TIGAR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIGAR
Entrez Gene ID	57103
Full Name	TP53 induced glycolysis regulatory phosphatase
Synonyms	C12orf5,FR2BP
General protein information	Preferred Names TP53 induced glycolysis regulatory phosphatase Names fructose-2,6-bisphosphatase TIGAR TP53-induced glycolysis and apoptosis regulator fructose-2,6-bisphosphate 2-phosphatase probable fructose-2,6-bisphosphatase TIGAR transactivated by NS3TP2 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.32
Summary	This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008].
Disorder MIM:	610775

mRNA and Protein(s)

mRNA	Protein	Name
NM_020375.3	NP_065108.1	fructose-2,6-bisphosphatase TIGAR
NM_020375.2	NP_065108.1	fructose-2,6-bisphosphatase TIGAR

Pathways from BioSystems

Homologies

	LOC721886	XP_001118082.1
Rattus norvegicus (Norway rat)	RGD1308005	XP_006240250.1
Canis lupus familiaris (dog)	C27H12orf5	XP_543863.3
Gallus gallus (chicken)	C1H12ORF5	XP_417232.1
Xenopus tropicalis (tropical clawed frog)	c12orf5	NP_001120569.1
Homo sapiens (human)	C12orf5	NP_065108.1
Bos taurus (cattle)	C5H12orf5	NP_001069838.1
Mus musculus (house mouse)	9630033F20Rik	NP_795977.1
Pan troglodytes (chimpanzee)	C12H12orf5	XP_522324.2

Gene Ontology
Bibliography

Related articles in PubMed

miR-101 Enhances Cisplatin-Induced DNA Damage Through Decreasing Nicotinamide Adenine Dinucleotide Phosphate Levels by Directly Repressing Tp53-Induced Glycolysis and Apoptosis Regulator Expression in Prostate Cancer Cells.
Huang S, Yang Z, Ma Y, Yang Y, Wang S
DNA and cell biology36(4)303-310(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study showed that miR-101 inhibited viability, induced apoptosis, pushed glucose metabolism flux from the pentose phosphate pathway into glycolysis in prostate cancer PC3 cell line by decreasing NADPH levels by throughly directly binding to 3'-UTR of TIGAR mRNA and repressing TIGAR expression.
Title: miR-101 Enhances Cisplatin-Induced DNA Damage Through Decreasing Nicotinamide Adenine Dinucleotide Phosphate Levels by Directly Repressing Tp53-Induced Glycolysis and Apoptosis Regulator Expression in Prostate Cancer Cells.

the upregulation of hsamiR101 in ccRCC was induced by hypoxia. Its expression deceased the protein expression of TIGAR and promoted glycolysis. This regulatory pathway may represent a novel mechanism of carcinogenesis and requires further investigation.
Title: Hypoxia-induced hsa-miR-101 promotes glycolysis by targeting TIGAR mRNA in clear cell renal cell carcinoma.

TIGAR expression in breast carcinoma cells promotes metabolic compartmentalization and tumor growth with a mitochondrial metabolic phenotype with lactate and glutamine catabolism.
Title: TP53-inducible Glycolysis and Apoptosis Regulator (TIGAR) Metabolically Reprograms Carcinoma and Stromal Cells in Breast Cancer.

High TIGAR expression was an independent predictor of poor survival and high incidence of relapse in adult patients with CN-AML. TIGAR also showed high expression in multiple human leukemia cell lines and knockdown of TIGAR activated glycolysis through PFKFB3 upregulation in human leukemia cells.
Title: TIGAR cooperated with glycolysis to inhibit the apoptosis of leukemia cells and associated with poor prognosis in patients with cytogenetically normal acute myeloid leukemia.

simultaneous mutations at all four acetylation sites completely abolish its ability to regulate metabolic targets, such as TIGAR and SLC7A11. Moreover, p53(4KR) is still capable of inducing the p53-Mdm2 feedback loop, but p53-dependent ferroptotic responses are markedly abrogated
Title: Acetylation Is Crucial for p53-Mediated Ferroptosis and Tumor Suppression.

The following TIGAR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIGAR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19289	NM_020375.3 Latest version!	Homo sapiens TP53 induced glycolysis regulatory phosphatase (TIGAR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu19289	NM_020375.2	Homo sapiens TP53 induced glycolysis regulatory phosphatase (TIGAR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIGAR ( NM_020375.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065108.1 Homo sapiens TP53 induced glycolysis regulatory phosphatase (TIGAR), mRNA.

Price & Availability↑

CloneID	OHu19289
Clone ID Related Accession (Same CDS sequence)	NM_020375.3 , NM_020375.2
Accession Version	NM_020375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	fructose-2,6-bisphosphatase TIGAR
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ272206.1, AC008012.8 and BX098281.1. On Nov 22, 2018 this sequence version replaced NM_020375.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP201817.1, SRR1803614.162694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000179259.6/ ENSP00000179259.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCGCT TCGCTCTGAC TGTTGTCCGG CATGGAGAAA CAAGATTTAA CAAGGAGAAA 
ATAATCCAAG GACAAGGAGT AGATGAACCT CTTTCAGAAA CTGGATTTAA ACAAGCAGCA 
GCTGCTGGTA TATTTCTGAA TAATGTGAAG TTTACTCATG CTTTCTCCAG TGATCTCATG 
AGGACAAAGC AGACCATGCA TGGAATTTTG GAGAGAAGCA AATTTTGCAA AGATATGACG 
GTAAAGTATG ACTCAAGACT TCGGGAAAGG AAATACGGGG TTGTAGAAGG CAAAGCGCTA 
AGTGAGCTGA GGGCCATGGC CAAAGCAGCC AGGGAAGAGT GCCCTGTGTT TACACCGCCC 
GGAGGAGAGA CGCTGGACCA GGTGAAAATG CGTGGAATAG ACTTTTTTGA ATTTCTTTGT 
CAACTAATCC TGAAAGAAGC GGATCAAAAA GAACAGTTTT CCCAAGGATC TCCAAGCAAC 
TGTCTGGAAA CTTCTTTGGC AGAGATATTT CCTTTAGGAA AAAATCACAG CTCTAAAGTT 
AATTCAGACA GCGGTATTCC AGGATTAGCA GCCAGTGTCT TAGTTGTGAG TCACGGTGCT 
TACATGAGAA GTCTGTTTGA TTATTTTCTG ACTGACCTTA AGTGTTCCTT ACCAGCCACT 
CTGAGCAGAT CTGAACTTAT GTCAGTCACT CCCAATACAG GGATGAGTCT CTTTATCATA 
AACTTTGAGG AAGGAAGAGA AGTTAAACCA ACGGTTCAGT GTATTTGTAT GAACCTACAG 
GATCATCTAA ATGGACTGAC TGAAACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065108.1
CDS	60..872
Translation	MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR

Target ORF information:

RefSeq Version	NM_020375.3
Organism	Homo sapiens(human)
Definition	Homo sapiens TP53 induced glycolysis regulatory phosphatase (TIGAR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020375.3

ORF Insert Sequence:

ATGGCTCGCT TCGCTCTGAC TGTTGTCCGG CATGGAGAAA CAAGATTTAA CAAGGAGAAA 
ATAATCCAAG GACAAGGAGT AGATGAACCT CTTTCAGAAA CTGGATTTAA ACAAGCAGCA 
GCTGCTGGTA TATTTCTGAA TAATGTGAAG TTTACTCATG CTTTCTCCAG TGATCTCATG 
AGGACAAAGC AGACCATGCA TGGAATTTTG GAGAGAAGCA AATTTTGCAA AGATATGACG 
GTAAAGTATG ACTCAAGACT TCGGGAAAGG AAATACGGGG TTGTAGAAGG CAAAGCGCTA 
AGTGAGCTGA GGGCCATGGC CAAAGCAGCC AGGGAAGAGT GCCCTGTGTT TACACCGCCC 
GGAGGAGAGA CGCTGGACCA GGTGAAAATG CGTGGAATAG ACTTTTTTGA ATTTCTTTGT 
CAACTAATCC TGAAAGAAGC GGATCAAAAA GAACAGTTTT CCCAAGGATC TCCAAGCAAC 
TGTCTGGAAA CTTCTTTGGC AGAGATATTT CCTTTAGGAA AAAATCACAG CTCTAAAGTT 
AATTCAGACA GCGGTATTCC AGGATTAGCA GCCAGTGTCT TAGTTGTGAG TCACGGTGCT 
TACATGAGAA GTCTGTTTGA TTATTTTCTG ACTGACCTTA AGTGTTCCTT ACCAGCCACT 
CTGAGCAGAT CTGAACTTAT GTCAGTCACT CCCAATACAG GGATGAGTCT CTTTATCATA 
AACTTTGAGG AAGGAAGAGA AGTTAAACCA ACGGTTCAGT GTATTTGTAT GAACCTACAG 
GATCATCTAA ATGGACTGAC TGAAACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIGAR ( NM_020375.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065108.1 Homo sapiens TP53 induced glycolysis regulatory phosphatase (TIGAR), mRNA.

Price & Availability↑

CloneID	OHu19289
Clone ID Related Accession (Same CDS sequence)	NM_020375.3 , NM_020375.2
Accession Version	NM_020375.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	fructose-2,6-bisphosphatase TIGAR
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB480618.1, AJ272206.1, AC008012.8 and BX098281.1. On Jul 28, 2006 this sequence version replaced NM_020375.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.162694.1, SRR1660803.187222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCT TCGCTCTGAC TGTTGTCCGG CATGGAGAAA CAAGATTTAA CAAGGAGAAA 
ATAATCCAAG GACAAGGAGT AGATGAACCT CTTTCAGAAA CTGGATTTAA ACAAGCAGCA 
GCTGCTGGTA TATTTCTGAA TAATGTGAAG TTTACTCATG CTTTCTCCAG TGATCTCATG 
AGGACAAAGC AGACCATGCA TGGAATTTTG GAGAGAAGCA AATTTTGCAA AGATATGACG 
GTAAAGTATG ACTCAAGACT TCGGGAAAGG AAATACGGGG TTGTAGAAGG CAAAGCGCTA 
AGTGAGCTGA GGGCCATGGC CAAAGCAGCC AGGGAAGAGT GCCCTGTGTT TACACCGCCC 
GGAGGAGAGA CGCTGGACCA GGTGAAAATG CGTGGAATAG ACTTTTTTGA ATTTCTTTGT 
CAACTAATCC TGAAAGAAGC GGATCAAAAA GAACAGTTTT CCCAAGGATC TCCAAGCAAC 
TGTCTGGAAA CTTCTTTGGC AGAGATATTT CCTTTAGGAA AAAATCACAG CTCTAAAGTT 
AATTCAGACA GCGGTATTCC AGGATTAGCA GCCAGTGTCT TAGTTGTGAG TCACGGTGCT 
TACATGAGAA GTCTGTTTGA TTATTTTCTG ACTGACCTTA AGTGTTCCTT ACCAGCCACT 
CTGAGCAGAT CTGAACTTAT GTCAGTCACT CCCAATACAG GGATGAGTCT CTTTATCATA 
AACTTTGAGG AAGGAAGAGA AGTTAAACCA ACGGTTCAGT GTATTTGTAT GAACCTACAG 
GATCATCTAA ATGGACTGAC TGAAACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065108.1
CDS	80..892
Translation	MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR

Target ORF information:

RefSeq Version	NM_020375.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TP53 induced glycolysis regulatory phosphatase (TIGAR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020375.2

ORF Insert Sequence:

ATGGCTCGCT TCGCTCTGAC TGTTGTCCGG CATGGAGAAA CAAGATTTAA CAAGGAGAAA 
ATAATCCAAG GACAAGGAGT AGATGAACCT CTTTCAGAAA CTGGATTTAA ACAAGCAGCA 
GCTGCTGGTA TATTTCTGAA TAATGTGAAG TTTACTCATG CTTTCTCCAG TGATCTCATG 
AGGACAAAGC AGACCATGCA TGGAATTTTG GAGAGAAGCA AATTTTGCAA AGATATGACG 
GTAAAGTATG ACTCAAGACT TCGGGAAAGG AAATACGGGG TTGTAGAAGG CAAAGCGCTA 
AGTGAGCTGA GGGCCATGGC CAAAGCAGCC AGGGAAGAGT GCCCTGTGTT TACACCGCCC 
GGAGGAGAGA CGCTGGACCA GGTGAAAATG CGTGGAATAG ACTTTTTTGA ATTTCTTTGT 
CAACTAATCC TGAAAGAAGC GGATCAAAAA GAACAGTTTT CCCAAGGATC TCCAAGCAAC 
TGTCTGGAAA CTTCTTTGGC AGAGATATTT CCTTTAGGAA AAAATCACAG CTCTAAAGTT 
AATTCAGACA GCGGTATTCC AGGATTAGCA GCCAGTGTCT TAGTTGTGAG TCACGGTGCT 
TACATGAGAA GTCTGTTTGA TTATTTTCTG ACTGACCTTA AGTGTTCCTT ACCAGCCACT 
CTGAGCAGAT CTGAACTTAT GTCAGTCACT CCCAATACAG GGATGAGTCT CTTTATCATA 
AACTTTGAGG AAGGAAGAGA AGTTAAACCA ACGGTTCAGT GTATTTGTAT GAACCTACAG 
GATCATCTAA ATGGACTGAC TGAAACTCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-101 Enhances Cisplatin-Induced DNA Damage Through Decreasing Nicotinamide Adenine Dinucleotide Phosphate Levels by Directly Repressing Tp53-Induced Glycolysis and Apoptosis Regulator Expression in Prostate Cancer Cells.
DNA and cell biology36(4)303-310(2017 Apr)
Huang S,Yang Z,Ma Y,Yang Y,Wang S

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TIGAR cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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