ARHGAP21 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGAP21
Entrez Gene ID	57584
Full Name	Rho GTPase activating protein 21
Synonyms	ARHGAP10
General protein information	Preferred Names Rho GTPase activating protein 21 Names rho GTPase-activating protein 21 Rho-GTPase activating protein 10 rho GTPase-activating protein 10 rho-type GTPase-activating protein 21
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p12.1\|10p12.3
Summary	ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609870

mRNA and Protein(s)

mRNA	Protein	Name
XM_011519602.2	XP_011517904.1	rho GTPase-activating protein 21 isoform X1
XM_011519603.3	XP_011517905.1	rho GTPase-activating protein 21 isoform X1
NM_001367447.1	NP_001354376.1	rho GTPase-activating protein 21 isoform b
XM_011519603.2	XP_011517905.1	rho GTPase-activating protein 21 isoform X1
XM_005252542.3	XP_005252599.1	rho GTPase-activating protein 21 isoform X3
NM_001367449.1	NP_001354378.1	rho GTPase-activating protein 21 isoform c
XM_024448088.1	XP_024303856.1	rho GTPase-activating protein 21 isoform X6
NM_001367454.1	NP_001354383.1	rho GTPase-activating protein 21 isoform a
NM_001367453.1	NP_001354382.1	rho GTPase-activating protein 21 isoform b
XM_017016460.1	XP_016871949.1	rho GTPase-activating protein 21 isoform X4
XM_017016459.1	XP_016871948.1	rho GTPase-activating protein 21 isoform X3
NM_020824.3	NP_065875.3	rho GTPase-activating protein 21
NM_001367450.1	NP_001354379.1	rho GTPase-activating protein 21 isoform c
XM_011519605.2	XP_011517907.1	rho GTPase-activating protein 21 isoform X2
XM_017016459.2	XP_016871948.1	rho GTPase-activating protein 21 isoform X3
XM_011519606.2	XP_011517908.1	rho GTPase-activating protein 21 isoform X5
NM_001367448.1	NP_001354377.1	rho GTPase-activating protein 21 isoform a
XM_005252544.4	XP_005252601.1	rho GTPase-activating protein 21 isoform X4
NM_001367452.1	NP_001354381.1	rho GTPase-activating protein 21 isoform d
NM_001367455.1	NP_001354384.1	rho GTPase-activating protein 21 isoform e
XM_011519604.2	XP_011517906.1	rho GTPase-activating protein 21 isoform X1
XM_011519607.2	XP_011517909.1	rho GTPase-activating protein 21 isoform X7
NM_001367451.1	NP_001354380.1	rho GTPase-activating protein 21 isoform b
XM_017016461.1	XP_016871950.1	rho GTPase-activating protein 21 isoform X4
NM_020824.4	NP_065875.3	rho GTPase-activating protein 21 isoform a

Pathways from BioSystems

Homologies

	ARHGAP21	XP_001100413.2
Bos taurus (cattle)	ARHGAP21	XP_005214355.1
Gallus gallus (chicken)	ARHGAP21	NP_001170828.1
Canis lupus familiaris (dog)	ARHGAP21	XP_005617050.1
Mus musculus (house mouse)	Arhgap21	NP_001121556.2
Danio rerio (zebrafish)	arhgap21b	XP_005163349.1
Pan troglodytes (chimpanzee)	ARHGAP21	XP_507699.3
Xenopus tropicalis (tropical clawed frog)	arhgap21	NP_001090761.1
Homo sapiens (human)	ARHGAP21	NP_065875.3
Rattus norvegicus (Norway rat)	Arhgap21	NP_001178622.1

Gene Ontology
Bibliography

Related articles in PubMed

Hematopoietic defects in response to reduced Arhgap21.
Xavier-Ferrucio J, Ricon L, Vieira K, Longhini AL, Lazarini M, Bigarella CL, Franchi G Jr, Krause DS, Saad STO
Stem cell research2617-27(2017 Nov)

PTEN controls glandular morphogenesis through a juxtamembrane ?-Arrestin1/ARHGAP21 scaffolding complex.
Javadi A, Deevi RK, Evergren E, Blondel-Tepaz E, Baillie GS, Scott MG, Campbell FC
eLife6(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Genetic association of ARHGAP21 gene variant with mandibular prognathism.
Perillo L, Monsurr? A, Bonci E, Torella A, Mutarelli M, Nigro V
Journal of dental research94(4)569-76(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gly1121Ser variant in ARHGAP21 gene found to be shared by all MP individuals in arger branch of family with nearly complete penetrance. ARHGAP21 protein strengthens cell-cell adhesions; may be regulated by BMPs influencing mandibular growth.
Title: Genetic association of ARHGAP21 gene variant with mandibular prognathism.

ARHGAP21 is a Rho-GAP involved in cell-cell junction remodeling that affects migration and EMT through alpha-tubulin interaction and acetylation
Title: ARHGAP21 protein, a new partner of ?-tubulin involved in cell-cell adhesion formation and essential for epithelial-mesenchymal transition.

Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443.
Title: Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3.

silencing Cdc42 reduced influenza A virus replication, whereas silencing ARHGAP21 increased the virus replication.
Title: Transport of influenza virus neuraminidase (NA) to host cell surface is regulated by ARHGAP21 and Cdc42 proteins.

Disruption of the beta-arrestin 1/ARHGAP21 complex results in a more active ARHGAP21, leading to less efficient signaling via the angiotensin II type 1A receptor and, thereby, attenuation of stimulated stress fiber formation.
Title: ?-Arrestin 1 inhibits the GTPase-activating protein function of ARHGAP21, promoting activation of RhoA following angiotensin II type 1A receptor stimulation.

The following ARHGAP21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGAP21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu107687	NM_001367450.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu107687	NM_001367449.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu107722	NM_001367452.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu107755	NM_001367455.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu106538	XM_024448088.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu03540	NM_020824.4 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03540	NM_001367454.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03540	NM_001367448.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu43829	NM_001367451.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu43829	NM_001367447.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu43829	NM_001367453.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu03540	XM_005252542.3 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03540	XM_017016459.2 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu43829	XM_017016460.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu43829	XM_005252544.4 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu43829	XM_017016461.1 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70669	XM_011519604.2 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70669	XM_011519603.3 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70669	XM_011519602.2 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70670	XM_011519605.2 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70671	XM_011519606.2 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70672	XM_011519607.2 Latest version!	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu70669	XM_011519603.2	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu03540	XM_017016459.1	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu03540	NM_020824.3	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGAP21 ( NM_001367449.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354378.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu107687
Clone ID Related Accession (Same CDS sequence)	NM_001367449.1 , NM_001367450.1
Accession Version	NM_001367449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. Transcript Variant: This variant (7), as well as variant 8, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.258698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATACCA TATTTGTTAA GCAAGTTAAA GAAGGAGGAC CTGCTTTTGA AGCTGGATTA 
TGTACAGGTG ACCGAATTAT AAAAGTCAAT GGAGAAAGTG TTATTGGCAA AACCTATTCC 
CAAGTAATTG CTTTAATTCA AAACAGTGAT ACAACATTGG AACTTAGTGT TATGCCAAAA 
GATGAAGACA TTCTCCAAGT GCTACAGTTT ACAAAGGATG TCACAGCACT GGCATATTCT 
CAAGATGCCT ACCTGAAAGG CAACGAAGCT TATAGCGGCA ATGCCCGCAA TATACCTGAA 
CCTCCACCAA TCTGCTATCC CTGGCTGCCA TCTGCCCCAT CAGCCATGGC ACAGCCAGTT 
GAAATATCTC CTCCTGACTC ATCATTGAGC AAACAGCAAA CCAGTACACC AGTACTGACA 
CAACCTGGTA GGGCCTATAG AATGGAAATA CAAGTGCCTC CATCACCAAC AGATGTTGCA 
AAATCAAACA CAGCAGTGTG TGTTTGCAAT GAAAGTGTAA GGACTGTCAT TGTGCCTTCT 
GAGAAGGTTG TAGATTTGTT ATCCAATAGA AACAACCATA CAGGTCCTTC ACATAGAACT 
GAAGAAGTGA GGTATGGCGT GAGTGAGCAG ACCTCTTTAA AAACAGTGTC AAGAACCACA 
TCACCACCAT TATCAATTCC CACCACTCAT CTAATTCATC AGCCTGCAGG CTCCAGATCA 
CTGGAACCTT CTGGAATTTT ACTTAAGTCT GGAAATTACA GTGGACATTC TGATGGAATC 
TCAAGCAGCA GATCTCAAGC TGTGGAGGCT CCCTCTGTAT CTGTTAATCA CTATTCGCCA 
AATTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
AGACGATTGC ACATAGGTTG TCGGACAATA CAAGAAAGAT TAGATAGTTT AAGAGCAGCA 
TCTCAAAGCA CGACAGATTA TAACCAGGTC GTCCCCAACC GCACTACTTT GCAGGGACGA 
CGTCGAAGCA CCTCTCATGA TCGAGTGCCC CAGTCTGTCC AGATACGGCA ACGCAGTGTG 
TCCCAAGAAA GACTGGAAGA TTCTGTGCTA ATGAAGTATT GTCCAAGAAG TGCATCTCAA 
GGAGCACTGA CGTCTCCATC TGTTAGTTTT AGTAATCATA GAACTCGTTC ATGGGATTAT 
ATTGAGGGAC AGGATGAAAC CTTAGAAAAT GTCAATTCTG GAACTCCAAT ACCTGATTCC 
AATGGAGAGA AAAAACAGAC TTACAAGTGG AGTGGGTTTA CTGAACAGGA TGATAGACGA 
GGTATTTGTG AAAGACCTAG GCAGCAAGAA ATTCATAAAT CTTTTCGAGG TTCCAATTTT 
ACTGTGGCTC CAAGCGTTGT TAATTCTGAT AACAGGCGAA TGAGTGGTAG AGGAGTGGGA 
TCTGTGTCGC AGTTTAAAAA AATTCCACCA GATCTAAAAA CATTGCAGTC AAACAGAAAT 
TTTCAGACTA CTTGTGGAAT GTCACTGCCT CGGGGTATTT CACAAGACAG GTCACCTCTT 
GTGAAAGTCC GAAGTAATTC TCTGAAAGCT CCTTCCACGC ATGTCACAAA ACCATCATTT 
AGCCAGAAAT CATTTGTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAGAAC 
AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG ACTGACAGTG CCCCCGATCA GCAAGTGGAG 
ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA 
AACGCTGGTA CTTCAGATTT AGAACTACCT GTCAGTCAAA GGAATCAAGA TTTAAGTTTA 
CAAGAGGCTG AAACTGAGCA ATCAGATACT TTAGATAATA AAGAAGCTGT CATCCTAAGG 
GAAAAACCTC CATCTGGACG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTG 
GCAGTAAATG ACCAGGAGAC CGGGTCAGAC ACTACCTGCT GGCTGCCCAA TGATGCACGT 
CGAGAGGTCC ACATAAAAAG AATGGAGGAA AGAAAAGCCT CGAGTACCAG TCCGCCTGGC 
GATTCTTTGG CTTCCATCCC ATTTATAGAT GAACCAACTA GCCCTAGCAT TGATCATGAT 
ATTGCACATA TCCCTGCCTC TGCTGTTATA TCAGCCTCTA CCTCTCAGGT CCCCTCCATA 
GCAACAGTTC CTCCTTGCCT CACAACTTCA GCTCCATTAA TTCGCCGTCA GCTCTCACAT 
GACCACGAAT CTGTTGGCCC TCCTAGCCTG GATGCTCAGC CCAACTCAAA GACAGAAAGA 
TCAAAATCAT ATGATGAGGG TCTGGATGAT TACAGAGAAG ATGCAAAATT GTCCTTTAAG 
CACGTATCTA GTCTGAAGGG AATCAAGATC GCAGACAGCC AAAAGTCATC AGAAGACTCT 
GGGTCCAGAA AAGATTCTTC CTCAGAGGTC TTCAGTGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCCGAC CCCTTGTCAC CGATAAGGGC AAGCGAGTTG GTGGAAGTAT TCGGCCATGG 
AAACAGATGT ATGTTGTCCT TCGGGGTCAT TCACTTTACC TGTACAAAGA TAAAAGAGAG 
CAGACGACTC CGTCTGAGGA AGAGCAGCCC ATCAGTGTTA ATGCTTGCTT GATAGACATC 
TCTTACAGTG AGACCAAGAG GAAAAATGTG TTTCGACTCA CCACGTCCGA CTGTGAATGC 
CTGTTTCAGG CTGAAGACAG AGATGATATG CTAGCTTGGA TCAAGACGAT CCAGGAGAGC 
AGCAACCTAA ACGAAGAGGA CACTGGAGTC ACTAACAGGG ATCTAATTAG TCGAAGAATA 
AAAGAATACA ACAATCTGAT GAGCAAAGCA GAACAGTTGC CAAAAACACC TCGCCAGAGT 
CTCAGCATCA GGCAAACTTT GCTTGGTGCT AAATCAGAGC CAAAGACTCA AAGCCCACAC 
TCTCCGAAGG AAGAGTCGGA AAGGAAACTT CTCAGTAAAG ATGATACCAG TCCCCCAAAA 
GACAAAGGCA CATGGAGAAA AGGCATTCCA AGTATCATGA GAAAGACATT TGAGAAAAAG 
CCAACTGCTA CAGGAACTTT CGGCGTCCGA CTAGATGACT GCCCACCAGC TCATACTAAT 
CGGTATATTC CATTAATAGT TGACATATGT TGCAAATTAG TTGAAGAAAG AGGTCTTGAA 
TATACAGGTA TTTATAGAGT TCCTGGAAAT AATGCAGCCA TCTCAAGTAT GCAAGAAGAA 
CTCAACAAGG GAATGGCTGA TATTGATATA CAAGATGATA AATGGCGAGA TTTGAATGTG 
ATAAGCAGTT TACTAAAATC CTTCTTCAGA AAACTCCCTG AGCCTCTCTT CACAAATGAT 
AAATATGCTG ATTTTATTGA AGCCAATCGT AAAGAAGATC CTCTAGATCG TCTGAAAACA 
TTAAAAAGAC TAATTCACGA TTTGCCTGAA CATCATTATG AAACACTTAA GTTCCTTTCA 
GCTCATCTGA AGACAGTGGC AGAAAATTCA GAAAAAAATA AGATGGAACC AAGAAACCTA 
GCAATAGTGT TTGGTCCCAC CCTTGTTCGA ACATCAGAAG ACAACATGAC CCACATGGTC 
ACCCACATGC CTGACCAGTA CAAGATTGTA GAAACGCTCA TCCAGCACCA TGACTGGTTT 
TTCACAGAAG AAGGTGCTGA AGAGCCTCTT ACAACAGTGC AGGAGGAAAG CACAGTAGAC 
TCCCAGCCAG TGCCAAACAT AGATCATTTA CTCACCAACA TTGGAAGGAC AGGAGTCTCC 
CCAGGAGATG TATCAGATTC AGCTACTAGT GACTCAACAA AATCTAAGGG TTCTTGGGGA 
TCTGGAAAGG ATCAGTATAG CAGGGAACTG CTTGTGTCCT CCATCTTTGC AGCTGCTAGT 
CGCAAGAGGA AGAAGCCGAA AGAAAAAGCA CAGCCTAGCA GCTCAGAAGA TGAACTGGAC 
AATGTATTTT TTAAGAAAGA AAATGTGGAA CAGTGTCACA ATGATACTAA AGAGGAGTCC 
AAAAAAGAAA GTGAGACACT GGGCAGAAAA CAGAAGATCA TCATTGCCAA AGAAAACAGC 
ACTAGGAAAG ACCCCAGCAC GACAAAAGAT GAAAAGATAT CACTAGGAAA AGAGAGCACG 
CCTTCTGAAG AACCCTCACC ACCACACAAC TCAAAACACA ACAAGTCACC AACTCTCAGC 
TGTCGCTTTG CCATCCTGAA AGAGAGCCCC AGGTCACTTC TGGCACAGAA GTCCTCCCAC 
CTTGAAGAGA CAGGCTCTGA CTCTGGCACT TTGCTCAGCA CGTCTTCCCA GGCCTCCCTG 
GCAAGGTTTT CCATGAAGAA ATCAACCAGT CCAGAAACGA AACATAGCGA GTTTTTGGCC 
AACGTCAGCA CCATCACCTC AGATTATTCC ACCACATCGT CTGCTACATA CTTGACTAGC 
CTGGACTCCA GTCGACTGAG CCCTGAGGTG CAATCCGTGG CAGAGAGCAA GGGGGACGAG 
GCAGATGACG AGAGAAGCGA ACTCATCAGT GAAGGGCGGC CTGTGGAAAC CGACAGCGAG 
AGCGAGTTTC CCGTGTTCCC CACAGCCTTG ACTTCAGAGA GGCTTTTCCG AGGAAAACTG 
CAAGAAGTGA CTAAGAGCAG CCGGAGAAAT TCTGAAGGAA GTGAATTAAG TTGCACCGAG 
GGAAGTTTAA CATCAAGTTT AGATAGCCGG AGACAGCTCT TCAGTTCCCA TAAACTCATC 
GAATGTGATA CTCTTTCCAG GAAAAAATCA GCTAGATTCA AGTCAGATAG TGGAAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCACCT TCGTTAACTA AAGTGTTTGA TGTTATGAAA 
AAAGGAAAGT CAACTGGGAG TTTACTGACA CCCACCAGAG GCGAATCCGA AAAACAGGAA 
CCCACATGGA AAACGAAAAT AGCAGATCGG TTAAAACTGA GACCCAGAGC CCCTGCGGAT 
GACATGTTTG GAGTAGGGAA TCACAAAGTG AATGCCGAGA CTGCTAAAAG GAAAAGCATC 
CGGCGCAGAC ATACACTAGG AGGGCACAGA GATGCTACCG AAATCAGCGT TTTGAATTTT 
TGGAAAGTGC ATGAGCAGAG CGGGGAGAGA GAATCTGAAC TTTCAGCTGT AAACCGGTTA 
AAACCAAAAT GCTCAGCCCA GGACCTTTCC ATCTCAGACT GGCTGGCCAG GGAACGCCTA 
CGCACCAGTA CCTCTGACCT TAGCAGAGGA GAAATCGGAG ATCCCCAGAC AGAGAACCCA 
AGCACACGAG AAATAGCCAC GACCGACACA CCTTTGTCTC TTCATTGCAA CACAGGCAGT 
TCTTCCAGCA CCTTGGCTTC AACAAACAGG CCCCTTCTTT CCATACCACC ACAGTCACCT 
GACCAAATAA ACGGAGAAAG CTTCCAGAAC GTGAGCAAAA ATGCTAGTTC TGCAGCGAAT 
GCCCAACCTC ATAAACTGTC TGAAACCCCA GGCAGTAAAG CAGAGTTTCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354378.1
CDS	786..6368
Translation	MDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367449.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367449.1

ORF Insert Sequence:

ATGGATACCA TATTTGTTAA GCAAGTTAAA GAAGGAGGAC CTGCTTTTGA AGCTGGATTA 
TGTACAGGTG ACCGAATTAT AAAAGTCAAT GGAGAAAGTG TTATTGGCAA AACCTATTCC 
CAAGTAATTG CTTTAATTCA AAACAGTGAT ACAACATTGG AACTTAGTGT TATGCCAAAA 
GATGAAGACA TTCTCCAAGT GCTACAGTTT ACAAAGGATG TCACAGCACT GGCATATTCT 
CAAGATGCCT ACCTGAAAGG CAACGAAGCT TATAGCGGCA ATGCCCGCAA TATACCTGAA 
CCTCCACCAA TCTGCTATCC CTGGCTGCCA TCTGCCCCAT CAGCCATGGC ACAGCCAGTT 
GAAATATCTC CTCCTGACTC ATCATTGAGC AAACAGCAAA CCAGTACACC AGTACTGACA 
CAACCTGGTA GGGCCTATAG AATGGAAATA CAAGTGCCTC CATCACCAAC AGATGTTGCA 
AAATCAAACA CAGCAGTGTG TGTTTGCAAT GAAAGTGTAA GGACTGTCAT TGTGCCTTCT 
GAGAAGGTTG TAGATTTGTT ATCCAATAGA AACAACCATA CAGGTCCTTC ACATAGAACT 
GAAGAAGTGA GGTATGGCGT GAGTGAGCAG ACCTCTTTAA AAACAGTGTC AAGAACCACA 
TCACCACCAT TATCAATTCC CACCACTCAT CTAATTCATC AGCCTGCAGG CTCCAGATCA 
CTGGAACCTT CTGGAATTTT ACTTAAGTCT GGAAATTACA GTGGACATTC TGATGGAATC 
TCAAGCAGCA GATCTCAAGC TGTGGAGGCT CCCTCTGTAT CTGTTAATCA CTATTCGCCA 
AATTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
AGACGATTGC ACATAGGTTG TCGGACAATA CAAGAAAGAT TAGATAGTTT AAGAGCAGCA 
TCTCAAAGCA CGACAGATTA TAACCAGGTC GTCCCCAACC GCACTACTTT GCAGGGACGA 
CGTCGAAGCA CCTCTCATGA TCGAGTGCCC CAGTCTGTCC AGATACGGCA ACGCAGTGTG 
TCCCAAGAAA GACTGGAAGA TTCTGTGCTA ATGAAGTATT GTCCAAGAAG TGCATCTCAA 
GGAGCACTGA CGTCTCCATC TGTTAGTTTT AGTAATCATA GAACTCGTTC ATGGGATTAT 
ATTGAGGGAC AGGATGAAAC CTTAGAAAAT GTCAATTCTG GAACTCCAAT ACCTGATTCC 
AATGGAGAGA AAAAACAGAC TTACAAGTGG AGTGGGTTTA CTGAACAGGA TGATAGACGA 
GGTATTTGTG AAAGACCTAG GCAGCAAGAA ATTCATAAAT CTTTTCGAGG TTCCAATTTT 
ACTGTGGCTC CAAGCGTTGT TAATTCTGAT AACAGGCGAA TGAGTGGTAG AGGAGTGGGA 
TCTGTGTCGC AGTTTAAAAA AATTCCACCA GATCTAAAAA CATTGCAGTC AAACAGAAAT 
TTTCAGACTA CTTGTGGAAT GTCACTGCCT CGGGGTATTT CACAAGACAG GTCACCTCTT 
GTGAAAGTCC GAAGTAATTC TCTGAAAGCT CCTTCCACGC ATGTCACAAA ACCATCATTT 
AGCCAGAAAT CATTTGTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAGAAC 
AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG ACTGACAGTG CCCCCGATCA GCAAGTGGAG 
ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA 
AACGCTGGTA CTTCAGATTT AGAACTACCT GTCAGTCAAA GGAATCAAGA TTTAAGTTTA 
CAAGAGGCTG AAACTGAGCA ATCAGATACT TTAGATAATA AAGAAGCTGT CATCCTAAGG 
GAAAAACCTC CATCTGGACG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTG 
GCAGTAAATG ACCAGGAGAC CGGGTCAGAC ACTACCTGCT GGCTGCCCAA TGATGCACGT 
CGAGAGGTCC ACATAAAAAG AATGGAGGAA AGAAAAGCCT CGAGTACCAG TCCGCCTGGC 
GATTCTTTGG CTTCCATCCC ATTTATAGAT GAACCAACTA GCCCTAGCAT TGATCATGAT 
ATTGCACATA TCCCTGCCTC TGCTGTTATA TCAGCCTCTA CCTCTCAGGT CCCCTCCATA 
GCAACAGTTC CTCCTTGCCT CACAACTTCA GCTCCATTAA TTCGCCGTCA GCTCTCACAT 
GACCACGAAT CTGTTGGCCC TCCTAGCCTG GATGCTCAGC CCAACTCAAA GACAGAAAGA 
TCAAAATCAT ATGATGAGGG TCTGGATGAT TACAGAGAAG ATGCAAAATT GTCCTTTAAG 
CACGTATCTA GTCTGAAGGG AATCAAGATC GCAGACAGCC AAAAGTCATC AGAAGACTCT 
GGGTCCAGAA AAGATTCTTC CTCAGAGGTC TTCAGTGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCCGAC CCCTTGTCAC CGATAAGGGC AAGCGAGTTG GTGGAAGTAT TCGGCCATGG 
AAACAGATGT ATGTTGTCCT TCGGGGTCAT TCACTTTACC TGTACAAAGA TAAAAGAGAG 
CAGACGACTC CGTCTGAGGA AGAGCAGCCC ATCAGTGTTA ATGCTTGCTT GATAGACATC 
TCTTACAGTG AGACCAAGAG GAAAAATGTG TTTCGACTCA CCACGTCCGA CTGTGAATGC 
CTGTTTCAGG CTGAAGACAG AGATGATATG CTAGCTTGGA TCAAGACGAT CCAGGAGAGC 
AGCAACCTAA ACGAAGAGGA CACTGGAGTC ACTAACAGGG ATCTAATTAG TCGAAGAATA 
AAAGAATACA ACAATCTGAT GAGCAAAGCA GAACAGTTGC CAAAAACACC TCGCCAGAGT 
CTCAGCATCA GGCAAACTTT GCTTGGTGCT AAATCAGAGC CAAAGACTCA AAGCCCACAC 
TCTCCGAAGG AAGAGTCGGA AAGGAAACTT CTCAGTAAAG ATGATACCAG TCCCCCAAAA 
GACAAAGGCA CATGGAGAAA AGGCATTCCA AGTATCATGA GAAAGACATT TGAGAAAAAG 
CCAACTGCTA CAGGAACTTT CGGCGTCCGA CTAGATGACT GCCCACCAGC TCATACTAAT 
CGGTATATTC CATTAATAGT TGACATATGT TGCAAATTAG TTGAAGAAAG AGGTCTTGAA 
TATACAGGTA TTTATAGAGT TCCTGGAAAT AATGCAGCCA TCTCAAGTAT GCAAGAAGAA 
CTCAACAAGG GAATGGCTGA TATTGATATA CAAGATGATA AATGGCGAGA TTTGAATGTG 
ATAAGCAGTT TACTAAAATC CTTCTTCAGA AAACTCCCTG AGCCTCTCTT CACAAATGAT 
AAATATGCTG ATTTTATTGA AGCCAATCGT AAAGAAGATC CTCTAGATCG TCTGAAAACA 
TTAAAAAGAC TAATTCACGA TTTGCCTGAA CATCATTATG AAACACTTAA GTTCCTTTCA 
GCTCATCTGA AGACAGTGGC AGAAAATTCA GAAAAAAATA AGATGGAACC AAGAAACCTA 
GCAATAGTGT TTGGTCCCAC CCTTGTTCGA ACATCAGAAG ACAACATGAC CCACATGGTC 
ACCCACATGC CTGACCAGTA CAAGATTGTA GAAACGCTCA TCCAGCACCA TGACTGGTTT 
TTCACAGAAG AAGGTGCTGA AGAGCCTCTT ACAACAGTGC AGGAGGAAAG CACAGTAGAC 
TCCCAGCCAG TGCCAAACAT AGATCATTTA CTCACCAACA TTGGAAGGAC AGGAGTCTCC 
CCAGGAGATG TATCAGATTC AGCTACTAGT GACTCAACAA AATCTAAGGG TTCTTGGGGA 
TCTGGAAAGG ATCAGTATAG CAGGGAACTG CTTGTGTCCT CCATCTTTGC AGCTGCTAGT 
CGCAAGAGGA AGAAGCCGAA AGAAAAAGCA CAGCCTAGCA GCTCAGAAGA TGAACTGGAC 
AATGTATTTT TTAAGAAAGA AAATGTGGAA CAGTGTCACA ATGATACTAA AGAGGAGTCC 
AAAAAAGAAA GTGAGACACT GGGCAGAAAA CAGAAGATCA TCATTGCCAA AGAAAACAGC 
ACTAGGAAAG ACCCCAGCAC GACAAAAGAT GAAAAGATAT CACTAGGAAA AGAGAGCACG 
CCTTCTGAAG AACCCTCACC ACCACACAAC TCAAAACACA ACAAGTCACC AACTCTCAGC 
TGTCGCTTTG CCATCCTGAA AGAGAGCCCC AGGTCACTTC TGGCACAGAA GTCCTCCCAC 
CTTGAAGAGA CAGGCTCTGA CTCTGGCACT TTGCTCAGCA CGTCTTCCCA GGCCTCCCTG 
GCAAGGTTTT CCATGAAGAA ATCAACCAGT CCAGAAACGA AACATAGCGA GTTTTTGGCC 
AACGTCAGCA CCATCACCTC AGATTATTCC ACCACATCGT CTGCTACATA CTTGACTAGC 
CTGGACTCCA GTCGACTGAG CCCTGAGGTG CAATCCGTGG CAGAGAGCAA GGGGGACGAG 
GCAGATGACG AGAGAAGCGA ACTCATCAGT GAAGGGCGGC CTGTGGAAAC CGACAGCGAG 
AGCGAGTTTC CCGTGTTCCC CACAGCCTTG ACTTCAGAGA GGCTTTTCCG AGGAAAACTG 
CAAGAAGTGA CTAAGAGCAG CCGGAGAAAT TCTGAAGGAA GTGAATTAAG TTGCACCGAG 
GGAAGTTTAA CATCAAGTTT AGATAGCCGG AGACAGCTCT TCAGTTCCCA TAAACTCATC 
GAATGTGATA CTCTTTCCAG GAAAAAATCA GCTAGATTCA AGTCAGATAG TGGAAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCACCT TCGTTAACTA AAGTGTTTGA TGTTATGAAA 
AAAGGAAAGT CAACTGGGAG TTTACTGACA CCCACCAGAG GCGAATCCGA AAAACAGGAA 
CCCACATGGA AAACGAAAAT AGCAGATCGG TTAAAACTGA GACCCAGAGC CCCTGCGGAT 
GACATGTTTG GAGTAGGGAA TCACAAAGTG AATGCCGAGA CTGCTAAAAG GAAAAGCATC 
CGGCGCAGAC ATACACTAGG AGGGCACAGA GATGCTACCG AAATCAGCGT TTTGAATTTT 
TGGAAAGTGC ATGAGCAGAG CGGGGAGAGA GAATCTGAAC TTTCAGCTGT AAACCGGTTA 
AAACCAAAAT GCTCAGCCCA GGACCTTTCC ATCTCAGACT GGCTGGCCAG GGAACGCCTA 
CGCACCAGTA CCTCTGACCT TAGCAGAGGA GAAATCGGAG ATCCCCAGAC AGAGAACCCA 
AGCACACGAG AAATAGCCAC GACCGACACA CCTTTGTCTC TTCATTGCAA CACAGGCAGT 
TCTTCCAGCA CCTTGGCTTC AACAAACAGG CCCCTTCTTT CCATACCACC ACAGTCACCT 
GACCAAATAA ACGGAGAAAG CTTCCAGAAC GTGAGCAAAA ATGCTAGTTC TGCAGCGAAT 
GCCCAACCTC ATAAACTGTC TGAAACCCCA GGCAGTAAAG CAGAGTTTCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367450.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354379.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 8, mRNA.

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CloneID	OHu107687
Clone ID Related Accession (Same CDS sequence)	NM_001367449.1 , NM_001367450.1
Accession Version	NM_001367450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. Transcript Variant: This variant (8), as well as variant 7, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155227.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATACCA TATTTGTTAA GCAAGTTAAA GAAGGAGGAC CTGCTTTTGA AGCTGGATTA 
TGTACAGGTG ACCGAATTAT AAAAGTCAAT GGAGAAAGTG TTATTGGCAA AACCTATTCC 
CAAGTAATTG CTTTAATTCA AAACAGTGAT ACAACATTGG AACTTAGTGT TATGCCAAAA 
GATGAAGACA TTCTCCAAGT GCTACAGTTT ACAAAGGATG TCACAGCACT GGCATATTCT 
CAAGATGCCT ACCTGAAAGG CAACGAAGCT TATAGCGGCA ATGCCCGCAA TATACCTGAA 
CCTCCACCAA TCTGCTATCC CTGGCTGCCA TCTGCCCCAT CAGCCATGGC ACAGCCAGTT 
GAAATATCTC CTCCTGACTC ATCATTGAGC AAACAGCAAA CCAGTACACC AGTACTGACA 
CAACCTGGTA GGGCCTATAG AATGGAAATA CAAGTGCCTC CATCACCAAC AGATGTTGCA 
AAATCAAACA CAGCAGTGTG TGTTTGCAAT GAAAGTGTAA GGACTGTCAT TGTGCCTTCT 
GAGAAGGTTG TAGATTTGTT ATCCAATAGA AACAACCATA CAGGTCCTTC ACATAGAACT 
GAAGAAGTGA GGTATGGCGT GAGTGAGCAG ACCTCTTTAA AAACAGTGTC AAGAACCACA 
TCACCACCAT TATCAATTCC CACCACTCAT CTAATTCATC AGCCTGCAGG CTCCAGATCA 
CTGGAACCTT CTGGAATTTT ACTTAAGTCT GGAAATTACA GTGGACATTC TGATGGAATC 
TCAAGCAGCA GATCTCAAGC TGTGGAGGCT CCCTCTGTAT CTGTTAATCA CTATTCGCCA 
AATTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
AGACGATTGC ACATAGGTTG TCGGACAATA CAAGAAAGAT TAGATAGTTT AAGAGCAGCA 
TCTCAAAGCA CGACAGATTA TAACCAGGTC GTCCCCAACC GCACTACTTT GCAGGGACGA 
CGTCGAAGCA CCTCTCATGA TCGAGTGCCC CAGTCTGTCC AGATACGGCA ACGCAGTGTG 
TCCCAAGAAA GACTGGAAGA TTCTGTGCTA ATGAAGTATT GTCCAAGAAG TGCATCTCAA 
GGAGCACTGA CGTCTCCATC TGTTAGTTTT AGTAATCATA GAACTCGTTC ATGGGATTAT 
ATTGAGGGAC AGGATGAAAC CTTAGAAAAT GTCAATTCTG GAACTCCAAT ACCTGATTCC 
AATGGAGAGA AAAAACAGAC TTACAAGTGG AGTGGGTTTA CTGAACAGGA TGATAGACGA 
GGTATTTGTG AAAGACCTAG GCAGCAAGAA ATTCATAAAT CTTTTCGAGG TTCCAATTTT 
ACTGTGGCTC CAAGCGTTGT TAATTCTGAT AACAGGCGAA TGAGTGGTAG AGGAGTGGGA 
TCTGTGTCGC AGTTTAAAAA AATTCCACCA GATCTAAAAA CATTGCAGTC AAACAGAAAT 
TTTCAGACTA CTTGTGGAAT GTCACTGCCT CGGGGTATTT CACAAGACAG GTCACCTCTT 
GTGAAAGTCC GAAGTAATTC TCTGAAAGCT CCTTCCACGC ATGTCACAAA ACCATCATTT 
AGCCAGAAAT CATTTGTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAGAAC 
AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG ACTGACAGTG CCCCCGATCA GCAAGTGGAG 
ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA 
AACGCTGGTA CTTCAGATTT AGAACTACCT GTCAGTCAAA GGAATCAAGA TTTAAGTTTA 
CAAGAGGCTG AAACTGAGCA ATCAGATACT TTAGATAATA AAGAAGCTGT CATCCTAAGG 
GAAAAACCTC CATCTGGACG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTG 
GCAGTAAATG ACCAGGAGAC CGGGTCAGAC ACTACCTGCT GGCTGCCCAA TGATGCACGT 
CGAGAGGTCC ACATAAAAAG AATGGAGGAA AGAAAAGCCT CGAGTACCAG TCCGCCTGGC 
GATTCTTTGG CTTCCATCCC ATTTATAGAT GAACCAACTA GCCCTAGCAT TGATCATGAT 
ATTGCACATA TCCCTGCCTC TGCTGTTATA TCAGCCTCTA CCTCTCAGGT CCCCTCCATA 
GCAACAGTTC CTCCTTGCCT CACAACTTCA GCTCCATTAA TTCGCCGTCA GCTCTCACAT 
GACCACGAAT CTGTTGGCCC TCCTAGCCTG GATGCTCAGC CCAACTCAAA GACAGAAAGA 
TCAAAATCAT ATGATGAGGG TCTGGATGAT TACAGAGAAG ATGCAAAATT GTCCTTTAAG 
CACGTATCTA GTCTGAAGGG AATCAAGATC GCAGACAGCC AAAAGTCATC AGAAGACTCT 
GGGTCCAGAA AAGATTCTTC CTCAGAGGTC TTCAGTGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCCGAC CCCTTGTCAC CGATAAGGGC AAGCGAGTTG GTGGAAGTAT TCGGCCATGG 
AAACAGATGT ATGTTGTCCT TCGGGGTCAT TCACTTTACC TGTACAAAGA TAAAAGAGAG 
CAGACGACTC CGTCTGAGGA AGAGCAGCCC ATCAGTGTTA ATGCTTGCTT GATAGACATC 
TCTTACAGTG AGACCAAGAG GAAAAATGTG TTTCGACTCA CCACGTCCGA CTGTGAATGC 
CTGTTTCAGG CTGAAGACAG AGATGATATG CTAGCTTGGA TCAAGACGAT CCAGGAGAGC 
AGCAACCTAA ACGAAGAGGA CACTGGAGTC ACTAACAGGG ATCTAATTAG TCGAAGAATA 
AAAGAATACA ACAATCTGAT GAGCAAAGCA GAACAGTTGC CAAAAACACC TCGCCAGAGT 
CTCAGCATCA GGCAAACTTT GCTTGGTGCT AAATCAGAGC CAAAGACTCA AAGCCCACAC 
TCTCCGAAGG AAGAGTCGGA AAGGAAACTT CTCAGTAAAG ATGATACCAG TCCCCCAAAA 
GACAAAGGCA CATGGAGAAA AGGCATTCCA AGTATCATGA GAAAGACATT TGAGAAAAAG 
CCAACTGCTA CAGGAACTTT CGGCGTCCGA CTAGATGACT GCCCACCAGC TCATACTAAT 
CGGTATATTC CATTAATAGT TGACATATGT TGCAAATTAG TTGAAGAAAG AGGTCTTGAA 
TATACAGGTA TTTATAGAGT TCCTGGAAAT AATGCAGCCA TCTCAAGTAT GCAAGAAGAA 
CTCAACAAGG GAATGGCTGA TATTGATATA CAAGATGATA AATGGCGAGA TTTGAATGTG 
ATAAGCAGTT TACTAAAATC CTTCTTCAGA AAACTCCCTG AGCCTCTCTT CACAAATGAT 
AAATATGCTG ATTTTATTGA AGCCAATCGT AAAGAAGATC CTCTAGATCG TCTGAAAACA 
TTAAAAAGAC TAATTCACGA TTTGCCTGAA CATCATTATG AAACACTTAA GTTCCTTTCA 
GCTCATCTGA AGACAGTGGC AGAAAATTCA GAAAAAAATA AGATGGAACC AAGAAACCTA 
GCAATAGTGT TTGGTCCCAC CCTTGTTCGA ACATCAGAAG ACAACATGAC CCACATGGTC 
ACCCACATGC CTGACCAGTA CAAGATTGTA GAAACGCTCA TCCAGCACCA TGACTGGTTT 
TTCACAGAAG AAGGTGCTGA AGAGCCTCTT ACAACAGTGC AGGAGGAAAG CACAGTAGAC 
TCCCAGCCAG TGCCAAACAT AGATCATTTA CTCACCAACA TTGGAAGGAC AGGAGTCTCC 
CCAGGAGATG TATCAGATTC AGCTACTAGT GACTCAACAA AATCTAAGGG TTCTTGGGGA 
TCTGGAAAGG ATCAGTATAG CAGGGAACTG CTTGTGTCCT CCATCTTTGC AGCTGCTAGT 
CGCAAGAGGA AGAAGCCGAA AGAAAAAGCA CAGCCTAGCA GCTCAGAAGA TGAACTGGAC 
AATGTATTTT TTAAGAAAGA AAATGTGGAA CAGTGTCACA ATGATACTAA AGAGGAGTCC 
AAAAAAGAAA GTGAGACACT GGGCAGAAAA CAGAAGATCA TCATTGCCAA AGAAAACAGC 
ACTAGGAAAG ACCCCAGCAC GACAAAAGAT GAAAAGATAT CACTAGGAAA AGAGAGCACG 
CCTTCTGAAG AACCCTCACC ACCACACAAC TCAAAACACA ACAAGTCACC AACTCTCAGC 
TGTCGCTTTG CCATCCTGAA AGAGAGCCCC AGGTCACTTC TGGCACAGAA GTCCTCCCAC 
CTTGAAGAGA CAGGCTCTGA CTCTGGCACT TTGCTCAGCA CGTCTTCCCA GGCCTCCCTG 
GCAAGGTTTT CCATGAAGAA ATCAACCAGT CCAGAAACGA AACATAGCGA GTTTTTGGCC 
AACGTCAGCA CCATCACCTC AGATTATTCC ACCACATCGT CTGCTACATA CTTGACTAGC 
CTGGACTCCA GTCGACTGAG CCCTGAGGTG CAATCCGTGG CAGAGAGCAA GGGGGACGAG 
GCAGATGACG AGAGAAGCGA ACTCATCAGT GAAGGGCGGC CTGTGGAAAC CGACAGCGAG 
AGCGAGTTTC CCGTGTTCCC CACAGCCTTG ACTTCAGAGA GGCTTTTCCG AGGAAAACTG 
CAAGAAGTGA CTAAGAGCAG CCGGAGAAAT TCTGAAGGAA GTGAATTAAG TTGCACCGAG 
GGAAGTTTAA CATCAAGTTT AGATAGCCGG AGACAGCTCT TCAGTTCCCA TAAACTCATC 
GAATGTGATA CTCTTTCCAG GAAAAAATCA GCTAGATTCA AGTCAGATAG TGGAAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCACCT TCGTTAACTA AAGTGTTTGA TGTTATGAAA 
AAAGGAAAGT CAACTGGGAG TTTACTGACA CCCACCAGAG GCGAATCCGA AAAACAGGAA 
CCCACATGGA AAACGAAAAT AGCAGATCGG TTAAAACTGA GACCCAGAGC CCCTGCGGAT 
GACATGTTTG GAGTAGGGAA TCACAAAGTG AATGCCGAGA CTGCTAAAAG GAAAAGCATC 
CGGCGCAGAC ATACACTAGG AGGGCACAGA GATGCTACCG AAATCAGCGT TTTGAATTTT 
TGGAAAGTGC ATGAGCAGAG CGGGGAGAGA GAATCTGAAC TTTCAGCTGT AAACCGGTTA 
AAACCAAAAT GCTCAGCCCA GGACCTTTCC ATCTCAGACT GGCTGGCCAG GGAACGCCTA 
CGCACCAGTA CCTCTGACCT TAGCAGAGGA GAAATCGGAG ATCCCCAGAC AGAGAACCCA 
AGCACACGAG AAATAGCCAC GACCGACACA CCTTTGTCTC TTCATTGCAA CACAGGCAGT 
TCTTCCAGCA CCTTGGCTTC AACAAACAGG CCCCTTCTTT CCATACCACC ACAGTCACCT 
GACCAAATAA ACGGAGAAAG CTTCCAGAAC GTGAGCAAAA ATGCTAGTTC TGCAGCGAAT 
GCCCAACCTC ATAAACTGTC TGAAACCCCA GGCAGTAAAG CAGAGTTTCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354379.1
CDS	1111..6693
Translation	MDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367450.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367450.1

ORF Insert Sequence:

ATGGATACCA TATTTGTTAA GCAAGTTAAA GAAGGAGGAC CTGCTTTTGA AGCTGGATTA 
TGTACAGGTG ACCGAATTAT AAAAGTCAAT GGAGAAAGTG TTATTGGCAA AACCTATTCC 
CAAGTAATTG CTTTAATTCA AAACAGTGAT ACAACATTGG AACTTAGTGT TATGCCAAAA 
GATGAAGACA TTCTCCAAGT GCTACAGTTT ACAAAGGATG TCACAGCACT GGCATATTCT 
CAAGATGCCT ACCTGAAAGG CAACGAAGCT TATAGCGGCA ATGCCCGCAA TATACCTGAA 
CCTCCACCAA TCTGCTATCC CTGGCTGCCA TCTGCCCCAT CAGCCATGGC ACAGCCAGTT 
GAAATATCTC CTCCTGACTC ATCATTGAGC AAACAGCAAA CCAGTACACC AGTACTGACA 
CAACCTGGTA GGGCCTATAG AATGGAAATA CAAGTGCCTC CATCACCAAC AGATGTTGCA 
AAATCAAACA CAGCAGTGTG TGTTTGCAAT GAAAGTGTAA GGACTGTCAT TGTGCCTTCT 
GAGAAGGTTG TAGATTTGTT ATCCAATAGA AACAACCATA CAGGTCCTTC ACATAGAACT 
GAAGAAGTGA GGTATGGCGT GAGTGAGCAG ACCTCTTTAA AAACAGTGTC AAGAACCACA 
TCACCACCAT TATCAATTCC CACCACTCAT CTAATTCATC AGCCTGCAGG CTCCAGATCA 
CTGGAACCTT CTGGAATTTT ACTTAAGTCT GGAAATTACA GTGGACATTC TGATGGAATC 
TCAAGCAGCA GATCTCAAGC TGTGGAGGCT CCCTCTGTAT CTGTTAATCA CTATTCGCCA 
AATTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
AGACGATTGC ACATAGGTTG TCGGACAATA CAAGAAAGAT TAGATAGTTT AAGAGCAGCA 
TCTCAAAGCA CGACAGATTA TAACCAGGTC GTCCCCAACC GCACTACTTT GCAGGGACGA 
CGTCGAAGCA CCTCTCATGA TCGAGTGCCC CAGTCTGTCC AGATACGGCA ACGCAGTGTG 
TCCCAAGAAA GACTGGAAGA TTCTGTGCTA ATGAAGTATT GTCCAAGAAG TGCATCTCAA 
GGAGCACTGA CGTCTCCATC TGTTAGTTTT AGTAATCATA GAACTCGTTC ATGGGATTAT 
ATTGAGGGAC AGGATGAAAC CTTAGAAAAT GTCAATTCTG GAACTCCAAT ACCTGATTCC 
AATGGAGAGA AAAAACAGAC TTACAAGTGG AGTGGGTTTA CTGAACAGGA TGATAGACGA 
GGTATTTGTG AAAGACCTAG GCAGCAAGAA ATTCATAAAT CTTTTCGAGG TTCCAATTTT 
ACTGTGGCTC CAAGCGTTGT TAATTCTGAT AACAGGCGAA TGAGTGGTAG AGGAGTGGGA 
TCTGTGTCGC AGTTTAAAAA AATTCCACCA GATCTAAAAA CATTGCAGTC AAACAGAAAT 
TTTCAGACTA CTTGTGGAAT GTCACTGCCT CGGGGTATTT CACAAGACAG GTCACCTCTT 
GTGAAAGTCC GAAGTAATTC TCTGAAAGCT CCTTCCACGC ATGTCACAAA ACCATCATTT 
AGCCAGAAAT CATTTGTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAGAAC 
AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG ACTGACAGTG CCCCCGATCA GCAAGTGGAG 
ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA 
AACGCTGGTA CTTCAGATTT AGAACTACCT GTCAGTCAAA GGAATCAAGA TTTAAGTTTA 
CAAGAGGCTG AAACTGAGCA ATCAGATACT TTAGATAATA AAGAAGCTGT CATCCTAAGG 
GAAAAACCTC CATCTGGACG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTG 
GCAGTAAATG ACCAGGAGAC CGGGTCAGAC ACTACCTGCT GGCTGCCCAA TGATGCACGT 
CGAGAGGTCC ACATAAAAAG AATGGAGGAA AGAAAAGCCT CGAGTACCAG TCCGCCTGGC 
GATTCTTTGG CTTCCATCCC ATTTATAGAT GAACCAACTA GCCCTAGCAT TGATCATGAT 
ATTGCACATA TCCCTGCCTC TGCTGTTATA TCAGCCTCTA CCTCTCAGGT CCCCTCCATA 
GCAACAGTTC CTCCTTGCCT CACAACTTCA GCTCCATTAA TTCGCCGTCA GCTCTCACAT 
GACCACGAAT CTGTTGGCCC TCCTAGCCTG GATGCTCAGC CCAACTCAAA GACAGAAAGA 
TCAAAATCAT ATGATGAGGG TCTGGATGAT TACAGAGAAG ATGCAAAATT GTCCTTTAAG 
CACGTATCTA GTCTGAAGGG AATCAAGATC GCAGACAGCC AAAAGTCATC AGAAGACTCT 
GGGTCCAGAA AAGATTCTTC CTCAGAGGTC TTCAGTGATG CTGCCAAGGA AGGGTGGCTT 
CATTTCCGAC CCCTTGTCAC CGATAAGGGC AAGCGAGTTG GTGGAAGTAT TCGGCCATGG 
AAACAGATGT ATGTTGTCCT TCGGGGTCAT TCACTTTACC TGTACAAAGA TAAAAGAGAG 
CAGACGACTC CGTCTGAGGA AGAGCAGCCC ATCAGTGTTA ATGCTTGCTT GATAGACATC 
TCTTACAGTG AGACCAAGAG GAAAAATGTG TTTCGACTCA CCACGTCCGA CTGTGAATGC 
CTGTTTCAGG CTGAAGACAG AGATGATATG CTAGCTTGGA TCAAGACGAT CCAGGAGAGC 
AGCAACCTAA ACGAAGAGGA CACTGGAGTC ACTAACAGGG ATCTAATTAG TCGAAGAATA 
AAAGAATACA ACAATCTGAT GAGCAAAGCA GAACAGTTGC CAAAAACACC TCGCCAGAGT 
CTCAGCATCA GGCAAACTTT GCTTGGTGCT AAATCAGAGC CAAAGACTCA AAGCCCACAC 
TCTCCGAAGG AAGAGTCGGA AAGGAAACTT CTCAGTAAAG ATGATACCAG TCCCCCAAAA 
GACAAAGGCA CATGGAGAAA AGGCATTCCA AGTATCATGA GAAAGACATT TGAGAAAAAG 
CCAACTGCTA CAGGAACTTT CGGCGTCCGA CTAGATGACT GCCCACCAGC TCATACTAAT 
CGGTATATTC CATTAATAGT TGACATATGT TGCAAATTAG TTGAAGAAAG AGGTCTTGAA 
TATACAGGTA TTTATAGAGT TCCTGGAAAT AATGCAGCCA TCTCAAGTAT GCAAGAAGAA 
CTCAACAAGG GAATGGCTGA TATTGATATA CAAGATGATA AATGGCGAGA TTTGAATGTG 
ATAAGCAGTT TACTAAAATC CTTCTTCAGA AAACTCCCTG AGCCTCTCTT CACAAATGAT 
AAATATGCTG ATTTTATTGA AGCCAATCGT AAAGAAGATC CTCTAGATCG TCTGAAAACA 
TTAAAAAGAC TAATTCACGA TTTGCCTGAA CATCATTATG AAACACTTAA GTTCCTTTCA 
GCTCATCTGA AGACAGTGGC AGAAAATTCA GAAAAAAATA AGATGGAACC AAGAAACCTA 
GCAATAGTGT TTGGTCCCAC CCTTGTTCGA ACATCAGAAG ACAACATGAC CCACATGGTC 
ACCCACATGC CTGACCAGTA CAAGATTGTA GAAACGCTCA TCCAGCACCA TGACTGGTTT 
TTCACAGAAG AAGGTGCTGA AGAGCCTCTT ACAACAGTGC AGGAGGAAAG CACAGTAGAC 
TCCCAGCCAG TGCCAAACAT AGATCATTTA CTCACCAACA TTGGAAGGAC AGGAGTCTCC 
CCAGGAGATG TATCAGATTC AGCTACTAGT GACTCAACAA AATCTAAGGG TTCTTGGGGA 
TCTGGAAAGG ATCAGTATAG CAGGGAACTG CTTGTGTCCT CCATCTTTGC AGCTGCTAGT 
CGCAAGAGGA AGAAGCCGAA AGAAAAAGCA CAGCCTAGCA GCTCAGAAGA TGAACTGGAC 
AATGTATTTT TTAAGAAAGA AAATGTGGAA CAGTGTCACA ATGATACTAA AGAGGAGTCC 
AAAAAAGAAA GTGAGACACT GGGCAGAAAA CAGAAGATCA TCATTGCCAA AGAAAACAGC 
ACTAGGAAAG ACCCCAGCAC GACAAAAGAT GAAAAGATAT CACTAGGAAA AGAGAGCACG 
CCTTCTGAAG AACCCTCACC ACCACACAAC TCAAAACACA ACAAGTCACC AACTCTCAGC 
TGTCGCTTTG CCATCCTGAA AGAGAGCCCC AGGTCACTTC TGGCACAGAA GTCCTCCCAC 
CTTGAAGAGA CAGGCTCTGA CTCTGGCACT TTGCTCAGCA CGTCTTCCCA GGCCTCCCTG 
GCAAGGTTTT CCATGAAGAA ATCAACCAGT CCAGAAACGA AACATAGCGA GTTTTTGGCC 
AACGTCAGCA CCATCACCTC AGATTATTCC ACCACATCGT CTGCTACATA CTTGACTAGC 
CTGGACTCCA GTCGACTGAG CCCTGAGGTG CAATCCGTGG CAGAGAGCAA GGGGGACGAG 
GCAGATGACG AGAGAAGCGA ACTCATCAGT GAAGGGCGGC CTGTGGAAAC CGACAGCGAG 
AGCGAGTTTC CCGTGTTCCC CACAGCCTTG ACTTCAGAGA GGCTTTTCCG AGGAAAACTG 
CAAGAAGTGA CTAAGAGCAG CCGGAGAAAT TCTGAAGGAA GTGAATTAAG TTGCACCGAG 
GGAAGTTTAA CATCAAGTTT AGATAGCCGG AGACAGCTCT TCAGTTCCCA TAAACTCATC 
GAATGTGATA CTCTTTCCAG GAAAAAATCA GCTAGATTCA AGTCAGATAG TGGAAGTCTA 
GGAGATGCCA AGAATGAGAA AGAAGCACCT TCGTTAACTA AAGTGTTTGA TGTTATGAAA 
AAAGGAAAGT CAACTGGGAG TTTACTGACA CCCACCAGAG GCGAATCCGA AAAACAGGAA 
CCCACATGGA AAACGAAAAT AGCAGATCGG TTAAAACTGA GACCCAGAGC CCCTGCGGAT 
GACATGTTTG GAGTAGGGAA TCACAAAGTG AATGCCGAGA CTGCTAAAAG GAAAAGCATC 
CGGCGCAGAC ATACACTAGG AGGGCACAGA GATGCTACCG AAATCAGCGT TTTGAATTTT 
TGGAAAGTGC ATGAGCAGAG CGGGGAGAGA GAATCTGAAC TTTCAGCTGT AAACCGGTTA 
AAACCAAAAT GCTCAGCCCA GGACCTTTCC ATCTCAGACT GGCTGGCCAG GGAACGCCTA 
CGCACCAGTA CCTCTGACCT TAGCAGAGGA GAAATCGGAG ATCCCCAGAC AGAGAACCCA 
AGCACACGAG AAATAGCCAC GACCGACACA CCTTTGTCTC TTCATTGCAA CACAGGCAGT 
TCTTCCAGCA CCTTGGCTTC AACAAACAGG CCCCTTCTTT CCATACCACC ACAGTCACCT 
GACCAAATAA ACGGAGAAAG CTTCCAGAAC GTGAGCAAAA ATGCTAGTTC TGCAGCGAAT 
GCCCAACCTC ATAAACTGTC TGAAACCCCA GGCAGTAAAG CAGAGTTTCA TCCCTGTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367452.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354381.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 9, mRNA.

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CloneID	OHu107722
Clone ID Related Accession (Same CDS sequence)	NM_001367452.1
Accession Version	NM_001367452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.135346.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAAA TGGAAACAGA GGAGCTACAG TTTACAAAGG ATGTCACAGC ACTGGCATAT 
TCTCAAGATG CCTACCTGAA AGGCAACGAA GCTTATAGCG GCAATGCCCG CAATATACCT 
GAACCTCCAC CAATCTGCTA TCCCTGGCTG CCATCTGCCC CATCAGCCAT GGCACAGCCA 
GTTGAAATAT CTCCTCCTGA CTCATCATTG AGCAAACAGC AAACCAGTAC ACCAGTACTG 
ACACAACCTG GTAGGGCCTA TAGAATGGAA ATACAAGTGC CTCCATCACC AACAGATGTT 
GCAAAATCAA ACACAGCAGT GTGTGTTTGC AATGAAAGTG TAAGGACTGT CATTGTGCCT 
TCTGAGAAGG TTGTAGATTT GTTATCCAAT AGAAACAACC ATACAGGTCC TTCACATAGA 
ACTGAAGAAG TGAGGTATGG CGTGAGTGAG CAGACCTCTT TAAAAACAGT GTCAAGAACC 
ACATCACCAC CATTATCAAT TCCCACCACT CATCTAATTC ATCAGCCTGC AGGCTCCAGA 
TCACTGGAAC CTTCTGGAAT TTTACTTAAG TCTGGAAATT ACAGTGGACA TTCTGATGGA 
ATCTCAAGCA GCAGATCTCA AGCTGTGGAG GCTCCCTCTG TATCTGTTAA TCACTATTCG 
CCAAATTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACAGACGAT TGCACATAGG TTGTCGGACA ATACAAGAAA GATTAGATAG TTTAAGAGCA 
GCATCTCAAA GCACGACAGA TTATAACCAG GTCGTCCCCA ACCGCACTAC TTTGCAGGGA 
CGACGTCGAA GCACCTCTCA TGATCGAGTG CCCCAGTCTG TCCAGATACG GCAACGCAGT 
GTGTCCCAAG AAAGACTGGA AGATTCTGTG CTAATGAAGT ATTGTCCAAG AAGTGCATCT 
CAAGGAGCAC TGACGTCTCC ATCTGTTAGT TTTAGTAATC ATAGAACTCG TTCATGGGAT 
TATATTGAGG GACAGGATGA AACCTTAGAA AATGTCAATT CTGGAACTCC AATACCTGAT 
TCCAATGGAG AGAAAAAACA GACTTACAAG TGGAGTGGGT TTACTGAACA GGATGATAGA 
CGAGGTATTT GTGAAAGACC TAGGCAGCAA GAAATTCATA AATCTTTTCG AGGTTCCAAT 
TTTACTGTGG CTCCAAGCGT TGTTAATTCT GATAACAGGC GAATGAGTGG TAGAGGAGTG 
GGATCTGTGT CGCAGTTTAA AAAAATTCCA CCAGATCTAA AAACATTGCA GTCAAACAGA 
AATTTTCAGA CTACTTGTGG AATGTCACTG CCTCGGGGTA TTTCACAAGA CAGGTCACCT 
CTTGTGAAAG TCCGAAGTAA TTCTCTGAAA GCTCCTTCCA CGCATGTCAC AAAACCATCA 
TTTAGCCAGA AATCATTTGT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAG 
AACAGTCTGT TGAATCAGCA GACATGGGTA AGGACTGACA GTGCCCCCGA TCAGCAAGTG 
GAGACTGGGA AATCCCCCTC TTTATCTGGA GCCTCTGCCA AGCCTGCCCC TCAGTCGAGT 
GAAAACGCTG GTACTTCAGA TTTAGAACTA CCTGTCAGTC AAAGGAATCA AGATTTAAGT 
TTACAAGAGG CTGAAACTGA GCAATCAGAT ACTTTAGATA ATAAAGAAGC TGTCATCCTA 
AGGGAAAAAC CTCCATCTGG ACGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTGGCAGTAA ATGACCAGGA GACCGGGTCA GACACTACCT GCTGGCTGCC CAATGATGCA 
CGTCGAGAGG TCCACATAAA AAGAATGGAG GAAAGAAAAG CCTCGAGTAC CAGTCCGCCT 
GGCGATTCTT TGGCTTCCAT CCCATTTATA GATGAACCAA CTAGCCCTAG CATTGATCAT 
GATATTGCAC ATATCCCTGC CTCTGCTGTT ATATCAGCCT CTACCTCTCA GGTCCCCTCC 
ATAGCAACAG TTCCTCCTTG CCTCACAACT TCAGCTCCAT TAATTCGCCG TCAGCTCTCA 
CATGACCACG AATCTGTTGG CCCTCCTAGC CTGGATGCTC AGCCCAACTC AAAGACAGAA 
AGATCAAAAT CATATGATGA GGGTCTGGAT GATTACAGAG AAGATGCAAA ATTGTCCTTT 
AAGCACGTAT CTAGTCTGAA GGGAATCAAG ATCGCAGACA GCCAAAAGTC ATCAGAAGAC 
TCTGGGTCCA GAAAAGATTC TTCCTCAGAG GTCTTCAGTG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCC GACCCCTTGT CACCGATAAG GGCAAGCGAG TTGGTGGAAG TATTCGGCCA 
TGGAAACAGA TGTATGTTGT CCTTCGGGGT CATTCACTTT ACCTGTACAA AGATAAAAGA 
GAGCAGACGA CTCCGTCTGA GGAAGAGCAG CCCATCAGTG TTAATGCTTG CTTGATAGAC 
ATCTCTTACA GTGAGACCAA GAGGAAAAAT GTGTTTCGAC TCACCACGTC CGACTGTGAA 
TGCCTGTTTC AGGCTGAAGA CAGAGATGAT ATGCTAGCTT GGATCAAGAC GATCCAGGAG 
AGCAGCAACC TAAACGAAGA GGACACTGGA GTCACTAACA GGGATCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAATCT GATGAGCAAA GCAGAACAGT TGCCAAAAAC ACCTCGCCAG 
AGTCTCAGCA TCAGGCAAAC TTTGCTTGGT GCTAAATCAG AGCCAAAGAC TCAAAGCCCA 
CACTCTCCGA AGGAAGAGTC GGAAAGGAAA CTTCTCAGTA AAGATGATAC CAGTCCCCCA 
AAAGACAAAG GCACATGGAG AAAAGGCATT CCAAGTATCA TGAGAAAGAC ATTTGAGAAA 
AAGCCAACTG CTACAGGAAC TTTCGGCGTC CGACTAGATG ACTGCCCACC AGCTCATACT 
AATCGGTATA TTCCATTAAT AGTTGACATA TGTTGCAAAT TAGTTGAAGA AAGAGGTCTT 
GAATATACAG GTATTTATAG AGTTCCTGGA AATAATGCAG CCATCTCAAG TATGCAAGAA 
GAACTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATAAGCA GTTTACTAAA ATCCTTCTTC AGAAAACTCC CTGAGCCTCT CTTCACAAAT 
GATAAATATG CTGATTTTAT TGAAGCCAAT CGTAAAGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA CGATTTGCCT GAACATCATT ATGAAACACT TAAGTTCCTT 
TCAGCTCATC TGAAGACAGT GGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCAATAG TGTTTGGTCC CACCCTTGTT CGAACATCAG AAGACAACAT GACCCACATG 
GTCACCCACA TGCCTGACCA GTACAAGATT GTAGAAACGC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAGCCT CTTACAACAG TGCAGGAGGA AAGCACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGAGTC 
TCCCCAGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCTGGAA AGGATCAGTA TAGCAGGGAA CTGCTTGTGT CCTCCATCTT TGCAGCTGCT 
AGTCGCAAGA GGAAGAAGCC GAAAGAAAAA GCACAGCCTA GCAGCTCAGA AGATGAACTG 
GACAATGTAT TTTTTAAGAA AGAAAATGTG GAACAGTGTC ACAATGATAC TAAAGAGGAG 
TCCAAAAAAG AAAGTGAGAC ACTGGGCAGA AAACAGAAGA TCATCATTGC CAAAGAAAAC 
AGCACTAGGA AAGACCCCAG CACGACAAAA GATGAAAAGA TATCACTAGG AAAAGAGAGC 
ACGCCTTCTG AAGAACCCTC ACCACCACAC AACTCAAAAC ACAACAAGTC ACCAACTCTC 
AGCTGTCGCT TTGCCATCCT GAAAGAGAGC CCCAGGTCAC TTCTGGCACA GAAGTCCTCC 
CACCTTGAAG AGACAGGCTC TGACTCTGGC ACTTTGCTCA GCACGTCTTC CCAGGCCTCC 
CTGGCAAGGT TTTCCATGAA GAAATCAACC AGTCCAGAAA CGAAACATAG CGAGTTTTTG 
GCCAACGTCA GCACCATCAC CTCAGATTAT TCCACCACAT CGTCTGCTAC ATACTTGACT 
AGCCTGGACT CCAGTCGACT GAGCCCTGAG GTGCAATCCG TGGCAGAGAG CAAGGGGGAC 
GAGGCAGATG ACGAGAGAAG CGAACTCATC AGTGAAGGGC GGCCTGTGGA AACCGACAGC 
GAGAGCGAGT TTCCCGTGTT CCCCACAGCC TTGACTTCAG AGAGGCTTTT CCGAGGAAAA 
CTGCAAGAAG TGACTAAGAG CAGCCGGAGA AATTCTGAAG GAAGTGAATT AAGTTGCACC 
GAGGGAAGTT TAACATCAAG TTTAGATAGC CGGAGACAGC TCTTCAGTTC CCATAAACTC 
ATCGAATGTG ATACTCTTTC CAGGAAAAAA TCAGCTAGAT TCAAGTCAGA TAGTGGAAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCA CCTTCGTTAA CTAAAGTGTT TGATGTTATG 
AAAAAAGGAA AGTCAACTGG GAGTTTACTG ACACCCACCA GAGGCGAATC CGAAAAACAG 
GAACCCACAT GGAAAACGAA AATAGCAGAT CGGTTAAAAC TGAGACCCAG AGCCCCTGCG 
GATGACATGT TTGGAGTAGG GAATCACAAA GTGAATGCCG AGACTGCTAA AAGGAAAAGC 
ATCCGGCGCA GACATACACT AGGAGGGCAC AGAGATGCTA CCGAAATCAG CGTTTTGAAT 
TTTTGGAAAG TGCATGAGCA GAGCGGGGAG AGAGAATCTG AACTTTCAGC TGTAAACCGG 
TTAAAACCAA AATGCTCAGC CCAGGACCTT TCCATCTCAG ACTGGCTGGC CAGGGAACGC 
CTACGCACCA GTACCTCTGA CCTTAGCAGA GGAGAAATCG GAGATCCCCA GACAGAGAAC 
CCAAGCACAC GAGAAATAGC CACGACCGAC ACACCTTTGT CTCTTCATTG CAACACAGGC 
AGTTCTTCCA GCACCTTGGC TTCAACAAAC AGGCCCCTTC TTTCCATACC ACCACAGTCA 
CCTGACCAAA TAAACGGAGA AAGCTTCCAG AACGTGAGCA AAAATGCTAG TTCTGCAGCG 
AATGCCCAAC CTCATAAACT GTCTGAAACC CCAGGCAGTA AAGCAGAGTT TCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354381.1
CDS	636..6041
Translation	MKKMETEELQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367452.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367452.1

ORF Insert Sequence:

ATGAAGAAAA TGGAAACAGA GGAGCTACAG TTTACAAAGG ATGTCACAGC ACTGGCATAT 
TCTCAAGATG CCTACCTGAA AGGCAACGAA GCTTATAGCG GCAATGCCCG CAATATACCT 
GAACCTCCAC CAATCTGCTA TCCCTGGCTG CCATCTGCCC CATCAGCCAT GGCACAGCCA 
GTTGAAATAT CTCCTCCTGA CTCATCATTG AGCAAACAGC AAACCAGTAC ACCAGTACTG 
ACACAACCTG GTAGGGCCTA TAGAATGGAA ATACAAGTGC CTCCATCACC AACAGATGTT 
GCAAAATCAA ACACAGCAGT GTGTGTTTGC AATGAAAGTG TAAGGACTGT CATTGTGCCT 
TCTGAGAAGG TTGTAGATTT GTTATCCAAT AGAAACAACC ATACAGGTCC TTCACATAGA 
ACTGAAGAAG TGAGGTATGG CGTGAGTGAG CAGACCTCTT TAAAAACAGT GTCAAGAACC 
ACATCACCAC CATTATCAAT TCCCACCACT CATCTAATTC ATCAGCCTGC AGGCTCCAGA 
TCACTGGAAC CTTCTGGAAT TTTACTTAAG TCTGGAAATT ACAGTGGACA TTCTGATGGA 
ATCTCAAGCA GCAGATCTCA AGCTGTGGAG GCTCCCTCTG TATCTGTTAA TCACTATTCG 
CCAAATTCCC ATCAGCACAT AGACTGGAAA AACTATAAAA CTTACAAAGA GTATATTGAT 
AACAGACGAT TGCACATAGG TTGTCGGACA ATACAAGAAA GATTAGATAG TTTAAGAGCA 
GCATCTCAAA GCACGACAGA TTATAACCAG GTCGTCCCCA ACCGCACTAC TTTGCAGGGA 
CGACGTCGAA GCACCTCTCA TGATCGAGTG CCCCAGTCTG TCCAGATACG GCAACGCAGT 
GTGTCCCAAG AAAGACTGGA AGATTCTGTG CTAATGAAGT ATTGTCCAAG AAGTGCATCT 
CAAGGAGCAC TGACGTCTCC ATCTGTTAGT TTTAGTAATC ATAGAACTCG TTCATGGGAT 
TATATTGAGG GACAGGATGA AACCTTAGAA AATGTCAATT CTGGAACTCC AATACCTGAT 
TCCAATGGAG AGAAAAAACA GACTTACAAG TGGAGTGGGT TTACTGAACA GGATGATAGA 
CGAGGTATTT GTGAAAGACC TAGGCAGCAA GAAATTCATA AATCTTTTCG AGGTTCCAAT 
TTTACTGTGG CTCCAAGCGT TGTTAATTCT GATAACAGGC GAATGAGTGG TAGAGGAGTG 
GGATCTGTGT CGCAGTTTAA AAAAATTCCA CCAGATCTAA AAACATTGCA GTCAAACAGA 
AATTTTCAGA CTACTTGTGG AATGTCACTG CCTCGGGGTA TTTCACAAGA CAGGTCACCT 
CTTGTGAAAG TCCGAAGTAA TTCTCTGAAA GCTCCTTCCA CGCATGTCAC AAAACCATCA 
TTTAGCCAGA AATCATTTGT TTCTATCAAA GACCAAAGAC CAGTAAATCA CTTGCATCAG 
AACAGTCTGT TGAATCAGCA GACATGGGTA AGGACTGACA GTGCCCCCGA TCAGCAAGTG 
GAGACTGGGA AATCCCCCTC TTTATCTGGA GCCTCTGCCA AGCCTGCCCC TCAGTCGAGT 
GAAAACGCTG GTACTTCAGA TTTAGAACTA CCTGTCAGTC AAAGGAATCA AGATTTAAGT 
TTACAAGAGG CTGAAACTGA GCAATCAGAT ACTTTAGATA ATAAAGAAGC TGTCATCCTA 
AGGGAAAAAC CTCCATCTGG ACGCCAGACA CCGCAGCCTT TAAGGCATCA GTCTTACATC 
TTGGCAGTAA ATGACCAGGA GACCGGGTCA GACACTACCT GCTGGCTGCC CAATGATGCA 
CGTCGAGAGG TCCACATAAA AAGAATGGAG GAAAGAAAAG CCTCGAGTAC CAGTCCGCCT 
GGCGATTCTT TGGCTTCCAT CCCATTTATA GATGAACCAA CTAGCCCTAG CATTGATCAT 
GATATTGCAC ATATCCCTGC CTCTGCTGTT ATATCAGCCT CTACCTCTCA GGTCCCCTCC 
ATAGCAACAG TTCCTCCTTG CCTCACAACT TCAGCTCCAT TAATTCGCCG TCAGCTCTCA 
CATGACCACG AATCTGTTGG CCCTCCTAGC CTGGATGCTC AGCCCAACTC AAAGACAGAA 
AGATCAAAAT CATATGATGA GGGTCTGGAT GATTACAGAG AAGATGCAAA ATTGTCCTTT 
AAGCACGTAT CTAGTCTGAA GGGAATCAAG ATCGCAGACA GCCAAAAGTC ATCAGAAGAC 
TCTGGGTCCA GAAAAGATTC TTCCTCAGAG GTCTTCAGTG ATGCTGCCAA GGAAGGGTGG 
CTTCATTTCC GACCCCTTGT CACCGATAAG GGCAAGCGAG TTGGTGGAAG TATTCGGCCA 
TGGAAACAGA TGTATGTTGT CCTTCGGGGT CATTCACTTT ACCTGTACAA AGATAAAAGA 
GAGCAGACGA CTCCGTCTGA GGAAGAGCAG CCCATCAGTG TTAATGCTTG CTTGATAGAC 
ATCTCTTACA GTGAGACCAA GAGGAAAAAT GTGTTTCGAC TCACCACGTC CGACTGTGAA 
TGCCTGTTTC AGGCTGAAGA CAGAGATGAT ATGCTAGCTT GGATCAAGAC GATCCAGGAG 
AGCAGCAACC TAAACGAAGA GGACACTGGA GTCACTAACA GGGATCTAAT TAGTCGAAGA 
ATAAAAGAAT ACAACAATCT GATGAGCAAA GCAGAACAGT TGCCAAAAAC ACCTCGCCAG 
AGTCTCAGCA TCAGGCAAAC TTTGCTTGGT GCTAAATCAG AGCCAAAGAC TCAAAGCCCA 
CACTCTCCGA AGGAAGAGTC GGAAAGGAAA CTTCTCAGTA AAGATGATAC CAGTCCCCCA 
AAAGACAAAG GCACATGGAG AAAAGGCATT CCAAGTATCA TGAGAAAGAC ATTTGAGAAA 
AAGCCAACTG CTACAGGAAC TTTCGGCGTC CGACTAGATG ACTGCCCACC AGCTCATACT 
AATCGGTATA TTCCATTAAT AGTTGACATA TGTTGCAAAT TAGTTGAAGA AAGAGGTCTT 
GAATATACAG GTATTTATAG AGTTCCTGGA AATAATGCAG CCATCTCAAG TATGCAAGAA 
GAACTCAACA AGGGAATGGC TGATATTGAT ATACAAGATG ATAAATGGCG AGATTTGAAT 
GTGATAAGCA GTTTACTAAA ATCCTTCTTC AGAAAACTCC CTGAGCCTCT CTTCACAAAT 
GATAAATATG CTGATTTTAT TGAAGCCAAT CGTAAAGAAG ATCCTCTAGA TCGTCTGAAA 
ACATTAAAAA GACTAATTCA CGATTTGCCT GAACATCATT ATGAAACACT TAAGTTCCTT 
TCAGCTCATC TGAAGACAGT GGCAGAAAAT TCAGAAAAAA ATAAGATGGA ACCAAGAAAC 
CTAGCAATAG TGTTTGGTCC CACCCTTGTT CGAACATCAG AAGACAACAT GACCCACATG 
GTCACCCACA TGCCTGACCA GTACAAGATT GTAGAAACGC TCATCCAGCA CCATGACTGG 
TTTTTCACAG AAGAAGGTGC TGAAGAGCCT CTTACAACAG TGCAGGAGGA AAGCACAGTA 
GACTCCCAGC CAGTGCCAAA CATAGATCAT TTACTCACCA ACATTGGAAG GACAGGAGTC 
TCCCCAGGAG ATGTATCAGA TTCAGCTACT AGTGACTCAA CAAAATCTAA GGGTTCTTGG 
GGATCTGGAA AGGATCAGTA TAGCAGGGAA CTGCTTGTGT CCTCCATCTT TGCAGCTGCT 
AGTCGCAAGA GGAAGAAGCC GAAAGAAAAA GCACAGCCTA GCAGCTCAGA AGATGAACTG 
GACAATGTAT TTTTTAAGAA AGAAAATGTG GAACAGTGTC ACAATGATAC TAAAGAGGAG 
TCCAAAAAAG AAAGTGAGAC ACTGGGCAGA AAACAGAAGA TCATCATTGC CAAAGAAAAC 
AGCACTAGGA AAGACCCCAG CACGACAAAA GATGAAAAGA TATCACTAGG AAAAGAGAGC 
ACGCCTTCTG AAGAACCCTC ACCACCACAC AACTCAAAAC ACAACAAGTC ACCAACTCTC 
AGCTGTCGCT TTGCCATCCT GAAAGAGAGC CCCAGGTCAC TTCTGGCACA GAAGTCCTCC 
CACCTTGAAG AGACAGGCTC TGACTCTGGC ACTTTGCTCA GCACGTCTTC CCAGGCCTCC 
CTGGCAAGGT TTTCCATGAA GAAATCAACC AGTCCAGAAA CGAAACATAG CGAGTTTTTG 
GCCAACGTCA GCACCATCAC CTCAGATTAT TCCACCACAT CGTCTGCTAC ATACTTGACT 
AGCCTGGACT CCAGTCGACT GAGCCCTGAG GTGCAATCCG TGGCAGAGAG CAAGGGGGAC 
GAGGCAGATG ACGAGAGAAG CGAACTCATC AGTGAAGGGC GGCCTGTGGA AACCGACAGC 
GAGAGCGAGT TTCCCGTGTT CCCCACAGCC TTGACTTCAG AGAGGCTTTT CCGAGGAAAA 
CTGCAAGAAG TGACTAAGAG CAGCCGGAGA AATTCTGAAG GAAGTGAATT AAGTTGCACC 
GAGGGAAGTT TAACATCAAG TTTAGATAGC CGGAGACAGC TCTTCAGTTC CCATAAACTC 
ATCGAATGTG ATACTCTTTC CAGGAAAAAA TCAGCTAGAT TCAAGTCAGA TAGTGGAAGT 
CTAGGAGATG CCAAGAATGA GAAAGAAGCA CCTTCGTTAA CTAAAGTGTT TGATGTTATG 
AAAAAAGGAA AGTCAACTGG GAGTTTACTG ACACCCACCA GAGGCGAATC CGAAAAACAG 
GAACCCACAT GGAAAACGAA AATAGCAGAT CGGTTAAAAC TGAGACCCAG AGCCCCTGCG 
GATGACATGT TTGGAGTAGG GAATCACAAA GTGAATGCCG AGACTGCTAA AAGGAAAAGC 
ATCCGGCGCA GACATACACT AGGAGGGCAC AGAGATGCTA CCGAAATCAG CGTTTTGAAT 
TTTTGGAAAG TGCATGAGCA GAGCGGGGAG AGAGAATCTG AACTTTCAGC TGTAAACCGG 
TTAAAACCAA AATGCTCAGC CCAGGACCTT TCCATCTCAG ACTGGCTGGC CAGGGAACGC 
CTACGCACCA GTACCTCTGA CCTTAGCAGA GGAGAAATCG GAGATCCCCA GACAGAGAAC 
CCAAGCACAC GAGAAATAGC CACGACCGAC ACACCTTTGT CTCTTCATTG CAACACAGGC 
AGTTCTTCCA GCACCTTGGC TTCAACAAAC AGGCCCCTTC TTTCCATACC ACCACAGTCA 
CCTGACCAAA TAAACGGAGA AAGCTTCCAG AACGTGAGCA AAAATGCTAG TTCTGCAGCG 
AATGCCCAAC CTCATAAACT GTCTGAAACC CCAGGCAGTA AAGCAGAGTT TCATCCCTGT 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367455.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354384.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 10, mRNA.

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CloneID	OHu107755
Clone ID Related Accession (Same CDS sequence)	NM_001367455.1
Accession Version	NM_001367455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL392104.19. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.226130.1, SRR1803614.74181.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACAGC CAGTTGAAAT ATCTCCTCCT GACTCATCAT TGAGCAAACA GCAAACCAGT 
ACACCAGTAC TGACACAACC TGGTAGGGCC TATAGAATGG AAATACAAGT GCCTCCATCA 
CCAACAGATG TTGCAAAATC AAACACAGCA GTGTGTGTTT GCAATGAAAG TGTAAGGACT 
GTCATTGTGC CTTCTGAGAA GGTTGTAGAT TTGTTATCCA ATAGAAACAA CCATACAGGT 
CCTTCACATA GAACTGAAGA AGTGAGGTAT GGCGTGAGTG AGCAGACCTC TTTAAAAACA 
GTGTCAAGAA CCACATCACC ACCATTATCA ATTCCCACCA CTCATCTAAT TCATCAGCCT 
GCAGGCTCCA GATCACTGGA ACCTTCTGGA ATTTTACTTA AGTCTGGAAA TTACAGTGGA 
CATTCTGATG GAATCTCAAG CAGCAGATCT CAAGCTGTGG AGGCTCCCTC TGTATCTGTT 
AATCACTATT CGCCAAATTC CCATCAGCAC ATAGACTGGA AAAACTATAA AACTTACAAA 
GAGTATATTG ATAACAGACG ATTGCACATA GGTTGTCGGA CAATACAAGA AAGATTAGAT 
AGTTTAAGAG CAGCATCTCA AAGCACGACA GATTATAACC AGGTCGTCCC CAACCGCACT 
ACTTTGCAGG GACGACGTCG AAGCACCTCT CATGATCGAG TGCCCCAGTC TGTCCAGATA 
CGGCAACGCA GTGTGTCCCA AGAAAGACTG GAAGATTCTG TGCTAATGAA GTATTGTCCA 
AGAAGTGCAT CTCAAGGAGC ACTGACGTCT CCATCTGTTA GTTTTAGTAA TCATAGAACT 
CGTTCATGGG ATTATATTGA GGGACAGGAT GAAACCTTAG AAAATGTCAA TTCTGGAACT 
CCAATACCTG ATTCCAATGG AGAGAAAAAA CAGACTTACA AGTGGAGTGG GTTTACTGAA 
CAGGATGATA GACGAGGTAT TTGTGAAAGA CCTAGGCAGC AAGAAATTCA TAAATCTTTT 
CGAGGTTCCA ATTTTACTGT GGCTCCAAGC GTTGTTAATT CTGATAACAG GCGAATGAGT 
GGTAGAGGAG TGGGATCTGT GTCGCAGTTT AAAAAAATTC CACCAGATCT AAAAACATTG 
CAGTCAAACA GAAATTTTCA GACTACTTGT GGAATGTCAC TGCCTCGGGG TATTTCACAA 
GACAGGTCAC CTCTTGTGAA AGTCCGAAGT AATTCTCTGA AAGCTCCTTC CACGCATGTC 
ACAAAACCAT CATTTAGCCA GAAATCATTT GTTTCTATCA AAGACCAAAG ACCAGTAAAT 
CACTTGCATC AGAACAGTCT GTTGAATCAG CAGACATGGG TAAGGACTGA CAGTGCCCCC 
GATCAGCAAG TGGAGACTGG GAAATCCCCC TCTTTATCTG GAGCCTCTGC CAAGCCTGCC 
CCTCAGTCGA GTGAAAACGC TGGTACTTCA GATTTAGAAC TACCTGTCAG TCAAAGGAAT 
CAAGATTTAA GTTTACAAGA GGCTGAAACT GAGCAATCAG ATACTTTAGA TAATAAAGAA 
GCTGTCATCC TAAGGGAAAA ACCTCCATCT GGACGCCAGA CACCGCAGCC TTTAAGGCAT 
CAGTCTTACA TCTTGGCAGT AAATGACCAG GAGACCGGGT CAGACACTAC CTGCTGGCTG 
CCCAATGATG CACGTCGAGA GGTCCACATA AAAAGAATGG AGGAAAGAAA AGCCTCGAGT 
ACCAGTCCGC CTGGCGATTC TTTGGCTTCC ATCCCATTTA TAGATGAACC AACTAGCCCT 
AGCATTGATC ATGATATTGC ACATATCCCT GCCTCTGCTG TTATATCAGC CTCTACCTCT 
CAGGTCCCCT CCATAGCAAC AGTTCCTCCT TGCCTCACAA CTTCAGCTCC ATTAATTCGC 
CGTCAGCTCT CACATGACCA CGAATCTGTT GGCCCTCCTA GCCTGGATGC TCAGCCCAAC 
TCAAAGACAG AAAGATCAAA ATCATATGAT GAGGGTCTGG ATGATTACAG AGAAGATGCA 
AAATTGTCCT TTAAGCACGT ATCTAGTCTG AAGGGAATCA AGATCGCAGA CAGCCAAAAG 
TCATCAGAAG ACTCTGGGTC CAGAAAAGAT TCTTCCTCAG AGGTCTTCAG TGATGCTGCC 
AAGGAAGGGT GGCTTCATTT CCGACCCCTT GTCACCGATA AGGGCAAGCG AGTTGGTGGA 
AGTATTCGGC CATGGAAACA GATGTATGTT GTCCTTCGGG GTCATTCACT TTACCTGTAC 
AAAGATAAAA GAGAGCAGAC GACTCCGTCT GAGGAAGAGC AGCCCATCAG TGTTAATGCT 
TGCTTGATAG ACATCTCTTA CAGTGAGACC AAGAGGAAAA ATGTGTTTCG ACTCACCACG 
TCCGACTGTG AATGCCTGTT TCAGGCTGAA GACAGAGATG ATATGCTAGC TTGGATCAAG 
ACGATCCAGG AGAGCAGCAA CCTAAACGAA GAGGACACTG GAGTCACTAA CAGGGATCTA 
ATTAGTCGAA GAATAAAAGA ATACAACAAT CTGATGAGCA AAGCAGAACA GTTGCCAAAA 
ACACCTCGCC AGAGTCTCAG CATCAGGCAA ACTTTGCTTG GTGCTAAATC AGAGCCAAAG 
ACTCAAAGCC CACACTCTCC GAAGGAAGAG TCGGAAAGGA AACTTCTCAG TAAAGATGAT 
ACCAGTCCCC CAAAAGACAA AGGCACATGG AGAAAAGGCA TTCCAAGTAT CATGAGAAAG 
ACATTTGAGA AAAAGCCAAC TGCTACAGGA ACTTTCGGCG TCCGACTAGA TGACTGCCCA 
CCAGCTCATA CTAATCGGTA TATTCCATTA ATAGTTGACA TATGTTGCAA ATTAGTTGAA 
GAAAGAGGTC TTGAATATAC AGGTATTTAT AGAGTTCCTG GAAATAATGC AGCCATCTCA 
AGTATGCAAG AAGAACTCAA CAAGGGAATG GCTGATATTG ATATACAAGA TGATAAATGG 
CGAGATTTGA ATGTGATAAG CAGTTTACTA AAATCCTTCT TCAGAAAACT CCCTGAGCCT 
CTCTTCACAA ATGATAAATA TGCTGATTTT ATTGAAGCCA ATCGTAAAGA AGATCCTCTA 
GATCGTCTGA AAACATTAAA AAGACTAATT CACGATTTGC CTGAACATCA TTATGAAACA 
CTTAAGTTCC TTTCAGCTCA TCTGAAGACA GTGGCAGAAA ATTCAGAAAA AAATAAGATG 
GAACCAAGAA ACCTAGCAAT AGTGTTTGGT CCCACCCTTG TTCGAACATC AGAAGACAAC 
ATGACCCACA TGGTCACCCA CATGCCTGAC CAGTACAAGA TTGTAGAAAC GCTCATCCAG 
CACCATGACT GGTTTTTCAC AGAAGAAGGT GCTGAAGAGC CTCTTACAAC AGTGCAGGAG 
GAAAGCACAG TAGACTCCCA GCCAGTGCCA AACATAGATC ATTTACTCAC CAACATTGGA 
AGGACAGGAG TCTCCCCAGG AGATGTATCA GATTCAGCTA CTAGTGACTC AACAAAATCT 
AAGGGTTCTT GGGGATCTGG AAAGGATCAG TATAGCAGGG AACTGCTTGT GTCCTCCATC 
TTTGCAGCTG CTAGTCGCAA GAGGAAGAAG CCGAAAGAAA AAGCACAGCC TAGCAGCTCA 
GAAGATGAAC TGGACAATGT ATTTTTTAAG AAAGAAAATG TGGAACAGTG TCACAATGAT 
ACTAAAGAGG AGTCCAAAAA AGAAAGTGAG ACACTGGGCA GAAAACAGAA GATCATCATT 
GCCAAAGAAA ACAGCACTAG GAAAGACCCC AGCACGACAA AAGATGAAAA GATATCACTA 
GGAAAAGAGA GCACGCCTTC TGAAGAACCC TCACCACCAC ACAACTCAAA ACACAACAAG 
TCACCAACTC TCAGCTGTCG CTTTGCCATC CTGAAAGAGA GCCCCAGGTC ACTTCTGGCA 
CAGAAGTCCT CCCACCTTGA AGAGACAGGC TCTGACTCTG GCACTTTGCT CAGCACGTCT 
TCCCAGGCCT CCCTGGCAAG GTTTTCCATG AAGAAATCAA CCAGTCCAGA AACGAAACAT 
AGCGAGTTTT TGGCCAACGT CAGCACCATC ACCTCAGATT ATTCCACCAC ATCGTCTGCT 
ACATACTTGA CTAGCCTGGA CTCCAGTCGA CTGAGCCCTG AGGTGCAATC CGTGGCAGAG 
AGCAAGGGGG ACGAGGCAGA TGACGAGAGA AGCGAACTCA TCAGTGAAGG GCGGCCTGTG 
GAAACCGACA GCGAGAGCGA GTTTCCCGTG TTCCCCACAG CCTTGACTTC AGAGAGGCTT 
TTCCGAGGAA AACTGCAAGA AGTGACTAAG AGCAGCCGGA GAAATTCTGA AGGAAGTGAA 
TTAAGTTGCA CCGAGGGAAG TTTAACATCA AGTTTAGATA GCCGGAGACA GCTCTTCAGT 
TCCCATAAAC TCATCGAATG TGATACTCTT TCCAGGAAAA AATCAGCTAG ATTCAAGTCA 
GATAGTGGAA GTCTAGGAGA TGCCAAGAAT GAGAAAGAAG CACCTTCGTT AACTAAAGTG 
TTTGATGTTA TGAAAAAAGG AAAGTCAACT GGGAGTTTAC TGACACCCAC CAGAGGCGAA 
TCCGAAAAAC AGGAACCCAC ATGGAAAACG AAAATAGCAG ATCGGTTAAA ACTGAGACCC 
AGAGCCCCTG CGGATGACAT GTTTGGAGTA GGGAATCACA AAGTGAATGC CGAGACTGCT 
AAAAGGAAAA GCATCCGGCG CAGACATACA CTAGGAGGGC ACAGAGATGC TACCGAAATC 
AGCGTTTTGA ATTTTTGGAA AGTGCATGAG CAGAGCGGGG AGAGAGAATC TGAACTTTCA 
GCTGTAAACC GGTTAAAACC AAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTG 
GCCAGGGAAC GCCTACGCAC CAGTACCTCT GACCTTAGCA GAGGAGAAAT CGGAGATCCC 
CAGACAGAGA ACCCAAGCAC ACGAGAAATA GCCACGACCG ACACACCTTT GTCTCTTCAT 
TGCAACACAG GCAGTTCTTC CAGCACCTTG GCTTCAACAA ACAGGCCCCT TCTTTCCATA 
CCACCACAGT CACCTGACCA AATAAACGGA GAAAGCTTCC AGAACGTGAG CAAAAATGCT 
AGTTCTGCAG CGAATGCCCA ACCTCATAAA CTGTCTGAAA CCCCAGGCAG TAAAGCAGAG 
TTTCATCCCT GTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354384.1
CDS	226..5463
Translation	MAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367455.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367455.1

ORF Insert Sequence:

ATGGCACAGC CAGTTGAAAT ATCTCCTCCT GACTCATCAT TGAGCAAACA GCAAACCAGT 
ACACCAGTAC TGACACAACC TGGTAGGGCC TATAGAATGG AAATACAAGT GCCTCCATCA 
CCAACAGATG TTGCAAAATC AAACACAGCA GTGTGTGTTT GCAATGAAAG TGTAAGGACT 
GTCATTGTGC CTTCTGAGAA GGTTGTAGAT TTGTTATCCA ATAGAAACAA CCATACAGGT 
CCTTCACATA GAACTGAAGA AGTGAGGTAT GGCGTGAGTG AGCAGACCTC TTTAAAAACA 
GTGTCAAGAA CCACATCACC ACCATTATCA ATTCCCACCA CTCATCTAAT TCATCAGCCT 
GCAGGCTCCA GATCACTGGA ACCTTCTGGA ATTTTACTTA AGTCTGGAAA TTACAGTGGA 
CATTCTGATG GAATCTCAAG CAGCAGATCT CAAGCTGTGG AGGCTCCCTC TGTATCTGTT 
AATCACTATT CGCCAAATTC CCATCAGCAC ATAGACTGGA AAAACTATAA AACTTACAAA 
GAGTATATTG ATAACAGACG ATTGCACATA GGTTGTCGGA CAATACAAGA AAGATTAGAT 
AGTTTAAGAG CAGCATCTCA AAGCACGACA GATTATAACC AGGTCGTCCC CAACCGCACT 
ACTTTGCAGG GACGACGTCG AAGCACCTCT CATGATCGAG TGCCCCAGTC TGTCCAGATA 
CGGCAACGCA GTGTGTCCCA AGAAAGACTG GAAGATTCTG TGCTAATGAA GTATTGTCCA 
AGAAGTGCAT CTCAAGGAGC ACTGACGTCT CCATCTGTTA GTTTTAGTAA TCATAGAACT 
CGTTCATGGG ATTATATTGA GGGACAGGAT GAAACCTTAG AAAATGTCAA TTCTGGAACT 
CCAATACCTG ATTCCAATGG AGAGAAAAAA CAGACTTACA AGTGGAGTGG GTTTACTGAA 
CAGGATGATA GACGAGGTAT TTGTGAAAGA CCTAGGCAGC AAGAAATTCA TAAATCTTTT 
CGAGGTTCCA ATTTTACTGT GGCTCCAAGC GTTGTTAATT CTGATAACAG GCGAATGAGT 
GGTAGAGGAG TGGGATCTGT GTCGCAGTTT AAAAAAATTC CACCAGATCT AAAAACATTG 
CAGTCAAACA GAAATTTTCA GACTACTTGT GGAATGTCAC TGCCTCGGGG TATTTCACAA 
GACAGGTCAC CTCTTGTGAA AGTCCGAAGT AATTCTCTGA AAGCTCCTTC CACGCATGTC 
ACAAAACCAT CATTTAGCCA GAAATCATTT GTTTCTATCA AAGACCAAAG ACCAGTAAAT 
CACTTGCATC AGAACAGTCT GTTGAATCAG CAGACATGGG TAAGGACTGA CAGTGCCCCC 
GATCAGCAAG TGGAGACTGG GAAATCCCCC TCTTTATCTG GAGCCTCTGC CAAGCCTGCC 
CCTCAGTCGA GTGAAAACGC TGGTACTTCA GATTTAGAAC TACCTGTCAG TCAAAGGAAT 
CAAGATTTAA GTTTACAAGA GGCTGAAACT GAGCAATCAG ATACTTTAGA TAATAAAGAA 
GCTGTCATCC TAAGGGAAAA ACCTCCATCT GGACGCCAGA CACCGCAGCC TTTAAGGCAT 
CAGTCTTACA TCTTGGCAGT AAATGACCAG GAGACCGGGT CAGACACTAC CTGCTGGCTG 
CCCAATGATG CACGTCGAGA GGTCCACATA AAAAGAATGG AGGAAAGAAA AGCCTCGAGT 
ACCAGTCCGC CTGGCGATTC TTTGGCTTCC ATCCCATTTA TAGATGAACC AACTAGCCCT 
AGCATTGATC ATGATATTGC ACATATCCCT GCCTCTGCTG TTATATCAGC CTCTACCTCT 
CAGGTCCCCT CCATAGCAAC AGTTCCTCCT TGCCTCACAA CTTCAGCTCC ATTAATTCGC 
CGTCAGCTCT CACATGACCA CGAATCTGTT GGCCCTCCTA GCCTGGATGC TCAGCCCAAC 
TCAAAGACAG AAAGATCAAA ATCATATGAT GAGGGTCTGG ATGATTACAG AGAAGATGCA 
AAATTGTCCT TTAAGCACGT ATCTAGTCTG AAGGGAATCA AGATCGCAGA CAGCCAAAAG 
TCATCAGAAG ACTCTGGGTC CAGAAAAGAT TCTTCCTCAG AGGTCTTCAG TGATGCTGCC 
AAGGAAGGGT GGCTTCATTT CCGACCCCTT GTCACCGATA AGGGCAAGCG AGTTGGTGGA 
AGTATTCGGC CATGGAAACA GATGTATGTT GTCCTTCGGG GTCATTCACT TTACCTGTAC 
AAAGATAAAA GAGAGCAGAC GACTCCGTCT GAGGAAGAGC AGCCCATCAG TGTTAATGCT 
TGCTTGATAG ACATCTCTTA CAGTGAGACC AAGAGGAAAA ATGTGTTTCG ACTCACCACG 
TCCGACTGTG AATGCCTGTT TCAGGCTGAA GACAGAGATG ATATGCTAGC TTGGATCAAG 
ACGATCCAGG AGAGCAGCAA CCTAAACGAA GAGGACACTG GAGTCACTAA CAGGGATCTA 
ATTAGTCGAA GAATAAAAGA ATACAACAAT CTGATGAGCA AAGCAGAACA GTTGCCAAAA 
ACACCTCGCC AGAGTCTCAG CATCAGGCAA ACTTTGCTTG GTGCTAAATC AGAGCCAAAG 
ACTCAAAGCC CACACTCTCC GAAGGAAGAG TCGGAAAGGA AACTTCTCAG TAAAGATGAT 
ACCAGTCCCC CAAAAGACAA AGGCACATGG AGAAAAGGCA TTCCAAGTAT CATGAGAAAG 
ACATTTGAGA AAAAGCCAAC TGCTACAGGA ACTTTCGGCG TCCGACTAGA TGACTGCCCA 
CCAGCTCATA CTAATCGGTA TATTCCATTA ATAGTTGACA TATGTTGCAA ATTAGTTGAA 
GAAAGAGGTC TTGAATATAC AGGTATTTAT AGAGTTCCTG GAAATAATGC AGCCATCTCA 
AGTATGCAAG AAGAACTCAA CAAGGGAATG GCTGATATTG ATATACAAGA TGATAAATGG 
CGAGATTTGA ATGTGATAAG CAGTTTACTA AAATCCTTCT TCAGAAAACT CCCTGAGCCT 
CTCTTCACAA ATGATAAATA TGCTGATTTT ATTGAAGCCA ATCGTAAAGA AGATCCTCTA 
GATCGTCTGA AAACATTAAA AAGACTAATT CACGATTTGC CTGAACATCA TTATGAAACA 
CTTAAGTTCC TTTCAGCTCA TCTGAAGACA GTGGCAGAAA ATTCAGAAAA AAATAAGATG 
GAACCAAGAA ACCTAGCAAT AGTGTTTGGT CCCACCCTTG TTCGAACATC AGAAGACAAC 
ATGACCCACA TGGTCACCCA CATGCCTGAC CAGTACAAGA TTGTAGAAAC GCTCATCCAG 
CACCATGACT GGTTTTTCAC AGAAGAAGGT GCTGAAGAGC CTCTTACAAC AGTGCAGGAG 
GAAAGCACAG TAGACTCCCA GCCAGTGCCA AACATAGATC ATTTACTCAC CAACATTGGA 
AGGACAGGAG TCTCCCCAGG AGATGTATCA GATTCAGCTA CTAGTGACTC AACAAAATCT 
AAGGGTTCTT GGGGATCTGG AAAGGATCAG TATAGCAGGG AACTGCTTGT GTCCTCCATC 
TTTGCAGCTG CTAGTCGCAA GAGGAAGAAG CCGAAAGAAA AAGCACAGCC TAGCAGCTCA 
GAAGATGAAC TGGACAATGT ATTTTTTAAG AAAGAAAATG TGGAACAGTG TCACAATGAT 
ACTAAAGAGG AGTCCAAAAA AGAAAGTGAG ACACTGGGCA GAAAACAGAA GATCATCATT 
GCCAAAGAAA ACAGCACTAG GAAAGACCCC AGCACGACAA AAGATGAAAA GATATCACTA 
GGAAAAGAGA GCACGCCTTC TGAAGAACCC TCACCACCAC ACAACTCAAA ACACAACAAG 
TCACCAACTC TCAGCTGTCG CTTTGCCATC CTGAAAGAGA GCCCCAGGTC ACTTCTGGCA 
CAGAAGTCCT CCCACCTTGA AGAGACAGGC TCTGACTCTG GCACTTTGCT CAGCACGTCT 
TCCCAGGCCT CCCTGGCAAG GTTTTCCATG AAGAAATCAA CCAGTCCAGA AACGAAACAT 
AGCGAGTTTT TGGCCAACGT CAGCACCATC ACCTCAGATT ATTCCACCAC ATCGTCTGCT 
ACATACTTGA CTAGCCTGGA CTCCAGTCGA CTGAGCCCTG AGGTGCAATC CGTGGCAGAG 
AGCAAGGGGG ACGAGGCAGA TGACGAGAGA AGCGAACTCA TCAGTGAAGG GCGGCCTGTG 
GAAACCGACA GCGAGAGCGA GTTTCCCGTG TTCCCCACAG CCTTGACTTC AGAGAGGCTT 
TTCCGAGGAA AACTGCAAGA AGTGACTAAG AGCAGCCGGA GAAATTCTGA AGGAAGTGAA 
TTAAGTTGCA CCGAGGGAAG TTTAACATCA AGTTTAGATA GCCGGAGACA GCTCTTCAGT 
TCCCATAAAC TCATCGAATG TGATACTCTT TCCAGGAAAA AATCAGCTAG ATTCAAGTCA 
GATAGTGGAA GTCTAGGAGA TGCCAAGAAT GAGAAAGAAG CACCTTCGTT AACTAAAGTG 
TTTGATGTTA TGAAAAAAGG AAAGTCAACT GGGAGTTTAC TGACACCCAC CAGAGGCGAA 
TCCGAAAAAC AGGAACCCAC ATGGAAAACG AAAATAGCAG ATCGGTTAAA ACTGAGACCC 
AGAGCCCCTG CGGATGACAT GTTTGGAGTA GGGAATCACA AAGTGAATGC CGAGACTGCT 
AAAAGGAAAA GCATCCGGCG CAGACATACA CTAGGAGGGC ACAGAGATGC TACCGAAATC 
AGCGTTTTGA ATTTTTGGAA AGTGCATGAG CAGAGCGGGG AGAGAGAATC TGAACTTTCA 
GCTGTAAACC GGTTAAAACC AAAATGCTCA GCCCAGGACC TTTCCATCTC AGACTGGCTG 
GCCAGGGAAC GCCTACGCAC CAGTACCTCT GACCTTAGCA GAGGAGAAAT CGGAGATCCC 
CAGACAGAGA ACCCAAGCAC ACGAGAAATA GCCACGACCG ACACACCTTT GTCTCTTCAT 
TGCAACACAG GCAGTTCTTC CAGCACCTTG GCTTCAACAA ACAGGCCCCT TCTTTCCATA 
CCACCACAGT CACCTGACCA AATAAACGGA GAAAGCTTCC AGAACGTGAG CAAAAATGCT 
AGTTCTGCAG CGAATGCCCA ACCTCATAAA CTGTCTGAAA CCCCAGGCAG TAAAGCAGAG 
TTTCATCCCT GTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_024448088.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303856.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X11, mRNA.

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CloneID	OHu106538
Clone ID Related Accession (Same CDS sequence)	XM_024448088.1
Accession Version	XM_024448088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTC TGTGCAGCTG GTCAGTCTCT CAGCCATTAC TTTGGAAACA GGAAACGCCT 
CAAGGAAAAC AAAGAAACCG CTTGGAACCA ATGGATACCA TATTTGTTAA GCAAGTTAAA 
GAAGGAGGAC CTGCTTTTGA AGCTGGATTA TGTACAGGTG ACCGAATTAT AAAAGTCAAT 
GGAGAAAGTG TTATTGGCAA AACCTATTCC CAAGTAATTG CTTTAATTCA AAACAGTGAT 
ACAACATTGG AACTTAGTGT TATGCCAAAA GATGAAGACA TTCTCCAAGT GGCATATTCT 
CAAGATGCCT ACCTGAAAGG CAACGAAGCT TATAGCGGCA ATGCCCGCAA TATACCTGAA 
CCTCCACCAA TCTGCTATCC CTGGCTGCCA TCTGCCCCAT CAGCCATGGC ACAGCCAGTT 
GAAATATCTC CTCCTGACTC ATCATTGAGC AAACAGCAAA CCAGTACACC AGTACTGACA 
CAACCTGGTA GGGCCTATAG AATGGAAATA CAAGTGCCTC CATCACCAAC AGATGTTGCA 
AAATCAAACA CAGCAGTGTG TGTTTGCAAT GAAAGTGTAA GGACTGTCAT TGTGCCTTCT 
GAGAAGGTTG TAGATTTGTT ATCCAATAGA AACAACCATA CAGGTCCTTC ACATAGAACT 
GAAGAAGTGA GGTATGGCGT GAGTGAGCAG ACCTCTTTAA AAACAGTGTC AAGAACCACA 
TCACCACCAT TATCAATTCC CACCACTCAT CTAATTCATC AGCCTGCAGG CTCCAGATCA 
CTGGAACCTT CTGGAATTTT ACTTAAGTCT GGAAATTACA GTGGACATTC TGATGGAATC 
TCAAGCAGCA GATCTCAAGC TGTGGAGGCT CCCTCTGTAT CTGTTAATCA CTATTCGCCA 
AATTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
AGACGATTGC ACATAGGTTG TCGGACAATA CAAGAAAGAT TAGATAGTTT AAGAGCAGCA 
TCTCAAAGCA CGACAGATTA TAACCAGGTC GTCCCCAACC GCACTACTTT GCAGGGACGA 
CGTCGAAGCA CCTCTCATGA TCGAGTGCCC CAGTCTGTCC AGATACGGCA ACGCAGTGTG 
TCCCAAGAAA GACTGGAAGA TTCTGTGCTA ATGAAGTATT GTCCAAGAAG TGCATCTCAA 
GGAGCACTGA CGTCTCCATC TGTTAGTTTT AGTAATCATA GAACTCGTTC ATGGGATTAT 
ATTGAGGGAC AGGATGAAAC CTTAGAAAAT GTCAATTCTG GAACTCCAAT ACCTGATTCC 
AATGGAGAGA AAAAACAGAC TTACAAGTGG AGTGGGTTTA CTGAACAGGA TGATAGACGA 
GGTATTTGTG AAAGACCTAG GCAGCAAGAA ATTCATAAAT CTTTTCGAGG TTCCAATTTT 
ACTGTGGCTC CAAGCGTTGT TAATTCTGAT AACAGGCGAA TGAGTGGTAG AGGAGTGGGA 
TCTGTGTCGC AGTTTAAAAA AATTCCACCA GATCTAAAAA CATTGCAGTC AAACAGAAAT 
TTTCAGACTA CTTGTGGAAT GTCACTGCCT CGGGGTATTT CACAAGACAG GTCACCTCTT 
GTGAAAGTCC GAAGTAATTC TCTGAAAGCT CCTTCCACGC ATGTCACAAA ACCATCATTT 
AGCCAGAAAT CATTTGTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAGAAC 
AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG ACTGACAGTG CCCCCGATCA GCAAGTGGAG 
ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA 
AACGCTGGTA CTTCAGATTT AGAACTACCT GTCAGTCAAA GGAATCAAGA TTTAAGTTTA 
CAAGAGGCTG AAACTGAGCA ATCAGATACT TTAGATAATA AAGAAGCTGT CATCCTAAGG 
GAAAAACCTC CATCTGGACG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTG 
GCAGTAAATG ACCAGGAGAC CGGGTCAGAC ACTACCTGCT GGCTGCCCAA TGATGCACGT 
CGAGAGGTCC ACATAAAAAG AATGGAGGAA AGAAAAGCCT CGAGTACCAG TCCGCCTGGC 
GATTCTTTGG CTTCCATCCC ATTTATAGTA AAAGGAATTT ATTATAAAGA ATGCAACAAT 
ACTGAAATGA GTAAGCCCAA TGTTAGTCTC TTCTCCCACG CCTTCTCCAT TCAGTCCTCC 
TTTAGAACAG ATGAACCAAC TAGCCCTAGC ATTGATCATG ATATTGCACA TATCCCTGCC 
TCTGCTGTTA TATCAGCCTC TACCTCTCAG GTCCCCTCCA TAGCAACAGT TCCTCCTTGC 
CTCACAACTT CAGCTCCATT AATTCGCCGT CAGCTCTCAC ATGACCACGA ATCTGTTGGC 
CCTCCTAGCC TGGATGCTCA GCCCAACTCA AAGACAGAAA GATCAAAATC ATATGATGAG 
GGTCTGGATG ATTACAGAGA AGATGCAAAA TTGTCCTTTA AGCACGTATC TAGTCTGAAG 
GGAATCAAGA TCGCAGACAG CCAAAAGTCA TCAGAAGACT CTGGGTCCAG AAAAGATTCT 
TCCTCAGAGG TCTTCAGTGA TGCTGCCAAG GAAGGGTGGC TTCATTTCCG ACCCCTTGTC 
ACCGATAAGG GCAAGCGAGT TGGTGGAAGT ATTCGGCCAT GGAAACAGAT GTATGTTGTC 
CTTCGGGGTC ATTCACTTTA CCTGTACAAA GATAAAAGAG AGCAGACGAC TCCGTCTGAG 
GAAGAGCAGC CCATCAGTGT TAATGCTTGC TTGATAGACA TCTCTTACAG TGAGACCAAG 
AGGAAAAATG TGTTTCGACT CACCACGTCC GACTGTGAAT GCCTGTTTCA GGCTGAAGAC 
AGAGATGATA TGCTAGCTTG GATCAAGACG ATCCAGGAGA GCAGCAACCT AAACGAAGAG 
GACACTGGAG TCACTAACAG GGATCTAATT AGTCGAAGAA TAAAAGAATA CAACAATCTG 
ATGAGCAAAG CAGAACAGTT GCCAAAAACA CCTCGCCAGA GTCTCAGCAT CAGGCAAACT 
TTGCTTGGTG CTAAATCAGA GCCAAAGACT CAAAGCCCAC ACTCTCCGAA GGAAGAGTCG 
GAAAGGAAAC TTCTCAGTAA AGATGATACC AGTCCCCCAA AAGACAAAGG CACATGGAGA 
AAAGGCATTC CAAGTATCAT GAGAAAGACA TTTGAGAAAA AGCCAACTGC TACAGGAACT 
TTCGGCGTCC GACTAGATGA CTGCCCACCA GCTCATACTA ATCGGTATAT TCCATTAATA 
GTTGACATAT GTTGCAAATT AGTTGAAGAA AGAGGTCTTG AATATACAGG TATTTATAGA 
GTTCCTGGAA ATAATGCAGC CATCTCAAGT ATGCAAGAAG AACTCAACAA GGGAATGGCT 
GATATTGATA TACAAGATGA TAAATGGCGA GATTTGAATG TGATAAGCAG TTTACTAAAA 
TCCTTCTTCA GAAAACTCCC TGAGCCTCTC TTCACAAATG ATAAATATGC TGATTTTATT 
GAAGCCAATC GTAAAGAAGA TCCTCTAGAT CGTCTGAAAA CATTAAAAAG ACTAATTCAC 
GATTTGCCTG AACATCATTA TGAAACACTT AAGTTCCTTT CAGCTCATCT GAAGACAGTG 
GCAGAAAATT CAGAAAAAAA TAAGATGGAA CCAAGAAACC TAGCAATAGT GTTTGGTCCC 
ACCCTTGTTC GAACATCAGA AGACAACATG ACCCACATGG TCACCCACAT GCCTGACCAG 
TACAAGATTG TAGAAACGCT CATCCAGCAC CATGACTGGT TTTTCACAGA AGAAGGTGCT 
GAAGAGCCTC TTACAACAGT GCAGGAGGAA AGCACAGTAG ACTCCCAGCC AGTGCCAAAC 
ATAGATCATT TACTCACCAA CATTGGAAGG ACAGGAGTCT CCCCAGGAGA TGTATCAGAT 
TCAGCTACTA GTGACTCAAC AAAATCTAAG GGTTCTTGGG GATCTGGAAA GGATCAGTAT 
AGCAGGGAAC TGCTTGTGTC CTCCATCTTT GCAGCTGCTA GTCGCAAGAG GAAGAAGCCG 
AAAGAAAAAG CACAGCCTAG CAGCTCAGAA GATGAACTGG ACAATGTATT TTTTAAGAAA 
GAAAATGTGG AACAGTGTCA CAATGATACT AAAGAGGAGT CCAAAAAAGA AAGTGAGACA 
CTGGGCAGAA AACAGAAGAT CATCATTGCC AAAGAAAACA GCACTAGGAA AGACCCCAGC 
ACGACAAAAG ATGAAAAGAT ATCACTAGGA AAAGAGAGCA CGCCTTCTGA AGAACCCTCA 
CCACCACACA ACTCAAAACA CAACAAGTCA CCAACTCTCA GCTGTCGCTT TGCCATCCTG 
AAAGAGAGCC CCAGGTCACT TCTGGCACAG AAGTCCTCCC ACCTTGAAGA GACAGGCTCT 
GACTCTGGCA CTTTGCTCAG CACGTCTTCC CAGGCCTCCC TGGCAAGGTT TTCCATGAAG 
AAATCAACCA GTCCAGAAAC GAAACATAGC GAGTTTTTGG CCAACGTCAG CACCATCACC 
TCAGATTATT CCACCACATC GTCTGCTACA TACTTGACTA GCCTGGACTC CAGTCGACTG 
AGCCCTGAGG TGCAATCCGT GGCAGAGAGC AAGGGGGACG AGGCAGATGA CGAGAGAAGC 
GAACTCATCA GTGAAGGGCG GCCTGTGGAA ACCGACAGCG AGAGCGAGTT TCCCGTGTTC 
CCCACAGCCT TGACTTCAGA GAGGCTTTTC CGAGGAAAAC TGCAAGAAGT GACTAAGAGC 
AGCCGGAGAA ATTCTGAAGG AAGTGAATTA AGTTGCACCG AGGGAAGTTT AACATCAAGT 
TTAGATAGCC GGAGACAGCT CTTCAGTTCC CATAAACTCA TCGAATGTGA TACTCTTTCC 
AGGAAAAAAT CAGCTAGATT CAAGTCAGAT AGTGGAAGTC TAGGAGATGC CAAGAATGAG 
AAAGAAGCAC CTTCGTTAAC TAAAGTGTTT GATGTTATGA AAAAAGGAAA GTCAACTGGG 
AGTTTACTGA CACCCACCAG AGGCGAATCC GAAAAACAGG AACCCACATG GAAAACGAAA 
ATAGCAGATC GGTTAAAACT GAGACCCAGA GCCCCTGCGG ATGACATGTT TGGAGTAGGG 
AATCACAAAG TGAATGCCGA GACTGCTAAA AGGAAAAGCA TCCGGCGCAG ACATACACTA 
GGAGGGCACA GAGATGCTAC CGAAATCAGC GTTTTGAATT TTTGGAAAGT GCATGAGCAG 
AGCGGGGAGA GAGAATCTGA ACTTTCAGCT GTAAACCGGT TAAAACCAAA ATGCTCAGCC 
CAGGACCTTT CCATCTCAGA CTGGCTGGCC AGGGAACGCC TACGCACCAG TACCTCTGAC 
CTTAGCAGAG GAGAAATCGG AGATCCCCAG ACAGAGAACC CAAGCACACG AGAAATAGCC 
ACGACCGACA CACCTTTGTC TCTTCATTGC AACACAGGCA GTTCTTCCAG CACCTTGGCT 
TCAACAAACA GGCCCCTTCT TTCCATACCA CCACAGTCAC CTGACCAAAT AAACGGAGAA 
AGCTTCCAGA ACGTGAGCAA AAATGCTAGT TCTGCAGCGA ATGCCCAACC TCATAAACTG 
TCTGAAACCC CAGGCAGTAA AGCAGAGTTT CATCCCTGTC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303856.1
CDS	297..6041
Translation	MEALCSWSVSQPLLWKQETPQGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_024448088.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448088.1

ORF Insert Sequence:

ATGGAAGCTC TGTGCAGCTG GTCAGTCTCT CAGCCATTAC TTTGGAAACA GGAAACGCCT 
CAAGGAAAAC AAAGAAACCG CTTGGAACCA ATGGATACCA TATTTGTTAA GCAAGTTAAA 
GAAGGAGGAC CTGCTTTTGA AGCTGGATTA TGTACAGGTG ACCGAATTAT AAAAGTCAAT 
GGAGAAAGTG TTATTGGCAA AACCTATTCC CAAGTAATTG CTTTAATTCA AAACAGTGAT 
ACAACATTGG AACTTAGTGT TATGCCAAAA GATGAAGACA TTCTCCAAGT GGCATATTCT 
CAAGATGCCT ACCTGAAAGG CAACGAAGCT TATAGCGGCA ATGCCCGCAA TATACCTGAA 
CCTCCACCAA TCTGCTATCC CTGGCTGCCA TCTGCCCCAT CAGCCATGGC ACAGCCAGTT 
GAAATATCTC CTCCTGACTC ATCATTGAGC AAACAGCAAA CCAGTACACC AGTACTGACA 
CAACCTGGTA GGGCCTATAG AATGGAAATA CAAGTGCCTC CATCACCAAC AGATGTTGCA 
AAATCAAACA CAGCAGTGTG TGTTTGCAAT GAAAGTGTAA GGACTGTCAT TGTGCCTTCT 
GAGAAGGTTG TAGATTTGTT ATCCAATAGA AACAACCATA CAGGTCCTTC ACATAGAACT 
GAAGAAGTGA GGTATGGCGT GAGTGAGCAG ACCTCTTTAA AAACAGTGTC AAGAACCACA 
TCACCACCAT TATCAATTCC CACCACTCAT CTAATTCATC AGCCTGCAGG CTCCAGATCA 
CTGGAACCTT CTGGAATTTT ACTTAAGTCT GGAAATTACA GTGGACATTC TGATGGAATC 
TCAAGCAGCA GATCTCAAGC TGTGGAGGCT CCCTCTGTAT CTGTTAATCA CTATTCGCCA 
AATTCCCATC AGCACATAGA CTGGAAAAAC TATAAAACTT ACAAAGAGTA TATTGATAAC 
AGACGATTGC ACATAGGTTG TCGGACAATA CAAGAAAGAT TAGATAGTTT AAGAGCAGCA 
TCTCAAAGCA CGACAGATTA TAACCAGGTC GTCCCCAACC GCACTACTTT GCAGGGACGA 
CGTCGAAGCA CCTCTCATGA TCGAGTGCCC CAGTCTGTCC AGATACGGCA ACGCAGTGTG 
TCCCAAGAAA GACTGGAAGA TTCTGTGCTA ATGAAGTATT GTCCAAGAAG TGCATCTCAA 
GGAGCACTGA CGTCTCCATC TGTTAGTTTT AGTAATCATA GAACTCGTTC ATGGGATTAT 
ATTGAGGGAC AGGATGAAAC CTTAGAAAAT GTCAATTCTG GAACTCCAAT ACCTGATTCC 
AATGGAGAGA AAAAACAGAC TTACAAGTGG AGTGGGTTTA CTGAACAGGA TGATAGACGA 
GGTATTTGTG AAAGACCTAG GCAGCAAGAA ATTCATAAAT CTTTTCGAGG TTCCAATTTT 
ACTGTGGCTC CAAGCGTTGT TAATTCTGAT AACAGGCGAA TGAGTGGTAG AGGAGTGGGA 
TCTGTGTCGC AGTTTAAAAA AATTCCACCA GATCTAAAAA CATTGCAGTC AAACAGAAAT 
TTTCAGACTA CTTGTGGAAT GTCACTGCCT CGGGGTATTT CACAAGACAG GTCACCTCTT 
GTGAAAGTCC GAAGTAATTC TCTGAAAGCT CCTTCCACGC ATGTCACAAA ACCATCATTT 
AGCCAGAAAT CATTTGTTTC TATCAAAGAC CAAAGACCAG TAAATCACTT GCATCAGAAC 
AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG ACTGACAGTG CCCCCGATCA GCAAGTGGAG 
ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA 
AACGCTGGTA CTTCAGATTT AGAACTACCT GTCAGTCAAA GGAATCAAGA TTTAAGTTTA 
CAAGAGGCTG AAACTGAGCA ATCAGATACT TTAGATAATA AAGAAGCTGT CATCCTAAGG 
GAAAAACCTC CATCTGGACG CCAGACACCG CAGCCTTTAA GGCATCAGTC TTACATCTTG 
GCAGTAAATG ACCAGGAGAC CGGGTCAGAC ACTACCTGCT GGCTGCCCAA TGATGCACGT 
CGAGAGGTCC ACATAAAAAG AATGGAGGAA AGAAAAGCCT CGAGTACCAG TCCGCCTGGC 
GATTCTTTGG CTTCCATCCC ATTTATAGTA AAAGGAATTT ATTATAAAGA ATGCAACAAT 
ACTGAAATGA GTAAGCCCAA TGTTAGTCTC TTCTCCCACG CCTTCTCCAT TCAGTCCTCC 
TTTAGAACAG ATGAACCAAC TAGCCCTAGC ATTGATCATG ATATTGCACA TATCCCTGCC 
TCTGCTGTTA TATCAGCCTC TACCTCTCAG GTCCCCTCCA TAGCAACAGT TCCTCCTTGC 
CTCACAACTT CAGCTCCATT AATTCGCCGT CAGCTCTCAC ATGACCACGA ATCTGTTGGC 
CCTCCTAGCC TGGATGCTCA GCCCAACTCA AAGACAGAAA GATCAAAATC ATATGATGAG 
GGTCTGGATG ATTACAGAGA AGATGCAAAA TTGTCCTTTA AGCACGTATC TAGTCTGAAG 
GGAATCAAGA TCGCAGACAG CCAAAAGTCA TCAGAAGACT CTGGGTCCAG AAAAGATTCT 
TCCTCAGAGG TCTTCAGTGA TGCTGCCAAG GAAGGGTGGC TTCATTTCCG ACCCCTTGTC 
ACCGATAAGG GCAAGCGAGT TGGTGGAAGT ATTCGGCCAT GGAAACAGAT GTATGTTGTC 
CTTCGGGGTC ATTCACTTTA CCTGTACAAA GATAAAAGAG AGCAGACGAC TCCGTCTGAG 
GAAGAGCAGC CCATCAGTGT TAATGCTTGC TTGATAGACA TCTCTTACAG TGAGACCAAG 
AGGAAAAATG TGTTTCGACT CACCACGTCC GACTGTGAAT GCCTGTTTCA GGCTGAAGAC 
AGAGATGATA TGCTAGCTTG GATCAAGACG ATCCAGGAGA GCAGCAACCT AAACGAAGAG 
GACACTGGAG TCACTAACAG GGATCTAATT AGTCGAAGAA TAAAAGAATA CAACAATCTG 
ATGAGCAAAG CAGAACAGTT GCCAAAAACA CCTCGCCAGA GTCTCAGCAT CAGGCAAACT 
TTGCTTGGTG CTAAATCAGA GCCAAAGACT CAAAGCCCAC ACTCTCCGAA GGAAGAGTCG 
GAAAGGAAAC TTCTCAGTAA AGATGATACC AGTCCCCCAA AAGACAAAGG CACATGGAGA 
AAAGGCATTC CAAGTATCAT GAGAAAGACA TTTGAGAAAA AGCCAACTGC TACAGGAACT 
TTCGGCGTCC GACTAGATGA CTGCCCACCA GCTCATACTA ATCGGTATAT TCCATTAATA 
GTTGACATAT GTTGCAAATT AGTTGAAGAA AGAGGTCTTG AATATACAGG TATTTATAGA 
GTTCCTGGAA ATAATGCAGC CATCTCAAGT ATGCAAGAAG AACTCAACAA GGGAATGGCT 
GATATTGATA TACAAGATGA TAAATGGCGA GATTTGAATG TGATAAGCAG TTTACTAAAA 
TCCTTCTTCA GAAAACTCCC TGAGCCTCTC TTCACAAATG ATAAATATGC TGATTTTATT 
GAAGCCAATC GTAAAGAAGA TCCTCTAGAT CGTCTGAAAA CATTAAAAAG ACTAATTCAC 
GATTTGCCTG AACATCATTA TGAAACACTT AAGTTCCTTT CAGCTCATCT GAAGACAGTG 
GCAGAAAATT CAGAAAAAAA TAAGATGGAA CCAAGAAACC TAGCAATAGT GTTTGGTCCC 
ACCCTTGTTC GAACATCAGA AGACAACATG ACCCACATGG TCACCCACAT GCCTGACCAG 
TACAAGATTG TAGAAACGCT CATCCAGCAC CATGACTGGT TTTTCACAGA AGAAGGTGCT 
GAAGAGCCTC TTACAACAGT GCAGGAGGAA AGCACAGTAG ACTCCCAGCC AGTGCCAAAC 
ATAGATCATT TACTCACCAA CATTGGAAGG ACAGGAGTCT CCCCAGGAGA TGTATCAGAT 
TCAGCTACTA GTGACTCAAC AAAATCTAAG GGTTCTTGGG GATCTGGAAA GGATCAGTAT 
AGCAGGGAAC TGCTTGTGTC CTCCATCTTT GCAGCTGCTA GTCGCAAGAG GAAGAAGCCG 
AAAGAAAAAG CACAGCCTAG CAGCTCAGAA GATGAACTGG ACAATGTATT TTTTAAGAAA 
GAAAATGTGG AACAGTGTCA CAATGATACT AAAGAGGAGT CCAAAAAAGA AAGTGAGACA 
CTGGGCAGAA AACAGAAGAT CATCATTGCC AAAGAAAACA GCACTAGGAA AGACCCCAGC 
ACGACAAAAG ATGAAAAGAT ATCACTAGGA AAAGAGAGCA CGCCTTCTGA AGAACCCTCA 
CCACCACACA ACTCAAAACA CAACAAGTCA CCAACTCTCA GCTGTCGCTT TGCCATCCTG 
AAAGAGAGCC CCAGGTCACT TCTGGCACAG AAGTCCTCCC ACCTTGAAGA GACAGGCTCT 
GACTCTGGCA CTTTGCTCAG CACGTCTTCC CAGGCCTCCC TGGCAAGGTT TTCCATGAAG 
AAATCAACCA GTCCAGAAAC GAAACATAGC GAGTTTTTGG CCAACGTCAG CACCATCACC 
TCAGATTATT CCACCACATC GTCTGCTACA TACTTGACTA GCCTGGACTC CAGTCGACTG 
AGCCCTGAGG TGCAATCCGT GGCAGAGAGC AAGGGGGACG AGGCAGATGA CGAGAGAAGC 
GAACTCATCA GTGAAGGGCG GCCTGTGGAA ACCGACAGCG AGAGCGAGTT TCCCGTGTTC 
CCCACAGCCT TGACTTCAGA GAGGCTTTTC CGAGGAAAAC TGCAAGAAGT GACTAAGAGC 
AGCCGGAGAA ATTCTGAAGG AAGTGAATTA AGTTGCACCG AGGGAAGTTT AACATCAAGT 
TTAGATAGCC GGAGACAGCT CTTCAGTTCC CATAAACTCA TCGAATGTGA TACTCTTTCC 
AGGAAAAAAT CAGCTAGATT CAAGTCAGAT AGTGGAAGTC TAGGAGATGC CAAGAATGAG 
AAAGAAGCAC CTTCGTTAAC TAAAGTGTTT GATGTTATGA AAAAAGGAAA GTCAACTGGG 
AGTTTACTGA CACCCACCAG AGGCGAATCC GAAAAACAGG AACCCACATG GAAAACGAAA 
ATAGCAGATC GGTTAAAACT GAGACCCAGA GCCCCTGCGG ATGACATGTT TGGAGTAGGG 
AATCACAAAG TGAATGCCGA GACTGCTAAA AGGAAAAGCA TCCGGCGCAG ACATACACTA 
GGAGGGCACA GAGATGCTAC CGAAATCAGC GTTTTGAATT TTTGGAAAGT GCATGAGCAG 
AGCGGGGAGA GAGAATCTGA ACTTTCAGCT GTAAACCGGT TAAAACCAAA ATGCTCAGCC 
CAGGACCTTT CCATCTCAGA CTGGCTGGCC AGGGAACGCC TACGCACCAG TACCTCTGAC 
CTTAGCAGAG GAGAAATCGG AGATCCCCAG ACAGAGAACC CAAGCACACG AGAAATAGCC 
ACGACCGACA CACCTTTGTC TCTTCATTGC AACACAGGCA GTTCTTCCAG CACCTTGGCT 
TCAACAAACA GGCCCCTTCT TTCCATACCA CCACAGTCAC CTGACCAAAT AAACGGAGAA 
AGCTTCCAGA ACGTGAGCAA AAATGCTAGT TCTGCAGCGA ATGCCCAACC TCATAAACTG 
TCTGAAACCC CAGGCAGTAA AGCAGAGTTT CATCCCTGTC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367454.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354383.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 3, mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	NM_001367454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. Transcript Variant: This variant (3), as well as variants 1 and 2, encodes isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.213934.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354383.1
CDS	590..6466
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367454.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367454.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_020824.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065875.3 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	NM_020824.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AV709499.1, AF480466.1, AB037845.2, BI491305.1 and BQ026288.1. On Sep 17, 2008 this sequence version replaced NM_020824.2. Sequence Note: The 5'-most in-frame translation start codon is selected for this RefSeq and is well-conserved among mammalian species. An alternative start codon that would reduce the protein length by 1 aa is also present. The use of the downstream start codon is assumed in the literature, including PMIDs:12056806, 15793564 and 17347647. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF480466.1, SRR1803615.86871.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065875.3
CDS	488..6364
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_020824.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020824.3

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367448.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354377.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 2, mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	NM_001367448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. On Nov 30, 2018 this sequence version replaced XM_005252542.3. Transcript Variant: This variant (2), as well as variants 1 and 3, encodes isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.98046.1, SRR1803615.61494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354377.1
CDS	446..6322
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367448.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367448.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_020824.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065875.3 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 1, mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	NM_020824.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11, AV709499.1, AF480466.1, AB037845.2, BI491305.1 and BQ026288.1. On Nov 22, 2018 this sequence version replaced NM_020824.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: The 5'-most in-frame translation start codon is selected for this RefSeq and is well-conserved among mammalian species. An alternative start codon that would reduce the protein length by 1 aa is also present. The use of the downstream start codon is assumed in the literature, including PMIDs:12056806, 15793564 and 17347647. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF480466.1, SRR1803615.86871.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000396432.7/ ENSP00000379709.2 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065875.3
CDS	707..6583
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_020824.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020824.4

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367447.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354376.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 4, mRNA.

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CloneID	OHu43829
Clone ID Related Accession (Same CDS sequence)	NM_001367447.1 , NM_001367453.1 , XM_017016460.1 , XM_005252544.4 , NM_001367451.1 , XM_017016461.1
Accession Version	NM_001367447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. On Nov 30, 2018 this sequence version replaced XM_005252544.4. Transcript Variant: This variant (4), as well as variants 5 and 6, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.252435.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354376.1
CDS	707..6553
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367447.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367447.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367453.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354382.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 6, mRNA.

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CloneID	OHu43829
Clone ID Related Accession (Same CDS sequence)	NM_001367447.1 , NM_001367453.1 , XM_017016460.1 , XM_005252544.4 , NM_001367451.1 , XM_017016461.1
Accession Version	NM_001367453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. On Nov 30, 2018 this sequence version replaced XM_017016460.2. Transcript Variant: This variant (6), as well as variants 4 and 5, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.160280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354382.1
CDS	644..6490
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367453.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367453.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( NM_001367451.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354380.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 5, mRNA.

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CloneID	OHu43829
Clone ID Related Accession (Same CDS sequence)	NM_001367447.1 , NM_001367453.1 , XM_017016460.1 , XM_005252544.4 , NM_001367451.1 , XM_017016461.1
Accession Version	NM_001367451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355979.11 and AL392104.19. On Nov 30, 2018 this sequence version replaced XM_017016461.1. Transcript Variant: This variant (5), as well as variants 4 and 6, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.231187.1, SRR1803612.126703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354380.1
CDS	446..6292
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	NM_001367451.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367451.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005252542.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252599.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	XM_005252542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252542.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252599.1
CDS	556..6432
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_005252542.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252542.3

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_017016459.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871948.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	XM_017016459.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871948.1
CDS	648..6524
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_017016459.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016459.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_017016459.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871948.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

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CloneID	OHu03540
Clone ID Related Accession (Same CDS sequence)	XM_005252542.3 , NM_001367454.1 , XM_017016459.1 , NM_020824.3 , XM_017016459.2 , NM_001367448.1 , NM_020824.4
Accession Version	XM_017016459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871948.1
CDS	656..6532
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_017016459.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016459.2

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGATGAACCA ACTAGCCCTA GCATTGATCA TGATATTGCA 
CATATCCCTG CCTCTGCTGT TATATCAGCC TCTACCTCTC AGGTCCCCTC CATAGCAACA 
GTTCCTCCTT GCCTCACAAC TTCAGCTCCA TTAATTCGCC GTCAGCTCTC ACATGACCAC 
GAATCTGTTG GCCCTCCTAG CCTGGATGCT CAGCCCAACT CAAAGACAGA AAGATCAAAA 
TCATATGATG AGGGTCTGGA TGATTACAGA GAAGATGCAA AATTGTCCTT TAAGCACGTA 
TCTAGTCTGA AGGGAATCAA GATCGCAGAC AGCCAAAAGT CATCAGAAGA CTCTGGGTCC 
AGAAAAGATT CTTCCTCAGA GGTCTTCAGT GATGCTGCCA AGGAAGGGTG GCTTCATTTC 
CGACCCCTTG TCACCGATAA GGGCAAGCGA GTTGGTGGAA GTATTCGGCC ATGGAAACAG 
ATGTATGTTG TCCTTCGGGG TCATTCACTT TACCTGTACA AAGATAAAAG AGAGCAGACG 
ACTCCGTCTG AGGAAGAGCA GCCCATCAGT GTTAATGCTT GCTTGATAGA CATCTCTTAC 
AGTGAGACCA AGAGGAAAAA TGTGTTTCGA CTCACCACGT CCGACTGTGA ATGCCTGTTT 
CAGGCTGAAG ACAGAGATGA TATGCTAGCT TGGATCAAGA CGATCCAGGA GAGCAGCAAC 
CTAAACGAAG AGGACACTGG AGTCACTAAC AGGGATCTAA TTAGTCGAAG AATAAAAGAA 
TACAACAATC TGATGAGCAA AGCAGAACAG TTGCCAAAAA CACCTCGCCA GAGTCTCAGC 
ATCAGGCAAA CTTTGCTTGG TGCTAAATCA GAGCCAAAGA CTCAAAGCCC ACACTCTCCG 
AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT AAAGATGATA CCAGTCCCCC AAAAGACAAA 
GGCACATGGA GAAAAGGCAT TCCAAGTATC ATGAGAAAGA CATTTGAGAA AAAGCCAACT 
GCTACAGGAA CTTTCGGCGT CCGACTAGAT GACTGCCCAC CAGCTCATAC TAATCGGTAT 
ATTCCATTAA TAGTTGACAT ATGTTGCAAA TTAGTTGAAG AAAGAGGTCT TGAATATACA 
GGTATTTATA GAGTTCCTGG AAATAATGCA GCCATCTCAA GTATGCAAGA AGAACTCAAC 
AAGGGAATGG CTGATATTGA TATACAAGAT GATAAATGGC GAGATTTGAA TGTGATAAGC 
AGTTTACTAA AATCCTTCTT CAGAAAACTC CCTGAGCCTC TCTTCACAAA TGATAAATAT 
GCTGATTTTA TTGAAGCCAA TCGTAAAGAA GATCCTCTAG ATCGTCTGAA AACATTAAAA 
AGACTAATTC ACGATTTGCC TGAACATCAT TATGAAACAC TTAAGTTCCT TTCAGCTCAT 
CTGAAGACAG TGGCAGAAAA TTCAGAAAAA AATAAGATGG AACCAAGAAA CCTAGCAATA 
GTGTTTGGTC CCACCCTTGT TCGAACATCA GAAGACAACA TGACCCACAT GGTCACCCAC 
ATGCCTGACC AGTACAAGAT TGTAGAAACG CTCATCCAGC ACCATGACTG GTTTTTCACA 
GAAGAAGGTG CTGAAGAGCC TCTTACAACA GTGCAGGAGG AAAGCACAGT AGACTCCCAG 
CCAGTGCCAA ACATAGATCA TTTACTCACC AACATTGGAA GGACAGGAGT CTCCCCAGGA 
GATGTATCAG ATTCAGCTAC TAGTGACTCA ACAAAATCTA AGGGTTCTTG GGGATCTGGA 
AAGGATCAGT ATAGCAGGGA ACTGCTTGTG TCCTCCATCT TTGCAGCTGC TAGTCGCAAG 
AGGAAGAAGC CGAAAGAAAA AGCACAGCCT AGCAGCTCAG AAGATGAACT GGACAATGTA 
TTTTTTAAGA AAGAAAATGT GGAACAGTGT CACAATGATA CTAAAGAGGA GTCCAAAAAA 
GAAAGTGAGA CACTGGGCAG AAAACAGAAG ATCATCATTG CCAAAGAAAA CAGCACTAGG 
AAAGACCCCA GCACGACAAA AGATGAAAAG ATATCACTAG GAAAAGAGAG CACGCCTTCT 
GAAGAACCCT CACCACCACA CAACTCAAAA CACAACAAGT CACCAACTCT CAGCTGTCGC 
TTTGCCATCC TGAAAGAGAG CCCCAGGTCA CTTCTGGCAC AGAAGTCCTC CCACCTTGAA 
GAGACAGGCT CTGACTCTGG CACTTTGCTC AGCACGTCTT CCCAGGCCTC CCTGGCAAGG 
TTTTCCATGA AGAAATCAAC CAGTCCAGAA ACGAAACATA GCGAGTTTTT GGCCAACGTC 
AGCACCATCA CCTCAGATTA TTCCACCACA TCGTCTGCTA CATACTTGAC TAGCCTGGAC 
TCCAGTCGAC TGAGCCCTGA GGTGCAATCC GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT 
GACGAGAGAA GCGAACTCAT CAGTGAAGGG CGGCCTGTGG AAACCGACAG CGAGAGCGAG 
TTTCCCGTGT TCCCCACAGC CTTGACTTCA GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA 
GTGACTAAGA GCAGCCGGAG AAATTCTGAA GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT 
TTAACATCAA GTTTAGATAG CCGGAGACAG CTCTTCAGTT CCCATAAACT CATCGAATGT 
GATACTCTTT CCAGGAAAAA ATCAGCTAGA TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT 
GCCAAGAATG AGAAAGAAGC ACCTTCGTTA ACTAAAGTGT TTGATGTTAT GAAAAAAGGA 
AAGTCAACTG GGAGTTTACT GACACCCACC AGAGGCGAAT CCGAAAAACA GGAACCCACA 
TGGAAAACGA AAATAGCAGA TCGGTTAAAA CTGAGACCCA GAGCCCCTGC GGATGACATG 
TTTGGAGTAG GGAATCACAA AGTGAATGCC GAGACTGCTA AAAGGAAAAG CATCCGGCGC 
AGACATACAC TAGGAGGGCA CAGAGATGCT ACCGAAATCA GCGTTTTGAA TTTTTGGAAA 
GTGCATGAGC AGAGCGGGGA GAGAGAATCT GAACTTTCAG CTGTAAACCG GTTAAAACCA 
AAATGCTCAG CCCAGGACCT TTCCATCTCA GACTGGCTGG CCAGGGAACG CCTACGCACC 
AGTACCTCTG ACCTTAGCAG AGGAGAAATC GGAGATCCCC AGACAGAGAA CCCAAGCACA 
CGAGAAATAG CCACGACCGA CACACCTTTG TCTCTTCATT GCAACACAGG CAGTTCTTCC 
AGCACCTTGG CTTCAACAAA CAGGCCCCTT CTTTCCATAC CACCACAGTC ACCTGACCAA 
ATAAACGGAG AAAGCTTCCA GAACGTGAGC AAAAATGCTA GTTCTGCAGC GAATGCCCAA 
CCTCATAAAC TGTCTGAAAC CCCAGGCAGT AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_017016460.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871949.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu43829
Clone ID Related Accession (Same CDS sequence)	NM_001367447.1 , NM_001367453.1 , XM_017016460.1 , XM_005252544.4 , NM_001367451.1 , XM_017016461.1
Accession Version	XM_017016460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871949.1
CDS	648..6494
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_017016460.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016460.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_005252544.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252601.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.

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CloneID	OHu43829
Clone ID Related Accession (Same CDS sequence)	NM_001367447.1 , NM_001367453.1 , XM_017016460.1 , XM_005252544.4 , NM_001367451.1 , XM_017016461.1
Accession Version	XM_005252544.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252544.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252601.1
CDS	2241..8087
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_005252544.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252544.4

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_017016461.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871950.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X9, mRNA.

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CloneID	OHu43829
Clone ID Related Accession (Same CDS sequence)	NM_001367447.1 , NM_001367453.1 , XM_017016460.1 , XM_005252544.4 , NM_001367451.1 , XM_017016461.1
Accession Version	XM_017016461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871950.1
CDS	459..6305
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_017016461.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016461.1

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGATGAACCA 
ACTAGCCCTA GCATTGATCA TGATATTGCA CATATCCCTG CCTCTGCTGT TATATCAGCC 
TCTACCTCTC AGGTCCCCTC CATAGCAACA GTTCCTCCTT GCCTCACAAC TTCAGCTCCA 
TTAATTCGCC GTCAGCTCTC ACATGACCAC GAATCTGTTG GCCCTCCTAG CCTGGATGCT 
CAGCCCAACT CAAAGACAGA AAGATCAAAA TCATATGATG AGGGTCTGGA TGATTACAGA 
GAAGATGCAA AATTGTCCTT TAAGCACGTA TCTAGTCTGA AGGGAATCAA GATCGCAGAC 
AGCCAAAAGT CATCAGAAGA CTCTGGGTCC AGAAAAGATT CTTCCTCAGA GGTCTTCAGT 
GATGCTGCCA AGGAAGGGTG GCTTCATTTC CGACCCCTTG TCACCGATAA GGGCAAGCGA 
GTTGGTGGAA GTATTCGGCC ATGGAAACAG ATGTATGTTG TCCTTCGGGG TCATTCACTT 
TACCTGTACA AAGATAAAAG AGAGCAGACG ACTCCGTCTG AGGAAGAGCA GCCCATCAGT 
GTTAATGCTT GCTTGATAGA CATCTCTTAC AGTGAGACCA AGAGGAAAAA TGTGTTTCGA 
CTCACCACGT CCGACTGTGA ATGCCTGTTT CAGGCTGAAG ACAGAGATGA TATGCTAGCT 
TGGATCAAGA CGATCCAGGA GAGCAGCAAC CTAAACGAAG AGGACACTGG AGTCACTAAC 
AGGGATCTAA TTAGTCGAAG AATAAAAGAA TACAACAATC TGATGAGCAA AGCAGAACAG 
TTGCCAAAAA CACCTCGCCA GAGTCTCAGC ATCAGGCAAA CTTTGCTTGG TGCTAAATCA 
GAGCCAAAGA CTCAAAGCCC ACACTCTCCG AAGGAAGAGT CGGAAAGGAA ACTTCTCAGT 
AAAGATGATA CCAGTCCCCC AAAAGACAAA GGCACATGGA GAAAAGGCAT TCCAAGTATC 
ATGAGAAAGA CATTTGAGAA AAAGCCAACT GCTACAGGAA CTTTCGGCGT CCGACTAGAT 
GACTGCCCAC CAGCTCATAC TAATCGGTAT ATTCCATTAA TAGTTGACAT ATGTTGCAAA 
TTAGTTGAAG AAAGAGGTCT TGAATATACA GGTATTTATA GAGTTCCTGG AAATAATGCA 
GCCATCTCAA GTATGCAAGA AGAACTCAAC AAGGGAATGG CTGATATTGA TATACAAGAT 
GATAAATGGC GAGATTTGAA TGTGATAAGC AGTTTACTAA AATCCTTCTT CAGAAAACTC 
CCTGAGCCTC TCTTCACAAA TGATAAATAT GCTGATTTTA TTGAAGCCAA TCGTAAAGAA 
GATCCTCTAG ATCGTCTGAA AACATTAAAA AGACTAATTC ACGATTTGCC TGAACATCAT 
TATGAAACAC TTAAGTTCCT TTCAGCTCAT CTGAAGACAG TGGCAGAAAA TTCAGAAAAA 
AATAAGATGG AACCAAGAAA CCTAGCAATA GTGTTTGGTC CCACCCTTGT TCGAACATCA 
GAAGACAACA TGACCCACAT GGTCACCCAC ATGCCTGACC AGTACAAGAT TGTAGAAACG 
CTCATCCAGC ACCATGACTG GTTTTTCACA GAAGAAGGTG CTGAAGAGCC TCTTACAACA 
GTGCAGGAGG AAAGCACAGT AGACTCCCAG CCAGTGCCAA ACATAGATCA TTTACTCACC 
AACATTGGAA GGACAGGAGT CTCCCCAGGA GATGTATCAG ATTCAGCTAC TAGTGACTCA 
ACAAAATCTA AGGGTTCTTG GGGATCTGGA AAGGATCAGT ATAGCAGGGA ACTGCTTGTG 
TCCTCCATCT TTGCAGCTGC TAGTCGCAAG AGGAAGAAGC CGAAAGAAAA AGCACAGCCT 
AGCAGCTCAG AAGATGAACT GGACAATGTA TTTTTTAAGA AAGAAAATGT GGAACAGTGT 
CACAATGATA CTAAAGAGGA GTCCAAAAAA GAAAGTGAGA CACTGGGCAG AAAACAGAAG 
ATCATCATTG CCAAAGAAAA CAGCACTAGG AAAGACCCCA GCACGACAAA AGATGAAAAG 
ATATCACTAG GAAAAGAGAG CACGCCTTCT GAAGAACCCT CACCACCACA CAACTCAAAA 
CACAACAAGT CACCAACTCT CAGCTGTCGC TTTGCCATCC TGAAAGAGAG CCCCAGGTCA 
CTTCTGGCAC AGAAGTCCTC CCACCTTGAA GAGACAGGCT CTGACTCTGG CACTTTGCTC 
AGCACGTCTT CCCAGGCCTC CCTGGCAAGG TTTTCCATGA AGAAATCAAC CAGTCCAGAA 
ACGAAACATA GCGAGTTTTT GGCCAACGTC AGCACCATCA CCTCAGATTA TTCCACCACA 
TCGTCTGCTA CATACTTGAC TAGCCTGGAC TCCAGTCGAC TGAGCCCTGA GGTGCAATCC 
GTGGCAGAGA GCAAGGGGGA CGAGGCAGAT GACGAGAGAA GCGAACTCAT CAGTGAAGGG 
CGGCCTGTGG AAACCGACAG CGAGAGCGAG TTTCCCGTGT TCCCCACAGC CTTGACTTCA 
GAGAGGCTTT TCCGAGGAAA ACTGCAAGAA GTGACTAAGA GCAGCCGGAG AAATTCTGAA 
GGAAGTGAAT TAAGTTGCAC CGAGGGAAGT TTAACATCAA GTTTAGATAG CCGGAGACAG 
CTCTTCAGTT CCCATAAACT CATCGAATGT GATACTCTTT CCAGGAAAAA ATCAGCTAGA 
TTCAAGTCAG ATAGTGGAAG TCTAGGAGAT GCCAAGAATG AGAAAGAAGC ACCTTCGTTA 
ACTAAAGTGT TTGATGTTAT GAAAAAAGGA AAGTCAACTG GGAGTTTACT GACACCCACC 
AGAGGCGAAT CCGAAAAACA GGAACCCACA TGGAAAACGA AAATAGCAGA TCGGTTAAAA 
CTGAGACCCA GAGCCCCTGC GGATGACATG TTTGGAGTAG GGAATCACAA AGTGAATGCC 
GAGACTGCTA AAAGGAAAAG CATCCGGCGC AGACATACAC TAGGAGGGCA CAGAGATGCT 
ACCGAAATCA GCGTTTTGAA TTTTTGGAAA GTGCATGAGC AGAGCGGGGA GAGAGAATCT 
GAACTTTCAG CTGTAAACCG GTTAAAACCA AAATGCTCAG CCCAGGACCT TTCCATCTCA 
GACTGGCTGG CCAGGGAACG CCTACGCACC AGTACCTCTG ACCTTAGCAG AGGAGAAATC 
GGAGATCCCC AGACAGAGAA CCCAAGCACA CGAGAAATAG CCACGACCGA CACACCTTTG 
TCTCTTCATT GCAACACAGG CAGTTCTTCC AGCACCTTGG CTTCAACAAA CAGGCCCCTT 
CTTTCCATAC CACCACAGTC ACCTGACCAA ATAAACGGAG AAAGCTTCCA GAACGTGAGC 
AAAAATGCTA GTTCTGCAGC GAATGCCCAA CCTCATAAAC TGTCTGAAAC CCCAGGCAGT 
AAAGCAGAGT TTCATCCCTG TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519602.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517904.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

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CloneID	OHu70669
Clone ID Related Accession (Same CDS sequence)	XM_011519602.2 , XM_011519603.3 , XM_011519603.2 , XM_011519604.2
Accession Version	XM_011519602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517904.1
CDS	2241..8219
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_011519602.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519602.2

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519603.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517905.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

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CloneID	OHu70669
Clone ID Related Accession (Same CDS sequence)	XM_011519602.2 , XM_011519603.3 , XM_011519603.2 , XM_011519604.2
Accession Version	XM_011519603.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519603.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517905.1
CDS	656..6634
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_011519603.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519603.3

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519603.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517905.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

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CloneID	OHu70669
Clone ID Related Accession (Same CDS sequence)	XM_011519602.2 , XM_011519603.3 , XM_011519603.2 , XM_011519604.2
Accession Version	XM_011519603.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517905.1
CDS	648..6626
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_011519603.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519603.2

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519604.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517906.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

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CloneID	OHu70669
Clone ID Related Accession (Same CDS sequence)	XM_011519602.2 , XM_011519603.3 , XM_011519603.2 , XM_011519604.2
Accession Version	XM_011519604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517906.1
CDS	464..6442
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_011519604.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519604.2

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTACAA CAGTGCAGGA GGAAAGCACA GTAGACTCCC AGCCAGTGCC AAACATAGAT 
CATTTACTCA CCAACATTGG AAGGACAGGA GTCTCCCCAG GAGATGTATC AGATTCAGCT 
ACTAGTGACT CAACAAAATC TAAGGGTTCT TGGGGATCTG GAAAGGATCA GTATAGCAGG 
GAACTGCTTG TGTCCTCCAT CTTTGCAGCT GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA 
AAAGCACAGC CTAGCAGCTC AGAAGATGAA CTGGACAATG TATTTTTTAA GAAAGAAAAT 
GTGGAACAGT GTCACAATGA TACTAAAGAG GAGTCCAAAA AAGAAAGTGA GACACTGGGC 
AGAAAACAGA AGATCATCAT TGCCAAAGAA AACAGCACTA GGAAAGACCC CAGCACGACA 
AAAGATGAAA AGATATCACT AGGAAAAGAG AGCACGCCTT CTGAAGAACC CTCACCACCA 
CACAACTCAA AACACAACAA GTCACCAACT CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG 
AGCCCCAGGT CACTTCTGGC ACAGAAGTCC TCCCACCTTG AAGAGACAGG CTCTGACTCT 
GGCACTTTGC TCAGCACGTC TTCCCAGGCC TCCCTGGCAA GGTTTTCCAT GAAGAAATCA 
ACCAGTCCAG AAACGAAACA TAGCGAGTTT TTGGCCAACG TCAGCACCAT CACCTCAGAT 
TATTCCACCA CATCGTCTGC TACATACTTG ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT 
GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG GACGAGGCAG ATGACGAGAG AAGCGAACTC 
ATCAGTGAAG GGCGGCCTGT GGAAACCGAC AGCGAGAGCG AGTTTCCCGT GTTCCCCACA 
GCCTTGACTT CAGAGAGGCT TTTCCGAGGA AAACTGCAAG AAGTGACTAA GAGCAGCCGG 
AGAAATTCTG AAGGAAGTGA ATTAAGTTGC ACCGAGGGAA GTTTAACATC AAGTTTAGAT 
AGCCGGAGAC AGCTCTTCAG TTCCCATAAA CTCATCGAAT GTGATACTCT TTCCAGGAAA 
AAATCAGCTA GATTCAAGTC AGATAGTGGA AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA 
GCACCTTCGT TAACTAAAGT GTTTGATGTT ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA 
CTGACACCCA CCAGAGGCGA ATCCGAAAAA CAGGAACCCA CATGGAAAAC GAAAATAGCA 
GATCGGTTAA AACTGAGACC CAGAGCCCCT GCGGATGACA TGTTTGGAGT AGGGAATCAC 
AAAGTGAATG CCGAGACTGC TAAAAGGAAA AGCATCCGGC GCAGACATAC ACTAGGAGGG 
CACAGAGATG CTACCGAAAT CAGCGTTTTG AATTTTTGGA AAGTGCATGA GCAGAGCGGG 
GAGAGAGAAT CTGAACTTTC AGCTGTAAAC CGGTTAAAAC CAAAATGCTC AGCCCAGGAC 
CTTTCCATCT CAGACTGGCT GGCCAGGGAA CGCCTACGCA CCAGTACCTC TGACCTTAGC 
AGAGGAGAAA TCGGAGATCC CCAGACAGAG AACCCAAGCA CACGAGAAAT AGCCACGACC 
GACACACCTT TGTCTCTTCA TTGCAACACA GGCAGTTCTT CCAGCACCTT GGCTTCAACA 
AACAGGCCCC TTCTTTCCAT ACCACCACAG TCACCTGACC AAATAAACGG AGAAAGCTTC 
CAGAACGTGA GCAAAAATGC TAGTTCTGCA GCGAATGCCC AACCTCATAA ACTGTCTGAA 
ACCCCAGGCA GTAAAGCAGA GTTTCATCCC TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517907.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

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CloneID	OHu70670
Clone ID Related Accession (Same CDS sequence)	XM_011519605.2
Accession Version	XM_011519605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGTAAAAGGA 
ATTTATTATA AAGAATGCAA CAATACTGAA ATGAGTAAGC CCAATGTTAG TCTCTTCTCC 
CACGCCTTCT CCATTCAGTC CTCCTTTAGA ACAGATGAAC CAACTAGCCC TAGCATTGAT 
CATGATATTG CACATATCCC TGCCTCTGCT GTTATATCAG CCTCTACCTC TCAGGTCCCC 
TCCATAGCAA CAGTTCCTCC TTGCCTCACA ACTTCAGCTC CATTAATTCG CCGTCAGCTC 
TCACATGACC ACGAATCTGT TGGCCCTCCT AGCCTGGATG CTCAGCCCAA CTCAAAGACA 
GAAAGATCAA AATCATATGA TGAGGGTCTG GATGATTACA GAGAAGATGC AAAATTGTCC 
TTTAAGCACG TATCTAGTCT GAAGGGAATC AAGATCGCAG ACAGCCAAAA GTCATCAGAA 
GACTCTGGGT CCAGAAAAGA TTCTTCCTCA GAGGTCTTCA GTGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCCGACCCCT TGTCACCGAT AAGGGCAAGC GAGTTGGTGG AAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGTCATTCAC TTTACCTGTA CAAAGATAAA 
AGAGAGCAGA CGACTCCGTC TGAGGAAGAG CAGCCCATCA GTGTTAATGC TTGCTTGATA 
GACATCTCTT ACAGTGAGAC CAAGAGGAAA AATGTGTTTC GACTCACCAC GTCCGACTGT 
GAATGCCTGT TTCAGGCTGA AGACAGAGAT GATATGCTAG CTTGGATCAA GACGATCCAG 
GAGAGCAGCA ACCTAAACGA AGAGGACACT GGAGTCACTA ACAGGGATCT AATTAGTCGA 
AGAATAAAAG AATACAACAA TCTGATGAGC AAAGCAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTCA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACACTCTC CGAAGGAAGA GTCGGAAAGG AAACTTCTCA GTAAAGATGA TACCAGTCCC 
CCAAAAGACA AAGGCACATG GAGAAAAGGC ATTCCAAGTA TCATGAGAAA GACATTTGAG 
AAAAAGCCAA CTGCTACAGG AACTTTCGGC GTCCGACTAG ATGACTGCCC ACCAGCTCAT 
ACTAATCGGT ATATTCCATT AATAGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGAGGT 
CTTGAATATA CAGGTATTTA TAGAGTTCCT GGAAATAATG CAGCCATCTC AAGTATGCAA 
GAAGAACTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAAC TCCCTGAGCC TCTCTTCACA 
AATGATAAAT ATGCTGATTT TATTGAAGCC AATCGTAAAG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCACGATTTG CCTGAACATC ATTATGAAAC ACTTAAGTTC 
CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCAA TAGTGTTTGG TCCCACCCTT GTTCGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACCC ACATGCCTGA CCAGTACAAG ATTGTAGAAA CGCTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG CCTCTTACAA CAGTGCAGGA GGAAAGCACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGA 
GTCTCCCCAG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCTG GAAAGGATCA GTATAGCAGG GAACTGCTTG TGTCCTCCAT CTTTGCAGCT 
GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA AAAGCACAGC CTAGCAGCTC AGAAGATGAA 
CTGGACAATG TATTTTTTAA GAAAGAAAAT GTGGAACAGT GTCACAATGA TACTAAAGAG 
GAGTCCAAAA AAGAAAGTGA GACACTGGGC AGAAAACAGA AGATCATCAT TGCCAAAGAA 
AACAGCACTA GGAAAGACCC CAGCACGACA AAAGATGAAA AGATATCACT AGGAAAAGAG 
AGCACGCCTT CTGAAGAACC CTCACCACCA CACAACTCAA AACACAACAA GTCACCAACT 
CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG AGCCCCAGGT CACTTCTGGC ACAGAAGTCC 
TCCCACCTTG AAGAGACAGG CTCTGACTCT GGCACTTTGC TCAGCACGTC TTCCCAGGCC 
TCCCTGGCAA GGTTTTCCAT GAAGAAATCA ACCAGTCCAG AAACGAAACA TAGCGAGTTT 
TTGGCCAACG TCAGCACCAT CACCTCAGAT TATTCCACCA CATCGTCTGC TACATACTTG 
ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG 
GACGAGGCAG ATGACGAGAG AAGCGAACTC ATCAGTGAAG GGCGGCCTGT GGAAACCGAC 
AGCGAGAGCG AGTTTCCCGT GTTCCCCACA GCCTTGACTT CAGAGAGGCT TTTCCGAGGA 
AAACTGCAAG AAGTGACTAA GAGCAGCCGG AGAAATTCTG AAGGAAGTGA ATTAAGTTGC 
ACCGAGGGAA GTTTAACATC AAGTTTAGAT AGCCGGAGAC AGCTCTTCAG TTCCCATAAA 
CTCATCGAAT GTGATACTCT TTCCAGGAAA AAATCAGCTA GATTCAAGTC AGATAGTGGA 
AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA GCACCTTCGT TAACTAAAGT GTTTGATGTT 
ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA CTGACACCCA CCAGAGGCGA ATCCGAAAAA 
CAGGAACCCA CATGGAAAAC GAAAATAGCA GATCGGTTAA AACTGAGACC CAGAGCCCCT 
GCGGATGACA TGTTTGGAGT AGGGAATCAC AAAGTGAATG CCGAGACTGC TAAAAGGAAA 
AGCATCCGGC GCAGACATAC ACTAGGAGGG CACAGAGATG CTACCGAAAT CAGCGTTTTG 
AATTTTTGGA AAGTGCATGA GCAGAGCGGG GAGAGAGAAT CTGAACTTTC AGCTGTAAAC 
CGGTTAAAAC CAAAATGCTC AGCCCAGGAC CTTTCCATCT CAGACTGGCT GGCCAGGGAA 
CGCCTACGCA CCAGTACCTC TGACCTTAGC AGAGGAGAAA TCGGAGATCC CCAGACAGAG 
AACCCAAGCA CACGAGAAAT AGCCACGACC GACACACCTT TGTCTCTTCA TTGCAACACA 
GGCAGTTCTT CCAGCACCTT GGCTTCAACA AACAGGCCCC TTCTTTCCAT ACCACCACAG 
TCACCTGACC AAATAAACGG AGAAAGCTTC CAGAACGTGA GCAAAAATGC TAGTTCTGCA 
GCGAATGCCC AACCTCATAA ACTGTCTGAA ACCCCAGGCA GTAAAGCAGA GTTTCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517907.1
CDS	2241..8189
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_011519605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519605.2

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGGCATA TTCTCAAGAT GCCTACCTGA AAGGCAACGA AGCTTATAGC 
GGCAATGCCC GCAATATACC TGAACCTCCA CCAATCTGCT ATCCCTGGCT GCCATCTGCC 
CCATCAGCCA TGGCACAGCC AGTTGAAATA TCTCCTCCTG ACTCATCATT GAGCAAACAG 
CAAACCAGTA CACCAGTACT GACACAACCT GGTAGGGCCT ATAGAATGGA AATACAAGTG 
CCTCCATCAC CAACAGATGT TGCAAAATCA AACACAGCAG TGTGTGTTTG CAATGAAAGT 
GTAAGGACTG TCATTGTGCC TTCTGAGAAG GTTGTAGATT TGTTATCCAA TAGAAACAAC 
CATACAGGTC CTTCACATAG AACTGAAGAA GTGAGGTATG GCGTGAGTGA GCAGACCTCT 
TTAAAAACAG TGTCAAGAAC CACATCACCA CCATTATCAA TTCCCACCAC TCATCTAATT 
CATCAGCCTG CAGGCTCCAG ATCACTGGAA CCTTCTGGAA TTTTACTTAA GTCTGGAAAT 
TACAGTGGAC ATTCTGATGG AATCTCAAGC AGCAGATCTC AAGCTGTGGA GGCTCCCTCT 
GTATCTGTTA ATCACTATTC GCCAAATTCC CATCAGCACA TAGACTGGAA AAACTATAAA 
ACTTACAAAG AGTATATTGA TAACAGACGA TTGCACATAG GTTGTCGGAC AATACAAGAA 
AGATTAGATA GTTTAAGAGC AGCATCTCAA AGCACGACAG ATTATAACCA GGTCGTCCCC 
AACCGCACTA CTTTGCAGGG ACGACGTCGA AGCACCTCTC ATGATCGAGT GCCCCAGTCT 
GTCCAGATAC GGCAACGCAG TGTGTCCCAA GAAAGACTGG AAGATTCTGT GCTAATGAAG 
TATTGTCCAA GAAGTGCATC TCAAGGAGCA CTGACGTCTC CATCTGTTAG TTTTAGTAAT 
CATAGAACTC GTTCATGGGA TTATATTGAG GGACAGGATG AAACCTTAGA AAATGTCAAT 
TCTGGAACTC CAATACCTGA TTCCAATGGA GAGAAAAAAC AGACTTACAA GTGGAGTGGG 
TTTACTGAAC AGGATGATAG ACGAGGTATT TGTGAAAGAC CTAGGCAGCA AGAAATTCAT 
AAATCTTTTC GAGGTTCCAA TTTTACTGTG GCTCCAAGCG TTGTTAATTC TGATAACAGG 
CGAATGAGTG GTAGAGGAGT GGGATCTGTG TCGCAGTTTA AAAAAATTCC ACCAGATCTA 
AAAACATTGC AGTCAAACAG AAATTTTCAG ACTACTTGTG GAATGTCACT GCCTCGGGGT 
ATTTCACAAG ACAGGTCACC TCTTGTGAAA GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC 
ACGCATGTCA CAAAACCATC ATTTAGCCAG AAATCATTTG TTTCTATCAA AGACCAAAGA 
CCAGTAAATC ACTTGCATCA GAACAGTCTG TTGAATCAGC AGACATGGGT AAGGACTGAC 
AGTGCCCCCG ATCAGCAAGT GGAGACTGGG AAATCCCCCT CTTTATCTGG AGCCTCTGCC 
AAGCCTGCCC CTCAGTCGAG TGAAAACGCT GGTACTTCAG ATTTAGAACT ACCTGTCAGT 
CAAAGGAATC AAGATTTAAG TTTACAAGAG GCTGAAACTG AGCAATCAGA TACTTTAGAT 
AATAAAGAAG CTGTCATCCT AAGGGAAAAA CCTCCATCTG GACGCCAGAC ACCGCAGCCT 
TTAAGGCATC AGTCTTACAT CTTGGCAGTA AATGACCAGG AGACCGGGTC AGACACTACC 
TGCTGGCTGC CCAATGATGC ACGTCGAGAG GTCCACATAA AAAGAATGGA GGAAAGAAAA 
GCCTCGAGTA CCAGTCCGCC TGGCGATTCT TTGGCTTCCA TCCCATTTAT AGTAAAAGGA 
ATTTATTATA AAGAATGCAA CAATACTGAA ATGAGTAAGC CCAATGTTAG TCTCTTCTCC 
CACGCCTTCT CCATTCAGTC CTCCTTTAGA ACAGATGAAC CAACTAGCCC TAGCATTGAT 
CATGATATTG CACATATCCC TGCCTCTGCT GTTATATCAG CCTCTACCTC TCAGGTCCCC 
TCCATAGCAA CAGTTCCTCC TTGCCTCACA ACTTCAGCTC CATTAATTCG CCGTCAGCTC 
TCACATGACC ACGAATCTGT TGGCCCTCCT AGCCTGGATG CTCAGCCCAA CTCAAAGACA 
GAAAGATCAA AATCATATGA TGAGGGTCTG GATGATTACA GAGAAGATGC AAAATTGTCC 
TTTAAGCACG TATCTAGTCT GAAGGGAATC AAGATCGCAG ACAGCCAAAA GTCATCAGAA 
GACTCTGGGT CCAGAAAAGA TTCTTCCTCA GAGGTCTTCA GTGATGCTGC CAAGGAAGGG 
TGGCTTCATT TCCGACCCCT TGTCACCGAT AAGGGCAAGC GAGTTGGTGG AAGTATTCGG 
CCATGGAAAC AGATGTATGT TGTCCTTCGG GGTCATTCAC TTTACCTGTA CAAAGATAAA 
AGAGAGCAGA CGACTCCGTC TGAGGAAGAG CAGCCCATCA GTGTTAATGC TTGCTTGATA 
GACATCTCTT ACAGTGAGAC CAAGAGGAAA AATGTGTTTC GACTCACCAC GTCCGACTGT 
GAATGCCTGT TTCAGGCTGA AGACAGAGAT GATATGCTAG CTTGGATCAA GACGATCCAG 
GAGAGCAGCA ACCTAAACGA AGAGGACACT GGAGTCACTA ACAGGGATCT AATTAGTCGA 
AGAATAAAAG AATACAACAA TCTGATGAGC AAAGCAGAAC AGTTGCCAAA AACACCTCGC 
CAGAGTCTCA GCATCAGGCA AACTTTGCTT GGTGCTAAAT CAGAGCCAAA GACTCAAAGC 
CCACACTCTC CGAAGGAAGA GTCGGAAAGG AAACTTCTCA GTAAAGATGA TACCAGTCCC 
CCAAAAGACA AAGGCACATG GAGAAAAGGC ATTCCAAGTA TCATGAGAAA GACATTTGAG 
AAAAAGCCAA CTGCTACAGG AACTTTCGGC GTCCGACTAG ATGACTGCCC ACCAGCTCAT 
ACTAATCGGT ATATTCCATT AATAGTTGAC ATATGTTGCA AATTAGTTGA AGAAAGAGGT 
CTTGAATATA CAGGTATTTA TAGAGTTCCT GGAAATAATG CAGCCATCTC AAGTATGCAA 
GAAGAACTCA ACAAGGGAAT GGCTGATATT GATATACAAG ATGATAAATG GCGAGATTTG 
AATGTGATAA GCAGTTTACT AAAATCCTTC TTCAGAAAAC TCCCTGAGCC TCTCTTCACA 
AATGATAAAT ATGCTGATTT TATTGAAGCC AATCGTAAAG AAGATCCTCT AGATCGTCTG 
AAAACATTAA AAAGACTAAT TCACGATTTG CCTGAACATC ATTATGAAAC ACTTAAGTTC 
CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA AATTCAGAAA AAAATAAGAT GGAACCAAGA 
AACCTAGCAA TAGTGTTTGG TCCCACCCTT GTTCGAACAT CAGAAGACAA CATGACCCAC 
ATGGTCACCC ACATGCCTGA CCAGTACAAG ATTGTAGAAA CGCTCATCCA GCACCATGAC 
TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG CCTCTTACAA CAGTGCAGGA GGAAAGCACA 
GTAGACTCCC AGCCAGTGCC AAACATAGAT CATTTACTCA CCAACATTGG AAGGACAGGA 
GTCTCCCCAG GAGATGTATC AGATTCAGCT ACTAGTGACT CAACAAAATC TAAGGGTTCT 
TGGGGATCTG GAAAGGATCA GTATAGCAGG GAACTGCTTG TGTCCTCCAT CTTTGCAGCT 
GCTAGTCGCA AGAGGAAGAA GCCGAAAGAA AAAGCACAGC CTAGCAGCTC AGAAGATGAA 
CTGGACAATG TATTTTTTAA GAAAGAAAAT GTGGAACAGT GTCACAATGA TACTAAAGAG 
GAGTCCAAAA AAGAAAGTGA GACACTGGGC AGAAAACAGA AGATCATCAT TGCCAAAGAA 
AACAGCACTA GGAAAGACCC CAGCACGACA AAAGATGAAA AGATATCACT AGGAAAAGAG 
AGCACGCCTT CTGAAGAACC CTCACCACCA CACAACTCAA AACACAACAA GTCACCAACT 
CTCAGCTGTC GCTTTGCCAT CCTGAAAGAG AGCCCCAGGT CACTTCTGGC ACAGAAGTCC 
TCCCACCTTG AAGAGACAGG CTCTGACTCT GGCACTTTGC TCAGCACGTC TTCCCAGGCC 
TCCCTGGCAA GGTTTTCCAT GAAGAAATCA ACCAGTCCAG AAACGAAACA TAGCGAGTTT 
TTGGCCAACG TCAGCACCAT CACCTCAGAT TATTCCACCA CATCGTCTGC TACATACTTG 
ACTAGCCTGG ACTCCAGTCG ACTGAGCCCT GAGGTGCAAT CCGTGGCAGA GAGCAAGGGG 
GACGAGGCAG ATGACGAGAG AAGCGAACTC ATCAGTGAAG GGCGGCCTGT GGAAACCGAC 
AGCGAGAGCG AGTTTCCCGT GTTCCCCACA GCCTTGACTT CAGAGAGGCT TTTCCGAGGA 
AAACTGCAAG AAGTGACTAA GAGCAGCCGG AGAAATTCTG AAGGAAGTGA ATTAAGTTGC 
ACCGAGGGAA GTTTAACATC AAGTTTAGAT AGCCGGAGAC AGCTCTTCAG TTCCCATAAA 
CTCATCGAAT GTGATACTCT TTCCAGGAAA AAATCAGCTA GATTCAAGTC AGATAGTGGA 
AGTCTAGGAG ATGCCAAGAA TGAGAAAGAA GCACCTTCGT TAACTAAAGT GTTTGATGTT 
ATGAAAAAAG GAAAGTCAAC TGGGAGTTTA CTGACACCCA CCAGAGGCGA ATCCGAAAAA 
CAGGAACCCA CATGGAAAAC GAAAATAGCA GATCGGTTAA AACTGAGACC CAGAGCCCCT 
GCGGATGACA TGTTTGGAGT AGGGAATCAC AAAGTGAATG CCGAGACTGC TAAAAGGAAA 
AGCATCCGGC GCAGACATAC ACTAGGAGGG CACAGAGATG CTACCGAAAT CAGCGTTTTG 
AATTTTTGGA AAGTGCATGA GCAGAGCGGG GAGAGAGAAT CTGAACTTTC AGCTGTAAAC 
CGGTTAAAAC CAAAATGCTC AGCCCAGGAC CTTTCCATCT CAGACTGGCT GGCCAGGGAA 
CGCCTACGCA CCAGTACCTC TGACCTTAGC AGAGGAGAAA TCGGAGATCC CCAGACAGAG 
AACCCAAGCA CACGAGAAAT AGCCACGACC GACACACCTT TGTCTCTTCA TTGCAACACA 
GGCAGTTCTT CCAGCACCTT GGCTTCAACA AACAGGCCCC TTCTTTCCAT ACCACCACAG 
TCACCTGACC AAATAAACGG AGAAAGCTTC CAGAACGTGA GCAAAAATGC TAGTTCTGCA 
GCGAATGCCC AACCTCATAA ACTGTCTGAA ACCCCAGGCA GTAAAGCAGA GTTTCATCCC 
TGTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519606.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517908.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X10, mRNA.

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CloneID	OHu70671
Clone ID Related Accession (Same CDS sequence)	XM_011519606.2
Accession Version	XM_011519606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTC TGTGCAGCTG GTCAGTCTCT CAGCCATTAC TTTGGAAACA GGAAACGCCT 
CAAGGAAAAC AAAGAAACCG CTTGGAACCA ATGGATACCA TATTTGTTAA GCAAGTTAAA 
GAAGGAGGAC CTGCTTTTGA AGCTGGATTA TGTACAGGTG ACCGAATTAT AAAAGTCAAT 
GGAGAAAGTG TTATTGGCAA AACCTATTCC CAAGTAATTG CTTTAATTCA AAACAGTGAT 
ACAACATTGG AACTTAGTGT TATGCCAAAA GATGAAGACA TTCTCCAAGT GCTACAGTTT 
ACAAAGGATG TCACAGCACT GGCATATTCT CAAGATGCCT ACCTGAAAGG CAACGAAGCT 
TATAGCGGCA ATGCCCGCAA TATACCTGAA CCTCCACCAA TCTGCTATCC CTGGCTGCCA 
TCTGCCCCAT CAGCCATGGC ACAGCCAGTT GAAATATCTC CTCCTGACTC ATCATTGAGC 
AAACAGCAAA CCAGTACACC AGTACTGACA CAACCTGGTA GGGCCTATAG AATGGAAATA 
CAAGTGCCTC CATCACCAAC AGATGTTGCA AAATCAAACA CAGCAGTGTG TGTTTGCAAT 
GAAAGTGTAA GGACTGTCAT TGTGCCTTCT GAGAAGGTTG TAGATTTGTT ATCCAATAGA 
AACAACCATA CAGGTCCTTC ACATAGAACT GAAGAAGTGA GGTATGGCGT GAGTGAGCAG 
ACCTCTTTAA AAACAGTGTC AAGAACCACA TCACCACCAT TATCAATTCC CACCACTCAT 
CTAATTCATC AGCCTGCAGG CTCCAGATCA CTGGAACCTT CTGGAATTTT ACTTAAGTCT 
GGAAATTACA GTGGACATTC TGATGGAATC TCAAGCAGCA GATCTCAAGC TGTGGAGGCT 
CCCTCTGTAT CTGTTAATCA CTATTCGCCA AATTCCCATC AGCACATAGA CTGGAAAAAC 
TATAAAACTT ACAAAGAGTA TATTGATAAC AGACGATTGC ACATAGGTTG TCGGACAATA 
CAAGAAAGAT TAGATAGTTT AAGAGCAGCA TCTCAAAGCA CGACAGATTA TAACCAGGTC 
GTCCCCAACC GCACTACTTT GCAGGGACGA CGTCGAAGCA CCTCTCATGA TCGAGTGCCC 
CAGTCTGTCC AGATACGGCA ACGCAGTGTG TCCCAAGAAA GACTGGAAGA TTCTGTGCTA 
ATGAAGTATT GTCCAAGAAG TGCATCTCAA GGAGCACTGA CGTCTCCATC TGTTAGTTTT 
AGTAATCATA GAACTCGTTC ATGGGATTAT ATTGAGGGAC AGGATGAAAC CTTAGAAAAT 
GTCAATTCTG GAACTCCAAT ACCTGATTCC AATGGAGAGA AAAAACAGAC TTACAAGTGG 
AGTGGGTTTA CTGAACAGGA TGATAGACGA GGTATTTGTG AAAGACCTAG GCAGCAAGAA 
ATTCATAAAT CTTTTCGAGG TTCCAATTTT ACTGTGGCTC CAAGCGTTGT TAATTCTGAT 
AACAGGCGAA TGAGTGGTAG AGGAGTGGGA TCTGTGTCGC AGTTTAAAAA AATTCCACCA 
GATCTAAAAA CATTGCAGTC AAACAGAAAT TTTCAGACTA CTTGTGGAAT GTCACTGCCT 
CGGGGTATTT CACAAGACAG GTCACCTCTT GTGAAAGTCC GAAGTAATTC TCTGAAAGCT 
CCTTCCACGC ATGTCACAAA ACCATCATTT AGCCAGAAAT CATTTGTTTC TATCAAAGAC 
CAAAGACCAG TAAATCACTT GCATCAGAAC AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG 
ACTGACAGTG CCCCCGATCA GCAAGTGGAG ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC 
TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA AACGCTGGTA CTTCAGATTT AGAACTACCT 
GTCAGTCAAA GGAATCAAGA TTTAAGTTTA CAAGAGGCTG AAACTGAGCA ATCAGATACT 
TTAGATAATA AAGAAGCTGT CATCCTAAGG GAAAAACCTC CATCTGGACG CCAGACACCG 
CAGCCTTTAA GGCATCAGTC TTACATCTTG GCAGTAAATG ACCAGGAGAC CGGGTCAGAC 
ACTACCTGCT GGCTGCCCAA TGATGCACGT CGAGAGGTCC ACATAAAAAG AATGGAGGAA 
AGAAAAGCCT CGAGTACCAG TCCGCCTGGC GATTCTTTGG CTTCCATCCC ATTTATAGTA 
AAAGGAATTT ATTATAAAGA ATGCAACAAT ACTGAAATGA GTAAGCCCAA TGTTAGTCTC 
TTCTCCCACG CCTTCTCCAT TCAGTCCTCC TTTAGAACAG ATGAACCAAC TAGCCCTAGC 
ATTGATCATG ATATTGCACA TATCCCTGCC TCTGCTGTTA TATCAGCCTC TACCTCTCAG 
GTCCCCTCCA TAGCAACAGT TCCTCCTTGC CTCACAACTT CAGCTCCATT AATTCGCCGT 
CAGCTCTCAC ATGACCACGA ATCTGTTGGC CCTCCTAGCC TGGATGCTCA GCCCAACTCA 
AAGACAGAAA GATCAAAATC ATATGATGAG GGTCTGGATG ATTACAGAGA AGATGCAAAA 
TTGTCCTTTA AGCACGTATC TAGTCTGAAG GGAATCAAGA TCGCAGACAG CCAAAAGTCA 
TCAGAAGACT CTGGGTCCAG AAAAGATTCT TCCTCAGAGG TCTTCAGTGA TGCTGCCAAG 
GAAGGGTGGC TTCATTTCCG ACCCCTTGTC ACCGATAAGG GCAAGCGAGT TGGTGGAAGT 
ATTCGGCCAT GGAAACAGAT GTATGTTGTC CTTCGGGGTC ATTCACTTTA CCTGTACAAA 
GATAAAAGAG AGCAGACGAC TCCGTCTGAG GAAGAGCAGC CCATCAGTGT TAATGCTTGC 
TTGATAGACA TCTCTTACAG TGAGACCAAG AGGAAAAATG TGTTTCGACT CACCACGTCC 
GACTGTGAAT GCCTGTTTCA GGCTGAAGAC AGAGATGATA TGCTAGCTTG GATCAAGACG 
ATCCAGGAGA GCAGCAACCT AAACGAAGAG GACACTGGAG TCACTAACAG GGATCTAATT 
AGTCGAAGAA TAAAAGAATA CAACAATCTG ATGAGCAAAG CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTCAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ACTCTCCGAA GGAAGAGTCG GAAAGGAAAC TTCTCAGTAA AGATGATACC 
AGTCCCCCAA AAGACAAAGG CACATGGAGA AAAGGCATTC CAAGTATCAT GAGAAAGACA 
TTTGAGAAAA AGCCAACTGC TACAGGAACT TTCGGCGTCC GACTAGATGA CTGCCCACCA 
GCTCATACTA ATCGGTATAT TCCATTAATA GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGAGGTCTTG AATATACAGG TATTTATAGA GTTCCTGGAA ATAATGCAGC CATCTCAAGT 
ATGCAAGAAG AACTCAACAA GGGAATGGCT GATATTGATA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAACTCCC TGAGCCTCTC 
TTCACAAATG ATAAATATGC TGATTTTATT GAAGCCAATC GTAAAGAAGA TCCTCTAGAT 
CGTCTGAAAA CATTAAAAAG ACTAATTCAC GATTTGCCTG AACATCATTA TGAAACACTT 
AAGTTCCTTT CAGCTCATCT GAAGACAGTG GCAGAAAATT CAGAAAAAAA TAAGATGGAA 
CCAAGAAACC TAGCAATAGT GTTTGGTCCC ACCCTTGTTC GAACATCAGA AGACAACATG 
ACCCACATGG TCACCCACAT GCCTGACCAG TACAAGATTG TAGAAACGCT CATCCAGCAC 
CATGACTGGT TTTTCACAGA AGAAGGTGCT GAAGAGCCTC TTACAACAGT GCAGGAGGAA 
AGCACAGTAG ACTCCCAGCC AGTGCCAAAC ATAGATCATT TACTCACCAA CATTGGAAGG 
ACAGGAGTCT CCCCAGGAGA TGTATCAGAT TCAGCTACTA GTGACTCAAC AAAATCTAAG 
GGTTCTTGGG GATCTGGAAA GGATCAGTAT AGCAGGGAAC TGCTTGTGTC CTCCATCTTT 
GCAGCTGCTA GTCGCAAGAG GAAGAAGCCG AAAGAAAAAG CACAGCCTAG CAGCTCAGAA 
GATGAACTGG ACAATGTATT TTTTAAGAAA GAAAATGTGG AACAGTGTCA CAATGATACT 
AAAGAGGAGT CCAAAAAAGA AAGTGAGACA CTGGGCAGAA AACAGAAGAT CATCATTGCC 
AAAGAAAACA GCACTAGGAA AGACCCCAGC ACGACAAAAG ATGAAAAGAT ATCACTAGGA 
AAAGAGAGCA CGCCTTCTGA AGAACCCTCA CCACCACACA ACTCAAAACA CAACAAGTCA 
CCAACTCTCA GCTGTCGCTT TGCCATCCTG AAAGAGAGCC CCAGGTCACT TCTGGCACAG 
AAGTCCTCCC ACCTTGAAGA GACAGGCTCT GACTCTGGCA CTTTGCTCAG CACGTCTTCC 
CAGGCCTCCC TGGCAAGGTT TTCCATGAAG AAATCAACCA GTCCAGAAAC GAAACATAGC 
GAGTTTTTGG CCAACGTCAG CACCATCACC TCAGATTATT CCACCACATC GTCTGCTACA 
TACTTGACTA GCCTGGACTC CAGTCGACTG AGCCCTGAGG TGCAATCCGT GGCAGAGAGC 
AAGGGGGACG AGGCAGATGA CGAGAGAAGC GAACTCATCA GTGAAGGGCG GCCTGTGGAA 
ACCGACAGCG AGAGCGAGTT TCCCGTGTTC CCCACAGCCT TGACTTCAGA GAGGCTTTTC 
CGAGGAAAAC TGCAAGAAGT GACTAAGAGC AGCCGGAGAA ATTCTGAAGG AAGTGAATTA 
AGTTGCACCG AGGGAAGTTT AACATCAAGT TTAGATAGCC GGAGACAGCT CTTCAGTTCC 
CATAAACTCA TCGAATGTGA TACTCTTTCC AGGAAAAAAT CAGCTAGATT CAAGTCAGAT 
AGTGGAAGTC TAGGAGATGC CAAGAATGAG AAAGAAGCAC CTTCGTTAAC TAAAGTGTTT 
GATGTTATGA AAAAAGGAAA GTCAACTGGG AGTTTACTGA CACCCACCAG AGGCGAATCC 
GAAAAACAGG AACCCACATG GAAAACGAAA ATAGCAGATC GGTTAAAACT GAGACCCAGA 
GCCCCTGCGG ATGACATGTT TGGAGTAGGG AATCACAAAG TGAATGCCGA GACTGCTAAA 
AGGAAAAGCA TCCGGCGCAG ACATACACTA GGAGGGCACA GAGATGCTAC CGAAATCAGC 
GTTTTGAATT TTTGGAAAGT GCATGAGCAG AGCGGGGAGA GAGAATCTGA ACTTTCAGCT 
GTAAACCGGT TAAAACCAAA ATGCTCAGCC CAGGACCTTT CCATCTCAGA CTGGCTGGCC 
AGGGAACGCC TACGCACCAG TACCTCTGAC CTTAGCAGAG GAGAAATCGG AGATCCCCAG 
ACAGAGAACC CAAGCACACG AGAAATAGCC ACGACCGACA CACCTTTGTC TCTTCATTGC 
AACACAGGCA GTTCTTCCAG CACCTTGGCT TCAACAAACA GGCCCCTTCT TTCCATACCA 
CCACAGTCAC CTGACCAAAT AAACGGAGAA AGCTTCCAGA ACGTGAGCAA AAATGCTAGT 
TCTGCAGCGA ATGCCCAACC TCATAAACTG TCTGAAACCC CAGGCAGTAA AGCAGAGTTT 
CATCCCTGTC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517908.1
CDS	2310..8084
Translation	MEALCSWSVSQPLLWKQETPQGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQKIIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLEETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL

Target ORF information:

RefSeq Version	XM_011519606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519606.2

ORF Insert Sequence:

ATGGAAGCTC TGTGCAGCTG GTCAGTCTCT CAGCCATTAC TTTGGAAACA GGAAACGCCT 
CAAGGAAAAC AAAGAAACCG CTTGGAACCA ATGGATACCA TATTTGTTAA GCAAGTTAAA 
GAAGGAGGAC CTGCTTTTGA AGCTGGATTA TGTACAGGTG ACCGAATTAT AAAAGTCAAT 
GGAGAAAGTG TTATTGGCAA AACCTATTCC CAAGTAATTG CTTTAATTCA AAACAGTGAT 
ACAACATTGG AACTTAGTGT TATGCCAAAA GATGAAGACA TTCTCCAAGT GCTACAGTTT 
ACAAAGGATG TCACAGCACT GGCATATTCT CAAGATGCCT ACCTGAAAGG CAACGAAGCT 
TATAGCGGCA ATGCCCGCAA TATACCTGAA CCTCCACCAA TCTGCTATCC CTGGCTGCCA 
TCTGCCCCAT CAGCCATGGC ACAGCCAGTT GAAATATCTC CTCCTGACTC ATCATTGAGC 
AAACAGCAAA CCAGTACACC AGTACTGACA CAACCTGGTA GGGCCTATAG AATGGAAATA 
CAAGTGCCTC CATCACCAAC AGATGTTGCA AAATCAAACA CAGCAGTGTG TGTTTGCAAT 
GAAAGTGTAA GGACTGTCAT TGTGCCTTCT GAGAAGGTTG TAGATTTGTT ATCCAATAGA 
AACAACCATA CAGGTCCTTC ACATAGAACT GAAGAAGTGA GGTATGGCGT GAGTGAGCAG 
ACCTCTTTAA AAACAGTGTC AAGAACCACA TCACCACCAT TATCAATTCC CACCACTCAT 
CTAATTCATC AGCCTGCAGG CTCCAGATCA CTGGAACCTT CTGGAATTTT ACTTAAGTCT 
GGAAATTACA GTGGACATTC TGATGGAATC TCAAGCAGCA GATCTCAAGC TGTGGAGGCT 
CCCTCTGTAT CTGTTAATCA CTATTCGCCA AATTCCCATC AGCACATAGA CTGGAAAAAC 
TATAAAACTT ACAAAGAGTA TATTGATAAC AGACGATTGC ACATAGGTTG TCGGACAATA 
CAAGAAAGAT TAGATAGTTT AAGAGCAGCA TCTCAAAGCA CGACAGATTA TAACCAGGTC 
GTCCCCAACC GCACTACTTT GCAGGGACGA CGTCGAAGCA CCTCTCATGA TCGAGTGCCC 
CAGTCTGTCC AGATACGGCA ACGCAGTGTG TCCCAAGAAA GACTGGAAGA TTCTGTGCTA 
ATGAAGTATT GTCCAAGAAG TGCATCTCAA GGAGCACTGA CGTCTCCATC TGTTAGTTTT 
AGTAATCATA GAACTCGTTC ATGGGATTAT ATTGAGGGAC AGGATGAAAC CTTAGAAAAT 
GTCAATTCTG GAACTCCAAT ACCTGATTCC AATGGAGAGA AAAAACAGAC TTACAAGTGG 
AGTGGGTTTA CTGAACAGGA TGATAGACGA GGTATTTGTG AAAGACCTAG GCAGCAAGAA 
ATTCATAAAT CTTTTCGAGG TTCCAATTTT ACTGTGGCTC CAAGCGTTGT TAATTCTGAT 
AACAGGCGAA TGAGTGGTAG AGGAGTGGGA TCTGTGTCGC AGTTTAAAAA AATTCCACCA 
GATCTAAAAA CATTGCAGTC AAACAGAAAT TTTCAGACTA CTTGTGGAAT GTCACTGCCT 
CGGGGTATTT CACAAGACAG GTCACCTCTT GTGAAAGTCC GAAGTAATTC TCTGAAAGCT 
CCTTCCACGC ATGTCACAAA ACCATCATTT AGCCAGAAAT CATTTGTTTC TATCAAAGAC 
CAAAGACCAG TAAATCACTT GCATCAGAAC AGTCTGTTGA ATCAGCAGAC ATGGGTAAGG 
ACTGACAGTG CCCCCGATCA GCAAGTGGAG ACTGGGAAAT CCCCCTCTTT ATCTGGAGCC 
TCTGCCAAGC CTGCCCCTCA GTCGAGTGAA AACGCTGGTA CTTCAGATTT AGAACTACCT 
GTCAGTCAAA GGAATCAAGA TTTAAGTTTA CAAGAGGCTG AAACTGAGCA ATCAGATACT 
TTAGATAATA AAGAAGCTGT CATCCTAAGG GAAAAACCTC CATCTGGACG CCAGACACCG 
CAGCCTTTAA GGCATCAGTC TTACATCTTG GCAGTAAATG ACCAGGAGAC CGGGTCAGAC 
ACTACCTGCT GGCTGCCCAA TGATGCACGT CGAGAGGTCC ACATAAAAAG AATGGAGGAA 
AGAAAAGCCT CGAGTACCAG TCCGCCTGGC GATTCTTTGG CTTCCATCCC ATTTATAGTA 
AAAGGAATTT ATTATAAAGA ATGCAACAAT ACTGAAATGA GTAAGCCCAA TGTTAGTCTC 
TTCTCCCACG CCTTCTCCAT TCAGTCCTCC TTTAGAACAG ATGAACCAAC TAGCCCTAGC 
ATTGATCATG ATATTGCACA TATCCCTGCC TCTGCTGTTA TATCAGCCTC TACCTCTCAG 
GTCCCCTCCA TAGCAACAGT TCCTCCTTGC CTCACAACTT CAGCTCCATT AATTCGCCGT 
CAGCTCTCAC ATGACCACGA ATCTGTTGGC CCTCCTAGCC TGGATGCTCA GCCCAACTCA 
AAGACAGAAA GATCAAAATC ATATGATGAG GGTCTGGATG ATTACAGAGA AGATGCAAAA 
TTGTCCTTTA AGCACGTATC TAGTCTGAAG GGAATCAAGA TCGCAGACAG CCAAAAGTCA 
TCAGAAGACT CTGGGTCCAG AAAAGATTCT TCCTCAGAGG TCTTCAGTGA TGCTGCCAAG 
GAAGGGTGGC TTCATTTCCG ACCCCTTGTC ACCGATAAGG GCAAGCGAGT TGGTGGAAGT 
ATTCGGCCAT GGAAACAGAT GTATGTTGTC CTTCGGGGTC ATTCACTTTA CCTGTACAAA 
GATAAAAGAG AGCAGACGAC TCCGTCTGAG GAAGAGCAGC CCATCAGTGT TAATGCTTGC 
TTGATAGACA TCTCTTACAG TGAGACCAAG AGGAAAAATG TGTTTCGACT CACCACGTCC 
GACTGTGAAT GCCTGTTTCA GGCTGAAGAC AGAGATGATA TGCTAGCTTG GATCAAGACG 
ATCCAGGAGA GCAGCAACCT AAACGAAGAG GACACTGGAG TCACTAACAG GGATCTAATT 
AGTCGAAGAA TAAAAGAATA CAACAATCTG ATGAGCAAAG CAGAACAGTT GCCAAAAACA 
CCTCGCCAGA GTCTCAGCAT CAGGCAAACT TTGCTTGGTG CTAAATCAGA GCCAAAGACT 
CAAAGCCCAC ACTCTCCGAA GGAAGAGTCG GAAAGGAAAC TTCTCAGTAA AGATGATACC 
AGTCCCCCAA AAGACAAAGG CACATGGAGA AAAGGCATTC CAAGTATCAT GAGAAAGACA 
TTTGAGAAAA AGCCAACTGC TACAGGAACT TTCGGCGTCC GACTAGATGA CTGCCCACCA 
GCTCATACTA ATCGGTATAT TCCATTAATA GTTGACATAT GTTGCAAATT AGTTGAAGAA 
AGAGGTCTTG AATATACAGG TATTTATAGA GTTCCTGGAA ATAATGCAGC CATCTCAAGT 
ATGCAAGAAG AACTCAACAA GGGAATGGCT GATATTGATA TACAAGATGA TAAATGGCGA 
GATTTGAATG TGATAAGCAG TTTACTAAAA TCCTTCTTCA GAAAACTCCC TGAGCCTCTC 
TTCACAAATG ATAAATATGC TGATTTTATT GAAGCCAATC GTAAAGAAGA TCCTCTAGAT 
CGTCTGAAAA CATTAAAAAG ACTAATTCAC GATTTGCCTG AACATCATTA TGAAACACTT 
AAGTTCCTTT CAGCTCATCT GAAGACAGTG GCAGAAAATT CAGAAAAAAA TAAGATGGAA 
CCAAGAAACC TAGCAATAGT GTTTGGTCCC ACCCTTGTTC GAACATCAGA AGACAACATG 
ACCCACATGG TCACCCACAT GCCTGACCAG TACAAGATTG TAGAAACGCT CATCCAGCAC 
CATGACTGGT TTTTCACAGA AGAAGGTGCT GAAGAGCCTC TTACAACAGT GCAGGAGGAA 
AGCACAGTAG ACTCCCAGCC AGTGCCAAAC ATAGATCATT TACTCACCAA CATTGGAAGG 
ACAGGAGTCT CCCCAGGAGA TGTATCAGAT TCAGCTACTA GTGACTCAAC AAAATCTAAG 
GGTTCTTGGG GATCTGGAAA GGATCAGTAT AGCAGGGAAC TGCTTGTGTC CTCCATCTTT 
GCAGCTGCTA GTCGCAAGAG GAAGAAGCCG AAAGAAAAAG CACAGCCTAG CAGCTCAGAA 
GATGAACTGG ACAATGTATT TTTTAAGAAA GAAAATGTGG AACAGTGTCA CAATGATACT 
AAAGAGGAGT CCAAAAAAGA AAGTGAGACA CTGGGCAGAA AACAGAAGAT CATCATTGCC 
AAAGAAAACA GCACTAGGAA AGACCCCAGC ACGACAAAAG ATGAAAAGAT ATCACTAGGA 
AAAGAGAGCA CGCCTTCTGA AGAACCCTCA CCACCACACA ACTCAAAACA CAACAAGTCA 
CCAACTCTCA GCTGTCGCTT TGCCATCCTG AAAGAGAGCC CCAGGTCACT TCTGGCACAG 
AAGTCCTCCC ACCTTGAAGA GACAGGCTCT GACTCTGGCA CTTTGCTCAG CACGTCTTCC 
CAGGCCTCCC TGGCAAGGTT TTCCATGAAG AAATCAACCA GTCCAGAAAC GAAACATAGC 
GAGTTTTTGG CCAACGTCAG CACCATCACC TCAGATTATT CCACCACATC GTCTGCTACA 
TACTTGACTA GCCTGGACTC CAGTCGACTG AGCCCTGAGG TGCAATCCGT GGCAGAGAGC 
AAGGGGGACG AGGCAGATGA CGAGAGAAGC GAACTCATCA GTGAAGGGCG GCCTGTGGAA 
ACCGACAGCG AGAGCGAGTT TCCCGTGTTC CCCACAGCCT TGACTTCAGA GAGGCTTTTC 
CGAGGAAAAC TGCAAGAAGT GACTAAGAGC AGCCGGAGAA ATTCTGAAGG AAGTGAATTA 
AGTTGCACCG AGGGAAGTTT AACATCAAGT TTAGATAGCC GGAGACAGCT CTTCAGTTCC 
CATAAACTCA TCGAATGTGA TACTCTTTCC AGGAAAAAAT CAGCTAGATT CAAGTCAGAT 
AGTGGAAGTC TAGGAGATGC CAAGAATGAG AAAGAAGCAC CTTCGTTAAC TAAAGTGTTT 
GATGTTATGA AAAAAGGAAA GTCAACTGGG AGTTTACTGA CACCCACCAG AGGCGAATCC 
GAAAAACAGG AACCCACATG GAAAACGAAA ATAGCAGATC GGTTAAAACT GAGACCCAGA 
GCCCCTGCGG ATGACATGTT TGGAGTAGGG AATCACAAAG TGAATGCCGA GACTGCTAAA 
AGGAAAAGCA TCCGGCGCAG ACATACACTA GGAGGGCACA GAGATGCTAC CGAAATCAGC 
GTTTTGAATT TTTGGAAAGT GCATGAGCAG AGCGGGGAGA GAGAATCTGA ACTTTCAGCT 
GTAAACCGGT TAAAACCAAA ATGCTCAGCC CAGGACCTTT CCATCTCAGA CTGGCTGGCC 
AGGGAACGCC TACGCACCAG TACCTCTGAC CTTAGCAGAG GAGAAATCGG AGATCCCCAG 
ACAGAGAACC CAAGCACACG AGAAATAGCC ACGACCGACA CACCTTTGTC TCTTCATTGC 
AACACAGGCA GTTCTTCCAG CACCTTGGCT TCAACAAACA GGCCCCTTCT TTCCATACCA 
CCACAGTCAC CTGACCAAAT AAACGGAGAA AGCTTCCAGA ACGTGAGCAA AAATGCTAGT 
TCTGCAGCGA ATGCCCAACC TCATAAACTG TCTGAAACCC CAGGCAGTAA AGCAGAGTTT 
CATCCCTGTC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGAP21 ( XM_011519607.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517909.1 Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X12, mRNA.

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CloneID	OHu70672
Clone ID Related Accession (Same CDS sequence)	XM_011519607.2
Accession Version	XM_011519607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho GTPase-activating protein 21 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTATTC AGCTACTAGT GACTCAACAA AATCTAAGGG TTCTTGGGGA TCTGGAAAGG 
ATCAGTATAG CAGGGAACTG CTTGTGTCCT CCATCTTTGC AGCTGCTAGT CGCAAGAGGA 
AGAAGCCGAA AGAAAAAGCA CAGCCTAGCA GCTCAGAAGA TGAACTGGAC AATGTATTTT 
TTAAGAAAGA AAATGTGGAA CAGTGTCACA ATGATACTAA AGAGGAGTCC AAAAAAGAAA 
GTGAGACACT GGGCAGAAAA CAGAAGATCA TCATTGCCAA AGAAAACAGC ACTAGGAAAG 
ACCCCAGCAC GACAAAAGAT GAAAAGATAT CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517909.1
CDS	2241..6716
Translation	MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSAPSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGNYSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSTTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSNHRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTVAPSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPSTHVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENAGTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIVKGIYYKECNNTEMSKPNVSLFSHAFSIQSSFRTDEPTSPSIDHDIAHIPASAVISASTSQVPSIATVPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQTTPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLKRLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDWFFTEEGAEEPLIQLLVTQQNLRVLGDLERISIAGNCLCPPSLQLLVARGRSRKKKHSLAAQKMNWTMYFLRKKMWNSVTMILKRSPKKKVRHWAENRRSSLPKKTALGKTPARQKMKRYH

Target ORF information:

RefSeq Version	XM_011519607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho GTPase activating protein 21 (ARHGAP21), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519607.2

ORF Insert Sequence:

ATGATGGCCA CGCGTCGGAC TGGTCTGTCT GAGGGAGATG GTGACAAGCT CAAGGCCTGC 
GAGGTCTCAA AAAATAAAGA TGGAAAAGAA CAAAGTGAAA CTGTATCACT GTCTGAAGAT 
GAAACATTCT CCTGGCCAGG TCCCAAAACA GTTACGTTGA AAAGAACATC TCAAGGCTTT 
GGTTTTACAT TAAGACATTT TATTGTTTAT CCCCCAGAGT CTGCAATTCA ATTTTCATAT 
AAGGATGAAG AAAATGGAAA CAGAGGAGGA AAACAAAGAA ACCGCTTGGA ACCAATGGAT 
ACCATATTTG TTAAGCAAGT TAAAGAAGGA GGACCTGCTT TTGAAGCTGG ATTATGTACA 
GGTGACCGAA TTATAAAAGT CAATGGAGAA AGTGTTATTG GCAAAACCTA TTCCCAAGTA 
ATTGCTTTAA TTCAAAACAG TGATACAACA TTGGAACTTA GTGTTATGCC AAAAGATGAA 
GACATTCTCC AAGTGCTACA GTTTACAAAG GATGTCACAG CACTGGCATA TTCTCAAGAT 
GCCTACCTGA AAGGCAACGA AGCTTATAGC GGCAATGCCC GCAATATACC TGAACCTCCA 
CCAATCTGCT ATCCCTGGCT GCCATCTGCC CCATCAGCCA TGGCACAGCC AGTTGAAATA 
TCTCCTCCTG ACTCATCATT GAGCAAACAG CAAACCAGTA CACCAGTACT GACACAACCT 
GGTAGGGCCT ATAGAATGGA AATACAAGTG CCTCCATCAC CAACAGATGT TGCAAAATCA 
AACACAGCAG TGTGTGTTTG CAATGAAAGT GTAAGGACTG TCATTGTGCC TTCTGAGAAG 
GTTGTAGATT TGTTATCCAA TAGAAACAAC CATACAGGTC CTTCACATAG AACTGAAGAA 
GTGAGGTATG GCGTGAGTGA GCAGACCTCT TTAAAAACAG TGTCAAGAAC CACATCACCA 
CCATTATCAA TTCCCACCAC TCATCTAATT CATCAGCCTG CAGGCTCCAG ATCACTGGAA 
CCTTCTGGAA TTTTACTTAA GTCTGGAAAT TACAGTGGAC ATTCTGATGG AATCTCAAGC 
AGCAGATCTC AAGCTGTGGA GGCTCCCTCT GTATCTGTTA ATCACTATTC GCCAAATTCC 
CATCAGCACA TAGACTGGAA AAACTATAAA ACTTACAAAG AGTATATTGA TAACAGACGA 
TTGCACATAG GTTGTCGGAC AATACAAGAA AGATTAGATA GTTTAAGAGC AGCATCTCAA 
AGCACGACAG ATTATAACCA GGTCGTCCCC AACCGCACTA CTTTGCAGGG ACGACGTCGA 
AGCACCTCTC ATGATCGAGT GCCCCAGTCT GTCCAGATAC GGCAACGCAG TGTGTCCCAA 
GAAAGACTGG AAGATTCTGT GCTAATGAAG TATTGTCCAA GAAGTGCATC TCAAGGAGCA 
CTGACGTCTC CATCTGTTAG TTTTAGTAAT CATAGAACTC GTTCATGGGA TTATATTGAG 
GGACAGGATG AAACCTTAGA AAATGTCAAT TCTGGAACTC CAATACCTGA TTCCAATGGA 
GAGAAAAAAC AGACTTACAA GTGGAGTGGG TTTACTGAAC AGGATGATAG ACGAGGTATT 
TGTGAAAGAC CTAGGCAGCA AGAAATTCAT AAATCTTTTC GAGGTTCCAA TTTTACTGTG 
GCTCCAAGCG TTGTTAATTC TGATAACAGG CGAATGAGTG GTAGAGGAGT GGGATCTGTG 
TCGCAGTTTA AAAAAATTCC ACCAGATCTA AAAACATTGC AGTCAAACAG AAATTTTCAG 
ACTACTTGTG GAATGTCACT GCCTCGGGGT ATTTCACAAG ACAGGTCACC TCTTGTGAAA 
GTCCGAAGTA ATTCTCTGAA AGCTCCTTCC ACGCATGTCA CAAAACCATC ATTTAGCCAG 
AAATCATTTG TTTCTATCAA AGACCAAAGA CCAGTAAATC ACTTGCATCA GAACAGTCTG 
TTGAATCAGC AGACATGGGT AAGGACTGAC AGTGCCCCCG ATCAGCAAGT GGAGACTGGG 
AAATCCCCCT CTTTATCTGG AGCCTCTGCC AAGCCTGCCC CTCAGTCGAG TGAAAACGCT 
GGTACTTCAG ATTTAGAACT ACCTGTCAGT CAAAGGAATC AAGATTTAAG TTTACAAGAG 
GCTGAAACTG AGCAATCAGA TACTTTAGAT AATAAAGAAG CTGTCATCCT AAGGGAAAAA 
CCTCCATCTG GACGCCAGAC ACCGCAGCCT TTAAGGCATC AGTCTTACAT CTTGGCAGTA 
AATGACCAGG AGACCGGGTC AGACACTACC TGCTGGCTGC CCAATGATGC ACGTCGAGAG 
GTCCACATAA AAAGAATGGA GGAAAGAAAA GCCTCGAGTA CCAGTCCGCC TGGCGATTCT 
TTGGCTTCCA TCCCATTTAT AGTAAAAGGA ATTTATTATA AAGAATGCAA CAATACTGAA 
ATGAGTAAGC CCAATGTTAG TCTCTTCTCC CACGCCTTCT CCATTCAGTC CTCCTTTAGA 
ACAGATGAAC CAACTAGCCC TAGCATTGAT CATGATATTG CACATATCCC TGCCTCTGCT 
GTTATATCAG CCTCTACCTC TCAGGTCCCC TCCATAGCAA CAGTTCCTCC TTGCCTCACA 
ACTTCAGCTC CATTAATTCG CCGTCAGCTC TCACATGACC ACGAATCTGT TGGCCCTCCT 
AGCCTGGATG CTCAGCCCAA CTCAAAGACA GAAAGATCAA AATCATATGA TGAGGGTCTG 
GATGATTACA GAGAAGATGC AAAATTGTCC TTTAAGCACG TATCTAGTCT GAAGGGAATC 
AAGATCGCAG ACAGCCAAAA GTCATCAGAA GACTCTGGGT CCAGAAAAGA TTCTTCCTCA 
GAGGTCTTCA GTGATGCTGC CAAGGAAGGG TGGCTTCATT TCCGACCCCT TGTCACCGAT 
AAGGGCAAGC GAGTTGGTGG AAGTATTCGG CCATGGAAAC AGATGTATGT TGTCCTTCGG 
GGTCATTCAC TTTACCTGTA CAAAGATAAA AGAGAGCAGA CGACTCCGTC TGAGGAAGAG 
CAGCCCATCA GTGTTAATGC TTGCTTGATA GACATCTCTT ACAGTGAGAC CAAGAGGAAA 
AATGTGTTTC GACTCACCAC GTCCGACTGT GAATGCCTGT TTCAGGCTGA AGACAGAGAT 
GATATGCTAG CTTGGATCAA GACGATCCAG GAGAGCAGCA ACCTAAACGA AGAGGACACT 
GGAGTCACTA ACAGGGATCT AATTAGTCGA AGAATAAAAG AATACAACAA TCTGATGAGC 
AAAGCAGAAC AGTTGCCAAA AACACCTCGC CAGAGTCTCA GCATCAGGCA AACTTTGCTT 
GGTGCTAAAT CAGAGCCAAA GACTCAAAGC CCACACTCTC CGAAGGAAGA GTCGGAAAGG 
AAACTTCTCA GTAAAGATGA TACCAGTCCC CCAAAAGACA AAGGCACATG GAGAAAAGGC 
ATTCCAAGTA TCATGAGAAA GACATTTGAG AAAAAGCCAA CTGCTACAGG AACTTTCGGC 
GTCCGACTAG ATGACTGCCC ACCAGCTCAT ACTAATCGGT ATATTCCATT AATAGTTGAC 
ATATGTTGCA AATTAGTTGA AGAAAGAGGT CTTGAATATA CAGGTATTTA TAGAGTTCCT 
GGAAATAATG CAGCCATCTC AAGTATGCAA GAAGAACTCA ACAAGGGAAT GGCTGATATT 
GATATACAAG ATGATAAATG GCGAGATTTG AATGTGATAA GCAGTTTACT AAAATCCTTC 
TTCAGAAAAC TCCCTGAGCC TCTCTTCACA AATGATAAAT ATGCTGATTT TATTGAAGCC 
AATCGTAAAG AAGATCCTCT AGATCGTCTG AAAACATTAA AAAGACTAAT TCACGATTTG 
CCTGAACATC ATTATGAAAC ACTTAAGTTC CTTTCAGCTC ATCTGAAGAC AGTGGCAGAA 
AATTCAGAAA AAAATAAGAT GGAACCAAGA AACCTAGCAA TAGTGTTTGG TCCCACCCTT 
GTTCGAACAT CAGAAGACAA CATGACCCAC ATGGTCACCC ACATGCCTGA CCAGTACAAG 
ATTGTAGAAA CGCTCATCCA GCACCATGAC TGGTTTTTCA CAGAAGAAGG TGCTGAAGAG 
CCTCTTATTC AGCTACTAGT GACTCAACAA AATCTAAGGG TTCTTGGGGA TCTGGAAAGG 
ATCAGTATAG CAGGGAACTG CTTGTGTCCT CCATCTTTGC AGCTGCTAGT CGCAAGAGGA 
AGAAGCCGAA AGAAAAAGCA CAGCCTAGCA GCTCAGAAGA TGAACTGGAC AATGTATTTT 
TTAAGAAAGA AAATGTGGAA CAGTGTCACA ATGATACTAA AGAGGAGTCC AAAAAAGAAA 
GTGAGACACT GGGCAGAAAA CAGAAGATCA TCATTGCCAA AGAAAACAGC ACTAGGAAAG 
ACCCCAGCAC GACAAAAGAT GAAAAGATAT CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hematopoietic defects in response to reduced Arhgap21.
Stem cell research2617-27(2017 Nov)
Xavier-Ferrucio J,Ricon L,Vieira K,Longhini AL,Lazarini M,Bigarella CL,Franchi G Jr,Krause DS,Saad STO

PTEN controls glandular morphogenesis through a juxtamembrane ?-Arrestin1/ARHGAP21 scaffolding complex.
eLife6(2017 Jul)
Javadi A,Deevi RK,Evergren E,Blondel-Tepaz E,Baillie GS,Scott MG,Campbell FC

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Genetic association of ARHGAP21 gene variant with mandibular prognathism.
Journal of dental research94(4)569-76(2015 Apr)
Perillo L,Monsurr? A,Bonci E,Torella A,Mutarelli M,Nigro V

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ARHGAP21 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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