PSMD8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMD8
Entrez Gene ID	5714
Full Name	proteasome 26S subunit, non-ATPase 8
Synonyms	HEL-S-91n,HIP6,HYPF,Nin1p,Rpn12,S14,p31
General protein information	Preferred Names proteasome 26S subunit, non-ATPase 8 Names 26S proteasome non-ATPase regulatory subunit 8 26S proteasome regulatory subunit RPN12 26S proteasome regulatory subunit S14 26S proteasome regulatory subunit p31 epididymis secretory sperm binding protein Li 91n proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.2
Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_002812.4	NP_002803.2	26S proteasome non-ATPase regulatory subunit 8

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSMD8	NP_002803.2
Drosophila melanogaster (fruit fly)	Rpn12	NP_648904.1
Schizosaccharomyces pombe (fission yeast)	rpn12	NP_596750.2
Neurospora crassa	NCU10067	XP_957882.1
Arabidopsis thaliana (thale cress)	RPN12a	NP_176633.1
Oryza sativa (rice)	Os07g0435100	NP_001059500.1
Pan troglodytes (chimpanzee)	PSMD8	NP_001238915.1
	LOC721897	XP_001118092.2
Canis lupus familiaris (dog)	PSMD8	XP_533681.1
Bos taurus (cattle)	PSMD8	NP_001030283.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002606	XP_312329.4
Rattus norvegicus (Norway rat)	Psmd8	NP_001094301.1
Caenorhabditis elegans (roundworm)	rpn-12	NP_496489.1
Saccharomyces cerevisiae (baker's yeast)	RPN12	NP_116710.1
Eremothecium gossypii	AGOS_AFR278W	NP_985825.1
Mus musculus (house mouse)	Psmd8	NP_080821.3
Danio rerio (zebrafish)	psmd8	NP_001002131.1
Kluyveromyces lactis	KLLA0D11374g	XP_453568.1
Magnaporthe oryzae (rice blast fungus)	MGG_03512	XP_003716407.1

Gene Ontology
Bibliography

Related articles in PubMed

Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
Kottemann MC, Conti BA, Lach FP, Smogorzewska A
Molecular cell69(1)24-35.e5(2018 Jan)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.
Wang X, Chen CF, Baker PR, Chen PL, Kaiser P, Huang L
Biochemistry46(11)3553-65(2007 Mar)

Evidence for a newly discovered cellular anti-HIV-1 phenotype.
Simon JH, Gaddis NC, Fouchier RA, Malim MH
Nature medicine4(12)1397-400(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings support the hypothesis that neurodegenerative disorders result primarily from a generic cell dysfunction caused by early misfolded species in the aggregation process.
Title: Prefibrillar amyloid aggregates could be generic toxins in higher organisms.

The following PSMD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu30718	NM_002812.4 Latest version!	Homo sapiens proteasome 26S subunit, non-ATPase 8 (PSMD8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMD8 ( NM_002812.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002803.2 Homo sapiens proteasome 26S subunit, non-ATPase 8 (PSMD8), mRNA.

Price & Availability↑

CloneID	OHu30718
Clone ID Related Accession (Same CDS sequence)	NM_002812.4
Accession Version	NM_002812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP369299.1 and BC001164.1. On Aug 31, 2007 this sequence version replaced NM_002812.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU668354.1, ERR279845.5397.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCATTA AGGGCAGGGC TCCGAGGGCG CCACCTCGAG AGCGACGGCG GGCTACCCGG 
GGCGGGCTGA GGCAGGTTGT AGCCCCGCCC CGGGCCTTGG GCTCCACCTC TCGGCCCCAC 
TTCCGCCGGG CAAGCGTTTG TAGGCGGCGC TGCCGTAAAT CAGGCGGTCT GCTTGCCGCA 
TCACGCAAGA TGGCGGCCGC GGCGGTGAAC GGGGCGGCAG GCTTCTCGAG CTCCGGGCCC 
GCGGCAACCT CGGGCGCTGT TCTGCAGGCC GCGACCGGCA TGTACGAGCA ACTCAAGGGC 
GAGTGGAACC GTAAAAGCCC CAATCTTAGC AAGTGCGGGG AAGAGCTGGG TCGACTCAAG 
CTAGTTCTTC TGGAGCTCAA CTTCTTGCCA ACCACAGGGA CCAAGCTGAC CAAACAGCAG 
CTAATTCTGG CCCGTGACAT ACTGGAGATC GGGGCCCAAT GGAGCATCCT ACGCAAGGAC 
ATCCCCTCCT TCGAGCGCTA CATGGCCCAG CTCAAATGCT ACTACTTTGA TTACAAGGAG 
CAGCTCCCCG AGTCAGCCTA TATGCACCAG CTCTTGGGCC TCAACCTCCT CTTCCTGCTG 
TCCCAGAACC GGGTGGCTGA GTTCCACACG GAGTTGGAGC GGCTGCCTGC CAAGGACATA 
CAGACCAATG TCTACATCAA GCACCCAGTG TCCCTGGAGC AATACCTGAT GGAGGGCAGC 
TACAACAAAG TGTTCCTGGC CAAGGGTAAC ATCCCCGCCG AGAGCTACAC CTTCTTCATT 
GACATCCTGC TCGACACTAT CAGGGATGAG ATCGCTGGGT GCATCGAGAA GGCCTACGAG 
AAAATCCTTT TCACTGAGGC CACCCGGATC CTCTTCTTCA ACACACCCAA AAAGATGACA 
GACTACGCCA AGAAGCGAGG GTGGGTCCTG GGCCCCAACA ACTACTACAG TTTTGCCAGC 
CAGCAGCAGA AGCCGGAAGA CACCACCATT CCCTCCACAG AACTGGCCAA ACAGGTCATC 
GAGTATGCCC GGCAGCTGGA GATGATCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002803.2
CDS	53..1105
Translation	MFIKGRAPRAPPRERRRATRGGLRQVVAPPRALGSTSRPHFRRASVCRRRCRKSGGLLAASRKMAAAAVNGAAGFSSSGPAATSGAVLQAATGMYEQLKGEWNRKSPNLSKCGEELGRLKLVLLELNFLPTTGTKLTKQQLILARDILEIGAQWSILRKDIPSFERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQTNVYIKHPVSLEQYLMEGSYNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIEKAYEKILFTEATRILFFNTPKKMTDYAKKRGWVLGPNNYYSFASQQQKPEDTTIPSTELAKQVIEYARQLEMIV

Target ORF information:

RefSeq Version	NM_002812.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 8 (PSMD8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002812.4

ORF Insert Sequence:

ATGTTCATTA AGGGCAGGGC TCCGAGGGCG CCACCTCGAG AGCGACGGCG GGCTACCCGG 
GGCGGGCTGA GGCAGGTTGT AGCCCCGCCC CGGGCCTTGG GCTCCACCTC TCGGCCCCAC 
TTCCGCCGGG CAAGCGTTTG TAGGCGGCGC TGCCGTAAAT CAGGCGGTCT GCTTGCCGCA 
TCACGCAAGA TGGCGGCCGC GGCGGTGAAC GGGGCGGCAG GCTTCTCGAG CTCCGGGCCC 
GCGGCAACCT CGGGCGCTGT TCTGCAGGCC GCGACCGGCA TGTACGAGCA ACTCAAGGGC 
GAGTGGAACC GTAAAAGCCC CAATCTTAGC AAGTGCGGGG AAGAGCTGGG TCGACTCAAG 
CTAGTTCTTC TGGAGCTCAA CTTCTTGCCA ACCACAGGGA CCAAGCTGAC CAAACAGCAG 
CTAATTCTGG CCCGTGACAT ACTGGAGATC GGGGCCCAAT GGAGCATCCT ACGCAAGGAC 
ATCCCCTCCT TCGAGCGCTA CATGGCCCAG CTCAAATGCT ACTACTTTGA TTACAAGGAG 
CAGCTCCCCG AGTCAGCCTA TATGCACCAG CTCTTGGGCC TCAACCTCCT CTTCCTGCTG 
TCCCAGAACC GGGTGGCTGA GTTCCACACG GAGTTGGAGC GGCTGCCTGC CAAGGACATA 
CAGACCAATG TCTACATCAA GCACCCAGTG TCCCTGGAGC AATACCTGAT GGAGGGCAGC 
TACAACAAAG TGTTCCTGGC CAAGGGTAAC ATCCCCGCCG AGAGCTACAC CTTCTTCATT 
GACATCCTGC TCGACACTAT CAGGGATGAG ATCGCTGGGT GCATCGAGAA GGCCTACGAG 
AAAATCCTTT TCACTGAGGC CACCCGGATC CTCTTCTTCA ACACACCCAA AAAGATGACA 
GACTACGCCA AGAAGCGAGG GTGGGTCCTG GGCCCCAACA ACTACTACAG TTTTGCCAGC 
CAGCAGCAGA AGCCGGAAGA CACCACCATT CCCTCCACAG AACTGGCCAA ACAGGTCATC 
GAGTATGCCC GGCAGCTGGA GATGATCGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.
Molecular cell69(1)24-35.e5(2018 Jan)
Kottemann MC,Conti BA,Lach FP,Smogorzewska A

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.
Biochemistry46(11)3553-65(2007 Mar)
Wang X,Chen CF,Baker PR,Chen PL,Kaiser P,Huang L

Evidence for a newly discovered cellular anti-HIV-1 phenotype.
Nature medicine4(12)1397-400(1998 Dec)
Simon JH,Gaddis NC,Fouchier RA,Malim MH

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PSMD8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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