CHMP1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHMP1B
Entrez Gene ID	57132
Full Name	charged multivesicular body protein 1B
Synonyms	C10orf2,C18-ORF2,C18orf2,CHMP1.5,Vps46-2,Vps46B,hVps46-2
General protein information	Preferred Names charged multivesicular body protein 1B Names charged multivesicular body protein 1b chromatin modifying protein 1B chromatin-modifying protein 1b vacuolar protein sorting 46-2 vacuolar protein sorting-associated protein 46-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.21
Summary	CHMP1B belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008].
Disorder MIM:	606486

mRNA and Protein(s)

mRNA	Protein	Name
NM_020412.5	NP_065145.2	charged multivesicular body protein 1b
NM_020412.4	NP_065145.2	charged multivesicular body protein 1b

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHMP1B	NP_065145.2
Mus musculus (house mouse)	Chmp1b	NP_077152.1
Pan troglodytes (chimpanzee)	CHMP1B	XP_003315850.1
Bos taurus (cattle)	CHMP1B	NP_001014904.1
	CHMP1B	XP_002800911.1
Canis lupus familiaris (dog)	CHMP1B	XP_005623147.1
Rattus norvegicus (Norway rat)	Chmp1b	NP_001103003.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure and membrane remodeling activity of ESCRT-III helical polymers.
McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A
Science (New York, N.Y.)350(6267)1548-51(2015 Dec)

New host factors important for respiratory syncytial virus (RSV) replication revealed by a novel microfluidics screen for interactors of matrix (M) protein.
Kipper S, Hamad S, Caly L, Avrahami D, Bacharach E, Jans DA, Gerber D, Bajorek M
Molecular & cellular proteomics : MCP14(3)532-43(2015 Mar)

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
Cashikar AG, Shim S, Roth R, Maldazys MR, Heuser JE, Hanson PI
eLife3(2014 May)

Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport.
Skalicky JJ, Arii J, Wenzel DM, Stubblefield WM, Katsuyama A, Uter NT, Bajorek M, Myszka DG, Sundquist WI
The Journal of biological chemistry287(52)43910-26(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The interaction between CL7MIT and CHMP1B and between CL7MIT and IST1 became stronger when IST1 or CHMP1B was additionally coexpressed, suggesting formation of ternary complex of calpain-7, IST1 and CHMP1B.
Title: Calpain-7 binds to CHMP1B at its second ?-helical region and forms a ternary complex with IST1.

Observational study of gene-disease association. (HuGE Navigator)
Title: Case-control association study of 65 candidate genes revealed a possible association of a SNP of HTR5A to be a factor susceptible to bipolar disease in Bulgarian population.

The 2.5-A structure of the C-terminal tail of CHMP1B with the MIT domain of spastin reveals a specific, high-affinity complex involving a noncanonical binding site between the first and third helices of the MIT domain.
Title: Structural basis for midbody targeting of spastin by the ESCRT-III protein CHMP1B.

UBPY MIT domain and another ubiquitin isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7.
Title: The MIT domain of UBPY constitutes a CHMP binding and endosomal localization signal required for efficient epidermal growth factor receptor degradation.

CHMP1B is bound by the VPS4A microtubule interacting and transport (MIT) domain.
Title: Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A.

The following CHMP1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHMP1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09157	NM_020412.5 Latest version!	Homo sapiens charged multivesicular body protein 1B (CHMP1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu09157	NM_020412.4	Homo sapiens charged multivesicular body protein 1B (CHMP1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHMP1B ( NM_020412.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065145.2 Homo sapiens charged multivesicular body protein 1B (CHMP1B), mRNA.

Price & Availability↑

CloneID	OHu09157
Clone ID Related Accession (Same CDS sequence)	NM_020412.5 , NM_020412.4
Accession Version	NM_020412.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 1b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX494191.1, BC065933.1 and AP005137.2. On May 17, 2019 this sequence version replaced NM_020412.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1803616.96171.1, DRR138524.315395.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000526991.3/ ENSP00000432279.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTAACA TGGAGAAACA CCTGTTCAAC CTGAAGTTCG CGGCCAAAGA ACTGAGTAGG 
AGTGCCAAAA AATGCGATAA GGAGGAAAAG GCCGAAAAGG CCAAAATTAA AAAGGCCATT 
CAGAAGGGCA ACATGGAAGT TGCGAGGATA CACGCCGAAA ATGCCATCCG CCAGAAGAAC 
CAGGCGGTGA ATTTCTTGAG AATGAGTGCG CGAGTCGATG CAGTGGCTGC CAGGGTCCAG 
ACGGCGGTGA CGATGGGCAA GGTGACCAAG TCGATGGCTG GTGTGGTTAA GTCGATGGAT 
GCGACATTGA AGACCATGAA TCTGGAGAAG ATTTCTGCTT TGATGGACAA ATTCGAGCAC 
CAGTTTGAGA CTCTGGACGT CCAGACGCAG CAAATGGAAG ACACGATGAG CAGCACGACG 
ACGCTCACCA CTCCCCAGAA CCAAGTGGAT ATGCTGCTCC AGGAAATGGC AGATGAGGCG 
GGCCTCGACC TCAACATGGA GCTGCCGCAG GGCCAGACCG GCTCCGTGGG CACGAGCGTG 
GCTTCGGCGG AGCAGGATGA ACTGTCTCAG AGACTGGCCC GCCTTCGGGA TCAAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065145.2
CDS	100..699
Translation	MSNMEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMSSTTTLTTPQNQVDMLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQDELSQRLARLRDQV

Target ORF information:

RefSeq Version	NM_020412.5
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 1B (CHMP1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020412.5

ORF Insert Sequence:

ATGTCTAACA TGGAGAAACA CCTGTTCAAC CTGAAGTTCG CGGCCAAAGA ACTGAGTAGG 
AGTGCCAAAA AATGCGATAA GGAGGAAAAG GCCGAAAAGG CCAAAATTAA AAAGGCCATT 
CAGAAGGGCA ACATGGAAGT TGCGAGGATA CACGCCGAAA ATGCCATCCG CCAGAAGAAC 
CAGGCGGTGA ATTTCTTGAG AATGAGTGCG CGAGTCGATG CAGTGGCTGC CAGGGTCCAG 
ACGGCGGTGA CGATGGGCAA GGTGACCAAG TCGATGGCTG GTGTGGTTAA GTCGATGGAT 
GCGACATTGA AGACCATGAA TCTGGAGAAG ATTTCTGCTT TGATGGACAA ATTCGAGCAC 
CAGTTTGAGA CTCTGGACGT CCAGACGCAG CAAATGGAAG ACACGATGAG CAGCACGACG 
ACGCTCACCA CTCCCCAGAA CCAAGTGGAT ATGCTGCTCC AGGAAATGGC AGATGAGGCG 
GGCCTCGACC TCAACATGGA GCTGCCGCAG GGCCAGACCG GCTCCGTGGG CACGAGCGTG 
GCTTCGGCGG AGCAGGATGA ACTGTCTCAG AGACTGGCCC GCCTTCGGGA TCAAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHMP1B ( NM_020412.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065145.2 Homo sapiens charged multivesicular body protein 1B (CHMP1B), mRNA.

Price & Availability↑

CloneID	OHu09157
Clone ID Related Accession (Same CDS sequence)	NM_020412.5 , NM_020412.4
Accession Version	NM_020412.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	charged multivesicular body protein 1b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX494191.1, BC065933.1 and AP005137.2. On Jul 11, 2008 this sequence version replaced NM_020412.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGTCTAACA TGGAGAAACA CCTGTTCAAC CTGAAGTTCG CGGCCAAAGA ACTGAGTAGG 
AGTGCCAAAA AATGCGATAA GGAGGAAAAG GCCGAAAAGG CCAAAATTAA AAAGGCCATT 
CAGAAGGGCA ACATGGAAGT TGCGAGGATA CACGCCGAAA ATGCCATCCG CCAGAAGAAC 
CAGGCGGTGA ATTTCTTGAG AATGAGTGCG CGAGTCGATG CAGTGGCTGC CAGGGTCCAG 
ACGGCGGTGA CGATGGGCAA GGTGACCAAG TCGATGGCTG GTGTGGTTAA GTCGATGGAT 
GCGACATTGA AGACCATGAA TCTGGAGAAG ATTTCTGCTT TGATGGACAA ATTCGAGCAC 
CAGTTTGAGA CTCTGGACGT CCAGACGCAG CAAATGGAAG ACACGATGAG CAGCACGACG 
ACGCTCACCA CTCCCCAGAA CCAAGTGGAT ATGCTGCTCC AGGAAATGGC AGATGAGGCG 
GGCCTCGACC TCAACATGGA GCTGCCGCAG GGCCAGACCG GCTCCGTGGG CACGAGCGTG 
GCTTCGGCGG AGCAGGATGA ACTGTCTCAG AGACTGGCCC GCCTTCGGGA TCAAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065145.2
CDS	123..722
Translation	MSNMEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMSSTTTLTTPQNQVDMLLQEMADEAGLDLNMELPQGQTGSVGTSVASAEQDELSQRLARLRDQV

Target ORF information:

RefSeq Version	NM_020412.4
Organism	Homo sapiens(human)
Definition	Homo sapiens charged multivesicular body protein 1B (CHMP1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020412.4

ORF Insert Sequence:

ATGTCTAACA TGGAGAAACA CCTGTTCAAC CTGAAGTTCG CGGCCAAAGA ACTGAGTAGG 
AGTGCCAAAA AATGCGATAA GGAGGAAAAG GCCGAAAAGG CCAAAATTAA AAAGGCCATT 
CAGAAGGGCA ACATGGAAGT TGCGAGGATA CACGCCGAAA ATGCCATCCG CCAGAAGAAC 
CAGGCGGTGA ATTTCTTGAG AATGAGTGCG CGAGTCGATG CAGTGGCTGC CAGGGTCCAG 
ACGGCGGTGA CGATGGGCAA GGTGACCAAG TCGATGGCTG GTGTGGTTAA GTCGATGGAT 
GCGACATTGA AGACCATGAA TCTGGAGAAG ATTTCTGCTT TGATGGACAA ATTCGAGCAC 
CAGTTTGAGA CTCTGGACGT CCAGACGCAG CAAATGGAAG ACACGATGAG CAGCACGACG 
ACGCTCACCA CTCCCCAGAA CCAAGTGGAT ATGCTGCTCC AGGAAATGGC AGATGAGGCG 
GGCCTCGACC TCAACATGGA GCTGCCGCAG GGCCAGACCG GCTCCGTGGG CACGAGCGTG 
GCTTCGGCGG AGCAGGATGA ACTGTCTCAG AGACTGGCCC GCCTTCGGGA TCAAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure and membrane remodeling activity of ESCRT-III helical polymers.
Science (New York, N.Y.)350(6267)1548-51(2015 Dec)
McCullough J,Clippinger AK,Talledge N,Skowyra ML,Saunders MG,Naismith TV,Colf LA,Afonine P,Arthur C,Sundquist WI,Hanson PI,Frost A

New host factors important for respiratory syncytial virus (RSV) replication revealed by a novel microfluidics screen for interactors of matrix (M) protein.
Molecular & cellular proteomics : MCP14(3)532-43(2015 Mar)
Kipper S,Hamad S,Caly L,Avrahami D,Bacharach E,Jans DA,Gerber D,Bajorek M

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.
eLife3(2014 May)
Cashikar AG,Shim S,Roth R,Maldazys MR,Heuser JE,Hanson PI

Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport.
The Journal of biological chemistry287(52)43910-26(2012 Dec)
Skalicky JJ,Arii J,Wenzel DM,Stubblefield WM,Katsuyama A,Uter NT,Bajorek M,Myszka DG,Sundquist WI

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CHMP1B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報