BAG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BAG1
Entrez Gene ID	573
Full Name	BCL2 associated athanogene 1
Synonyms	BAG-1,HAP,RAP46
General protein information	Preferred Names BCL2 associated athanogene 1 Names BAG family molecular chaperone regulator 1 BCL2-associated athanogene Bcl-2 associating athanogene-1 protein Bcl-2-binding protein glucocortoid receptor-associated protein RAP46 receptor-associated protein, 46-KD
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p13.3
Summary	The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010].
Disorder MIM:	601497

mRNA and Protein(s)

mRNA	Protein	Name
NM_001349286.1	NP_001336215.1	BAG family molecular chaperone regulator 1 isoform BAG-1M
NM_001172415.2	NP_001165886.1	BAG family molecular chaperone regulator 1 isoform BAG-1S
NM_001349299.2	NP_001336228.1	BAG family molecular chaperone regulator 1 isoform BAG1
NM_001349286.2	NP_001336215.1	BAG family molecular chaperone regulator 1 isoform BAG-1M
NM_001172415.1	NP_001165886.1	BAG family molecular chaperone regulator 1 isoform BAG-1S
NM_004323.5	NP_004314.5	BAG family molecular chaperone regulator 1 isoform BAG-1L
NM_004323.6	NP_004314.6	BAG family molecular chaperone regulator 1 isoform BAG-1L
NM_001349299.1	NP_001336228.1	BAG family molecular chaperone regulator 1 isoform BAG1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BAG1	XP_520528.3
Canis lupus familiaris (dog)	BAG1	XP_005627044.1
Bos taurus (cattle)	BAG1	NP_001069759.1
Rattus norvegicus (Norway rat)	Bag1	NP_001100117.2
	BAG1	XP_002800106.1
Gallus gallus (chicken)	BAG1	NP_001103162.1
Danio rerio (zebrafish)	bag1	NP_001092206.1
Xenopus tropicalis (tropical clawed frog)	bag1	XP_002939543.2
Mus musculus (house mouse)	Bag1	NP_033866.4
Homo sapiens (human)	BAG1	NP_004314.5

Gene Ontology
Bibliography

Related articles in PubMed

An increase in BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signalling.
Lin PL, Wu TC, Wu DW, Wang L, Chen CY, Lee H
European journal of cancer (Oxford, England : 1990)8595-105(2017 11)

Over-expression of BAG-1 in head and neck squamous cell carcinomas (HNSCC) is associated with cisplatin-resistance.
Liu S, Ren B, Gao H, Liao S, Zhai YX, Li S, Su XJ, Jin P, Stroncek D, Xu Z, Zeng Q, Li Y
Journal of translational medicine15(1)189(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signaling.
Title: An increase in BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signalling.

interaction with Bag1 then shifts hERG degradation to the membrane-anchored E3 ligase TRC8 and its E2-conjugating enzyme Ube2g2, as determined by siRNA screening
Title: Bag1 Co-chaperone Promotes TRC8 E3 Ligase-dependent Degradation of Misfolded Human Ether a Go-Go-related Gene (hERG) Potassium Channels.

These results suggest that Bag1 and Bag3 control the stability of the Hsc70-client complex using at least two distinct protein-protein contacts, providing a previously under-appreciated layer of molecular regulation in the human Hsc70 system.
Title: Non-canonical Interactions between Heat Shock Cognate Protein 70 (Hsc70) and Bcl2-associated Anthanogene (BAG) Co-Chaperones Are Important for Client Release.

BAG-4/SODD, HSP70, and HSP90 are potential prognostic markers in node-negative breast carcinoma that merit further research.
Title: BAG-1/SODD, HSP70, and HSP90 are potential prognostic markers of poor survival in node-negative breast carcinoma.

Bag-1 indirectly affects cell survival through c-Myc activated signalling that causes elevation of ornithine decarboxylase levels, leading to an increase of the polyamine content.
Title: Bag-1 promotes cell survival through c-Myc-mediated ODC upregulation that is not preferred under apoptotic stimuli in MCF-7 cells.

The following BAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22243	NM_001172415.2 Latest version!	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu22759	NM_004323.5 Latest version!	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu101294	NM_001349299.2 Latest version!	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu107270	NM_004323.6 Latest version!	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu101293	NM_001349286.2 Latest version!	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu101293	NM_001349286.1	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu22243	NM_001172415.1	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu101294	NM_001349299.1	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BAG1 ( NM_001172415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165886.1 Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22243
Clone ID Related Accession (Same CDS sequence)	NM_001172415.2 , NM_001172415.1
Accession Version	NM_001172415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG-1S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356472.17 and AL161445.10. On Jun 2, 2019 this sequence version replaced NM_001172415.1. Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. The most abundant of the shorter isoforms (BAG-1S, also known as p33 or p36), which is derived from the second downstream AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS ID represents the most abundant of the shorter human BAG1 isoforms, known as BAG-1S or p36 or p33, as described in the literature, including PMIDs 9396724, 9679980, 9747877 and 17662274. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at other AUG start codons produces additional isoforms, known as BAG-1M or p46, and p29. In addition, alternative translation initiation from an upstream non-AUG (CUG) start codon results in the longest human BAG1 isoform, known as BAG-1L or p50. The longest isoform is represented by CCDS 35004.1. Evidence in PMIDs 9747877 and 17662274 indicates that these isoforms have distinct subcellular distributions, which may contribute to the multifunctionality of the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222749.1, SRR1660805.3263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9396724) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATCGGA GCCAGGAGGT GACCCGGGAC GAGGAGTCGA CCCGGAGCGA GGAGGTGACC 
AGGGAGGAAA TGGCGGCAGC TGGGCTCACC GTGACTGTCA CCCACAGCAA TGAGAAGCAC 
GACCTTCATG TTACCTCCCA GCAGGGCAGC AGTGAACCAG TTGTCCAAGA CCTGGCCCAG 
GTTGTTGAAG AGGTCATAGG GGTTCCACAG TCTTTTCAGA AACTCATATT TAAGGGAAAA 
TCTCTGAAGG AAATGGAAAC ACCGTTGTCA GCACTTGGAA TACAAGATGG TTGCCGGGTC 
ATGTTAATTG GGAAAAAGAA CAGTCCACAG GAAGAGGTTG AACTAAAGAA GTTGAAACAT 
TTGGAGAAGT CTGTGGAGAA GATAGCTGAC CAGCTGGAAG AGTTGAATAA AGAGCTTACT 
GGAATCCAGC AGGGTTTTCT GCCCAAGGAT TTGCAAGCTG AAGCTCTCTG CAAACTTGAT 
AGGAGAGTAA AAGCCACAAT AGAGCAGTTT ATGAAGATCT TGGAGGAGAT TGACACACTG 
ATCCTGCCAG AAAATTTCAA AGACAGTAGA TTGAAAAGGA AAGGCTTGGT AAAAAAGGTT 
CAGGCATTCC TAGCCGAGTG TGACACAGTG GAGCAGAACA TCTGCCAGGA GACTGAGCGG 
CTGCAGTCTA CAAACTTTGC CCTGGCCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165886.1
CDS	380..1072
Translation	MNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_001172415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172415.2

ORF Insert Sequence:

ATGAATCGGA GCCAGGAGGT GACCCGGGAC GAGGAGTCGA CCCGGAGCGA GGAGGTGACC 
AGGGAGGAAA TGGCGGCAGC TGGGCTCACC GTGACTGTCA CCCACAGCAA TGAGAAGCAC 
GACCTTCATG TTACCTCCCA GCAGGGCAGC AGTGAACCAG TTGTCCAAGA CCTGGCCCAG 
GTTGTTGAAG AGGTCATAGG GGTTCCACAG TCTTTTCAGA AACTCATATT TAAGGGAAAA 
TCTCTGAAGG AAATGGAAAC ACCGTTGTCA GCACTTGGAA TACAAGATGG TTGCCGGGTC 
ATGTTAATTG GGAAAAAGAA CAGTCCACAG GAAGAGGTTG AACTAAAGAA GTTGAAACAT 
TTGGAGAAGT CTGTGGAGAA GATAGCTGAC CAGCTGGAAG AGTTGAATAA AGAGCTTACT 
GGAATCCAGC AGGGTTTTCT GCCCAAGGAT TTGCAAGCTG AAGCTCTCTG CAAACTTGAT 
AGGAGAGTAA AAGCCACAAT AGAGCAGTTT ATGAAGATCT TGGAGGAGAT TGACACACTG 
ATCCTGCCAG AAAATTTCAA AGACAGTAGA TTGAAAAGGA AAGGCTTGGT AAAAAAGGTT 
CAGGCATTCC TAGCCGAGTG TGACACAGTG GAGCAGAACA TCTGCCAGGA GACTGAGCGG 
CTGCAGTCTA CAAACTTTGC CCTGGCCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_001172415.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165886.1 Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22243
Clone ID Related Accession (Same CDS sequence)	NM_001172415.2 , NM_001172415.1
Accession Version	NM_001172415.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG-1S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG723775.1, BC001936.1, AL161445.10 and CN478628.1. Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. The most abundant of the shorter isoforms (BAG-1S, also known as p33 or p36), which is derived from the second downstream AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS ID represents the most abundant of the shorter human BAG1 isoforms, known as BAG-1S or p36 or p33, as described in the literature, including PMIDs 9396724, 9679980, 9747877 and 17662274. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at other AUG start codons produces additional isoforms, known as BAG-1M or p46, and p29. In addition, alternative translation initiation from an upstream non-AUG (CUG) start codon results in the longest human BAG1 isoform, known as BAG-1L or p50. The longest isoform is represented by CCDS 35004.1. Evidence in PMIDs 9747877 and 17662274 indicates that these isoforms have distinct subcellular distributions, which may contribute to the multifunctionality of the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.3263.1, SRR1803612.224516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9396724) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATCGGA GCCAGGAGGT GACCCGGGAC GAGGAGTCGA CCCGGAGCGA GGAGGTGACC 
AGGGAGGAAA TGGCGGCAGC TGGGCTCACC GTGACTGTCA CCCACAGCAA TGAGAAGCAC 
GACCTTCATG TTACCTCCCA GCAGGGCAGC AGTGAACCAG TTGTCCAAGA CCTGGCCCAG 
GTTGTTGAAG AGGTCATAGG GGTTCCACAG TCTTTTCAGA AACTCATATT TAAGGGAAAA 
TCTCTGAAGG AAATGGAAAC ACCGTTGTCA GCACTTGGAA TACAAGATGG TTGCCGGGTC 
ATGTTAATTG GGAAAAAGAA CAGTCCACAG GAAGAGGTTG AACTAAAGAA GTTGAAACAT 
TTGGAGAAGT CTGTGGAGAA GATAGCTGAC CAGCTGGAAG AGTTGAATAA AGAGCTTACT 
GGAATCCAGC AGGGTTTTCT GCCCAAGGAT TTGCAAGCTG AAGCTCTCTG CAAACTTGAT 
AGGAGAGTAA AAGCCACAAT AGAGCAGTTT ATGAAGATCT TGGAGGAGAT TGACACACTG 
ATCCTGCCAG AAAATTTCAA AGACAGTAGA TTGAAAAGGA AAGGCTTGGT AAAAAAGGTT 
CAGGCATTCC TAGCCGAGTG TGACACAGTG GAGCAGAACA TCTGCCAGGA GACTGAGCGG 
CTGCAGTCTA CAAACTTTGC CCTGGCCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165886.1
CDS	433..1125
Translation	MNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_001172415.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172415.1

ORF Insert Sequence:

ATGAATCGGA GCCAGGAGGT GACCCGGGAC GAGGAGTCGA CCCGGAGCGA GGAGGTGACC 
AGGGAGGAAA TGGCGGCAGC TGGGCTCACC GTGACTGTCA CCCACAGCAA TGAGAAGCAC 
GACCTTCATG TTACCTCCCA GCAGGGCAGC AGTGAACCAG TTGTCCAAGA CCTGGCCCAG 
GTTGTTGAAG AGGTCATAGG GGTTCCACAG TCTTTTCAGA AACTCATATT TAAGGGAAAA 
TCTCTGAAGG AAATGGAAAC ACCGTTGTCA GCACTTGGAA TACAAGATGG TTGCCGGGTC 
ATGTTAATTG GGAAAAAGAA CAGTCCACAG GAAGAGGTTG AACTAAAGAA GTTGAAACAT 
TTGGAGAAGT CTGTGGAGAA GATAGCTGAC CAGCTGGAAG AGTTGAATAA AGAGCTTACT 
GGAATCCAGC AGGGTTTTCT GCCCAAGGAT TTGCAAGCTG AAGCTCTCTG CAAACTTGAT 
AGGAGAGTAA AAGCCACAAT AGAGCAGTTT ATGAAGATCT TGGAGGAGAT TGACACACTG 
ATCCTGCCAG AAAATTTCAA AGACAGTAGA TTGAAAAGGA AAGGCTTGGT AAAAAAGGTT 
CAGGCATTCC TAGCCGAGTG TGACACAGTG GAGCAGAACA TCTGCCAGGA GACTGAGCGG 
CTGCAGTCTA CAAACTTTGC CCTGGCCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_004323.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004314.5 Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22759
Clone ID Related Accession (Same CDS sequence)	NM_004323.5
Accession Version	NM_004323.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG-1L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG723775.1, BC001936.1, AL161445.10 and CN478628.1. This sequence is a reference standard in the RefSeqGene project. On Feb 17, 2010 this sequence version replaced NM_004323.4. Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. The longest isoform (BAG-1L) is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS ID represents the longest human BAG1 isoform, known as BAG-1L or p50, as described in the literature, including PMIDs 9396724, 9679980, 9747877 and 17662274. This isoform initiates translation at a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation at downstream AUG start codons produces three additional isoforms with shorter N-termini, known as BAG-1M or p46, BAG-1S or p36 (also known as p33), and p29. The most abundant of the shorter isoforms, BAG-1S, is represented by CCDS 55301.1. Evidence in PMIDs 9747877 and 17662274 indicates that these isoforms have distinct subcellular distributions, which may contribute to the multifunctionality of the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.3263.1, SRR1803612.224516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 9679980, 9396724 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGGCTCAGC GCGGGGGGGC GCGGAGACCG CGAGGCGACC GGGAGCGGCT GGGTTCCCGG 
CTGCGCGCCC TTCGGCCAGG CCGGGAGCCG CGCCAGTCGG AGCCCCCGGC CCAGCGTGGT 
CCGCCTCCCT CTCGGCGTCC ACCTGCCCGG AGTACTGCCA GCGGGCATGA CCGACCCACC 
AGGGGCGCCG CCGCCGGCGC TCGCAGGCCG CGGATGAAGA AGAAAACCCG GCGCCGCTCG 
ACCCGGAGCG AGGAGTTGAC CCGGAGCGAG GAGTTGACCC TGAGTGAGGA AGCGACCTGG 
AGTGAAGAGG CGACCCAGAG TGAGGAGGCG ACCCAGGGCG AAGAGATGAA TCGGAGCCAG 
GAGGTGACCC GGGACGAGGA GTCGACCCGG AGCGAGGAGG TGACCAGGGA GGAAATGGCG 
GCAGCTGGGC TCACCGTGAC TGTCACCCAC AGCAATGAGA AGCACGACCT TCATGTTACC 
TCCCAGCAGG GCAGCAGTGA ACCAGTTGTC CAAGACCTGG CCCAGGTTGT TGAAGAGGTC 
ATAGGGGTTC CACAGTCTTT TCAGAAACTC ATATTTAAGG GAAAATCTCT GAAGGAAATG 
GAAACACCGT TGTCAGCACT TGGAATACAA GATGGTTGCC GGGTCATGTT AATTGGGAAA 
AAGAACAGTC CACAGGAAGA GGTTGAACTA AAGAAGTTGA AACATTTGGA GAAGTCTGTG 
GAGAAGATAG CTGACCAGCT GGAAGAGTTG AATAAAGAGC TTACTGGAAT CCAGCAGGGT 
TTTCTGCCCA AGGATTTGCA AGCTGAAGCT CTCTGCAAAC TTGATAGGAG AGTAAAAGCC 
ACAATAGAGC AGTTTATGAA GATCTTGGAG GAGATTGACA CACTGATCCT GCCAGAAAAT 
TTCAAAGACA GTAGATTGAA AAGGAAAGGC TTGGTAAAAA AGGTTCAGGC ATTCCTAGCC 
GAGTGTGACA CAGTGGAGCA GAACATCTGC CAGGAGACTG AGCGGCTGCA GTCTACAAAC 
TTTGCCCTGG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004314.5
CDS	88..1125
Translation	LAQRGGARRPRGDRERLGSRLRALRPGREPRQSEPPAQRGPPPSRRPPARSTASGHDRPTRGAAAGARRPRMKKKTRRRSTRSEELTRSEELTLSEEATWSEEATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_004323.5
Organism	Homo sapiens(human)
Definition	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004323.5

ORF Insert Sequence:

CTGGCTCAGC GCGGGGGGGC GCGGAGACCG CGAGGCGACC GGGAGCGGCT GGGTTCCCGG 
CTGCGCGCCC TTCGGCCAGG CCGGGAGCCG CGCCAGTCGG AGCCCCCGGC CCAGCGTGGT 
CCGCCTCCCT CTCGGCGTCC ACCTGCCCGG AGTACTGCCA GCGGGCATGA CCGACCCACC 
AGGGGCGCCG CCGCCGGCGC TCGCAGGCCG CGGATGAAGA AGAAAACCCG GCGCCGCTCG 
ACCCGGAGCG AGGAGTTGAC CCGGAGCGAG GAGTTGACCC TGAGTGAGGA AGCGACCTGG 
AGTGAAGAGG CGACCCAGAG TGAGGAGGCG ACCCAGGGCG AAGAGATGAA TCGGAGCCAG 
GAGGTGACCC GGGACGAGGA GTCGACCCGG AGCGAGGAGG TGACCAGGGA GGAAATGGCG 
GCAGCTGGGC TCACCGTGAC TGTCACCCAC AGCAATGAGA AGCACGACCT TCATGTTACC 
TCCCAGCAGG GCAGCAGTGA ACCAGTTGTC CAAGACCTGG CCCAGGTTGT TGAAGAGGTC 
ATAGGGGTTC CACAGTCTTT TCAGAAACTC ATATTTAAGG GAAAATCTCT GAAGGAAATG 
GAAACACCGT TGTCAGCACT TGGAATACAA GATGGTTGCC GGGTCATGTT AATTGGGAAA 
AAGAACAGTC CACAGGAAGA GGTTGAACTA AAGAAGTTGA AACATTTGGA GAAGTCTGTG 
GAGAAGATAG CTGACCAGCT GGAAGAGTTG AATAAAGAGC TTACTGGAAT CCAGCAGGGT 
TTTCTGCCCA AGGATTTGCA AGCTGAAGCT CTCTGCAAAC TTGATAGGAG AGTAAAAGCC 
ACAATAGAGC AGTTTATGAA GATCTTGGAG GAGATTGACA CACTGATCCT GCCAGAAAAT 
TTCAAAGACA GTAGATTGAA AAGGAAAGGC TTGGTAAAAA AGGTTCAGGC ATTCCTAGCC 
GAGTGTGACA CAGTGGAGCA GAACATCTGC CAGGAGACTG AGCGGCTGCA GTCTACAAAC 
TTTGCCCTGG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_001349299.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336228.1 Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu101294
Clone ID Related Accession (Same CDS sequence)	NM_001349299.2 , NM_001349299.1
Accession Version	NM_001349299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356472.17 and AL161445.10. On Jun 2, 2019 this sequence version replaced NM_001349299.1. Transcript Variant: Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. A minor, shorter isoform (BAG-1, also known as p29), is derived from the third downstream AUG start codon, and is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222749.1, SRR1660805.3263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9396724) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CTGGGCTCAC CGTGACTGTC ACCCACAGCA ATGAGAAGCA CGACCTTCAT 
GTTACCTCCC AGCAGGGCAG CAGTGAACCA GTTGTCCAAG ACCTGGCCCA GGTTGTTGAA 
GAGGTCATAG GGGTTCCACA GTCTTTTCAG AAACTCATAT TTAAGGGAAA ATCTCTGAAG 
GAAATGGAAA CACCGTTGTC AGCACTTGGA ATACAAGATG GTTGCCGGGT CATGTTAATT 
GGGAAAAAGA ACAGTCCACA GGAAGAGGTT GAACTAAAGA AGTTGAAACA TTTGGAGAAG 
TCTGTGGAGA AGATAGCTGA CCAGCTGGAA GAGTTGAATA AAGAGCTTAC TGGAATCCAG 
CAGGGTTTTC TGCCCAAGGA TTTGCAAGCT GAAGCTCTCT GCAAACTTGA TAGGAGAGTA 
AAAGCCACAA TAGAGCAGTT TATGAAGATC TTGGAGGAGA TTGACACACT GATCCTGCCA 
GAAAATTTCA AAGACAGTAG ATTGAAAAGG AAAGGCTTGG TAAAAAAGGT TCAGGCATTC 
CTAGCCGAGT GTGACACAGT GGAGCAGAAC ATCTGCCAGG AGACTGAGCG GCTGCAGTCT 
ACAAACTTTG CCCTGGCCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336228.1
CDS	449..1072
Translation	MAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_001349299.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349299.2

ORF Insert Sequence:

ATGGCGGCAG CTGGGCTCAC CGTGACTGTC ACCCACAGCA ATGAGAAGCA CGACCTTCAT 
GTTACCTCCC AGCAGGGCAG CAGTGAACCA GTTGTCCAAG ACCTGGCCCA GGTTGTTGAA 
GAGGTCATAG GGGTTCCACA GTCTTTTCAG AAACTCATAT TTAAGGGAAA ATCTCTGAAG 
GAAATGGAAA CACCGTTGTC AGCACTTGGA ATACAAGATG GTTGCCGGGT CATGTTAATT 
GGGAAAAAGA ACAGTCCACA GGAAGAGGTT GAACTAAAGA AGTTGAAACA TTTGGAGAAG 
TCTGTGGAGA AGATAGCTGA CCAGCTGGAA GAGTTGAATA AAGAGCTTAC TGGAATCCAG 
CAGGGTTTTC TGCCCAAGGA TTTGCAAGCT GAAGCTCTCT GCAAACTTGA TAGGAGAGTA 
AAAGCCACAA TAGAGCAGTT TATGAAGATC TTGGAGGAGA TTGACACACT GATCCTGCCA 
GAAAATTTCA AAGACAGTAG ATTGAAAAGG AAAGGCTTGG TAAAAAAGGT TCAGGCATTC 
CTAGCCGAGT GTGACACAGT GGAGCAGAAC ATCTGCCAGG AGACTGAGCG GCTGCAGTCT 
ACAAACTTTG CCCTGGCCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_001349299.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336228.1 Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu101294
Clone ID Related Accession (Same CDS sequence)	NM_001349299.2 , NM_001349299.1
Accession Version	NM_001349299.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG723775.1, BC001936.1, AK096038.1, AL161445.10 and CN478628.1. Transcript Variant: Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. A minor, shorter isoform (BAG-1, also known as p29), is derived from the third downstream AUG start codon, and is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.3263.1, SRR1803612.224516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9396724) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CTGGGCTCAC CGTGACTGTC ACCCACAGCA ATGAGAAGCA CGACCTTCAT 
GTTACCTCCC AGCAGGGCAG CAGTGAACCA GTTGTCCAAG ACCTGGCCCA GGTTGTTGAA 
GAGGTCATAG GGGTTCCACA GTCTTTTCAG AAACTCATAT TTAAGGGAAA ATCTCTGAAG 
GAAATGGAAA CACCGTTGTC AGCACTTGGA ATACAAGATG GTTGCCGGGT CATGTTAATT 
GGGAAAAAGA ACAGTCCACA GGAAGAGGTT GAACTAAAGA AGTTGAAACA TTTGGAGAAG 
TCTGTGGAGA AGATAGCTGA CCAGCTGGAA GAGTTGAATA AAGAGCTTAC TGGAATCCAG 
CAGGGTTTTC TGCCCAAGGA TTTGCAAGCT GAAGCTCTCT GCAAACTTGA TAGGAGAGTA 
AAAGCCACAA TAGAGCAGTT TATGAAGATC TTGGAGGAGA TTGACACACT GATCCTGCCA 
GAAAATTTCA AAGACAGTAG ATTGAAAAGG AAAGGCTTGG TAAAAAAGGT TCAGGCATTC 
CTAGCCGAGT GTGACACAGT GGAGCAGAAC ATCTGCCAGG AGACTGAGCG GCTGCAGTCT 
ACAAACTTTG CCCTGGCCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336228.1
CDS	502..1125
Translation	MAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_001349299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349299.1

ORF Insert Sequence:

ATGGCGGCAG CTGGGCTCAC CGTGACTGTC ACCCACAGCA ATGAGAAGCA CGACCTTCAT 
GTTACCTCCC AGCAGGGCAG CAGTGAACCA GTTGTCCAAG ACCTGGCCCA GGTTGTTGAA 
GAGGTCATAG GGGTTCCACA GTCTTTTCAG AAACTCATAT TTAAGGGAAA ATCTCTGAAG 
GAAATGGAAA CACCGTTGTC AGCACTTGGA ATACAAGATG GTTGCCGGGT CATGTTAATT 
GGGAAAAAGA ACAGTCCACA GGAAGAGGTT GAACTAAAGA AGTTGAAACA TTTGGAGAAG 
TCTGTGGAGA AGATAGCTGA CCAGCTGGAA GAGTTGAATA AAGAGCTTAC TGGAATCCAG 
CAGGGTTTTC TGCCCAAGGA TTTGCAAGCT GAAGCTCTCT GCAAACTTGA TAGGAGAGTA 
AAAGCCACAA TAGAGCAGTT TATGAAGATC TTGGAGGAGA TTGACACACT GATCCTGCCA 
GAAAATTTCA AAGACAGTAG ATTGAAAAGG AAAGGCTTGG TAAAAAAGGT TCAGGCATTC 
CTAGCCGAGT GTGACACAGT GGAGCAGAAC ATCTGCCAGG AGACTGAGCG GCTGCAGTCT 
ACAAACTTTG CCCTGGCCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_004323.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_004314.6 Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107270
Clone ID Related Accession (Same CDS sequence)	NM_004323.6
Accession Version	NM_004323.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG-1L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356472.17 and AL161445.10. On Nov 23, 2018 this sequence version replaced NM_004323.5. Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. The longest isoform (BAG-1L) is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS ID represents the longest human BAG1 isoform, known as BAG-1L or p50, as described in the literature, including PMIDs 9396724, 9679980, 9747877 and 17662274. This isoform initiates translation at a non-AUG (CUG) start codon that is well-conserved and present in a strong Kozak signal context. Alternative translation initiation at downstream AUG start codons produces three additional isoforms with shorter N-termini, known as BAG-1M or p46, BAG-1S or p36 (also known as p33), and p29. The most abundant of the shorter isoforms, BAG-1S, is represented by CCDS 55301.1. Evidence in PMIDs 9747877 and 17662274 indicates that these isoforms have distinct subcellular distributions, which may contribute to the multifunctionality of the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222749.1, SRR3476690.264741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 9679980, 9396724 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGCTCAGC GCGGGGGGGC GCGGAGACCG CGAGGCGACC GGGAGCGGCT GGGTTCCCGG 
CTGCGCGCCC TTCGGCCAGG CCGGGAGCCG CGCCAGTCGG AGCCCCCGGC CCAGCGTGGT 
CCGCCTCCCT CTGGGCGTCC ACCTGCCCGG AGTACTGCCA GCGGGCATGA CCGACCCACC 
AGGGGCGCCG CCGCCGGCGC TCGCAGGCCG CGGATGAAGA AGAAAACCCG GCGCCGCTCG 
ACCCGGAGCG AGGAGTTGAC CCGGAGCGAG GAGTTGACCC TGAGTGAGGA AGCGACCTGG 
AGTGAAGAGG CGACCCAGAG TGAGGAGGCG ACCCAGGGCG AAGAGATGAA TCGGAGCCAG 
GAGGTGACCC GGGACGAGGA GTCGACCCGG AGCGAGGAGG TGACCAGGGA GGAAATGGCG 
GCAGCTGGGC TCACCGTGAC TGTCACCCAC AGCAATGAGA AGCACGACCT TCATGTTACC 
TCCCAGCAGG GCAGCAGTGA ACCAGTTGTC CAAGACCTGG CCCAGGTTGT TGAAGAGGTC 
ATAGGGGTTC CACAGTCTTT TCAGAAACTC ATATTTAAGG GAAAATCTCT GAAGGAAATG 
GAAACACCGT TGTCAGCACT TGGAATACAA GATGGTTGCC GGGTCATGTT AATTGGGAAA 
AAGAACAGTC CACAGGAAGA GGTTGAACTA AAGAAGTTGA AACATTTGGA GAAGTCTGTG 
GAGAAGATAG CTGACCAGCT GGAAGAGTTG AATAAAGAGC TTACTGGAAT CCAGCAGGGT 
TTTCTGCCCA AGGATTTGCA AGCTGAAGCT CTCTGCAAAC TTGATAGGAG AGTAAAAGCC 
ACAATAGAGC AGTTTATGAA GATCTTGGAG GAGATTGACA CACTGATCCT GCCAGAAAAT 
TTCAAAGACA GTAGATTGAA AAGGAAAGGC TTGGTAAAAA AGGTTCAGGC ATTCCTAGCC 
GAGTGTGACA CAGTGGAGCA GAACATCTGC CAGGAGACTG AGCGGCTGCA GTCTACAAAC 
TTTGCCCTGG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004314.6
CDS	35..1072
Translation	LAQRGGARRPRGDRERLGSRLRALRPGREPRQSEPPAQRGPPPSGRPPARSTASGHDRPTRGAAAGARRPRMKKKTRRRSTRSEELTRSEELTLSEEATWSEEATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_004323.6
Organism	Homo sapiens(human)
Definition	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004323.6

ORF Insert Sequence:

CTGGCTCAGC GCGGGGGGGC GCGGAGACCG CGAGGCGACC GGGAGCGGCT GGGTTCCCGG 
CTGCGCGCCC TTCGGCCAGG CCGGGAGCCG CGCCAGTCGG AGCCCCCGGC CCAGCGTGGT 
CCGCCTCCCT CTGGGCGTCC ACCTGCCCGG AGTACTGCCA GCGGGCATGA CCGACCCACC 
AGGGGCGCCG CCGCCGGCGC TCGCAGGCCG CGGATGAAGA AGAAAACCCG GCGCCGCTCG 
ACCCGGAGCG AGGAGTTGAC CCGGAGCGAG GAGTTGACCC TGAGTGAGGA AGCGACCTGG 
AGTGAAGAGG CGACCCAGAG TGAGGAGGCG ACCCAGGGCG AAGAGATGAA TCGGAGCCAG 
GAGGTGACCC GGGACGAGGA GTCGACCCGG AGCGAGGAGG TGACCAGGGA GGAAATGGCG 
GCAGCTGGGC TCACCGTGAC TGTCACCCAC AGCAATGAGA AGCACGACCT TCATGTTACC 
TCCCAGCAGG GCAGCAGTGA ACCAGTTGTC CAAGACCTGG CCCAGGTTGT TGAAGAGGTC 
ATAGGGGTTC CACAGTCTTT TCAGAAACTC ATATTTAAGG GAAAATCTCT GAAGGAAATG 
GAAACACCGT TGTCAGCACT TGGAATACAA GATGGTTGCC GGGTCATGTT AATTGGGAAA 
AAGAACAGTC CACAGGAAGA GGTTGAACTA AAGAAGTTGA AACATTTGGA GAAGTCTGTG 
GAGAAGATAG CTGACCAGCT GGAAGAGTTG AATAAAGAGC TTACTGGAAT CCAGCAGGGT 
TTTCTGCCCA AGGATTTGCA AGCTGAAGCT CTCTGCAAAC TTGATAGGAG AGTAAAAGCC 
ACAATAGAGC AGTTTATGAA GATCTTGGAG GAGATTGACA CACTGATCCT GCCAGAAAAT 
TTCAAAGACA GTAGATTGAA AAGGAAAGGC TTGGTAAAAA AGGTTCAGGC ATTCCTAGCC 
GAGTGTGACA CAGTGGAGCA GAACATCTGC CAGGAGACTG AGCGGCTGCA GTCTACAAAC 
TTTGCCCTGG CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_001349286.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336215.1 Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu101293
Clone ID Related Accession (Same CDS sequence)	NM_001349286.1 , NM_001349286.2
Accession Version	NM_001349286.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG-1M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG723775.1, BC001936.1, AK096038.1, AL161445.10 and CN478628.1. Transcript Variant: Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. One of the shorter isoforms (BAG-1M, also known as p46), which is derived from the first downstream AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.3263.1, SRR1803612.224516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAGA AAACCCGGCG CCGCTCGACC CGGAGCGAGG AGTTGACCCG GAGCGAGGAG 
TTGACCCTGA GTGAGGAAGC GACCTGGAGT GAAGAGGCGA CCCAGAGTGA GGAGGCGACC 
CAGGGCGAAG AGATGAATCG GAGCCAGGAG GTGACCCGGG ACGAGGAGTC GACCCGGAGC 
GAGGAGGTGA CCAGGGAGGA AATGGCGGCA GCTGGGCTCA CCGTGACTGT CACCCACAGC 
AATGAGAAGC ACGACCTTCA TGTTACCTCC CAGCAGGGCA GCAGTGAACC AGTTGTCCAA 
GACCTGGCCC AGGTTGTTGA AGAGGTCATA GGGGTTCCAC AGTCTTTTCA GAAACTCATA 
TTTAAGGGAA AATCTCTGAA GGAAATGGAA ACACCGTTGT CAGCACTTGG AATACAAGAT 
GGTTGCCGGG TCATGTTAAT TGGGAAAAAG AACAGTCCAC AGGAAGAGGT TGAACTAAAG 
AAGTTGAAAC ATTTGGAGAA GTCTGTGGAG AAGATAGCTG ACCAGCTGGA AGAGTTGAAT 
AAAGAGCTTA CTGGAATCCA GCAGGGTTTT CTGCCCAAGG ATTTGCAAGC TGAAGCTCTC 
TGCAAACTTG ATAGGAGAGT AAAAGCCACA ATAGAGCAGT TTATGAAGAT CTTGGAGGAG 
ATTGACACAC TGATCCTGCC AGAAAATTTC AAAGACAGTA GATTGAAAAG GAAAGGCTTG 
GTAAAAAAGG TTCAGGCATT CCTAGCCGAG TGTGACACAG TGGAGCAGAA CATCTGCCAG 
GAGACTGAGC GGCTGCAGTC TACAAACTTT GCCCTGGCCG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336215.1
CDS	301..1125
Translation	MKKKTRRRSTRSEELTRSEELTLSEEATWSEEATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_001349286.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BCL2 associated athanogene 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349286.1

ORF Insert Sequence:

ATGAAGAAGA AAACCCGGCG CCGCTCGACC CGGAGCGAGG AGTTGACCCG GAGCGAGGAG 
TTGACCCTGA GTGAGGAAGC GACCTGGAGT GAAGAGGCGA CCCAGAGTGA GGAGGCGACC 
CAGGGCGAAG AGATGAATCG GAGCCAGGAG GTGACCCGGG ACGAGGAGTC GACCCGGAGC 
GAGGAGGTGA CCAGGGAGGA AATGGCGGCA GCTGGGCTCA CCGTGACTGT CACCCACAGC 
AATGAGAAGC ACGACCTTCA TGTTACCTCC CAGCAGGGCA GCAGTGAACC AGTTGTCCAA 
GACCTGGCCC AGGTTGTTGA AGAGGTCATA GGGGTTCCAC AGTCTTTTCA GAAACTCATA 
TTTAAGGGAA AATCTCTGAA GGAAATGGAA ACACCGTTGT CAGCACTTGG AATACAAGAT 
GGTTGCCGGG TCATGTTAAT TGGGAAAAAG AACAGTCCAC AGGAAGAGGT TGAACTAAAG 
AAGTTGAAAC ATTTGGAGAA GTCTGTGGAG AAGATAGCTG ACCAGCTGGA AGAGTTGAAT 
AAAGAGCTTA CTGGAATCCA GCAGGGTTTT CTGCCCAAGG ATTTGCAAGC TGAAGCTCTC 
TGCAAACTTG ATAGGAGAGT AAAAGCCACA ATAGAGCAGT TTATGAAGAT CTTGGAGGAG 
ATTGACACAC TGATCCTGCC AGAAAATTTC AAAGACAGTA GATTGAAAAG GAAAGGCTTG 
GTAAAAAAGG TTCAGGCATT CCTAGCCGAG TGTGACACAG TGGAGCAGAA CATCTGCCAG 
GAGACTGAGC GGCTGCAGTC TACAAACTTT GCCCTGGCCG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BAG1 ( NM_001349286.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336215.1 Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu101293
Clone ID Related Accession (Same CDS sequence)	NM_001349286.1 , NM_001349286.2
Accession Version	NM_001349286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	BAG family molecular chaperone regulator 1 isoform BAG-1M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356472.17 and AL161445.10. On Jun 2, 2019 this sequence version replaced NM_001349286.1. Transcript Variant: Transcript Variant: This transcript (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (BAG-1L or p50) is derived from an upstream non-AUG (CUG) start codon, while three shorter isoforms are derived from downstream AUG start codons. One of the shorter isoforms (BAG-1M, also known as p46), which is derived from the first downstream AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222749.1, SRR1660805.3263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAGA AAACCCGGCG CCGCTCGACC CGGAGCGAGG AGTTGACCCG GAGCGAGGAG 
TTGACCCTGA GTGAGGAAGC GACCTGGAGT GAAGAGGCGA CCCAGAGTGA GGAGGCGACC 
CAGGGCGAAG AGATGAATCG GAGCCAGGAG GTGACCCGGG ACGAGGAGTC GACCCGGAGC 
GAGGAGGTGA CCAGGGAGGA AATGGCGGCA GCTGGGCTCA CCGTGACTGT CACCCACAGC 
AATGAGAAGC ACGACCTTCA TGTTACCTCC CAGCAGGGCA GCAGTGAACC AGTTGTCCAA 
GACCTGGCCC AGGTTGTTGA AGAGGTCATA GGGGTTCCAC AGTCTTTTCA GAAACTCATA 
TTTAAGGGAA AATCTCTGAA GGAAATGGAA ACACCGTTGT CAGCACTTGG AATACAAGAT 
GGTTGCCGGG TCATGTTAAT TGGGAAAAAG AACAGTCCAC AGGAAGAGGT TGAACTAAAG 
AAGTTGAAAC ATTTGGAGAA GTCTGTGGAG AAGATAGCTG ACCAGCTGGA AGAGTTGAAT 
AAAGAGCTTA CTGGAATCCA GCAGGGTTTT CTGCCCAAGG ATTTGCAAGC TGAAGCTCTC 
TGCAAACTTG ATAGGAGAGT AAAAGCCACA ATAGAGCAGT TTATGAAGAT CTTGGAGGAG 
ATTGACACAC TGATCCTGCC AGAAAATTTC AAAGACAGTA GATTGAAAAG GAAAGGCTTG 
GTAAAAAAGG TTCAGGCATT CCTAGCCGAG TGTGACACAG TGGAGCAGAA CATCTGCCAG 
GAGACTGAGC GGCTGCAGTC TACAAACTTT GCCCTGGCCG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336215.1
CDS	248..1072
Translation	MKKKTRRRSTRSEELTRSEELTLSEEATWSEEATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE

Target ORF information:

RefSeq Version	NM_001349286.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349286.2

ORF Insert Sequence:

ATGAAGAAGA AAACCCGGCG CCGCTCGACC CGGAGCGAGG AGTTGACCCG GAGCGAGGAG 
TTGACCCTGA GTGAGGAAGC GACCTGGAGT GAAGAGGCGA CCCAGAGTGA GGAGGCGACC 
CAGGGCGAAG AGATGAATCG GAGCCAGGAG GTGACCCGGG ACGAGGAGTC GACCCGGAGC 
GAGGAGGTGA CCAGGGAGGA AATGGCGGCA GCTGGGCTCA CCGTGACTGT CACCCACAGC 
AATGAGAAGC ACGACCTTCA TGTTACCTCC CAGCAGGGCA GCAGTGAACC AGTTGTCCAA 
GACCTGGCCC AGGTTGTTGA AGAGGTCATA GGGGTTCCAC AGTCTTTTCA GAAACTCATA 
TTTAAGGGAA AATCTCTGAA GGAAATGGAA ACACCGTTGT CAGCACTTGG AATACAAGAT 
GGTTGCCGGG TCATGTTAAT TGGGAAAAAG AACAGTCCAC AGGAAGAGGT TGAACTAAAG 
AAGTTGAAAC ATTTGGAGAA GTCTGTGGAG AAGATAGCTG ACCAGCTGGA AGAGTTGAAT 
AAAGAGCTTA CTGGAATCCA GCAGGGTTTT CTGCCCAAGG ATTTGCAAGC TGAAGCTCTC 
TGCAAACTTG ATAGGAGAGT AAAAGCCACA ATAGAGCAGT TTATGAAGAT CTTGGAGGAG 
ATTGACACAC TGATCCTGCC AGAAAATTTC AAAGACAGTA GATTGAAAAG GAAAGGCTTG 
GTAAAAAAGG TTCAGGCATT CCTAGCCGAG TGTGACACAG TGGAGCAGAA CATCTGCCAG 
GAGACTGAGC GGCTGCAGTC TACAAACTTT GCCCTGGCCG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An increase in BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signalling.
European journal of cancer (Oxford, England : 1990)8595-105(2017 11)
Lin PL,Wu TC,Wu DW,Wang L,Chen CY,Lee H

Over-expression of BAG-1 in head and neck squamous cell carcinomas (HNSCC) is associated with cisplatin-resistance.
Journal of translational medicine15(1)189(2017 Sep)
Liu S,Ren B,Gao H,Liao S,Zhai YX,Li S,Su XJ,Jin P,Stroncek D,Xu Z,Zeng Q,Li Y

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BAG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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