NHSL1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017011086.1	XP_016866575.1	NHS-like protein 1 isoform X1
XM_011535974.2	XP_011534276.1	NHS-like protein 1 isoform X11
XM_011535967.3	XP_011534269.1	NHS-like protein 1 isoform X1
XM_011535971.2	XP_011534273.1	NHS-like protein 1 isoform X8
XM_011535973.3	XP_011534275.1	NHS-like protein 1 isoform X10
XM_017011089.1	XP_016866578.1	NHS-like protein 1 isoform X12
XM_011535976.1	XP_011534278.1	NHS-like protein 1 isoform X13
XM_005267062.4	XP_005267119.1	NHS-like protein 1 isoform X2
XM_017011086.2	XP_016866575.1	NHS-like protein 1 isoform X1
XM_024446497.1	XP_024302265.1	NHS-like protein 1 isoform X14
XM_011535966.2	XP_011534268.1	NHS-like protein 1 isoform X1
XM_011535973.2	XP_011534275.1	NHS-like protein 1 isoform X10
XM_011535970.2	XP_011534272.1	NHS-like protein 1 isoform X6
XM_011535972.2	XP_011534274.1	NHS-like protein 1 isoform X9
XM_011535974.3	XP_011534276.1	NHS-like protein 1 isoform X11
NM_020464.1	NP_065197.1	NHS-like protein 1 isoform 1
NM_001144060.1	NP_001137532.1	NHS-like protein 1 isoform 2
NM_020464.2	NP_065197.1	NHS-like protein 1 isoform 1
NM_001144060.2	NP_001137532.1	NHS-like protein 1 isoform 2
XM_011535966.3	XP_011534268.1	NHS-like protein 1 isoform X1
XM_017011087.1	XP_016866576.1	NHS-like protein 1 isoform X3
XM_011535968.3	XP_011534270.1	NHS-like protein 1 isoform X4
XM_011535967.2	XP_011534269.1	NHS-like protein 1 isoform X1
XM_011535965.2	XP_011534267.1	NHS-like protein 1 isoform X1
XM_011535968.2	XP_011534270.1	NHS-like protein 1 isoform X4
XM_005267062.5	XP_005267119.1	NHS-like protein 1 isoform X2
XM_011535969.2	XP_011534271.1	NHS-like protein 1 isoform X5
XM_017011088.1	XP_016866577.1	NHS-like protein 1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15179	NM_020464.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu105823	XM_024446497.1 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu96699	XM_017011087.1 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu96700	XM_017011088.1 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu96701	XM_017011089.1 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu15007	NM_001144060.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu40882	XM_005267062.5 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70398	XM_011535966.3 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70398	XM_011535965.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70398	XM_011535967.3 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70398	XM_017011086.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70399	XM_011535968.3 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70400	XM_011535969.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70401	XM_011535970.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70402	XM_011535971.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70403	XM_011535972.2 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70404	XM_011535973.3 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70405	XM_011535974.3 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70407	XM_011535976.1 Latest version!	Homo sapiens NHS like 1 (NHSL1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu70398	XM_017011086.1	Homo sapiens NHS like 1 (NHSL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70404	XM_011535973.2	Homo sapiens NHS like 1 (NHSL1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70405	XM_011535974.2	Homo sapiens NHS like 1 (NHSL1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu15179	NM_020464.1	Homo sapiens NHS like 1 (NHSL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu15007	NM_001144060.1	Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu70398	XM_011535966.2	Homo sapiens NHS like 1 (NHSL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70398	XM_011535967.2	Homo sapiens NHS like 1 (NHSL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu70399	XM_011535968.2	Homo sapiens NHS like 1 (NHSL1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu40882	XM_005267062.4	Homo sapiens NHS like 1 (NHSL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

NHSL1 ( NM_020464.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_065197.1 Homo sapiens NHS like 1 (NHSL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15179
Clone ID Related Accession (Same CDS sequence)	NM_020464.1 , NM_020464.2
Accession Version	NM_020464.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591375.8 and AL391669.11. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGCATC AGGCACTGGC CAAGATGATG CTGATGGCCA CTCAGTGTAC 
ACCCCTGATC ACTACTCTAC ACTAGGAAGG TTCAATAGCT GTCGGTCTGC TGGGCAGCGC 
TCAGAAACCA GGGACTCCAG CTGTCAGACG GAGGATGTGA AGGTCGTACC ACCTTCCATG 
AGGAGAATCA GGGCACAGAA GGGGCAAGGC ATTGCTGCCC AGATGGGCCA CTTCTCAGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAT TCTGCAGGGA TCGTATTCCC TTCCCGCCTT 
GACAGTGATG CTGGCTTCCA TAGTCTTCCG CGTTCTGGAG CAAGGGCAAA CATTCAGTCC 
CTTGAGCCGA GGCTGGGTGC CCTCGGCCCT GCAGGAGACA TGAATGGCAC TTTCCTCTAC 
CAGAGAGGTC ACCCACAAGC AGATGAAAAC TTAGGCCATT TAGGAGGTGC CTCAGGGACT 
GGAACACTTT TGAGACCCAA ATCCCAGGAG TTGAGACACT TCGAGAGTGA AAATATAATG 
AGCCCAGCGT GTGTGGTTTC TCCTCATGCA ACCTACTCCA CCAGCATCAT CCCAAATGCC 
ACACTGTCTT CCTCTTCCGA GGTCATCGCT ATTCCCACTG CTCAGAGTGC GGGACAGCGG 
GAAAGTAAAA GTTCTGGCTC ATCACATGCA AGGATAAAAT CCAGAGACCA CCTCATCTCC 
AGGCATGCTG TGAAAGGTGA TCCTCAGTCT CCCGGTCGCC ACTGGAATGA GGGCCATGCC 
ACCATTCTTT CACAGGACTT AGACCCTCAT TCCCCTGGTG AACCCGCACT GTTGTCCCTC 
TGTGACTCAG CCGTCCCTCT AAATGCTCCA GCAAATAGGG AGAATGGGTC CCAAGCTATG 
CCGTATAATT GTAGAAACAA CCTGGCCTTC CCAGCCCACC CCCAAGATGT GGATGGCAAG 
AGTGAATCTA GTTATTCAGG GGGCGGAGGG CACAGCAGCT CGGAGCCCTG GGAATACAAA 
TCCTCAGGTA ATGGAAGGGC ATCCCCCCTG AAGCCGCATT TAGCAACTCC TGGCTATTCC 
ACTCCCACAA GTAACATGAG CAGCTGCAGT TTGGACCAAA CGTCCAACAA AGAGGATGCT 
GGGTCGCTGT ATTCTGAGGA CCACGATGGC TACTGTGCAT CTGTGCACAC TGACTCTGGA 
CATGGATCTG GGAATCTGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGCGTG 
ATCAATGTTT TTGTTGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCCAA TTACCAGGAT 
AAATCCCTAT CAAGAAACAT CTCTTTGAAG AAAGCAAAGA AGCCTCCCCT GCCACCCTCC 
CGGACAGACT CCCTCCGCAG GATTCCCAAG AAGAGCAGCC AGTGCAACGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCAC ACTCCAGCAC TCGCTGCAGC TGAGCCTCCC AGGCAAGAGT 
GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GACTTGGAAG AGCCCTGGCT GCCCCGCTCC 
CGGAGCCAGA GCACAGTTAG TGCTGGCAGC AGCATGACTT CCGCCACCAC CCCCAATGTC 
TACTCCCTGT GCGGGGCCAC GCCATCGCAG AGTGACACAA GCAGCGTCAA GTCAGAGTAC 
ACGGACCCCT GGGGTTATTA CATTGACTAC ACGGGCATGC AGGAAGATCC GGGGAACCCG 
GCAGGGGGCT GTTCAACCAG CAGTGGGGTG CCCACTGGGA ACGGGCCAGT CCGCCATGTC 
CAAGAAGGGT CCAGAGCCAC AATGCCCCAA GTGCCCGGTG GTTCAGTCAA ACCAAAGATC 
ATGTCACCAG AGAAGTCACA CAGAGTCATT TCTCCATCCA GTGGGTATTC CAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAGTGTC ACCAGCAAAC 
GGGAAGGGGA AGCCCAAGCC CAAGGTACCA GAAAGGAAGT CCTCTCTGAT ATCTTCAGTA 
TCCATTTCCT CATCGTCCAC TTCTCTTTCT TCTAGTACTT CTACTGAAGG AAGTGGCACT 
ATGAAGAAGC TGGATCCAGC CGTGGGCTCT CCCCCGGCTC CTCCTCCTCC TCCTGTTCCC 
TCTCCTCCAT TCCCTTGTCC TGCAGACAGG TCTCCTTTCC TTCCTCCCCC ACCTCCTGTC 
ACAGATTGCT CCCAGGGCTC TCCTCTGCCT CACTCTCCTG TGTTCCCCCC TCCGCCGCCA 
GAAGCTCTCA TTCCTTTCTG CTCCCCACCT GATTGGTGCC TTTCTCCTCC CCGCCCTGCA 
CTGAGCCCCA TTCTTCCAGA TTCACCTGTG TCCTTGCCAT TGCCCCCACC TCTCTTACCT 
TCCTCGGAAC CCCCACCTGC CCCACCTCTT GACCCCAAAT TCATGAAAGA CACCAGGCCG 
CCTTTCACAA ATTCTGGCCA GCCAGAATCC TCCCGGGGAT CCTTGAGGCC GCCTTCTACC 
AAGGAGGAGA CCAGCAGGCC CCCCATGCCC CTGATAACCA CGGAAGCATT GCAGATGGTG 
CAGTTGAGGC CCGTGAGAAA GAACTCAGGC GCTGAGGCGG CACAGTTGTC TGAACGAACA 
GCTCAGGAAC AACGAACTCC AGTTGCTCCA CAGTACCACT TAAAGCCATC TGCTTTCCTG 
AAATCCCGAA ATAGCACAAA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC TGTGACAAGC 
TCGCTTCCGA CGCCTGCCAA GAGCTCGAGT CAGGGTGACC ATGGCAGTGC GGCTGAGCGT 
GGTGGCCCTG TGAGCCGCAG CCCTGGAGCT CCAAGCGCTG GGGAGGCAGA GGCTCGGCCC 
AGCCCCAGCA CCACCCCACT CCCAGACTCT TCACCCAGCA GGAAGCCACC CCCCATTTCC 
AAGAAGCCCA AACTGTTCCT GGTGGTACCA CCTCCGCAGA AAGATTTTGC AGTGGAGCCC 
GCAGAGAACG TGAGCGAAGC CCTCCGAGCT GTGCCCAGCC CCACGACGGG AGAGGAGGGC 
TCTGTGCACA GCAGGGAGGC AAAAGAGAGT TCTGCAGCCC AAGCTGGCTC TCATGCCACG 
CACCCTGGCA CCTCGGTTCT TGAGGGAGGA GCTGCAGGAT CCATGTCTCC CAGCAGAGTG 
GAAGCCAATG TCCCCATGGT TCAGCCTGAT GTCTCACCAG CCCCCAAGCA GGAGGAGCCA 
GCCGAGAACA GTGCGGATAC TGGGGGCGAT GGGGAGAGCT GCCTATCTCA ACAGGACGGA 
GCAGCTGGGG TGCCGGAGAC CAACGCAGCC GGTTCATCCT CAGAGGCCTG TGACTTCCTC 
AAGGAAGACG GGAATGATGA GGTAATGACC CCCAGTCGAC CCAGGACCAC AGAAGACCTT 
TTTGCAGCTA TTCACAGATC CAAAAGGAAA GTCCTCGGCC GTAGAGATTC AGATGATGAC 
CACTCCCGAA ACCATTCTCC CTCCCCGCCC GTGACACCCA CCGGCGCTGC CCCAAGCCTG 
GCCTCTCCAA AGCAAGTGGG GTCGATTCAG AGAAGCATCC GAAAGAGCAG CACCAGCAGT 
GACAACTTCA AAGCTCTGCT GCTGAAAAAG GGCAGTCGTT CAGACACCAG CGCCCGCATG 
TCTGCAGCAG AGATGCTCAA GAACACAGAC CCTAGATTCC AGAGGTCGAG GTCAGAGCCT 
TCCCCAGATG CCCCCGAGAG CCCGTCAAGC TGCTCCCCAA GCAAGAACAG AAGGGCGCAG 
GAGGAGTGGG CCAAGAACGA AGGCTTGATG CCTCGGAGTC TGTCCTTTTC CGGCCCCAGG 
TACGGCCGCA GCCGAACGCC GCCTTCTGCC GCCAGCAGCA GGTACAGCAT GCGGAACCGG 
ATCCAGAGCA GCCCCATGAC CGTCATCTCG GAGGGAGAAG GGGAAGCCGT GGAGCCTGTG 
GACAGCATAG CCCGCGGGGC TCTGGGCGCT GCGGAGGGAT GTTCCCTGGA CGGACTGGCG 
AGGGAGGAGA TGGACGAGGG CGGCCTGCTC TGTGGGGAGG GGCCTGCCGC CTCCCTGCAG 
CCCCAGGCCC CCGGCCCTGT GGATGGGACA GCCAGTGCAG AGGGCAGAGA GCCCTCCCCA 
CAGTGTGGCG GTTCTCTGAG CGAGGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065197.1
CDS	621..5453
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	NM_020464.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020464.1

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGCATC AGGCACTGGC CAAGATGATG CTGATGGCCA CTCAGTGTAC 
ACCCCTGATC ACTACTCTAC ACTAGGAAGG TTCAATAGCT GTCGGTCTGC TGGGCAGCGC 
TCAGAAACCA GGGACTCCAG CTGTCAGACG GAGGATGTGA AGGTCGTACC ACCTTCCATG 
AGGAGAATCA GGGCACAGAA GGGGCAAGGC ATTGCTGCCC AGATGGGCCA CTTCTCAGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAT TCTGCAGGGA TCGTATTCCC TTCCCGCCTT 
GACAGTGATG CTGGCTTCCA TAGTCTTCCG CGTTCTGGAG CAAGGGCAAA CATTCAGTCC 
CTTGAGCCGA GGCTGGGTGC CCTCGGCCCT GCAGGAGACA TGAATGGCAC TTTCCTCTAC 
CAGAGAGGTC ACCCACAAGC AGATGAAAAC TTAGGCCATT TAGGAGGTGC CTCAGGGACT 
GGAACACTTT TGAGACCCAA ATCCCAGGAG TTGAGACACT TCGAGAGTGA AAATATAATG 
AGCCCAGCGT GTGTGGTTTC TCCTCATGCA ACCTACTCCA CCAGCATCAT CCCAAATGCC 
ACACTGTCTT CCTCTTCCGA GGTCATCGCT ATTCCCACTG CTCAGAGTGC GGGACAGCGG 
GAAAGTAAAA GTTCTGGCTC ATCACATGCA AGGATAAAAT CCAGAGACCA CCTCATCTCC 
AGGCATGCTG TGAAAGGTGA TCCTCAGTCT CCCGGTCGCC ACTGGAATGA GGGCCATGCC 
ACCATTCTTT CACAGGACTT AGACCCTCAT TCCCCTGGTG AACCCGCACT GTTGTCCCTC 
TGTGACTCAG CCGTCCCTCT AAATGCTCCA GCAAATAGGG AGAATGGGTC CCAAGCTATG 
CCGTATAATT GTAGAAACAA CCTGGCCTTC CCAGCCCACC CCCAAGATGT GGATGGCAAG 
AGTGAATCTA GTTATTCAGG GGGCGGAGGG CACAGCAGCT CGGAGCCCTG GGAATACAAA 
TCCTCAGGTA ATGGAAGGGC ATCCCCCCTG AAGCCGCATT TAGCAACTCC TGGCTATTCC 
ACTCCCACAA GTAACATGAG CAGCTGCAGT TTGGACCAAA CGTCCAACAA AGAGGATGCT 
GGGTCGCTGT ATTCTGAGGA CCACGATGGC TACTGTGCAT CTGTGCACAC TGACTCTGGA 
CATGGATCTG GGAATCTGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGCGTG 
ATCAATGTTT TTGTTGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCCAA TTACCAGGAT 
AAATCCCTAT CAAGAAACAT CTCTTTGAAG AAAGCAAAGA AGCCTCCCCT GCCACCCTCC 
CGGACAGACT CCCTCCGCAG GATTCCCAAG AAGAGCAGCC AGTGCAACGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCAC ACTCCAGCAC TCGCTGCAGC TGAGCCTCCC AGGCAAGAGT 
GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GACTTGGAAG AGCCCTGGCT GCCCCGCTCC 
CGGAGCCAGA GCACAGTTAG TGCTGGCAGC AGCATGACTT CCGCCACCAC CCCCAATGTC 
TACTCCCTGT GCGGGGCCAC GCCATCGCAG AGTGACACAA GCAGCGTCAA GTCAGAGTAC 
ACGGACCCCT GGGGTTATTA CATTGACTAC ACGGGCATGC AGGAAGATCC GGGGAACCCG 
GCAGGGGGCT GTTCAACCAG CAGTGGGGTG CCCACTGGGA ACGGGCCAGT CCGCCATGTC 
CAAGAAGGGT CCAGAGCCAC AATGCCCCAA GTGCCCGGTG GTTCAGTCAA ACCAAAGATC 
ATGTCACCAG AGAAGTCACA CAGAGTCATT TCTCCATCCA GTGGGTATTC CAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAGTGTC ACCAGCAAAC 
GGGAAGGGGA AGCCCAAGCC CAAGGTACCA GAAAGGAAGT CCTCTCTGAT ATCTTCAGTA 
TCCATTTCCT CATCGTCCAC TTCTCTTTCT TCTAGTACTT CTACTGAAGG AAGTGGCACT 
ATGAAGAAGC TGGATCCAGC CGTGGGCTCT CCCCCGGCTC CTCCTCCTCC TCCTGTTCCC 
TCTCCTCCAT TCCCTTGTCC TGCAGACAGG TCTCCTTTCC TTCCTCCCCC ACCTCCTGTC 
ACAGATTGCT CCCAGGGCTC TCCTCTGCCT CACTCTCCTG TGTTCCCCCC TCCGCCGCCA 
GAAGCTCTCA TTCCTTTCTG CTCCCCACCT GATTGGTGCC TTTCTCCTCC CCGCCCTGCA 
CTGAGCCCCA TTCTTCCAGA TTCACCTGTG TCCTTGCCAT TGCCCCCACC TCTCTTACCT 
TCCTCGGAAC CCCCACCTGC CCCACCTCTT GACCCCAAAT TCATGAAAGA CACCAGGCCG 
CCTTTCACAA ATTCTGGCCA GCCAGAATCC TCCCGGGGAT CCTTGAGGCC GCCTTCTACC 
AAGGAGGAGA CCAGCAGGCC CCCCATGCCC CTGATAACCA CGGAAGCATT GCAGATGGTG 
CAGTTGAGGC CCGTGAGAAA GAACTCAGGC GCTGAGGCGG CACAGTTGTC TGAACGAACA 
GCTCAGGAAC AACGAACTCC AGTTGCTCCA CAGTACCACT TAAAGCCATC TGCTTTCCTG 
AAATCCCGAA ATAGCACAAA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC TGTGACAAGC 
TCGCTTCCGA CGCCTGCCAA GAGCTCGAGT CAGGGTGACC ATGGCAGTGC GGCTGAGCGT 
GGTGGCCCTG TGAGCCGCAG CCCTGGAGCT CCAAGCGCTG GGGAGGCAGA GGCTCGGCCC 
AGCCCCAGCA CCACCCCACT CCCAGACTCT TCACCCAGCA GGAAGCCACC CCCCATTTCC 
AAGAAGCCCA AACTGTTCCT GGTGGTACCA CCTCCGCAGA AAGATTTTGC AGTGGAGCCC 
GCAGAGAACG TGAGCGAAGC CCTCCGAGCT GTGCCCAGCC CCACGACGGG AGAGGAGGGC 
TCTGTGCACA GCAGGGAGGC AAAAGAGAGT TCTGCAGCCC AAGCTGGCTC TCATGCCACG 
CACCCTGGCA CCTCGGTTCT TGAGGGAGGA GCTGCAGGAT CCATGTCTCC CAGCAGAGTG 
GAAGCCAATG TCCCCATGGT TCAGCCTGAT GTCTCACCAG CCCCCAAGCA GGAGGAGCCA 
GCCGAGAACA GTGCGGATAC TGGGGGCGAT GGGGAGAGCT GCCTATCTCA ACAGGACGGA 
GCAGCTGGGG TGCCGGAGAC CAACGCAGCC GGTTCATCCT CAGAGGCCTG TGACTTCCTC 
AAGGAAGACG GGAATGATGA GGTAATGACC CCCAGTCGAC CCAGGACCAC AGAAGACCTT 
TTTGCAGCTA TTCACAGATC CAAAAGGAAA GTCCTCGGCC GTAGAGATTC AGATGATGAC 
CACTCCCGAA ACCATTCTCC CTCCCCGCCC GTGACACCCA CCGGCGCTGC CCCAAGCCTG 
GCCTCTCCAA AGCAAGTGGG GTCGATTCAG AGAAGCATCC GAAAGAGCAG CACCAGCAGT 
GACAACTTCA AAGCTCTGCT GCTGAAAAAG GGCAGTCGTT CAGACACCAG CGCCCGCATG 
TCTGCAGCAG AGATGCTCAA GAACACAGAC CCTAGATTCC AGAGGTCGAG GTCAGAGCCT 
TCCCCAGATG CCCCCGAGAG CCCGTCAAGC TGCTCCCCAA GCAAGAACAG AAGGGCGCAG 
GAGGAGTGGG CCAAGAACGA AGGCTTGATG CCTCGGAGTC TGTCCTTTTC CGGCCCCAGG 
TACGGCCGCA GCCGAACGCC GCCTTCTGCC GCCAGCAGCA GGTACAGCAT GCGGAACCGG 
ATCCAGAGCA GCCCCATGAC CGTCATCTCG GAGGGAGAAG GGGAAGCCGT GGAGCCTGTG 
GACAGCATAG CCCGCGGGGC TCTGGGCGCT GCGGAGGGAT GTTCCCTGGA CGGACTGGCG 
AGGGAGGAGA TGGACGAGGG CGGCCTGCTC TGTGGGGAGG GGCCTGCCGC CTCCCTGCAG 
CCCCAGGCCC CCGGCCCTGT GGATGGGACA GCCAGTGCAG AGGGCAGAGA GCCCTCCCCA 
CAGTGTGGCG GTTCTCTGAG CGAGGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( NM_020464.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065197.1 Homo sapiens NHS like 1 (NHSL1), transcript variant 1, mRNA.

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CloneID	OHu15179
Clone ID Related Accession (Same CDS sequence)	NM_020464.1 , NM_020464.2
Accession Version	NM_020464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591375.8 and AL391669.11. On Jun 2, 2019 this sequence version replaced NM_020464.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGCATC AGGCACTGGC CAAGATGATG CTGATGGCCA CTCAGTGTAC 
ACCCCTGATC ACTACTCTAC ACTAGGAAGG TTCAATAGCT GTCGGTCTGC TGGGCAGCGC 
TCAGAAACCA GGGACTCCAG CTGTCAGACG GAGGATGTGA AGGTCGTACC ACCTTCCATG 
AGGAGAATCA GGGCACAGAA GGGGCAAGGC ATTGCTGCCC AGATGGGCCA CTTCTCAGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAT TCTGCAGGGA TCGTATTCCC TTCCCGCCTT 
GACAGTGATG CTGGCTTCCA TAGTCTTCCG CGTTCTGGAG CAAGGGCAAA CATTCAGTCC 
CTTGAGCCGA GGCTGGGTGC CCTCGGCCCT GCAGGAGACA TGAATGGCAC TTTCCTCTAC 
CAGAGAGGTC ACCCACAAGC AGATGAAAAC TTAGGCCATT TAGGAGGTGC CTCAGGGACT 
GGAACACTTT TGAGACCCAA ATCCCAGGAG TTGAGACACT TCGAGAGTGA AAATATAATG 
AGCCCAGCGT GTGTGGTTTC TCCTCATGCA ACCTACTCCA CCAGCATCAT CCCAAATGCC 
ACACTGTCTT CCTCTTCCGA GGTCATCGCT ATTCCCACTG CTCAGAGTGC GGGACAGCGG 
GAAAGTAAAA GTTCTGGCTC ATCACATGCA AGGATAAAAT CCAGAGACCA CCTCATCTCC 
AGGCATGCTG TGAAAGGTGA TCCTCAGTCT CCCGGTCGCC ACTGGAATGA GGGCCATGCC 
ACCATTCTTT CACAGGACTT AGACCCTCAT TCCCCTGGTG AACCCGCACT GTTGTCCCTC 
TGTGACTCAG CCGTCCCTCT AAATGCTCCA GCAAATAGGG AGAATGGGTC CCAAGCTATG 
CCGTATAATT GTAGAAACAA CCTGGCCTTC CCAGCCCACC CCCAAGATGT GGATGGCAAG 
AGTGAATCTA GTTATTCAGG GGGCGGAGGG CACAGCAGCT CGGAGCCCTG GGAATACAAA 
TCCTCAGGTA ATGGAAGGGC ATCCCCCCTG AAGCCGCATT TAGCAACTCC TGGCTATTCC 
ACTCCCACAA GTAACATGAG CAGCTGCAGT TTGGACCAAA CGTCCAACAA AGAGGATGCT 
GGGTCGCTGT ATTCTGAGGA CCACGATGGC TACTGTGCAT CTGTGCACAC TGACTCTGGA 
CATGGATCTG GGAATCTGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGCGTG 
ATCAATGTTT TTGTTGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCCAA TTACCAGGAT 
AAATCCCTAT CAAGAAACAT CTCTTTGAAG AAAGCAAAGA AGCCTCCCCT GCCACCCTCC 
CGGACAGACT CCCTCCGCAG GATTCCCAAG AAGAGCAGCC AGTGCAACGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCAC ACTCCAGCAC TCGCTGCAGC TGAGCCTCCC AGGCAAGAGT 
GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GACTTGGAAG AGCCCTGGCT GCCCCGCTCC 
CGGAGCCAGA GCACAGTTAG TGCTGGCAGC AGCATGACTT CCGCCACCAC CCCCAATGTC 
TACTCCCTGT GCGGGGCCAC GCCATCGCAG AGTGACACAA GCAGCGTCAA GTCAGAGTAC 
ACGGACCCCT GGGGTTATTA CATTGACTAC ACGGGCATGC AGGAAGATCC GGGGAACCCG 
GCAGGGGGCT GTTCAACCAG CAGTGGGGTG CCCACTGGGA ACGGGCCAGT CCGCCATGTC 
CAAGAAGGGT CCAGAGCCAC AATGCCCCAA GTGCCCGGTG GTTCAGTCAA ACCAAAGATC 
ATGTCACCAG AGAAGTCACA CAGAGTCATT TCTCCATCCA GTGGGTATTC CAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAGTGTC ACCAGCAAAC 
GGGAAGGGGA AGCCCAAGCC CAAGGTACCA GAAAGGAAGT CCTCTCTGAT ATCTTCAGTA 
TCCATTTCCT CATCGTCCAC TTCTCTTTCT TCTAGTACTT CTACTGAAGG AAGTGGCACT 
ATGAAGAAGC TGGATCCAGC CGTGGGCTCT CCCCCGGCTC CTCCTCCTCC TCCTGTTCCC 
TCTCCTCCAT TCCCTTGTCC TGCAGACAGG TCTCCTTTCC TTCCTCCCCC ACCTCCTGTC 
ACAGATTGCT CCCAGGGCTC TCCTCTGCCT CACTCTCCTG TGTTCCCCCC TCCGCCGCCA 
GAAGCTCTCA TTCCTTTCTG CTCCCCACCT GATTGGTGCC TTTCTCCTCC CCGCCCTGCA 
CTGAGCCCCA TTCTTCCAGA TTCACCTGTG TCCTTGCCAT TGCCCCCACC TCTCTTACCT 
TCCTCGGAAC CCCCACCTGC CCCACCTCTT GACCCCAAAT TCATGAAAGA CACCAGGCCG 
CCTTTCACAA ATTCTGGCCA GCCAGAATCC TCCCGGGGAT CCTTGAGGCC GCCTTCTACC 
AAGGAGGAGA CCAGCAGGCC CCCCATGCCC CTGATAACCA CGGAAGCATT GCAGATGGTG 
CAGTTGAGGC CCGTGAGAAA GAACTCAGGC GCTGAGGCGG CACAGTTGTC TGAACGAACA 
GCTCAGGAAC AACGAACTCC AGTTGCTCCA CAGTACCACT TAAAGCCATC TGCTTTCCTG 
AAATCCCGAA ATAGCACAAA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC TGTGACAAGC 
TCGCTTCCGA CGCCTGCCAA GAGCTCGAGT CAGGGTGACC ATGGCAGTGC GGCTGAGCGT 
GGTGGCCCTG TGAGCCGCAG CCCTGGAGCT CCAAGCGCTG GGGAGGCAGA GGCTCGGCCC 
AGCCCCAGCA CCACCCCACT CCCAGACTCT TCACCCAGCA GGAAGCCACC CCCCATTTCC 
AAGAAGCCCA AACTGTTCCT GGTGGTACCA CCTCCGCAGA AAGATTTTGC AGTGGAGCCC 
GCAGAGAACG TGAGCGAAGC CCTCCGAGCT GTGCCCAGCC CCACGACGGG AGAGGAGGGC 
TCTGTGCACA GCAGGGAGGC AAAAGAGAGT TCTGCAGCCC AAGCTGGCTC TCATGCCACG 
CACCCTGGCA CCTCGGTTCT TGAGGGAGGA GCTGCAGGAT CCATGTCTCC CAGCAGAGTG 
GAAGCCAATG TCCCCATGGT TCAGCCTGAT GTCTCACCAG CCCCCAAGCA GGAGGAGCCA 
GCCGAGAACA GTGCGGATAC TGGGGGCGAT GGGGAGAGCT GCCTATCTCA ACAGGACGGA 
GCAGCTGGGG TGCCGGAGAC CAACGCAGCC GGTTCATCCT CAGAGGCCTG TGACTTCCTC 
AAGGAAGACG GGAATGATGA GGTAATGACC CCCAGTCGAC CCAGGACCAC AGAAGACCTT 
TTTGCAGCTA TTCACAGATC CAAAAGGAAA GTCCTCGGCC GTAGAGATTC AGATGATGAC 
CACTCCCGAA ACCATTCTCC CTCCCCGCCC GTGACACCCA CCGGCGCTGC CCCAAGCCTG 
GCCTCTCCAA AGCAAGTGGG GTCGATTCAG AGAAGCATCC GAAAGAGCAG CACCAGCAGT 
GACAACTTCA AAGCTCTGCT GCTGAAAAAG GGCAGTCGTT CAGACACCAG CGCCCGCATG 
TCTGCAGCAG AGATGCTCAA GAACACAGAC CCTAGATTCC AGAGGTCGAG GTCAGAGCCT 
TCCCCAGATG CCCCCGAGAG CCCGTCAAGC TGCTCCCCAA GCAAGAACAG AAGGGCGCAG 
GAGGAGTGGG CCAAGAACGA AGGCTTGATG CCTCGGAGTC TGTCCTTTTC CGGCCCCAGG 
TACGGCCGCA GCCGAACGCC GCCTTCTGCC GCCAGCAGCA GGTACAGCAT GCGGAACCGG 
ATCCAGAGCA GCCCCATGAC CGTCATCTCG GAGGGAGAAG GGGAAGCCGT GGAGCCTGTG 
GACAGCATAG CCCGCGGGGC TCTGGGCGCT GCGGAGGGAT GTTCCCTGGA CGGACTGGCG 
AGGGAGGAGA TGGACGAGGG CGGCCTGCTC TGTGGGGAGG GGCCTGCCGC CTCCCTGCAG 
CCCCAGGCCC CCGGCCCTGT GGATGGGACA GCCAGTGCAG AGGGCAGAGA GCCCTCCCCA 
CAGTGTGGCG GTTCTCTGAG CGAGGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065197.1
CDS	659..5491
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	NM_020464.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020464.2

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGCATC AGGCACTGGC CAAGATGATG CTGATGGCCA CTCAGTGTAC 
ACCCCTGATC ACTACTCTAC ACTAGGAAGG TTCAATAGCT GTCGGTCTGC TGGGCAGCGC 
TCAGAAACCA GGGACTCCAG CTGTCAGACG GAGGATGTGA AGGTCGTACC ACCTTCCATG 
AGGAGAATCA GGGCACAGAA GGGGCAAGGC ATTGCTGCCC AGATGGGCCA CTTCTCAGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAT TCTGCAGGGA TCGTATTCCC TTCCCGCCTT 
GACAGTGATG CTGGCTTCCA TAGTCTTCCG CGTTCTGGAG CAAGGGCAAA CATTCAGTCC 
CTTGAGCCGA GGCTGGGTGC CCTCGGCCCT GCAGGAGACA TGAATGGCAC TTTCCTCTAC 
CAGAGAGGTC ACCCACAAGC AGATGAAAAC TTAGGCCATT TAGGAGGTGC CTCAGGGACT 
GGAACACTTT TGAGACCCAA ATCCCAGGAG TTGAGACACT TCGAGAGTGA AAATATAATG 
AGCCCAGCGT GTGTGGTTTC TCCTCATGCA ACCTACTCCA CCAGCATCAT CCCAAATGCC 
ACACTGTCTT CCTCTTCCGA GGTCATCGCT ATTCCCACTG CTCAGAGTGC GGGACAGCGG 
GAAAGTAAAA GTTCTGGCTC ATCACATGCA AGGATAAAAT CCAGAGACCA CCTCATCTCC 
AGGCATGCTG TGAAAGGTGA TCCTCAGTCT CCCGGTCGCC ACTGGAATGA GGGCCATGCC 
ACCATTCTTT CACAGGACTT AGACCCTCAT TCCCCTGGTG AACCCGCACT GTTGTCCCTC 
TGTGACTCAG CCGTCCCTCT AAATGCTCCA GCAAATAGGG AGAATGGGTC CCAAGCTATG 
CCGTATAATT GTAGAAACAA CCTGGCCTTC CCAGCCCACC CCCAAGATGT GGATGGCAAG 
AGTGAATCTA GTTATTCAGG GGGCGGAGGG CACAGCAGCT CGGAGCCCTG GGAATACAAA 
TCCTCAGGTA ATGGAAGGGC ATCCCCCCTG AAGCCGCATT TAGCAACTCC TGGCTATTCC 
ACTCCCACAA GTAACATGAG CAGCTGCAGT TTGGACCAAA CGTCCAACAA AGAGGATGCT 
GGGTCGCTGT ATTCTGAGGA CCACGATGGC TACTGTGCAT CTGTGCACAC TGACTCTGGA 
CATGGATCTG GGAATCTGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGCGTG 
ATCAATGTTT TTGTTGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCCAA TTACCAGGAT 
AAATCCCTAT CAAGAAACAT CTCTTTGAAG AAAGCAAAGA AGCCTCCCCT GCCACCCTCC 
CGGACAGACT CCCTCCGCAG GATTCCCAAG AAGAGCAGCC AGTGCAACGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCAC ACTCCAGCAC TCGCTGCAGC TGAGCCTCCC AGGCAAGAGT 
GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GACTTGGAAG AGCCCTGGCT GCCCCGCTCC 
CGGAGCCAGA GCACAGTTAG TGCTGGCAGC AGCATGACTT CCGCCACCAC CCCCAATGTC 
TACTCCCTGT GCGGGGCCAC GCCATCGCAG AGTGACACAA GCAGCGTCAA GTCAGAGTAC 
ACGGACCCCT GGGGTTATTA CATTGACTAC ACGGGCATGC AGGAAGATCC GGGGAACCCG 
GCAGGGGGCT GTTCAACCAG CAGTGGGGTG CCCACTGGGA ACGGGCCAGT CCGCCATGTC 
CAAGAAGGGT CCAGAGCCAC AATGCCCCAA GTGCCCGGTG GTTCAGTCAA ACCAAAGATC 
ATGTCACCAG AGAAGTCACA CAGAGTCATT TCTCCATCCA GTGGGTATTC CAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAGTGTC ACCAGCAAAC 
GGGAAGGGGA AGCCCAAGCC CAAGGTACCA GAAAGGAAGT CCTCTCTGAT ATCTTCAGTA 
TCCATTTCCT CATCGTCCAC TTCTCTTTCT TCTAGTACTT CTACTGAAGG AAGTGGCACT 
ATGAAGAAGC TGGATCCAGC CGTGGGCTCT CCCCCGGCTC CTCCTCCTCC TCCTGTTCCC 
TCTCCTCCAT TCCCTTGTCC TGCAGACAGG TCTCCTTTCC TTCCTCCCCC ACCTCCTGTC 
ACAGATTGCT CCCAGGGCTC TCCTCTGCCT CACTCTCCTG TGTTCCCCCC TCCGCCGCCA 
GAAGCTCTCA TTCCTTTCTG CTCCCCACCT GATTGGTGCC TTTCTCCTCC CCGCCCTGCA 
CTGAGCCCCA TTCTTCCAGA TTCACCTGTG TCCTTGCCAT TGCCCCCACC TCTCTTACCT 
TCCTCGGAAC CCCCACCTGC CCCACCTCTT GACCCCAAAT TCATGAAAGA CACCAGGCCG 
CCTTTCACAA ATTCTGGCCA GCCAGAATCC TCCCGGGGAT CCTTGAGGCC GCCTTCTACC 
AAGGAGGAGA CCAGCAGGCC CCCCATGCCC CTGATAACCA CGGAAGCATT GCAGATGGTG 
CAGTTGAGGC CCGTGAGAAA GAACTCAGGC GCTGAGGCGG CACAGTTGTC TGAACGAACA 
GCTCAGGAAC AACGAACTCC AGTTGCTCCA CAGTACCACT TAAAGCCATC TGCTTTCCTG 
AAATCCCGAA ATAGCACAAA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC TGTGACAAGC 
TCGCTTCCGA CGCCTGCCAA GAGCTCGAGT CAGGGTGACC ATGGCAGTGC GGCTGAGCGT 
GGTGGCCCTG TGAGCCGCAG CCCTGGAGCT CCAAGCGCTG GGGAGGCAGA GGCTCGGCCC 
AGCCCCAGCA CCACCCCACT CCCAGACTCT TCACCCAGCA GGAAGCCACC CCCCATTTCC 
AAGAAGCCCA AACTGTTCCT GGTGGTACCA CCTCCGCAGA AAGATTTTGC AGTGGAGCCC 
GCAGAGAACG TGAGCGAAGC CCTCCGAGCT GTGCCCAGCC CCACGACGGG AGAGGAGGGC 
TCTGTGCACA GCAGGGAGGC AAAAGAGAGT TCTGCAGCCC AAGCTGGCTC TCATGCCACG 
CACCCTGGCA CCTCGGTTCT TGAGGGAGGA GCTGCAGGAT CCATGTCTCC CAGCAGAGTG 
GAAGCCAATG TCCCCATGGT TCAGCCTGAT GTCTCACCAG CCCCCAAGCA GGAGGAGCCA 
GCCGAGAACA GTGCGGATAC TGGGGGCGAT GGGGAGAGCT GCCTATCTCA ACAGGACGGA 
GCAGCTGGGG TGCCGGAGAC CAACGCAGCC GGTTCATCCT CAGAGGCCTG TGACTTCCTC 
AAGGAAGACG GGAATGATGA GGTAATGACC CCCAGTCGAC CCAGGACCAC AGAAGACCTT 
TTTGCAGCTA TTCACAGATC CAAAAGGAAA GTCCTCGGCC GTAGAGATTC AGATGATGAC 
CACTCCCGAA ACCATTCTCC CTCCCCGCCC GTGACACCCA CCGGCGCTGC CCCAAGCCTG 
GCCTCTCCAA AGCAAGTGGG GTCGATTCAG AGAAGCATCC GAAAGAGCAG CACCAGCAGT 
GACAACTTCA AAGCTCTGCT GCTGAAAAAG GGCAGTCGTT CAGACACCAG CGCCCGCATG 
TCTGCAGCAG AGATGCTCAA GAACACAGAC CCTAGATTCC AGAGGTCGAG GTCAGAGCCT 
TCCCCAGATG CCCCCGAGAG CCCGTCAAGC TGCTCCCCAA GCAAGAACAG AAGGGCGCAG 
GAGGAGTGGG CCAAGAACGA AGGCTTGATG CCTCGGAGTC TGTCCTTTTC CGGCCCCAGG 
TACGGCCGCA GCCGAACGCC GCCTTCTGCC GCCAGCAGCA GGTACAGCAT GCGGAACCGG 
ATCCAGAGCA GCCCCATGAC CGTCATCTCG GAGGGAGAAG GGGAAGCCGT GGAGCCTGTG 
GACAGCATAG CCCGCGGGGC TCTGGGCGCT GCGGAGGGAT GTTCCCTGGA CGGACTGGCG 
AGGGAGGAGA TGGACGAGGG CGGCCTGCTC TGTGGGGAGG GGCCTGCCGC CTCCCTGCAG 
CCCCAGGCCC CCGGCCCTGT GGATGGGACA GCCAGTGCAG AGGGCAGAGA GCCCTCCCCA 
CAGTGTGGCG GTTCTCTGAG CGAGGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_024446497.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302265.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu105823
Clone ID Related Accession (Same CDS sequence)	XM_024446497.1
Accession Version	XM_024446497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302265.1
CDS	1174..5517
Translation	MRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_024446497.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446497.1

ORF Insert Sequence:

ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_017011087.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866576.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X5, mRNA.

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CloneID	OHu96699
Clone ID Related Accession (Same CDS sequence)	XM_017011087.1
Accession Version	XM_017011087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATCA CATTCTTCCC CATGCCAAAA GAAACCTTGC TGGAGTCTTT CAGGGACCTG 
TGCTTCCCCC TGCTTTGGAA AGCAAAACTA CTGAAACCGG TTTCCAACCT AGATGAGGAA 
AGCCGATGGA CAGTCCACTA CACTGCCCCG TGGCACCAGC AGGAGAATGT GTTCCTTCCC 
ACCACAAGAC CCCCCTGTGT TGAGGACCTG CACCGCCAAG CCAAGCTCAA CCTCAAATCA 
GTACTGAGGG TGATTCCAGA AGATGAGATG GGAGACCAGA ATTCTGGTTG CAGTGTTGTC 
AGTGAATGCG ATAAGTTGCG GCATGATGGC TACCGCAGTT CTCAGTACTA CTCTCAGGGA 
CCCACCTTTG CGGCCAACGC CAGCCCATTC TGTGATGATT ACCAAGATGA AGATGAAGAA 
ACAGATCAAA AGTGTTCTCT GTCTTCATCA GAAGAAGAAA GATTTATTTC CATCAGGAGA 
CCTAAAACAC CAGCCTCAAG TGACTTCTCC GACCTTAATA CTCAGACGAA CTGGACCAAG 
TCGCTTCCAC TGCCAACACC AGAAGAGAAG ATGCGACAGC AAGCCCAAAC AGTCCAGGCT 
GACGTGGTGC CTATTAACAT AACTGGGGAG AATTTCGATC GCCAGGCCAG CCTTCGGCGG 
TCTCTAATTT ACACAGACAC TCTGGTAAGA CGACCGAAGA AAGTCAAAAG GAGAAAGACT 
ATTACAGGAG TCCCTGACAA CATACAGAAG GAGCTAGCAT CAGGCACTGG CCAAGATGAT 
GCTGATGGCC ACTCAGTGTA CACCCCTGAT CACTACTCTA CACTAGGAAG GTTCAATAGC 
TGTCGGTCTG CTGGGCAGCG CTCAGAAACC AGGGACTCCA GCTGTCAGAC GGAGGATGTG 
AAGGTCGTAC CACCTTCCAT GAGGAGAATC AGGGCACAGA AGGGGCAAGG CATTGCTGCC 
CAGATGGGCC ACTTCTCAGG TTCCTCTGGC AACATGTCTG TGCTGAGCGA TTCTGCAGGG 
ATCGTATTCC CTTCCCGCCT TGACAGTGAT GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA 
GCAAGGGCAA ACATTCAGTC CCTTGAGCCG AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC 
ATGAATGGCA CTTTCCTCTA CCAGAGAGGT CACCCACAAG CAGATGAAAA CTTAGGCCAT 
TTAGGAGGTG CCTCAGGGAC TGGAACACTT TTGAGACCCA AATCCCAGGA GTTGAGACAC 
TTCGAGAGTG AAAATATAAT GAGCCCAGCG TGTGTGGTTT CTCCTCATGC AACCTACTCC 
ACCAGCATCA TCCCAAATGC CACACTGTCT TCCTCTTCCG AGGTCATCGC TATTCCCACT 
GCTCAGAGTG CGGGACAGCG GGAAAGTAAA AGTTCTGGCT CATCACATGC AAGGATAAAA 
TCCAGAGACC ACCTCATCTC CAGGCATGCT GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC 
CACTGGAATG AGGGCCATGC CACCATTCTT TCACAGGACT TAGACCCTCA TTCCCCTGGT 
GAACCCGCAC TGTTGTCCCT CTGTGACTCA GCCGTCCCTC TAAATGCTCC AGCAAATAGG 
GAGAATGGGT CCCAAGCTAT GCCGTATAAT TGTAGAAACA ACCTGGCCTT CCCAGCCCAC 
CCCCAAGATG TGGATGGCAA GAGTGAATCT AGTTATTCAG GGGGCGGAGG GCACAGCAGC 
TCGGAGCCCT GGGAATACAA ATCCTCAGGT AATGGAAGGG CATCCCCCCT GAAGCCGCAT 
TTAGCAACTC CTGGCTATTC CACTCCCACA AGTAACATGA GCAGCTGCAG TTTGGACCAA 
ACGTCCAACA AAGAGGATGC TGGGTCGCTG TATTCTGAGG ACCACGATGG CTACTGTGCA 
TCTGTGCACA CTGACTCTGG ACATGGATCT GGGAATCTGT GCAATAGCAG TGATGGCTTT 
GGGAACCCCA GGCACAGCGT GATCAATGTT TTTGTTGGAA GAGCTCAGAA AAACCAAGGG 
GACCGGTCCA ATTACCAGGA TAAATCCCTA TCAAGAAACA TCTCTTTGAA GAAAGCAAAG 
AAGCCTCCCC TGCCACCCTC CCGGACAGAC TCCCTCCGCA GGATTCCCAA GAAGAGCAGC 
CAGTGCAACG GGCAGGTGCT CAACGAGAGC CTGATCGCCA CACTCCAGCA CTCGCTGCAG 
CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG CCCTCCCAGA GCCCCTGCAG TGACTTGGAA 
GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG AGCACAGTTA GTGCTGGCAG CAGCATGACT 
TCCGCCACCA CCCCCAATGT CTACTCCCTG TGCGGGGCCA CGCCATCGCA GAGTGACACA 
AGCAGCGTCA AGTCAGAGTA CACGGACCCC TGGGGTTATT ACATTGACTA CACGGGCATG 
CAGGAAGATC CGGGGAACCC GGCAGGGGGC TGTTCAACCA GCAGTGGGGT GCCCACTGGG 
AACGGGCCAG TCCGCCATGT CCAAGAAGGG TCCAGAGCCA CAATGCCCCA AGTGCCCGGT 
GGTTCAGTCA AACCAAAGAT CATGTCACCA GAGAAGTCAC ACAGAGTCAT TTCTCCATCC 
AGTGGGTATT CCAGCCAGTC GAATACACCC ACAGCACTCA CCCCTGTGCC TGTGTTTTTA 
AAATCAGTGT CACCAGCAAA CGGGAAGGGG AAGCCCAAGC CCAAGGTACC AGAAAGGAAG 
TCCTCTCTGA TATCTTCAGT ATCCATTTCC TCATCGTCCA CTTCTCTTTC TTCTAGTACT 
TCTACTGAAG GAAGTGGCAC TATGAAGAAG CTGGATCCAG CCGTGGGCTC TCCCCCGGCT 
CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA TTCCCTTGTC CTGCAGACAG GTCTCCTTTC 
CTTCCTCCCC CACCTCCTGT CACAGATTGC TCCCAGGGCT CTCCTCTGCC TCACTCTCCT 
GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC ATTCCTTTCT GCTCCCCACC TGATTGGTGC 
CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC ATTCTTCCAG ATTCACCTGT GTCCTTGCCA 
TTGCCCCCAC CTCTCTTACC TTCCTCGGAA CCCCCACCTG CCCCACCTCT TGACCCCAAA 
TTCATGAAAG ACACCAGGCC GCCTTTCACA AATTCTGGCC AGCCAGAATC CTCCCGGGGA 
TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG ACCAGCAGGC CCCCCATGCC CCTGATAACC 
ACGGAAGCAT TGCAGATGGT GCAGTTGAGG CCCGTGAGAA AGAACTCAGG CGCTGAGGCG 
GCACAGTTGT CTGAACGAAC AGCTCAGGAA CAACGAACTC CAGTTGCTCC ACAGTACCAC 
TTAAAGCCAT CTGCTTTCCT GAAATCCCGA AATAGCACAA ATGAAATGGA GAGTGAAAGC 
CAGCCTGCCT CTGTGACAAG CTCGCTTCCG ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC 
CATGGCAGTG CGGCTGAGCG TGGTGGCCCT GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT 
GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC ACCACCCCAC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATTTC CAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AAAGATTTTG CAGTGGAGCC CGCAGAGAAC GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC 
CCCACGACGG GAGAGGAGGG CTCTGTGCAC AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC 
CAAGCTGGCT CTCATGCCAC GCACCCTGGC ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA 
TCCATGTCTC CCAGCAGAGT GGAAGCCAAT GTCCCCATGG TTCAGCCTGA TGTCTCACCA 
GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC AGTGCGGATA CTGGGGGCGA TGGGGAGAGC 
TGCCTATCTC AACAGGACGG AGCAGCTGGG GTGCCGGAGA CCAACGCAGC CGGTTCATCC 
TCAGAGGCCT GTGACTTCCT CAAGGAAGAC GGGAATGATG AGGTAATGAC CCCCAGTCGA 
CCCAGGACCA CAGAAGACCT TTTTGCAGCT ATTCACAGAT CCAAAAGGAA AGTCCTCGGC 
CGTAGAGATT CAGATGATGA CCACTCCCGA AACCATTCTC CCTCCCCGCC CGTGACACCC 
ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA AAGCAAGTGG GGTCGATTCA GAGAAGCATC 
CGAAAGAGCA GCACCAGCAG TGACAACTTC AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT 
TCAGACACCA GCGCCCGCAT GTCTGCAGCA GAGATGCTCA AGAACACAGA CCCTAGATTC 
CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA 
AGCAAGAACA GAAGGGCGCA GGAGGAGTGG GCCAAGAACG AAGGCTTGAT GCCTCGGAGT 
CTGTCCTTTT CCGGCCCCAG GTACGGCCGC AGCCGAACGC CGCCTTCTGC CGCCAGCAGC 
AGGTACAGCA TGCGGAACCG GATCCAGAGC AGCCCCATGA CCGTCATCTC GGAGGGAGAA 
GGGGAAGCCG TGGAGCCTGT GGACAGCATA GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA 
TGTTCCCTGG ACGGACTGGC GAGGGAGGAG ATGGACGAGG GCGGCCTGCT CTGTGGGGAG 
GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC CCCGGCCCTG TGGATGGGAC AGCCAGTGCA 
GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866576.1
CDS	998..5911
Translation	MFITFFPMPKETLLESFRDLCFPLLWKAKLLKPVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_017011087.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011087.1

ORF Insert Sequence:

ATGTTTATCA CATTCTTCCC CATGCCAAAA GAAACCTTGC TGGAGTCTTT CAGGGACCTG 
TGCTTCCCCC TGCTTTGGAA AGCAAAACTA CTGAAACCGG TTTCCAACCT AGATGAGGAA 
AGCCGATGGA CAGTCCACTA CACTGCCCCG TGGCACCAGC AGGAGAATGT GTTCCTTCCC 
ACCACAAGAC CCCCCTGTGT TGAGGACCTG CACCGCCAAG CCAAGCTCAA CCTCAAATCA 
GTACTGAGGG TGATTCCAGA AGATGAGATG GGAGACCAGA ATTCTGGTTG CAGTGTTGTC 
AGTGAATGCG ATAAGTTGCG GCATGATGGC TACCGCAGTT CTCAGTACTA CTCTCAGGGA 
CCCACCTTTG CGGCCAACGC CAGCCCATTC TGTGATGATT ACCAAGATGA AGATGAAGAA 
ACAGATCAAA AGTGTTCTCT GTCTTCATCA GAAGAAGAAA GATTTATTTC CATCAGGAGA 
CCTAAAACAC CAGCCTCAAG TGACTTCTCC GACCTTAATA CTCAGACGAA CTGGACCAAG 
TCGCTTCCAC TGCCAACACC AGAAGAGAAG ATGCGACAGC AAGCCCAAAC AGTCCAGGCT 
GACGTGGTGC CTATTAACAT AACTGGGGAG AATTTCGATC GCCAGGCCAG CCTTCGGCGG 
TCTCTAATTT ACACAGACAC TCTGGTAAGA CGACCGAAGA AAGTCAAAAG GAGAAAGACT 
ATTACAGGAG TCCCTGACAA CATACAGAAG GAGCTAGCAT CAGGCACTGG CCAAGATGAT 
GCTGATGGCC ACTCAGTGTA CACCCCTGAT CACTACTCTA CACTAGGAAG GTTCAATAGC 
TGTCGGTCTG CTGGGCAGCG CTCAGAAACC AGGGACTCCA GCTGTCAGAC GGAGGATGTG 
AAGGTCGTAC CACCTTCCAT GAGGAGAATC AGGGCACAGA AGGGGCAAGG CATTGCTGCC 
CAGATGGGCC ACTTCTCAGG TTCCTCTGGC AACATGTCTG TGCTGAGCGA TTCTGCAGGG 
ATCGTATTCC CTTCCCGCCT TGACAGTGAT GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA 
GCAAGGGCAA ACATTCAGTC CCTTGAGCCG AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC 
ATGAATGGCA CTTTCCTCTA CCAGAGAGGT CACCCACAAG CAGATGAAAA CTTAGGCCAT 
TTAGGAGGTG CCTCAGGGAC TGGAACACTT TTGAGACCCA AATCCCAGGA GTTGAGACAC 
TTCGAGAGTG AAAATATAAT GAGCCCAGCG TGTGTGGTTT CTCCTCATGC AACCTACTCC 
ACCAGCATCA TCCCAAATGC CACACTGTCT TCCTCTTCCG AGGTCATCGC TATTCCCACT 
GCTCAGAGTG CGGGACAGCG GGAAAGTAAA AGTTCTGGCT CATCACATGC AAGGATAAAA 
TCCAGAGACC ACCTCATCTC CAGGCATGCT GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC 
CACTGGAATG AGGGCCATGC CACCATTCTT TCACAGGACT TAGACCCTCA TTCCCCTGGT 
GAACCCGCAC TGTTGTCCCT CTGTGACTCA GCCGTCCCTC TAAATGCTCC AGCAAATAGG 
GAGAATGGGT CCCAAGCTAT GCCGTATAAT TGTAGAAACA ACCTGGCCTT CCCAGCCCAC 
CCCCAAGATG TGGATGGCAA GAGTGAATCT AGTTATTCAG GGGGCGGAGG GCACAGCAGC 
TCGGAGCCCT GGGAATACAA ATCCTCAGGT AATGGAAGGG CATCCCCCCT GAAGCCGCAT 
TTAGCAACTC CTGGCTATTC CACTCCCACA AGTAACATGA GCAGCTGCAG TTTGGACCAA 
ACGTCCAACA AAGAGGATGC TGGGTCGCTG TATTCTGAGG ACCACGATGG CTACTGTGCA 
TCTGTGCACA CTGACTCTGG ACATGGATCT GGGAATCTGT GCAATAGCAG TGATGGCTTT 
GGGAACCCCA GGCACAGCGT GATCAATGTT TTTGTTGGAA GAGCTCAGAA AAACCAAGGG 
GACCGGTCCA ATTACCAGGA TAAATCCCTA TCAAGAAACA TCTCTTTGAA GAAAGCAAAG 
AAGCCTCCCC TGCCACCCTC CCGGACAGAC TCCCTCCGCA GGATTCCCAA GAAGAGCAGC 
CAGTGCAACG GGCAGGTGCT CAACGAGAGC CTGATCGCCA CACTCCAGCA CTCGCTGCAG 
CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG CCCTCCCAGA GCCCCTGCAG TGACTTGGAA 
GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG AGCACAGTTA GTGCTGGCAG CAGCATGACT 
TCCGCCACCA CCCCCAATGT CTACTCCCTG TGCGGGGCCA CGCCATCGCA GAGTGACACA 
AGCAGCGTCA AGTCAGAGTA CACGGACCCC TGGGGTTATT ACATTGACTA CACGGGCATG 
CAGGAAGATC CGGGGAACCC GGCAGGGGGC TGTTCAACCA GCAGTGGGGT GCCCACTGGG 
AACGGGCCAG TCCGCCATGT CCAAGAAGGG TCCAGAGCCA CAATGCCCCA AGTGCCCGGT 
GGTTCAGTCA AACCAAAGAT CATGTCACCA GAGAAGTCAC ACAGAGTCAT TTCTCCATCC 
AGTGGGTATT CCAGCCAGTC GAATACACCC ACAGCACTCA CCCCTGTGCC TGTGTTTTTA 
AAATCAGTGT CACCAGCAAA CGGGAAGGGG AAGCCCAAGC CCAAGGTACC AGAAAGGAAG 
TCCTCTCTGA TATCTTCAGT ATCCATTTCC TCATCGTCCA CTTCTCTTTC TTCTAGTACT 
TCTACTGAAG GAAGTGGCAC TATGAAGAAG CTGGATCCAG CCGTGGGCTC TCCCCCGGCT 
CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA TTCCCTTGTC CTGCAGACAG GTCTCCTTTC 
CTTCCTCCCC CACCTCCTGT CACAGATTGC TCCCAGGGCT CTCCTCTGCC TCACTCTCCT 
GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC ATTCCTTTCT GCTCCCCACC TGATTGGTGC 
CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC ATTCTTCCAG ATTCACCTGT GTCCTTGCCA 
TTGCCCCCAC CTCTCTTACC TTCCTCGGAA CCCCCACCTG CCCCACCTCT TGACCCCAAA 
TTCATGAAAG ACACCAGGCC GCCTTTCACA AATTCTGGCC AGCCAGAATC CTCCCGGGGA 
TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG ACCAGCAGGC CCCCCATGCC CCTGATAACC 
ACGGAAGCAT TGCAGATGGT GCAGTTGAGG CCCGTGAGAA AGAACTCAGG CGCTGAGGCG 
GCACAGTTGT CTGAACGAAC AGCTCAGGAA CAACGAACTC CAGTTGCTCC ACAGTACCAC 
TTAAAGCCAT CTGCTTTCCT GAAATCCCGA AATAGCACAA ATGAAATGGA GAGTGAAAGC 
CAGCCTGCCT CTGTGACAAG CTCGCTTCCG ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC 
CATGGCAGTG CGGCTGAGCG TGGTGGCCCT GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT 
GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC ACCACCCCAC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATTTC CAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AAAGATTTTG CAGTGGAGCC CGCAGAGAAC GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC 
CCCACGACGG GAGAGGAGGG CTCTGTGCAC AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC 
CAAGCTGGCT CTCATGCCAC GCACCCTGGC ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA 
TCCATGTCTC CCAGCAGAGT GGAAGCCAAT GTCCCCATGG TTCAGCCTGA TGTCTCACCA 
GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC AGTGCGGATA CTGGGGGCGA TGGGGAGAGC 
TGCCTATCTC AACAGGACGG AGCAGCTGGG GTGCCGGAGA CCAACGCAGC CGGTTCATCC 
TCAGAGGCCT GTGACTTCCT CAAGGAAGAC GGGAATGATG AGGTAATGAC CCCCAGTCGA 
CCCAGGACCA CAGAAGACCT TTTTGCAGCT ATTCACAGAT CCAAAAGGAA AGTCCTCGGC 
CGTAGAGATT CAGATGATGA CCACTCCCGA AACCATTCTC CCTCCCCGCC CGTGACACCC 
ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA AAGCAAGTGG GGTCGATTCA GAGAAGCATC 
CGAAAGAGCA GCACCAGCAG TGACAACTTC AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT 
TCAGACACCA GCGCCCGCAT GTCTGCAGCA GAGATGCTCA AGAACACAGA CCCTAGATTC 
CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA 
AGCAAGAACA GAAGGGCGCA GGAGGAGTGG GCCAAGAACG AAGGCTTGAT GCCTCGGAGT 
CTGTCCTTTT CCGGCCCCAG GTACGGCCGC AGCCGAACGC CGCCTTCTGC CGCCAGCAGC 
AGGTACAGCA TGCGGAACCG GATCCAGAGC AGCCCCATGA CCGTCATCTC GGAGGGAGAA 
GGGGAAGCCG TGGAGCCTGT GGACAGCATA GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA 
TGTTCCCTGG ACGGACTGGC GAGGGAGGAG ATGGACGAGG GCGGCCTGCT CTGTGGGGAG 
GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC CCCGGCCCTG TGGATGGGAC AGCCAGTGCA 
GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_017011088.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866577.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X9, mRNA.

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CloneID	OHu96700
Clone ID Related Accession (Same CDS sequence)	XM_017011088.1
Accession Version	XM_017011088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCAAA ACTTTCCTAG GAGCTCATAC TCATACACGT CAACACTACC CATGACCGGA 
AAAATGACAA CTGGCCTACC GAACTGGTCC TTCTGGCTTT TCACACTGCA GTGCTTGTTT 
GAAATACAGT TGACCCTTGA ACACAACACA TTTGAACTTG GAGGGTCCAC TTATATGGAT 
TTTTTCAATA CAGTCAGCCC TCCATACATT CTGGGTCCTG CAACCGAAAC CAAATGCGAA 
TGGAAAATAC AATACTCAGA ATGCGATAAG TTGCGGCATG ATGGCTACCG CAGTTCTCAG 
TACTACTCTC AGGGACCCAC CTTTGCGGCC AACGCCAGCC CATTCTGTGA TGATTACCAA 
GATGAAGATG AAGAAACAGA TCAAAAGTGT TCTCTGTCTT CATCAGAAGA AGAAAGATTT 
ATTTCCATCA GGAGACCTAA AACACCAGCC TCAAGTGACT TCTCCGACCT TAATACTCAG 
ACGAACTGGA CCAAGTCGCT TCCACTGCCA ACACCAGAAG AGAAGATGCG ACAGCAAGCC 
CAAACAGTCC AGGCTGACGT GGTGCCTATT AACATAACTG GGGAGAATTT CGATCGCCAG 
GCCAGCCTTC GGCGGTCTCT AATTTACACA GACACTCTGG TAAGACGACC GAAGAAAGTC 
AAAAGGAGAA AGACTATTAC AGGAGTCCCT GACAACATAC AGAAGGAGCT AGCATCAGGC 
ACTGGCCAAG ATGATGCTGA TGGCCACTCA GTGTACACCC CTGATCACTA CTCTACACTA 
GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG CAGCGCTCAG AAACCAGGGA CTCCAGCTGT 
CAGACGGAGG ATGTGAAGGT CGTACCACCT TCCATGAGGA GAATCAGGGC ACAGAAGGGG 
CAAGGCATTG CTGCCCAGAT GGGCCACTTC TCAGGTTCCT CTGGCAACAT GTCTGTGCTG 
AGCGATTCTG CAGGGATCGT ATTCCCTTCC CGCCTTGACA GTGATGCTGG CTTCCATAGT 
CTTCCGCGTT CTGGAGCAAG GGCAAACATT CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC 
GGCCCTGCAG GAGACATGAA TGGCACTTTC CTCTACCAGA GAGGTCACCC ACAAGCAGAT 
GAAAACTTAG GCCATTTAGG AGGTGCCTCA GGGACTGGAA CACTTTTGAG ACCCAAATCC 
CAGGAGTTGA GACACTTCGA GAGTGAAAAT ATAATGAGCC CAGCGTGTGT GGTTTCTCCT 
CATGCAACCT ACTCCACCAG CATCATCCCA AATGCCACAC TGTCTTCCTC TTCCGAGGTC 
ATCGCTATTC CCACTGCTCA GAGTGCGGGA CAGCGGGAAA GTAAAAGTTC TGGCTCATCA 
CATGCAAGGA TAAAATCCAG AGACCACCTC ATCTCCAGGC ATGCTGTGAA AGGTGATCCT 
CAGTCTCCCG GTCGCCACTG GAATGAGGGC CATGCCACCA TTCTTTCACA GGACTTAGAC 
CCTCATTCCC CTGGTGAACC CGCACTGTTG TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT 
GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA GCTATGCCGT ATAATTGTAG AAACAACCTG 
GCCTTCCCAG CCCACCCCCA AGATGTGGAT GGCAAGAGTG AATCTAGTTA TTCAGGGGGC 
GGAGGGCACA GCAGCTCGGA GCCCTGGGAA TACAAATCCT CAGGTAATGG AAGGGCATCC 
CCCCTGAAGC CGCATTTAGC AACTCCTGGC TATTCCACTC CCACAAGTAA CATGAGCAGC 
TGCAGTTTGG ACCAAACGTC CAACAAAGAG GATGCTGGGT CGCTGTATTC TGAGGACCAC 
GATGGCTACT GTGCATCTGT GCACACTGAC TCTGGACATG GATCTGGGAA TCTGTGCAAT 
AGCAGTGATG GCTTTGGGAA CCCCAGGCAC AGCGTGATCA ATGTTTTTGT TGGAAGAGCT 
CAGAAAAACC AAGGGGACCG GTCCAATTAC CAGGATAAAT CCCTATCAAG AAACATCTCT 
TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA CCCTCCCGGA CAGACTCCCT CCGCAGGATT 
CCCAAGAAGA GCAGCCAGTG CAACGGGCAG GTGCTCAACG AGAGCCTGAT CGCCACACTC 
CAGCACTCGC TGCAGCTGAG CCTCCCAGGC AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC 
TGCAGTGACT TGGAAGAGCC CTGGCTGCCC CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT 
GGCAGCAGCA TGACTTCCGC CACCACCCCC AATGTCTACT CCCTGTGCGG GGCCACGCCA 
TCGCAGAGTG ACACAAGCAG CGTCAAGTCA GAGTACACGG ACCCCTGGGG TTATTACATT 
GACTACACGG GCATGCAGGA AGATCCGGGG AACCCGGCAG GGGGCTGTTC AACCAGCAGT 
GGGGTGCCCA CTGGGAACGG GCCAGTCCGC CATGTCCAAG AAGGGTCCAG AGCCACAATG 
CCCCAAGTGC CCGGTGGTTC AGTCAAACCA AAGATCATGT CACCAGAGAA GTCACACAGA 
GTCATTTCTC CATCCAGTGG GTATTCCAGC CAGTCGAATA CACCCACAGC ACTCACCCCT 
GTGCCTGTGT TTTTAAAATC AGTGTCACCA GCAAACGGGA AGGGGAAGCC CAAGCCCAAG 
GTACCAGAAA GGAAGTCCTC TCTGATATCT TCAGTATCCA TTTCCTCATC GTCCACTTCT 
CTTTCTTCTA GTACTTCTAC TGAAGGAAGT GGCACTATGA AGAAGCTGGA TCCAGCCGTG 
GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT GTTCCCTCTC CTCCATTCCC TTGTCCTGCA 
GACAGGTCTC CTTTCCTTCC TCCCCCACCT CCTGTCACAG ATTGCTCCCA GGGCTCTCCT 
CTGCCTCACT CTCCTGTGTT CCCCCCTCCG CCGCCAGAAG CTCTCATTCC TTTCTGCTCC 
CCACCTGATT GGTGCCTTTC TCCTCCCCGC CCTGCACTGA GCCCCATTCT TCCAGATTCA 
CCTGTGTCCT TGCCATTGCC CCCACCTCTC TTACCTTCCT CGGAACCCCC ACCTGCCCCA 
CCTCTTGACC CCAAATTCAT GAAAGACACC AGGCCGCCTT TCACAAATTC TGGCCAGCCA 
GAATCCTCCC GGGGATCCTT GAGGCCGCCT TCTACCAAGG AGGAGACCAG CAGGCCCCCC 
ATGCCCCTGA TAACCACGGA AGCATTGCAG ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC 
TCAGGCGCTG AGGCGGCACA GTTGTCTGAA CGAACAGCTC AGGAACAACG AACTCCAGTT 
GCTCCACAGT ACCACTTAAA GCCATCTGCT TTCCTGAAAT CCCGAAATAG CACAAATGAA 
ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG ACAAGCTCGC TTCCGACGCC TGCCAAGAGC 
TCGAGTCAGG GTGACCATGG CAGTGCGGCT GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT 
GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT CGGCCCAGCC CCAGCACCAC CCCACTCCCA 
GACTCTTCAC CCAGCAGGAA GCCACCCCCC ATTTCCAAGA AGCCCAAACT GTTCCTGGTG 
GTACCACCTC CGCAGAAAGA TTTTGCAGTG GAGCCCGCAG AGAACGTGAG CGAAGCCCTC 
CGAGCTGTGC CCAGCCCCAC GACGGGAGAG GAGGGCTCTG TGCACAGCAG GGAGGCAAAA 
GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT GCCACGCACC CTGGCACCTC GGTTCTTGAG 
GGAGGAGCTG CAGGATCCAT GTCTCCCAGC AGAGTGGAAG CCAATGTCCC CATGGTTCAG 
CCTGATGTCT CACCAGCCCC CAAGCAGGAG GAGCCAGCCG AGAACAGTGC GGATACTGGG 
GGCGATGGGG AGAGCTGCCT ATCTCAACAG GACGGAGCAG CTGGGGTGCC GGAGACCAAC 
GCAGCCGGTT CATCCTCAGA GGCCTGTGAC TTCCTCAAGG AAGACGGGAA TGATGAGGTA 
ATGACCCCCA GTCGACCCAG GACCACAGAA GACCTTTTTG CAGCTATTCA CAGATCCAAA 
AGGAAAGTCC TCGGCCGTAG AGATTCAGAT GATGACCACT CCCGAAACCA TTCTCCCTCC 
CCGCCCGTGA CACCCACCGG CGCTGCCCCA AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG 
ATTCAGAGAA GCATCCGAAA GAGCAGCACC AGCAGTGACA ACTTCAAAGC TCTGCTGCTG 
AAAAAGGGCA GTCGTTCAGA CACCAGCGCC CGCATGTCTG CAGCAGAGAT GCTCAAGAAC 
ACAGACCCTA GATTCCAGAG GTCGAGGTCA GAGCCTTCCC CAGATGCCCC CGAGAGCCCG 
TCAAGCTGCT CCCCAAGCAA GAACAGAAGG GCGCAGGAGG AGTGGGCCAA GAACGAAGGC 
TTGATGCCTC GGAGTCTGTC CTTTTCCGGC CCCAGGTACG GCCGCAGCCG AACGCCGCCT 
TCTGCCGCCA GCAGCAGGTA CAGCATGCGG AACCGGATCC AGAGCAGCCC CATGACCGTC 
ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG CCTGTGGACA GCATAGCCCG CGGGGCTCTG 
GGCGCTGCGG AGGGATGTTC CCTGGACGGA CTGGCGAGGG AGGAGATGGA CGAGGGCGGC 
CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT 
GGGACAGCCA GTGCAGAGGG CAGAGAGCCC TCCCCACAGT GTGGCGGTTC TCTGAGCGAG 
GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866577.1
CDS	1637..6505
Translation	MFQNFPRSSYSYTSTLPMTGKMTTGLPNWSFWLFTLQCLFEIQLTLEHNTFELGGSTYMDFFNTVSPPYILGPATETKCEWKIQYSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_017011088.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011088.1

ORF Insert Sequence:

ATGTTTCAAA ACTTTCCTAG GAGCTCATAC TCATACACGT CAACACTACC CATGACCGGA 
AAAATGACAA CTGGCCTACC GAACTGGTCC TTCTGGCTTT TCACACTGCA GTGCTTGTTT 
GAAATACAGT TGACCCTTGA ACACAACACA TTTGAACTTG GAGGGTCCAC TTATATGGAT 
TTTTTCAATA CAGTCAGCCC TCCATACATT CTGGGTCCTG CAACCGAAAC CAAATGCGAA 
TGGAAAATAC AATACTCAGA ATGCGATAAG TTGCGGCATG ATGGCTACCG CAGTTCTCAG 
TACTACTCTC AGGGACCCAC CTTTGCGGCC AACGCCAGCC CATTCTGTGA TGATTACCAA 
GATGAAGATG AAGAAACAGA TCAAAAGTGT TCTCTGTCTT CATCAGAAGA AGAAAGATTT 
ATTTCCATCA GGAGACCTAA AACACCAGCC TCAAGTGACT TCTCCGACCT TAATACTCAG 
ACGAACTGGA CCAAGTCGCT TCCACTGCCA ACACCAGAAG AGAAGATGCG ACAGCAAGCC 
CAAACAGTCC AGGCTGACGT GGTGCCTATT AACATAACTG GGGAGAATTT CGATCGCCAG 
GCCAGCCTTC GGCGGTCTCT AATTTACACA GACACTCTGG TAAGACGACC GAAGAAAGTC 
AAAAGGAGAA AGACTATTAC AGGAGTCCCT GACAACATAC AGAAGGAGCT AGCATCAGGC 
ACTGGCCAAG ATGATGCTGA TGGCCACTCA GTGTACACCC CTGATCACTA CTCTACACTA 
GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG CAGCGCTCAG AAACCAGGGA CTCCAGCTGT 
CAGACGGAGG ATGTGAAGGT CGTACCACCT TCCATGAGGA GAATCAGGGC ACAGAAGGGG 
CAAGGCATTG CTGCCCAGAT GGGCCACTTC TCAGGTTCCT CTGGCAACAT GTCTGTGCTG 
AGCGATTCTG CAGGGATCGT ATTCCCTTCC CGCCTTGACA GTGATGCTGG CTTCCATAGT 
CTTCCGCGTT CTGGAGCAAG GGCAAACATT CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC 
GGCCCTGCAG GAGACATGAA TGGCACTTTC CTCTACCAGA GAGGTCACCC ACAAGCAGAT 
GAAAACTTAG GCCATTTAGG AGGTGCCTCA GGGACTGGAA CACTTTTGAG ACCCAAATCC 
CAGGAGTTGA GACACTTCGA GAGTGAAAAT ATAATGAGCC CAGCGTGTGT GGTTTCTCCT 
CATGCAACCT ACTCCACCAG CATCATCCCA AATGCCACAC TGTCTTCCTC TTCCGAGGTC 
ATCGCTATTC CCACTGCTCA GAGTGCGGGA CAGCGGGAAA GTAAAAGTTC TGGCTCATCA 
CATGCAAGGA TAAAATCCAG AGACCACCTC ATCTCCAGGC ATGCTGTGAA AGGTGATCCT 
CAGTCTCCCG GTCGCCACTG GAATGAGGGC CATGCCACCA TTCTTTCACA GGACTTAGAC 
CCTCATTCCC CTGGTGAACC CGCACTGTTG TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT 
GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA GCTATGCCGT ATAATTGTAG AAACAACCTG 
GCCTTCCCAG CCCACCCCCA AGATGTGGAT GGCAAGAGTG AATCTAGTTA TTCAGGGGGC 
GGAGGGCACA GCAGCTCGGA GCCCTGGGAA TACAAATCCT CAGGTAATGG AAGGGCATCC 
CCCCTGAAGC CGCATTTAGC AACTCCTGGC TATTCCACTC CCACAAGTAA CATGAGCAGC 
TGCAGTTTGG ACCAAACGTC CAACAAAGAG GATGCTGGGT CGCTGTATTC TGAGGACCAC 
GATGGCTACT GTGCATCTGT GCACACTGAC TCTGGACATG GATCTGGGAA TCTGTGCAAT 
AGCAGTGATG GCTTTGGGAA CCCCAGGCAC AGCGTGATCA ATGTTTTTGT TGGAAGAGCT 
CAGAAAAACC AAGGGGACCG GTCCAATTAC CAGGATAAAT CCCTATCAAG AAACATCTCT 
TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA CCCTCCCGGA CAGACTCCCT CCGCAGGATT 
CCCAAGAAGA GCAGCCAGTG CAACGGGCAG GTGCTCAACG AGAGCCTGAT CGCCACACTC 
CAGCACTCGC TGCAGCTGAG CCTCCCAGGC AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC 
TGCAGTGACT TGGAAGAGCC CTGGCTGCCC CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT 
GGCAGCAGCA TGACTTCCGC CACCACCCCC AATGTCTACT CCCTGTGCGG GGCCACGCCA 
TCGCAGAGTG ACACAAGCAG CGTCAAGTCA GAGTACACGG ACCCCTGGGG TTATTACATT 
GACTACACGG GCATGCAGGA AGATCCGGGG AACCCGGCAG GGGGCTGTTC AACCAGCAGT 
GGGGTGCCCA CTGGGAACGG GCCAGTCCGC CATGTCCAAG AAGGGTCCAG AGCCACAATG 
CCCCAAGTGC CCGGTGGTTC AGTCAAACCA AAGATCATGT CACCAGAGAA GTCACACAGA 
GTCATTTCTC CATCCAGTGG GTATTCCAGC CAGTCGAATA CACCCACAGC ACTCACCCCT 
GTGCCTGTGT TTTTAAAATC AGTGTCACCA GCAAACGGGA AGGGGAAGCC CAAGCCCAAG 
GTACCAGAAA GGAAGTCCTC TCTGATATCT TCAGTATCCA TTTCCTCATC GTCCACTTCT 
CTTTCTTCTA GTACTTCTAC TGAAGGAAGT GGCACTATGA AGAAGCTGGA TCCAGCCGTG 
GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT GTTCCCTCTC CTCCATTCCC TTGTCCTGCA 
GACAGGTCTC CTTTCCTTCC TCCCCCACCT CCTGTCACAG ATTGCTCCCA GGGCTCTCCT 
CTGCCTCACT CTCCTGTGTT CCCCCCTCCG CCGCCAGAAG CTCTCATTCC TTTCTGCTCC 
CCACCTGATT GGTGCCTTTC TCCTCCCCGC CCTGCACTGA GCCCCATTCT TCCAGATTCA 
CCTGTGTCCT TGCCATTGCC CCCACCTCTC TTACCTTCCT CGGAACCCCC ACCTGCCCCA 
CCTCTTGACC CCAAATTCAT GAAAGACACC AGGCCGCCTT TCACAAATTC TGGCCAGCCA 
GAATCCTCCC GGGGATCCTT GAGGCCGCCT TCTACCAAGG AGGAGACCAG CAGGCCCCCC 
ATGCCCCTGA TAACCACGGA AGCATTGCAG ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC 
TCAGGCGCTG AGGCGGCACA GTTGTCTGAA CGAACAGCTC AGGAACAACG AACTCCAGTT 
GCTCCACAGT ACCACTTAAA GCCATCTGCT TTCCTGAAAT CCCGAAATAG CACAAATGAA 
ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG ACAAGCTCGC TTCCGACGCC TGCCAAGAGC 
TCGAGTCAGG GTGACCATGG CAGTGCGGCT GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT 
GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT CGGCCCAGCC CCAGCACCAC CCCACTCCCA 
GACTCTTCAC CCAGCAGGAA GCCACCCCCC ATTTCCAAGA AGCCCAAACT GTTCCTGGTG 
GTACCACCTC CGCAGAAAGA TTTTGCAGTG GAGCCCGCAG AGAACGTGAG CGAAGCCCTC 
CGAGCTGTGC CCAGCCCCAC GACGGGAGAG GAGGGCTCTG TGCACAGCAG GGAGGCAAAA 
GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT GCCACGCACC CTGGCACCTC GGTTCTTGAG 
GGAGGAGCTG CAGGATCCAT GTCTCCCAGC AGAGTGGAAG CCAATGTCCC CATGGTTCAG 
CCTGATGTCT CACCAGCCCC CAAGCAGGAG GAGCCAGCCG AGAACAGTGC GGATACTGGG 
GGCGATGGGG AGAGCTGCCT ATCTCAACAG GACGGAGCAG CTGGGGTGCC GGAGACCAAC 
GCAGCCGGTT CATCCTCAGA GGCCTGTGAC TTCCTCAAGG AAGACGGGAA TGATGAGGTA 
ATGACCCCCA GTCGACCCAG GACCACAGAA GACCTTTTTG CAGCTATTCA CAGATCCAAA 
AGGAAAGTCC TCGGCCGTAG AGATTCAGAT GATGACCACT CCCGAAACCA TTCTCCCTCC 
CCGCCCGTGA CACCCACCGG CGCTGCCCCA AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG 
ATTCAGAGAA GCATCCGAAA GAGCAGCACC AGCAGTGACA ACTTCAAAGC TCTGCTGCTG 
AAAAAGGGCA GTCGTTCAGA CACCAGCGCC CGCATGTCTG CAGCAGAGAT GCTCAAGAAC 
ACAGACCCTA GATTCCAGAG GTCGAGGTCA GAGCCTTCCC CAGATGCCCC CGAGAGCCCG 
TCAAGCTGCT CCCCAAGCAA GAACAGAAGG GCGCAGGAGG AGTGGGCCAA GAACGAAGGC 
TTGATGCCTC GGAGTCTGTC CTTTTCCGGC CCCAGGTACG GCCGCAGCCG AACGCCGCCT 
TCTGCCGCCA GCAGCAGGTA CAGCATGCGG AACCGGATCC AGAGCAGCCC CATGACCGTC 
ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG CCTGTGGACA GCATAGCCCG CGGGGCTCTG 
GGCGCTGCGG AGGGATGTTC CCTGGACGGA CTGGCGAGGG AGGAGATGGA CGAGGGCGGC 
CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT 
GGGACAGCCA GTGCAGAGGG CAGAGAGCCC TCCCCACAGT GTGGCGGTTC TCTGAGCGAG 
GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_017011089.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866578.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X14, mRNA.

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CloneID	OHu96701
Clone ID Related Accession (Same CDS sequence)	XM_017011089.1
Accession Version	XM_017011089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGTC TGAAAGCGGT TTCCAACCTA GATGAGGAAA GCCGATGGAC AGTCCACTAC 
ACTGCCCCGT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TACTGAGGGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866578.1
CDS	728..5506
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_017011089.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011089.1

ORF Insert Sequence:

ATGTTCTGTC TGAAAGCGGT TTCCAACCTA GATGAGGAAA GCCGATGGAC AGTCCACTAC 
ACTGCCCCGT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TACTGAGGGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( NM_001144060.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137532.1 Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.

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CloneID	OHu15007
Clone ID Related Accession (Same CDS sequence)	NM_001144060.1 , NM_001144060.2
Accession Version	NM_001144060.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591375.8 and AL391669.11. On or before Jan 23, 2009 this sequence version replaced XM_001723403.1, XM_496826.4, XM_941807.3. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct start codon, and includes an alternate in-frame exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus and includes an alternate segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGTCT TCATTAATGC AAAGATTAAG TCTTTAATTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACC TAGATGAGGA AAGCCGATGG ACAGTCCACT ACACTGCCCC GTGGCACCAG 
CAGGAGAATG TGTTCCTTCC CACCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTACTGAGG GAATGCGATA AGTTGCGGCA TGATGGCTAC 
CGCAGTTCTC AGTACTACTC TCAGGGACCC ACCTTTGCGG CCAACGCCAG CCCATTCTGT 
GATGATTACC AAGATGAAGA TGAAGAAACA GATCAAAAGT GTTCTCTGTC TTCATCAGAA 
GAAGAAAGAT TTATTTCCAT CAGGAGACCT AAAACACCAG CCTCAAGTGA CTTCTCCGAC 
CTTAATACTC AGACGAACTG GACCAAGTCG CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CCCAAACAGT CCAGGCTGAC GTGGTGCCTA TTAACATAAC TGGGGAGAAT 
TTCGATCGCC AGGCCAGCCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA 
CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG 
CTAGCATCAG GCACTGGCCA AGATGATGCT GATGGCCACT CAGTGTACAC CCCTGATCAC 
TACTCTACAC TAGGAAGGTT CAATAGCTGT CGGTCTGCTG GGCAGCGCTC AGAAACCAGG 
GACTCCAGCT GTCAGACGGA GGATGTGAAG GTCGTACCAC CTTCCATGAG GAGAATCAGG 
GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGGGCCACT TCTCAGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGATTC TGCAGGGATC GTATTCCCTT CCCGCCTTGA CAGTGATGCT 
GGCTTCCATA GTCTTCCGCG TTCTGGAGCA AGGGCAAACA TTCAGTCCCT TGAGCCGAGG 
CTGGGTGCCC TCGGCCCTGC AGGAGACATG AATGGCACTT TCCTCTACCA GAGAGGTCAC 
CCACAAGCAG ATGAAAACTT AGGCCATTTA GGAGGTGCCT CAGGGACTGG AACACTTTTG 
AGACCCAAAT CCCAGGAGTT GAGACACTTC GAGAGTGAAA ATATAATGAG CCCAGCGTGT 
GTGGTTTCTC CTCATGCAAC CTACTCCACC AGCATCATCC CAAATGCCAC ACTGTCTTCC 
TCTTCCGAGG TCATCGCTAT TCCCACTGCT CAGAGTGCGG GACAGCGGGA AAGTAAAAGT 
TCTGGCTCAT CACATGCAAG GATAAAATCC AGAGACCACC TCATCTCCAG GCATGCTGTG 
AAAGGTGATC CTCAGTCTCC CGGTCGCCAC TGGAATGAGG GCCATGCCAC CATTCTTTCA 
CAGGACTTAG ACCCTCATTC CCCTGGTGAA CCCGCACTGT TGTCCCTCTG TGACTCAGCC 
GTCCCTCTAA ATGCTCCAGC AAATAGGGAG AATGGGTCCC AAGCTATGCC GTATAATTGT 
AGAAACAACC TGGCCTTCCC AGCCCACCCC CAAGATGTGG ATGGCAAGAG TGAATCTAGT 
TATTCAGGGG GCGGAGGGCA CAGCAGCTCG GAGCCCTGGG AATACAAATC CTCAGGTAAT 
GGAAGGGCAT CCCCCCTGAA GCCGCATTTA GCAACTCCTG GCTATTCCAC TCCCACAAGT 
AACATGAGCA GCTGCAGTTT GGACCAAACG TCCAACAAAG AGGATGCTGG GTCGCTGTAT 
TCTGAGGACC ACGATGGCTA CTGTGCATCT GTGCACACTG ACTCTGGACA TGGATCTGGG 
AATCTGTGCA ATAGCAGTGA TGGCTTTGGG AACCCCAGGC ACAGCGTGAT CAATGTTTTT 
GTTGGAAGAG CTCAGAAAAA CCAAGGGGAC CGGTCCAATT ACCAGGATAA ATCCCTATCA 
AGAAACATCT CTTTGAAGAA AGCAAAGAAG CCTCCCCTGC CACCCTCCCG GACAGACTCC 
CTCCGCAGGA TTCCCAAGAA GAGCAGCCAG TGCAACGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCACAC TCCAGCACTC GCTGCAGCTG AGCCTCCCAG GCAAGAGTGG CAGCTCGCCC 
TCCCAGAGCC CCTGCAGTGA CTTGGAAGAG CCCTGGCTGC CCCGCTCCCG GAGCCAGAGC 
ACAGTTAGTG CTGGCAGCAG CATGACTTCC GCCACCACCC CCAATGTCTA CTCCCTGTGC 
GGGGCCACGC CATCGCAGAG TGACACAAGC AGCGTCAAGT CAGAGTACAC GGACCCCTGG 
GGTTATTACA TTGACTACAC GGGCATGCAG GAAGATCCGG GGAACCCGGC AGGGGGCTGT 
TCAACCAGCA GTGGGGTGCC CACTGGGAAC GGGCCAGTCC GCCATGTCCA AGAAGGGTCC 
AGAGCCACAA TGCCCCAAGT GCCCGGTGGT TCAGTCAAAC CAAAGATCAT GTCACCAGAG 
AAGTCACACA GAGTCATTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA 
GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAACGG GAAGGGGAAG 
CCCAAGCCCA AGGTACCAGA AAGGAAGTCC TCTCTGATAT CTTCAGTATC CATTTCCTCA 
TCGTCCACTT CTCTTTCTTC TAGTACTTCT ACTGAAGGAA GTGGCACTAT GAAGAAGCTG 
GATCCAGCCG TGGGCTCTCC CCCGGCTCCT CCTCCTCCTC CTGTTCCCTC TCCTCCATTC 
CCTTGTCCTG CAGACAGGTC TCCTTTCCTT CCTCCCCCAC CTCCTGTCAC AGATTGCTCC 
CAGGGCTCTC CTCTGCCTCA CTCTCCTGTG TTCCCCCCTC CGCCGCCAGA AGCTCTCATT 
CCTTTCTGCT CCCCACCTGA TTGGTGCCTT TCTCCTCCCC GCCCTGCACT GAGCCCCATT 
CTTCCAGATT CACCTGTGTC CTTGCCATTG CCCCCACCTC TCTTACCTTC CTCGGAACCC 
CCACCTGCCC CACCTCTTGA CCCCAAATTC ATGAAAGACA CCAGGCCGCC TTTCACAAAT 
TCTGGCCAGC CAGAATCCTC CCGGGGATCC TTGAGGCCGC CTTCTACCAA GGAGGAGACC 
AGCAGGCCCC CCATGCCCCT GATAACCACG GAAGCATTGC AGATGGTGCA GTTGAGGCCC 
GTGAGAAAGA ACTCAGGCGC TGAGGCGGCA CAGTTGTCTG AACGAACAGC TCAGGAACAA 
CGAACTCCAG TTGCTCCACA GTACCACTTA AAGCCATCTG CTTTCCTGAA ATCCCGAAAT 
AGCACAAATG AAATGGAGAG TGAAAGCCAG CCTGCCTCTG TGACAAGCTC GCTTCCGACG 
CCTGCCAAGA GCTCGAGTCA GGGTGACCAT GGCAGTGCGG CTGAGCGTGG TGGCCCTGTG 
AGCCGCAGCC CTGGAGCTCC AAGCGCTGGG GAGGCAGAGG CTCGGCCCAG CCCCAGCACC 
ACCCCACTCC CAGACTCTTC ACCCAGCAGG AAGCCACCCC CCATTTCCAA GAAGCCCAAA 
CTGTTCCTGG TGGTACCACC TCCGCAGAAA GATTTTGCAG TGGAGCCCGC AGAGAACGTG 
AGCGAAGCCC TCCGAGCTGT GCCCAGCCCC ACGACGGGAG AGGAGGGCTC TGTGCACAGC 
AGGGAGGCAA AAGAGAGTTC TGCAGCCCAA GCTGGCTCTC ATGCCACGCA CCCTGGCACC 
TCGGTTCTTG AGGGAGGAGC TGCAGGATCC ATGTCTCCCA GCAGAGTGGA AGCCAATGTC 
CCCATGGTTC AGCCTGATGT CTCACCAGCC CCCAAGCAGG AGGAGCCAGC CGAGAACAGT 
GCGGATACTG GGGGCGATGG GGAGAGCTGC CTATCTCAAC AGGACGGAGC AGCTGGGGTG 
CCGGAGACCA ACGCAGCCGG TTCATCCTCA GAGGCCTGTG ACTTCCTCAA GGAAGACGGG 
AATGATGAGG TAATGACCCC CAGTCGACCC AGGACCACAG AAGACCTTTT TGCAGCTATT 
CACAGATCCA AAAGGAAAGT CCTCGGCCGT AGAGATTCAG ATGATGACCA CTCCCGAAAC 
CATTCTCCCT CCCCGCCCGT GACACCCACC GGCGCTGCCC CAAGCCTGGC CTCTCCAAAG 
CAAGTGGGGT CGATTCAGAG AAGCATCCGA AAGAGCAGCA CCAGCAGTGA CAACTTCAAA 
GCTCTGCTGC TGAAAAAGGG CAGTCGTTCA GACACCAGCG CCCGCATGTC TGCAGCAGAG 
ATGCTCAAGA ACACAGACCC TAGATTCCAG AGGTCGAGGT CAGAGCCTTC CCCAGATGCC 
CCCGAGAGCC CGTCAAGCTG CTCCCCAAGC AAGAACAGAA GGGCGCAGGA GGAGTGGGCC 
AAGAACGAAG GCTTGATGCC TCGGAGTCTG TCCTTTTCCG GCCCCAGGTA CGGCCGCAGC 
CGAACGCCGC CTTCTGCCGC CAGCAGCAGG TACAGCATGC GGAACCGGAT CCAGAGCAGC 
CCCATGACCG TCATCTCGGA GGGAGAAGGG GAAGCCGTGG AGCCTGTGGA CAGCATAGCC 
CGCGGGGCTC TGGGCGCTGC GGAGGGATGT TCCCTGGACG GACTGGCGAG GGAGGAGATG 
GACGAGGGCG GCCTGCTCTG TGGGGAGGGG CCTGCCGCCT CCCTGCAGCC CCAGGCCCCC 
GGCCCTGTGG ATGGGACAGC CAGTGCAGAG GGCAGAGAGC CCTCCCCACA GTGTGGCGGT 
TCTCTGAGCG AGGAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137532.1
CDS	153..4973
Translation	MVVFINAKIKSLIKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	NM_001144060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144060.1

ORF Insert Sequence:

ATGGTGGTCT TCATTAATGC AAAGATTAAG TCTTTAATTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACC TAGATGAGGA AAGCCGATGG ACAGTCCACT ACACTGCCCC GTGGCACCAG 
CAGGAGAATG TGTTCCTTCC CACCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTACTGAGG GAATGCGATA AGTTGCGGCA TGATGGCTAC 
CGCAGTTCTC AGTACTACTC TCAGGGACCC ACCTTTGCGG CCAACGCCAG CCCATTCTGT 
GATGATTACC AAGATGAAGA TGAAGAAACA GATCAAAAGT GTTCTCTGTC TTCATCAGAA 
GAAGAAAGAT TTATTTCCAT CAGGAGACCT AAAACACCAG CCTCAAGTGA CTTCTCCGAC 
CTTAATACTC AGACGAACTG GACCAAGTCG CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CCCAAACAGT CCAGGCTGAC GTGGTGCCTA TTAACATAAC TGGGGAGAAT 
TTCGATCGCC AGGCCAGCCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA 
CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG 
CTAGCATCAG GCACTGGCCA AGATGATGCT GATGGCCACT CAGTGTACAC CCCTGATCAC 
TACTCTACAC TAGGAAGGTT CAATAGCTGT CGGTCTGCTG GGCAGCGCTC AGAAACCAGG 
GACTCCAGCT GTCAGACGGA GGATGTGAAG GTCGTACCAC CTTCCATGAG GAGAATCAGG 
GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGGGCCACT TCTCAGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGATTC TGCAGGGATC GTATTCCCTT CCCGCCTTGA CAGTGATGCT 
GGCTTCCATA GTCTTCCGCG TTCTGGAGCA AGGGCAAACA TTCAGTCCCT TGAGCCGAGG 
CTGGGTGCCC TCGGCCCTGC AGGAGACATG AATGGCACTT TCCTCTACCA GAGAGGTCAC 
CCACAAGCAG ATGAAAACTT AGGCCATTTA GGAGGTGCCT CAGGGACTGG AACACTTTTG 
AGACCCAAAT CCCAGGAGTT GAGACACTTC GAGAGTGAAA ATATAATGAG CCCAGCGTGT 
GTGGTTTCTC CTCATGCAAC CTACTCCACC AGCATCATCC CAAATGCCAC ACTGTCTTCC 
TCTTCCGAGG TCATCGCTAT TCCCACTGCT CAGAGTGCGG GACAGCGGGA AAGTAAAAGT 
TCTGGCTCAT CACATGCAAG GATAAAATCC AGAGACCACC TCATCTCCAG GCATGCTGTG 
AAAGGTGATC CTCAGTCTCC CGGTCGCCAC TGGAATGAGG GCCATGCCAC CATTCTTTCA 
CAGGACTTAG ACCCTCATTC CCCTGGTGAA CCCGCACTGT TGTCCCTCTG TGACTCAGCC 
GTCCCTCTAA ATGCTCCAGC AAATAGGGAG AATGGGTCCC AAGCTATGCC GTATAATTGT 
AGAAACAACC TGGCCTTCCC AGCCCACCCC CAAGATGTGG ATGGCAAGAG TGAATCTAGT 
TATTCAGGGG GCGGAGGGCA CAGCAGCTCG GAGCCCTGGG AATACAAATC CTCAGGTAAT 
GGAAGGGCAT CCCCCCTGAA GCCGCATTTA GCAACTCCTG GCTATTCCAC TCCCACAAGT 
AACATGAGCA GCTGCAGTTT GGACCAAACG TCCAACAAAG AGGATGCTGG GTCGCTGTAT 
TCTGAGGACC ACGATGGCTA CTGTGCATCT GTGCACACTG ACTCTGGACA TGGATCTGGG 
AATCTGTGCA ATAGCAGTGA TGGCTTTGGG AACCCCAGGC ACAGCGTGAT CAATGTTTTT 
GTTGGAAGAG CTCAGAAAAA CCAAGGGGAC CGGTCCAATT ACCAGGATAA ATCCCTATCA 
AGAAACATCT CTTTGAAGAA AGCAAAGAAG CCTCCCCTGC CACCCTCCCG GACAGACTCC 
CTCCGCAGGA TTCCCAAGAA GAGCAGCCAG TGCAACGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCACAC TCCAGCACTC GCTGCAGCTG AGCCTCCCAG GCAAGAGTGG CAGCTCGCCC 
TCCCAGAGCC CCTGCAGTGA CTTGGAAGAG CCCTGGCTGC CCCGCTCCCG GAGCCAGAGC 
ACAGTTAGTG CTGGCAGCAG CATGACTTCC GCCACCACCC CCAATGTCTA CTCCCTGTGC 
GGGGCCACGC CATCGCAGAG TGACACAAGC AGCGTCAAGT CAGAGTACAC GGACCCCTGG 
GGTTATTACA TTGACTACAC GGGCATGCAG GAAGATCCGG GGAACCCGGC AGGGGGCTGT 
TCAACCAGCA GTGGGGTGCC CACTGGGAAC GGGCCAGTCC GCCATGTCCA AGAAGGGTCC 
AGAGCCACAA TGCCCCAAGT GCCCGGTGGT TCAGTCAAAC CAAAGATCAT GTCACCAGAG 
AAGTCACACA GAGTCATTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA 
GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAACGG GAAGGGGAAG 
CCCAAGCCCA AGGTACCAGA AAGGAAGTCC TCTCTGATAT CTTCAGTATC CATTTCCTCA 
TCGTCCACTT CTCTTTCTTC TAGTACTTCT ACTGAAGGAA GTGGCACTAT GAAGAAGCTG 
GATCCAGCCG TGGGCTCTCC CCCGGCTCCT CCTCCTCCTC CTGTTCCCTC TCCTCCATTC 
CCTTGTCCTG CAGACAGGTC TCCTTTCCTT CCTCCCCCAC CTCCTGTCAC AGATTGCTCC 
CAGGGCTCTC CTCTGCCTCA CTCTCCTGTG TTCCCCCCTC CGCCGCCAGA AGCTCTCATT 
CCTTTCTGCT CCCCACCTGA TTGGTGCCTT TCTCCTCCCC GCCCTGCACT GAGCCCCATT 
CTTCCAGATT CACCTGTGTC CTTGCCATTG CCCCCACCTC TCTTACCTTC CTCGGAACCC 
CCACCTGCCC CACCTCTTGA CCCCAAATTC ATGAAAGACA CCAGGCCGCC TTTCACAAAT 
TCTGGCCAGC CAGAATCCTC CCGGGGATCC TTGAGGCCGC CTTCTACCAA GGAGGAGACC 
AGCAGGCCCC CCATGCCCCT GATAACCACG GAAGCATTGC AGATGGTGCA GTTGAGGCCC 
GTGAGAAAGA ACTCAGGCGC TGAGGCGGCA CAGTTGTCTG AACGAACAGC TCAGGAACAA 
CGAACTCCAG TTGCTCCACA GTACCACTTA AAGCCATCTG CTTTCCTGAA ATCCCGAAAT 
AGCACAAATG AAATGGAGAG TGAAAGCCAG CCTGCCTCTG TGACAAGCTC GCTTCCGACG 
CCTGCCAAGA GCTCGAGTCA GGGTGACCAT GGCAGTGCGG CTGAGCGTGG TGGCCCTGTG 
AGCCGCAGCC CTGGAGCTCC AAGCGCTGGG GAGGCAGAGG CTCGGCCCAG CCCCAGCACC 
ACCCCACTCC CAGACTCTTC ACCCAGCAGG AAGCCACCCC CCATTTCCAA GAAGCCCAAA 
CTGTTCCTGG TGGTACCACC TCCGCAGAAA GATTTTGCAG TGGAGCCCGC AGAGAACGTG 
AGCGAAGCCC TCCGAGCTGT GCCCAGCCCC ACGACGGGAG AGGAGGGCTC TGTGCACAGC 
AGGGAGGCAA AAGAGAGTTC TGCAGCCCAA GCTGGCTCTC ATGCCACGCA CCCTGGCACC 
TCGGTTCTTG AGGGAGGAGC TGCAGGATCC ATGTCTCCCA GCAGAGTGGA AGCCAATGTC 
CCCATGGTTC AGCCTGATGT CTCACCAGCC CCCAAGCAGG AGGAGCCAGC CGAGAACAGT 
GCGGATACTG GGGGCGATGG GGAGAGCTGC CTATCTCAAC AGGACGGAGC AGCTGGGGTG 
CCGGAGACCA ACGCAGCCGG TTCATCCTCA GAGGCCTGTG ACTTCCTCAA GGAAGACGGG 
AATGATGAGG TAATGACCCC CAGTCGACCC AGGACCACAG AAGACCTTTT TGCAGCTATT 
CACAGATCCA AAAGGAAAGT CCTCGGCCGT AGAGATTCAG ATGATGACCA CTCCCGAAAC 
CATTCTCCCT CCCCGCCCGT GACACCCACC GGCGCTGCCC CAAGCCTGGC CTCTCCAAAG 
CAAGTGGGGT CGATTCAGAG AAGCATCCGA AAGAGCAGCA CCAGCAGTGA CAACTTCAAA 
GCTCTGCTGC TGAAAAAGGG CAGTCGTTCA GACACCAGCG CCCGCATGTC TGCAGCAGAG 
ATGCTCAAGA ACACAGACCC TAGATTCCAG AGGTCGAGGT CAGAGCCTTC CCCAGATGCC 
CCCGAGAGCC CGTCAAGCTG CTCCCCAAGC AAGAACAGAA GGGCGCAGGA GGAGTGGGCC 
AAGAACGAAG GCTTGATGCC TCGGAGTCTG TCCTTTTCCG GCCCCAGGTA CGGCCGCAGC 
CGAACGCCGC CTTCTGCCGC CAGCAGCAGG TACAGCATGC GGAACCGGAT CCAGAGCAGC 
CCCATGACCG TCATCTCGGA GGGAGAAGGG GAAGCCGTGG AGCCTGTGGA CAGCATAGCC 
CGCGGGGCTC TGGGCGCTGC GGAGGGATGT TCCCTGGACG GACTGGCGAG GGAGGAGATG 
GACGAGGGCG GCCTGCTCTG TGGGGAGGGG CCTGCCGCCT CCCTGCAGCC CCAGGCCCCC 
GGCCCTGTGG ATGGGACAGC CAGTGCAGAG GGCAGAGAGC CCTCCCCACA GTGTGGCGGT 
TCTCTGAGCG AGGAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( NM_001144060.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137532.1 Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15007
Clone ID Related Accession (Same CDS sequence)	NM_001144060.1 , NM_001144060.2
Accession Version	NM_001144060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591375.8 and AL391669.11. On Jun 2, 2019 this sequence version replaced NM_001144060.1. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct start codon, and includes an alternate in-frame exon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus and includes an alternate segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGTGGTCT TCATTAATGC AAAGATTAAG TCTTTAATTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACC TAGATGAGGA AAGCCGATGG ACAGTCCACT ACACTGCCCC GTGGCACCAG 
CAGGAGAATG TGTTCCTTCC CACCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTACTGAGG GAATGCGATA AGTTGCGGCA TGATGGCTAC 
CGCAGTTCTC AGTACTACTC TCAGGGACCC ACCTTTGCGG CCAACGCCAG CCCATTCTGT 
GATGATTACC AAGATGAAGA TGAAGAAACA GATCAAAAGT GTTCTCTGTC TTCATCAGAA 
GAAGAAAGAT TTATTTCCAT CAGGAGACCT AAAACACCAG CCTCAAGTGA CTTCTCCGAC 
CTTAATACTC AGACGAACTG GACCAAGTCG CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CCCAAACAGT CCAGGCTGAC GTGGTGCCTA TTAACATAAC TGGGGAGAAT 
TTCGATCGCC AGGCCAGCCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA 
CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG 
CTAGCATCAG GCACTGGCCA AGATGATGCT GATGGCCACT CAGTGTACAC CCCTGATCAC 
TACTCTACAC TAGGAAGGTT CAATAGCTGT CGGTCTGCTG GGCAGCGCTC AGAAACCAGG 
GACTCCAGCT GTCAGACGGA GGATGTGAAG GTCGTACCAC CTTCCATGAG GAGAATCAGG 
GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGGGCCACT TCTCAGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGATTC TGCAGGGATC GTATTCCCTT CCCGCCTTGA CAGTGATGCT 
GGCTTCCATA GTCTTCCGCG TTCTGGAGCA AGGGCAAACA TTCAGTCCCT TGAGCCGAGG 
CTGGGTGCCC TCGGCCCTGC AGGAGACATG AATGGCACTT TCCTCTACCA GAGAGGTCAC 
CCACAAGCAG ATGAAAACTT AGGCCATTTA GGAGGTGCCT CAGGGACTGG AACACTTTTG 
AGACCCAAAT CCCAGGAGTT GAGACACTTC GAGAGTGAAA ATATAATGAG CCCAGCGTGT 
GTGGTTTCTC CTCATGCAAC CTACTCCACC AGCATCATCC CAAATGCCAC ACTGTCTTCC 
TCTTCCGAGG TCATCGCTAT TCCCACTGCT CAGAGTGCGG GACAGCGGGA AAGTAAAAGT 
TCTGGCTCAT CACATGCAAG GATAAAATCC AGAGACCACC TCATCTCCAG GCATGCTGTG 
AAAGGTGATC CTCAGTCTCC CGGTCGCCAC TGGAATGAGG GCCATGCCAC CATTCTTTCA 
CAGGACTTAG ACCCTCATTC CCCTGGTGAA CCCGCACTGT TGTCCCTCTG TGACTCAGCC 
GTCCCTCTAA ATGCTCCAGC AAATAGGGAG AATGGGTCCC AAGCTATGCC GTATAATTGT 
AGAAACAACC TGGCCTTCCC AGCCCACCCC CAAGATGTGG ATGGCAAGAG TGAATCTAGT 
TATTCAGGGG GCGGAGGGCA CAGCAGCTCG GAGCCCTGGG AATACAAATC CTCAGGTAAT 
GGAAGGGCAT CCCCCCTGAA GCCGCATTTA GCAACTCCTG GCTATTCCAC TCCCACAAGT 
AACATGAGCA GCTGCAGTTT GGACCAAACG TCCAACAAAG AGGATGCTGG GTCGCTGTAT 
TCTGAGGACC ACGATGGCTA CTGTGCATCT GTGCACACTG ACTCTGGACA TGGATCTGGG 
AATCTGTGCA ATAGCAGTGA TGGCTTTGGG AACCCCAGGC ACAGCGTGAT CAATGTTTTT 
GTTGGAAGAG CTCAGAAAAA CCAAGGGGAC CGGTCCAATT ACCAGGATAA ATCCCTATCA 
AGAAACATCT CTTTGAAGAA AGCAAAGAAG CCTCCCCTGC CACCCTCCCG GACAGACTCC 
CTCCGCAGGA TTCCCAAGAA GAGCAGCCAG TGCAACGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCACAC TCCAGCACTC GCTGCAGCTG AGCCTCCCAG GCAAGAGTGG CAGCTCGCCC 
TCCCAGAGCC CCTGCAGTGA CTTGGAAGAG CCCTGGCTGC CCCGCTCCCG GAGCCAGAGC 
ACAGTTAGTG CTGGCAGCAG CATGACTTCC GCCACCACCC CCAATGTCTA CTCCCTGTGC 
GGGGCCACGC CATCGCAGAG TGACACAAGC AGCGTCAAGT CAGAGTACAC GGACCCCTGG 
GGTTATTACA TTGACTACAC GGGCATGCAG GAAGATCCGG GGAACCCGGC AGGGGGCTGT 
TCAACCAGCA GTGGGGTGCC CACTGGGAAC GGGCCAGTCC GCCATGTCCA AGAAGGGTCC 
AGAGCCACAA TGCCCCAAGT GCCCGGTGGT TCAGTCAAAC CAAAGATCAT GTCACCAGAG 
AAGTCACACA GAGTCATTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA 
GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAACGG GAAGGGGAAG 
CCCAAGCCCA AGGTACCAGA AAGGAAGTCC TCTCTGATAT CTTCAGTATC CATTTCCTCA 
TCGTCCACTT CTCTTTCTTC TAGTACTTCT ACTGAAGGAA GTGGCACTAT GAAGAAGCTG 
GATCCAGCCG TGGGCTCTCC CCCGGCTCCT CCTCCTCCTC CTGTTCCCTC TCCTCCATTC 
CCTTGTCCTG CAGACAGGTC TCCTTTCCTT CCTCCCCCAC CTCCTGTCAC AGATTGCTCC 
CAGGGCTCTC CTCTGCCTCA CTCTCCTGTG TTCCCCCCTC CGCCGCCAGA AGCTCTCATT 
CCTTTCTGCT CCCCACCTGA TTGGTGCCTT TCTCCTCCCC GCCCTGCACT GAGCCCCATT 
CTTCCAGATT CACCTGTGTC CTTGCCATTG CCCCCACCTC TCTTACCTTC CTCGGAACCC 
CCACCTGCCC CACCTCTTGA CCCCAAATTC ATGAAAGACA CCAGGCCGCC TTTCACAAAT 
TCTGGCCAGC CAGAATCCTC CCGGGGATCC TTGAGGCCGC CTTCTACCAA GGAGGAGACC 
AGCAGGCCCC CCATGCCCCT GATAACCACG GAAGCATTGC AGATGGTGCA GTTGAGGCCC 
GTGAGAAAGA ACTCAGGCGC TGAGGCGGCA CAGTTGTCTG AACGAACAGC TCAGGAACAA 
CGAACTCCAG TTGCTCCACA GTACCACTTA AAGCCATCTG CTTTCCTGAA ATCCCGAAAT 
AGCACAAATG AAATGGAGAG TGAAAGCCAG CCTGCCTCTG TGACAAGCTC GCTTCCGACG 
CCTGCCAAGA GCTCGAGTCA GGGTGACCAT GGCAGTGCGG CTGAGCGTGG TGGCCCTGTG 
AGCCGCAGCC CTGGAGCTCC AAGCGCTGGG GAGGCAGAGG CTCGGCCCAG CCCCAGCACC 
ACCCCACTCC CAGACTCTTC ACCCAGCAGG AAGCCACCCC CCATTTCCAA GAAGCCCAAA 
CTGTTCCTGG TGGTACCACC TCCGCAGAAA GATTTTGCAG TGGAGCCCGC AGAGAACGTG 
AGCGAAGCCC TCCGAGCTGT GCCCAGCCCC ACGACGGGAG AGGAGGGCTC TGTGCACAGC 
AGGGAGGCAA AAGAGAGTTC TGCAGCCCAA GCTGGCTCTC ATGCCACGCA CCCTGGCACC 
TCGGTTCTTG AGGGAGGAGC TGCAGGATCC ATGTCTCCCA GCAGAGTGGA AGCCAATGTC 
CCCATGGTTC AGCCTGATGT CTCACCAGCC CCCAAGCAGG AGGAGCCAGC CGAGAACAGT 
GCGGATACTG GGGGCGATGG GGAGAGCTGC CTATCTCAAC AGGACGGAGC AGCTGGGGTG 
CCGGAGACCA ACGCAGCCGG TTCATCCTCA GAGGCCTGTG ACTTCCTCAA GGAAGACGGG 
AATGATGAGG TAATGACCCC CAGTCGACCC AGGACCACAG AAGACCTTTT TGCAGCTATT 
CACAGATCCA AAAGGAAAGT CCTCGGCCGT AGAGATTCAG ATGATGACCA CTCCCGAAAC 
CATTCTCCCT CCCCGCCCGT GACACCCACC GGCGCTGCCC CAAGCCTGGC CTCTCCAAAG 
CAAGTGGGGT CGATTCAGAG AAGCATCCGA AAGAGCAGCA CCAGCAGTGA CAACTTCAAA 
GCTCTGCTGC TGAAAAAGGG CAGTCGTTCA GACACCAGCG CCCGCATGTC TGCAGCAGAG 
ATGCTCAAGA ACACAGACCC TAGATTCCAG AGGTCGAGGT CAGAGCCTTC CCCAGATGCC 
CCCGAGAGCC CGTCAAGCTG CTCCCCAAGC AAGAACAGAA GGGCGCAGGA GGAGTGGGCC 
AAGAACGAAG GCTTGATGCC TCGGAGTCTG TCCTTTTCCG GCCCCAGGTA CGGCCGCAGC 
CGAACGCCGC CTTCTGCCGC CAGCAGCAGG TACAGCATGC GGAACCGGAT CCAGAGCAGC 
CCCATGACCG TCATCTCGGA GGGAGAAGGG GAAGCCGTGG AGCCTGTGGA CAGCATAGCC 
CGCGGGGCTC TGGGCGCTGC GGAGGGATGT TCCCTGGACG GACTGGCGAG GGAGGAGATG 
GACGAGGGCG GCCTGCTCTG TGGGGAGGGG CCTGCCGCCT CCCTGCAGCC CCAGGCCCCC 
GGCCCTGTGG ATGGGACAGC CAGTGCAGAG GGCAGAGAGC CCTCCCCACA GTGTGGCGGT 
TCTCTGAGCG AGGAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137532.1
CDS	205..5025
Translation	MVVFINAKIKSLIKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	NM_001144060.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144060.2

ORF Insert Sequence:

ATGGTGGTCT TCATTAATGC AAAGATTAAG TCTTTAATTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACC TAGATGAGGA AAGCCGATGG ACAGTCCACT ACACTGCCCC GTGGCACCAG 
CAGGAGAATG TGTTCCTTCC CACCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTACTGAGG GAATGCGATA AGTTGCGGCA TGATGGCTAC 
CGCAGTTCTC AGTACTACTC TCAGGGACCC ACCTTTGCGG CCAACGCCAG CCCATTCTGT 
GATGATTACC AAGATGAAGA TGAAGAAACA GATCAAAAGT GTTCTCTGTC TTCATCAGAA 
GAAGAAAGAT TTATTTCCAT CAGGAGACCT AAAACACCAG CCTCAAGTGA CTTCTCCGAC 
CTTAATACTC AGACGAACTG GACCAAGTCG CTTCCACTGC CAACACCAGA AGAGAAGATG 
CGACAGCAAG CCCAAACAGT CCAGGCTGAC GTGGTGCCTA TTAACATAAC TGGGGAGAAT 
TTCGATCGCC AGGCCAGCCT TCGGCGGTCT CTAATTTACA CAGACACTCT GGTAAGACGA 
CCGAAGAAAG TCAAAAGGAG AAAGACTATT ACAGGAGTCC CTGACAACAT ACAGAAGGAG 
CTAGCATCAG GCACTGGCCA AGATGATGCT GATGGCCACT CAGTGTACAC CCCTGATCAC 
TACTCTACAC TAGGAAGGTT CAATAGCTGT CGGTCTGCTG GGCAGCGCTC AGAAACCAGG 
GACTCCAGCT GTCAGACGGA GGATGTGAAG GTCGTACCAC CTTCCATGAG GAGAATCAGG 
GCACAGAAGG GGCAAGGCAT TGCTGCCCAG ATGGGCCACT TCTCAGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGATTC TGCAGGGATC GTATTCCCTT CCCGCCTTGA CAGTGATGCT 
GGCTTCCATA GTCTTCCGCG TTCTGGAGCA AGGGCAAACA TTCAGTCCCT TGAGCCGAGG 
CTGGGTGCCC TCGGCCCTGC AGGAGACATG AATGGCACTT TCCTCTACCA GAGAGGTCAC 
CCACAAGCAG ATGAAAACTT AGGCCATTTA GGAGGTGCCT CAGGGACTGG AACACTTTTG 
AGACCCAAAT CCCAGGAGTT GAGACACTTC GAGAGTGAAA ATATAATGAG CCCAGCGTGT 
GTGGTTTCTC CTCATGCAAC CTACTCCACC AGCATCATCC CAAATGCCAC ACTGTCTTCC 
TCTTCCGAGG TCATCGCTAT TCCCACTGCT CAGAGTGCGG GACAGCGGGA AAGTAAAAGT 
TCTGGCTCAT CACATGCAAG GATAAAATCC AGAGACCACC TCATCTCCAG GCATGCTGTG 
AAAGGTGATC CTCAGTCTCC CGGTCGCCAC TGGAATGAGG GCCATGCCAC CATTCTTTCA 
CAGGACTTAG ACCCTCATTC CCCTGGTGAA CCCGCACTGT TGTCCCTCTG TGACTCAGCC 
GTCCCTCTAA ATGCTCCAGC AAATAGGGAG AATGGGTCCC AAGCTATGCC GTATAATTGT 
AGAAACAACC TGGCCTTCCC AGCCCACCCC CAAGATGTGG ATGGCAAGAG TGAATCTAGT 
TATTCAGGGG GCGGAGGGCA CAGCAGCTCG GAGCCCTGGG AATACAAATC CTCAGGTAAT 
GGAAGGGCAT CCCCCCTGAA GCCGCATTTA GCAACTCCTG GCTATTCCAC TCCCACAAGT 
AACATGAGCA GCTGCAGTTT GGACCAAACG TCCAACAAAG AGGATGCTGG GTCGCTGTAT 
TCTGAGGACC ACGATGGCTA CTGTGCATCT GTGCACACTG ACTCTGGACA TGGATCTGGG 
AATCTGTGCA ATAGCAGTGA TGGCTTTGGG AACCCCAGGC ACAGCGTGAT CAATGTTTTT 
GTTGGAAGAG CTCAGAAAAA CCAAGGGGAC CGGTCCAATT ACCAGGATAA ATCCCTATCA 
AGAAACATCT CTTTGAAGAA AGCAAAGAAG CCTCCCCTGC CACCCTCCCG GACAGACTCC 
CTCCGCAGGA TTCCCAAGAA GAGCAGCCAG TGCAACGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCACAC TCCAGCACTC GCTGCAGCTG AGCCTCCCAG GCAAGAGTGG CAGCTCGCCC 
TCCCAGAGCC CCTGCAGTGA CTTGGAAGAG CCCTGGCTGC CCCGCTCCCG GAGCCAGAGC 
ACAGTTAGTG CTGGCAGCAG CATGACTTCC GCCACCACCC CCAATGTCTA CTCCCTGTGC 
GGGGCCACGC CATCGCAGAG TGACACAAGC AGCGTCAAGT CAGAGTACAC GGACCCCTGG 
GGTTATTACA TTGACTACAC GGGCATGCAG GAAGATCCGG GGAACCCGGC AGGGGGCTGT 
TCAACCAGCA GTGGGGTGCC CACTGGGAAC GGGCCAGTCC GCCATGTCCA AGAAGGGTCC 
AGAGCCACAA TGCCCCAAGT GCCCGGTGGT TCAGTCAAAC CAAAGATCAT GTCACCAGAG 
AAGTCACACA GAGTCATTTC TCCATCCAGT GGGTATTCCA GCCAGTCGAA TACACCCACA 
GCACTCACCC CTGTGCCTGT GTTTTTAAAA TCAGTGTCAC CAGCAAACGG GAAGGGGAAG 
CCCAAGCCCA AGGTACCAGA AAGGAAGTCC TCTCTGATAT CTTCAGTATC CATTTCCTCA 
TCGTCCACTT CTCTTTCTTC TAGTACTTCT ACTGAAGGAA GTGGCACTAT GAAGAAGCTG 
GATCCAGCCG TGGGCTCTCC CCCGGCTCCT CCTCCTCCTC CTGTTCCCTC TCCTCCATTC 
CCTTGTCCTG CAGACAGGTC TCCTTTCCTT CCTCCCCCAC CTCCTGTCAC AGATTGCTCC 
CAGGGCTCTC CTCTGCCTCA CTCTCCTGTG TTCCCCCCTC CGCCGCCAGA AGCTCTCATT 
CCTTTCTGCT CCCCACCTGA TTGGTGCCTT TCTCCTCCCC GCCCTGCACT GAGCCCCATT 
CTTCCAGATT CACCTGTGTC CTTGCCATTG CCCCCACCTC TCTTACCTTC CTCGGAACCC 
CCACCTGCCC CACCTCTTGA CCCCAAATTC ATGAAAGACA CCAGGCCGCC TTTCACAAAT 
TCTGGCCAGC CAGAATCCTC CCGGGGATCC TTGAGGCCGC CTTCTACCAA GGAGGAGACC 
AGCAGGCCCC CCATGCCCCT GATAACCACG GAAGCATTGC AGATGGTGCA GTTGAGGCCC 
GTGAGAAAGA ACTCAGGCGC TGAGGCGGCA CAGTTGTCTG AACGAACAGC TCAGGAACAA 
CGAACTCCAG TTGCTCCACA GTACCACTTA AAGCCATCTG CTTTCCTGAA ATCCCGAAAT 
AGCACAAATG AAATGGAGAG TGAAAGCCAG CCTGCCTCTG TGACAAGCTC GCTTCCGACG 
CCTGCCAAGA GCTCGAGTCA GGGTGACCAT GGCAGTGCGG CTGAGCGTGG TGGCCCTGTG 
AGCCGCAGCC CTGGAGCTCC AAGCGCTGGG GAGGCAGAGG CTCGGCCCAG CCCCAGCACC 
ACCCCACTCC CAGACTCTTC ACCCAGCAGG AAGCCACCCC CCATTTCCAA GAAGCCCAAA 
CTGTTCCTGG TGGTACCACC TCCGCAGAAA GATTTTGCAG TGGAGCCCGC AGAGAACGTG 
AGCGAAGCCC TCCGAGCTGT GCCCAGCCCC ACGACGGGAG AGGAGGGCTC TGTGCACAGC 
AGGGAGGCAA AAGAGAGTTC TGCAGCCCAA GCTGGCTCTC ATGCCACGCA CCCTGGCACC 
TCGGTTCTTG AGGGAGGAGC TGCAGGATCC ATGTCTCCCA GCAGAGTGGA AGCCAATGTC 
CCCATGGTTC AGCCTGATGT CTCACCAGCC CCCAAGCAGG AGGAGCCAGC CGAGAACAGT 
GCGGATACTG GGGGCGATGG GGAGAGCTGC CTATCTCAAC AGGACGGAGC AGCTGGGGTG 
CCGGAGACCA ACGCAGCCGG TTCATCCTCA GAGGCCTGTG ACTTCCTCAA GGAAGACGGG 
AATGATGAGG TAATGACCCC CAGTCGACCC AGGACCACAG AAGACCTTTT TGCAGCTATT 
CACAGATCCA AAAGGAAAGT CCTCGGCCGT AGAGATTCAG ATGATGACCA CTCCCGAAAC 
CATTCTCCCT CCCCGCCCGT GACACCCACC GGCGCTGCCC CAAGCCTGGC CTCTCCAAAG 
CAAGTGGGGT CGATTCAGAG AAGCATCCGA AAGAGCAGCA CCAGCAGTGA CAACTTCAAA 
GCTCTGCTGC TGAAAAAGGG CAGTCGTTCA GACACCAGCG CCCGCATGTC TGCAGCAGAG 
ATGCTCAAGA ACACAGACCC TAGATTCCAG AGGTCGAGGT CAGAGCCTTC CCCAGATGCC 
CCCGAGAGCC CGTCAAGCTG CTCCCCAAGC AAGAACAGAA GGGCGCAGGA GGAGTGGGCC 
AAGAACGAAG GCTTGATGCC TCGGAGTCTG TCCTTTTCCG GCCCCAGGTA CGGCCGCAGC 
CGAACGCCGC CTTCTGCCGC CAGCAGCAGG TACAGCATGC GGAACCGGAT CCAGAGCAGC 
CCCATGACCG TCATCTCGGA GGGAGAAGGG GAAGCCGTGG AGCCTGTGGA CAGCATAGCC 
CGCGGGGCTC TGGGCGCTGC GGAGGGATGT TCCCTGGACG GACTGGCGAG GGAGGAGATG 
GACGAGGGCG GCCTGCTCTG TGGGGAGGGG CCTGCCGCCT CCCTGCAGCC CCAGGCCCCC 
GGCCCTGTGG ATGGGACAGC CAGTGCAGAG GGCAGAGAGC CCTCCCCACA GTGTGGCGGT 
TCTCTGAGCG AGGAGAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_005267062.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267119.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X5, mRNA.

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CloneID	OHu40882
Clone ID Related Accession (Same CDS sequence)	XM_005267062.4 , XM_005267062.5
Accession Version	XM_005267062.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267062.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT 
TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA 
GTCCCTGACA ACATACAGAA GGAGCTAGCA TCAGGCACTG GCCAAGATGA TGCTGATGGC 
CACTCAGTGT ACACCCCTGA TCACTACTCT ACACTAGGAA GGTTCAATAG CTGTCGGTCT 
GCTGGGCAGC GCTCAGAAAC CAGGGACTCC AGCTGTCAGA CGGAGGATGT GAAGGTCGTA 
CCACCTTCCA TGAGGAGAAT CAGGGCACAG AAGGGGCAAG GCATTGCTGC CCAGATGGGC 
CACTTCTCAG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ATTCTGCAGG GATCGTATTC 
CCTTCCCGCC TTGACAGTGA TGCTGGCTTC CATAGTCTTC CGCGTTCTGG AGCAAGGGCA 
AACATTCAGT CCCTTGAGCC GAGGCTGGGT GCCCTCGGCC CTGCAGGAGA CATGAATGGC 
ACTTTCCTCT ACCAGAGAGG TCACCCACAA GCAGATGAAA ACTTAGGCCA TTTAGGAGGT 
GCCTCAGGGA CTGGAACACT TTTGAGACCC AAATCCCAGG AGTTGAGACA CTTCGAGAGT 
GAAAATATAA TGAGCCCAGC GTGTGTGGTT TCTCCTCATG CAACCTACTC CACCAGCATC 
ATCCCAAATG CCACACTGTC TTCCTCTTCC GAGGTCATCG CTATTCCCAC TGCTCAGAGT 
GCGGGACAGC GGGAAAGTAA AAGTTCTGGC TCATCACATG CAAGGATAAA ATCCAGAGAC 
CACCTCATCT CCAGGCATGC TGTGAAAGGT GATCCTCAGT CTCCCGGTCG CCACTGGAAT 
GAGGGCCATG CCACCATTCT TTCACAGGAC TTAGACCCTC ATTCCCCTGG TGAACCCGCA 
CTGTTGTCCC TCTGTGACTC AGCCGTCCCT CTAAATGCTC CAGCAAATAG GGAGAATGGG 
TCCCAAGCTA TGCCGTATAA TTGTAGAAAC AACCTGGCCT TCCCAGCCCA CCCCCAAGAT 
GTGGATGGCA AGAGTGAATC TAGTTATTCA GGGGGCGGAG GGCACAGCAG CTCGGAGCCC 
TGGGAATACA AATCCTCAGG TAATGGAAGG GCATCCCCCC TGAAGCCGCA TTTAGCAACT 
CCTGGCTATT CCACTCCCAC AAGTAACATG AGCAGCTGCA GTTTGGACCA AACGTCCAAC 
AAAGAGGATG CTGGGTCGCT GTATTCTGAG GACCACGATG GCTACTGTGC ATCTGTGCAC 
ACTGACTCTG GACATGGATC TGGGAATCTG TGCAATAGCA GTGATGGCTT TGGGAACCCC 
AGGCACAGCG TGATCAATGT TTTTGTTGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCC 
AATTACCAGG ATAAATCCCT ATCAAGAAAC ATCTCTTTGA AGAAAGCAAA GAAGCCTCCC 
CTGCCACCCT CCCGGACAGA CTCCCTCCGC AGGATTCCCA AGAAGAGCAG CCAGTGCAAC 
GGGCAGGTGC TCAACGAGAG CCTGATCGCC ACACTCCAGC ACTCGCTGCA GCTGAGCCTC 
CCAGGCAAGA GTGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGACTTGGA AGAGCCCTGG 
CTGCCCCGCT CCCGGAGCCA GAGCACAGTT AGTGCTGGCA GCAGCATGAC TTCCGCCACC 
ACCCCCAATG TCTACTCCCT GTGCGGGGCC ACGCCATCGC AGAGTGACAC AAGCAGCGTC 
AAGTCAGAGT ACACGGACCC CTGGGGTTAT TACATTGACT ACACGGGCAT GCAGGAAGAT 
CCGGGGAACC CGGCAGGGGG CTGTTCAACC AGCAGTGGGG TGCCCACTGG GAACGGGCCA 
GTCCGCCATG TCCAAGAAGG GTCCAGAGCC ACAATGCCCC AAGTGCCCGG TGGTTCAGTC 
AAACCAAAGA TCATGTCACC AGAGAAGTCA CACAGAGTCA TTTCTCCATC CAGTGGGTAT 
TCCAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAGTG 
TCACCAGCAA ACGGGAAGGG GAAGCCCAAG CCCAAGGTAC CAGAAAGGAA GTCCTCTCTG 
ATATCTTCAG TATCCATTTC CTCATCGTCC ACTTCTCTTT CTTCTAGTAC TTCTACTGAA 
GGAAGTGGCA CTATGAAGAA GCTGGATCCA GCCGTGGGCT CTCCCCCGGC TCCTCCTCCT 
CCTCCTGTTC CCTCTCCTCC ATTCCCTTGT CCTGCAGACA GGTCTCCTTT CCTTCCTCCC 
CCACCTCCTG TCACAGATTG CTCCCAGGGC TCTCCTCTGC CTCACTCTCC TGTGTTCCCC 
CCTCCGCCGC CAGAAGCTCT CATTCCTTTC TGCTCCCCAC CTGATTGGTG CCTTTCTCCT 
CCCCGCCCTG CACTGAGCCC CATTCTTCCA GATTCACCTG TGTCCTTGCC ATTGCCCCCA 
CCTCTCTTAC CTTCCTCGGA ACCCCCACCT GCCCCACCTC TTGACCCCAA ATTCATGAAA 
GACACCAGGC CGCCTTTCAC AAATTCTGGC CAGCCAGAAT CCTCCCGGGG ATCCTTGAGG 
CCGCCTTCTA CCAAGGAGGA GACCAGCAGG CCCCCCATGC CCCTGATAAC CACGGAAGCA 
TTGCAGATGG TGCAGTTGAG GCCCGTGAGA AAGAACTCAG GCGCTGAGGC GGCACAGTTG 
TCTGAACGAA CAGCTCAGGA ACAACGAACT CCAGTTGCTC CACAGTACCA CTTAAAGCCA 
TCTGCTTTCC TGAAATCCCG AAATAGCACA AATGAAATGG AGAGTGAAAG CCAGCCTGCC 
TCTGTGACAA GCTCGCTTCC GACGCCTGCC AAGAGCTCGA GTCAGGGTGA CCATGGCAGT 
GCGGCTGAGC GTGGTGGCCC TGTGAGCCGC AGCCCTGGAG CTCCAAGCGC TGGGGAGGCA 
GAGGCTCGGC CCAGCCCCAG CACCACCCCA CTCCCAGACT CTTCACCCAG CAGGAAGCCA 
CCCCCCATTT CCAAGAAGCC CAAACTGTTC CTGGTGGTAC CACCTCCGCA GAAAGATTTT 
GCAGTGGAGC CCGCAGAGAA CGTGAGCGAA GCCCTCCGAG CTGTGCCCAG CCCCACGACG 
GGAGAGGAGG GCTCTGTGCA CAGCAGGGAG GCAAAAGAGA GTTCTGCAGC CCAAGCTGGC 
TCTCATGCCA CGCACCCTGG CACCTCGGTT CTTGAGGGAG GAGCTGCAGG ATCCATGTCT 
CCCAGCAGAG TGGAAGCCAA TGTCCCCATG GTTCAGCCTG ATGTCTCACC AGCCCCCAAG 
CAGGAGGAGC CAGCCGAGAA CAGTGCGGAT ACTGGGGGCG ATGGGGAGAG CTGCCTATCT 
CAACAGGACG GAGCAGCTGG GGTGCCGGAG ACCAACGCAG CCGGTTCATC CTCAGAGGCC 
TGTGACTTCC TCAAGGAAGA CGGGAATGAT GAGGTAATGA CCCCCAGTCG ACCCAGGACC 
ACAGAAGACC TTTTTGCAGC TATTCACAGA TCCAAAAGGA AAGTCCTCGG CCGTAGAGAT 
TCAGATGATG ACCACTCCCG AAACCATTCT CCCTCCCCGC CCGTGACACC CACCGGCGCT 
GCCCCAAGCC TGGCCTCTCC AAAGCAAGTG GGGTCGATTC AGAGAAGCAT CCGAAAGAGC 
AGCACCAGCA GTGACAACTT CAAAGCTCTG CTGCTGAAAA AGGGCAGTCG TTCAGACACC 
AGCGCCCGCA TGTCTGCAGC AGAGATGCTC AAGAACACAG ACCCTAGATT CCAGAGGTCG 
AGGTCAGAGC CTTCCCCAGA TGCCCCCGAG AGCCCGTCAA GCTGCTCCCC AAGCAAGAAC 
AGAAGGGCGC AGGAGGAGTG GGCCAAGAAC GAAGGCTTGA TGCCTCGGAG TCTGTCCTTT 
TCCGGCCCCA GGTACGGCCG CAGCCGAACG CCGCCTTCTG CCGCCAGCAG CAGGTACAGC 
ATGCGGAACC GGATCCAGAG CAGCCCCATG ACCGTCATCT CGGAGGGAGA AGGGGAAGCC 
GTGGAGCCTG TGGACAGCAT AGCCCGCGGG GCTCTGGGCG CTGCGGAGGG ATGTTCCCTG 
GACGGACTGG CGAGGGAGGA GATGGACGAG GGCGGCCTGC TCTGTGGGGA GGGGCCTGCC 
GCCTCCCTGC AGCCCCAGGC CCCCGGCCCT GTGGATGGGA CAGCCAGTGC AGAGGGCAGA 
GAGCCCTCCC CACAGTGTGG CGGTTCTCTG AGCGAGGAGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267119.1
CDS	2273..7237
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_005267062.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267062.4

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT 
TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA 
GTCCCTGACA ACATACAGAA GGAGCTAGCA TCAGGCACTG GCCAAGATGA TGCTGATGGC 
CACTCAGTGT ACACCCCTGA TCACTACTCT ACACTAGGAA GGTTCAATAG CTGTCGGTCT 
GCTGGGCAGC GCTCAGAAAC CAGGGACTCC AGCTGTCAGA CGGAGGATGT GAAGGTCGTA 
CCACCTTCCA TGAGGAGAAT CAGGGCACAG AAGGGGCAAG GCATTGCTGC CCAGATGGGC 
CACTTCTCAG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ATTCTGCAGG GATCGTATTC 
CCTTCCCGCC TTGACAGTGA TGCTGGCTTC CATAGTCTTC CGCGTTCTGG AGCAAGGGCA 
AACATTCAGT CCCTTGAGCC GAGGCTGGGT GCCCTCGGCC CTGCAGGAGA CATGAATGGC 
ACTTTCCTCT ACCAGAGAGG TCACCCACAA GCAGATGAAA ACTTAGGCCA TTTAGGAGGT 
GCCTCAGGGA CTGGAACACT TTTGAGACCC AAATCCCAGG AGTTGAGACA CTTCGAGAGT 
GAAAATATAA TGAGCCCAGC GTGTGTGGTT TCTCCTCATG CAACCTACTC CACCAGCATC 
ATCCCAAATG CCACACTGTC TTCCTCTTCC GAGGTCATCG CTATTCCCAC TGCTCAGAGT 
GCGGGACAGC GGGAAAGTAA AAGTTCTGGC TCATCACATG CAAGGATAAA ATCCAGAGAC 
CACCTCATCT CCAGGCATGC TGTGAAAGGT GATCCTCAGT CTCCCGGTCG CCACTGGAAT 
GAGGGCCATG CCACCATTCT TTCACAGGAC TTAGACCCTC ATTCCCCTGG TGAACCCGCA 
CTGTTGTCCC TCTGTGACTC AGCCGTCCCT CTAAATGCTC CAGCAAATAG GGAGAATGGG 
TCCCAAGCTA TGCCGTATAA TTGTAGAAAC AACCTGGCCT TCCCAGCCCA CCCCCAAGAT 
GTGGATGGCA AGAGTGAATC TAGTTATTCA GGGGGCGGAG GGCACAGCAG CTCGGAGCCC 
TGGGAATACA AATCCTCAGG TAATGGAAGG GCATCCCCCC TGAAGCCGCA TTTAGCAACT 
CCTGGCTATT CCACTCCCAC AAGTAACATG AGCAGCTGCA GTTTGGACCA AACGTCCAAC 
AAAGAGGATG CTGGGTCGCT GTATTCTGAG GACCACGATG GCTACTGTGC ATCTGTGCAC 
ACTGACTCTG GACATGGATC TGGGAATCTG TGCAATAGCA GTGATGGCTT TGGGAACCCC 
AGGCACAGCG TGATCAATGT TTTTGTTGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCC 
AATTACCAGG ATAAATCCCT ATCAAGAAAC ATCTCTTTGA AGAAAGCAAA GAAGCCTCCC 
CTGCCACCCT CCCGGACAGA CTCCCTCCGC AGGATTCCCA AGAAGAGCAG CCAGTGCAAC 
GGGCAGGTGC TCAACGAGAG CCTGATCGCC ACACTCCAGC ACTCGCTGCA GCTGAGCCTC 
CCAGGCAAGA GTGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGACTTGGA AGAGCCCTGG 
CTGCCCCGCT CCCGGAGCCA GAGCACAGTT AGTGCTGGCA GCAGCATGAC TTCCGCCACC 
ACCCCCAATG TCTACTCCCT GTGCGGGGCC ACGCCATCGC AGAGTGACAC AAGCAGCGTC 
AAGTCAGAGT ACACGGACCC CTGGGGTTAT TACATTGACT ACACGGGCAT GCAGGAAGAT 
CCGGGGAACC CGGCAGGGGG CTGTTCAACC AGCAGTGGGG TGCCCACTGG GAACGGGCCA 
GTCCGCCATG TCCAAGAAGG GTCCAGAGCC ACAATGCCCC AAGTGCCCGG TGGTTCAGTC 
AAACCAAAGA TCATGTCACC AGAGAAGTCA CACAGAGTCA TTTCTCCATC CAGTGGGTAT 
TCCAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAGTG 
TCACCAGCAA ACGGGAAGGG GAAGCCCAAG CCCAAGGTAC CAGAAAGGAA GTCCTCTCTG 
ATATCTTCAG TATCCATTTC CTCATCGTCC ACTTCTCTTT CTTCTAGTAC TTCTACTGAA 
GGAAGTGGCA CTATGAAGAA GCTGGATCCA GCCGTGGGCT CTCCCCCGGC TCCTCCTCCT 
CCTCCTGTTC CCTCTCCTCC ATTCCCTTGT CCTGCAGACA GGTCTCCTTT CCTTCCTCCC 
CCACCTCCTG TCACAGATTG CTCCCAGGGC TCTCCTCTGC CTCACTCTCC TGTGTTCCCC 
CCTCCGCCGC CAGAAGCTCT CATTCCTTTC TGCTCCCCAC CTGATTGGTG CCTTTCTCCT 
CCCCGCCCTG CACTGAGCCC CATTCTTCCA GATTCACCTG TGTCCTTGCC ATTGCCCCCA 
CCTCTCTTAC CTTCCTCGGA ACCCCCACCT GCCCCACCTC TTGACCCCAA ATTCATGAAA 
GACACCAGGC CGCCTTTCAC AAATTCTGGC CAGCCAGAAT CCTCCCGGGG ATCCTTGAGG 
CCGCCTTCTA CCAAGGAGGA GACCAGCAGG CCCCCCATGC CCCTGATAAC CACGGAAGCA 
TTGCAGATGG TGCAGTTGAG GCCCGTGAGA AAGAACTCAG GCGCTGAGGC GGCACAGTTG 
TCTGAACGAA CAGCTCAGGA ACAACGAACT CCAGTTGCTC CACAGTACCA CTTAAAGCCA 
TCTGCTTTCC TGAAATCCCG AAATAGCACA AATGAAATGG AGAGTGAAAG CCAGCCTGCC 
TCTGTGACAA GCTCGCTTCC GACGCCTGCC AAGAGCTCGA GTCAGGGTGA CCATGGCAGT 
GCGGCTGAGC GTGGTGGCCC TGTGAGCCGC AGCCCTGGAG CTCCAAGCGC TGGGGAGGCA 
GAGGCTCGGC CCAGCCCCAG CACCACCCCA CTCCCAGACT CTTCACCCAG CAGGAAGCCA 
CCCCCCATTT CCAAGAAGCC CAAACTGTTC CTGGTGGTAC CACCTCCGCA GAAAGATTTT 
GCAGTGGAGC CCGCAGAGAA CGTGAGCGAA GCCCTCCGAG CTGTGCCCAG CCCCACGACG 
GGAGAGGAGG GCTCTGTGCA CAGCAGGGAG GCAAAAGAGA GTTCTGCAGC CCAAGCTGGC 
TCTCATGCCA CGCACCCTGG CACCTCGGTT CTTGAGGGAG GAGCTGCAGG ATCCATGTCT 
CCCAGCAGAG TGGAAGCCAA TGTCCCCATG GTTCAGCCTG ATGTCTCACC AGCCCCCAAG 
CAGGAGGAGC CAGCCGAGAA CAGTGCGGAT ACTGGGGGCG ATGGGGAGAG CTGCCTATCT 
CAACAGGACG GAGCAGCTGG GGTGCCGGAG ACCAACGCAG CCGGTTCATC CTCAGAGGCC 
TGTGACTTCC TCAAGGAAGA CGGGAATGAT GAGGTAATGA CCCCCAGTCG ACCCAGGACC 
ACAGAAGACC TTTTTGCAGC TATTCACAGA TCCAAAAGGA AAGTCCTCGG CCGTAGAGAT 
TCAGATGATG ACCACTCCCG AAACCATTCT CCCTCCCCGC CCGTGACACC CACCGGCGCT 
GCCCCAAGCC TGGCCTCTCC AAAGCAAGTG GGGTCGATTC AGAGAAGCAT CCGAAAGAGC 
AGCACCAGCA GTGACAACTT CAAAGCTCTG CTGCTGAAAA AGGGCAGTCG TTCAGACACC 
AGCGCCCGCA TGTCTGCAGC AGAGATGCTC AAGAACACAG ACCCTAGATT CCAGAGGTCG 
AGGTCAGAGC CTTCCCCAGA TGCCCCCGAG AGCCCGTCAA GCTGCTCCCC AAGCAAGAAC 
AGAAGGGCGC AGGAGGAGTG GGCCAAGAAC GAAGGCTTGA TGCCTCGGAG TCTGTCCTTT 
TCCGGCCCCA GGTACGGCCG CAGCCGAACG CCGCCTTCTG CCGCCAGCAG CAGGTACAGC 
ATGCGGAACC GGATCCAGAG CAGCCCCATG ACCGTCATCT CGGAGGGAGA AGGGGAAGCC 
GTGGAGCCTG TGGACAGCAT AGCCCGCGGG GCTCTGGGCG CTGCGGAGGG ATGTTCCCTG 
GACGGACTGG CGAGGGAGGA GATGGACGAG GGCGGCCTGC TCTGTGGGGA GGGGCCTGCC 
GCCTCCCTGC AGCCCCAGGC CCCCGGCCCT GTGGATGGGA CAGCCAGTGC AGAGGGCAGA 
GAGCCCTCCC CACAGTGTGG CGGTTCTCTG AGCGAGGAGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_005267062.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005267119.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X4, mRNA.

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CloneID	OHu40882
Clone ID Related Accession (Same CDS sequence)	XM_005267062.4 , XM_005267062.5
Accession Version	XM_005267062.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005267062.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT 
TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA 
GTCCCTGACA ACATACAGAA GGAGCTAGCA TCAGGCACTG GCCAAGATGA TGCTGATGGC 
CACTCAGTGT ACACCCCTGA TCACTACTCT ACACTAGGAA GGTTCAATAG CTGTCGGTCT 
GCTGGGCAGC GCTCAGAAAC CAGGGACTCC AGCTGTCAGA CGGAGGATGT GAAGGTCGTA 
CCACCTTCCA TGAGGAGAAT CAGGGCACAG AAGGGGCAAG GCATTGCTGC CCAGATGGGC 
CACTTCTCAG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ATTCTGCAGG GATCGTATTC 
CCTTCCCGCC TTGACAGTGA TGCTGGCTTC CATAGTCTTC CGCGTTCTGG AGCAAGGGCA 
AACATTCAGT CCCTTGAGCC GAGGCTGGGT GCCCTCGGCC CTGCAGGAGA CATGAATGGC 
ACTTTCCTCT ACCAGAGAGG TCACCCACAA GCAGATGAAA ACTTAGGCCA TTTAGGAGGT 
GCCTCAGGGA CTGGAACACT TTTGAGACCC AAATCCCAGG AGTTGAGACA CTTCGAGAGT 
GAAAATATAA TGAGCCCAGC GTGTGTGGTT TCTCCTCATG CAACCTACTC CACCAGCATC 
ATCCCAAATG CCACACTGTC TTCCTCTTCC GAGGTCATCG CTATTCCCAC TGCTCAGAGT 
GCGGGACAGC GGGAAAGTAA AAGTTCTGGC TCATCACATG CAAGGATAAA ATCCAGAGAC 
CACCTCATCT CCAGGCATGC TGTGAAAGGT GATCCTCAGT CTCCCGGTCG CCACTGGAAT 
GAGGGCCATG CCACCATTCT TTCACAGGAC TTAGACCCTC ATTCCCCTGG TGAACCCGCA 
CTGTTGTCCC TCTGTGACTC AGCCGTCCCT CTAAATGCTC CAGCAAATAG GGAGAATGGG 
TCCCAAGCTA TGCCGTATAA TTGTAGAAAC AACCTGGCCT TCCCAGCCCA CCCCCAAGAT 
GTGGATGGCA AGAGTGAATC TAGTTATTCA GGGGGCGGAG GGCACAGCAG CTCGGAGCCC 
TGGGAATACA AATCCTCAGG TAATGGAAGG GCATCCCCCC TGAAGCCGCA TTTAGCAACT 
CCTGGCTATT CCACTCCCAC AAGTAACATG AGCAGCTGCA GTTTGGACCA AACGTCCAAC 
AAAGAGGATG CTGGGTCGCT GTATTCTGAG GACCACGATG GCTACTGTGC ATCTGTGCAC 
ACTGACTCTG GACATGGATC TGGGAATCTG TGCAATAGCA GTGATGGCTT TGGGAACCCC 
AGGCACAGCG TGATCAATGT TTTTGTTGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCC 
AATTACCAGG ATAAATCCCT ATCAAGAAAC ATCTCTTTGA AGAAAGCAAA GAAGCCTCCC 
CTGCCACCCT CCCGGACAGA CTCCCTCCGC AGGATTCCCA AGAAGAGCAG CCAGTGCAAC 
GGGCAGGTGC TCAACGAGAG CCTGATCGCC ACACTCCAGC ACTCGCTGCA GCTGAGCCTC 
CCAGGCAAGA GTGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGACTTGGA AGAGCCCTGG 
CTGCCCCGCT CCCGGAGCCA GAGCACAGTT AGTGCTGGCA GCAGCATGAC TTCCGCCACC 
ACCCCCAATG TCTACTCCCT GTGCGGGGCC ACGCCATCGC AGAGTGACAC AAGCAGCGTC 
AAGTCAGAGT ACACGGACCC CTGGGGTTAT TACATTGACT ACACGGGCAT GCAGGAAGAT 
CCGGGGAACC CGGCAGGGGG CTGTTCAACC AGCAGTGGGG TGCCCACTGG GAACGGGCCA 
GTCCGCCATG TCCAAGAAGG GTCCAGAGCC ACAATGCCCC AAGTGCCCGG TGGTTCAGTC 
AAACCAAAGA TCATGTCACC AGAGAAGTCA CACAGAGTCA TTTCTCCATC CAGTGGGTAT 
TCCAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAGTG 
TCACCAGCAA ACGGGAAGGG GAAGCCCAAG CCCAAGGTAC CAGAAAGGAA GTCCTCTCTG 
ATATCTTCAG TATCCATTTC CTCATCGTCC ACTTCTCTTT CTTCTAGTAC TTCTACTGAA 
GGAAGTGGCA CTATGAAGAA GCTGGATCCA GCCGTGGGCT CTCCCCCGGC TCCTCCTCCT 
CCTCCTGTTC CCTCTCCTCC ATTCCCTTGT CCTGCAGACA GGTCTCCTTT CCTTCCTCCC 
CCACCTCCTG TCACAGATTG CTCCCAGGGC TCTCCTCTGC CTCACTCTCC TGTGTTCCCC 
CCTCCGCCGC CAGAAGCTCT CATTCCTTTC TGCTCCCCAC CTGATTGGTG CCTTTCTCCT 
CCCCGCCCTG CACTGAGCCC CATTCTTCCA GATTCACCTG TGTCCTTGCC ATTGCCCCCA 
CCTCTCTTAC CTTCCTCGGA ACCCCCACCT GCCCCACCTC TTGACCCCAA ATTCATGAAA 
GACACCAGGC CGCCTTTCAC AAATTCTGGC CAGCCAGAAT CCTCCCGGGG ATCCTTGAGG 
CCGCCTTCTA CCAAGGAGGA GACCAGCAGG CCCCCCATGC CCCTGATAAC CACGGAAGCA 
TTGCAGATGG TGCAGTTGAG GCCCGTGAGA AAGAACTCAG GCGCTGAGGC GGCACAGTTG 
TCTGAACGAA CAGCTCAGGA ACAACGAACT CCAGTTGCTC CACAGTACCA CTTAAAGCCA 
TCTGCTTTCC TGAAATCCCG AAATAGCACA AATGAAATGG AGAGTGAAAG CCAGCCTGCC 
TCTGTGACAA GCTCGCTTCC GACGCCTGCC AAGAGCTCGA GTCAGGGTGA CCATGGCAGT 
GCGGCTGAGC GTGGTGGCCC TGTGAGCCGC AGCCCTGGAG CTCCAAGCGC TGGGGAGGCA 
GAGGCTCGGC CCAGCCCCAG CACCACCCCA CTCCCAGACT CTTCACCCAG CAGGAAGCCA 
CCCCCCATTT CCAAGAAGCC CAAACTGTTC CTGGTGGTAC CACCTCCGCA GAAAGATTTT 
GCAGTGGAGC CCGCAGAGAA CGTGAGCGAA GCCCTCCGAG CTGTGCCCAG CCCCACGACG 
GGAGAGGAGG GCTCTGTGCA CAGCAGGGAG GCAAAAGAGA GTTCTGCAGC CCAAGCTGGC 
TCTCATGCCA CGCACCCTGG CACCTCGGTT CTTGAGGGAG GAGCTGCAGG ATCCATGTCT 
CCCAGCAGAG TGGAAGCCAA TGTCCCCATG GTTCAGCCTG ATGTCTCACC AGCCCCCAAG 
CAGGAGGAGC CAGCCGAGAA CAGTGCGGAT ACTGGGGGCG ATGGGGAGAG CTGCCTATCT 
CAACAGGACG GAGCAGCTGG GGTGCCGGAG ACCAACGCAG CCGGTTCATC CTCAGAGGCC 
TGTGACTTCC TCAAGGAAGA CGGGAATGAT GAGGTAATGA CCCCCAGTCG ACCCAGGACC 
ACAGAAGACC TTTTTGCAGC TATTCACAGA TCCAAAAGGA AAGTCCTCGG CCGTAGAGAT 
TCAGATGATG ACCACTCCCG AAACCATTCT CCCTCCCCGC CCGTGACACC CACCGGCGCT 
GCCCCAAGCC TGGCCTCTCC AAAGCAAGTG GGGTCGATTC AGAGAAGCAT CCGAAAGAGC 
AGCACCAGCA GTGACAACTT CAAAGCTCTG CTGCTGAAAA AGGGCAGTCG TTCAGACACC 
AGCGCCCGCA TGTCTGCAGC AGAGATGCTC AAGAACACAG ACCCTAGATT CCAGAGGTCG 
AGGTCAGAGC CTTCCCCAGA TGCCCCCGAG AGCCCGTCAA GCTGCTCCCC AAGCAAGAAC 
AGAAGGGCGC AGGAGGAGTG GGCCAAGAAC GAAGGCTTGA TGCCTCGGAG TCTGTCCTTT 
TCCGGCCCCA GGTACGGCCG CAGCCGAACG CCGCCTTCTG CCGCCAGCAG CAGGTACAGC 
ATGCGGAACC GGATCCAGAG CAGCCCCATG ACCGTCATCT CGGAGGGAGA AGGGGAAGCC 
GTGGAGCCTG TGGACAGCAT AGCCCGCGGG GCTCTGGGCG CTGCGGAGGG ATGTTCCCTG 
GACGGACTGG CGAGGGAGGA GATGGACGAG GGCGGCCTGC TCTGTGGGGA GGGGCCTGCC 
GCCTCCCTGC AGCCCCAGGC CCCCGGCCCT GTGGATGGGA CAGCCAGTGC AGAGGGCAGA 
GAGCCCTCCC CACAGTGTGG CGGTTCTCTG AGCGAGGAGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005267119.1
CDS	2040..7004
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_005267062.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267062.5

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGAATGC 
GATAAGTTGC GGCATGATGG CTACCGCAGT TCTCAGTACT ACTCTCAGGG ACCCACCTTT 
GCGGCCAACG CCAGCCCATT CTGTGATGAT TACCAAGATG AAGATGAAGA AACAGATCAA 
AAGTGTTCTC TGTCTTCATC AGAAGAAGAA AGATTTATTT CCATCAGGAG ACCTAAAACA 
CCAGCCTCAA GTGACTTCTC CGACCTTAAT ACTCAGACGA ACTGGACCAA GTCGCTTCCA 
CTGCCAACAC CAGAAGAGAA GATGCGACAG CAAGCCCAAA CAGTCCAGGC TGACGTGGTG 
CCTATTAACA TAACTGGGGA GAATTTCGAT CGCCAGGCCA GCCTTCGGCG GTCTCTAATT 
TACACAGACA CTCTGGTAAG ACGACCGAAG AAAGTCAAAA GGAGAAAGAC TATTACAGGA 
GTCCCTGACA ACATACAGAA GGAGCTAGCA TCAGGCACTG GCCAAGATGA TGCTGATGGC 
CACTCAGTGT ACACCCCTGA TCACTACTCT ACACTAGGAA GGTTCAATAG CTGTCGGTCT 
GCTGGGCAGC GCTCAGAAAC CAGGGACTCC AGCTGTCAGA CGGAGGATGT GAAGGTCGTA 
CCACCTTCCA TGAGGAGAAT CAGGGCACAG AAGGGGCAAG GCATTGCTGC CCAGATGGGC 
CACTTCTCAG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ATTCTGCAGG GATCGTATTC 
CCTTCCCGCC TTGACAGTGA TGCTGGCTTC CATAGTCTTC CGCGTTCTGG AGCAAGGGCA 
AACATTCAGT CCCTTGAGCC GAGGCTGGGT GCCCTCGGCC CTGCAGGAGA CATGAATGGC 
ACTTTCCTCT ACCAGAGAGG TCACCCACAA GCAGATGAAA ACTTAGGCCA TTTAGGAGGT 
GCCTCAGGGA CTGGAACACT TTTGAGACCC AAATCCCAGG AGTTGAGACA CTTCGAGAGT 
GAAAATATAA TGAGCCCAGC GTGTGTGGTT TCTCCTCATG CAACCTACTC CACCAGCATC 
ATCCCAAATG CCACACTGTC TTCCTCTTCC GAGGTCATCG CTATTCCCAC TGCTCAGAGT 
GCGGGACAGC GGGAAAGTAA AAGTTCTGGC TCATCACATG CAAGGATAAA ATCCAGAGAC 
CACCTCATCT CCAGGCATGC TGTGAAAGGT GATCCTCAGT CTCCCGGTCG CCACTGGAAT 
GAGGGCCATG CCACCATTCT TTCACAGGAC TTAGACCCTC ATTCCCCTGG TGAACCCGCA 
CTGTTGTCCC TCTGTGACTC AGCCGTCCCT CTAAATGCTC CAGCAAATAG GGAGAATGGG 
TCCCAAGCTA TGCCGTATAA TTGTAGAAAC AACCTGGCCT TCCCAGCCCA CCCCCAAGAT 
GTGGATGGCA AGAGTGAATC TAGTTATTCA GGGGGCGGAG GGCACAGCAG CTCGGAGCCC 
TGGGAATACA AATCCTCAGG TAATGGAAGG GCATCCCCCC TGAAGCCGCA TTTAGCAACT 
CCTGGCTATT CCACTCCCAC AAGTAACATG AGCAGCTGCA GTTTGGACCA AACGTCCAAC 
AAAGAGGATG CTGGGTCGCT GTATTCTGAG GACCACGATG GCTACTGTGC ATCTGTGCAC 
ACTGACTCTG GACATGGATC TGGGAATCTG TGCAATAGCA GTGATGGCTT TGGGAACCCC 
AGGCACAGCG TGATCAATGT TTTTGTTGGA AGAGCTCAGA AAAACCAAGG GGACCGGTCC 
AATTACCAGG ATAAATCCCT ATCAAGAAAC ATCTCTTTGA AGAAAGCAAA GAAGCCTCCC 
CTGCCACCCT CCCGGACAGA CTCCCTCCGC AGGATTCCCA AGAAGAGCAG CCAGTGCAAC 
GGGCAGGTGC TCAACGAGAG CCTGATCGCC ACACTCCAGC ACTCGCTGCA GCTGAGCCTC 
CCAGGCAAGA GTGGCAGCTC GCCCTCCCAG AGCCCCTGCA GTGACTTGGA AGAGCCCTGG 
CTGCCCCGCT CCCGGAGCCA GAGCACAGTT AGTGCTGGCA GCAGCATGAC TTCCGCCACC 
ACCCCCAATG TCTACTCCCT GTGCGGGGCC ACGCCATCGC AGAGTGACAC AAGCAGCGTC 
AAGTCAGAGT ACACGGACCC CTGGGGTTAT TACATTGACT ACACGGGCAT GCAGGAAGAT 
CCGGGGAACC CGGCAGGGGG CTGTTCAACC AGCAGTGGGG TGCCCACTGG GAACGGGCCA 
GTCCGCCATG TCCAAGAAGG GTCCAGAGCC ACAATGCCCC AAGTGCCCGG TGGTTCAGTC 
AAACCAAAGA TCATGTCACC AGAGAAGTCA CACAGAGTCA TTTCTCCATC CAGTGGGTAT 
TCCAGCCAGT CGAATACACC CACAGCACTC ACCCCTGTGC CTGTGTTTTT AAAATCAGTG 
TCACCAGCAA ACGGGAAGGG GAAGCCCAAG CCCAAGGTAC CAGAAAGGAA GTCCTCTCTG 
ATATCTTCAG TATCCATTTC CTCATCGTCC ACTTCTCTTT CTTCTAGTAC TTCTACTGAA 
GGAAGTGGCA CTATGAAGAA GCTGGATCCA GCCGTGGGCT CTCCCCCGGC TCCTCCTCCT 
CCTCCTGTTC CCTCTCCTCC ATTCCCTTGT CCTGCAGACA GGTCTCCTTT CCTTCCTCCC 
CCACCTCCTG TCACAGATTG CTCCCAGGGC TCTCCTCTGC CTCACTCTCC TGTGTTCCCC 
CCTCCGCCGC CAGAAGCTCT CATTCCTTTC TGCTCCCCAC CTGATTGGTG CCTTTCTCCT 
CCCCGCCCTG CACTGAGCCC CATTCTTCCA GATTCACCTG TGTCCTTGCC ATTGCCCCCA 
CCTCTCTTAC CTTCCTCGGA ACCCCCACCT GCCCCACCTC TTGACCCCAA ATTCATGAAA 
GACACCAGGC CGCCTTTCAC AAATTCTGGC CAGCCAGAAT CCTCCCGGGG ATCCTTGAGG 
CCGCCTTCTA CCAAGGAGGA GACCAGCAGG CCCCCCATGC CCCTGATAAC CACGGAAGCA 
TTGCAGATGG TGCAGTTGAG GCCCGTGAGA AAGAACTCAG GCGCTGAGGC GGCACAGTTG 
TCTGAACGAA CAGCTCAGGA ACAACGAACT CCAGTTGCTC CACAGTACCA CTTAAAGCCA 
TCTGCTTTCC TGAAATCCCG AAATAGCACA AATGAAATGG AGAGTGAAAG CCAGCCTGCC 
TCTGTGACAA GCTCGCTTCC GACGCCTGCC AAGAGCTCGA GTCAGGGTGA CCATGGCAGT 
GCGGCTGAGC GTGGTGGCCC TGTGAGCCGC AGCCCTGGAG CTCCAAGCGC TGGGGAGGCA 
GAGGCTCGGC CCAGCCCCAG CACCACCCCA CTCCCAGACT CTTCACCCAG CAGGAAGCCA 
CCCCCCATTT CCAAGAAGCC CAAACTGTTC CTGGTGGTAC CACCTCCGCA GAAAGATTTT 
GCAGTGGAGC CCGCAGAGAA CGTGAGCGAA GCCCTCCGAG CTGTGCCCAG CCCCACGACG 
GGAGAGGAGG GCTCTGTGCA CAGCAGGGAG GCAAAAGAGA GTTCTGCAGC CCAAGCTGGC 
TCTCATGCCA CGCACCCTGG CACCTCGGTT CTTGAGGGAG GAGCTGCAGG ATCCATGTCT 
CCCAGCAGAG TGGAAGCCAA TGTCCCCATG GTTCAGCCTG ATGTCTCACC AGCCCCCAAG 
CAGGAGGAGC CAGCCGAGAA CAGTGCGGAT ACTGGGGGCG ATGGGGAGAG CTGCCTATCT 
CAACAGGACG GAGCAGCTGG GGTGCCGGAG ACCAACGCAG CCGGTTCATC CTCAGAGGCC 
TGTGACTTCC TCAAGGAAGA CGGGAATGAT GAGGTAATGA CCCCCAGTCG ACCCAGGACC 
ACAGAAGACC TTTTTGCAGC TATTCACAGA TCCAAAAGGA AAGTCCTCGG CCGTAGAGAT 
TCAGATGATG ACCACTCCCG AAACCATTCT CCCTCCCCGC CCGTGACACC CACCGGCGCT 
GCCCCAAGCC TGGCCTCTCC AAAGCAAGTG GGGTCGATTC AGAGAAGCAT CCGAAAGAGC 
AGCACCAGCA GTGACAACTT CAAAGCTCTG CTGCTGAAAA AGGGCAGTCG TTCAGACACC 
AGCGCCCGCA TGTCTGCAGC AGAGATGCTC AAGAACACAG ACCCTAGATT CCAGAGGTCG 
AGGTCAGAGC CTTCCCCAGA TGCCCCCGAG AGCCCGTCAA GCTGCTCCCC AAGCAAGAAC 
AGAAGGGCGC AGGAGGAGTG GGCCAAGAAC GAAGGCTTGA TGCCTCGGAG TCTGTCCTTT 
TCCGGCCCCA GGTACGGCCG CAGCCGAACG CCGCCTTCTG CCGCCAGCAG CAGGTACAGC 
ATGCGGAACC GGATCCAGAG CAGCCCCATG ACCGTCATCT CGGAGGGAGA AGGGGAAGCC 
GTGGAGCCTG TGGACAGCAT AGCCCGCGGG GCTCTGGGCG CTGCGGAGGG ATGTTCCCTG 
GACGGACTGG CGAGGGAGGA GATGGACGAG GGCGGCCTGC TCTGTGGGGA GGGGCCTGCC 
GCCTCCCTGC AGCCCCAGGC CCCCGGCCCT GTGGATGGGA CAGCCAGTGC AGAGGGCAGA 
GAGCCCTCCC CACAGTGTGG CGGTTCTCTG AGCGAGGAGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_017011086.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866575.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X4, mRNA.

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CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_017011086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866575.1
CDS	1859..6877
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_017011086.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011086.1

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535967.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534269.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X2, mRNA.

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CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_011535967.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535967.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534269.1
CDS	1668..6686
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535967.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535967.3

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_017011086.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866575.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X3, mRNA.

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CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_017011086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866575.1
CDS	1634..6652
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_017011086.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011086.2

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535966.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534268.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_011535966.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534268.1
CDS	1949..6967
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535966.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535966.2

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535966.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534268.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X1, mRNA.

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CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_011535966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534268.1
CDS	1724..6742
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535966.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535966.3

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535967.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534269.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X3, mRNA.

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CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_011535967.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534269.1
CDS	1893..6911
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535967.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535967.2

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535965.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534267.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X1, mRNA.

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CloneID	OHu70398
Clone ID Related Accession (Same CDS sequence)	XM_017011086.1 , XM_011535967.3 , XM_017011086.2 , XM_011535966.2 , XM_011535966.3 , XM_011535967.2 , XM_011535965.2
Accession Version	XM_011535965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534267.1
CDS	2273..7291
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535965.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535965.2

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG GGGAGAATTT CGATCGCCAG GCCAGCCTTC GGCGGTCTCT AATTTACACA 
GACACTCTGG TAAGACGACC GAAGAAAGTC AAAAGGAGAA AGACTATTAC AGGAGTCCCT 
GACAACATAC AGAAGGAGCT AGCATCAGGC ACTGGCCAAG ATGATGCTGA TGGCCACTCA 
GTGTACACCC CTGATCACTA CTCTACACTA GGAAGGTTCA ATAGCTGTCG GTCTGCTGGG 
CAGCGCTCAG AAACCAGGGA CTCCAGCTGT CAGACGGAGG ATGTGAAGGT CGTACCACCT 
TCCATGAGGA GAATCAGGGC ACAGAAGGGG CAAGGCATTG CTGCCCAGAT GGGCCACTTC 
TCAGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGATTCTG CAGGGATCGT ATTCCCTTCC 
CGCCTTGACA GTGATGCTGG CTTCCATAGT CTTCCGCGTT CTGGAGCAAG GGCAAACATT 
CAGTCCCTTG AGCCGAGGCT GGGTGCCCTC GGCCCTGCAG GAGACATGAA TGGCACTTTC 
CTCTACCAGA GAGGTCACCC ACAAGCAGAT GAAAACTTAG GCCATTTAGG AGGTGCCTCA 
GGGACTGGAA CACTTTTGAG ACCCAAATCC CAGGAGTTGA GACACTTCGA GAGTGAAAAT 
ATAATGAGCC CAGCGTGTGT GGTTTCTCCT CATGCAACCT ACTCCACCAG CATCATCCCA 
AATGCCACAC TGTCTTCCTC TTCCGAGGTC ATCGCTATTC CCACTGCTCA GAGTGCGGGA 
CAGCGGGAAA GTAAAAGTTC TGGCTCATCA CATGCAAGGA TAAAATCCAG AGACCACCTC 
ATCTCCAGGC ATGCTGTGAA AGGTGATCCT CAGTCTCCCG GTCGCCACTG GAATGAGGGC 
CATGCCACCA TTCTTTCACA GGACTTAGAC CCTCATTCCC CTGGTGAACC CGCACTGTTG 
TCCCTCTGTG ACTCAGCCGT CCCTCTAAAT GCTCCAGCAA ATAGGGAGAA TGGGTCCCAA 
GCTATGCCGT ATAATTGTAG AAACAACCTG GCCTTCCCAG CCCACCCCCA AGATGTGGAT 
GGCAAGAGTG AATCTAGTTA TTCAGGGGGC GGAGGGCACA GCAGCTCGGA GCCCTGGGAA 
TACAAATCCT CAGGTAATGG AAGGGCATCC CCCCTGAAGC CGCATTTAGC AACTCCTGGC 
TATTCCACTC CCACAAGTAA CATGAGCAGC TGCAGTTTGG ACCAAACGTC CAACAAAGAG 
GATGCTGGGT CGCTGTATTC TGAGGACCAC GATGGCTACT GTGCATCTGT GCACACTGAC 
TCTGGACATG GATCTGGGAA TCTGTGCAAT AGCAGTGATG GCTTTGGGAA CCCCAGGCAC 
AGCGTGATCA ATGTTTTTGT TGGAAGAGCT CAGAAAAACC AAGGGGACCG GTCCAATTAC 
CAGGATAAAT CCCTATCAAG AAACATCTCT TTGAAGAAAG CAAAGAAGCC TCCCCTGCCA 
CCCTCCCGGA CAGACTCCCT CCGCAGGATT CCCAAGAAGA GCAGCCAGTG CAACGGGCAG 
GTGCTCAACG AGAGCCTGAT CGCCACACTC CAGCACTCGC TGCAGCTGAG CCTCCCAGGC 
AAGAGTGGCA GCTCGCCCTC CCAGAGCCCC TGCAGTGACT TGGAAGAGCC CTGGCTGCCC 
CGCTCCCGGA GCCAGAGCAC AGTTAGTGCT GGCAGCAGCA TGACTTCCGC CACCACCCCC 
AATGTCTACT CCCTGTGCGG GGCCACGCCA TCGCAGAGTG ACACAAGCAG CGTCAAGTCA 
GAGTACACGG ACCCCTGGGG TTATTACATT GACTACACGG GCATGCAGGA AGATCCGGGG 
AACCCGGCAG GGGGCTGTTC AACCAGCAGT GGGGTGCCCA CTGGGAACGG GCCAGTCCGC 
CATGTCCAAG AAGGGTCCAG AGCCACAATG CCCCAAGTGC CCGGTGGTTC AGTCAAACCA 
AAGATCATGT CACCAGAGAA GTCACACAGA GTCATTTCTC CATCCAGTGG GTATTCCAGC 
CAGTCGAATA CACCCACAGC ACTCACCCCT GTGCCTGTGT TTTTAAAATC AGTGTCACCA 
GCAAACGGGA AGGGGAAGCC CAAGCCCAAG GTACCAGAAA GGAAGTCCTC TCTGATATCT 
TCAGTATCCA TTTCCTCATC GTCCACTTCT CTTTCTTCTA GTACTTCTAC TGAAGGAAGT 
GGCACTATGA AGAAGCTGGA TCCAGCCGTG GGCTCTCCCC CGGCTCCTCC TCCTCCTCCT 
GTTCCCTCTC CTCCATTCCC TTGTCCTGCA GACAGGTCTC CTTTCCTTCC TCCCCCACCT 
CCTGTCACAG ATTGCTCCCA GGGCTCTCCT CTGCCTCACT CTCCTGTGTT CCCCCCTCCG 
CCGCCAGAAG CTCTCATTCC TTTCTGCTCC CCACCTGATT GGTGCCTTTC TCCTCCCCGC 
CCTGCACTGA GCCCCATTCT TCCAGATTCA CCTGTGTCCT TGCCATTGCC CCCACCTCTC 
TTACCTTCCT CGGAACCCCC ACCTGCCCCA CCTCTTGACC CCAAATTCAT GAAAGACACC 
AGGCCGCCTT TCACAAATTC TGGCCAGCCA GAATCCTCCC GGGGATCCTT GAGGCCGCCT 
TCTACCAAGG AGGAGACCAG CAGGCCCCCC ATGCCCCTGA TAACCACGGA AGCATTGCAG 
ATGGTGCAGT TGAGGCCCGT GAGAAAGAAC TCAGGCGCTG AGGCGGCACA GTTGTCTGAA 
CGAACAGCTC AGGAACAACG AACTCCAGTT GCTCCACAGT ACCACTTAAA GCCATCTGCT 
TTCCTGAAAT CCCGAAATAG CACAAATGAA ATGGAGAGTG AAAGCCAGCC TGCCTCTGTG 
ACAAGCTCGC TTCCGACGCC TGCCAAGAGC TCGAGTCAGG GTGACCATGG CAGTGCGGCT 
GAGCGTGGTG GCCCTGTGAG CCGCAGCCCT GGAGCTCCAA GCGCTGGGGA GGCAGAGGCT 
CGGCCCAGCC CCAGCACCAC CCCACTCCCA GACTCTTCAC CCAGCAGGAA GCCACCCCCC 
ATTTCCAAGA AGCCCAAACT GTTCCTGGTG GTACCACCTC CGCAGAAAGA TTTTGCAGTG 
GAGCCCGCAG AGAACGTGAG CGAAGCCCTC CGAGCTGTGC CCAGCCCCAC GACGGGAGAG 
GAGGGCTCTG TGCACAGCAG GGAGGCAAAA GAGAGTTCTG CAGCCCAAGC TGGCTCTCAT 
GCCACGCACC CTGGCACCTC GGTTCTTGAG GGAGGAGCTG CAGGATCCAT GTCTCCCAGC 
AGAGTGGAAG CCAATGTCCC CATGGTTCAG CCTGATGTCT CACCAGCCCC CAAGCAGGAG 
GAGCCAGCCG AGAACAGTGC GGATACTGGG GGCGATGGGG AGAGCTGCCT ATCTCAACAG 
GACGGAGCAG CTGGGGTGCC GGAGACCAAC GCAGCCGGTT CATCCTCAGA GGCCTGTGAC 
TTCCTCAAGG AAGACGGGAA TGATGAGGTA ATGACCCCCA GTCGACCCAG GACCACAGAA 
GACCTTTTTG CAGCTATTCA CAGATCCAAA AGGAAAGTCC TCGGCCGTAG AGATTCAGAT 
GATGACCACT CCCGAAACCA TTCTCCCTCC CCGCCCGTGA CACCCACCGG CGCTGCCCCA 
AGCCTGGCCT CTCCAAAGCA AGTGGGGTCG ATTCAGAGAA GCATCCGAAA GAGCAGCACC 
AGCAGTGACA ACTTCAAAGC TCTGCTGCTG AAAAAGGGCA GTCGTTCAGA CACCAGCGCC 
CGCATGTCTG CAGCAGAGAT GCTCAAGAAC ACAGACCCTA GATTCCAGAG GTCGAGGTCA 
GAGCCTTCCC CAGATGCCCC CGAGAGCCCG TCAAGCTGCT CCCCAAGCAA GAACAGAAGG 
GCGCAGGAGG AGTGGGCCAA GAACGAAGGC TTGATGCCTC GGAGTCTGTC CTTTTCCGGC 
CCCAGGTACG GCCGCAGCCG AACGCCGCCT TCTGCCGCCA GCAGCAGGTA CAGCATGCGG 
AACCGGATCC AGAGCAGCCC CATGACCGTC ATCTCGGAGG GAGAAGGGGA AGCCGTGGAG 
CCTGTGGACA GCATAGCCCG CGGGGCTCTG GGCGCTGCGG AGGGATGTTC CCTGGACGGA 
CTGGCGAGGG AGGAGATGGA CGAGGGCGGC CTGCTCTGTG GGGAGGGGCC TGCCGCCTCC 
CTGCAGCCCC AGGCCCCCGG CCCTGTGGAT GGGACAGCCA GTGCAGAGGG CAGAGAGCCC 
TCCCCACAGT GTGGCGGTTC TCTGAGCGAG GAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535968.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534270.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X6, mRNA.

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CloneID	OHu70399
Clone ID Related Accession (Same CDS sequence)	XM_011535968.3 , XM_011535968.2
Accession Version	XM_011535968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG CATCAGGCAC TGGCCAAGAT GATGCTGATG GCCACTCAGT GTACACCCCT 
GATCACTACT CTACACTAGG AAGGTTCAAT AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA 
ACCAGGGACT CCAGCTGTCA GACGGAGGAT GTGAAGGTCG TACCACCTTC CATGAGGAGA 
ATCAGGGCAC AGAAGGGGCA AGGCATTGCT GCCCAGATGG GCCACTTCTC AGGTTCCTCT 
GGCAACATGT CTGTGCTGAG CGATTCTGCA GGGATCGTAT TCCCTTCCCG CCTTGACAGT 
GATGCTGGCT TCCATAGTCT TCCGCGTTCT GGAGCAAGGG CAAACATTCA GTCCCTTGAG 
CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA GACATGAATG GCACTTTCCT CTACCAGAGA 
GGTCACCCAC AAGCAGATGA AAACTTAGGC CATTTAGGAG GTGCCTCAGG GACTGGAACA 
CTTTTGAGAC CCAAATCCCA GGAGTTGAGA CACTTCGAGA GTGAAAATAT AATGAGCCCA 
GCGTGTGTGG TTTCTCCTCA TGCAACCTAC TCCACCAGCA TCATCCCAAA TGCCACACTG 
TCTTCCTCTT CCGAGGTCAT CGCTATTCCC ACTGCTCAGA GTGCGGGACA GCGGGAAAGT 
AAAAGTTCTG GCTCATCACA TGCAAGGATA AAATCCAGAG ACCACCTCAT CTCCAGGCAT 
GCTGTGAAAG GTGATCCTCA GTCTCCCGGT CGCCACTGGA ATGAGGGCCA TGCCACCATT 
CTTTCACAGG ACTTAGACCC TCATTCCCCT GGTGAACCCG CACTGTTGTC CCTCTGTGAC 
TCAGCCGTCC CTCTAAATGC TCCAGCAAAT AGGGAGAATG GGTCCCAAGC TATGCCGTAT 
AATTGTAGAA ACAACCTGGC CTTCCCAGCC CACCCCCAAG ATGTGGATGG CAAGAGTGAA 
TCTAGTTATT CAGGGGGCGG AGGGCACAGC AGCTCGGAGC CCTGGGAATA CAAATCCTCA 
GGTAATGGAA GGGCATCCCC CCTGAAGCCG CATTTAGCAA CTCCTGGCTA TTCCACTCCC 
ACAAGTAACA TGAGCAGCTG CAGTTTGGAC CAAACGTCCA ACAAAGAGGA TGCTGGGTCG 
CTGTATTCTG AGGACCACGA TGGCTACTGT GCATCTGTGC ACACTGACTC TGGACATGGA 
TCTGGGAATC TGTGCAATAG CAGTGATGGC TTTGGGAACC CCAGGCACAG CGTGATCAAT 
GTTTTTGTTG GAAGAGCTCA GAAAAACCAA GGGGACCGGT CCAATTACCA GGATAAATCC 
CTATCAAGAA ACATCTCTTT GAAGAAAGCA AAGAAGCCTC CCCTGCCACC CTCCCGGACA 
GACTCCCTCC GCAGGATTCC CAAGAAGAGC AGCCAGTGCA ACGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCACACTCCA GCACTCGCTG CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC 
TCGCCCTCCC AGAGCCCCTG CAGTGACTTG GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC 
CAGAGCACAG TTAGTGCTGG CAGCAGCATG ACTTCCGCCA CCACCCCCAA TGTCTACTCC 
CTGTGCGGGG CCACGCCATC GCAGAGTGAC ACAAGCAGCG TCAAGTCAGA GTACACGGAC 
CCCTGGGGTT ATTACATTGA CTACACGGGC ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG 
GGCTGTTCAA CCAGCAGTGG GGTGCCCACT GGGAACGGGC CAGTCCGCCA TGTCCAAGAA 
GGGTCCAGAG CCACAATGCC CCAAGTGCCC GGTGGTTCAG TCAAACCAAA GATCATGTCA 
CCAGAGAAGT CACACAGAGT CATTTCTCCA TCCAGTGGGT ATTCCAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCTGT GCCTGTGTTT TTAAAATCAG TGTCACCAGC AAACGGGAAG 
GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG AAGTCCTCTC TGATATCTTC AGTATCCATT 
TCCTCATCGT CCACTTCTCT TTCTTCTAGT ACTTCTACTG AAGGAAGTGG CACTATGAAG 
AAGCTGGATC CAGCCGTGGG CTCTCCCCCG GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT 
CCATTCCCTT GTCCTGCAGA CAGGTCTCCT TTCCTTCCTC CCCCACCTCC TGTCACAGAT 
TGCTCCCAGG GCTCTCCTCT GCCTCACTCT CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT 
CTCATTCCTT TCTGCTCCCC ACCTGATTGG TGCCTTTCTC CTCCCCGCCC TGCACTGAGC 
CCCATTCTTC CAGATTCACC TGTGTCCTTG CCATTGCCCC CACCTCTCTT ACCTTCCTCG 
GAACCCCCAC CTGCCCCACC TCTTGACCCC AAATTCATGA AAGACACCAG GCCGCCTTTC 
ACAAATTCTG GCCAGCCAGA ATCCTCCCGG GGATCCTTGA GGCCGCCTTC TACCAAGGAG 
GAGACCAGCA GGCCCCCCAT GCCCCTGATA ACCACGGAAG CATTGCAGAT GGTGCAGTTG 
AGGCCCGTGA GAAAGAACTC AGGCGCTGAG GCGGCACAGT TGTCTGAACG AACAGCTCAG 
GAACAACGAA CTCCAGTTGC TCCACAGTAC CACTTAAAGC CATCTGCTTT CCTGAAATCC 
CGAAATAGCA CAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT 
CCGACGCCTG CCAAGAGCTC GAGTCAGGGT GACCATGGCA GTGCGGCTGA GCGTGGTGGC 
CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC 
AGCACCACCC CACTCCCAGA CTCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG 
CCCAAACTGT TCCTGGTGGT ACCACCTCCG CAGAAAGATT TTGCAGTGGA GCCCGCAGAG 
AACGTGAGCG AAGCCCTCCG AGCTGTGCCC AGCCCCACGA CGGGAGAGGA GGGCTCTGTG 
CACAGCAGGG AGGCAAAAGA GAGTTCTGCA GCCCAAGCTG GCTCTCATGC CACGCACCCT 
GGCACCTCGG TTCTTGAGGG AGGAGCTGCA GGATCCATGT CTCCCAGCAG AGTGGAAGCC 
AATGTCCCCA TGGTTCAGCC TGATGTCTCA CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG 
AACAGTGCGG ATACTGGGGG CGATGGGGAG AGCTGCCTAT CTCAACAGGA CGGAGCAGCT 
GGGGTGCCGG AGACCAACGC AGCCGGTTCA TCCTCAGAGG CCTGTGACTT CCTCAAGGAA 
GACGGGAATG ATGAGGTAAT GACCCCCAGT CGACCCAGGA CCACAGAAGA CCTTTTTGCA 
GCTATTCACA GATCCAAAAG GAAAGTCCTC GGCCGTAGAG ATTCAGATGA TGACCACTCC 
CGAAACCATT CTCCCTCCCC GCCCGTGACA CCCACCGGCG CTGCCCCAAG CCTGGCCTCT 
CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC ATCCGAAAGA GCAGCACCAG CAGTGACAAC 
TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT CGTTCAGACA CCAGCGCCCG CATGTCTGCA 
GCAGAGATGC TCAAGAACAC AGACCCTAGA TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA 
GATGCCCCCG AGAGCCCGTC AAGCTGCTCC CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG 
TGGGCCAAGA ACGAAGGCTT GATGCCTCGG AGTCTGTCCT TTTCCGGCCC CAGGTACGGC 
CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC AGCAGGTACA GCATGCGGAA CCGGATCCAG 
AGCAGCCCCA TGACCGTCAT CTCGGAGGGA GAAGGGGAAG CCGTGGAGCC TGTGGACAGC 
ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG GGATGTTCCC TGGACGGACT GGCGAGGGAG 
GAGATGGACG AGGGCGGCCT GCTCTGTGGG GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG 
GCCCCCGGCC CTGTGGATGG GACAGCCAGT GCAGAGGGCA GAGAGCCCTC CCCACAGTGT 
GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534270.1
CDS	2047..6933
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535968.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535968.3

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG CATCAGGCAC TGGCCAAGAT GATGCTGATG GCCACTCAGT GTACACCCCT 
GATCACTACT CTACACTAGG AAGGTTCAAT AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA 
ACCAGGGACT CCAGCTGTCA GACGGAGGAT GTGAAGGTCG TACCACCTTC CATGAGGAGA 
ATCAGGGCAC AGAAGGGGCA AGGCATTGCT GCCCAGATGG GCCACTTCTC AGGTTCCTCT 
GGCAACATGT CTGTGCTGAG CGATTCTGCA GGGATCGTAT TCCCTTCCCG CCTTGACAGT 
GATGCTGGCT TCCATAGTCT TCCGCGTTCT GGAGCAAGGG CAAACATTCA GTCCCTTGAG 
CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA GACATGAATG GCACTTTCCT CTACCAGAGA 
GGTCACCCAC AAGCAGATGA AAACTTAGGC CATTTAGGAG GTGCCTCAGG GACTGGAACA 
CTTTTGAGAC CCAAATCCCA GGAGTTGAGA CACTTCGAGA GTGAAAATAT AATGAGCCCA 
GCGTGTGTGG TTTCTCCTCA TGCAACCTAC TCCACCAGCA TCATCCCAAA TGCCACACTG 
TCTTCCTCTT CCGAGGTCAT CGCTATTCCC ACTGCTCAGA GTGCGGGACA GCGGGAAAGT 
AAAAGTTCTG GCTCATCACA TGCAAGGATA AAATCCAGAG ACCACCTCAT CTCCAGGCAT 
GCTGTGAAAG GTGATCCTCA GTCTCCCGGT CGCCACTGGA ATGAGGGCCA TGCCACCATT 
CTTTCACAGG ACTTAGACCC TCATTCCCCT GGTGAACCCG CACTGTTGTC CCTCTGTGAC 
TCAGCCGTCC CTCTAAATGC TCCAGCAAAT AGGGAGAATG GGTCCCAAGC TATGCCGTAT 
AATTGTAGAA ACAACCTGGC CTTCCCAGCC CACCCCCAAG ATGTGGATGG CAAGAGTGAA 
TCTAGTTATT CAGGGGGCGG AGGGCACAGC AGCTCGGAGC CCTGGGAATA CAAATCCTCA 
GGTAATGGAA GGGCATCCCC CCTGAAGCCG CATTTAGCAA CTCCTGGCTA TTCCACTCCC 
ACAAGTAACA TGAGCAGCTG CAGTTTGGAC CAAACGTCCA ACAAAGAGGA TGCTGGGTCG 
CTGTATTCTG AGGACCACGA TGGCTACTGT GCATCTGTGC ACACTGACTC TGGACATGGA 
TCTGGGAATC TGTGCAATAG CAGTGATGGC TTTGGGAACC CCAGGCACAG CGTGATCAAT 
GTTTTTGTTG GAAGAGCTCA GAAAAACCAA GGGGACCGGT CCAATTACCA GGATAAATCC 
CTATCAAGAA ACATCTCTTT GAAGAAAGCA AAGAAGCCTC CCCTGCCACC CTCCCGGACA 
GACTCCCTCC GCAGGATTCC CAAGAAGAGC AGCCAGTGCA ACGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCACACTCCA GCACTCGCTG CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC 
TCGCCCTCCC AGAGCCCCTG CAGTGACTTG GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC 
CAGAGCACAG TTAGTGCTGG CAGCAGCATG ACTTCCGCCA CCACCCCCAA TGTCTACTCC 
CTGTGCGGGG CCACGCCATC GCAGAGTGAC ACAAGCAGCG TCAAGTCAGA GTACACGGAC 
CCCTGGGGTT ATTACATTGA CTACACGGGC ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG 
GGCTGTTCAA CCAGCAGTGG GGTGCCCACT GGGAACGGGC CAGTCCGCCA TGTCCAAGAA 
GGGTCCAGAG CCACAATGCC CCAAGTGCCC GGTGGTTCAG TCAAACCAAA GATCATGTCA 
CCAGAGAAGT CACACAGAGT CATTTCTCCA TCCAGTGGGT ATTCCAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCTGT GCCTGTGTTT TTAAAATCAG TGTCACCAGC AAACGGGAAG 
GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG AAGTCCTCTC TGATATCTTC AGTATCCATT 
TCCTCATCGT CCACTTCTCT TTCTTCTAGT ACTTCTACTG AAGGAAGTGG CACTATGAAG 
AAGCTGGATC CAGCCGTGGG CTCTCCCCCG GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT 
CCATTCCCTT GTCCTGCAGA CAGGTCTCCT TTCCTTCCTC CCCCACCTCC TGTCACAGAT 
TGCTCCCAGG GCTCTCCTCT GCCTCACTCT CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT 
CTCATTCCTT TCTGCTCCCC ACCTGATTGG TGCCTTTCTC CTCCCCGCCC TGCACTGAGC 
CCCATTCTTC CAGATTCACC TGTGTCCTTG CCATTGCCCC CACCTCTCTT ACCTTCCTCG 
GAACCCCCAC CTGCCCCACC TCTTGACCCC AAATTCATGA AAGACACCAG GCCGCCTTTC 
ACAAATTCTG GCCAGCCAGA ATCCTCCCGG GGATCCTTGA GGCCGCCTTC TACCAAGGAG 
GAGACCAGCA GGCCCCCCAT GCCCCTGATA ACCACGGAAG CATTGCAGAT GGTGCAGTTG 
AGGCCCGTGA GAAAGAACTC AGGCGCTGAG GCGGCACAGT TGTCTGAACG AACAGCTCAG 
GAACAACGAA CTCCAGTTGC TCCACAGTAC CACTTAAAGC CATCTGCTTT CCTGAAATCC 
CGAAATAGCA CAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT 
CCGACGCCTG CCAAGAGCTC GAGTCAGGGT GACCATGGCA GTGCGGCTGA GCGTGGTGGC 
CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC 
AGCACCACCC CACTCCCAGA CTCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG 
CCCAAACTGT TCCTGGTGGT ACCACCTCCG CAGAAAGATT TTGCAGTGGA GCCCGCAGAG 
AACGTGAGCG AAGCCCTCCG AGCTGTGCCC AGCCCCACGA CGGGAGAGGA GGGCTCTGTG 
CACAGCAGGG AGGCAAAAGA GAGTTCTGCA GCCCAAGCTG GCTCTCATGC CACGCACCCT 
GGCACCTCGG TTCTTGAGGG AGGAGCTGCA GGATCCATGT CTCCCAGCAG AGTGGAAGCC 
AATGTCCCCA TGGTTCAGCC TGATGTCTCA CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG 
AACAGTGCGG ATACTGGGGG CGATGGGGAG AGCTGCCTAT CTCAACAGGA CGGAGCAGCT 
GGGGTGCCGG AGACCAACGC AGCCGGTTCA TCCTCAGAGG CCTGTGACTT CCTCAAGGAA 
GACGGGAATG ATGAGGTAAT GACCCCCAGT CGACCCAGGA CCACAGAAGA CCTTTTTGCA 
GCTATTCACA GATCCAAAAG GAAAGTCCTC GGCCGTAGAG ATTCAGATGA TGACCACTCC 
CGAAACCATT CTCCCTCCCC GCCCGTGACA CCCACCGGCG CTGCCCCAAG CCTGGCCTCT 
CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC ATCCGAAAGA GCAGCACCAG CAGTGACAAC 
TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT CGTTCAGACA CCAGCGCCCG CATGTCTGCA 
GCAGAGATGC TCAAGAACAC AGACCCTAGA TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA 
GATGCCCCCG AGAGCCCGTC AAGCTGCTCC CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG 
TGGGCCAAGA ACGAAGGCTT GATGCCTCGG AGTCTGTCCT TTTCCGGCCC CAGGTACGGC 
CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC AGCAGGTACA GCATGCGGAA CCGGATCCAG 
AGCAGCCCCA TGACCGTCAT CTCGGAGGGA GAAGGGGAAG CCGTGGAGCC TGTGGACAGC 
ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG GGATGTTCCC TGGACGGACT GGCGAGGGAG 
GAGATGGACG AGGGCGGCCT GCTCTGTGGG GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG 
GCCCCCGGCC CTGTGGATGG GACAGCCAGT GCAGAGGGCA GAGAGCCCTC CCCACAGTGT 
GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535968.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534270.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X7, mRNA.

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CloneID	OHu70399
Clone ID Related Accession (Same CDS sequence)	XM_011535968.3 , XM_011535968.2
Accession Version	XM_011535968.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG CATCAGGCAC TGGCCAAGAT GATGCTGATG GCCACTCAGT GTACACCCCT 
GATCACTACT CTACACTAGG AAGGTTCAAT AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA 
ACCAGGGACT CCAGCTGTCA GACGGAGGAT GTGAAGGTCG TACCACCTTC CATGAGGAGA 
ATCAGGGCAC AGAAGGGGCA AGGCATTGCT GCCCAGATGG GCCACTTCTC AGGTTCCTCT 
GGCAACATGT CTGTGCTGAG CGATTCTGCA GGGATCGTAT TCCCTTCCCG CCTTGACAGT 
GATGCTGGCT TCCATAGTCT TCCGCGTTCT GGAGCAAGGG CAAACATTCA GTCCCTTGAG 
CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA GACATGAATG GCACTTTCCT CTACCAGAGA 
GGTCACCCAC AAGCAGATGA AAACTTAGGC CATTTAGGAG GTGCCTCAGG GACTGGAACA 
CTTTTGAGAC CCAAATCCCA GGAGTTGAGA CACTTCGAGA GTGAAAATAT AATGAGCCCA 
GCGTGTGTGG TTTCTCCTCA TGCAACCTAC TCCACCAGCA TCATCCCAAA TGCCACACTG 
TCTTCCTCTT CCGAGGTCAT CGCTATTCCC ACTGCTCAGA GTGCGGGACA GCGGGAAAGT 
AAAAGTTCTG GCTCATCACA TGCAAGGATA AAATCCAGAG ACCACCTCAT CTCCAGGCAT 
GCTGTGAAAG GTGATCCTCA GTCTCCCGGT CGCCACTGGA ATGAGGGCCA TGCCACCATT 
CTTTCACAGG ACTTAGACCC TCATTCCCCT GGTGAACCCG CACTGTTGTC CCTCTGTGAC 
TCAGCCGTCC CTCTAAATGC TCCAGCAAAT AGGGAGAATG GGTCCCAAGC TATGCCGTAT 
AATTGTAGAA ACAACCTGGC CTTCCCAGCC CACCCCCAAG ATGTGGATGG CAAGAGTGAA 
TCTAGTTATT CAGGGGGCGG AGGGCACAGC AGCTCGGAGC CCTGGGAATA CAAATCCTCA 
GGTAATGGAA GGGCATCCCC CCTGAAGCCG CATTTAGCAA CTCCTGGCTA TTCCACTCCC 
ACAAGTAACA TGAGCAGCTG CAGTTTGGAC CAAACGTCCA ACAAAGAGGA TGCTGGGTCG 
CTGTATTCTG AGGACCACGA TGGCTACTGT GCATCTGTGC ACACTGACTC TGGACATGGA 
TCTGGGAATC TGTGCAATAG CAGTGATGGC TTTGGGAACC CCAGGCACAG CGTGATCAAT 
GTTTTTGTTG GAAGAGCTCA GAAAAACCAA GGGGACCGGT CCAATTACCA GGATAAATCC 
CTATCAAGAA ACATCTCTTT GAAGAAAGCA AAGAAGCCTC CCCTGCCACC CTCCCGGACA 
GACTCCCTCC GCAGGATTCC CAAGAAGAGC AGCCAGTGCA ACGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCACACTCCA GCACTCGCTG CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC 
TCGCCCTCCC AGAGCCCCTG CAGTGACTTG GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC 
CAGAGCACAG TTAGTGCTGG CAGCAGCATG ACTTCCGCCA CCACCCCCAA TGTCTACTCC 
CTGTGCGGGG CCACGCCATC GCAGAGTGAC ACAAGCAGCG TCAAGTCAGA GTACACGGAC 
CCCTGGGGTT ATTACATTGA CTACACGGGC ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG 
GGCTGTTCAA CCAGCAGTGG GGTGCCCACT GGGAACGGGC CAGTCCGCCA TGTCCAAGAA 
GGGTCCAGAG CCACAATGCC CCAAGTGCCC GGTGGTTCAG TCAAACCAAA GATCATGTCA 
CCAGAGAAGT CACACAGAGT CATTTCTCCA TCCAGTGGGT ATTCCAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCTGT GCCTGTGTTT TTAAAATCAG TGTCACCAGC AAACGGGAAG 
GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG AAGTCCTCTC TGATATCTTC AGTATCCATT 
TCCTCATCGT CCACTTCTCT TTCTTCTAGT ACTTCTACTG AAGGAAGTGG CACTATGAAG 
AAGCTGGATC CAGCCGTGGG CTCTCCCCCG GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT 
CCATTCCCTT GTCCTGCAGA CAGGTCTCCT TTCCTTCCTC CCCCACCTCC TGTCACAGAT 
TGCTCCCAGG GCTCTCCTCT GCCTCACTCT CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT 
CTCATTCCTT TCTGCTCCCC ACCTGATTGG TGCCTTTCTC CTCCCCGCCC TGCACTGAGC 
CCCATTCTTC CAGATTCACC TGTGTCCTTG CCATTGCCCC CACCTCTCTT ACCTTCCTCG 
GAACCCCCAC CTGCCCCACC TCTTGACCCC AAATTCATGA AAGACACCAG GCCGCCTTTC 
ACAAATTCTG GCCAGCCAGA ATCCTCCCGG GGATCCTTGA GGCCGCCTTC TACCAAGGAG 
GAGACCAGCA GGCCCCCCAT GCCCCTGATA ACCACGGAAG CATTGCAGAT GGTGCAGTTG 
AGGCCCGTGA GAAAGAACTC AGGCGCTGAG GCGGCACAGT TGTCTGAACG AACAGCTCAG 
GAACAACGAA CTCCAGTTGC TCCACAGTAC CACTTAAAGC CATCTGCTTT CCTGAAATCC 
CGAAATAGCA CAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT 
CCGACGCCTG CCAAGAGCTC GAGTCAGGGT GACCATGGCA GTGCGGCTGA GCGTGGTGGC 
CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC 
AGCACCACCC CACTCCCAGA CTCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG 
CCCAAACTGT TCCTGGTGGT ACCACCTCCG CAGAAAGATT TTGCAGTGGA GCCCGCAGAG 
AACGTGAGCG AAGCCCTCCG AGCTGTGCCC AGCCCCACGA CGGGAGAGGA GGGCTCTGTG 
CACAGCAGGG AGGCAAAAGA GAGTTCTGCA GCCCAAGCTG GCTCTCATGC CACGCACCCT 
GGCACCTCGG TTCTTGAGGG AGGAGCTGCA GGATCCATGT CTCCCAGCAG AGTGGAAGCC 
AATGTCCCCA TGGTTCAGCC TGATGTCTCA CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG 
AACAGTGCGG ATACTGGGGG CGATGGGGAG AGCTGCCTAT CTCAACAGGA CGGAGCAGCT 
GGGGTGCCGG AGACCAACGC AGCCGGTTCA TCCTCAGAGG CCTGTGACTT CCTCAAGGAA 
GACGGGAATG ATGAGGTAAT GACCCCCAGT CGACCCAGGA CCACAGAAGA CCTTTTTGCA 
GCTATTCACA GATCCAAAAG GAAAGTCCTC GGCCGTAGAG ATTCAGATGA TGACCACTCC 
CGAAACCATT CTCCCTCCCC GCCCGTGACA CCCACCGGCG CTGCCCCAAG CCTGGCCTCT 
CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC ATCCGAAAGA GCAGCACCAG CAGTGACAAC 
TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT CGTTCAGACA CCAGCGCCCG CATGTCTGCA 
GCAGAGATGC TCAAGAACAC AGACCCTAGA TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA 
GATGCCCCCG AGAGCCCGTC AAGCTGCTCC CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG 
TGGGCCAAGA ACGAAGGCTT GATGCCTCGG AGTCTGTCCT TTTCCGGCCC CAGGTACGGC 
CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC AGCAGGTACA GCATGCGGAA CCGGATCCAG 
AGCAGCCCCA TGACCGTCAT CTCGGAGGGA GAAGGGGAAG CCGTGGAGCC TGTGGACAGC 
ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG GGATGTTCCC TGGACGGACT GGCGAGGGAG 
GAGATGGACG AGGGCGGCCT GCTCTGTGGG GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG 
GCCCCCGGCC CTGTGGATGG GACAGCCAGT GCAGAGGGCA GAGAGCCCTC CCCACAGTGT 
GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534270.1
CDS	2273..7159
Translation	MKKEGSSGSFRLQPNTGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEVDDENWIYRAQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535968.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535968.2

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTTC AGGTTCCTTC AGACTCCAGC CGAATACAGG TTCTCTCTCA 
CGTGCTGTAA GTTGGATAAA TTTCTCCTCC TTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGTGATGGAG AGCTCTCGGT TTGTGGGCAG CAGGTGGAAG TGGATGACGA AAACTGGATA 
TACAGAGCCC AGCCCAGAAA AGCGGTTTCC AACCTAGATG AGGAAAGCCG ATGGACAGTC 
CACTACACTG CCCCGTGGCA CCAGCAGGAG AATGTGTTCC TTCCCACCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCCAAG CTCAACCTCA AATCAGTACT GAGGGTGATT 
CCAGAAGATG AGATGGGAGA CCAGAATTCT GGTTGCAGTG TTGTCAGTGA ATGCGATAAG 
TTGCGGCATG ATGGCTACCG CAGTTCTCAG TACTACTCTC AGGGACCCAC CTTTGCGGCC 
AACGCCAGCC CATTCTGTGA TGATTACCAA GATGAAGATG AAGAAACAGA TCAAAAGTGT 
TCTCTGTCTT CATCAGAAGA AGAAAGATTT ATTTCCATCA GGAGACCTAA AACACCAGCC 
TCAAGTGACT TCTCCGACCT TAATACTCAG ACGAACTGGA CCAAGTCGCT TCCACTGCCA 
ACACCAGAAG AGAAGATGCG ACAGCAAGCC CAAACAGTCC AGGCTGACGT GGTGCCTATT 
AACATAACTG CATCAGGCAC TGGCCAAGAT GATGCTGATG GCCACTCAGT GTACACCCCT 
GATCACTACT CTACACTAGG AAGGTTCAAT AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA 
ACCAGGGACT CCAGCTGTCA GACGGAGGAT GTGAAGGTCG TACCACCTTC CATGAGGAGA 
ATCAGGGCAC AGAAGGGGCA AGGCATTGCT GCCCAGATGG GCCACTTCTC AGGTTCCTCT 
GGCAACATGT CTGTGCTGAG CGATTCTGCA GGGATCGTAT TCCCTTCCCG CCTTGACAGT 
GATGCTGGCT TCCATAGTCT TCCGCGTTCT GGAGCAAGGG CAAACATTCA GTCCCTTGAG 
CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA GACATGAATG GCACTTTCCT CTACCAGAGA 
GGTCACCCAC AAGCAGATGA AAACTTAGGC CATTTAGGAG GTGCCTCAGG GACTGGAACA 
CTTTTGAGAC CCAAATCCCA GGAGTTGAGA CACTTCGAGA GTGAAAATAT AATGAGCCCA 
GCGTGTGTGG TTTCTCCTCA TGCAACCTAC TCCACCAGCA TCATCCCAAA TGCCACACTG 
TCTTCCTCTT CCGAGGTCAT CGCTATTCCC ACTGCTCAGA GTGCGGGACA GCGGGAAAGT 
AAAAGTTCTG GCTCATCACA TGCAAGGATA AAATCCAGAG ACCACCTCAT CTCCAGGCAT 
GCTGTGAAAG GTGATCCTCA GTCTCCCGGT CGCCACTGGA ATGAGGGCCA TGCCACCATT 
CTTTCACAGG ACTTAGACCC TCATTCCCCT GGTGAACCCG CACTGTTGTC CCTCTGTGAC 
TCAGCCGTCC CTCTAAATGC TCCAGCAAAT AGGGAGAATG GGTCCCAAGC TATGCCGTAT 
AATTGTAGAA ACAACCTGGC CTTCCCAGCC CACCCCCAAG ATGTGGATGG CAAGAGTGAA 
TCTAGTTATT CAGGGGGCGG AGGGCACAGC AGCTCGGAGC CCTGGGAATA CAAATCCTCA 
GGTAATGGAA GGGCATCCCC CCTGAAGCCG CATTTAGCAA CTCCTGGCTA TTCCACTCCC 
ACAAGTAACA TGAGCAGCTG CAGTTTGGAC CAAACGTCCA ACAAAGAGGA TGCTGGGTCG 
CTGTATTCTG AGGACCACGA TGGCTACTGT GCATCTGTGC ACACTGACTC TGGACATGGA 
TCTGGGAATC TGTGCAATAG CAGTGATGGC TTTGGGAACC CCAGGCACAG CGTGATCAAT 
GTTTTTGTTG GAAGAGCTCA GAAAAACCAA GGGGACCGGT CCAATTACCA GGATAAATCC 
CTATCAAGAA ACATCTCTTT GAAGAAAGCA AAGAAGCCTC CCCTGCCACC CTCCCGGACA 
GACTCCCTCC GCAGGATTCC CAAGAAGAGC AGCCAGTGCA ACGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCACACTCCA GCACTCGCTG CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC 
TCGCCCTCCC AGAGCCCCTG CAGTGACTTG GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC 
CAGAGCACAG TTAGTGCTGG CAGCAGCATG ACTTCCGCCA CCACCCCCAA TGTCTACTCC 
CTGTGCGGGG CCACGCCATC GCAGAGTGAC ACAAGCAGCG TCAAGTCAGA GTACACGGAC 
CCCTGGGGTT ATTACATTGA CTACACGGGC ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG 
GGCTGTTCAA CCAGCAGTGG GGTGCCCACT GGGAACGGGC CAGTCCGCCA TGTCCAAGAA 
GGGTCCAGAG CCACAATGCC CCAAGTGCCC GGTGGTTCAG TCAAACCAAA GATCATGTCA 
CCAGAGAAGT CACACAGAGT CATTTCTCCA TCCAGTGGGT ATTCCAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCTGT GCCTGTGTTT TTAAAATCAG TGTCACCAGC AAACGGGAAG 
GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG AAGTCCTCTC TGATATCTTC AGTATCCATT 
TCCTCATCGT CCACTTCTCT TTCTTCTAGT ACTTCTACTG AAGGAAGTGG CACTATGAAG 
AAGCTGGATC CAGCCGTGGG CTCTCCCCCG GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT 
CCATTCCCTT GTCCTGCAGA CAGGTCTCCT TTCCTTCCTC CCCCACCTCC TGTCACAGAT 
TGCTCCCAGG GCTCTCCTCT GCCTCACTCT CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT 
CTCATTCCTT TCTGCTCCCC ACCTGATTGG TGCCTTTCTC CTCCCCGCCC TGCACTGAGC 
CCCATTCTTC CAGATTCACC TGTGTCCTTG CCATTGCCCC CACCTCTCTT ACCTTCCTCG 
GAACCCCCAC CTGCCCCACC TCTTGACCCC AAATTCATGA AAGACACCAG GCCGCCTTTC 
ACAAATTCTG GCCAGCCAGA ATCCTCCCGG GGATCCTTGA GGCCGCCTTC TACCAAGGAG 
GAGACCAGCA GGCCCCCCAT GCCCCTGATA ACCACGGAAG CATTGCAGAT GGTGCAGTTG 
AGGCCCGTGA GAAAGAACTC AGGCGCTGAG GCGGCACAGT TGTCTGAACG AACAGCTCAG 
GAACAACGAA CTCCAGTTGC TCCACAGTAC CACTTAAAGC CATCTGCTTT CCTGAAATCC 
CGAAATAGCA CAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT 
CCGACGCCTG CCAAGAGCTC GAGTCAGGGT GACCATGGCA GTGCGGCTGA GCGTGGTGGC 
CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC 
AGCACCACCC CACTCCCAGA CTCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG 
CCCAAACTGT TCCTGGTGGT ACCACCTCCG CAGAAAGATT TTGCAGTGGA GCCCGCAGAG 
AACGTGAGCG AAGCCCTCCG AGCTGTGCCC AGCCCCACGA CGGGAGAGGA GGGCTCTGTG 
CACAGCAGGG AGGCAAAAGA GAGTTCTGCA GCCCAAGCTG GCTCTCATGC CACGCACCCT 
GGCACCTCGG TTCTTGAGGG AGGAGCTGCA GGATCCATGT CTCCCAGCAG AGTGGAAGCC 
AATGTCCCCA TGGTTCAGCC TGATGTCTCA CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG 
AACAGTGCGG ATACTGGGGG CGATGGGGAG AGCTGCCTAT CTCAACAGGA CGGAGCAGCT 
GGGGTGCCGG AGACCAACGC AGCCGGTTCA TCCTCAGAGG CCTGTGACTT CCTCAAGGAA 
GACGGGAATG ATGAGGTAAT GACCCCCAGT CGACCCAGGA CCACAGAAGA CCTTTTTGCA 
GCTATTCACA GATCCAAAAG GAAAGTCCTC GGCCGTAGAG ATTCAGATGA TGACCACTCC 
CGAAACCATT CTCCCTCCCC GCCCGTGACA CCCACCGGCG CTGCCCCAAG CCTGGCCTCT 
CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC ATCCGAAAGA GCAGCACCAG CAGTGACAAC 
TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT CGTTCAGACA CCAGCGCCCG CATGTCTGCA 
GCAGAGATGC TCAAGAACAC AGACCCTAGA TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA 
GATGCCCCCG AGAGCCCGTC AAGCTGCTCC CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG 
TGGGCCAAGA ACGAAGGCTT GATGCCTCGG AGTCTGTCCT TTTCCGGCCC CAGGTACGGC 
CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC AGCAGGTACA GCATGCGGAA CCGGATCCAG 
AGCAGCCCCA TGACCGTCAT CTCGGAGGGA GAAGGGGAAG CCGTGGAGCC TGTGGACAGC 
ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG GGATGTTCCC TGGACGGACT GGCGAGGGAG 
GAGATGGACG AGGGCGGCCT GCTCTGTGGG GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG 
GCCCCCGGCC CTGTGGATGG GACAGCCAGT GCAGAGGGCA GAGAGCCCTC CCCACAGTGT 
GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535969.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534271.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu70400
Clone ID Related Accession (Same CDS sequence)	XM_011535969.2
Accession Version	XM_011535969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAA TATATTGTCT GGAGTCTGTG ACCGATTCCT CTTTCCCGTG GGGTGCAGTT 
GCGGTTTCCA ACCTAGATGA GGAAAGCCGA TGGACAGTCC ACTACACTGC CCCGTGGCAC 
CAGCAGGAGA ATGTGTTCCT TCCCACCACA AGACCCCCCT GTGTTGAGGA CCTGCACCGC 
CAAGCCAAGC TCAACCTCAA ATCAGTACTG AGGGTGATTC CAGAAGATGA GATGGGAGAC 
CAGAATTCTG GTTGCAGTGT TGTCAGTGAA TGCGATAAGT TGCGGCATGA TGGCTACCGC 
AGTTCTCAGT ACTACTCTCA GGGACCCACC TTTGCGGCCA ACGCCAGCCC ATTCTGTGAT 
GATTACCAAG ATGAAGATGA AGAAACAGAT CAAAAGTGTT CTCTGTCTTC ATCAGAAGAA 
GAAAGATTTA TTTCCATCAG GAGACCTAAA ACACCAGCCT CAAGTGACTT CTCCGACCTT 
AATACTCAGA CGAACTGGAC CAAGTCGCTT CCACTGCCAA CACCAGAAGA GAAGATGCGA 
CAGCAAGCCC AAACAGTCCA GGCTGACGTG GTGCCTATTA ACATAACTGG GGAGAATTTC 
GATCGCCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACTATTACA GGAGTCCCTG ACAACATACA GAAGGAGCTA 
GCATCAGGCA CTGGCCAAGA TGATGCTGAT GGCCACTCAG TGTACACCCC TGATCACTAC 
TCTACACTAG GAAGGTTCAA TAGCTGTCGG TCTGCTGGGC AGCGCTCAGA AACCAGGGAC 
TCCAGCTGTC AGACGGAGGA TGTGAAGGTC GTACCACCTT CCATGAGGAG AATCAGGGCA 
CAGAAGGGGC AAGGCATTGC TGCCCAGATG GGCCACTTCT CAGGTTCCTC TGGCAACATG 
TCTGTGCTGA GCGATTCTGC AGGGATCGTA TTCCCTTCCC GCCTTGACAG TGATGCTGGC 
TTCCATAGTC TTCCGCGTTC TGGAGCAAGG GCAAACATTC AGTCCCTTGA GCCGAGGCTG 
GGTGCCCTCG GCCCTGCAGG AGACATGAAT GGCACTTTCC TCTACCAGAG AGGTCACCCA 
CAAGCAGATG AAAACTTAGG CCATTTAGGA GGTGCCTCAG GGACTGGAAC ACTTTTGAGA 
CCCAAATCCC AGGAGTTGAG ACACTTCGAG AGTGAAAATA TAATGAGCCC AGCGTGTGTG 
GTTTCTCCTC ATGCAACCTA CTCCACCAGC ATCATCCCAA ATGCCACACT GTCTTCCTCT 
TCCGAGGTCA TCGCTATTCC CACTGCTCAG AGTGCGGGAC AGCGGGAAAG TAAAAGTTCT 
GGCTCATCAC ATGCAAGGAT AAAATCCAGA GACCACCTCA TCTCCAGGCA TGCTGTGAAA 
GGTGATCCTC AGTCTCCCGG TCGCCACTGG AATGAGGGCC ATGCCACCAT TCTTTCACAG 
GACTTAGACC CTCATTCCCC TGGTGAACCC GCACTGTTGT CCCTCTGTGA CTCAGCCGTC 
CCTCTAAATG CTCCAGCAAA TAGGGAGAAT GGGTCCCAAG CTATGCCGTA TAATTGTAGA 
AACAACCTGG CCTTCCCAGC CCACCCCCAA GATGTGGATG GCAAGAGTGA ATCTAGTTAT 
TCAGGGGGCG GAGGGCACAG CAGCTCGGAG CCCTGGGAAT ACAAATCCTC AGGTAATGGA 
AGGGCATCCC CCCTGAAGCC GCATTTAGCA ACTCCTGGCT ATTCCACTCC CACAAGTAAC 
ATGAGCAGCT GCAGTTTGGA CCAAACGTCC AACAAAGAGG ATGCTGGGTC GCTGTATTCT 
GAGGACCACG ATGGCTACTG TGCATCTGTG CACACTGACT CTGGACATGG ATCTGGGAAT 
CTGTGCAATA GCAGTGATGG CTTTGGGAAC CCCAGGCACA GCGTGATCAA TGTTTTTGTT 
GGAAGAGCTC AGAAAAACCA AGGGGACCGG TCCAATTACC AGGATAAATC CCTATCAAGA 
AACATCTCTT TGAAGAAAGC AAAGAAGCCT CCCCTGCCAC CCTCCCGGAC AGACTCCCTC 
CGCAGGATTC CCAAGAAGAG CAGCCAGTGC AACGGGCAGG TGCTCAACGA GAGCCTGATC 
GCCACACTCC AGCACTCGCT GCAGCTGAGC CTCCCAGGCA AGAGTGGCAG CTCGCCCTCC 
CAGAGCCCCT GCAGTGACTT GGAAGAGCCC TGGCTGCCCC GCTCCCGGAG CCAGAGCACA 
GTTAGTGCTG GCAGCAGCAT GACTTCCGCC ACCACCCCCA ATGTCTACTC CCTGTGCGGG 
GCCACGCCAT CGCAGAGTGA CACAAGCAGC GTCAAGTCAG AGTACACGGA CCCCTGGGGT 
TATTACATTG ACTACACGGG CATGCAGGAA GATCCGGGGA ACCCGGCAGG GGGCTGTTCA 
ACCAGCAGTG GGGTGCCCAC TGGGAACGGG CCAGTCCGCC ATGTCCAAGA AGGGTCCAGA 
GCCACAATGC CCCAAGTGCC CGGTGGTTCA GTCAAACCAA AGATCATGTC ACCAGAGAAG 
TCACACAGAG TCATTTCTCC ATCCAGTGGG TATTCCAGCC AGTCGAATAC ACCCACAGCA 
CTCACCCCTG TGCCTGTGTT TTTAAAATCA GTGTCACCAG CAAACGGGAA GGGGAAGCCC 
AAGCCCAAGG TACCAGAAAG GAAGTCCTCT CTGATATCTT CAGTATCCAT TTCCTCATCG 
TCCACTTCTC TTTCTTCTAG TACTTCTACT GAAGGAAGTG GCACTATGAA GAAGCTGGAT 
CCAGCCGTGG GCTCTCCCCC GGCTCCTCCT CCTCCTCCTG TTCCCTCTCC TCCATTCCCT 
TGTCCTGCAG ACAGGTCTCC TTTCCTTCCT CCCCCACCTC CTGTCACAGA TTGCTCCCAG 
GGCTCTCCTC TGCCTCACTC TCCTGTGTTC CCCCCTCCGC CGCCAGAAGC TCTCATTCCT 
TTCTGCTCCC CACCTGATTG GTGCCTTTCT CCTCCCCGCC CTGCACTGAG CCCCATTCTT 
CCAGATTCAC CTGTGTCCTT GCCATTGCCC CCACCTCTCT TACCTTCCTC GGAACCCCCA 
CCTGCCCCAC CTCTTGACCC CAAATTCATG AAAGACACCA GGCCGCCTTT CACAAATTCT 
GGCCAGCCAG AATCCTCCCG GGGATCCTTG AGGCCGCCTT CTACCAAGGA GGAGACCAGC 
AGGCCCCCCA TGCCCCTGAT AACCACGGAA GCATTGCAGA TGGTGCAGTT GAGGCCCGTG 
AGAAAGAACT CAGGCGCTGA GGCGGCACAG TTGTCTGAAC GAACAGCTCA GGAACAACGA 
ACTCCAGTTG CTCCACAGTA CCACTTAAAG CCATCTGCTT TCCTGAAATC CCGAAATAGC 
ACAAATGAAA TGGAGAGTGA AAGCCAGCCT GCCTCTGTGA CAAGCTCGCT TCCGACGCCT 
GCCAAGAGCT CGAGTCAGGG TGACCATGGC AGTGCGGCTG AGCGTGGTGG CCCTGTGAGC 
CGCAGCCCTG GAGCTCCAAG CGCTGGGGAG GCAGAGGCTC GGCCCAGCCC CAGCACCACC 
CCACTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TTTCCAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAAAGAT TTTGCAGTGG AGCCCGCAGA GAACGTGAGC 
GAAGCCCTCC GAGCTGTGCC CAGCCCCACG ACGGGAGAGG AGGGCTCTGT GCACAGCAGG 
GAGGCAAAAG AGAGTTCTGC AGCCCAAGCT GGCTCTCATG CCACGCACCC TGGCACCTCG 
GTTCTTGAGG GAGGAGCTGC AGGATCCATG TCTCCCAGCA GAGTGGAAGC CAATGTCCCC 
ATGGTTCAGC CTGATGTCTC ACCAGCCCCC AAGCAGGAGG AGCCAGCCGA GAACAGTGCG 
GATACTGGGG GCGATGGGGA GAGCTGCCTA TCTCAACAGG ACGGAGCAGC TGGGGTGCCG 
GAGACCAACG CAGCCGGTTC ATCCTCAGAG GCCTGTGACT TCCTCAAGGA AGACGGGAAT 
GATGAGGTAA TGACCCCCAG TCGACCCAGG ACCACAGAAG ACCTTTTTGC AGCTATTCAC 
AGATCCAAAA GGAAAGTCCT CGGCCGTAGA GATTCAGATG ATGACCACTC CCGAAACCAT 
TCTCCCTCCC CGCCCGTGAC ACCCACCGGC GCTGCCCCAA GCCTGGCCTC TCCAAAGCAA 
GTGGGGTCGA TTCAGAGAAG CATCCGAAAG AGCAGCACCA GCAGTGACAA CTTCAAAGCT 
CTGCTGCTGA AAAAGGGCAG TCGTTCAGAC ACCAGCGCCC GCATGTCTGC AGCAGAGATG 
CTCAAGAACA CAGACCCTAG ATTCCAGAGG TCGAGGTCAG AGCCTTCCCC AGATGCCCCC 
GAGAGCCCGT CAAGCTGCTC CCCAAGCAAG AACAGAAGGG CGCAGGAGGA GTGGGCCAAG 
AACGAAGGCT TGATGCCTCG GAGTCTGTCC TTTTCCGGCC CCAGGTACGG CCGCAGCCGA 
ACGCCGCCTT CTGCCGCCAG CAGCAGGTAC AGCATGCGGA ACCGGATCCA GAGCAGCCCC 
ATGACCGTCA TCTCGGAGGG AGAAGGGGAA GCCGTGGAGC CTGTGGACAG CATAGCCCGC 
GGGGCTCTGG GCGCTGCGGA GGGATGTTCC CTGGACGGAC TGGCGAGGGA GGAGATGGAC 
GAGGGCGGCC TGCTCTGTGG GGAGGGGCCT GCCGCCTCCC TGCAGCCCCA GGCCCCCGGC 
CCTGTGGATG GGACAGCCAG TGCAGAGGGC AGAGAGCCCT CCCCACAGTG TGGCGGTTCT 
CTGAGCGAGG AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534271.1
CDS	1740..6617
Translation	MNVIYCLESVTDSSFPWGAVAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535969.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535969.2

ORF Insert Sequence:

ATGAATGTAA TATATTGTCT GGAGTCTGTG ACCGATTCCT CTTTCCCGTG GGGTGCAGTT 
GCGGTTTCCA ACCTAGATGA GGAAAGCCGA TGGACAGTCC ACTACACTGC CCCGTGGCAC 
CAGCAGGAGA ATGTGTTCCT TCCCACCACA AGACCCCCCT GTGTTGAGGA CCTGCACCGC 
CAAGCCAAGC TCAACCTCAA ATCAGTACTG AGGGTGATTC CAGAAGATGA GATGGGAGAC 
CAGAATTCTG GTTGCAGTGT TGTCAGTGAA TGCGATAAGT TGCGGCATGA TGGCTACCGC 
AGTTCTCAGT ACTACTCTCA GGGACCCACC TTTGCGGCCA ACGCCAGCCC ATTCTGTGAT 
GATTACCAAG ATGAAGATGA AGAAACAGAT CAAAAGTGTT CTCTGTCTTC ATCAGAAGAA 
GAAAGATTTA TTTCCATCAG GAGACCTAAA ACACCAGCCT CAAGTGACTT CTCCGACCTT 
AATACTCAGA CGAACTGGAC CAAGTCGCTT CCACTGCCAA CACCAGAAGA GAAGATGCGA 
CAGCAAGCCC AAACAGTCCA GGCTGACGTG GTGCCTATTA ACATAACTGG GGAGAATTTC 
GATCGCCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACAG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACTATTACA GGAGTCCCTG ACAACATACA GAAGGAGCTA 
GCATCAGGCA CTGGCCAAGA TGATGCTGAT GGCCACTCAG TGTACACCCC TGATCACTAC 
TCTACACTAG GAAGGTTCAA TAGCTGTCGG TCTGCTGGGC AGCGCTCAGA AACCAGGGAC 
TCCAGCTGTC AGACGGAGGA TGTGAAGGTC GTACCACCTT CCATGAGGAG AATCAGGGCA 
CAGAAGGGGC AAGGCATTGC TGCCCAGATG GGCCACTTCT CAGGTTCCTC TGGCAACATG 
TCTGTGCTGA GCGATTCTGC AGGGATCGTA TTCCCTTCCC GCCTTGACAG TGATGCTGGC 
TTCCATAGTC TTCCGCGTTC TGGAGCAAGG GCAAACATTC AGTCCCTTGA GCCGAGGCTG 
GGTGCCCTCG GCCCTGCAGG AGACATGAAT GGCACTTTCC TCTACCAGAG AGGTCACCCA 
CAAGCAGATG AAAACTTAGG CCATTTAGGA GGTGCCTCAG GGACTGGAAC ACTTTTGAGA 
CCCAAATCCC AGGAGTTGAG ACACTTCGAG AGTGAAAATA TAATGAGCCC AGCGTGTGTG 
GTTTCTCCTC ATGCAACCTA CTCCACCAGC ATCATCCCAA ATGCCACACT GTCTTCCTCT 
TCCGAGGTCA TCGCTATTCC CACTGCTCAG AGTGCGGGAC AGCGGGAAAG TAAAAGTTCT 
GGCTCATCAC ATGCAAGGAT AAAATCCAGA GACCACCTCA TCTCCAGGCA TGCTGTGAAA 
GGTGATCCTC AGTCTCCCGG TCGCCACTGG AATGAGGGCC ATGCCACCAT TCTTTCACAG 
GACTTAGACC CTCATTCCCC TGGTGAACCC GCACTGTTGT CCCTCTGTGA CTCAGCCGTC 
CCTCTAAATG CTCCAGCAAA TAGGGAGAAT GGGTCCCAAG CTATGCCGTA TAATTGTAGA 
AACAACCTGG CCTTCCCAGC CCACCCCCAA GATGTGGATG GCAAGAGTGA ATCTAGTTAT 
TCAGGGGGCG GAGGGCACAG CAGCTCGGAG CCCTGGGAAT ACAAATCCTC AGGTAATGGA 
AGGGCATCCC CCCTGAAGCC GCATTTAGCA ACTCCTGGCT ATTCCACTCC CACAAGTAAC 
ATGAGCAGCT GCAGTTTGGA CCAAACGTCC AACAAAGAGG ATGCTGGGTC GCTGTATTCT 
GAGGACCACG ATGGCTACTG TGCATCTGTG CACACTGACT CTGGACATGG ATCTGGGAAT 
CTGTGCAATA GCAGTGATGG CTTTGGGAAC CCCAGGCACA GCGTGATCAA TGTTTTTGTT 
GGAAGAGCTC AGAAAAACCA AGGGGACCGG TCCAATTACC AGGATAAATC CCTATCAAGA 
AACATCTCTT TGAAGAAAGC AAAGAAGCCT CCCCTGCCAC CCTCCCGGAC AGACTCCCTC 
CGCAGGATTC CCAAGAAGAG CAGCCAGTGC AACGGGCAGG TGCTCAACGA GAGCCTGATC 
GCCACACTCC AGCACTCGCT GCAGCTGAGC CTCCCAGGCA AGAGTGGCAG CTCGCCCTCC 
CAGAGCCCCT GCAGTGACTT GGAAGAGCCC TGGCTGCCCC GCTCCCGGAG CCAGAGCACA 
GTTAGTGCTG GCAGCAGCAT GACTTCCGCC ACCACCCCCA ATGTCTACTC CCTGTGCGGG 
GCCACGCCAT CGCAGAGTGA CACAAGCAGC GTCAAGTCAG AGTACACGGA CCCCTGGGGT 
TATTACATTG ACTACACGGG CATGCAGGAA GATCCGGGGA ACCCGGCAGG GGGCTGTTCA 
ACCAGCAGTG GGGTGCCCAC TGGGAACGGG CCAGTCCGCC ATGTCCAAGA AGGGTCCAGA 
GCCACAATGC CCCAAGTGCC CGGTGGTTCA GTCAAACCAA AGATCATGTC ACCAGAGAAG 
TCACACAGAG TCATTTCTCC ATCCAGTGGG TATTCCAGCC AGTCGAATAC ACCCACAGCA 
CTCACCCCTG TGCCTGTGTT TTTAAAATCA GTGTCACCAG CAAACGGGAA GGGGAAGCCC 
AAGCCCAAGG TACCAGAAAG GAAGTCCTCT CTGATATCTT CAGTATCCAT TTCCTCATCG 
TCCACTTCTC TTTCTTCTAG TACTTCTACT GAAGGAAGTG GCACTATGAA GAAGCTGGAT 
CCAGCCGTGG GCTCTCCCCC GGCTCCTCCT CCTCCTCCTG TTCCCTCTCC TCCATTCCCT 
TGTCCTGCAG ACAGGTCTCC TTTCCTTCCT CCCCCACCTC CTGTCACAGA TTGCTCCCAG 
GGCTCTCCTC TGCCTCACTC TCCTGTGTTC CCCCCTCCGC CGCCAGAAGC TCTCATTCCT 
TTCTGCTCCC CACCTGATTG GTGCCTTTCT CCTCCCCGCC CTGCACTGAG CCCCATTCTT 
CCAGATTCAC CTGTGTCCTT GCCATTGCCC CCACCTCTCT TACCTTCCTC GGAACCCCCA 
CCTGCCCCAC CTCTTGACCC CAAATTCATG AAAGACACCA GGCCGCCTTT CACAAATTCT 
GGCCAGCCAG AATCCTCCCG GGGATCCTTG AGGCCGCCTT CTACCAAGGA GGAGACCAGC 
AGGCCCCCCA TGCCCCTGAT AACCACGGAA GCATTGCAGA TGGTGCAGTT GAGGCCCGTG 
AGAAAGAACT CAGGCGCTGA GGCGGCACAG TTGTCTGAAC GAACAGCTCA GGAACAACGA 
ACTCCAGTTG CTCCACAGTA CCACTTAAAG CCATCTGCTT TCCTGAAATC CCGAAATAGC 
ACAAATGAAA TGGAGAGTGA AAGCCAGCCT GCCTCTGTGA CAAGCTCGCT TCCGACGCCT 
GCCAAGAGCT CGAGTCAGGG TGACCATGGC AGTGCGGCTG AGCGTGGTGG CCCTGTGAGC 
CGCAGCCCTG GAGCTCCAAG CGCTGGGGAG GCAGAGGCTC GGCCCAGCCC CAGCACCACC 
CCACTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TTTCCAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAAAGAT TTTGCAGTGG AGCCCGCAGA GAACGTGAGC 
GAAGCCCTCC GAGCTGTGCC CAGCCCCACG ACGGGAGAGG AGGGCTCTGT GCACAGCAGG 
GAGGCAAAAG AGAGTTCTGC AGCCCAAGCT GGCTCTCATG CCACGCACCC TGGCACCTCG 
GTTCTTGAGG GAGGAGCTGC AGGATCCATG TCTCCCAGCA GAGTGGAAGC CAATGTCCCC 
ATGGTTCAGC CTGATGTCTC ACCAGCCCCC AAGCAGGAGG AGCCAGCCGA GAACAGTGCG 
GATACTGGGG GCGATGGGGA GAGCTGCCTA TCTCAACAGG ACGGAGCAGC TGGGGTGCCG 
GAGACCAACG CAGCCGGTTC ATCCTCAGAG GCCTGTGACT TCCTCAAGGA AGACGGGAAT 
GATGAGGTAA TGACCCCCAG TCGACCCAGG ACCACAGAAG ACCTTTTTGC AGCTATTCAC 
AGATCCAAAA GGAAAGTCCT CGGCCGTAGA GATTCAGATG ATGACCACTC CCGAAACCAT 
TCTCCCTCCC CGCCCGTGAC ACCCACCGGC GCTGCCCCAA GCCTGGCCTC TCCAAAGCAA 
GTGGGGTCGA TTCAGAGAAG CATCCGAAAG AGCAGCACCA GCAGTGACAA CTTCAAAGCT 
CTGCTGCTGA AAAAGGGCAG TCGTTCAGAC ACCAGCGCCC GCATGTCTGC AGCAGAGATG 
CTCAAGAACA CAGACCCTAG ATTCCAGAGG TCGAGGTCAG AGCCTTCCCC AGATGCCCCC 
GAGAGCCCGT CAAGCTGCTC CCCAAGCAAG AACAGAAGGG CGCAGGAGGA GTGGGCCAAG 
AACGAAGGCT TGATGCCTCG GAGTCTGTCC TTTTCCGGCC CCAGGTACGG CCGCAGCCGA 
ACGCCGCCTT CTGCCGCCAG CAGCAGGTAC AGCATGCGGA ACCGGATCCA GAGCAGCCCC 
ATGACCGTCA TCTCGGAGGG AGAAGGGGAA GCCGTGGAGC CTGTGGACAG CATAGCCCGC 
GGGGCTCTGG GCGCTGCGGA GGGATGTTCC CTGGACGGAC TGGCGAGGGA GGAGATGGAC 
GAGGGCGGCC TGCTCTGTGG GGAGGGGCCT GCCGCCTCCC TGCAGCCCCA GGCCCCCGGC 
CCTGTGGATG GGACAGCCAG TGCAGAGGGC AGAGAGCCCT CCCCACAGTG TGGCGGTTCT 
CTGAGCGAGG AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535970.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534272.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X8, mRNA.

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CloneID	OHu70401
Clone ID Related Accession (Same CDS sequence)	XM_011535970.2
Accession Version	XM_011535970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCT TCATTAATGC AAAGATTAAG TCTTTAATTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACC TAGATGAGGA AAGCCGATGG ACAGTCCACT ACACTGCCCC GTGGCACCAG 
CAGGAGAATG TGTTCCTTCC CACCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTACTGAGG GTGATTCCAG AAGATGAGAT GGGAGACCAG 
AATTCTGGTT GCAGTGTTGT CAGTGAATGC GATAAGTTGC GGCATGATGG CTACCGCAGT 
TCTCAGTACT ACTCTCAGGG ACCCACCTTT GCGGCCAACG CCAGCCCATT CTGTGATGAT 
TACCAAGATG AAGATGAAGA AACAGATCAA AAGTGTTCTC TGTCTTCATC AGAAGAAGAA 
AGATTTATTT CCATCAGGAG ACCTAAAACA CCAGCCTCAA GTGACTTCTC CGACCTTAAT 
ACTCAGACGA ACTGGACCAA GTCGCTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCCCAAA CAGTCCAGGC TGACGTGGTG CCTATTAACA TAACTGGGGA GAATTTCGAT 
CGCCAGGCCA GCCTTCGGCG GTCTCTAATT TACACAGACA CTCTGGTAAG ACGACCGAAG 
AAAGTCAAAA GGAGAAAGAC TATTACAGGA GTCCCTGACA ACATACAGAA GGAGCTAGCA 
TCAGGCACTG GCCAAGATGA TGCTGATGGC CACTCAGTGT ACACCCCTGA TCACTACTCT 
ACACTAGGAA GGTTCAATAG CTGTCGGTCT GCTGGGCAGC GCTCAGAAAC CAGGGACTCC 
AGCTGTCAGA CGGAGGATGT GAAGGTCGTA CCACCTTCCA TGAGGAGAAT CAGGGCACAG 
AAGGGGCAAG GCATTGCTGC CCAGATGGGC CACTTCTCAG GTTCCTCTGG CAACATGTCT 
GTGCTGAGCG ATTCTGCAGG GATCGTATTC CCTTCCCGCC TTGACAGTGA TGCTGGCTTC 
CATAGTCTTC CGCGTTCTGG AGCAAGGGCA AACATTCAGT CCCTTGAGCC GAGGCTGGGT 
GCCCTCGGCC CTGCAGGAGA CATGAATGGC ACTTTCCTCT ACCAGAGAGG TCACCCACAA 
GCAGATGAAA ACTTAGGCCA TTTAGGAGGT GCCTCAGGGA CTGGAACACT TTTGAGACCC 
AAATCCCAGG AGTTGAGACA CTTCGAGAGT GAAAATATAA TGAGCCCAGC GTGTGTGGTT 
TCTCCTCATG CAACCTACTC CACCAGCATC ATCCCAAATG CCACACTGTC TTCCTCTTCC 
GAGGTCATCG CTATTCCCAC TGCTCAGAGT GCGGGACAGC GGGAAAGTAA AAGTTCTGGC 
TCATCACATG CAAGGATAAA ATCCAGAGAC CACCTCATCT CCAGGCATGC TGTGAAAGGT 
GATCCTCAGT CTCCCGGTCG CCACTGGAAT GAGGGCCATG CCACCATTCT TTCACAGGAC 
TTAGACCCTC ATTCCCCTGG TGAACCCGCA CTGTTGTCCC TCTGTGACTC AGCCGTCCCT 
CTAAATGCTC CAGCAAATAG GGAGAATGGG TCCCAAGCTA TGCCGTATAA TTGTAGAAAC 
AACCTGGCCT TCCCAGCCCA CCCCCAAGAT GTGGATGGCA AGAGTGAATC TAGTTATTCA 
GGGGGCGGAG GGCACAGCAG CTCGGAGCCC TGGGAATACA AATCCTCAGG TAATGGAAGG 
GCATCCCCCC TGAAGCCGCA TTTAGCAACT CCTGGCTATT CCACTCCCAC AAGTAACATG 
AGCAGCTGCA GTTTGGACCA AACGTCCAAC AAAGAGGATG CTGGGTCGCT GTATTCTGAG 
GACCACGATG GCTACTGTGC ATCTGTGCAC ACTGACTCTG GACATGGATC TGGGAATCTG 
TGCAATAGCA GTGATGGCTT TGGGAACCCC AGGCACAGCG TGATCAATGT TTTTGTTGGA 
AGAGCTCAGA AAAACCAAGG GGACCGGTCC AATTACCAGG ATAAATCCCT ATCAAGAAAC 
ATCTCTTTGA AGAAAGCAAA GAAGCCTCCC CTGCCACCCT CCCGGACAGA CTCCCTCCGC 
AGGATTCCCA AGAAGAGCAG CCAGTGCAAC GGGCAGGTGC TCAACGAGAG CCTGATCGCC 
ACACTCCAGC ACTCGCTGCA GCTGAGCCTC CCAGGCAAGA GTGGCAGCTC GCCCTCCCAG 
AGCCCCTGCA GTGACTTGGA AGAGCCCTGG CTGCCCCGCT CCCGGAGCCA GAGCACAGTT 
AGTGCTGGCA GCAGCATGAC TTCCGCCACC ACCCCCAATG TCTACTCCCT GTGCGGGGCC 
ACGCCATCGC AGAGTGACAC AAGCAGCGTC AAGTCAGAGT ACACGGACCC CTGGGGTTAT 
TACATTGACT ACACGGGCAT GCAGGAAGAT CCGGGGAACC CGGCAGGGGG CTGTTCAACC 
AGCAGTGGGG TGCCCACTGG GAACGGGCCA GTCCGCCATG TCCAAGAAGG GTCCAGAGCC 
ACAATGCCCC AAGTGCCCGG TGGTTCAGTC AAACCAAAGA TCATGTCACC AGAGAAGTCA 
CACAGAGTCA TTTCTCCATC CAGTGGGTAT TCCAGCCAGT CGAATACACC CACAGCACTC 
ACCCCTGTGC CTGTGTTTTT AAAATCAGTG TCACCAGCAA ACGGGAAGGG GAAGCCCAAG 
CCCAAGGTAC CAGAAAGGAA GTCCTCTCTG ATATCTTCAG TATCCATTTC CTCATCGTCC 
ACTTCTCTTT CTTCTAGTAC TTCTACTGAA GGAAGTGGCA CTATGAAGAA GCTGGATCCA 
GCCGTGGGCT CTCCCCCGGC TCCTCCTCCT CCTCCTGTTC CCTCTCCTCC ATTCCCTTGT 
CCTGCAGACA GGTCTCCTTT CCTTCCTCCC CCACCTCCTG TCACAGATTG CTCCCAGGGC 
TCTCCTCTGC CTCACTCTCC TGTGTTCCCC CCTCCGCCGC CAGAAGCTCT CATTCCTTTC 
TGCTCCCCAC CTGATTGGTG CCTTTCTCCT CCCCGCCCTG CACTGAGCCC CATTCTTCCA 
GATTCACCTG TGTCCTTGCC ATTGCCCCCA CCTCTCTTAC CTTCCTCGGA ACCCCCACCT 
GCCCCACCTC TTGACCCCAA ATTCATGAAA GACACCAGGC CGCCTTTCAC AAATTCTGGC 
CAGCCAGAAT CCTCCCGGGG ATCCTTGAGG CCGCCTTCTA CCAAGGAGGA GACCAGCAGG 
CCCCCCATGC CCCTGATAAC CACGGAAGCA TTGCAGATGG TGCAGTTGAG GCCCGTGAGA 
AAGAACTCAG GCGCTGAGGC GGCACAGTTG TCTGAACGAA CAGCTCAGGA ACAACGAACT 
CCAGTTGCTC CACAGTACCA CTTAAAGCCA TCTGCTTTCC TGAAATCCCG AAATAGCACA 
AATGAAATGG AGAGTGAAAG CCAGCCTGCC TCTGTGACAA GCTCGCTTCC GACGCCTGCC 
AAGAGCTCGA GTCAGGGTGA CCATGGCAGT GCGGCTGAGC GTGGTGGCCC TGTGAGCCGC 
AGCCCTGGAG CTCCAAGCGC TGGGGAGGCA GAGGCTCGGC CCAGCCCCAG CACCACCCCA 
CTCCCAGACT CTTCACCCAG CAGGAAGCCA CCCCCCATTT CCAAGAAGCC CAAACTGTTC 
CTGGTGGTAC CACCTCCGCA GAAAGATTTT GCAGTGGAGC CCGCAGAGAA CGTGAGCGAA 
GCCCTCCGAG CTGTGCCCAG CCCCACGACG GGAGAGGAGG GCTCTGTGCA CAGCAGGGAG 
GCAAAAGAGA GTTCTGCAGC CCAAGCTGGC TCTCATGCCA CGCACCCTGG CACCTCGGTT 
CTTGAGGGAG GAGCTGCAGG ATCCATGTCT CCCAGCAGAG TGGAAGCCAA TGTCCCCATG 
GTTCAGCCTG ATGTCTCACC AGCCCCCAAG CAGGAGGAGC CAGCCGAGAA CAGTGCGGAT 
ACTGGGGGCG ATGGGGAGAG CTGCCTATCT CAACAGGACG GAGCAGCTGG GGTGCCGGAG 
ACCAACGCAG CCGGTTCATC CTCAGAGGCC TGTGACTTCC TCAAGGAAGA CGGGAATGAT 
GAGGTAATGA CCCCCAGTCG ACCCAGGACC ACAGAAGACC TTTTTGCAGC TATTCACAGA 
TCCAAAAGGA AAGTCCTCGG CCGTAGAGAT TCAGATGATG ACCACTCCCG AAACCATTCT 
CCCTCCCCGC CCGTGACACC CACCGGCGCT GCCCCAAGCC TGGCCTCTCC AAAGCAAGTG 
GGGTCGATTC AGAGAAGCAT CCGAAAGAGC AGCACCAGCA GTGACAACTT CAAAGCTCTG 
CTGCTGAAAA AGGGCAGTCG TTCAGACACC AGCGCCCGCA TGTCTGCAGC AGAGATGCTC 
AAGAACACAG ACCCTAGATT CCAGAGGTCG AGGTCAGAGC CTTCCCCAGA TGCCCCCGAG 
AGCCCGTCAA GCTGCTCCCC AAGCAAGAAC AGAAGGGCGC AGGAGGAGTG GGCCAAGAAC 
GAAGGCTTGA TGCCTCGGAG TCTGTCCTTT TCCGGCCCCA GGTACGGCCG CAGCCGAACG 
CCGCCTTCTG CCGCCAGCAG CAGGTACAGC ATGCGGAACC GGATCCAGAG CAGCCCCATG 
ACCGTCATCT CGGAGGGAGA AGGGGAAGCC GTGGAGCCTG TGGACAGCAT AGCCCGCGGG 
GCTCTGGGCG CTGCGGAGGG ATGTTCCCTG GACGGACTGG CGAGGGAGGA GATGGACGAG 
GGCGGCCTGC TCTGTGGGGA GGGGCCTGCC GCCTCCCTGC AGCCCCAGGC CCCCGGCCCT 
GTGGATGGGA CAGCCAGTGC AGAGGGCAGA GAGCCCTCCC CACAGTGTGG CGGTTCTCTG 
AGCGAGGAGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534272.1
CDS	2607..7481
Translation	MVVFINAKIKSLIKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535970.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535970.2

ORF Insert Sequence:

ATGGTGGTCT TCATTAATGC AAAGATTAAG TCTTTAATTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACC TAGATGAGGA AAGCCGATGG ACAGTCCACT ACACTGCCCC GTGGCACCAG 
CAGGAGAATG TGTTCCTTCC CACCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCCAAGCTCA ACCTCAAATC AGTACTGAGG GTGATTCCAG AAGATGAGAT GGGAGACCAG 
AATTCTGGTT GCAGTGTTGT CAGTGAATGC GATAAGTTGC GGCATGATGG CTACCGCAGT 
TCTCAGTACT ACTCTCAGGG ACCCACCTTT GCGGCCAACG CCAGCCCATT CTGTGATGAT 
TACCAAGATG AAGATGAAGA AACAGATCAA AAGTGTTCTC TGTCTTCATC AGAAGAAGAA 
AGATTTATTT CCATCAGGAG ACCTAAAACA CCAGCCTCAA GTGACTTCTC CGACCTTAAT 
ACTCAGACGA ACTGGACCAA GTCGCTTCCA CTGCCAACAC CAGAAGAGAA GATGCGACAG 
CAAGCCCAAA CAGTCCAGGC TGACGTGGTG CCTATTAACA TAACTGGGGA GAATTTCGAT 
CGCCAGGCCA GCCTTCGGCG GTCTCTAATT TACACAGACA CTCTGGTAAG ACGACCGAAG 
AAAGTCAAAA GGAGAAAGAC TATTACAGGA GTCCCTGACA ACATACAGAA GGAGCTAGCA 
TCAGGCACTG GCCAAGATGA TGCTGATGGC CACTCAGTGT ACACCCCTGA TCACTACTCT 
ACACTAGGAA GGTTCAATAG CTGTCGGTCT GCTGGGCAGC GCTCAGAAAC CAGGGACTCC 
AGCTGTCAGA CGGAGGATGT GAAGGTCGTA CCACCTTCCA TGAGGAGAAT CAGGGCACAG 
AAGGGGCAAG GCATTGCTGC CCAGATGGGC CACTTCTCAG GTTCCTCTGG CAACATGTCT 
GTGCTGAGCG ATTCTGCAGG GATCGTATTC CCTTCCCGCC TTGACAGTGA TGCTGGCTTC 
CATAGTCTTC CGCGTTCTGG AGCAAGGGCA AACATTCAGT CCCTTGAGCC GAGGCTGGGT 
GCCCTCGGCC CTGCAGGAGA CATGAATGGC ACTTTCCTCT ACCAGAGAGG TCACCCACAA 
GCAGATGAAA ACTTAGGCCA TTTAGGAGGT GCCTCAGGGA CTGGAACACT TTTGAGACCC 
AAATCCCAGG AGTTGAGACA CTTCGAGAGT GAAAATATAA TGAGCCCAGC GTGTGTGGTT 
TCTCCTCATG CAACCTACTC CACCAGCATC ATCCCAAATG CCACACTGTC TTCCTCTTCC 
GAGGTCATCG CTATTCCCAC TGCTCAGAGT GCGGGACAGC GGGAAAGTAA AAGTTCTGGC 
TCATCACATG CAAGGATAAA ATCCAGAGAC CACCTCATCT CCAGGCATGC TGTGAAAGGT 
GATCCTCAGT CTCCCGGTCG CCACTGGAAT GAGGGCCATG CCACCATTCT TTCACAGGAC 
TTAGACCCTC ATTCCCCTGG TGAACCCGCA CTGTTGTCCC TCTGTGACTC AGCCGTCCCT 
CTAAATGCTC CAGCAAATAG GGAGAATGGG TCCCAAGCTA TGCCGTATAA TTGTAGAAAC 
AACCTGGCCT TCCCAGCCCA CCCCCAAGAT GTGGATGGCA AGAGTGAATC TAGTTATTCA 
GGGGGCGGAG GGCACAGCAG CTCGGAGCCC TGGGAATACA AATCCTCAGG TAATGGAAGG 
GCATCCCCCC TGAAGCCGCA TTTAGCAACT CCTGGCTATT CCACTCCCAC AAGTAACATG 
AGCAGCTGCA GTTTGGACCA AACGTCCAAC AAAGAGGATG CTGGGTCGCT GTATTCTGAG 
GACCACGATG GCTACTGTGC ATCTGTGCAC ACTGACTCTG GACATGGATC TGGGAATCTG 
TGCAATAGCA GTGATGGCTT TGGGAACCCC AGGCACAGCG TGATCAATGT TTTTGTTGGA 
AGAGCTCAGA AAAACCAAGG GGACCGGTCC AATTACCAGG ATAAATCCCT ATCAAGAAAC 
ATCTCTTTGA AGAAAGCAAA GAAGCCTCCC CTGCCACCCT CCCGGACAGA CTCCCTCCGC 
AGGATTCCCA AGAAGAGCAG CCAGTGCAAC GGGCAGGTGC TCAACGAGAG CCTGATCGCC 
ACACTCCAGC ACTCGCTGCA GCTGAGCCTC CCAGGCAAGA GTGGCAGCTC GCCCTCCCAG 
AGCCCCTGCA GTGACTTGGA AGAGCCCTGG CTGCCCCGCT CCCGGAGCCA GAGCACAGTT 
AGTGCTGGCA GCAGCATGAC TTCCGCCACC ACCCCCAATG TCTACTCCCT GTGCGGGGCC 
ACGCCATCGC AGAGTGACAC AAGCAGCGTC AAGTCAGAGT ACACGGACCC CTGGGGTTAT 
TACATTGACT ACACGGGCAT GCAGGAAGAT CCGGGGAACC CGGCAGGGGG CTGTTCAACC 
AGCAGTGGGG TGCCCACTGG GAACGGGCCA GTCCGCCATG TCCAAGAAGG GTCCAGAGCC 
ACAATGCCCC AAGTGCCCGG TGGTTCAGTC AAACCAAAGA TCATGTCACC AGAGAAGTCA 
CACAGAGTCA TTTCTCCATC CAGTGGGTAT TCCAGCCAGT CGAATACACC CACAGCACTC 
ACCCCTGTGC CTGTGTTTTT AAAATCAGTG TCACCAGCAA ACGGGAAGGG GAAGCCCAAG 
CCCAAGGTAC CAGAAAGGAA GTCCTCTCTG ATATCTTCAG TATCCATTTC CTCATCGTCC 
ACTTCTCTTT CTTCTAGTAC TTCTACTGAA GGAAGTGGCA CTATGAAGAA GCTGGATCCA 
GCCGTGGGCT CTCCCCCGGC TCCTCCTCCT CCTCCTGTTC CCTCTCCTCC ATTCCCTTGT 
CCTGCAGACA GGTCTCCTTT CCTTCCTCCC CCACCTCCTG TCACAGATTG CTCCCAGGGC 
TCTCCTCTGC CTCACTCTCC TGTGTTCCCC CCTCCGCCGC CAGAAGCTCT CATTCCTTTC 
TGCTCCCCAC CTGATTGGTG CCTTTCTCCT CCCCGCCCTG CACTGAGCCC CATTCTTCCA 
GATTCACCTG TGTCCTTGCC ATTGCCCCCA CCTCTCTTAC CTTCCTCGGA ACCCCCACCT 
GCCCCACCTC TTGACCCCAA ATTCATGAAA GACACCAGGC CGCCTTTCAC AAATTCTGGC 
CAGCCAGAAT CCTCCCGGGG ATCCTTGAGG CCGCCTTCTA CCAAGGAGGA GACCAGCAGG 
CCCCCCATGC CCCTGATAAC CACGGAAGCA TTGCAGATGG TGCAGTTGAG GCCCGTGAGA 
AAGAACTCAG GCGCTGAGGC GGCACAGTTG TCTGAACGAA CAGCTCAGGA ACAACGAACT 
CCAGTTGCTC CACAGTACCA CTTAAAGCCA TCTGCTTTCC TGAAATCCCG AAATAGCACA 
AATGAAATGG AGAGTGAAAG CCAGCCTGCC TCTGTGACAA GCTCGCTTCC GACGCCTGCC 
AAGAGCTCGA GTCAGGGTGA CCATGGCAGT GCGGCTGAGC GTGGTGGCCC TGTGAGCCGC 
AGCCCTGGAG CTCCAAGCGC TGGGGAGGCA GAGGCTCGGC CCAGCCCCAG CACCACCCCA 
CTCCCAGACT CTTCACCCAG CAGGAAGCCA CCCCCCATTT CCAAGAAGCC CAAACTGTTC 
CTGGTGGTAC CACCTCCGCA GAAAGATTTT GCAGTGGAGC CCGCAGAGAA CGTGAGCGAA 
GCCCTCCGAG CTGTGCCCAG CCCCACGACG GGAGAGGAGG GCTCTGTGCA CAGCAGGGAG 
GCAAAAGAGA GTTCTGCAGC CCAAGCTGGC TCTCATGCCA CGCACCCTGG CACCTCGGTT 
CTTGAGGGAG GAGCTGCAGG ATCCATGTCT CCCAGCAGAG TGGAAGCCAA TGTCCCCATG 
GTTCAGCCTG ATGTCTCACC AGCCCCCAAG CAGGAGGAGC CAGCCGAGAA CAGTGCGGAT 
ACTGGGGGCG ATGGGGAGAG CTGCCTATCT CAACAGGACG GAGCAGCTGG GGTGCCGGAG 
ACCAACGCAG CCGGTTCATC CTCAGAGGCC TGTGACTTCC TCAAGGAAGA CGGGAATGAT 
GAGGTAATGA CCCCCAGTCG ACCCAGGACC ACAGAAGACC TTTTTGCAGC TATTCACAGA 
TCCAAAAGGA AAGTCCTCGG CCGTAGAGAT TCAGATGATG ACCACTCCCG AAACCATTCT 
CCCTCCCCGC CCGTGACACC CACCGGCGCT GCCCCAAGCC TGGCCTCTCC AAAGCAAGTG 
GGGTCGATTC AGAGAAGCAT CCGAAAGAGC AGCACCAGCA GTGACAACTT CAAAGCTCTG 
CTGCTGAAAA AGGGCAGTCG TTCAGACACC AGCGCCCGCA TGTCTGCAGC AGAGATGCTC 
AAGAACACAG ACCCTAGATT CCAGAGGTCG AGGTCAGAGC CTTCCCCAGA TGCCCCCGAG 
AGCCCGTCAA GCTGCTCCCC AAGCAAGAAC AGAAGGGCGC AGGAGGAGTG GGCCAAGAAC 
GAAGGCTTGA TGCCTCGGAG TCTGTCCTTT TCCGGCCCCA GGTACGGCCG CAGCCGAACG 
CCGCCTTCTG CCGCCAGCAG CAGGTACAGC ATGCGGAACC GGATCCAGAG CAGCCCCATG 
ACCGTCATCT CGGAGGGAGA AGGGGAAGCC GTGGAGCCTG TGGACAGCAT AGCCCGCGGG 
GCTCTGGGCG CTGCGGAGGG ATGTTCCCTG GACGGACTGG CGAGGGAGGA GATGGACGAG 
GGCGGCCTGC TCTGTGGGGA GGGGCCTGCC GCCTCCCTGC AGCCCCAGGC CCCCGGCCCT 
GTGGATGGGA CAGCCAGTGC AGAGGGCAGA GAGCCCTCCC CACAGTGTGG CGGTTCTCTG 
AGCGAGGAGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535971.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534273.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X10, mRNA.

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CloneID	OHu70402
Clone ID Related Accession (Same CDS sequence)	XM_011535971.2
Accession Version	XM_011535971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGGA GCTCATACTC ATACACGTCA ACACTACCCA TGACCGGAAA AATGACAACT 
GGCCTACCGA ACTGGTCCTT CTGGCTTTTC ACACTGCAGT GCTTGTTTGA AATACAGTTG 
ACCCTTGAAC ACAACACATT TGAACTTGGA GGGTCCACTT ATATGGATTT TTTCAATACA 
GTCAGCCCTC CATACATTCT GGGTCCTGCA ACCGAAACCA AATGCGAATG GAAAATACAA 
TACTCAGAAT GCGATAAGTT GCGGCATGAT GGCTACCGCA GTTCTCAGTA CTACTCTCAG 
GGACCCACCT TTGCGGCCAA CGCCAGCCCA TTCTGTGATG ATTACCAAGA TGAAGATGAA 
GAAACAGATC AAAAGTGTTC TCTGTCTTCA TCAGAAGAAG AAAGATTTAT TTCCATCAGG 
AGACCTAAAA CACCAGCCTC AAGTGACTTC TCCGACCTTA ATACTCAGAC GAACTGGACC 
AAGTCGCTTC CACTGCCAAC ACCAGAAGAG AAGATGCGAC AGCAAGCCCA AACAGTCCAG 
GCTGACGTGG TGCCTATTAA CATAACTGGG GAGAATTTCG ATCGCCAGGC CAGCCTTCGG 
CGGTCTCTAA TTTACACAGA CACTCTGGTA AGACGACCGA AGAAAGTCAA AAGGAGAAAG 
ACTATTACAG GAGTCCCTGA CAACATACAG AAGGAGCTAG CATCAGGCAC TGGCCAAGAT 
GATGCTGATG GCCACTCAGT GTACACCCCT GATCACTACT CTACACTAGG AAGGTTCAAT 
AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA ACCAGGGACT CCAGCTGTCA GACGGAGGAT 
GTGAAGGTCG TACCACCTTC CATGAGGAGA ATCAGGGCAC AGAAGGGGCA AGGCATTGCT 
GCCCAGATGG GCCACTTCTC AGGTTCCTCT GGCAACATGT CTGTGCTGAG CGATTCTGCA 
GGGATCGTAT TCCCTTCCCG CCTTGACAGT GATGCTGGCT TCCATAGTCT TCCGCGTTCT 
GGAGCAAGGG CAAACATTCA GTCCCTTGAG CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA 
GACATGAATG GCACTTTCCT CTACCAGAGA GGTCACCCAC AAGCAGATGA AAACTTAGGC 
CATTTAGGAG GTGCCTCAGG GACTGGAACA CTTTTGAGAC CCAAATCCCA GGAGTTGAGA 
CACTTCGAGA GTGAAAATAT AATGAGCCCA GCGTGTGTGG TTTCTCCTCA TGCAACCTAC 
TCCACCAGCA TCATCCCAAA TGCCACACTG TCTTCCTCTT CCGAGGTCAT CGCTATTCCC 
ACTGCTCAGA GTGCGGGACA GCGGGAAAGT AAAAGTTCTG GCTCATCACA TGCAAGGATA 
AAATCCAGAG ACCACCTCAT CTCCAGGCAT GCTGTGAAAG GTGATCCTCA GTCTCCCGGT 
CGCCACTGGA ATGAGGGCCA TGCCACCATT CTTTCACAGG ACTTAGACCC TCATTCCCCT 
GGTGAACCCG CACTGTTGTC CCTCTGTGAC TCAGCCGTCC CTCTAAATGC TCCAGCAAAT 
AGGGAGAATG GGTCCCAAGC TATGCCGTAT AATTGTAGAA ACAACCTGGC CTTCCCAGCC 
CACCCCCAAG ATGTGGATGG CAAGAGTGAA TCTAGTTATT CAGGGGGCGG AGGGCACAGC 
AGCTCGGAGC CCTGGGAATA CAAATCCTCA GGTAATGGAA GGGCATCCCC CCTGAAGCCG 
CATTTAGCAA CTCCTGGCTA TTCCACTCCC ACAAGTAACA TGAGCAGCTG CAGTTTGGAC 
CAAACGTCCA ACAAAGAGGA TGCTGGGTCG CTGTATTCTG AGGACCACGA TGGCTACTGT 
GCATCTGTGC ACACTGACTC TGGACATGGA TCTGGGAATC TGTGCAATAG CAGTGATGGC 
TTTGGGAACC CCAGGCACAG CGTGATCAAT GTTTTTGTTG GAAGAGCTCA GAAAAACCAA 
GGGGACCGGT CCAATTACCA GGATAAATCC CTATCAAGAA ACATCTCTTT GAAGAAAGCA 
AAGAAGCCTC CCCTGCCACC CTCCCGGACA GACTCCCTCC GCAGGATTCC CAAGAAGAGC 
AGCCAGTGCA ACGGGCAGGT GCTCAACGAG AGCCTGATCG CCACACTCCA GCACTCGCTG 
CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC TCGCCCTCCC AGAGCCCCTG CAGTGACTTG 
GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC CAGAGCACAG TTAGTGCTGG CAGCAGCATG 
ACTTCCGCCA CCACCCCCAA TGTCTACTCC CTGTGCGGGG CCACGCCATC GCAGAGTGAC 
ACAAGCAGCG TCAAGTCAGA GTACACGGAC CCCTGGGGTT ATTACATTGA CTACACGGGC 
ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG GGCTGTTCAA CCAGCAGTGG GGTGCCCACT 
GGGAACGGGC CAGTCCGCCA TGTCCAAGAA GGGTCCAGAG CCACAATGCC CCAAGTGCCC 
GGTGGTTCAG TCAAACCAAA GATCATGTCA CCAGAGAAGT CACACAGAGT CATTTCTCCA 
TCCAGTGGGT ATTCCAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAG TGTCACCAGC AAACGGGAAG GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG 
AAGTCCTCTC TGATATCTTC AGTATCCATT TCCTCATCGT CCACTTCTCT TTCTTCTAGT 
ACTTCTACTG AAGGAAGTGG CACTATGAAG AAGCTGGATC CAGCCGTGGG CTCTCCCCCG 
GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT CCATTCCCTT GTCCTGCAGA CAGGTCTCCT 
TTCCTTCCTC CCCCACCTCC TGTCACAGAT TGCTCCCAGG GCTCTCCTCT GCCTCACTCT 
CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT CTCATTCCTT TCTGCTCCCC ACCTGATTGG 
TGCCTTTCTC CTCCCCGCCC TGCACTGAGC CCCATTCTTC CAGATTCACC TGTGTCCTTG 
CCATTGCCCC CACCTCTCTT ACCTTCCTCG GAACCCCCAC CTGCCCCACC TCTTGACCCC 
AAATTCATGA AAGACACCAG GCCGCCTTTC ACAAATTCTG GCCAGCCAGA ATCCTCCCGG 
GGATCCTTGA GGCCGCCTTC TACCAAGGAG GAGACCAGCA GGCCCCCCAT GCCCCTGATA 
ACCACGGAAG CATTGCAGAT GGTGCAGTTG AGGCCCGTGA GAAAGAACTC AGGCGCTGAG 
GCGGCACAGT TGTCTGAACG AACAGCTCAG GAACAACGAA CTCCAGTTGC TCCACAGTAC 
CACTTAAAGC CATCTGCTTT CCTGAAATCC CGAAATAGCA CAAATGAAAT GGAGAGTGAA 
AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT CCGACGCCTG CCAAGAGCTC GAGTCAGGGT 
GACCATGGCA GTGCGGCTGA GCGTGGTGGC CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC 
GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC AGCACCACCC CACTCCCAGA CTCTTCACCC 
AGCAGGAAGC CACCCCCCAT TTCCAAGAAG CCCAAACTGT TCCTGGTGGT ACCACCTCCG 
CAGAAAGATT TTGCAGTGGA GCCCGCAGAG AACGTGAGCG AAGCCCTCCG AGCTGTGCCC 
AGCCCCACGA CGGGAGAGGA GGGCTCTGTG CACAGCAGGG AGGCAAAAGA GAGTTCTGCA 
GCCCAAGCTG GCTCTCATGC CACGCACCCT GGCACCTCGG TTCTTGAGGG AGGAGCTGCA 
GGATCCATGT CTCCCAGCAG AGTGGAAGCC AATGTCCCCA TGGTTCAGCC TGATGTCTCA 
CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG AACAGTGCGG ATACTGGGGG CGATGGGGAG 
AGCTGCCTAT CTCAACAGGA CGGAGCAGCT GGGGTGCCGG AGACCAACGC AGCCGGTTCA 
TCCTCAGAGG CCTGTGACTT CCTCAAGGAA GACGGGAATG ATGAGGTAAT GACCCCCAGT 
CGACCCAGGA CCACAGAAGA CCTTTTTGCA GCTATTCACA GATCCAAAAG GAAAGTCCTC 
GGCCGTAGAG ATTCAGATGA TGACCACTCC CGAAACCATT CTCCCTCCCC GCCCGTGACA 
CCCACCGGCG CTGCCCCAAG CCTGGCCTCT CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC 
ATCCGAAAGA GCAGCACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT 
CGTTCAGACA CCAGCGCCCG CATGTCTGCA GCAGAGATGC TCAAGAACAC AGACCCTAGA 
TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA GATGCCCCCG AGAGCCCGTC AAGCTGCTCC 
CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG TGGGCCAAGA ACGAAGGCTT GATGCCTCGG 
AGTCTGTCCT TTTCCGGCCC CAGGTACGGC CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC 
AGCAGGTACA GCATGCGGAA CCGGATCCAG AGCAGCCCCA TGACCGTCAT CTCGGAGGGA 
GAAGGGGAAG CCGTGGAGCC TGTGGACAGC ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG 
GGATGTTCCC TGGACGGACT GGCGAGGGAG GAGATGGACG AGGGCGGCCT GCTCTGTGGG 
GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG GCCCCCGGCC CTGTGGATGG GACAGCCAGT 
GCAGAGGGCA GAGAGCCCTC CCCACAGTGT GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534273.1
CDS	892..5748
Translation	MKGSSYSYTSTLPMTGKMTTGLPNWSFWLFTLQCLFEIQLTLEHNTFELGGSTYMDFFNTVSPPYILGPATETKCEWKIQYSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535971.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535971.2

ORF Insert Sequence:

ATGAAGGGGA GCTCATACTC ATACACGTCA ACACTACCCA TGACCGGAAA AATGACAACT 
GGCCTACCGA ACTGGTCCTT CTGGCTTTTC ACACTGCAGT GCTTGTTTGA AATACAGTTG 
ACCCTTGAAC ACAACACATT TGAACTTGGA GGGTCCACTT ATATGGATTT TTTCAATACA 
GTCAGCCCTC CATACATTCT GGGTCCTGCA ACCGAAACCA AATGCGAATG GAAAATACAA 
TACTCAGAAT GCGATAAGTT GCGGCATGAT GGCTACCGCA GTTCTCAGTA CTACTCTCAG 
GGACCCACCT TTGCGGCCAA CGCCAGCCCA TTCTGTGATG ATTACCAAGA TGAAGATGAA 
GAAACAGATC AAAAGTGTTC TCTGTCTTCA TCAGAAGAAG AAAGATTTAT TTCCATCAGG 
AGACCTAAAA CACCAGCCTC AAGTGACTTC TCCGACCTTA ATACTCAGAC GAACTGGACC 
AAGTCGCTTC CACTGCCAAC ACCAGAAGAG AAGATGCGAC AGCAAGCCCA AACAGTCCAG 
GCTGACGTGG TGCCTATTAA CATAACTGGG GAGAATTTCG ATCGCCAGGC CAGCCTTCGG 
CGGTCTCTAA TTTACACAGA CACTCTGGTA AGACGACCGA AGAAAGTCAA AAGGAGAAAG 
ACTATTACAG GAGTCCCTGA CAACATACAG AAGGAGCTAG CATCAGGCAC TGGCCAAGAT 
GATGCTGATG GCCACTCAGT GTACACCCCT GATCACTACT CTACACTAGG AAGGTTCAAT 
AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA ACCAGGGACT CCAGCTGTCA GACGGAGGAT 
GTGAAGGTCG TACCACCTTC CATGAGGAGA ATCAGGGCAC AGAAGGGGCA AGGCATTGCT 
GCCCAGATGG GCCACTTCTC AGGTTCCTCT GGCAACATGT CTGTGCTGAG CGATTCTGCA 
GGGATCGTAT TCCCTTCCCG CCTTGACAGT GATGCTGGCT TCCATAGTCT TCCGCGTTCT 
GGAGCAAGGG CAAACATTCA GTCCCTTGAG CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA 
GACATGAATG GCACTTTCCT CTACCAGAGA GGTCACCCAC AAGCAGATGA AAACTTAGGC 
CATTTAGGAG GTGCCTCAGG GACTGGAACA CTTTTGAGAC CCAAATCCCA GGAGTTGAGA 
CACTTCGAGA GTGAAAATAT AATGAGCCCA GCGTGTGTGG TTTCTCCTCA TGCAACCTAC 
TCCACCAGCA TCATCCCAAA TGCCACACTG TCTTCCTCTT CCGAGGTCAT CGCTATTCCC 
ACTGCTCAGA GTGCGGGACA GCGGGAAAGT AAAAGTTCTG GCTCATCACA TGCAAGGATA 
AAATCCAGAG ACCACCTCAT CTCCAGGCAT GCTGTGAAAG GTGATCCTCA GTCTCCCGGT 
CGCCACTGGA ATGAGGGCCA TGCCACCATT CTTTCACAGG ACTTAGACCC TCATTCCCCT 
GGTGAACCCG CACTGTTGTC CCTCTGTGAC TCAGCCGTCC CTCTAAATGC TCCAGCAAAT 
AGGGAGAATG GGTCCCAAGC TATGCCGTAT AATTGTAGAA ACAACCTGGC CTTCCCAGCC 
CACCCCCAAG ATGTGGATGG CAAGAGTGAA TCTAGTTATT CAGGGGGCGG AGGGCACAGC 
AGCTCGGAGC CCTGGGAATA CAAATCCTCA GGTAATGGAA GGGCATCCCC CCTGAAGCCG 
CATTTAGCAA CTCCTGGCTA TTCCACTCCC ACAAGTAACA TGAGCAGCTG CAGTTTGGAC 
CAAACGTCCA ACAAAGAGGA TGCTGGGTCG CTGTATTCTG AGGACCACGA TGGCTACTGT 
GCATCTGTGC ACACTGACTC TGGACATGGA TCTGGGAATC TGTGCAATAG CAGTGATGGC 
TTTGGGAACC CCAGGCACAG CGTGATCAAT GTTTTTGTTG GAAGAGCTCA GAAAAACCAA 
GGGGACCGGT CCAATTACCA GGATAAATCC CTATCAAGAA ACATCTCTTT GAAGAAAGCA 
AAGAAGCCTC CCCTGCCACC CTCCCGGACA GACTCCCTCC GCAGGATTCC CAAGAAGAGC 
AGCCAGTGCA ACGGGCAGGT GCTCAACGAG AGCCTGATCG CCACACTCCA GCACTCGCTG 
CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC TCGCCCTCCC AGAGCCCCTG CAGTGACTTG 
GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC CAGAGCACAG TTAGTGCTGG CAGCAGCATG 
ACTTCCGCCA CCACCCCCAA TGTCTACTCC CTGTGCGGGG CCACGCCATC GCAGAGTGAC 
ACAAGCAGCG TCAAGTCAGA GTACACGGAC CCCTGGGGTT ATTACATTGA CTACACGGGC 
ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG GGCTGTTCAA CCAGCAGTGG GGTGCCCACT 
GGGAACGGGC CAGTCCGCCA TGTCCAAGAA GGGTCCAGAG CCACAATGCC CCAAGTGCCC 
GGTGGTTCAG TCAAACCAAA GATCATGTCA CCAGAGAAGT CACACAGAGT CATTTCTCCA 
TCCAGTGGGT ATTCCAGCCA GTCGAATACA CCCACAGCAC TCACCCCTGT GCCTGTGTTT 
TTAAAATCAG TGTCACCAGC AAACGGGAAG GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG 
AAGTCCTCTC TGATATCTTC AGTATCCATT TCCTCATCGT CCACTTCTCT TTCTTCTAGT 
ACTTCTACTG AAGGAAGTGG CACTATGAAG AAGCTGGATC CAGCCGTGGG CTCTCCCCCG 
GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT CCATTCCCTT GTCCTGCAGA CAGGTCTCCT 
TTCCTTCCTC CCCCACCTCC TGTCACAGAT TGCTCCCAGG GCTCTCCTCT GCCTCACTCT 
CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT CTCATTCCTT TCTGCTCCCC ACCTGATTGG 
TGCCTTTCTC CTCCCCGCCC TGCACTGAGC CCCATTCTTC CAGATTCACC TGTGTCCTTG 
CCATTGCCCC CACCTCTCTT ACCTTCCTCG GAACCCCCAC CTGCCCCACC TCTTGACCCC 
AAATTCATGA AAGACACCAG GCCGCCTTTC ACAAATTCTG GCCAGCCAGA ATCCTCCCGG 
GGATCCTTGA GGCCGCCTTC TACCAAGGAG GAGACCAGCA GGCCCCCCAT GCCCCTGATA 
ACCACGGAAG CATTGCAGAT GGTGCAGTTG AGGCCCGTGA GAAAGAACTC AGGCGCTGAG 
GCGGCACAGT TGTCTGAACG AACAGCTCAG GAACAACGAA CTCCAGTTGC TCCACAGTAC 
CACTTAAAGC CATCTGCTTT CCTGAAATCC CGAAATAGCA CAAATGAAAT GGAGAGTGAA 
AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT CCGACGCCTG CCAAGAGCTC GAGTCAGGGT 
GACCATGGCA GTGCGGCTGA GCGTGGTGGC CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC 
GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC AGCACCACCC CACTCCCAGA CTCTTCACCC 
AGCAGGAAGC CACCCCCCAT TTCCAAGAAG CCCAAACTGT TCCTGGTGGT ACCACCTCCG 
CAGAAAGATT TTGCAGTGGA GCCCGCAGAG AACGTGAGCG AAGCCCTCCG AGCTGTGCCC 
AGCCCCACGA CGGGAGAGGA GGGCTCTGTG CACAGCAGGG AGGCAAAAGA GAGTTCTGCA 
GCCCAAGCTG GCTCTCATGC CACGCACCCT GGCACCTCGG TTCTTGAGGG AGGAGCTGCA 
GGATCCATGT CTCCCAGCAG AGTGGAAGCC AATGTCCCCA TGGTTCAGCC TGATGTCTCA 
CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG AACAGTGCGG ATACTGGGGG CGATGGGGAG 
AGCTGCCTAT CTCAACAGGA CGGAGCAGCT GGGGTGCCGG AGACCAACGC AGCCGGTTCA 
TCCTCAGAGG CCTGTGACTT CCTCAAGGAA GACGGGAATG ATGAGGTAAT GACCCCCAGT 
CGACCCAGGA CCACAGAAGA CCTTTTTGCA GCTATTCACA GATCCAAAAG GAAAGTCCTC 
GGCCGTAGAG ATTCAGATGA TGACCACTCC CGAAACCATT CTCCCTCCCC GCCCGTGACA 
CCCACCGGCG CTGCCCCAAG CCTGGCCTCT CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC 
ATCCGAAAGA GCAGCACCAG CAGTGACAAC TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT 
CGTTCAGACA CCAGCGCCCG CATGTCTGCA GCAGAGATGC TCAAGAACAC AGACCCTAGA 
TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA GATGCCCCCG AGAGCCCGTC AAGCTGCTCC 
CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG TGGGCCAAGA ACGAAGGCTT GATGCCTCGG 
AGTCTGTCCT TTTCCGGCCC CAGGTACGGC CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC 
AGCAGGTACA GCATGCGGAA CCGGATCCAG AGCAGCCCCA TGACCGTCAT CTCGGAGGGA 
GAAGGGGAAG CCGTGGAGCC TGTGGACAGC ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG 
GGATGTTCCC TGGACGGACT GGCGAGGGAG GAGATGGACG AGGGCGGCCT GCTCTGTGGG 
GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG GCCCCCGGCC CTGTGGATGG GACAGCCAGT 
GCAGAGGGCA GAGAGCCCTC CCCACAGTGT GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535972.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534274.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X11, mRNA.

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CloneID	OHu70403
Clone ID Related Accession (Same CDS sequence)	XM_011535972.2
Accession Version	XM_011535972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGTC TGAAAGCGGT TTCCAACCTA GATGAGGAAA GCCGATGGAC AGTCCACTAC 
ACTGCCCCGT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TACTGAGGGT GATTCCAGAA 
GATGAGATGG GAGACCAGAA TTCTGGTTGC AGTGTTGTCA GTGAATGCGA TAAGTTGCGG 
CATGATGGCT ACCGCAGTTC TCAGTACTAC TCTCAGGGAC CCACCTTTGC GGCCAACGCC 
AGCCCATTCT GTGATGATTA CCAAGATGAA GATGAAGAAA CAGATCAAAA GTGTTCTCTG 
TCTTCATCAG AAGAAGAAAG ATTTATTTCC ATCAGGAGAC CTAAAACACC AGCCTCAAGT 
GACTTCTCCG ACCTTAATAC TCAGACGAAC TGGACCAAGT CGCTTCCACT GCCAACACCA 
GAAGAGAAGA TGCGACAGCA AGCCCAAACA GTCCAGGCTG ACGTGGTGCC TATTAACATA 
ACTGGGGAGA ATTTCGATCG CCAGGCCAGC CTTCGGCGGT CTCTAATTTA CACAGACACT 
CTGGTAAGAC GACCGAAGAA AGTCAAAAGG AGAAAGACTA TTACAGGAGT CCCTGACAAC 
ATACAGAAGG AGCTAGCATC AGGCACTGGC CAAGATGATG CTGATGGCCA CTCAGTGTAC 
ACCCCTGATC ACTACTCTAC ACTAGGAAGG TTCAATAGCT GTCGGTCTGC TGGGCAGCGC 
TCAGAAACCA GGGACTCCAG CTGTCAGACG GAGGATGTGA AGGTCGTACC ACCTTCCATG 
AGGAGAATCA GGGCACAGAA GGGGCAAGGC ATTGCTGCCC AGATGGGCCA CTTCTCAGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAT TCTGCAGGGA TCGTATTCCC TTCCCGCCTT 
GACAGTGATG CTGGCTTCCA TAGTCTTCCG CGTTCTGGAG CAAGGGCAAA CATTCAGTCC 
CTTGAGCCGA GGCTGGGTGC CCTCGGCCCT GCAGGAGACA TGAATGGCAC TTTCCTCTAC 
CAGAGAGGTC ACCCACAAGC AGATGAAAAC TTAGGCCATT TAGGAGGTGC CTCAGGGACT 
GGAACACTTT TGAGACCCAA ATCCCAGGAG TTGAGACACT TCGAGAGTGA AAATATAATG 
AGCCCAGCGT GTGTGGTTTC TCCTCATGCA ACCTACTCCA CCAGCATCAT CCCAAATGCC 
ACACTGTCTT CCTCTTCCGA GGTCATCGCT ATTCCCACTG CTCAGAGTGC GGGACAGCGG 
GAAAGTAAAA GTTCTGGCTC ATCACATGCA AGGATAAAAT CCAGAGACCA CCTCATCTCC 
AGGCATGCTG TGAAAGGTGA TCCTCAGTCT CCCGGTCGCC ACTGGAATGA GGGCCATGCC 
ACCATTCTTT CACAGGACTT AGACCCTCAT TCCCCTGGTG AACCCGCACT GTTGTCCCTC 
TGTGACTCAG CCGTCCCTCT AAATGCTCCA GCAAATAGGG AGAATGGGTC CCAAGCTATG 
CCGTATAATT GTAGAAACAA CCTGGCCTTC CCAGCCCACC CCCAAGATGT GGATGGCAAG 
AGTGAATCTA GTTATTCAGG GGGCGGAGGG CACAGCAGCT CGGAGCCCTG GGAATACAAA 
TCCTCAGGTA ATGGAAGGGC ATCCCCCCTG AAGCCGCATT TAGCAACTCC TGGCTATTCC 
ACTCCCACAA GTAACATGAG CAGCTGCAGT TTGGACCAAA CGTCCAACAA AGAGGATGCT 
GGGTCGCTGT ATTCTGAGGA CCACGATGGC TACTGTGCAT CTGTGCACAC TGACTCTGGA 
CATGGATCTG GGAATCTGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGCGTG 
ATCAATGTTT TTGTTGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCCAA TTACCAGGAT 
AAATCCCTAT CAAGAAACAT CTCTTTGAAG AAAGCAAAGA AGCCTCCCCT GCCACCCTCC 
CGGACAGACT CCCTCCGCAG GATTCCCAAG AAGAGCAGCC AGTGCAACGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCAC ACTCCAGCAC TCGCTGCAGC TGAGCCTCCC AGGCAAGAGT 
GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GACTTGGAAG AGCCCTGGCT GCCCCGCTCC 
CGGAGCCAGA GCACAGTTAG TGCTGGCAGC AGCATGACTT CCGCCACCAC CCCCAATGTC 
TACTCCCTGT GCGGGGCCAC GCCATCGCAG AGTGACACAA GCAGCGTCAA GTCAGAGTAC 
ACGGACCCCT GGGGTTATTA CATTGACTAC ACGGGCATGC AGGAAGATCC GGGGAACCCG 
GCAGGGGGCT GTTCAACCAG CAGTGGGGTG CCCACTGGGA ACGGGCCAGT CCGCCATGTC 
CAAGAAGGGT CCAGAGCCAC AATGCCCCAA GTGCCCGGTG GTTCAGTCAA ACCAAAGATC 
ATGTCACCAG AGAAGTCACA CAGAGTCATT TCTCCATCCA GTGGGTATTC CAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAGTGTC ACCAGCAAAC 
GGGAAGGGGA AGCCCAAGCC CAAGGTACCA GAAAGGAAGT CCTCTCTGAT ATCTTCAGTA 
TCCATTTCCT CATCGTCCAC TTCTCTTTCT TCTAGTACTT CTACTGAAGG AAGTGGCACT 
ATGAAGAAGC TGGATCCAGC CGTGGGCTCT CCCCCGGCTC CTCCTCCTCC TCCTGTTCCC 
TCTCCTCCAT TCCCTTGTCC TGCAGACAGG TCTCCTTTCC TTCCTCCCCC ACCTCCTGTC 
ACAGATTGCT CCCAGGGCTC TCCTCTGCCT CACTCTCCTG TGTTCCCCCC TCCGCCGCCA 
GAAGCTCTCA TTCCTTTCTG CTCCCCACCT GATTGGTGCC TTTCTCCTCC CCGCCCTGCA 
CTGAGCCCCA TTCTTCCAGA TTCACCTGTG TCCTTGCCAT TGCCCCCACC TCTCTTACCT 
TCCTCGGAAC CCCCACCTGC CCCACCTCTT GACCCCAAAT TCATGAAAGA CACCAGGCCG 
CCTTTCACAA ATTCTGGCCA GCCAGAATCC TCCCGGGGAT CCTTGAGGCC GCCTTCTACC 
AAGGAGGAGA CCAGCAGGCC CCCCATGCCC CTGATAACCA CGGAAGCATT GCAGATGGTG 
CAGTTGAGGC CCGTGAGAAA GAACTCAGGC GCTGAGGCGG CACAGTTGTC TGAACGAACA 
GCTCAGGAAC AACGAACTCC AGTTGCTCCA CAGTACCACT TAAAGCCATC TGCTTTCCTG 
AAATCCCGAA ATAGCACAAA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC TGTGACAAGC 
TCGCTTCCGA CGCCTGCCAA GAGCTCGAGT CAGGGTGACC ATGGCAGTGC GGCTGAGCGT 
GGTGGCCCTG TGAGCCGCAG CCCTGGAGCT CCAAGCGCTG GGGAGGCAGA GGCTCGGCCC 
AGCCCCAGCA CCACCCCACT CCCAGACTCT TCACCCAGCA GGAAGCCACC CCCCATTTCC 
AAGAAGCCCA AACTGTTCCT GGTGGTACCA CCTCCGCAGA AAGATTTTGC AGTGGAGCCC 
GCAGAGAACG TGAGCGAAGC CCTCCGAGCT GTGCCCAGCC CCACGACGGG AGAGGAGGGC 
TCTGTGCACA GCAGGGAGGC AAAAGAGAGT TCTGCAGCCC AAGCTGGCTC TCATGCCACG 
CACCCTGGCA CCTCGGTTCT TGAGGGAGGA GCTGCAGGAT CCATGTCTCC CAGCAGAGTG 
GAAGCCAATG TCCCCATGGT TCAGCCTGAT GTCTCACCAG CCCCCAAGCA GGAGGAGCCA 
GCCGAGAACA GTGCGGATAC TGGGGGCGAT GGGGAGAGCT GCCTATCTCA ACAGGACGGA 
GCAGCTGGGG TGCCGGAGAC CAACGCAGCC GGTTCATCCT CAGAGGCCTG TGACTTCCTC 
AAGGAAGACG GGAATGATGA GGTAATGACC CCCAGTCGAC CCAGGACCAC AGAAGACCTT 
TTTGCAGCTA TTCACAGATC CAAAAGGAAA GTCCTCGGCC GTAGAGATTC AGATGATGAC 
CACTCCCGAA ACCATTCTCC CTCCCCGCCC GTGACACCCA CCGGCGCTGC CCCAAGCCTG 
GCCTCTCCAA AGCAAGTGGG GTCGATTCAG AGAAGCATCC GAAAGAGCAG CACCAGCAGT 
GACAACTTCA AAGCTCTGCT GCTGAAAAAG GGCAGTCGTT CAGACACCAG CGCCCGCATG 
TCTGCAGCAG AGATGCTCAA GAACACAGAC CCTAGATTCC AGAGGTCGAG GTCAGAGCCT 
TCCCCAGATG CCCCCGAGAG CCCGTCAAGC TGCTCCCCAA GCAAGAACAG AAGGGCGCAG 
GAGGAGTGGG CCAAGAACGA AGGCTTGATG CCTCGGAGTC TGTCCTTTTC CGGCCCCAGG 
TACGGCCGCA GCCGAACGCC GCCTTCTGCC GCCAGCAGCA GGTACAGCAT GCGGAACCGG 
ATCCAGAGCA GCCCCATGAC CGTCATCTCG GAGGGAGAAG GGGAAGCCGT GGAGCCTGTG 
GACAGCATAG CCCGCGGGGC TCTGGGCGCT GCGGAGGGAT GTTCCCTGGA CGGACTGGCG 
AGGGAGGAGA TGGACGAGGG CGGCCTGCTC TGTGGGGAGG GGCCTGCCGC CTCCCTGCAG 
CCCCAGGCCC CCGGCCCTGT GGATGGGACA GCCAGTGCAG AGGGCAGAGA GCCCTCCCCA 
CAGTGTGGCG GTTCTCTGAG CGAGGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534274.1
CDS	730..5562
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535972.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535972.2

ORF Insert Sequence:

ATGTTCTGTC TGAAAGCGGT TTCCAACCTA GATGAGGAAA GCCGATGGAC AGTCCACTAC 
ACTGCCCCGT GGCACCAGCA GGAGAATGTG TTCCTTCCCA CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC CAAGCTCAAC CTCAAATCAG TACTGAGGGT GATTCCAGAA 
GATGAGATGG GAGACCAGAA TTCTGGTTGC AGTGTTGTCA GTGAATGCGA TAAGTTGCGG 
CATGATGGCT ACCGCAGTTC TCAGTACTAC TCTCAGGGAC CCACCTTTGC GGCCAACGCC 
AGCCCATTCT GTGATGATTA CCAAGATGAA GATGAAGAAA CAGATCAAAA GTGTTCTCTG 
TCTTCATCAG AAGAAGAAAG ATTTATTTCC ATCAGGAGAC CTAAAACACC AGCCTCAAGT 
GACTTCTCCG ACCTTAATAC TCAGACGAAC TGGACCAAGT CGCTTCCACT GCCAACACCA 
GAAGAGAAGA TGCGACAGCA AGCCCAAACA GTCCAGGCTG ACGTGGTGCC TATTAACATA 
ACTGGGGAGA ATTTCGATCG CCAGGCCAGC CTTCGGCGGT CTCTAATTTA CACAGACACT 
CTGGTAAGAC GACCGAAGAA AGTCAAAAGG AGAAAGACTA TTACAGGAGT CCCTGACAAC 
ATACAGAAGG AGCTAGCATC AGGCACTGGC CAAGATGATG CTGATGGCCA CTCAGTGTAC 
ACCCCTGATC ACTACTCTAC ACTAGGAAGG TTCAATAGCT GTCGGTCTGC TGGGCAGCGC 
TCAGAAACCA GGGACTCCAG CTGTCAGACG GAGGATGTGA AGGTCGTACC ACCTTCCATG 
AGGAGAATCA GGGCACAGAA GGGGCAAGGC ATTGCTGCCC AGATGGGCCA CTTCTCAGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAT TCTGCAGGGA TCGTATTCCC TTCCCGCCTT 
GACAGTGATG CTGGCTTCCA TAGTCTTCCG CGTTCTGGAG CAAGGGCAAA CATTCAGTCC 
CTTGAGCCGA GGCTGGGTGC CCTCGGCCCT GCAGGAGACA TGAATGGCAC TTTCCTCTAC 
CAGAGAGGTC ACCCACAAGC AGATGAAAAC TTAGGCCATT TAGGAGGTGC CTCAGGGACT 
GGAACACTTT TGAGACCCAA ATCCCAGGAG TTGAGACACT TCGAGAGTGA AAATATAATG 
AGCCCAGCGT GTGTGGTTTC TCCTCATGCA ACCTACTCCA CCAGCATCAT CCCAAATGCC 
ACACTGTCTT CCTCTTCCGA GGTCATCGCT ATTCCCACTG CTCAGAGTGC GGGACAGCGG 
GAAAGTAAAA GTTCTGGCTC ATCACATGCA AGGATAAAAT CCAGAGACCA CCTCATCTCC 
AGGCATGCTG TGAAAGGTGA TCCTCAGTCT CCCGGTCGCC ACTGGAATGA GGGCCATGCC 
ACCATTCTTT CACAGGACTT AGACCCTCAT TCCCCTGGTG AACCCGCACT GTTGTCCCTC 
TGTGACTCAG CCGTCCCTCT AAATGCTCCA GCAAATAGGG AGAATGGGTC CCAAGCTATG 
CCGTATAATT GTAGAAACAA CCTGGCCTTC CCAGCCCACC CCCAAGATGT GGATGGCAAG 
AGTGAATCTA GTTATTCAGG GGGCGGAGGG CACAGCAGCT CGGAGCCCTG GGAATACAAA 
TCCTCAGGTA ATGGAAGGGC ATCCCCCCTG AAGCCGCATT TAGCAACTCC TGGCTATTCC 
ACTCCCACAA GTAACATGAG CAGCTGCAGT TTGGACCAAA CGTCCAACAA AGAGGATGCT 
GGGTCGCTGT ATTCTGAGGA CCACGATGGC TACTGTGCAT CTGTGCACAC TGACTCTGGA 
CATGGATCTG GGAATCTGTG CAATAGCAGT GATGGCTTTG GGAACCCCAG GCACAGCGTG 
ATCAATGTTT TTGTTGGAAG AGCTCAGAAA AACCAAGGGG ACCGGTCCAA TTACCAGGAT 
AAATCCCTAT CAAGAAACAT CTCTTTGAAG AAAGCAAAGA AGCCTCCCCT GCCACCCTCC 
CGGACAGACT CCCTCCGCAG GATTCCCAAG AAGAGCAGCC AGTGCAACGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCAC ACTCCAGCAC TCGCTGCAGC TGAGCCTCCC AGGCAAGAGT 
GGCAGCTCGC CCTCCCAGAG CCCCTGCAGT GACTTGGAAG AGCCCTGGCT GCCCCGCTCC 
CGGAGCCAGA GCACAGTTAG TGCTGGCAGC AGCATGACTT CCGCCACCAC CCCCAATGTC 
TACTCCCTGT GCGGGGCCAC GCCATCGCAG AGTGACACAA GCAGCGTCAA GTCAGAGTAC 
ACGGACCCCT GGGGTTATTA CATTGACTAC ACGGGCATGC AGGAAGATCC GGGGAACCCG 
GCAGGGGGCT GTTCAACCAG CAGTGGGGTG CCCACTGGGA ACGGGCCAGT CCGCCATGTC 
CAAGAAGGGT CCAGAGCCAC AATGCCCCAA GTGCCCGGTG GTTCAGTCAA ACCAAAGATC 
ATGTCACCAG AGAAGTCACA CAGAGTCATT TCTCCATCCA GTGGGTATTC CAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCTGTGCCT GTGTTTTTAA AATCAGTGTC ACCAGCAAAC 
GGGAAGGGGA AGCCCAAGCC CAAGGTACCA GAAAGGAAGT CCTCTCTGAT ATCTTCAGTA 
TCCATTTCCT CATCGTCCAC TTCTCTTTCT TCTAGTACTT CTACTGAAGG AAGTGGCACT 
ATGAAGAAGC TGGATCCAGC CGTGGGCTCT CCCCCGGCTC CTCCTCCTCC TCCTGTTCCC 
TCTCCTCCAT TCCCTTGTCC TGCAGACAGG TCTCCTTTCC TTCCTCCCCC ACCTCCTGTC 
ACAGATTGCT CCCAGGGCTC TCCTCTGCCT CACTCTCCTG TGTTCCCCCC TCCGCCGCCA 
GAAGCTCTCA TTCCTTTCTG CTCCCCACCT GATTGGTGCC TTTCTCCTCC CCGCCCTGCA 
CTGAGCCCCA TTCTTCCAGA TTCACCTGTG TCCTTGCCAT TGCCCCCACC TCTCTTACCT 
TCCTCGGAAC CCCCACCTGC CCCACCTCTT GACCCCAAAT TCATGAAAGA CACCAGGCCG 
CCTTTCACAA ATTCTGGCCA GCCAGAATCC TCCCGGGGAT CCTTGAGGCC GCCTTCTACC 
AAGGAGGAGA CCAGCAGGCC CCCCATGCCC CTGATAACCA CGGAAGCATT GCAGATGGTG 
CAGTTGAGGC CCGTGAGAAA GAACTCAGGC GCTGAGGCGG CACAGTTGTC TGAACGAACA 
GCTCAGGAAC AACGAACTCC AGTTGCTCCA CAGTACCACT TAAAGCCATC TGCTTTCCTG 
AAATCCCGAA ATAGCACAAA TGAAATGGAG AGTGAAAGCC AGCCTGCCTC TGTGACAAGC 
TCGCTTCCGA CGCCTGCCAA GAGCTCGAGT CAGGGTGACC ATGGCAGTGC GGCTGAGCGT 
GGTGGCCCTG TGAGCCGCAG CCCTGGAGCT CCAAGCGCTG GGGAGGCAGA GGCTCGGCCC 
AGCCCCAGCA CCACCCCACT CCCAGACTCT TCACCCAGCA GGAAGCCACC CCCCATTTCC 
AAGAAGCCCA AACTGTTCCT GGTGGTACCA CCTCCGCAGA AAGATTTTGC AGTGGAGCCC 
GCAGAGAACG TGAGCGAAGC CCTCCGAGCT GTGCCCAGCC CCACGACGGG AGAGGAGGGC 
TCTGTGCACA GCAGGGAGGC AAAAGAGAGT TCTGCAGCCC AAGCTGGCTC TCATGCCACG 
CACCCTGGCA CCTCGGTTCT TGAGGGAGGA GCTGCAGGAT CCATGTCTCC CAGCAGAGTG 
GAAGCCAATG TCCCCATGGT TCAGCCTGAT GTCTCACCAG CCCCCAAGCA GGAGGAGCCA 
GCCGAGAACA GTGCGGATAC TGGGGGCGAT GGGGAGAGCT GCCTATCTCA ACAGGACGGA 
GCAGCTGGGG TGCCGGAGAC CAACGCAGCC GGTTCATCCT CAGAGGCCTG TGACTTCCTC 
AAGGAAGACG GGAATGATGA GGTAATGACC CCCAGTCGAC CCAGGACCAC AGAAGACCTT 
TTTGCAGCTA TTCACAGATC CAAAAGGAAA GTCCTCGGCC GTAGAGATTC AGATGATGAC 
CACTCCCGAA ACCATTCTCC CTCCCCGCCC GTGACACCCA CCGGCGCTGC CCCAAGCCTG 
GCCTCTCCAA AGCAAGTGGG GTCGATTCAG AGAAGCATCC GAAAGAGCAG CACCAGCAGT 
GACAACTTCA AAGCTCTGCT GCTGAAAAAG GGCAGTCGTT CAGACACCAG CGCCCGCATG 
TCTGCAGCAG AGATGCTCAA GAACACAGAC CCTAGATTCC AGAGGTCGAG GTCAGAGCCT 
TCCCCAGATG CCCCCGAGAG CCCGTCAAGC TGCTCCCCAA GCAAGAACAG AAGGGCGCAG 
GAGGAGTGGG CCAAGAACGA AGGCTTGATG CCTCGGAGTC TGTCCTTTTC CGGCCCCAGG 
TACGGCCGCA GCCGAACGCC GCCTTCTGCC GCCAGCAGCA GGTACAGCAT GCGGAACCGG 
ATCCAGAGCA GCCCCATGAC CGTCATCTCG GAGGGAGAAG GGGAAGCCGT GGAGCCTGTG 
GACAGCATAG CCCGCGGGGC TCTGGGCGCT GCGGAGGGAT GTTCCCTGGA CGGACTGGCG 
AGGGAGGAGA TGGACGAGGG CGGCCTGCTC TGTGGGGAGG GGCCTGCCGC CTCCCTGCAG 
CCCCAGGCCC CCGGCCCTGT GGATGGGACA GCCAGTGCAG AGGGCAGAGA GCCCTCCCCA 
CAGTGTGGCG GTTCTCTGAG CGAGGAGAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535973.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534275.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X12, mRNA.

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CloneID	OHu70404
Clone ID Related Accession (Same CDS sequence)	XM_011535973.3 , XM_011535973.2
Accession Version	XM_011535973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535973.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAA TATATTGTCT GGAGTCTGTG ACCGATTCCT CTTTCCCGTG GGGTGCAGTT 
GCGGTTTCCA ACCTAGATGA GGAAAGCCGA TGGACAGTCC ACTACACTGC CCCGTGGCAC 
CAGCAGGAGA ATGTGTTCCT TCCCACCACA AGACCCCCCT GTGTTGAGGA CCTGCACCGC 
CAAGCCAAGC TCAACCTCAA ATCAGTACTG AGGGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534275.1
CDS	157..4980
Translation	MNVIYCLESVTDSSFPWGAVAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535973.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535973.3

ORF Insert Sequence:

ATGAATGTAA TATATTGTCT GGAGTCTGTG ACCGATTCCT CTTTCCCGTG GGGTGCAGTT 
GCGGTTTCCA ACCTAGATGA GGAAAGCCGA TGGACAGTCC ACTACACTGC CCCGTGGCAC 
CAGCAGGAGA ATGTGTTCCT TCCCACCACA AGACCCCCCT GTGTTGAGGA CCTGCACCGC 
CAAGCCAAGC TCAACCTCAA ATCAGTACTG AGGGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535973.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534275.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu70404
Clone ID Related Accession (Same CDS sequence)	XM_011535973.3 , XM_011535973.2
Accession Version	XM_011535973.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAA TATATTGTCT GGAGTCTGTG ACCGATTCCT CTTTCCCGTG GGGTGCAGTT 
GCGGTTTCCA ACCTAGATGA GGAAAGCCGA TGGACAGTCC ACTACACTGC CCCGTGGCAC 
CAGCAGGAGA ATGTGTTCCT TCCCACCACA AGACCCCCCT GTGTTGAGGA CCTGCACCGC 
CAAGCCAAGC TCAACCTCAA ATCAGTACTG AGGGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534275.1
CDS	1091..5914
Translation	MNVIYCLESVTDSSFPWGAVAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535973.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535973.2

ORF Insert Sequence:

ATGAATGTAA TATATTGTCT GGAGTCTGTG ACCGATTCCT CTTTCCCGTG GGGTGCAGTT 
GCGGTTTCCA ACCTAGATGA GGAAAGCCGA TGGACAGTCC ACTACACTGC CCCGTGGCAC 
CAGCAGGAGA ATGTGTTCCT TCCCACCACA AGACCCCCCT GTGTTGAGGA CCTGCACCGC 
CAAGCCAAGC TCAACCTCAA ATCAGTACTG AGGGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535974.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534276.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu70405
Clone ID Related Accession (Same CDS sequence)	XM_011535974.2 , XM_011535974.3
Accession Version	XM_011535974.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG TTTCCAACCT AGATGAGGAA AGCCGATGGA CAGTCCACTA CACTGCCCCG 
TGGCACCAGC AGGAGAATGT GTTCCTTCCC ACCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CCAAGCTCAA CCTCAAATCA GTACTGAGGG TGATTCCAGA AGATGAGATG 
GGAGACCAGA ATTCTGGTTG CAGTGTTGTC AGTGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534276.1
CDS	1883..6706
Translation	MAAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535974.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535974.2

ORF Insert Sequence:

ATGGCAGCGG TTTCCAACCT AGATGAGGAA AGCCGATGGA CAGTCCACTA CACTGCCCCG 
TGGCACCAGC AGGAGAATGT GTTCCTTCCC ACCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CCAAGCTCAA CCTCAAATCA GTACTGAGGG TGATTCCAGA AGATGAGATG 
GGAGACCAGA ATTCTGGTTG CAGTGTTGTC AGTGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535974.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534276.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X13, mRNA.

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CloneID	OHu70405
Clone ID Related Accession (Same CDS sequence)	XM_011535974.2 , XM_011535974.3
Accession Version	XM_011535974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG TTTCCAACCT AGATGAGGAA AGCCGATGGA CAGTCCACTA CACTGCCCCG 
TGGCACCAGC AGGAGAATGT GTTCCTTCCC ACCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CCAAGCTCAA CCTCAAATCA GTACTGAGGG TGATTCCAGA AGATGAGATG 
GGAGACCAGA ATTCTGGTTG CAGTGTTGTC AGTGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534276.1
CDS	1885..6708
Translation	MAAVSNLDEESRWTVHYTAPWHQQENVFLPTTRPPCVEDLHRQAKLNLKSVLRVIPEDEMGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535974.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535974.3

ORF Insert Sequence:

ATGGCAGCGG TTTCCAACCT AGATGAGGAA AGCCGATGGA CAGTCCACTA CACTGCCCCG 
TGGCACCAGC AGGAGAATGT GTTCCTTCCC ACCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CCAAGCTCAA CCTCAAATCA GTACTGAGGG TGATTCCAGA AGATGAGATG 
GGAGACCAGA ATTCTGGTTG CAGTGTTGTC AGTGAATGCG ATAAGTTGCG GCATGATGGC 
TACCGCAGTT CTCAGTACTA CTCTCAGGGA CCCACCTTTG CGGCCAACGC CAGCCCATTC 
TGTGATGATT ACCAAGATGA AGATGAAGAA ACAGATCAAA AGTGTTCTCT GTCTTCATCA 
GAAGAAGAAA GATTTATTTC CATCAGGAGA CCTAAAACAC CAGCCTCAAG TGACTTCTCC 
GACCTTAATA CTCAGACGAA CTGGACCAAG TCGCTTCCAC TGCCAACACC AGAAGAGAAG 
ATGCGACAGC AAGCCCAAAC AGTCCAGGCT GACGTGGTGC CTATTAACAT AACTGGGGAG 
AATTTCGATC GCCAGGCCAG CCTTCGGCGG TCTCTAATTT ACACAGACAC TCTGGTAAGA 
CGACCGAAGA AAGTCAAAAG GAGAAAGACT ATTACAGGAG TCCCTGACAA CATACAGAAG 
GAGCTAGCAT CAGGCACTGG CCAAGATGAT GCTGATGGCC ACTCAGTGTA CACCCCTGAT 
CACTACTCTA CACTAGGAAG GTTCAATAGC TGTCGGTCTG CTGGGCAGCG CTCAGAAACC 
AGGGACTCCA GCTGTCAGAC GGAGGATGTG AAGGTCGTAC CACCTTCCAT GAGGAGAATC 
AGGGCACAGA AGGGGCAAGG CATTGCTGCC CAGATGGGCC ACTTCTCAGG TTCCTCTGGC 
AACATGTCTG TGCTGAGCGA TTCTGCAGGG ATCGTATTCC CTTCCCGCCT TGACAGTGAT 
GCTGGCTTCC ATAGTCTTCC GCGTTCTGGA GCAAGGGCAA ACATTCAGTC CCTTGAGCCG 
AGGCTGGGTG CCCTCGGCCC TGCAGGAGAC ATGAATGGCA CTTTCCTCTA CCAGAGAGGT 
CACCCACAAG CAGATGAAAA CTTAGGCCAT TTAGGAGGTG CCTCAGGGAC TGGAACACTT 
TTGAGACCCA AATCCCAGGA GTTGAGACAC TTCGAGAGTG AAAATATAAT GAGCCCAGCG 
TGTGTGGTTT CTCCTCATGC AACCTACTCC ACCAGCATCA TCCCAAATGC CACACTGTCT 
TCCTCTTCCG AGGTCATCGC TATTCCCACT GCTCAGAGTG CGGGACAGCG GGAAAGTAAA 
AGTTCTGGCT CATCACATGC AAGGATAAAA TCCAGAGACC ACCTCATCTC CAGGCATGCT 
GTGAAAGGTG ATCCTCAGTC TCCCGGTCGC CACTGGAATG AGGGCCATGC CACCATTCTT 
TCACAGGACT TAGACCCTCA TTCCCCTGGT GAACCCGCAC TGTTGTCCCT CTGTGACTCA 
GCCGTCCCTC TAAATGCTCC AGCAAATAGG GAGAATGGGT CCCAAGCTAT GCCGTATAAT 
TGTAGAAACA ACCTGGCCTT CCCAGCCCAC CCCCAAGATG TGGATGGCAA GAGTGAATCT 
AGTTATTCAG GGGGCGGAGG GCACAGCAGC TCGGAGCCCT GGGAATACAA ATCCTCAGGT 
AATGGAAGGG CATCCCCCCT GAAGCCGCAT TTAGCAACTC CTGGCTATTC CACTCCCACA 
AGTAACATGA GCAGCTGCAG TTTGGACCAA ACGTCCAACA AAGAGGATGC TGGGTCGCTG 
TATTCTGAGG ACCACGATGG CTACTGTGCA TCTGTGCACA CTGACTCTGG ACATGGATCT 
GGGAATCTGT GCAATAGCAG TGATGGCTTT GGGAACCCCA GGCACAGCGT GATCAATGTT 
TTTGTTGGAA GAGCTCAGAA AAACCAAGGG GACCGGTCCA ATTACCAGGA TAAATCCCTA 
TCAAGAAACA TCTCTTTGAA GAAAGCAAAG AAGCCTCCCC TGCCACCCTC CCGGACAGAC 
TCCCTCCGCA GGATTCCCAA GAAGAGCAGC CAGTGCAACG GGCAGGTGCT CAACGAGAGC 
CTGATCGCCA CACTCCAGCA CTCGCTGCAG CTGAGCCTCC CAGGCAAGAG TGGCAGCTCG 
CCCTCCCAGA GCCCCTGCAG TGACTTGGAA GAGCCCTGGC TGCCCCGCTC CCGGAGCCAG 
AGCACAGTTA GTGCTGGCAG CAGCATGACT TCCGCCACCA CCCCCAATGT CTACTCCCTG 
TGCGGGGCCA CGCCATCGCA GAGTGACACA AGCAGCGTCA AGTCAGAGTA CACGGACCCC 
TGGGGTTATT ACATTGACTA CACGGGCATG CAGGAAGATC CGGGGAACCC GGCAGGGGGC 
TGTTCAACCA GCAGTGGGGT GCCCACTGGG AACGGGCCAG TCCGCCATGT CCAAGAAGGG 
TCCAGAGCCA CAATGCCCCA AGTGCCCGGT GGTTCAGTCA AACCAAAGAT CATGTCACCA 
GAGAAGTCAC ACAGAGTCAT TTCTCCATCC AGTGGGTATT CCAGCCAGTC GAATACACCC 
ACAGCACTCA CCCCTGTGCC TGTGTTTTTA AAATCAGTGT CACCAGCAAA CGGGAAGGGG 
AAGCCCAAGC CCAAGGTACC AGAAAGGAAG TCCTCTCTGA TATCTTCAGT ATCCATTTCC 
TCATCGTCCA CTTCTCTTTC TTCTAGTACT TCTACTGAAG GAAGTGGCAC TATGAAGAAG 
CTGGATCCAG CCGTGGGCTC TCCCCCGGCT CCTCCTCCTC CTCCTGTTCC CTCTCCTCCA 
TTCCCTTGTC CTGCAGACAG GTCTCCTTTC CTTCCTCCCC CACCTCCTGT CACAGATTGC 
TCCCAGGGCT CTCCTCTGCC TCACTCTCCT GTGTTCCCCC CTCCGCCGCC AGAAGCTCTC 
ATTCCTTTCT GCTCCCCACC TGATTGGTGC CTTTCTCCTC CCCGCCCTGC ACTGAGCCCC 
ATTCTTCCAG ATTCACCTGT GTCCTTGCCA TTGCCCCCAC CTCTCTTACC TTCCTCGGAA 
CCCCCACCTG CCCCACCTCT TGACCCCAAA TTCATGAAAG ACACCAGGCC GCCTTTCACA 
AATTCTGGCC AGCCAGAATC CTCCCGGGGA TCCTTGAGGC CGCCTTCTAC CAAGGAGGAG 
ACCAGCAGGC CCCCCATGCC CCTGATAACC ACGGAAGCAT TGCAGATGGT GCAGTTGAGG 
CCCGTGAGAA AGAACTCAGG CGCTGAGGCG GCACAGTTGT CTGAACGAAC AGCTCAGGAA 
CAACGAACTC CAGTTGCTCC ACAGTACCAC TTAAAGCCAT CTGCTTTCCT GAAATCCCGA 
AATAGCACAA ATGAAATGGA GAGTGAAAGC CAGCCTGCCT CTGTGACAAG CTCGCTTCCG 
ACGCCTGCCA AGAGCTCGAG TCAGGGTGAC CATGGCAGTG CGGCTGAGCG TGGTGGCCCT 
GTGAGCCGCA GCCCTGGAGC TCCAAGCGCT GGGGAGGCAG AGGCTCGGCC CAGCCCCAGC 
ACCACCCCAC TCCCAGACTC TTCACCCAGC AGGAAGCCAC CCCCCATTTC CAAGAAGCCC 
AAACTGTTCC TGGTGGTACC ACCTCCGCAG AAAGATTTTG CAGTGGAGCC CGCAGAGAAC 
GTGAGCGAAG CCCTCCGAGC TGTGCCCAGC CCCACGACGG GAGAGGAGGG CTCTGTGCAC 
AGCAGGGAGG CAAAAGAGAG TTCTGCAGCC CAAGCTGGCT CTCATGCCAC GCACCCTGGC 
ACCTCGGTTC TTGAGGGAGG AGCTGCAGGA TCCATGTCTC CCAGCAGAGT GGAAGCCAAT 
GTCCCCATGG TTCAGCCTGA TGTCTCACCA GCCCCCAAGC AGGAGGAGCC AGCCGAGAAC 
AGTGCGGATA CTGGGGGCGA TGGGGAGAGC TGCCTATCTC AACAGGACGG AGCAGCTGGG 
GTGCCGGAGA CCAACGCAGC CGGTTCATCC TCAGAGGCCT GTGACTTCCT CAAGGAAGAC 
GGGAATGATG AGGTAATGAC CCCCAGTCGA CCCAGGACCA CAGAAGACCT TTTTGCAGCT 
ATTCACAGAT CCAAAAGGAA AGTCCTCGGC CGTAGAGATT CAGATGATGA CCACTCCCGA 
AACCATTCTC CCTCCCCGCC CGTGACACCC ACCGGCGCTG CCCCAAGCCT GGCCTCTCCA 
AAGCAAGTGG GGTCGATTCA GAGAAGCATC CGAAAGAGCA GCACCAGCAG TGACAACTTC 
AAAGCTCTGC TGCTGAAAAA GGGCAGTCGT TCAGACACCA GCGCCCGCAT GTCTGCAGCA 
GAGATGCTCA AGAACACAGA CCCTAGATTC CAGAGGTCGA GGTCAGAGCC TTCCCCAGAT 
GCCCCCGAGA GCCCGTCAAG CTGCTCCCCA AGCAAGAACA GAAGGGCGCA GGAGGAGTGG 
GCCAAGAACG AAGGCTTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCCAG GTACGGCCGC 
AGCCGAACGC CGCCTTCTGC CGCCAGCAGC AGGTACAGCA TGCGGAACCG GATCCAGAGC 
AGCCCCATGA CCGTCATCTC GGAGGGAGAA GGGGAAGCCG TGGAGCCTGT GGACAGCATA 
GCCCGCGGGG CTCTGGGCGC TGCGGAGGGA TGTTCCCTGG ACGGACTGGC GAGGGAGGAG 
ATGGACGAGG GCGGCCTGCT CTGTGGGGAG GGGCCTGCCG CCTCCCTGCA GCCCCAGGCC 
CCCGGCCCTG TGGATGGGAC AGCCAGTGCA GAGGGCAGAG AGCCCTCCCC ACAGTGTGGC 
GGTTCTCTGA GCGAGGAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSL1 ( XM_011535976.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534278.1 Homo sapiens NHS like 1 (NHSL1), transcript variant X15, mRNA.

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CloneID	OHu70407
Clone ID Related Accession (Same CDS sequence)	XM_011535976.1
Accession Version	XM_011535976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NHS-like protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACC AGAATTCTGG TTGCAGTGTT GTCAGTGAAT GCGATAAGTT GCGGCATGAT 
GGCTACCGCA GTTCTCAGTA CTACTCTCAG GGACCCACCT TTGCGGCCAA CGCCAGCCCA 
TTCTGTGATG ATTACCAAGA TGAAGATGAA GAAACAGATC AAAAGTGTTC TCTGTCTTCA 
TCAGAAGAAG AAAGATTTAT TTCCATCAGG AGACCTAAAA CACCAGCCTC AAGTGACTTC 
TCCGACCTTA ATACTCAGAC GAACTGGACC AAGTCGCTTC CACTGCCAAC ACCAGAAGAG 
AAGATGCGAC AGCAAGCCCA AACAGTCCAG GCTGACGTGG TGCCTATTAA CATAACTGGG 
GAGAATTTCG ATCGCCAGGC CAGCCTTCGG CGGTCTCTAA TTTACACAGA CACTCTGGTA 
AGACGACCGA AGAAAGTCAA AAGGAGAAAG ACTATTACAG GAGTCCCTGA CAACATACAG 
AAGGAGCTAG CATCAGGCAC TGGCCAAGAT GATGCTGATG GCCACTCAGT GTACACCCCT 
GATCACTACT CTACACTAGG AAGGTTCAAT AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA 
ACCAGGGACT CCAGCTGTCA GACGGAGGAT GTGAAGGTCG TACCACCTTC CATGAGGAGA 
ATCAGGGCAC AGAAGGGGCA AGGCATTGCT GCCCAGATGG GCCACTTCTC AGGTTCCTCT 
GGCAACATGT CTGTGCTGAG CGATTCTGCA GGGATCGTAT TCCCTTCCCG CCTTGACAGT 
GATGCTGGCT TCCATAGTCT TCCGCGTTCT GGAGCAAGGG CAAACATTCA GTCCCTTGAG 
CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA GACATGAATG GCACTTTCCT CTACCAGAGA 
GGTCACCCAC AAGCAGATGA AAACTTAGGC CATTTAGGAG GTGCCTCAGG GACTGGAACA 
CTTTTGAGAC CCAAATCCCA GGAGTTGAGA CACTTCGAGA GTGAAAATAT AATGAGCCCA 
GCGTGTGTGG TTTCTCCTCA TGCAACCTAC TCCACCAGCA TCATCCCAAA TGCCACACTG 
TCTTCCTCTT CCGAGGTCAT CGCTATTCCC ACTGCTCAGA GTGCGGGACA GCGGGAAAGT 
AAAAGTTCTG GCTCATCACA TGCAAGGATA AAATCCAGAG ACCACCTCAT CTCCAGGCAT 
GCTGTGAAAG GTGATCCTCA GTCTCCCGGT CGCCACTGGA ATGAGGGCCA TGCCACCATT 
CTTTCACAGG ACTTAGACCC TCATTCCCCT GGTGAACCCG CACTGTTGTC CCTCTGTGAC 
TCAGCCGTCC CTCTAAATGC TCCAGCAAAT AGGGAGAATG GGTCCCAAGC TATGCCGTAT 
AATTGTAGAA ACAACCTGGC CTTCCCAGCC CACCCCCAAG ATGTGGATGG CAAGAGTGAA 
TCTAGTTATT CAGGGGGCGG AGGGCACAGC AGCTCGGAGC CCTGGGAATA CAAATCCTCA 
GGTAATGGAA GGGCATCCCC CCTGAAGCCG CATTTAGCAA CTCCTGGCTA TTCCACTCCC 
ACAAGTAACA TGAGCAGCTG CAGTTTGGAC CAAACGTCCA ACAAAGAGGA TGCTGGGTCG 
CTGTATTCTG AGGACCACGA TGGCTACTGT GCATCTGTGC ACACTGACTC TGGACATGGA 
TCTGGGAATC TGTGCAATAG CAGTGATGGC TTTGGGAACC CCAGGCACAG CGTGATCAAT 
GTTTTTGTTG GAAGAGCTCA GAAAAACCAA GGGGACCGGT CCAATTACCA GGATAAATCC 
CTATCAAGAA ACATCTCTTT GAAGAAAGCA AAGAAGCCTC CCCTGCCACC CTCCCGGACA 
GACTCCCTCC GCAGGATTCC CAAGAAGAGC AGCCAGTGCA ACGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCACACTCCA GCACTCGCTG CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC 
TCGCCCTCCC AGAGCCCCTG CAGTGACTTG GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC 
CAGAGCACAG TTAGTGCTGG CAGCAGCATG ACTTCCGCCA CCACCCCCAA TGTCTACTCC 
CTGTGCGGGG CCACGCCATC GCAGAGTGAC ACAAGCAGCG TCAAGTCAGA GTACACGGAC 
CCCTGGGGTT ATTACATTGA CTACACGGGC ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG 
GGCTGTTCAA CCAGCAGTGG GGTGCCCACT GGGAACGGGC CAGTCCGCCA TGTCCAAGAA 
GGGTCCAGAG CCACAATGCC CCAAGTGCCC GGTGGTTCAG TCAAACCAAA GATCATGTCA 
CCAGAGAAGT CACACAGAGT CATTTCTCCA TCCAGTGGGT ATTCCAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCTGT GCCTGTGTTT TTAAAATCAG TGTCACCAGC AAACGGGAAG 
GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG AAGTCCTCTC TGATATCTTC AGTATCCATT 
TCCTCATCGT CCACTTCTCT TTCTTCTAGT ACTTCTACTG AAGGAAGTGG CACTATGAAG 
AAGCTGGATC CAGCCGTGGG CTCTCCCCCG GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT 
CCATTCCCTT GTCCTGCAGA CAGGTCTCCT TTCCTTCCTC CCCCACCTCC TGTCACAGAT 
TGCTCCCAGG GCTCTCCTCT GCCTCACTCT CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT 
CTCATTCCTT TCTGCTCCCC ACCTGATTGG TGCCTTTCTC CTCCCCGCCC TGCACTGAGC 
CCCATTCTTC CAGATTCACC TGTGTCCTTG CCATTGCCCC CACCTCTCTT ACCTTCCTCG 
GAACCCCCAC CTGCCCCACC TCTTGACCCC AAATTCATGA AAGACACCAG GCCGCCTTTC 
ACAAATTCTG GCCAGCCAGA ATCCTCCCGG GGATCCTTGA GGCCGCCTTC TACCAAGGAG 
GAGACCAGCA GGCCCCCCAT GCCCCTGATA ACCACGGAAG CATTGCAGAT GGTGCAGTTG 
AGGCCCGTGA GAAAGAACTC AGGCGCTGAG GCGGCACAGT TGTCTGAACG AACAGCTCAG 
GAACAACGAA CTCCAGTTGC TCCACAGTAC CACTTAAAGC CATCTGCTTT CCTGAAATCC 
CGAAATAGCA CAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT 
CCGACGCCTG CCAAGAGCTC GAGTCAGGGT GACCATGGCA GTGCGGCTGA GCGTGGTGGC 
CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC 
AGCACCACCC CACTCCCAGA CTCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG 
CCCAAACTGT TCCTGGTGGT ACCACCTCCG CAGAAAGATT TTGCAGTGGA GCCCGCAGAG 
AACGTGAGCG AAGCCCTCCG AGCTGTGCCC AGCCCCACGA CGGGAGAGGA GGGCTCTGTG 
CACAGCAGGG AGGCAAAAGA GAGTTCTGCA GCCCAAGCTG GCTCTCATGC CACGCACCCT 
GGCACCTCGG TTCTTGAGGG AGGAGCTGCA GGATCCATGT CTCCCAGCAG AGTGGAAGCC 
AATGTCCCCA TGGTTCAGCC TGATGTCTCA CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG 
AACAGTGCGG ATACTGGGGG CGATGGGGAG AGCTGCCTAT CTCAACAGGA CGGAGCAGCT 
GGGGTGCCGG AGACCAACGC AGCCGGTTCA TCCTCAGAGG CCTGTGACTT CCTCAAGGAA 
GACGGGAATG ATGAGGTAAT GACCCCCAGT CGACCCAGGA CCACAGAAGA CCTTTTTGCA 
GCTATTCACA GATCCAAAAG GAAAGTCCTC GGCCGTAGAG ATTCAGATGA TGACCACTCC 
CGAAACCATT CTCCCTCCCC GCCCGTGACA CCCACCGGCG CTGCCCCAAG CCTGGCCTCT 
CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC ATCCGAAAGA GCAGCACCAG CAGTGACAAC 
TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT CGTTCAGACA CCAGCGCCCG CATGTCTGCA 
GCAGAGATGC TCAAGAACAC AGACCCTAGA TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA 
GATGCCCCCG AGAGCCCGTC AAGCTGCTCC CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG 
TGGGCCAAGA ACGAAGGCTT GATGCCTCGG AGTCTGTCCT TTTCCGGCCC CAGGTACGGC 
CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC AGCAGGTACA GCATGCGGAA CCGGATCCAG 
AGCAGCCCCA TGACCGTCAT CTCGGAGGGA GAAGGGGAAG CCGTGGAGCC TGTGGACAGC 
ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG GGATGTTCCC TGGACGGACT GGCGAGGGAG 
GAGATGGACG AGGGCGGCCT GCTCTGTGGG GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG 
GCCCCCGGCC CTGTGGATGG GACAGCCAGT GCAGAGGGCA GAGAGCCCTC CCCACAGTGT 
GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534278.1
CDS	312..4958
Translation	MGDQNSGCSVVSECDKLRHDGYRSSQYYSQGPTFAANASPFCDDYQDEDEETDQKCSLSSSEEERFISIRRPKTPASSDFSDLNTQTNWTKSLPLPTPEEKMRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTITGVPDNIQKELASGTGQDDADGHSVYTPDHYSTLGRFNSCRSAGQRSETRDSSCQTEDVKVVPPSMRRIRAQKGQGIAAQMGHFSGSSGNMSVLSDSAGIVFPSRLDSDAGFHSLPRSGARANIQSLEPRLGALGPAGDMNGTFLYQRGHPQADENLGHLGGASGTGTLLRPKSQELRHFESENIMSPACVVSPHATYSTSIIPNATLSSSSEVIAIPTAQSAGQRESKSSGSSHARIKSRDHLISRHAVKGDPQSPGRHWNEGHATILSQDLDPHSPGEPALLSLCDSAVPLNAPANRENGSQAMPYNCRNNLAFPAHPQDVDGKSESSYSGGGGHSSSEPWEYKSSGNGRASPLKPHLATPGYSTPTSNMSSCSLDQTSNKEDAGSLYSEDHDGYCASVHTDSGHGSGNLCNSSDGFGNPRHSVINVFVGRAQKNQGDRSNYQDKSLSRNISLKKAKKPPLPPSRTDSLRRIPKKSSQCNGQVLNESLIATLQHSLQLSLPGKSGSSPSQSPCSDLEEPWLPRSRSQSTVSAGSSMTSATTPNVYSLCGATPSQSDTSSVKSEYTDPWGYYIDYTGMQEDPGNPAGGCSTSSGVPTGNGPVRHVQEGSRATMPQVPGGSVKPKIMSPEKSHRVISPSSGYSSQSNTPTALTPVPVFLKSVSPANGKGKPKPKVPERKSSLISSVSISSSSTSLSSSTSTEGSGTMKKLDPAVGSPPAPPPPPVPSPPFPCPADRSPFLPPPPPVTDCSQGSPLPHSPVFPPPPPEALIPFCSPPDWCLSPPRPALSPILPDSPVSLPLPPPLLPSSEPPPAPPLDPKFMKDTRPPFTNSGQPESSRGSLRPPSTKEETSRPPMPLITTEALQMVQLRPVRKNSGAEAAQLSERTAQEQRTPVAPQYHLKPSAFLKSRNSTNEMESESQPASVTSSLPTPAKSSSQGDHGSAAERGGPVSRSPGAPSAGEAEARPSPSTTPLPDSSPSRKPPPISKKPKLFLVVPPPQKDFAVEPAENVSEALRAVPSPTTGEEGSVHSREAKESSAAQAGSHATHPGTSVLEGGAAGSMSPSRVEANVPMVQPDVSPAPKQEEPAENSADTGGDGESCLSQQDGAAGVPETNAAGSSSEACDFLKEDGNDEVMTPSRPRTTEDLFAAIHRSKRKVLGRRDSDDDHSRNHSPSPPVTPTGAAPSLASPKQVGSIQRSIRKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKNTDPRFQRSRSEPSPDAPESPSSCSPSKNRRAQEEWAKNEGLMPRSLSFSGPRYGRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEAVEPVDSIARGALGAAEGCSLDGLAREEMDEGGLLCGEGPAASLQPQAPGPVDGTASAEGREPSPQCGGSLSEES

Target ORF information:

RefSeq Version	XM_011535976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NHS like 1 (NHSL1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535976.1

ORF Insert Sequence:

ATGGGAGACC AGAATTCTGG TTGCAGTGTT GTCAGTGAAT GCGATAAGTT GCGGCATGAT 
GGCTACCGCA GTTCTCAGTA CTACTCTCAG GGACCCACCT TTGCGGCCAA CGCCAGCCCA 
TTCTGTGATG ATTACCAAGA TGAAGATGAA GAAACAGATC AAAAGTGTTC TCTGTCTTCA 
TCAGAAGAAG AAAGATTTAT TTCCATCAGG AGACCTAAAA CACCAGCCTC AAGTGACTTC 
TCCGACCTTA ATACTCAGAC GAACTGGACC AAGTCGCTTC CACTGCCAAC ACCAGAAGAG 
AAGATGCGAC AGCAAGCCCA AACAGTCCAG GCTGACGTGG TGCCTATTAA CATAACTGGG 
GAGAATTTCG ATCGCCAGGC CAGCCTTCGG CGGTCTCTAA TTTACACAGA CACTCTGGTA 
AGACGACCGA AGAAAGTCAA AAGGAGAAAG ACTATTACAG GAGTCCCTGA CAACATACAG 
AAGGAGCTAG CATCAGGCAC TGGCCAAGAT GATGCTGATG GCCACTCAGT GTACACCCCT 
GATCACTACT CTACACTAGG AAGGTTCAAT AGCTGTCGGT CTGCTGGGCA GCGCTCAGAA 
ACCAGGGACT CCAGCTGTCA GACGGAGGAT GTGAAGGTCG TACCACCTTC CATGAGGAGA 
ATCAGGGCAC AGAAGGGGCA AGGCATTGCT GCCCAGATGG GCCACTTCTC AGGTTCCTCT 
GGCAACATGT CTGTGCTGAG CGATTCTGCA GGGATCGTAT TCCCTTCCCG CCTTGACAGT 
GATGCTGGCT TCCATAGTCT TCCGCGTTCT GGAGCAAGGG CAAACATTCA GTCCCTTGAG 
CCGAGGCTGG GTGCCCTCGG CCCTGCAGGA GACATGAATG GCACTTTCCT CTACCAGAGA 
GGTCACCCAC AAGCAGATGA AAACTTAGGC CATTTAGGAG GTGCCTCAGG GACTGGAACA 
CTTTTGAGAC CCAAATCCCA GGAGTTGAGA CACTTCGAGA GTGAAAATAT AATGAGCCCA 
GCGTGTGTGG TTTCTCCTCA TGCAACCTAC TCCACCAGCA TCATCCCAAA TGCCACACTG 
TCTTCCTCTT CCGAGGTCAT CGCTATTCCC ACTGCTCAGA GTGCGGGACA GCGGGAAAGT 
AAAAGTTCTG GCTCATCACA TGCAAGGATA AAATCCAGAG ACCACCTCAT CTCCAGGCAT 
GCTGTGAAAG GTGATCCTCA GTCTCCCGGT CGCCACTGGA ATGAGGGCCA TGCCACCATT 
CTTTCACAGG ACTTAGACCC TCATTCCCCT GGTGAACCCG CACTGTTGTC CCTCTGTGAC 
TCAGCCGTCC CTCTAAATGC TCCAGCAAAT AGGGAGAATG GGTCCCAAGC TATGCCGTAT 
AATTGTAGAA ACAACCTGGC CTTCCCAGCC CACCCCCAAG ATGTGGATGG CAAGAGTGAA 
TCTAGTTATT CAGGGGGCGG AGGGCACAGC AGCTCGGAGC CCTGGGAATA CAAATCCTCA 
GGTAATGGAA GGGCATCCCC CCTGAAGCCG CATTTAGCAA CTCCTGGCTA TTCCACTCCC 
ACAAGTAACA TGAGCAGCTG CAGTTTGGAC CAAACGTCCA ACAAAGAGGA TGCTGGGTCG 
CTGTATTCTG AGGACCACGA TGGCTACTGT GCATCTGTGC ACACTGACTC TGGACATGGA 
TCTGGGAATC TGTGCAATAG CAGTGATGGC TTTGGGAACC CCAGGCACAG CGTGATCAAT 
GTTTTTGTTG GAAGAGCTCA GAAAAACCAA GGGGACCGGT CCAATTACCA GGATAAATCC 
CTATCAAGAA ACATCTCTTT GAAGAAAGCA AAGAAGCCTC CCCTGCCACC CTCCCGGACA 
GACTCCCTCC GCAGGATTCC CAAGAAGAGC AGCCAGTGCA ACGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCACACTCCA GCACTCGCTG CAGCTGAGCC TCCCAGGCAA GAGTGGCAGC 
TCGCCCTCCC AGAGCCCCTG CAGTGACTTG GAAGAGCCCT GGCTGCCCCG CTCCCGGAGC 
CAGAGCACAG TTAGTGCTGG CAGCAGCATG ACTTCCGCCA CCACCCCCAA TGTCTACTCC 
CTGTGCGGGG CCACGCCATC GCAGAGTGAC ACAAGCAGCG TCAAGTCAGA GTACACGGAC 
CCCTGGGGTT ATTACATTGA CTACACGGGC ATGCAGGAAG ATCCGGGGAA CCCGGCAGGG 
GGCTGTTCAA CCAGCAGTGG GGTGCCCACT GGGAACGGGC CAGTCCGCCA TGTCCAAGAA 
GGGTCCAGAG CCACAATGCC CCAAGTGCCC GGTGGTTCAG TCAAACCAAA GATCATGTCA 
CCAGAGAAGT CACACAGAGT CATTTCTCCA TCCAGTGGGT ATTCCAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCTGT GCCTGTGTTT TTAAAATCAG TGTCACCAGC AAACGGGAAG 
GGGAAGCCCA AGCCCAAGGT ACCAGAAAGG AAGTCCTCTC TGATATCTTC AGTATCCATT 
TCCTCATCGT CCACTTCTCT TTCTTCTAGT ACTTCTACTG AAGGAAGTGG CACTATGAAG 
AAGCTGGATC CAGCCGTGGG CTCTCCCCCG GCTCCTCCTC CTCCTCCTGT TCCCTCTCCT 
CCATTCCCTT GTCCTGCAGA CAGGTCTCCT TTCCTTCCTC CCCCACCTCC TGTCACAGAT 
TGCTCCCAGG GCTCTCCTCT GCCTCACTCT CCTGTGTTCC CCCCTCCGCC GCCAGAAGCT 
CTCATTCCTT TCTGCTCCCC ACCTGATTGG TGCCTTTCTC CTCCCCGCCC TGCACTGAGC 
CCCATTCTTC CAGATTCACC TGTGTCCTTG CCATTGCCCC CACCTCTCTT ACCTTCCTCG 
GAACCCCCAC CTGCCCCACC TCTTGACCCC AAATTCATGA AAGACACCAG GCCGCCTTTC 
ACAAATTCTG GCCAGCCAGA ATCCTCCCGG GGATCCTTGA GGCCGCCTTC TACCAAGGAG 
GAGACCAGCA GGCCCCCCAT GCCCCTGATA ACCACGGAAG CATTGCAGAT GGTGCAGTTG 
AGGCCCGTGA GAAAGAACTC AGGCGCTGAG GCGGCACAGT TGTCTGAACG AACAGCTCAG 
GAACAACGAA CTCCAGTTGC TCCACAGTAC CACTTAAAGC CATCTGCTTT CCTGAAATCC 
CGAAATAGCA CAAATGAAAT GGAGAGTGAA AGCCAGCCTG CCTCTGTGAC AAGCTCGCTT 
CCGACGCCTG CCAAGAGCTC GAGTCAGGGT GACCATGGCA GTGCGGCTGA GCGTGGTGGC 
CCTGTGAGCC GCAGCCCTGG AGCTCCAAGC GCTGGGGAGG CAGAGGCTCG GCCCAGCCCC 
AGCACCACCC CACTCCCAGA CTCTTCACCC AGCAGGAAGC CACCCCCCAT TTCCAAGAAG 
CCCAAACTGT TCCTGGTGGT ACCACCTCCG CAGAAAGATT TTGCAGTGGA GCCCGCAGAG 
AACGTGAGCG AAGCCCTCCG AGCTGTGCCC AGCCCCACGA CGGGAGAGGA GGGCTCTGTG 
CACAGCAGGG AGGCAAAAGA GAGTTCTGCA GCCCAAGCTG GCTCTCATGC CACGCACCCT 
GGCACCTCGG TTCTTGAGGG AGGAGCTGCA GGATCCATGT CTCCCAGCAG AGTGGAAGCC 
AATGTCCCCA TGGTTCAGCC TGATGTCTCA CCAGCCCCCA AGCAGGAGGA GCCAGCCGAG 
AACAGTGCGG ATACTGGGGG CGATGGGGAG AGCTGCCTAT CTCAACAGGA CGGAGCAGCT 
GGGGTGCCGG AGACCAACGC AGCCGGTTCA TCCTCAGAGG CCTGTGACTT CCTCAAGGAA 
GACGGGAATG ATGAGGTAAT GACCCCCAGT CGACCCAGGA CCACAGAAGA CCTTTTTGCA 
GCTATTCACA GATCCAAAAG GAAAGTCCTC GGCCGTAGAG ATTCAGATGA TGACCACTCC 
CGAAACCATT CTCCCTCCCC GCCCGTGACA CCCACCGGCG CTGCCCCAAG CCTGGCCTCT 
CCAAAGCAAG TGGGGTCGAT TCAGAGAAGC ATCCGAAAGA GCAGCACCAG CAGTGACAAC 
TTCAAAGCTC TGCTGCTGAA AAAGGGCAGT CGTTCAGACA CCAGCGCCCG CATGTCTGCA 
GCAGAGATGC TCAAGAACAC AGACCCTAGA TTCCAGAGGT CGAGGTCAGA GCCTTCCCCA 
GATGCCCCCG AGAGCCCGTC AAGCTGCTCC CCAAGCAAGA ACAGAAGGGC GCAGGAGGAG 
TGGGCCAAGA ACGAAGGCTT GATGCCTCGG AGTCTGTCCT TTTCCGGCCC CAGGTACGGC 
CGCAGCCGAA CGCCGCCTTC TGCCGCCAGC AGCAGGTACA GCATGCGGAA CCGGATCCAG 
AGCAGCCCCA TGACCGTCAT CTCGGAGGGA GAAGGGGAAG CCGTGGAGCC TGTGGACAGC 
ATAGCCCGCG GGGCTCTGGG CGCTGCGGAG GGATGTTCCC TGGACGGACT GGCGAGGGAG 
GAGATGGACG AGGGCGGCCT GCTCTGTGGG GAGGGGCCTG CCGCCTCCCT GCAGCCCCAG 
GCCCCCGGCC CTGTGGATGG GACAGCCAGT GCAGAGGGCA GAGAGCCCTC CCCACAGTGT 
GGCGGTTCTC TGAGCGAGGA GAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NHSL1
Entrez Gene ID	57224
Full Name	NHS like 1
Synonyms	C6orf63
General protein information	Preferred Names NHS like 1 Names NHS-like protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q24.1

Pan troglodytes (chimpanzee)	NHSL1	XP_001171693.2
Mus musculus (house mouse)	Nhsl1	NP_775566.3
Danio rerio (zebrafish)	nhsl1b	XP_005160821.1
Canis lupus familiaris (dog)	NHSL1	XP_005615510.1
Rattus norvegicus (Norway rat)	Nhsl1	XP_006227729.1
	NHSL1	XP_001095123.2
Xenopus tropicalis (tropical clawed frog)	nhsl1	NP_001135597.1
Homo sapiens (human)	NHSL1	NP_065197.1
Bos taurus (cattle)	NHSL1	NP_001178467.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NHSL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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