPQSTSASRTSEAGFCNQPPSAWSSHPPTGNDQAYRRRGRQEARSQKPREHENLEARGMAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_020848.3
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020848.3

ORF Insert Sequence:

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( XM_011519608.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517910.1 Homo sapiens KIAA1462 (KIAA1462), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22675
Clone ID Related Accession (Same CDS sequence)	XM_011519608.1 , NM_020848.4 , NM_001350022.2 , NM_001350022.1 , NM_020848.3
Accession Version	XM_011519608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517910.1
CDS	177..4256
Translation	MYSVEDLLISHGYKLSRDPPASREDNPKGRQAARTGTRAGQGLQNGHEDGPAALAHRKTSAGKGHVSDSESRRSTPRGHGEPQSTSASRTSEAGFCNQPPSAWSSHPPTGNDQAYRRRGRQEARSQKPREHENLEARGMAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	XM_011519608.1
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1462 (KIAA1462), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519608.1

ORF Insert Sequence:

ATGTACAGTG TAGAAGACCT CCTGATCTCT CATGGATACA AGCTGTCAAG AGACCCCCCA 
GCATCACGCG AGGATAACCC CAAGGGGCGC CAGGCAGCGA GGACTGGGAC ACGAGCAGGC 
CAGGGCCTGC AGAACGGGCA TGAGGATGGC CCTGCGGCCC TCGCACATCG TAAGACGTCC 
GCGGGGAAAG GACATGTGAG TGACTCCGAA AGCCGCCGCA GCACACCGAG AGGCCACGGG 
GAGCCCCAGA GCACTTCTGC TTCCAGAACC TCGGAGGCGG GGTTTTGTAA TCAACCCCCC 
TCAGCATGGT CCTCTCATCC CCCGACTGGT AACGACCAAG CCTACCGGAG AAGAGGACGG 
CAAGAAGCCA GGAGCCAGAA GCCGAGGGAG CACGAAAACC TGGAGGCCAG AGGAATGGCC 
CAAGCCCACA GCCTGCCTGT CCACGTGAGG GAGGGTCCAT GGGAAGTTGG AGGAAGGTCA 
GAGCATGTGA TGAAGAAGCC AGTTTGGGAA GAAGAATTGC GAATGTCAGG TCCTGCCAAG 
TGGCAGAACG TCAGCCTGGA AAGCTGGAAC CAGCCAAGGA AATTAGGGAG GCAGATGTCT 
GATGGAGATG GGGAGAGACT GTTTCAAGAC CTGTACCCAT TCATTCAAGG AGAACATGTG 
TTGAATTCTC AAAACAAAGG GAAGTCTCGC TCACTGCCTA GAGTTCTTTC CCCCGAGAGC 
CTGAGTTGCA CGGAAATTCC CATTCCATTA AATGAAAGAC ATTCACCTAA AATGCCACCG 
TATCCTCCCA CTTGCGCACC AAATTTGGAC TCCACGAGGA ATTCTGAGAA GAGTGGCTGC 
TCAGCCCCAT TTCCCCGGCC TAAGTTTGGG AGGCCCCTCA AGCCCCCATC TTACAGCTCG 
CACCAGCAGT CTAGGGGAGG AGCGGACAGC AGTGACTCTC AGGACAGCCA GCAGATGGAC 
GCCTATGTCC CCAGGCATGA GCTCTGCCTG TCAGACCCTG GATTGGAACC TCCAGTGTAC 
GTGCCTCCGC CCTCATACAG ATCGCCCCCG CAGAACATCC CAAACCCCTA CTTGGAAGAC 
ACGGTGCCCA TAAATGTGTG TGGCGGTCAC AGTCAACAGC AGTCTCCGAC CGAGAAGGCT 
GGGGCCAGCG GTCAGCCTCC TTCAGGCCCC CCTGGAACTG GGAATGAGTA TGGTGTGAGC 
CCCCGCTTGC CTCAGGGGCT CCCCGCACAT CCCCGACCTG TCACTGCCTA TGACGGCTTC 
GTTCAGTACA TTCCCTTTGA TGATCCACGG TTACGACATT TTAAACTAGC TCAGCCCCAG 
GGTTTCTGTG AAGACATAAA GCTTGACGAT AAATCATATA ACTCCAGTCC TGTCACTGCT 
CAAGAGCCGG CTCATGGAGG AATGCAGCCT GATGGTGCCA TTTGGAATCC ACAGAGCTTA 
ATACCCCCGT CGGGGGATGA GAGAGGCCTG GTCTTGGCCG ATTCCAGCCC CCGGTGGCTG 
TGGGGCCAGC CCCCCGGGGA TGGGGAAAAC AGTGGCCTCC CCAACCAGAG AGACCGCTGT 
GTGGCAAGGG GACAGTGGCC TGATGTGAGA GGCAGCCAGC ACGGGCACAC TGGAAGACAA 
GTTTCCTCCC CTTACTCACA GGGCGAGAGC ACCTGCGAAA CTCAAACCAA GCTCAAAAAG 
TTCCAAACTG GGACTCGGAC CAAGAAAAGT TCAAAGAAAA AAATGAACGA GACTATATTT 
TGCTTGGTTT CTATCCCAGT GAAATCAGAA TCACATCTGC CAGATAGAGA TATGGACAAC 
AATGACTTAA AGCCCAGTGC TGATCAAAAG AATGGGTCTG ATAAGAGCCC GGCTCTGCAA 
GAACAGAGTC TGCTGAGCAT GTCTTCCACC GACCTGGAGC TGCAGGCCCT CACAGGAAGC 
ATGGGTGGGA GAACGGAGTT CCAAAAACAA GATCTAGGGG AACCAGAAGA AGACAGACAA 
ACAAATGACC TCAGTTTCAT CCACCTTACA AAGCACAGAG AACTCAAGCA TTCTGGCTCT 
TGGCCAGGGC ACCGGTACAG AGATCAGCAA ACACAAACCA GTTTCTCCGA GGAGCCCCAA 
AGTTCGCAGC TGCTCCCTGG TGCAAAGCTG GGAGGGCCGA GTCGTGCAGC ATTGAGTCCA 
AAATGTTCAG ACCCTGCTGC CTCCGAAGCT CAGACGCACA CAGCATTCCC TACCGGTGAT 
CACAAACAGA GGCCAAGTGC CCGTAACCTG AAAGGTCACA GGTCCCTCAG CCCATCCAGC 
AACAGTGCGT TCTCAAGGAC TTCCTTGTCC GTGGACCAGG CACCGACGCC AAAAGCAGGC 
CGAAGTCAGC CCTGCGTGGA TGTCCACGGG CTTGGAGCCC ACCCTGGGCC TAAGCGGGAG 
GTGGTGAAGG GGGAGCCCAC GGGCCCTTGC AACAGTAAAC AACTCTTTGG GCAGTTTCTC 
CTGAAACCGG TCAGCCGTCG TCCCTGGGAT TTGATCAGTC AGTTAGAAAG TTTTAACAAG 
GAGCTCCAGG AAGAGGAAGA AAGCAGCAGT AGCAGCAGCA GCAGCAGCAG CAGCAGTGAG 
GAGAGTGAGG CGGAGCCGCA GCAGGAGAAC CGTGCTCACT GCAGACAGGA GGATGTGGGC 
TTCCGCGGAA ACAGCCCGGA AATGAGGGTT GAGCCACAGC CGAGGATGTG GGTGCCGGAG 
AGCCCTGTGT GTAGGTCGGG AAGAGGTGAG AGTAAGTCTG AGAGCTGGAG TGAGGAGCTG 
CAGCCTGGCC ACCCACGTGC CTGGCCTCCA TCCCCGGGCC GCTTTCGCGT GGAAGAAGGT 
GGCGGTGCAC CTTTCTGCTC AGCAGATGGA AGCACGAGTG CAGAGAAGAG ACACCTGGAG 
GTTAGCAACG GAATGGACGA GCTGGCAGGT AGCCCATTTC CTGTGACGAG AATGTCTTCA 
AGATCAAGTG ACGCAAAACC ACTGCCCGCG TCCTATCCAG CTGAACCTAG GGAGCCCCAG 
GAAAGTCCGA AAATCACCAG TGCTTTCAGC TCTGTGAAAC CAAGTGAAGC GGTCCCTCGG 
AAGTTTGACA GTGGTGGAGA GAGGGGGGCA GGGCTCCCAC TGTCCCTGTC TAACAAGAAC 
CGAGGGCTCT CAGCTCCAGA CTTACGGTCT GTGGGGCTCA CCCCTGGGCA AGAACAGGGT 
GCCAGTGAGC TAGAGGGGTC TTTGGGTGAA GCAAGCACAA TAGAAATCCC CCCAGGTGAG 
TCCTTGCAAG CCAGGGCTGC AAGGATCCTG GGCATTGAGG TGGCGGTGGA GTCCCTCCTG 
CCGGGCATCC GGAGAGCGGG ACAGAACCAG CCTGCTGAGC CCGATGCAAG TGCCTGCACC 
CCAGAGTCCC CCCAGGAAGA GTTGCTATCT CGCCCAGCAC CGGCAGATGT CCCCAGGGTG 
TCCACTGATG CCTTTTATGG CAGGAGGAAG TGCGGCTGGA CCAAGAGCCC TCTCTTTGTA 
GGGGACAGGG ACAGTGCCAG GCGGGCTCCT CAGGCTTTTG AGCACTCAGA TGTGGACGGG 
GTTGTCACCA GCACAGACCC TGTCCCTGAG CCTGAGCCCA GCCCCTTGGA GTCCAAGTTC 
TTCGAACAAA AGGATGTGGA AACAAAACCA CCCTTCAGGT CCACTTTATT CCATTTTGTA 
GAAAGAACCC CAAGTGTGGC AGGCTCTGAA AAGAGACTTA GAAGCCCTTC CAAAGTGATT 
GAAAGTTTAC AAGAGAAACT GGCCTCCCCG CCTAGGAGAG CAGACCCTGA CCGCCTGATG 
AGAATGAAAG AGGTGAGCTC AGTGTCACGG ATGAGAGTCC TGAGCTTCAG GAATGCCGAC 
TCCCAGGAGG ACGCCGAGGA ATTGAAGGCC ACCACAAGGG GCCAGGCCGG GCTCCCGGGA 
GGCCTTGTGT CTCCTGGCAG TGGGGACCGT GCCCAGAGAT TGGGCCACTC ACTCTCTGTG 
TCCAAGGACA GCATCTCCAG GGAAGAGAAG GAGCATCCGG CAGCACAAAA GGAGAAGAGC 
ATGGATCAAG ACTTCTGGTG CCCAGATTCC TATGACCCTA GCAGAGTGGA GAGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_001350001.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336930.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 2, mRNA.

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CloneID	OHu43833
Clone ID Related Accession (Same CDS sequence)	XM_011519609.2 , NM_001350001.1 , NM_001350021.1 , NM_001350001.2 , NM_001350021.2
Accession Version	NM_001350001.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158036.14 and AL050154.1. Transcript Variant: This variant (2) differs in the 5' UTR, contains an alternate exon in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.241189.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336930.1
CDS	619..4284
Translation	MAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_001350001.1
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350001.1

ORF Insert Sequence:

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_001350021.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336950.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu43833
Clone ID Related Accession (Same CDS sequence)	XM_011519609.2 , NM_001350001.1 , NM_001350021.1 , NM_001350001.2 , NM_001350021.2
Accession Version	NM_001350021.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360172.17, BX648192.1, AL158036.14 and AL050154.1. Transcript Variant: This variant (3) differs in the 5' UTR, contains an alternate exon in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX648192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336950.1
CDS	642..4307
Translation	MAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_001350021.1
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350021.1

ORF Insert Sequence:

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_001350001.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336930.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu43833
Clone ID Related Accession (Same CDS sequence)	XM_011519609.2 , NM_001350001.1 , NM_001350021.1 , NM_001350001.2 , NM_001350021.2
Accession Version	NM_001350001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL158036.14. On Jun 1, 2019 this sequence version replaced NM_001350001.1. Transcript Variant: This variant (2) differs in the 5' UTR, contains an alternate exon in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.241189.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336930.1
CDS	619..4284
Translation	MAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_001350001.2
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350001.2

ORF Insert Sequence:

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( NM_001350021.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336950.1 Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 3, mRNA.

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CloneID	OHu43833
Clone ID Related Accession (Same CDS sequence)	XM_011519609.2 , NM_001350001.1 , NM_001350021.1 , NM_001350001.2 , NM_001350021.2
Accession Version	NM_001350021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360172.17, BX648192.1 and AL158036.14. On May 31, 2019 this sequence version replaced NM_001350021.1. Transcript Variant: This variant (3) differs in the 5' UTR, contains an alternate exon in the 5' region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX648192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336950.1
CDS	642..4307
Translation	MAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	NM_001350021.2
Organism	Homo sapiens(human)
Definition	Homo sapiens junctional cadherin 5 associated (JCAD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001350021.2

ORF Insert Sequence:

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JCAD ( XM_011519609.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517911.1 Homo sapiens KIAA1462 (KIAA1462), transcript variant X2, mRNA.

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CloneID	OHu43833
Clone ID Related Accession (Same CDS sequence)	XM_011519609.2 , NM_001350001.1 , NM_001350021.1 , NM_001350001.2 , NM_001350021.2
Accession Version	XM_011519609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	junctional protein associated with coronary artery disease isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517911.1
CDS	619..4284
Translation	MAQAHSLPVHVREGPWEVGGRSEHVMKKPVWEEELRMSGPAKWQNVSLESWNQPRKLGRQMSDGDGERLFQDLYPFIQGEHVLNSQNKGKSRSLPRVLSPESLSCTEIPIPLNERHSPKMPPYPPTCAPNLDSTRNSEKSGCSAPFPRPKFGRPLKPPSYSSHQQSRGGADSSDSQDSQQMDAYVPRHELCLSDPGLEPPVYVPPPSYRSPPQNIPNPYLEDTVPINVCGGHSQQQSPTEKAGASGQPPSGPPGTGNEYGVSPRLPQGLPAHPRPVTAYDGFVQYIPFDDPRLRHFKLAQPQGFCEDIKLDDKSYNSSPVTAQEPAHGGMQPDGAIWNPQSLIPPSGDERGLVLADSSPRWLWGQPPGDGENSGLPNQRDRCVARGQWPDVRGSQHGHTGRQVSSPYSQGESTCETQTKLKKFQTGTRTKKSSKKKMNETIFCLVSIPVKSESHLPDRDMDNNDLKPSADQKNGSDKSPALQEQSLLSMSSTDLELQALTGSMGGRTEFQKQDLGEPEEDRQTNDLSFIHLTKHRELKHSGSWPGHRYRDQQTQTSFSEEPQSSQLLPGAKLGGPSRAALSPKCSDPAASEAQTHTAFPTGDHKQRPSARNLKGHRSLSPSSNSAFSRTSLSVDQAPTPKAGRSQPCVDVHGLGAHPGPKREVVKGEPTGPCNSKQLFGQFLLKPVSRRPWDLISQLESFNKELQEEEESSSSSSSSSSSSEESEAEPQQENRAHCRQEDVGFRGNSPEMRVEPQPRMWVPESPVCRSGRGESKSESWSEELQPGHPRAWPPSPGRFRVEEGGGAPFCSADGSTSAEKRHLEVSNGMDELAGSPFPVTRMSSRSSDAKPLPASYPAEPREPQESPKITSAFSSVKPSEAVPRKFDSGGERGAGLPLSLSNKNRGLSAPDLRSVGLTPGQEQGASELEGSLGEASTIEIPPGESLQARAARILGIEVAVESLLPGIRRAGQNQPAEPDASACTPESPQEELLSRPAPADVPRVSTDAFYGRRKCGWTKSPLFVGDRDSARRAPQAFEHSDVDGVVTSTDPVPEPEPSPLESKFFEQKDVETKPPFRSTLFHFVERTPSVAGSEKRLRSPSKVIESLQEKLASPPRRADPDRLMRMKEVSSVSRMRVLSFRNADSQEDAEELKATTRGQAGLPGGLVSPGSGDRAQRLGHSLSVSKDSISREEKEHPAAQKEKSMDQDFWCPDSYDPSRVERV

Target ORF information:

RefSeq Version	XM_011519609.2
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1462 (KIAA1462), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519609.2

ORF Insert Sequence:

ATGGCCCAAG CCCACAGCCT GCCTGTCCAC GTGAGGGAGG GTCCATGGGA AGTTGGAGGA 
AGGTCAGAGC ATGTGATGAA GAAGCCAGTT TGGGAAGAAG AATTGCGAAT GTCAGGTCCT 
GCCAAGTGGC AGAACGTCAG CCTGGAAAGC TGGAACCAGC CAAGGAAATT AGGGAGGCAG 
ATGTCTGATG GAGATGGGGA GAGACTGTTT CAAGACCTGT ACCCATTCAT TCAAGGAGAA 
CATGTGTTGA ATTCTCAAAA CAAAGGGAAG TCTCGCTCAC TGCCTAGAGT TCTTTCCCCC 
GAGAGCCTGA GTTGCACGGA AATTCCCATT CCATTAAATG AAAGACATTC ACCTAAAATG 
CCACCGTATC CTCCCACTTG CGCACCAAAT TTGGACTCCA CGAGGAATTC TGAGAAGAGT 
GGCTGCTCAG CCCCATTTCC CCGGCCTAAG TTTGGGAGGC CCCTCAAGCC CCCATCTTAC 
AGCTCGCACC AGCAGTCTAG GGGAGGAGCG GACAGCAGTG ACTCTCAGGA CAGCCAGCAG 
ATGGACGCCT ATGTCCCCAG GCATGAGCTC TGCCTGTCAG ACCCTGGATT GGAACCTCCA 
GTGTACGTGC CTCCGCCCTC ATACAGATCG CCCCCGCAGA ACATCCCAAA CCCCTACTTG 
GAAGACACGG TGCCCATAAA TGTGTGTGGC GGTCACAGTC AACAGCAGTC TCCGACCGAG 
AAGGCTGGGG CCAGCGGTCA GCCTCCTTCA GGCCCCCCTG GAACTGGGAA TGAGTATGGT 
GTGAGCCCCC GCTTGCCTCA GGGGCTCCCC GCACATCCCC GACCTGTCAC TGCCTATGAC 
GGCTTCGTTC AGTACATTCC CTTTGATGAT CCACGGTTAC GACATTTTAA ACTAGCTCAG 
CCCCAGGGTT TCTGTGAAGA CATAAAGCTT GACGATAAAT CATATAACTC CAGTCCTGTC 
ACTGCTCAAG AGCCGGCTCA TGGAGGAATG CAGCCTGATG GTGCCATTTG GAATCCACAG 
AGCTTAATAC CCCCGTCGGG GGATGAGAGA GGCCTGGTCT TGGCCGATTC CAGCCCCCGG 
TGGCTGTGGG GCCAGCCCCC CGGGGATGGG GAAAACAGTG GCCTCCCCAA CCAGAGAGAC 
CGCTGTGTGG CAAGGGGACA GTGGCCTGAT GTGAGAGGCA GCCAGCACGG GCACACTGGA 
AGACAAGTTT CCTCCCCTTA CTCACAGGGC GAGAGCACCT GCGAAACTCA AACCAAGCTC 
AAAAAGTTCC AAACTGGGAC TCGGACCAAG AAAAGTTCAA AGAAAAAAAT GAACGAGACT 
ATATTTTGCT TGGTTTCTAT CCCAGTGAAA TCAGAATCAC ATCTGCCAGA TAGAGATATG 
GACAACAATG ACTTAAAGCC CAGTGCTGAT CAAAAGAATG GGTCTGATAA GAGCCCGGCT 
CTGCAAGAAC AGAGTCTGCT GAGCATGTCT TCCACCGACC TGGAGCTGCA GGCCCTCACA 
GGAAGCATGG GTGGGAGAAC GGAGTTCCAA AAACAAGATC TAGGGGAACC AGAAGAAGAC 
AGACAAACAA ATGACCTCAG TTTCATCCAC CTTACAAAGC ACAGAGAACT CAAGCATTCT 
GGCTCTTGGC CAGGGCACCG GTACAGAGAT CAGCAAACAC AAACCAGTTT CTCCGAGGAG 
CCCCAAAGTT CGCAGCTGCT CCCTGGTGCA AAGCTGGGAG GGCCGAGTCG TGCAGCATTG 
AGTCCAAAAT GTTCAGACCC TGCTGCCTCC GAAGCTCAGA CGCACACAGC ATTCCCTACC 
GGTGATCACA AACAGAGGCC AAGTGCCCGT AACCTGAAAG GTCACAGGTC CCTCAGCCCA 
TCCAGCAACA GTGCGTTCTC AAGGACTTCC TTGTCCGTGG ACCAGGCACC GACGCCAAAA 
GCAGGCCGAA GTCAGCCCTG CGTGGATGTC CACGGGCTTG GAGCCCACCC TGGGCCTAAG 
CGGGAGGTGG TGAAGGGGGA GCCCACGGGC CCTTGCAACA GTAAACAACT CTTTGGGCAG 
TTTCTCCTGA AACCGGTCAG CCGTCGTCCC TGGGATTTGA TCAGTCAGTT AGAAAGTTTT 
AACAAGGAGC TCCAGGAAGA GGAAGAAAGC AGCAGTAGCA GCAGCAGCAG CAGCAGCAGC 
AGTGAGGAGA GTGAGGCGGA GCCGCAGCAG GAGAACCGTG CTCACTGCAG ACAGGAGGAT 
GTGGGCTTCC GCGGAAACAG CCCGGAAATG AGGGTTGAGC CACAGCCGAG GATGTGGGTG 
CCGGAGAGCC CTGTGTGTAG GTCGGGAAGA GGTGAGAGTA AGTCTGAGAG CTGGAGTGAG 
GAGCTGCAGC CTGGCCACCC ACGTGCCTGG CCTCCATCCC CGGGCCGCTT TCGCGTGGAA 
GAAGGTGGCG GTGCACCTTT CTGCTCAGCA GATGGAAGCA CGAGTGCAGA GAAGAGACAC 
CTGGAGGTTA GCAACGGAAT GGACGAGCTG GCAGGTAGCC CATTTCCTGT GACGAGAATG 
TCTTCAAGAT CAAGTGACGC AAAACCACTG CCCGCGTCCT ATCCAGCTGA ACCTAGGGAG 
CCCCAGGAAA GTCCGAAAAT CACCAGTGCT TTCAGCTCTG TGAAACCAAG TGAAGCGGTC 
CCTCGGAAGT TTGACAGTGG TGGAGAGAGG GGGGCAGGGC TCCCACTGTC CCTGTCTAAC 
AAGAACCGAG GGCTCTCAGC TCCAGACTTA CGGTCTGTGG GGCTCACCCC TGGGCAAGAA 
CAGGGTGCCA GTGAGCTAGA GGGGTCTTTG GGTGAAGCAA GCACAATAGA AATCCCCCCA 
GGTGAGTCCT TGCAAGCCAG GGCTGCAAGG ATCCTGGGCA TTGAGGTGGC GGTGGAGTCC 
CTCCTGCCGG GCATCCGGAG AGCGGGACAG AACCAGCCTG CTGAGCCCGA TGCAAGTGCC 
TGCACCCCAG AGTCCCCCCA GGAAGAGTTG CTATCTCGCC CAGCACCGGC AGATGTCCCC 
AGGGTGTCCA CTGATGCCTT TTATGGCAGG AGGAAGTGCG GCTGGACCAA GAGCCCTCTC 
TTTGTAGGGG ACAGGGACAG TGCCAGGCGG GCTCCTCAGG CTTTTGAGCA CTCAGATGTG 
GACGGGGTTG TCACCAGCAC AGACCCTGTC CCTGAGCCTG AGCCCAGCCC CTTGGAGTCC 
AAGTTCTTCG AACAAAAGGA TGTGGAAACA AAACCACCCT TCAGGTCCAC TTTATTCCAT 
TTTGTAGAAA GAACCCCAAG TGTGGCAGGC TCTGAAAAGA GACTTAGAAG CCCTTCCAAA 
GTGATTGAAA GTTTACAAGA GAAACTGGCC TCCCCGCCTA GGAGAGCAGA CCCTGACCGC 
CTGATGAGAA TGAAAGAGGT GAGCTCAGTG TCACGGATGA GAGTCCTGAG CTTCAGGAAT 
GCCGACTCCC AGGAGGACGC CGAGGAATTG AAGGCCACCA CAAGGGGCCA GGCCGGGCTC 
CCGGGAGGCC TTGTGTCTCC TGGCAGTGGG GACCGTGCCC AGAGATTGGG CCACTCACTC 
TCTGTGTCCA AGGACAGCAT CTCCAGGGAA GAGAAGGAGC ATCCGGCAGC ACAAAAGGAG 
AAGAGCATGG ATCAAGACTT CTGGTGCCCA GATTCCTATG ACCCTAGCAG AGTGGAGAGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	JCAD
Entrez Gene ID	57608
Full Name	junctional cadherin 5 associated
Synonyms	KIAA1462
General protein information	Preferred Names junctional cadherin 5 associated Names junctional protein associated with coronary artery disease
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p11.23
Summary	This gene encodes an endothelial cell-to-cell junction protein. Naturally occurring mutations in this gene are associated with coronary artery disease, late onset alzheimer disease, and emphysema distribution. [provided by RefSeq, Mar 2017].
Disorder MIM:	614398

Homo sapiens (human)	KIAA1462	NP_065899.1
Canis lupus familiaris (dog)	KIAA1462	XP_535151.2
	LOC694100	XP_001082542.1
Bos taurus (cattle)	KIAA1462	NP_001075943.1
Rattus norvegicus (Norway rat)	RGD1562037	XP_574050.3
Gallus gallus (chicken)	KIAA1462	XP_418578.3
Xenopus tropicalis (tropical clawed frog)	kiaa1462	XP_002940380.2
Mus musculus (house mouse)	9430020K01Rik	NP_001075432.1
Pan troglodytes (chimpanzee)	KIAA1462	XP_001136276.1

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JCAD cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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