WDR35 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WDR35
Entrez Gene ID	57539
Full Name	WD repeat domain 35
Synonyms	CED2,IFT121,IFTA1,SRTD7
General protein information	Preferred Names WD repeat domain 35 Names WD repeat-containing protein 35 intraflagellar transport protein 121 homolog naofen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p24.1
Summary	This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Two patients with Sensenbrenner syndrome / cranioectodermal dysplasia (CED) were identified with mutations in this gene, consistent with a possible ciliary function.[provided by RefSeq, Sep 2010].
Disorder MIM:	613602
Disorder Html:	Cranioectodermal dysplasia 2, 613610 (3); Short-rib thoracic dysplasia 7 with or without polydactyly, 614091 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_020779.3	NP_065830.2	WD repeat-containing protein 35 isoform 2
XM_011533007.2	XP_011531309.1	WD repeat-containing protein 35 isoform X1
NM_001006657.1	NP_001006658.1	WD repeat-containing protein 35 isoform 1
NM_001006657.2	NP_001006658.1	WD repeat-containing protein 35 isoform 1
NM_020779.4	NP_065830.2	WD repeat-containing protein 35 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WDR35	XP_515315.3
Bos taurus (cattle)	WDR35	XP_002691565.2
Xenopus tropicalis (tropical clawed frog)	wdr35	XP_002934527.2
Mus musculus (house mouse)	Wdr35	NP_766058.3
Danio rerio (zebrafish)	wdr35	NP_001139061.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011562	XP_309945.3
Homo sapiens (human)	WDR35	NP_001006658.1
Canis lupus familiaris (dog)	WDR35	XP_005630221.1
Gallus gallus (chicken)	WDR35	XP_419970.3
Caenorhabditis elegans (roundworm)	ifta-1	NP_508940.3
	WDR35	XP_001107482.2
Rattus norvegicus (Norway rat)	Wdr35l	NP_001092810.1
Drosophila melanogaster (fruit fly)	Oseg4	NP_001261278.1

Gene Ontology
Bibliography

Related articles in PubMed

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.
Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, Nakayama K
Human molecular genetics(2017 Dec)

Uncommon runs of homozygosity disclose homozygous missense mutations in two ciliopathy-related genes (SPAG17 and WDR35) in a patient with multiple brain and skeletal anomalies.
C?rdova-Fletes C, Becerra-Solano LE, Rangel-Sosa MM, Rivas-Estilla AM, Alberto Gal?n-Huerta K, Ortiz-L?pez R, Rojas-Mart?nez A, Ju?rez-V?zquez CI, Garc?a-Ortiz JE
European journal of medical genetics(2017 Nov)

Clinical and molecular genetic characterization of a male patient with Sensenbrenner syndrome (cranioectodermal dysplasia) and biallelic WDR35 mutations.
Walczak-Sztulpa J, Wawrocka A, Swiader-Lesniak A, Socha M, Jamsheer A, Drozdz D, Latos-Bielenska A, Zachwieja K
Birth defects research(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Psychomotor development was apparently normal. Molecular analysis in one of the affected individuals identified compound heterozygosity for a nonsense (c.1922T>G, p.(Leu641*)) and missense (c.2522A>T, p.(Asp841Val)) variants in WDR35. We
Title: Intrafamilial phenotypic variability in a Polish family with Sensenbrenner syndrome and biallelic WDR35 mutations.

Wdr35 regulates cilium assembly by selectively regulating transport of distinct cargoes.
Title: Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

Splicing variants in WDR35, and possibly in other IFT-A components, underlie a number of Ellis-van Creveld syndrome cases by disrupting targeting of both the EvC complex and Smoothened to cilia.
Title: Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium.

report on the detection of novel WDR35 mutations in two unrelated cranioectodermal dysplasia patients
Title: Novel WDR35 mutations in patients with cranioectodermal dysplasia (Sensenbrenner syndrome).

A pathogenic WDR35 mutation was identified in a family with a complex clinical presentation that includes significant overlap of the phenotypes described in Sensenbrenner syndrome and the unclassified short-rib polydactyly syndromes.
Title: WDR35 mutation in siblings with Sensenbrenner syndrome: a ciliopathy with variable phenotype.

The following WDR35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WDR35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu70585	XM_011533007.2 Latest version!	Homo sapiens WD repeat domain 35 (WDR35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu18950	NM_020779.4 Latest version!	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu19673	NM_001006657.2 Latest version!	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$832.30 $1189.00
View Old Accession Versions >>
OHu19673	NM_001006657.1	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$832.30 $1189.00
OHu18950	NM_020779.3	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WDR35 ( XM_011533007.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531309.1 Homo sapiens WD repeat domain 35 (WDR35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu70585
Clone ID Related Accession (Same CDS sequence)	XM_011533007.2
Accession Version	XM_011533007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	WD repeat-containing protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAAA CCCATGTGAT AGCAGCCTCG AAAGAAGCAT TTTATACCTG GCAATATCGT 
GTGGCAAAGA AGCTCACAGC ATTGGAAATT AATCAGATCA CACGGTCTCG AAAAGAAGGG 
AGAGAAAGAA TTTATCATGT TGATGATACC CCTTCTGGAT CAATGGATGG TGTGCTTGAT 
TATAGTAAAA CCATTCAAGG CACAAGGGAT CCAATTTGTG CCATAACTGC ATCAGATAAG 
ATATTGATTG TGGGTCGTGA ATCTGGCACC ATTCAGAGAT ACAGTCTACC TAATGTTGGT 
TTGATTCAAA AATATTCCCT TAATTGTCGA GCCTACCAGT TATCCTTGAA TTGCAACTCT 
AGCCGTCTTG CTATCATAGA CATCTCAGGA GTTCTGACTT TCTTTGACTT GGATGCTCGA 
GTAACGGACA GTACGGGACA GCAAGTAGTT GGAGAGTTGT TAAAATTGGA ACGAAGAGAT 
GTCTGGGATA TGAAGTGGGC CAAAGATAAT CCTGATTTGT TTGCAATGAT GGAGAAGACA 
AGAATGTATG TTTTCAGAAA CTTGGATCCT GAGGAACCCA TTCAGACCTC TGGATATATT 
TGTAATTTTG AGGATTTAGA AATTAAATCT GTTCTTTTGG ATGAGATATT AAAGGATCCA 
GAACATCCAA ACAAGGATTA CCTAATTAAC TTTGAGATTC GGTCTCTGCG AGATAGCCGA 
GCACTGATTG AGAAGGTTGG AATTAAAGAT GCATCTCAGT TCATAGAGGA CAATCCACAC 
CCCCGACTTT GGCGCCTACT GGCTGAAGCA GCTCTTCAGA AACTGGATCT ATACACTGCA 
GAGCAAGCAT TTGTGCGCTG CAAAGATTAC CAAGGCATTA AGTTTGTGAA GCGCTTGGGC 
AAACTACTGA GTGAGTCAAT GAAACAGGCT GAAGTTGTTG GCTACTTCGG CAGGTTTGAA 
GAGGCTGAAA GAACGTATCT CGAGATGGAC AGAAGGGATC TTGCTATTGG CCTCCGGCTG 
AAATTGGGGG ATTGGTTTAG AGTACTCCAG CTCCTGAAAA CTGGATCTGG TGATGCAGAT 
GACAGTCTCC TGGAACAAGC CAACAATGCC ATTGGAGACT ACTTTGCTGA TCGACAAAAG 
TGGTTGAATG CTGTACAATA TTATGTACAA GGACGGAACC AGGAACGCTT AGCTGAATGT 
TACTATATGT TAGAGGATTA TGAAGGGTTA GAGAACCTTG CCATTTCACT TCCAGAAAAC 
CACAAGTTAC TTCCAGAAAT AGCACAAATG TTTGTCAGAG TTGGAATGTG TGAACAAGCA 
GTGACTGCAT TTTTGAAATG TAGTCAACCA AAGGCAGCAG TAGATACCTG CGTACATCTC 
AACCAATGGA ACAAAGCTGT TGAATTGGCT AAAAATCATA GTATGAAAGA AATTGGATCT 
CTGTTAGCTA GGTATGCATC TCATTTACTG GAAAAGAATA AAACTCTTGA TGCCATAGAA 
CTCTATCGGA AAGCCAATTA CTTTTTTGAT GCAGCTAAAC TGATGTTTAA GATTGCAGAT 
GAAGAGGCAA AGAAAGGAAG TAAACCTTTA CGTGTCAAGA AGCTCTATGT ACTGTCAGCC 
TTACTTATAG AGCAATACCA TGAACAGATG AAGAATGCCC AGCGAGGAAA AGTTAAAGGA 
AAAAGTTCAG AGGCCACTTC TGCCTTGGCT GGTTTGCTGG AAGAAGAAGT TCTGTCTACA 
ACAGATCGTT TCACAGATAA TGCATGGAGA GGGGCAGAGG CTTACCACTT CTTTATACTT 
GCACAGAGGC AGCTCTATGA GGGATGTGTG GACACTGCAC TGAAGACAGC TCTTCACCTG 
AAAGACTATG AAGACATCAT CCCTCCTGTG GAGATCTACT CTCTGCTAGC ACTCTGCGCA 
TGCGCCAGCA GAGCCTTTGG GACTTGTTCA AAAGCTTTCA TTAAACTTAA ATCTTTAGAG 
ACCCTCAGTT CAGAACAGAA ACAGCAGTAT GAAGACCTTG CTTTAGAAAT CTTCACCAAA 
CATACTTCAA AAGATAACAG AAAACCTGAA TTGGACAGCC TTATGGAAGG TGGAGAAGGG 
AAACTGCCAA CATGCGTTGC CACAGGAAGC CCAATCACTG AGTATCAATT CTGGATGTGC 
AGTGTATGCA AACACGGTGT CCTTGCTCAG GAAATAAGCC ACTACAGCTT CTGCCCCTTA 
TGCCATAGTC CAGTGGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531309.1
CDS	106..2346
Translation	MTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDTPSGSMDGVLDYSKTIQGTRDPICAITASDKILIVGRESGTIQRYSLPNVGLIQKYSLNCRAYQLSLNCNSSRLAIIDISGVLTFFDLDARVTDSTGQQVVGELLKLERRDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPNKDYLINFEIRSLRDSRALIEKVGIKDASQFIEDNPHPRLWRLLAEAALQKLDLYTAEQAFVRCKDYQGIKFVKRLGKLLSESMKQAEVVGYFGRFEEAERTYLEMDRRDLAIGLRLKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGDYFADRQKWLNAVQYYVQGRNQERLAECYYMLEDYEGLENLAISLPENHKLLPEIAQMFVRVGMCEQAVTAFLKCSQPKAAVDTCVHLNQWNKAVELAKNHSMKEIGSLLARYASHLLEKNKTLDAIELYRKANYFFDAAKLMFKIADEEAKKGSKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKSSEATSALAGLLEEEVLSTTDRFTDNAWRGAEAYHFFILAQRQLYEGCVDTALKTALHLKDYEDIIPPVEIYSLLALCACASRAFGTCSKAFIKLKSLETLSSEQKQQYEDLALEIFTKHTSKDNRKPELDSLMEGGEGKLPTCVATGSPITEYQFWMCSVCKHGVLAQEISHYSFCPLCHSPVG

Target ORF information:

RefSeq Version	XM_011533007.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain 35 (WDR35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533007.2

ORF Insert Sequence:

ATGACCAAAA CCCATGTGAT AGCAGCCTCG AAAGAAGCAT TTTATACCTG GCAATATCGT 
GTGGCAAAGA AGCTCACAGC ATTGGAAATT AATCAGATCA CACGGTCTCG AAAAGAAGGG 
AGAGAAAGAA TTTATCATGT TGATGATACC CCTTCTGGAT CAATGGATGG TGTGCTTGAT 
TATAGTAAAA CCATTCAAGG CACAAGGGAT CCAATTTGTG CCATAACTGC ATCAGATAAG 
ATATTGATTG TGGGTCGTGA ATCTGGCACC ATTCAGAGAT ACAGTCTACC TAATGTTGGT 
TTGATTCAAA AATATTCCCT TAATTGTCGA GCCTACCAGT TATCCTTGAA TTGCAACTCT 
AGCCGTCTTG CTATCATAGA CATCTCAGGA GTTCTGACTT TCTTTGACTT GGATGCTCGA 
GTAACGGACA GTACGGGACA GCAAGTAGTT GGAGAGTTGT TAAAATTGGA ACGAAGAGAT 
GTCTGGGATA TGAAGTGGGC CAAAGATAAT CCTGATTTGT TTGCAATGAT GGAGAAGACA 
AGAATGTATG TTTTCAGAAA CTTGGATCCT GAGGAACCCA TTCAGACCTC TGGATATATT 
TGTAATTTTG AGGATTTAGA AATTAAATCT GTTCTTTTGG ATGAGATATT AAAGGATCCA 
GAACATCCAA ACAAGGATTA CCTAATTAAC TTTGAGATTC GGTCTCTGCG AGATAGCCGA 
GCACTGATTG AGAAGGTTGG AATTAAAGAT GCATCTCAGT TCATAGAGGA CAATCCACAC 
CCCCGACTTT GGCGCCTACT GGCTGAAGCA GCTCTTCAGA AACTGGATCT ATACACTGCA 
GAGCAAGCAT TTGTGCGCTG CAAAGATTAC CAAGGCATTA AGTTTGTGAA GCGCTTGGGC 
AAACTACTGA GTGAGTCAAT GAAACAGGCT GAAGTTGTTG GCTACTTCGG CAGGTTTGAA 
GAGGCTGAAA GAACGTATCT CGAGATGGAC AGAAGGGATC TTGCTATTGG CCTCCGGCTG 
AAATTGGGGG ATTGGTTTAG AGTACTCCAG CTCCTGAAAA CTGGATCTGG TGATGCAGAT 
GACAGTCTCC TGGAACAAGC CAACAATGCC ATTGGAGACT ACTTTGCTGA TCGACAAAAG 
TGGTTGAATG CTGTACAATA TTATGTACAA GGACGGAACC AGGAACGCTT AGCTGAATGT 
TACTATATGT TAGAGGATTA TGAAGGGTTA GAGAACCTTG CCATTTCACT TCCAGAAAAC 
CACAAGTTAC TTCCAGAAAT AGCACAAATG TTTGTCAGAG TTGGAATGTG TGAACAAGCA 
GTGACTGCAT TTTTGAAATG TAGTCAACCA AAGGCAGCAG TAGATACCTG CGTACATCTC 
AACCAATGGA ACAAAGCTGT TGAATTGGCT AAAAATCATA GTATGAAAGA AATTGGATCT 
CTGTTAGCTA GGTATGCATC TCATTTACTG GAAAAGAATA AAACTCTTGA TGCCATAGAA 
CTCTATCGGA AAGCCAATTA CTTTTTTGAT GCAGCTAAAC TGATGTTTAA GATTGCAGAT 
GAAGAGGCAA AGAAAGGAAG TAAACCTTTA CGTGTCAAGA AGCTCTATGT ACTGTCAGCC 
TTACTTATAG AGCAATACCA TGAACAGATG AAGAATGCCC AGCGAGGAAA AGTTAAAGGA 
AAAAGTTCAG AGGCCACTTC TGCCTTGGCT GGTTTGCTGG AAGAAGAAGT TCTGTCTACA 
ACAGATCGTT TCACAGATAA TGCATGGAGA GGGGCAGAGG CTTACCACTT CTTTATACTT 
GCACAGAGGC AGCTCTATGA GGGATGTGTG GACACTGCAC TGAAGACAGC TCTTCACCTG 
AAAGACTATG AAGACATCAT CCCTCCTGTG GAGATCTACT CTCTGCTAGC ACTCTGCGCA 
TGCGCCAGCA GAGCCTTTGG GACTTGTTCA AAAGCTTTCA TTAAACTTAA ATCTTTAGAG 
ACCCTCAGTT CAGAACAGAA ACAGCAGTAT GAAGACCTTG CTTTAGAAAT CTTCACCAAA 
CATACTTCAA AAGATAACAG AAAACCTGAA TTGGACAGCC TTATGGAAGG TGGAGAAGGG 
AAACTGCCAA CATGCGTTGC CACAGGAAGC CCAATCACTG AGTATCAATT CTGGATGTGC 
AGTGTATGCA AACACGGTGT CCTTGCTCAG GAAATAAGCC ACTACAGCTT CTGCCCCTTA 
TGCCATAGTC CAGTGGGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WDR35 ( NM_020779.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065830.2 Homo sapiens WD repeat domain 35 (WDR35), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18950
Clone ID Related Accession (Same CDS sequence)	NM_020779.3 , NM_020779.4
Accession Version	NM_020779.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	WD repeat-containing protein 35 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB037757.2, BC036659.2, BX476609.1 and BC033951.1. On Dec 3, 2004 this sequence version replaced NM_020779.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform 2 thus lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.221908.1, SRR1660805.171847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGTTTGTA 
CTAGTTCTTT GTAATTCTAT TGGTACACCC TTGGATCCCA AATACATTGA TATTGTACCA 
TTGTTTGTTG CAATGACCAA AACCCATGTG ATAGCAGCCT CGAAAGAAGC ATTTTATACC 
TGGCAATATC GTGTGGCAAA GAAGCTCACA GCATTGGAAA TTAATCAGAT CACACGGTCT 
CGAAAAGAAG GGAGAGAAAG AATTTATCAT GTTGATGATA CCCCTTCTGG ATCAATGGAT 
GGTGTGCTTG ATTATAGTAA AACCATTCAA GGCACAAGGG ATCCAATTTG TGCCATAACT 
GCATCAGATA AGATATTGAT TGTGGGTCGT GAATCTGGCA CCATTCAGAG ATACAGTCTA 
CCTAATGTTG GTTTGATTCA AAAATATTCC CTTAATTGTC GAGCCTACCA GTTATCCTTG 
AATTGCAACT CTAGCCGTCT TGCTATCATA GACATCTCAG GAGTTCTGAC TTTCTTTGAC 
TTGGATGCTC GAGTAACGGA CAGTACGGGA CAGCAAGTAG TTGGAGAGTT GTTAAAATTG 
GAACGAAGAG ATGTCTGGGA TATGAAGTGG GCCAAAGATA ATCCTGATTT GTTTGCAATG 
ATGGAGAAGA CAAGAATGTA TGTTTTCAGA AACTTGGATC CTGAGGAACC CATTCAGACC 
TCTGGATATA TTTGTAATTT TGAGGATTTA GAAATTAAAT CTGTTCTTTT GGATGAGATA 
TTAAAGGATC CAGAACATCC AAACAAGGAT TACCTAATTA ACTTTGAGAT TCGGTCTCTG 
CGAGATAGCC GAGCACTGAT TGAGAAGGTT GGAATTAAAG ATGCATCTCA GTTCATAGAG 
GACAATCCAC ACCCCCGACT TTGGCGCCTA CTGGCTGAAG CAGCTCTTCA GAAACTGGAT 
CTATACACTG CAGAGCAAGC ATTTGTGCGC TGCAAAGATT ACCAAGGCAT TAAGTTTGTG 
AAGCGCTTGG GCAAACTACT GAGTGAGTCA ATGAAACAGG CTGAAGTTGT TGGCTACTTC 
GGCAGGTTTG AAGAGGCTGA AAGAACGTAT CTCGAGATGG ACAGAAGGGA TCTTGCTATT 
GGCCTCCGGC TGAAATTGGG GGATTGGTTT AGAGTACTCC AGCTCCTGAA AACTGGATCT 
GGTGATGCAG ATGACAGTCT CCTGGAACAA GCCAACAATG CCATTGGAGA CTACTTTGCT 
GATCGACAAA AGTGGTTGAA TGCTGTACAA TATTATGTAC AAGGACGGAA CCAGGAACGC 
TTAGCTGAAT GTTACTATAT GTTAGAGGAT TATGAAGGGT TAGAGAACCT TGCCATTTCA 
CTTCCAGAAA ACCACAAGTT ACTTCCAGAA ATAGCACAAA TGTTTGTCAG AGTTGGAATG 
TGTGAACAAG CAGTGACTGC ATTTTTGAAA TGTAGTCAAC CAAAGGCAGC AGTAGATACC 
TGCGTACATC TCAACCAATG GAACAAAGCT GTTGAATTGG CTAAAAATCA TAGTATGAAA 
GAAATTGGAT CTCTGTTAGC TAGGTATGCA TCTCATTTAC TGGAAAAGAA TAAAACTCTT 
GATGCCATAG AACTCTATCG GAAAGCCAAT TACTTTTTTG ATGCAGCTAA ACTGATGTTT 
AAGATTGCAG ATGAAGAGGC AAAGAAAGGA AGTAAACCTT TACGTGTCAA GAAGCTCTAT 
GTACTGTCAG CCTTACTTAT AGAGCAATAC CATGAACAGA TGAAGAATGC CCAGCGAGGA 
AAAGTTAAAG GAAAAAGTTC AGAGGCCACT TCTGCCTTGG CTGGTTTGCT GGAAGAAGAA 
GTTCTGTCTA CAACAGATCG TTTCACAGAT AATGCATGGA GAGGGGCAGA GGCTTACCAC 
TTCTTTATAC TTGCACAGAG GCAGCTCTAT GAGGGATGTG TGGACACTGC ACTGAAGACA 
GCTCTTCACC TGAAAGACTA TGAAGACATC ATCCCTCCTG TGGAGATCTA CTCTCTGCTA 
GCACTCTGCG CATGCGCCAG CAGAGCCTTT GGGACTTGTT CAAAAGCTTT CATTAAACTT 
AAATCTTTAG AGACCCTCAG TTCAGAACAG AAACAGCAGT ATGAAGACCT TGCTTTAGAA 
ATCTTCACCA AACATACTTC AAAAGATAAC AGAAAACCTG AATTGGACAG CCTTATGGAA 
GGTGGAGAAG GGAAACTGCC AACATGCGTT GCCACAGGAA GCCCAATCAC TGAGTATCAA 
TTCTGGATGT GCAGTGTATG CAAACACGGT GTCCTTGCTC AGGAAATAAG CCACTACAGC 
TTCTGCCCCT TATGCCATAG TCCAGTGGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065830.2
CDS	109..3621
Translation	MFFYLSKKISIPNNVKLQCVSWNKEQGFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLSMNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKDLKGIQLSHVTWSADSKVLLFGMANGEIHIYDNQGNFMIKMKLSCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAVCFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHMGSVLAVAGFQKAAMQDKDVNIVQFYTPFGEHLGTLKVPGKEISALSWEGGGLKIALAVDSFIYFANIRPNYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKGLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDIVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDTPSGSMDGVLDYSKTIQGTRDPICAITASDKILIVGRESGTIQRYSLPNVGLIQKYSLNCRAYQLSLNCNSSRLAIIDISGVLTFFDLDARVTDSTGQQVVGELLKLERRDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPNKDYLINFEIRSLRDSRALIEKVGIKDASQFIEDNPHPRLWRLLAEAALQKLDLYTAEQAFVRCKDYQGIKFVKRLGKLLSESMKQAEVVGYFGRFEEAERTYLEMDRRDLAIGLRLKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGDYFADRQKWLNAVQYYVQGRNQERLAECYYMLEDYEGLENLAISLPENHKLLPEIAQMFVRVGMCEQAVTAFLKCSQPKAAVDTCVHLNQWNKAVELAKNHSMKEIGSLLARYASHLLEKNKTLDAIELYRKANYFFDAAKLMFKIADEEAKKGSKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKSSEATSALAGLLEEEVLSTTDRFTDNAWRGAEAYHFFILAQRQLYEGCVDTALKTALHLKDYEDIIPPVEIYSLLALCACASRAFGTCSKAFIKLKSLETLSSEQKQQYEDLALEIFTKHTSKDNRKPELDSLMEGGEGKLPTCVATGSPITEYQFWMCSVCKHGVLAQEISHYSFCPLCHSPVG

Target ORF information:

RefSeq Version	NM_020779.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020779.3

ORF Insert Sequence:

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGTTTGTA 
CTAGTTCTTT GTAATTCTAT TGGTACACCC TTGGATCCCA AATACATTGA TATTGTACCA 
TTGTTTGTTG CAATGACCAA AACCCATGTG ATAGCAGCCT CGAAAGAAGC ATTTTATACC 
TGGCAATATC GTGTGGCAAA GAAGCTCACA GCATTGGAAA TTAATCAGAT CACACGGTCT 
CGAAAAGAAG GGAGAGAAAG AATTTATCAT GTTGATGATA CCCCTTCTGG ATCAATGGAT 
GGTGTGCTTG ATTATAGTAA AACCATTCAA GGCACAAGGG ATCCAATTTG TGCCATAACT 
GCATCAGATA AGATATTGAT TGTGGGTCGT GAATCTGGCA CCATTCAGAG ATACAGTCTA 
CCTAATGTTG GTTTGATTCA AAAATATTCC CTTAATTGTC GAGCCTACCA GTTATCCTTG 
AATTGCAACT CTAGCCGTCT TGCTATCATA GACATCTCAG GAGTTCTGAC TTTCTTTGAC 
TTGGATGCTC GAGTAACGGA CAGTACGGGA CAGCAAGTAG TTGGAGAGTT GTTAAAATTG 
GAACGAAGAG ATGTCTGGGA TATGAAGTGG GCCAAAGATA ATCCTGATTT GTTTGCAATG 
ATGGAGAAGA CAAGAATGTA TGTTTTCAGA AACTTGGATC CTGAGGAACC CATTCAGACC 
TCTGGATATA TTTGTAATTT TGAGGATTTA GAAATTAAAT CTGTTCTTTT GGATGAGATA 
TTAAAGGATC CAGAACATCC AAACAAGGAT TACCTAATTA ACTTTGAGAT TCGGTCTCTG 
CGAGATAGCC GAGCACTGAT TGAGAAGGTT GGAATTAAAG ATGCATCTCA GTTCATAGAG 
GACAATCCAC ACCCCCGACT TTGGCGCCTA CTGGCTGAAG CAGCTCTTCA GAAACTGGAT 
CTATACACTG CAGAGCAAGC ATTTGTGCGC TGCAAAGATT ACCAAGGCAT TAAGTTTGTG 
AAGCGCTTGG GCAAACTACT GAGTGAGTCA ATGAAACAGG CTGAAGTTGT TGGCTACTTC 
GGCAGGTTTG AAGAGGCTGA AAGAACGTAT CTCGAGATGG ACAGAAGGGA TCTTGCTATT 
GGCCTCCGGC TGAAATTGGG GGATTGGTTT AGAGTACTCC AGCTCCTGAA AACTGGATCT 
GGTGATGCAG ATGACAGTCT CCTGGAACAA GCCAACAATG CCATTGGAGA CTACTTTGCT 
GATCGACAAA AGTGGTTGAA TGCTGTACAA TATTATGTAC AAGGACGGAA CCAGGAACGC 
TTAGCTGAAT GTTACTATAT GTTAGAGGAT TATGAAGGGT TAGAGAACCT TGCCATTTCA 
CTTCCAGAAA ACCACAAGTT ACTTCCAGAA ATAGCACAAA TGTTTGTCAG AGTTGGAATG 
TGTGAACAAG CAGTGACTGC ATTTTTGAAA TGTAGTCAAC CAAAGGCAGC AGTAGATACC 
TGCGTACATC TCAACCAATG GAACAAAGCT GTTGAATTGG CTAAAAATCA TAGTATGAAA 
GAAATTGGAT CTCTGTTAGC TAGGTATGCA TCTCATTTAC TGGAAAAGAA TAAAACTCTT 
GATGCCATAG AACTCTATCG GAAAGCCAAT TACTTTTTTG ATGCAGCTAA ACTGATGTTT 
AAGATTGCAG ATGAAGAGGC AAAGAAAGGA AGTAAACCTT TACGTGTCAA GAAGCTCTAT 
GTACTGTCAG CCTTACTTAT AGAGCAATAC CATGAACAGA TGAAGAATGC CCAGCGAGGA 
AAAGTTAAAG GAAAAAGTTC AGAGGCCACT TCTGCCTTGG CTGGTTTGCT GGAAGAAGAA 
GTTCTGTCTA CAACAGATCG TTTCACAGAT AATGCATGGA GAGGGGCAGA GGCTTACCAC 
TTCTTTATAC TTGCACAGAG GCAGCTCTAT GAGGGATGTG TGGACACTGC ACTGAAGACA 
GCTCTTCACC TGAAAGACTA TGAAGACATC ATCCCTCCTG TGGAGATCTA CTCTCTGCTA 
GCACTCTGCG CATGCGCCAG CAGAGCCTTT GGGACTTGTT CAAAAGCTTT CATTAAACTT 
AAATCTTTAG AGACCCTCAG TTCAGAACAG AAACAGCAGT ATGAAGACCT TGCTTTAGAA 
ATCTTCACCA AACATACTTC AAAAGATAAC AGAAAACCTG AATTGGACAG CCTTATGGAA 
GGTGGAGAAG GGAAACTGCC AACATGCGTT GCCACAGGAA GCCCAATCAC TGAGTATCAA 
TTCTGGATGT GCAGTGTATG CAAACACGGT GTCCTTGCTC AGGAAATAAG CCACTACAGC 
TTCTGCCCCT TATGCCATAG TCCAGTGGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WDR35 ( NM_020779.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065830.2 Homo sapiens WD repeat domain 35 (WDR35), transcript variant 2, mRNA.

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CloneID	OHu18950
Clone ID Related Accession (Same CDS sequence)	NM_020779.3 , NM_020779.4
Accession Version	NM_020779.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	WD repeat-containing protein 35 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036659.2, AB037757.2, BX476609.1 and BC033951.1. On Jun 2, 2019 this sequence version replaced NM_020779.3. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform 2 thus lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.221908.1, SRR1803613.144931.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGTTTGTA 
CTAGTTCTTT GTAATTCTAT TGGTACACCC TTGGATCCCA AATACATTGA TATTGTACCA 
TTGTTTGTTG CAATGACCAA AACCCATGTG ATAGCAGCCT CGAAAGAAGC ATTTTATACC 
TGGCAATATC GTGTGGCAAA GAAGCTCACA GCATTGGAAA TTAATCAGAT CACACGGTCT 
CGAAAAGAAG GGAGAGAAAG AATTTATCAT GTTGATGATA CCCCTTCTGG ATCAATGGAT 
GGTGTGCTTG ATTATAGTAA AACCATTCAA GGCACAAGGG ATCCAATTTG TGCCATAACT 
GCATCAGATA AGATATTGAT TGTGGGTCGT GAATCTGGCA CCATTCAGAG ATACAGTCTA 
CCTAATGTTG GTTTGATTCA AAAATATTCC CTTAATTGTC GAGCCTACCA GTTATCCTTG 
AATTGCAACT CTAGCCGTCT TGCTATCATA GACATCTCAG GAGTTCTGAC TTTCTTTGAC 
TTGGATGCTC GAGTAACGGA CAGTACGGGA CAGCAAGTAG TTGGAGAGTT GTTAAAATTG 
GAACGAAGAG ATGTCTGGGA TATGAAGTGG GCCAAAGATA ATCCTGATTT GTTTGCAATG 
ATGGAGAAGA CAAGAATGTA TGTTTTCAGA AACTTGGATC CTGAGGAACC CATTCAGACC 
TCTGGATATA TTTGTAATTT TGAGGATTTA GAAATTAAAT CTGTTCTTTT GGATGAGATA 
TTAAAGGATC CAGAACATCC AAACAAGGAT TACCTAATTA ACTTTGAGAT TCGGTCTCTG 
CGAGATAGCC GAGCACTGAT TGAGAAGGTT GGAATTAAAG ATGCATCTCA GTTCATAGAG 
GACAATCCAC ACCCCCGACT TTGGCGCCTA CTGGCTGAAG CAGCTCTTCA GAAACTGGAT 
CTATACACTG CAGAGCAAGC ATTTGTGCGC TGCAAAGATT ACCAAGGCAT TAAGTTTGTG 
AAGCGCTTGG GCAAACTACT GAGTGAGTCA ATGAAACAGG CTGAAGTTGT TGGCTACTTC 
GGCAGGTTTG AAGAGGCTGA AAGAACGTAT CTCGAGATGG ACAGAAGGGA TCTTGCTATT 
GGCCTCCGGC TGAAATTGGG GGATTGGTTT AGAGTACTCC AGCTCCTGAA AACTGGATCT 
GGTGATGCAG ATGACAGTCT CCTGGAACAA GCCAACAATG CCATTGGAGA CTACTTTGCT 
GATCGACAAA AGTGGTTGAA TGCTGTACAA TATTATGTAC AAGGACGGAA CCAGGAACGC 
TTAGCTGAAT GTTACTATAT GTTAGAGGAT TATGAAGGGT TAGAGAACCT TGCCATTTCA 
CTTCCAGAAA ACCACAAGTT ACTTCCAGAA ATAGCACAAA TGTTTGTCAG AGTTGGAATG 
TGTGAACAAG CAGTGACTGC ATTTTTGAAA TGTAGTCAAC CAAAGGCAGC AGTAGATACC 
TGCGTACATC TCAACCAATG GAACAAAGCT GTTGAATTGG CTAAAAATCA TAGTATGAAA 
GAAATTGGAT CTCTGTTAGC TAGGTATGCA TCTCATTTAC TGGAAAAGAA TAAAACTCTT 
GATGCCATAG AACTCTATCG GAAAGCCAAT TACTTTTTTG ATGCAGCTAA ACTGATGTTT 
AAGATTGCAG ATGAAGAGGC AAAGAAAGGA AGTAAACCTT TACGTGTCAA GAAGCTCTAT 
GTACTGTCAG CCTTACTTAT AGAGCAATAC CATGAACAGA TGAAGAATGC CCAGCGAGGA 
AAAGTTAAAG GAAAAAGTTC AGAGGCCACT TCTGCCTTGG CTGGTTTGCT GGAAGAAGAA 
GTTCTGTCTA CAACAGATCG TTTCACAGAT AATGCATGGA GAGGGGCAGA GGCTTACCAC 
TTCTTTATAC TTGCACAGAG GCAGCTCTAT GAGGGATGTG TGGACACTGC ACTGAAGACA 
GCTCTTCACC TGAAAGACTA TGAAGACATC ATCCCTCCTG TGGAGATCTA CTCTCTGCTA 
GCACTCTGCG CATGCGCCAG CAGAGCCTTT GGGACTTGTT CAAAAGCTTT CATTAAACTT 
AAATCTTTAG AGACCCTCAG TTCAGAACAG AAACAGCAGT ATGAAGACCT TGCTTTAGAA 
ATCTTCACCA AACATACTTC AAAAGATAAC AGAAAACCTG AATTGGACAG CCTTATGGAA 
GGTGGAGAAG GGAAACTGCC AACATGCGTT GCCACAGGAA GCCCAATCAC TGAGTATCAA 
TTCTGGATGT GCAGTGTATG CAAACACGGT GTCCTTGCTC AGGAAATAAG CCACTACAGC 
TTCTGCCCCT TATGCCATAG TCCAGTGGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065830.2
CDS	91..3603
Translation	MFFYLSKKISIPNNVKLQCVSWNKEQGFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLSMNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKDLKGIQLSHVTWSADSKVLLFGMANGEIHIYDNQGNFMIKMKLSCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAVCFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHMGSVLAVAGFQKAAMQDKDVNIVQFYTPFGEHLGTLKVPGKEISALSWEGGGLKIALAVDSFIYFANIRPNYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKGLISITTCGDFCILATKADENHPQFVLVLCNSIGTPLDPKYIDIVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDTPSGSMDGVLDYSKTIQGTRDPICAITASDKILIVGRESGTIQRYSLPNVGLIQKYSLNCRAYQLSLNCNSSRLAIIDISGVLTFFDLDARVTDSTGQQVVGELLKLERRDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPNKDYLINFEIRSLRDSRALIEKVGIKDASQFIEDNPHPRLWRLLAEAALQKLDLYTAEQAFVRCKDYQGIKFVKRLGKLLSESMKQAEVVGYFGRFEEAERTYLEMDRRDLAIGLRLKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGDYFADRQKWLNAVQYYVQGRNQERLAECYYMLEDYEGLENLAISLPENHKLLPEIAQMFVRVGMCEQAVTAFLKCSQPKAAVDTCVHLNQWNKAVELAKNHSMKEIGSLLARYASHLLEKNKTLDAIELYRKANYFFDAAKLMFKIADEEAKKGSKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKSSEATSALAGLLEEEVLSTTDRFTDNAWRGAEAYHFFILAQRQLYEGCVDTALKTALHLKDYEDIIPPVEIYSLLALCACASRAFGTCSKAFIKLKSLETLSSEQKQQYEDLALEIFTKHTSKDNRKPELDSLMEGGEGKLPTCVATGSPITEYQFWMCSVCKHGVLAQEISHYSFCPLCHSPVG

Target ORF information:

RefSeq Version	NM_020779.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020779.4

ORF Insert Sequence:

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGTTTGTA 
CTAGTTCTTT GTAATTCTAT TGGTACACCC TTGGATCCCA AATACATTGA TATTGTACCA 
TTGTTTGTTG CAATGACCAA AACCCATGTG ATAGCAGCCT CGAAAGAAGC ATTTTATACC 
TGGCAATATC GTGTGGCAAA GAAGCTCACA GCATTGGAAA TTAATCAGAT CACACGGTCT 
CGAAAAGAAG GGAGAGAAAG AATTTATCAT GTTGATGATA CCCCTTCTGG ATCAATGGAT 
GGTGTGCTTG ATTATAGTAA AACCATTCAA GGCACAAGGG ATCCAATTTG TGCCATAACT 
GCATCAGATA AGATATTGAT TGTGGGTCGT GAATCTGGCA CCATTCAGAG ATACAGTCTA 
CCTAATGTTG GTTTGATTCA AAAATATTCC CTTAATTGTC GAGCCTACCA GTTATCCTTG 
AATTGCAACT CTAGCCGTCT TGCTATCATA GACATCTCAG GAGTTCTGAC TTTCTTTGAC 
TTGGATGCTC GAGTAACGGA CAGTACGGGA CAGCAAGTAG TTGGAGAGTT GTTAAAATTG 
GAACGAAGAG ATGTCTGGGA TATGAAGTGG GCCAAAGATA ATCCTGATTT GTTTGCAATG 
ATGGAGAAGA CAAGAATGTA TGTTTTCAGA AACTTGGATC CTGAGGAACC CATTCAGACC 
TCTGGATATA TTTGTAATTT TGAGGATTTA GAAATTAAAT CTGTTCTTTT GGATGAGATA 
TTAAAGGATC CAGAACATCC AAACAAGGAT TACCTAATTA ACTTTGAGAT TCGGTCTCTG 
CGAGATAGCC GAGCACTGAT TGAGAAGGTT GGAATTAAAG ATGCATCTCA GTTCATAGAG 
GACAATCCAC ACCCCCGACT TTGGCGCCTA CTGGCTGAAG CAGCTCTTCA GAAACTGGAT 
CTATACACTG CAGAGCAAGC ATTTGTGCGC TGCAAAGATT ACCAAGGCAT TAAGTTTGTG 
AAGCGCTTGG GCAAACTACT GAGTGAGTCA ATGAAACAGG CTGAAGTTGT TGGCTACTTC 
GGCAGGTTTG AAGAGGCTGA AAGAACGTAT CTCGAGATGG ACAGAAGGGA TCTTGCTATT 
GGCCTCCGGC TGAAATTGGG GGATTGGTTT AGAGTACTCC AGCTCCTGAA AACTGGATCT 
GGTGATGCAG ATGACAGTCT CCTGGAACAA GCCAACAATG CCATTGGAGA CTACTTTGCT 
GATCGACAAA AGTGGTTGAA TGCTGTACAA TATTATGTAC AAGGACGGAA CCAGGAACGC 
TTAGCTGAAT GTTACTATAT GTTAGAGGAT TATGAAGGGT TAGAGAACCT TGCCATTTCA 
CTTCCAGAAA ACCACAAGTT ACTTCCAGAA ATAGCACAAA TGTTTGTCAG AGTTGGAATG 
TGTGAACAAG CAGTGACTGC ATTTTTGAAA TGTAGTCAAC CAAAGGCAGC AGTAGATACC 
TGCGTACATC TCAACCAATG GAACAAAGCT GTTGAATTGG CTAAAAATCA TAGTATGAAA 
GAAATTGGAT CTCTGTTAGC TAGGTATGCA TCTCATTTAC TGGAAAAGAA TAAAACTCTT 
GATGCCATAG AACTCTATCG GAAAGCCAAT TACTTTTTTG ATGCAGCTAA ACTGATGTTT 
AAGATTGCAG ATGAAGAGGC AAAGAAAGGA AGTAAACCTT TACGTGTCAA GAAGCTCTAT 
GTACTGTCAG CCTTACTTAT AGAGCAATAC CATGAACAGA TGAAGAATGC CCAGCGAGGA 
AAAGTTAAAG GAAAAAGTTC AGAGGCCACT TCTGCCTTGG CTGGTTTGCT GGAAGAAGAA 
GTTCTGTCTA CAACAGATCG TTTCACAGAT AATGCATGGA GAGGGGCAGA GGCTTACCAC 
TTCTTTATAC TTGCACAGAG GCAGCTCTAT GAGGGATGTG TGGACACTGC ACTGAAGACA 
GCTCTTCACC TGAAAGACTA TGAAGACATC ATCCCTCCTG TGGAGATCTA CTCTCTGCTA 
GCACTCTGCG CATGCGCCAG CAGAGCCTTT GGGACTTGTT CAAAAGCTTT CATTAAACTT 
AAATCTTTAG AGACCCTCAG TTCAGAACAG AAACAGCAGT ATGAAGACCT TGCTTTAGAA 
ATCTTCACCA AACATACTTC AAAAGATAAC AGAAAACCTG AATTGGACAG CCTTATGGAA 
GGTGGAGAAG GGAAACTGCC AACATGCGTT GCCACAGGAA GCCCAATCAC TGAGTATCAA 
TTCTGGATGT GCAGTGTATG CAAACACGGT GTCCTTGCTC AGGAAATAAG CCACTACAGC 
TTCTGCCCCT TATGCCATAG TCCAGTGGGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WDR35 ( NM_001006657.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001006658.1 Homo sapiens WD repeat domain 35 (WDR35), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19673
Clone ID Related Accession (Same CDS sequence)	NM_001006657.1 , NM_001006657.2
Accession Version	NM_001006657.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	WD repeat-containing protein 35 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB037757.2, BC036659.2, BX476609.1 and BC033951.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB037757.2, SRR1660809.52984.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGGAGGAG 
AACGAGATGG AGACATTTGG TGCAACGTTT GTACTAGTTC TTTGTAATTC TATTGGTACA 
CCCTTGGATC CCAAATACAT TGATATTGTA CCATTGTTTG TTGCAATGAC CAAAACCCAT 
GTGATAGCAG CCTCGAAAGA AGCATTTTAT ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC 
ACAGCATTGG AAATTAATCA GATCACACGG TCTCGAAAAG AAGGGAGAGA AAGAATTTAT 
CATGTTGATG ATACCCCTTC TGGATCAATG GATGGTGTGC TTGATTATAG TAAAACCATT 
CAAGGCACAA GGGATCCAAT TTGTGCCATA ACTGCATCAG ATAAGATATT GATTGTGGGT 
CGTGAATCTG GCACCATTCA GAGATACAGT CTACCTAATG TTGGTTTGAT TCAAAAATAT 
TCCCTTAATT GTCGAGCCTA CCAGTTATCC TTGAATTGCA ACTCTAGCCG TCTTGCTATC 
ATAGACATCT CAGGAGTTCT GACTTTCTTT GACTTGGATG CTCGAGTAAC GGACAGTACG 
GGACAGCAAG TAGTTGGAGA GTTGTTAAAA TTGGAACGAA GAGATGTCTG GGATATGAAG 
TGGGCCAAAG ATAATCCTGA TTTGTTTGCA ATGATGGAGA AGACAAGAAT GTATGTTTTC 
AGAAACTTGG ATCCTGAGGA ACCCATTCAG ACCTCTGGAT ATATTTGTAA TTTTGAGGAT 
TTAGAAATTA AATCTGTTCT TTTGGATGAG ATATTAAAGG ATCCAGAACA TCCAAACAAG 
GATTACCTAA TTAACTTTGA GATTCGGTCT CTGCGAGATA GCCGAGCACT GATTGAGAAG 
GTTGGAATTA AAGATGCATC TCAGTTCATA GAGGACAATC CACACCCCCG ACTTTGGCGC 
CTACTGGCTG AAGCAGCTCT TCAGAAACTG GATCTATACA CTGCAGAGCA AGCATTTGTG 
CGCTGCAAAG ATTACCAAGG CATTAAGTTT GTGAAGCGCT TGGGCAAACT ACTGAGTGAG 
TCAATGAAAC AGGCTGAAGT TGTTGGCTAC TTCGGCAGGT TTGAAGAGGC TGAAAGAACG 
TATCTCGAGA TGGACAGAAG GGATCTTGCT ATTGGCCTCC GGCTGAAATT GGGGGATTGG 
TTTAGAGTAC TCCAGCTCCT GAAAACTGGA TCTGGTGATG CAGATGACAG TCTCCTGGAA 
CAAGCCAACA ATGCCATTGG AGACTACTTT GCTGATCGAC AAAAGTGGTT GAATGCTGTA 
CAATATTATG TACAAGGACG GAACCAGGAA CGCTTAGCTG AATGTTACTA TATGTTAGAG 
GATTATGAAG GGTTAGAGAA CCTTGCCATT TCACTTCCAG AAAACCACAA GTTACTTCCA 
GAAATAGCAC AAATGTTTGT CAGAGTTGGA ATGTGTGAAC AAGCAGTGAC TGCATTTTTG 
AAATGTAGTC AACCAAAGGC AGCAGTAGAT ACCTGCGTAC ATCTCAACCA ATGGAACAAA 
GCTGTTGAAT TGGCTAAAAA TCATAGTATG AAAGAAATTG GATCTCTGTT AGCTAGGTAT 
GCATCTCATT TACTGGAAAA GAATAAAACT CTTGATGCCA TAGAACTCTA TCGGAAAGCC 
AATTACTTTT TTGATGCAGC TAAACTGATG TTTAAGATTG CAGATGAAGA GGCAAAGAAA 
GGAAGTAAAC CTTTACGTGT CAAGAAGCTC TATGTACTGT CAGCCTTACT TATAGAGCAA 
TACCATGAAC AGATGAAGAA TGCCCAGCGA GGAAAAGTTA AAGGAAAAAG TTCAGAGGCC 
ACTTCTGCCT TGGCTGGTTT GCTGGAAGAA GAAGTTCTGT CTACAACAGA TCGTTTCACA 
GATAATGCAT GGAGAGGGGC AGAGGCTTAC CACTTCTTTA TACTTGCACA GAGGCAGCTC 
TATGAGGGAT GTGTGGACAC TGCACTGAAG ACAGCTCTTC ACCTGAAAGA CTATGAAGAC 
ATCATCCCTC CTGTGGAGAT CTACTCTCTG CTAGCACTCT GCGCATGCGC CAGCAGAGCC 
TTTGGGACTT GTTCAAAAGC TTTCATTAAA CTTAAATCTT TAGAGACCCT CAGTTCAGAA 
CAGAAACAGC AGTATGAAGA CCTTGCTTTA GAAATCTTCA CCAAACATAC TTCAAAAGAT 
AACAGAAAAC CTGAATTGGA CAGCCTTATG GAAGGTGGAG AAGGGAAACT GCCAACATGC 
GTTGCCACAG GAAGCCCAAT CACTGAGTAT CAATTCTGGA TGTGCAGTGT ATGCAAACAC 
GGTGTCCTTG CTCAGGAAAT AAGCCACTAC AGCTTCTGCC CCTTATGCCA TAGTCCAGTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006658.1
CDS	109..3654
Translation	MFFYLSKKISIPNNVKLQCVSWNKEQGFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLSMNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKDLKGIQLSHVTWSADSKVLLFGMANGEIHIYDNQGNFMIKMKLSCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAVCFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHMGSVLAVAGFQKAAMQDKDVNIVQFYTPFGEHLGTLKVPGKEISALSWEGGGLKIALAVDSFIYFANIRPNYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKGLISITTCGDFCILATKADENHPQEENEMETFGATFVLVLCNSIGTPLDPKYIDIVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDTPSGSMDGVLDYSKTIQGTRDPICAITASDKILIVGRESGTIQRYSLPNVGLIQKYSLNCRAYQLSLNCNSSRLAIIDISGVLTFFDLDARVTDSTGQQVVGELLKLERRDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPNKDYLINFEIRSLRDSRALIEKVGIKDASQFIEDNPHPRLWRLLAEAALQKLDLYTAEQAFVRCKDYQGIKFVKRLGKLLSESMKQAEVVGYFGRFEEAERTYLEMDRRDLAIGLRLKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGDYFADRQKWLNAVQYYVQGRNQERLAECYYMLEDYEGLENLAISLPENHKLLPEIAQMFVRVGMCEQAVTAFLKCSQPKAAVDTCVHLNQWNKAVELAKNHSMKEIGSLLARYASHLLEKNKTLDAIELYRKANYFFDAAKLMFKIADEEAKKGSKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKSSEATSALAGLLEEEVLSTTDRFTDNAWRGAEAYHFFILAQRQLYEGCVDTALKTALHLKDYEDIIPPVEIYSLLALCACASRAFGTCSKAFIKLKSLETLSSEQKQQYEDLALEIFTKHTSKDNRKPELDSLMEGGEGKLPTCVATGSPITEYQFWMCSVCKHGVLAQEISHYSFCPLCHSPVG

Target ORF information:

RefSeq Version	NM_001006657.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006657.1

ORF Insert Sequence:

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGGAGGAG 
AACGAGATGG AGACATTTGG TGCAACGTTT GTACTAGTTC TTTGTAATTC TATTGGTACA 
CCCTTGGATC CCAAATACAT TGATATTGTA CCATTGTTTG TTGCAATGAC CAAAACCCAT 
GTGATAGCAG CCTCGAAAGA AGCATTTTAT ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC 
ACAGCATTGG AAATTAATCA GATCACACGG TCTCGAAAAG AAGGGAGAGA AAGAATTTAT 
CATGTTGATG ATACCCCTTC TGGATCAATG GATGGTGTGC TTGATTATAG TAAAACCATT 
CAAGGCACAA GGGATCCAAT TTGTGCCATA ACTGCATCAG ATAAGATATT GATTGTGGGT 
CGTGAATCTG GCACCATTCA GAGATACAGT CTACCTAATG TTGGTTTGAT TCAAAAATAT 
TCCCTTAATT GTCGAGCCTA CCAGTTATCC TTGAATTGCA ACTCTAGCCG TCTTGCTATC 
ATAGACATCT CAGGAGTTCT GACTTTCTTT GACTTGGATG CTCGAGTAAC GGACAGTACG 
GGACAGCAAG TAGTTGGAGA GTTGTTAAAA TTGGAACGAA GAGATGTCTG GGATATGAAG 
TGGGCCAAAG ATAATCCTGA TTTGTTTGCA ATGATGGAGA AGACAAGAAT GTATGTTTTC 
AGAAACTTGG ATCCTGAGGA ACCCATTCAG ACCTCTGGAT ATATTTGTAA TTTTGAGGAT 
TTAGAAATTA AATCTGTTCT TTTGGATGAG ATATTAAAGG ATCCAGAACA TCCAAACAAG 
GATTACCTAA TTAACTTTGA GATTCGGTCT CTGCGAGATA GCCGAGCACT GATTGAGAAG 
GTTGGAATTA AAGATGCATC TCAGTTCATA GAGGACAATC CACACCCCCG ACTTTGGCGC 
CTACTGGCTG AAGCAGCTCT TCAGAAACTG GATCTATACA CTGCAGAGCA AGCATTTGTG 
CGCTGCAAAG ATTACCAAGG CATTAAGTTT GTGAAGCGCT TGGGCAAACT ACTGAGTGAG 
TCAATGAAAC AGGCTGAAGT TGTTGGCTAC TTCGGCAGGT TTGAAGAGGC TGAAAGAACG 
TATCTCGAGA TGGACAGAAG GGATCTTGCT ATTGGCCTCC GGCTGAAATT GGGGGATTGG 
TTTAGAGTAC TCCAGCTCCT GAAAACTGGA TCTGGTGATG CAGATGACAG TCTCCTGGAA 
CAAGCCAACA ATGCCATTGG AGACTACTTT GCTGATCGAC AAAAGTGGTT GAATGCTGTA 
CAATATTATG TACAAGGACG GAACCAGGAA CGCTTAGCTG AATGTTACTA TATGTTAGAG 
GATTATGAAG GGTTAGAGAA CCTTGCCATT TCACTTCCAG AAAACCACAA GTTACTTCCA 
GAAATAGCAC AAATGTTTGT CAGAGTTGGA ATGTGTGAAC AAGCAGTGAC TGCATTTTTG 
AAATGTAGTC AACCAAAGGC AGCAGTAGAT ACCTGCGTAC ATCTCAACCA ATGGAACAAA 
GCTGTTGAAT TGGCTAAAAA TCATAGTATG AAAGAAATTG GATCTCTGTT AGCTAGGTAT 
GCATCTCATT TACTGGAAAA GAATAAAACT CTTGATGCCA TAGAACTCTA TCGGAAAGCC 
AATTACTTTT TTGATGCAGC TAAACTGATG TTTAAGATTG CAGATGAAGA GGCAAAGAAA 
GGAAGTAAAC CTTTACGTGT CAAGAAGCTC TATGTACTGT CAGCCTTACT TATAGAGCAA 
TACCATGAAC AGATGAAGAA TGCCCAGCGA GGAAAAGTTA AAGGAAAAAG TTCAGAGGCC 
ACTTCTGCCT TGGCTGGTTT GCTGGAAGAA GAAGTTCTGT CTACAACAGA TCGTTTCACA 
GATAATGCAT GGAGAGGGGC AGAGGCTTAC CACTTCTTTA TACTTGCACA GAGGCAGCTC 
TATGAGGGAT GTGTGGACAC TGCACTGAAG ACAGCTCTTC ACCTGAAAGA CTATGAAGAC 
ATCATCCCTC CTGTGGAGAT CTACTCTCTG CTAGCACTCT GCGCATGCGC CAGCAGAGCC 
TTTGGGACTT GTTCAAAAGC TTTCATTAAA CTTAAATCTT TAGAGACCCT CAGTTCAGAA 
CAGAAACAGC AGTATGAAGA CCTTGCTTTA GAAATCTTCA CCAAACATAC TTCAAAAGAT 
AACAGAAAAC CTGAATTGGA CAGCCTTATG GAAGGTGGAG AAGGGAAACT GCCAACATGC 
GTTGCCACAG GAAGCCCAAT CACTGAGTAT CAATTCTGGA TGTGCAGTGT ATGCAAACAC 
GGTGTCCTTG CTCAGGAAAT AAGCCACTAC AGCTTCTGCC CCTTATGCCA TAGTCCAGTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WDR35 ( NM_001006657.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001006658.1 Homo sapiens WD repeat domain 35 (WDR35), transcript variant 1, mRNA.

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CloneID	OHu19673
Clone ID Related Accession (Same CDS sequence)	NM_001006657.1 , NM_001006657.2
Accession Version	NM_001006657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	WD repeat-containing protein 35 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB037757.2, BC036659.2, BX476609.1 and BC033951.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001006657.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB037757.2, SRR1803613.27764.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGGAGGAG 
AACGAGATGG AGACATTTGG TGCAACGTTT GTACTAGTTC TTTGTAATTC TATTGGTACA 
CCCTTGGATC CCAAATACAT TGATATTGTA CCATTGTTTG TTGCAATGAC CAAAACCCAT 
GTGATAGCAG CCTCGAAAGA AGCATTTTAT ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC 
ACAGCATTGG AAATTAATCA GATCACACGG TCTCGAAAAG AAGGGAGAGA AAGAATTTAT 
CATGTTGATG ATACCCCTTC TGGATCAATG GATGGTGTGC TTGATTATAG TAAAACCATT 
CAAGGCACAA GGGATCCAAT TTGTGCCATA ACTGCATCAG ATAAGATATT GATTGTGGGT 
CGTGAATCTG GCACCATTCA GAGATACAGT CTACCTAATG TTGGTTTGAT TCAAAAATAT 
TCCCTTAATT GTCGAGCCTA CCAGTTATCC TTGAATTGCA ACTCTAGCCG TCTTGCTATC 
ATAGACATCT CAGGAGTTCT GACTTTCTTT GACTTGGATG CTCGAGTAAC GGACAGTACG 
GGACAGCAAG TAGTTGGAGA GTTGTTAAAA TTGGAACGAA GAGATGTCTG GGATATGAAG 
TGGGCCAAAG ATAATCCTGA TTTGTTTGCA ATGATGGAGA AGACAAGAAT GTATGTTTTC 
AGAAACTTGG ATCCTGAGGA ACCCATTCAG ACCTCTGGAT ATATTTGTAA TTTTGAGGAT 
TTAGAAATTA AATCTGTTCT TTTGGATGAG ATATTAAAGG ATCCAGAACA TCCAAACAAG 
GATTACCTAA TTAACTTTGA GATTCGGTCT CTGCGAGATA GCCGAGCACT GATTGAGAAG 
GTTGGAATTA AAGATGCATC TCAGTTCATA GAGGACAATC CACACCCCCG ACTTTGGCGC 
CTACTGGCTG AAGCAGCTCT TCAGAAACTG GATCTATACA CTGCAGAGCA AGCATTTGTG 
CGCTGCAAAG ATTACCAAGG CATTAAGTTT GTGAAGCGCT TGGGCAAACT ACTGAGTGAG 
TCAATGAAAC AGGCTGAAGT TGTTGGCTAC TTCGGCAGGT TTGAAGAGGC TGAAAGAACG 
TATCTCGAGA TGGACAGAAG GGATCTTGCT ATTGGCCTCC GGCTGAAATT GGGGGATTGG 
TTTAGAGTAC TCCAGCTCCT GAAAACTGGA TCTGGTGATG CAGATGACAG TCTCCTGGAA 
CAAGCCAACA ATGCCATTGG AGACTACTTT GCTGATCGAC AAAAGTGGTT GAATGCTGTA 
CAATATTATG TACAAGGACG GAACCAGGAA CGCTTAGCTG AATGTTACTA TATGTTAGAG 
GATTATGAAG GGTTAGAGAA CCTTGCCATT TCACTTCCAG AAAACCACAA GTTACTTCCA 
GAAATAGCAC AAATGTTTGT CAGAGTTGGA ATGTGTGAAC AAGCAGTGAC TGCATTTTTG 
AAATGTAGTC AACCAAAGGC AGCAGTAGAT ACCTGCGTAC ATCTCAACCA ATGGAACAAA 
GCTGTTGAAT TGGCTAAAAA TCATAGTATG AAAGAAATTG GATCTCTGTT AGCTAGGTAT 
GCATCTCATT TACTGGAAAA GAATAAAACT CTTGATGCCA TAGAACTCTA TCGGAAAGCC 
AATTACTTTT TTGATGCAGC TAAACTGATG TTTAAGATTG CAGATGAAGA GGCAAAGAAA 
GGAAGTAAAC CTTTACGTGT CAAGAAGCTC TATGTACTGT CAGCCTTACT TATAGAGCAA 
TACCATGAAC AGATGAAGAA TGCCCAGCGA GGAAAAGTTA AAGGAAAAAG TTCAGAGGCC 
ACTTCTGCCT TGGCTGGTTT GCTGGAAGAA GAAGTTCTGT CTACAACAGA TCGTTTCACA 
GATAATGCAT GGAGAGGGGC AGAGGCTTAC CACTTCTTTA TACTTGCACA GAGGCAGCTC 
TATGAGGGAT GTGTGGACAC TGCACTGAAG ACAGCTCTTC ACCTGAAAGA CTATGAAGAC 
ATCATCCCTC CTGTGGAGAT CTACTCTCTG CTAGCACTCT GCGCATGCGC CAGCAGAGCC 
TTTGGGACTT GTTCAAAAGC TTTCATTAAA CTTAAATCTT TAGAGACCCT CAGTTCAGAA 
CAGAAACAGC AGTATGAAGA CCTTGCTTTA GAAATCTTCA CCAAACATAC TTCAAAAGAT 
AACAGAAAAC CTGAATTGGA CAGCCTTATG GAAGGTGGAG AAGGGAAACT GCCAACATGC 
GTTGCCACAG GAAGCCCAAT CACTGAGTAT CAATTCTGGA TGTGCAGTGT ATGCAAACAC 
GGTGTCCTTG CTCAGGAAAT AAGCCACTAC AGCTTCTGCC CCTTATGCCA TAGTCCAGTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006658.1
CDS	91..3636
Translation	MFFYLSKKISIPNNVKLQCVSWNKEQGFIACGGEDGLLKVLKLETQTDDAKLRGLAAPSNLSMNQTLEGHSGSVQVVTWNEQYQKLTTSDENGLIIVWMLYKGSWIEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSVDGNRIWGKDLKGIQLSHVTWSADSKVLLFGMANGEIHIYDNQGNFMIKMKLSCLVNVTGAISIAGIHWYHGTEGYVEPDCPCLAVCFDNGRCQIMRHENDQNPVLIDTGMYVVGIQWNHMGSVLAVAGFQKAAMQDKDVNIVQFYTPFGEHLGTLKVPGKEISALSWEGGGLKIALAVDSFIYFANIRPNYKWGYCSNTVVYAYTRPDRPEYCVVFWDTKNNEKYVKYVKGLISITTCGDFCILATKADENHPQEENEMETFGATFVLVLCNSIGTPLDPKYIDIVPLFVAMTKTHVIAASKEAFYTWQYRVAKKLTALEINQITRSRKEGRERIYHVDDTPSGSMDGVLDYSKTIQGTRDPICAITASDKILIVGRESGTIQRYSLPNVGLIQKYSLNCRAYQLSLNCNSSRLAIIDISGVLTFFDLDARVTDSTGQQVVGELLKLERRDVWDMKWAKDNPDLFAMMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEILKDPEHPNKDYLINFEIRSLRDSRALIEKVGIKDASQFIEDNPHPRLWRLLAEAALQKLDLYTAEQAFVRCKDYQGIKFVKRLGKLLSESMKQAEVVGYFGRFEEAERTYLEMDRRDLAIGLRLKLGDWFRVLQLLKTGSGDADDSLLEQANNAIGDYFADRQKWLNAVQYYVQGRNQERLAECYYMLEDYEGLENLAISLPENHKLLPEIAQMFVRVGMCEQAVTAFLKCSQPKAAVDTCVHLNQWNKAVELAKNHSMKEIGSLLARYASHLLEKNKTLDAIELYRKANYFFDAAKLMFKIADEEAKKGSKPLRVKKLYVLSALLIEQYHEQMKNAQRGKVKGKSSEATSALAGLLEEEVLSTTDRFTDNAWRGAEAYHFFILAQRQLYEGCVDTALKTALHLKDYEDIIPPVEIYSLLALCACASRAFGTCSKAFIKLKSLETLSSEQKQQYEDLALEIFTKHTSKDNRKPELDSLMEGGEGKLPTCVATGSPITEYQFWMCSVCKHGVLAQEISHYSFCPLCHSPVG

Target ORF information:

RefSeq Version	NM_001006657.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain 35 (WDR35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006657.2

ORF Insert Sequence:

ATGTTCTTCT ACCTGAGCAA GAAAATTTCC ATTCCCAATA ACGTGAAGCT GCAGTGTGTA 
TCCTGGAACA AGGAACAAGG GTTCATAGCA TGCGGTGGTG AAGATGGATT ACTGAAAGTT 
TTGAAATTAG AGACGCAGAC AGATGATGCA AAATTGAGGG GCCTTGCAGC CCCCAGTAAC 
CTTTCTATGA ATCAGACTCT TGAAGGTCAT AGTGGTTCTG TTCAAGTTGT AACATGGAAT 
GAGCAGTATC AGAAGTTGAC TACCAGTGAT GAAAACGGGC TTATCATTGT GTGGATGTTA 
TATAAAGGCT CTTGGATTGA GGAGATGATC AACAATCGAA ATAAATCAGT TGTTCGCAGT 
ATGAGCTGGA ATGCTGACGG ACAGAAGATC TGCATTGTAT ATGAAGATGG GGCTGTGATA 
GTTGGTTCAG TGGATGGCAA TCGTATTTGG GGAAAAGACC TGAAGGGTAT ACAGCTATCC 
CATGTAACAT GGTCTGCGGA CAGTAAAGTC TTACTTTTTG GAATGGCAAA TGGGGAAATA 
CACATTTACG ATAATCAAGG AAATTTTATG ATAAAAATGA AACTGAGTTG TTTGGTGAAT 
GTCACTGGAG CTATCAGCAT TGCTGGAATT CATTGGTACC ATGGCACAGA AGGCTACGTG 
GAGCCTGATT GCCCTTGCCT TGCTGTTTGC TTTGATAATG GAAGATGCCA AATAATGAGA 
CATGAGAATG ACCAAAATCC CGTTTTGATT GACACTGGCA TGTACGTAGT AGGCATCCAG 
TGGAACCACA TGGGCAGCGT GTTAGCTGTG GCAGGCTTCC AGAAGGCAGC CATGCAGGAC 
AAAGATGTGA ACATTGTGCA GTTTTACACT CCGTTTGGTG AGCATCTGGG TACTTTGAAA 
GTTCCTGGAA AGGAAATATC TGCACTATCT TGGGAAGGAG GTGGACTGAA AATTGCACTA 
GCTGTTGATT CCTTTATATA TTTTGCAAAC ATTCGACCTA ATTATAAGTG GGGTTATTGC 
TCAAACACTG TAGTTTATGC ATATACCAGA CCTGATCGTC CAGAATATTG TGTTGTCTTC 
TGGGATACGA AAAACAATGA AAAATATGTT AAATATGTGA AGGGTCTCAT TTCTATTACT 
ACCTGTGGAG ATTTCTGCAT TTTGGCTACA AAAGCTGATG AAAATCATCC TCAGGAGGAG 
AACGAGATGG AGACATTTGG TGCAACGTTT GTACTAGTTC TTTGTAATTC TATTGGTACA 
CCCTTGGATC CCAAATACAT TGATATTGTA CCATTGTTTG TTGCAATGAC CAAAACCCAT 
GTGATAGCAG CCTCGAAAGA AGCATTTTAT ACCTGGCAAT ATCGTGTGGC AAAGAAGCTC 
ACAGCATTGG AAATTAATCA GATCACACGG TCTCGAAAAG AAGGGAGAGA AAGAATTTAT 
CATGTTGATG ATACCCCTTC TGGATCAATG GATGGTGTGC TTGATTATAG TAAAACCATT 
CAAGGCACAA GGGATCCAAT TTGTGCCATA ACTGCATCAG ATAAGATATT GATTGTGGGT 
CGTGAATCTG GCACCATTCA GAGATACAGT CTACCTAATG TTGGTTTGAT TCAAAAATAT 
TCCCTTAATT GTCGAGCCTA CCAGTTATCC TTGAATTGCA ACTCTAGCCG TCTTGCTATC 
ATAGACATCT CAGGAGTTCT GACTTTCTTT GACTTGGATG CTCGAGTAAC GGACAGTACG 
GGACAGCAAG TAGTTGGAGA GTTGTTAAAA TTGGAACGAA GAGATGTCTG GGATATGAAG 
TGGGCCAAAG ATAATCCTGA TTTGTTTGCA ATGATGGAGA AGACAAGAAT GTATGTTTTC 
AGAAACTTGG ATCCTGAGGA ACCCATTCAG ACCTCTGGAT ATATTTGTAA TTTTGAGGAT 
TTAGAAATTA AATCTGTTCT TTTGGATGAG ATATTAAAGG ATCCAGAACA TCCAAACAAG 
GATTACCTAA TTAACTTTGA GATTCGGTCT CTGCGAGATA GCCGAGCACT GATTGAGAAG 
GTTGGAATTA AAGATGCATC TCAGTTCATA GAGGACAATC CACACCCCCG ACTTTGGCGC 
CTACTGGCTG AAGCAGCTCT TCAGAAACTG GATCTATACA CTGCAGAGCA AGCATTTGTG 
CGCTGCAAAG ATTACCAAGG CATTAAGTTT GTGAAGCGCT TGGGCAAACT ACTGAGTGAG 
TCAATGAAAC AGGCTGAAGT TGTTGGCTAC TTCGGCAGGT TTGAAGAGGC TGAAAGAACG 
TATCTCGAGA TGGACAGAAG GGATCTTGCT ATTGGCCTCC GGCTGAAATT GGGGGATTGG 
TTTAGAGTAC TCCAGCTCCT GAAAACTGGA TCTGGTGATG CAGATGACAG TCTCCTGGAA 
CAAGCCAACA ATGCCATTGG AGACTACTTT GCTGATCGAC AAAAGTGGTT GAATGCTGTA 
CAATATTATG TACAAGGACG GAACCAGGAA CGCTTAGCTG AATGTTACTA TATGTTAGAG 
GATTATGAAG GGTTAGAGAA CCTTGCCATT TCACTTCCAG AAAACCACAA GTTACTTCCA 
GAAATAGCAC AAATGTTTGT CAGAGTTGGA ATGTGTGAAC AAGCAGTGAC TGCATTTTTG 
AAATGTAGTC AACCAAAGGC AGCAGTAGAT ACCTGCGTAC ATCTCAACCA ATGGAACAAA 
GCTGTTGAAT TGGCTAAAAA TCATAGTATG AAAGAAATTG GATCTCTGTT AGCTAGGTAT 
GCATCTCATT TACTGGAAAA GAATAAAACT CTTGATGCCA TAGAACTCTA TCGGAAAGCC 
AATTACTTTT TTGATGCAGC TAAACTGATG TTTAAGATTG CAGATGAAGA GGCAAAGAAA 
GGAAGTAAAC CTTTACGTGT CAAGAAGCTC TATGTACTGT CAGCCTTACT TATAGAGCAA 
TACCATGAAC AGATGAAGAA TGCCCAGCGA GGAAAAGTTA AAGGAAAAAG TTCAGAGGCC 
ACTTCTGCCT TGGCTGGTTT GCTGGAAGAA GAAGTTCTGT CTACAACAGA TCGTTTCACA 
GATAATGCAT GGAGAGGGGC AGAGGCTTAC CACTTCTTTA TACTTGCACA GAGGCAGCTC 
TATGAGGGAT GTGTGGACAC TGCACTGAAG ACAGCTCTTC ACCTGAAAGA CTATGAAGAC 
ATCATCCCTC CTGTGGAGAT CTACTCTCTG CTAGCACTCT GCGCATGCGC CAGCAGAGCC 
TTTGGGACTT GTTCAAAAGC TTTCATTAAA CTTAAATCTT TAGAGACCCT CAGTTCAGAA 
CAGAAACAGC AGTATGAAGA CCTTGCTTTA GAAATCTTCA CCAAACATAC TTCAAAAGAT 
AACAGAAAAC CTGAATTGGA CAGCCTTATG GAAGGTGGAG AAGGGAAACT GCCAACATGC 
GTTGCCACAG GAAGCCCAAT CACTGAGTAT CAATTCTGGA TGTGCAGTGT ATGCAAACAC 
GGTGTCCTTG CTCAGGAAAT AAGCCACTAC AGCTTCTGCC CCTTATGCCA TAGTCCAGTG 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.
Human molecular genetics(2017 Dec)
Takahara M,Katoh Y,Nakamura K,Hirano T,Sugawa M,Tsurumi Y,Nakayama K

Uncommon runs of homozygosity disclose homozygous missense mutations in two ciliopathy-related genes (SPAG17 and WDR35) in a patient with multiple brain and skeletal anomalies.
European journal of medical genetics(2017 Nov)
C?rdova-Fletes C,Becerra-Solano LE,Rangel-Sosa MM,Rivas-Estilla AM,Alberto Gal?n-Huerta K,Ortiz-L?pez R,Rojas-Mart?nez A,Ju?rez-V?zquez CI,Garc?a-Ortiz JE

Clinical and molecular genetic characterization of a male patient with Sensenbrenner syndrome (cranioectodermal dysplasia) and biallelic WDR35 mutations.
Birth defects research(2017 Nov)
Walczak-Sztulpa J,Wawrocka A,Swiader-Lesniak A,Socha M,Jamsheer A,Drozdz D,Latos-Bielenska A,Zachwieja K

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WDR35 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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