PTCH1 cDNA ORF clone, Homo sapiens(human)

The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17265 NM_001083604.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 NM_001083605.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 NM_001083606.3
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 NM_001083607.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu18800 NM_000264.5
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu17265 XM_011518869.1
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 XM_011518872.1
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 XM_011518871.1
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 XM_005252102.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 XM_011518870.1
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu102968 NM_001354919.1
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu59996 NM_001354918.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu17397 NM_001083603.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu19644 NM_001083602.2
Latest version!
Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
View Old Accession Versions >>
OHu19644 NM_001083602.1 Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu17397 NM_001083603.1 Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu17265 NM_001083606.1 Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 NM_001083604.1 Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu59996 NM_001354918.1 Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu17265 NM_001083606.2 Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17265 NM_001083605.1 Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu18800 NM_000264.3 Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu17265 NM_001083607.1 Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu18800 NM_000264.4 Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083605.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform S
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189439.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1c') differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077074.1
    CDS343..4233
    Translation

    Target ORF information:

    RefSeq Version NM_001083605.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083605.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083604.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189438.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083604.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077073.1
    CDS360..4250
    Translation

    Target ORF information:

    RefSeq Version NM_001083604.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083606.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U43148.1, AI358880.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083606.1. Transcript Variant: This variant (1d) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43148.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077075.1
    CDS442..4332
    Translation

    Target ORF information:

    RefSeq Version NM_001083606.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083606.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083606.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform S
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U43148.1, AI358880.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1d) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43148.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077075.1
    CDS442..4332
    Translation

    Target ORF information:

    RefSeq Version NM_001083606.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083604.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform S
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189438.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1c) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077073.1
    CDS360..4250
    Translation

    Target ORF information:

    RefSeq Version NM_001083604.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083607.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform S
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189440.1, BF195352.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1e) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077076.1
    CDS516..4406
    Translation

    Target ORF information:

    RefSeq Version NM_001083607.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083607.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083606.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161729.27, U43148.1, AI358880.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Jun 2, 2019 this sequence version replaced NM_001083606.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43148.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077075.1
    CDS452..4342
    Translation

    Target ORF information:

    RefSeq Version NM_001083606.3
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083606.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083605.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189439.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083605.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077074.1
    CDS343..4233
    Translation

    Target ORF information:

    RefSeq Version NM_001083605.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083605.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version NM_001083607.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-25
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189440.1, BF195352.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083607.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077076.1
    CDS516..4406
    Translation

    Target ORF information:

    RefSeq Version NM_001083607.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083607.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18800
    Clone ID Related Accession (Same CDS sequence) NM_000264.4 , NM_000264.3 , NM_000264.5
    Accession Version NM_000264.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-10
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI494442.1, U59464.1, DB093644.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_000264.3. Transcript Variant: This variant (1b) encodes the longest isoform (L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U59464.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG
    GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA
    GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT
    CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC
    GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC
    CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC
    ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA
    GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA
    TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC
    AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC
    CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC
    ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG
    CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG
    AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC
    CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT
    GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG
    CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT
    GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC
    TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC
    GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT
    CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC
    AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA
    GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC
    ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC
    ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG
    CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC
    TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC
    TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG
    TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC
    CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG
    GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC
    GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT
    GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG
    CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG
    GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC
    ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA
    TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA
    CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC
    TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC
    TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA
    AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG
    AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG
    CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA
    GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC
    CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC
    GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC
    GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC
    TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT
    GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG
    CCCCGGGGAA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000255.2
    CDS189..4532
    Translation

    Target ORF information:

    RefSeq Version NM_000264.4
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000264.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG
    GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA
    GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT
    CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC
    GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC
    CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC
    ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA
    GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA
    TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC
    AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC
    CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC
    ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG
    CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG
    AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC
    CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT
    GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG
    CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT
    GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC
    TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC
    GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT
    CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC
    AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA
    GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC
    ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC
    ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG
    CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC
    TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC
    TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG
    TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC
    CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG
    GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC
    GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT
    GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG
    CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG
    GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC
    ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA
    TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA
    CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC
    TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC
    TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA
    AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG
    AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG
    CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA
    GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC
    CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC
    GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC
    GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC
    TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT
    GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG
    CCCCGGGGAA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18800
    Clone ID Related Accession (Same CDS sequence) NM_000264.4 , NM_000264.3 , NM_000264.5
    Accession Version NM_000264.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform L
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI494442.1, U59464.1, DB093644.1, AB209495.1, BX117041.1 and CR744004.1. This sequence is a reference standard in the RefSeqGene project. On Mar 29, 2007 this sequence version replaced NM_000264.2. Transcript Variant: This variant (1b) encodes the longest isoform (L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U59464.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG
    GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA
    GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT
    CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC
    GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC
    CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC
    ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA
    GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA
    TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC
    AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC
    CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC
    ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG
    CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG
    AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC
    CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT
    GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG
    CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT
    GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC
    TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC
    GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT
    CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC
    AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA
    GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC
    ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC
    ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG
    CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC
    TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC
    TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG
    TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC
    CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG
    GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC
    GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT
    GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG
    CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG
    GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC
    ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA
    TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA
    CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC
    TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC
    TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA
    AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG
    AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG
    CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA
    GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC
    CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC
    GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC
    GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC
    TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT
    GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG
    CCCCGGGGAA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000255.2
    CDS189..4532
    Translation

    Target ORF information:

    RefSeq Version NM_000264.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000264.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG
    GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA
    GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT
    CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC
    GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC
    CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC
    ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA
    GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA
    TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC
    AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC
    CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC
    ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG
    CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG
    AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC
    CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT
    GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG
    CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT
    GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC
    TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC
    GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT
    CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC
    AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA
    GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC
    ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC
    ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG
    CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC
    TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC
    TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG
    TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC
    CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG
    GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC
    GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT
    GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG
    CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG
    GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC
    ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA
    TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA
    CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC
    TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC
    TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA
    AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG
    AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG
    CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA
    GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC
    CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC
    GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC
    GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC
    TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT
    GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG
    CCCCGGGGAA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18800
    Clone ID Related Accession (Same CDS sequence) NM_000264.4 , NM_000264.3 , NM_000264.5
    Accession Version NM_000264.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-25
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161729.27. On Jul 26, 2019 this sequence version replaced NM_000264.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U59464.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG
    GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA
    GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT
    CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC
    GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC
    CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC
    ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA
    GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA
    TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC
    AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC
    CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC
    ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG
    CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG
    AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC
    CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT
    GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG
    CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT
    GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC
    TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC
    GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT
    CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC
    AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA
    GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC
    ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC
    ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG
    CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC
    TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC
    TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG
    TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC
    CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG
    GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC
    GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT
    GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG
    CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG
    GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC
    ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA
    TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA
    CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC
    TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC
    TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA
    AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG
    AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG
    CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA
    GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC
    CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC
    GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC
    GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC
    TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT
    GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG
    CCCCGGGGAA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000255.2
    CDS906..5249
    Translation

    Target ORF information:

    RefSeq Version NM_000264.5
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000264.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG
    GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA
    GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT
    CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC
    GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC
    CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC
    ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA
    GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA
    TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC
    AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC
    CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC
    ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG
    CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG
    AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC
    CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT
    GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG
    CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT
    GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC
    TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC
    GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT
    CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC
    AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA
    GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC
    CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC
    ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC
    ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG
    CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC
    TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC
    TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG
    TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC
    CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG
    GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC
    GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT
    GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG
    CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG
    GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC
    ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA
    TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA
    CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC
    TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC
    TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA
    AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG
    AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG
    CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA
    GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC
    CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC
    GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC
    GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC
    TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT
    GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG
    CCCCGGGGAA GCAGCTCCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version XM_005252102.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005252159.1
    Translation

    Target ORF information:

    RefSeq Version XM_005252102.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005252102.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version XM_011518872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517174.1
    Translation

    Target ORF information:

    RefSeq Version XM_011518872.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version XM_011518871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517173.1
    Translation

    Target ORF information:

    RefSeq Version XM_011518871.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version XM_011518869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517171.1
    Translation

    Target ORF information:

    RefSeq Version XM_011518869.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17265
    Clone ID Related Accession (Same CDS sequence) XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
    Accession Version XM_011518870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517172.1
    Translation

    Target ORF information:

    RefSeq Version XM_011518870.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011518870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
    ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC
    ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA
    GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT
    TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA
    CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA
    AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC
    ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA
    AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC
    AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT
    GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA
    ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA
    GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC
    GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG
    AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC
    TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG
    GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC
    GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT
    GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC
    CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG
    TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG
    GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT
    TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC
    TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC
    GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC
    CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC
    ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC
    GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG
    CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA
    CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA
    GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG
    AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT
    GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC
    TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT
    GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG
    CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC
    AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC
    AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT
    GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC
    GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC
    AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT
    GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT
    GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC
    GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC
    ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG
    ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG
    GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC
    ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG
    AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG
    TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT
    TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT
    CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC
    CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC
    GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG
    TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC
    CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT
    CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG
    TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG
    TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT
    GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC
    CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG
    ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG
    AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC
    CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG
    CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102968
    Clone ID Related Accession (Same CDS sequence) NM_001354919.1
    Accession Version NM_001354919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-20
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC043542.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC
    CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC
    CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA
    GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT
    TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA
    TACCTCCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341848.1
    CDS587..1138
    Translation

    Target ORF information:

    RefSeq Version NM_001354919.1
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC
    CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC
    CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA
    GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT
    TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA
    TACCTCCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59996
    Clone ID Related Accession (Same CDS sequence) NM_001354918.1 , NM_001354918.2
    Accession Version NM_001354918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161729.27. On Jul 26, 2019 this sequence version replaced NM_001354918.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: KY652975.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG
    GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT
    TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC
    ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG
    TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT
    CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC
    TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC
    ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT
    GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG
    CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG
    ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC
    TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC
    TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG
    GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA
    GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT
    GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC
    CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC
    ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT
    CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT
    TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC
    CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC
    ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC
    GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA
    GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC
    CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA
    AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC
    TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC
    AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG
    GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC
    CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA
    GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC
    CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC
    ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC
    TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC
    GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC
    CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC
    CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA
    GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC
    CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC
    GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC
    CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG
    CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC
    CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA
    GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG
    GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC
    CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC
    CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG
    CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341847.1
    CDS906..5093
    Translation

    Target ORF information:

    RefSeq Version NM_001354918.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG
    GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT
    TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC
    ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG
    TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT
    CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC
    TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC
    ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT
    GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG
    CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG
    ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC
    TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC
    TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG
    GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA
    GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT
    GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC
    CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC
    ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT
    CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT
    TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC
    CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC
    ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC
    GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA
    GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC
    CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA
    AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC
    TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC
    AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG
    GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC
    CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA
    GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC
    CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC
    ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC
    TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC
    GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC
    CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC
    CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA
    GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC
    CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC
    GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC
    CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG
    CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC
    CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA
    GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG
    GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC
    CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC
    CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG
    CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17397
    Clone ID Related Accession (Same CDS sequence) NM_001083603.2 , NM_001083603.1
    Accession Version NM_001083603.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-25
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform L'
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189437.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083603.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
    GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA
    GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA
    GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG
    AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC
    CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG
    GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA
    TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA
    CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT
    TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT
    CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG
    ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT
    GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG
    ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC
    TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC
    GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC
    TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG
    GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC
    TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG
    AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG
    GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG
    GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT
    CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA
    GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC
    CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT
    GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC
    CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC
    TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT
    CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG
    GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA
    CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC
    CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC
    CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG
    TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC
    TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC
    CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG
    AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG
    CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT
    ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC
    GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG
    CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG
    CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT
    TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG
    GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC
    CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT
    CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC
    CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC
    GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC
    GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC
    CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG
    CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC
    GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT
    CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG
    CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG
    CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC
    CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG
    AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC
    CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077072.1
    CDS146..4486
    Translation

    Target ORF information:

    RefSeq Version NM_001083603.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083603.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
    GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA
    GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA
    GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG
    AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC
    CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG
    GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA
    TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA
    CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT
    TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT
    CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG
    ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT
    GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG
    ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC
    TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC
    GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC
    TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG
    GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC
    TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG
    AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG
    GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG
    GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT
    CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA
    GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC
    CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT
    GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC
    CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC
    TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT
    CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG
    GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA
    CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC
    CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC
    CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG
    TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC
    TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC
    CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG
    AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG
    CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT
    ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC
    GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG
    CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG
    CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT
    TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG
    GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC
    CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT
    CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC
    CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC
    GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC
    GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC
    CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG
    CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC
    GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT
    CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG
    CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG
    CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC
    CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG
    AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC
    CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17397
    Clone ID Related Accession (Same CDS sequence) NM_001083603.2 , NM_001083603.1
    Accession Version NM_001083603.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform L'
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189437.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1a') differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences result in a protein (isoform L') with a shorter N-terminus and distinct translation initiation site, compared to isoform L. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
    GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA
    GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA
    GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG
    AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC
    CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG
    GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA
    TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA
    CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT
    TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT
    CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG
    ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT
    GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG
    ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC
    TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC
    GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC
    TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG
    GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC
    TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG
    AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG
    GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG
    GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT
    CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA
    GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC
    CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT
    GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC
    CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC
    TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT
    CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG
    GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA
    CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC
    CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC
    CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG
    TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC
    TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC
    CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG
    AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG
    CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT
    ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC
    GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG
    CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG
    CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT
    TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG
    GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC
    CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT
    CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC
    CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC
    GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC
    GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC
    CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG
    CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC
    GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT
    CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG
    CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG
    CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC
    CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG
    AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC
    CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077072.1
    CDS146..4486
    Translation

    Target ORF information:

    RefSeq Version NM_001083603.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
    GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA
    GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA
    GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG
    AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC
    TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC
    GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT
    TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC
    CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG
    GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT
    TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA
    TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA
    CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT
    TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG
    CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT
    CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT
    GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG
    ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG
    ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT
    GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG
    ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC
    TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC
    GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC
    TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG
    GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG
    GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC
    TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG
    AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG
    GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG
    GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT
    CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA
    GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC
    CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT
    GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG
    CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC
    CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC
    TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT
    CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG
    GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA
    CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC
    AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC
    CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC
    AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT
    GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC
    CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG
    TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC
    TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC
    CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG
    AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG
    CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT
    ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC
    ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC
    GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG
    CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG
    CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT
    TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC
    ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG
    GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC
    CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT
    CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC
    CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC
    GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC
    GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC
    CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC
    CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG
    CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC
    GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT
    CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG
    CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG
    CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC
    CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG
    AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC
    CGGGGAAGCA GCTCCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19644
    Clone ID Related Accession (Same CDS sequence) NM_001083602.2 , NM_001083602.1
    Accession Version NM_001083602.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-25
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC043542.1, AB189436.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083602.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC
    CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC
    CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA
    GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT
    TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA
    TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA
    GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG
    GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC
    ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA
    TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA
    GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC
    TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG
    GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC
    CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC
    TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC
    CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC
    GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA
    CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC
    AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA
    ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG
    AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA
    CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC
    AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG
    GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT
    GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC
    TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA
    CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA
    GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC
    AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC
    TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG
    AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC
    CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT
    CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG
    GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA
    GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA
    GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC
    TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG
    TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC
    TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC
    CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG
    CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG
    ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG
    GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC
    CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC
    TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT
    GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA
    GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC
    TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT
    TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC
    GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC
    TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC
    CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC
    CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT
    CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC
    CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG
    GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077071.1
    CDS587..4732
    Translation

    Target ORF information:

    RefSeq Version NM_001083602.2
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083602.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC
    CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC
    CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA
    GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT
    TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA
    TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA
    GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG
    GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC
    ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA
    TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA
    GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC
    TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG
    GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC
    CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC
    TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC
    CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC
    GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA
    CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC
    AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA
    ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG
    AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA
    CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC
    AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG
    GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT
    GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC
    TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA
    CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA
    GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC
    AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC
    TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG
    AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC
    CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT
    CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG
    GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA
    GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA
    GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC
    TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG
    TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC
    TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC
    CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG
    CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG
    ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG
    GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC
    CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC
    TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT
    GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA
    GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC
    TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT
    TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC
    GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC
    TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC
    CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC
    CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT
    CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC
    CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG
    GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19644
    Clone ID Related Accession (Same CDS sequence) NM_001083602.2 , NM_001083602.1
    Accession Version NM_001083602.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-24
    Organism Homo sapiens(Human)
    Product protein patched homolog 1 isoform M
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189436.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1a) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences result in a protein (isoform M) with a shorter N-terminus and distinct translation initiation site, compared to isoform L. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC
    CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC
    CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA
    GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT
    TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA
    TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA
    GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG
    GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC
    ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA
    TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA
    GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC
    TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG
    GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC
    CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC
    TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC
    CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC
    GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA
    CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC
    AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA
    ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG
    AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA
    CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC
    AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG
    GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT
    GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC
    TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA
    CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA
    GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC
    AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC
    TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG
    AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC
    CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT
    CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG
    GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA
    GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA
    GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC
    TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG
    TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC
    TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC
    CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG
    CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG
    ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG
    GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC
    CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC
    TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT
    GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA
    GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC
    TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT
    TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC
    GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC
    TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC
    CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC
    CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT
    CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC
    CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG
    GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077071.1
    CDS495..4640
    Translation

    Target ORF information:

    RefSeq Version NM_001083602.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
    TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC
    CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG
    GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG
    ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT
    GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC
    CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA
    GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT
    TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA
    TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA
    GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG
    GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC
    ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA
    TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA
    GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA
    ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC
    TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG
    GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC
    CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC
    TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG
    GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC
    CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC
    GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA
    CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC
    AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA
    ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC
    ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG
    AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA
    CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC
    AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG
    GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT
    GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC
    TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC
    TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA
    CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT
    GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA
    GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC
    ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC
    AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC
    TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG
    AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC
    CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT
    CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG
    GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA
    GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA
    GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC
    TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG
    TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC
    TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC
    CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG
    CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG
    ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG
    GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC
    CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC
    TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT
    GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA
    GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC
    TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT
    TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC
    GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC
    TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC
    CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC
    CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT
    ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT
    CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG
    CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC
    CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC
    GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG
    GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59996
    Clone ID Related Accession (Same CDS sequence) NM_001354918.1 , NM_001354918.2
    Accession Version NM_001354918.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-10
    Organism Homo sapiens(human)
    Product protein patched homolog 1 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI494319.1, KY652975.1, AB209495.1, AK124593.1, CR744004.1 and AI417736.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: KY652975.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG
    GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT
    TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC
    ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG
    TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT
    CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC
    TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC
    ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT
    GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG
    CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG
    ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC
    TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC
    TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG
    GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA
    GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT
    GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC
    CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC
    ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT
    CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT
    TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC
    CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC
    ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC
    GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA
    GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC
    CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA
    AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC
    TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC
    AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG
    GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC
    CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA
    GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC
    CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC
    ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC
    TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC
    GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC
    CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC
    CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA
    GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC
    CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC
    GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC
    CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG
    CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC
    CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA
    GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG
    GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC
    CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC
    CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG
    CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341847.1
    CDS189..4376
    Translation

    Target ORF information:

    RefSeq Version NM_001354918.1
    Organism Homo sapiens(human)
    Definition Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001354918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
    ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC
    CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC
    GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA
    GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG
    TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC
    CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA
    AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG
    ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC
    TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA
    CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA
    GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA
    TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC
    CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA
    ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC
    GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC
    CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG
    TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG
    CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG
    TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG
    AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT
    TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC
    CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG
    GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT
    TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC
    ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG
    TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT
    CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC
    TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC
    ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT
    GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG
    CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG
    ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC
    TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC
    TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG
    GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA
    GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT
    GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC
    CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC
    ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT
    CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT
    TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC
    CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC
    ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC
    GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA
    GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC
    CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA
    AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC
    TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC
    AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG
    GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC
    CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA
    GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC
    CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC
    ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC
    TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC
    GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC
    CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC
    CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA
    GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC
    CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC
    GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC
    CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG
    CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC
    CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA
    GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG
    GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC
    CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC
    CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG
    CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.