PSMD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMD1
Entrez Gene ID	5707
Full Name	proteasome 26S subunit, non-ATPase 1
Synonyms	P112,Rpn2,S1
General protein information	Preferred Names proteasome 26S subunit, non-ATPase 1 Names 26S proteasome non-ATPase regulatory subunit 1 26S proteasome regulatory subunit RPN2 26S proteasome regulatory subunit S1 26S proteasome subunit p112 proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_002807.3	NP_002798.2	26S proteasome non-ATPase regulatory subunit 1 isoform 1
NM_002807.4	NP_002798.2	26S proteasome non-ATPase regulatory subunit 1 isoform 1
XM_017004517.1	XP_016860006.1	26S proteasome non-ATPase regulatory subunit 1 isoform X1
NM_001191037.1	NP_001177966.1	26S proteasome non-ATPase regulatory subunit 1 isoform 2
XM_017004517.2	XP_016860006.1	26S proteasome non-ATPase regulatory subunit 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PSMD1	NP_001233478.1
Canis lupus familiaris (dog)	PSMD1	XP_003433398.1
Rattus norvegicus (Norway rat)	Psmd1	NP_114184.1
Saccharomyces cerevisiae (baker's yeast)	RPN2	NP_012190.1
	PSMD1	XP_001113035.1
Danio rerio (zebrafish)	psmd1	NP_957478.1
Drosophila melanogaster (fruit fly)	Rpn2	NP_001263057.1
Neurospora crassa	NCU09450	XP_958036.1
Arabidopsis thaliana (thale cress)	AT2G32730	NP_180832.1
Xenopus tropicalis (tropical clawed frog)	psmd1	NP_998867.1
Homo sapiens (human)	PSMD1	NP_002798.2
Mus musculus (house mouse)	Psmd1	NP_081633.1
Kluyveromyces lactis	KLLA0C09284g	XP_452613.1
Eremothecium gossypii	AGOS_ACL033C	NP_983371.1
Schizosaccharomyces pombe (fission yeast)	rpn2	NP_596381.1
Gallus gallus (chicken)	PSMD1	NP_001012618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001745	XP_321340.5
Oryza sativa (rice)	Os08g0224700	NP_001061278.1
Bos taurus (cattle)	PSMD1	NP_001094547.1
Magnaporthe oryzae (rice blast fungus)	MGG_05991	XP_003711836.1
Arabidopsis thaliana (thale cress)	AT1G04810	NP_171973.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism.
VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
The Journal of biological chemistry292(23)9493-9504(2017 06)

SUMOylation of Psmd1 controls Adrm1 interaction with the proteasome.
Ryu H, Gygi SP, Azuma Y, Arnaoutov A, Dasso M
Cell reports7(6)1842-8(2014 Jun)

Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration.
Lee H, Choi AJ, Kang GY, Park HS, Kim HC, Lim HJ, Chung H
BMB reports47(5)292-7(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RPN13 binds ubiquitin with an affinity similar to that of other proteasome-associated ubiquitin receptors and that RPN2, ubiquitin, and the deubiquitylase UCH37 bind to RPN13 with independent energetics.
Title: Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism.

Together, our findings suggest that the interaction of Psmd1 with Adrm1 is controlled by SUMOylation in a manner that may alter proteasome composition and function.
Title: SUMOylation of Psmd1 controls Adrm1 interaction with the proteasome.

Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration compared to control subjects.
Title: Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration.

The 26S proteasome plays a key role in promoting apoptosis induced by high doses of UV irradiation.
Title: MG132 inhibition of proteasome blocks apoptosis induced by severe DNA damage.

p38 MAPK negatively regulates the proteasome activity by phosphorylating Thr-273 of Rpn2
Title: Osmotic stress inhibits proteasome by p38 MAPK-dependent phosphorylation.

The following PSMD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11520	NM_002807.4 Latest version!	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu11107	NM_001191037.1 Latest version!	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu89684	XM_017004517.2 Latest version!	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu11520	NM_002807.3	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu89684	XM_017004517.1	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMD1 ( NM_002807.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002798.2 Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11520
Clone ID Related Accession (Same CDS sequence)	NM_002807.3 , NM_002807.4
Accession Version	NM_002807.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA385817.1, BC094720.1, AC009407.8 and AA169719.1. On Jul 9, 2010 this sequence version replaced NM_002807.2. Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.107058.1, SRR1660803.13938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAGTGCC CAAGTGTTGT CTCTTTGTTG 
TCAGAGAGTT ACAACCCTCA TGTGCGCTAC GGAGCTGCAA TGGCCTTGGG GATATGCTGT 
GCTGGTACAG GAAACAAGGA AGCCATTAAT TTGCTAGAAC CAATGACAAA CGACCCCGTG 
AACTACGTGA GGCAAGGGGC ACTCATAGCT TCAGCTCTCA TCATGATCCA GCAGACTGAA 
ATCACTTGTC CAAAGGTGAA TCAGTTCAGA CAGCTGTATT CCAAAGTCAT CAATGATAAG 
CATGATGATG TCATGGCCAA GTTTGGCGCT ATTCTGGCCC AGGGCATACT GGATGCAGGT 
GGTCATAATG TCACAATCTC CTTGCAGTCC AGGACTGGGC ATACTCATAT GCCTTCTGTG 
GTTGGCGTCC TTGTATTTAC CCAGTTTTGG TTCTGGTTTC CTCTTTCACA CTTCCTGTCA 
TTGGCTTATA CCCCTACCTG TGTCATTGGC CTTAACAAGG ACTTAAAGAT GCCGAAAGTT 
CAGTATAAAT CGAACTGTAA ACCATCCACA TTTGCATATC CTGCCCCTCT GGAAGTACCA 
AAAGAAAAAG AAAAGGAAAA GGTTTCTACT GCTGTATTAT CTATAACTGC CAAGGCTAAA 
AAGAAGGAAA AAGAAAAGGA AAAAAAGGAG GAGGAGAAAA TGGAAGTGGA TGAGGCAGAG 
AAAAAGGAGG AAAAAGAGAA GAAAAAAGAA CCTGAGCCAA ACTTCCAGTT ATTGGATAAC 
CCAGCCCGAG TTATGCCTGC CCAGCTTAAG GTCCTAACCA TGCCGGAGAC CTGTAGATAC 
CAGCCTTTCA AACCACTCTC TATTGGAGGC ATCATCATTC TGAAGGATAC CAGTGAAGAC 
ATTGAGGAGC TGGTGGAACC TGTGGCAGCA CATGGCCCAA AAATCGAGGA GGAGGAACAA 
GAGCCAGAAC CCCCAGAACC ATTTGAGTAT ATTGATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002798.2
CDS	163..3024
Translation	MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD

Target ORF information:

RefSeq Version	NM_002807.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002807.3

ORF Insert Sequence:

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAGTGCC CAAGTGTTGT CTCTTTGTTG 
TCAGAGAGTT ACAACCCTCA TGTGCGCTAC GGAGCTGCAA TGGCCTTGGG GATATGCTGT 
GCTGGTACAG GAAACAAGGA AGCCATTAAT TTGCTAGAAC CAATGACAAA CGACCCCGTG 
AACTACGTGA GGCAAGGGGC ACTCATAGCT TCAGCTCTCA TCATGATCCA GCAGACTGAA 
ATCACTTGTC CAAAGGTGAA TCAGTTCAGA CAGCTGTATT CCAAAGTCAT CAATGATAAG 
CATGATGATG TCATGGCCAA GTTTGGCGCT ATTCTGGCCC AGGGCATACT GGATGCAGGT 
GGTCATAATG TCACAATCTC CTTGCAGTCC AGGACTGGGC ATACTCATAT GCCTTCTGTG 
GTTGGCGTCC TTGTATTTAC CCAGTTTTGG TTCTGGTTTC CTCTTTCACA CTTCCTGTCA 
TTGGCTTATA CCCCTACCTG TGTCATTGGC CTTAACAAGG ACTTAAAGAT GCCGAAAGTT 
CAGTATAAAT CGAACTGTAA ACCATCCACA TTTGCATATC CTGCCCCTCT GGAAGTACCA 
AAAGAAAAAG AAAAGGAAAA GGTTTCTACT GCTGTATTAT CTATAACTGC CAAGGCTAAA 
AAGAAGGAAA AAGAAAAGGA AAAAAAGGAG GAGGAGAAAA TGGAAGTGGA TGAGGCAGAG 
AAAAAGGAGG AAAAAGAGAA GAAAAAAGAA CCTGAGCCAA ACTTCCAGTT ATTGGATAAC 
CCAGCCCGAG TTATGCCTGC CCAGCTTAAG GTCCTAACCA TGCCGGAGAC CTGTAGATAC 
CAGCCTTTCA AACCACTCTC TATTGGAGGC ATCATCATTC TGAAGGATAC CAGTGAAGAC 
ATTGAGGAGC TGGTGGAACC TGTGGCAGCA CATGGCCCAA AAATCGAGGA GGAGGAACAA 
GAGCCAGAAC CCCCAGAACC ATTTGAGTAT ATTGATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMD1 ( NM_002807.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002798.2 Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11520
Clone ID Related Accession (Same CDS sequence)	NM_002807.3 , NM_002807.4
Accession Version	NM_002807.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA385817.1, BC094720.1, AC009407.8 and AA169719.1. On Nov 23, 2018 this sequence version replaced NM_002807.3. Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.86949.1, SRR1660803.13938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308696.11/ ENSP00000309474.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAGTGCC CAAGTGTTGT CTCTTTGTTG 
TCAGAGAGTT ACAACCCTCA TGTGCGCTAC GGAGCTGCAA TGGCCTTGGG GATATGCTGT 
GCTGGTACAG GAAACAAGGA AGCCATTAAT TTGCTAGAAC CAATGACAAA CGACCCCGTG 
AACTACGTGA GGCAAGGGGC ACTCATAGCT TCAGCTCTCA TCATGATCCA GCAGACTGAA 
ATCACTTGTC CAAAGGTGAA TCAGTTCAGA CAGCTGTATT CCAAAGTCAT CAATGATAAG 
CATGATGATG TCATGGCCAA GTTTGGCGCT ATTCTGGCCC AGGGCATACT GGATGCAGGT 
GGTCATAATG TCACAATCTC CTTGCAGTCC AGGACTGGGC ATACTCATAT GCCTTCTGTG 
GTTGGCGTCC TTGTATTTAC CCAGTTTTGG TTCTGGTTTC CTCTTTCACA CTTCCTGTCA 
TTGGCTTATA CCCCTACCTG TGTCATTGGC CTTAACAAGG ACTTAAAGAT GCCGAAAGTT 
CAGTATAAAT CGAACTGTAA ACCATCCACA TTTGCATATC CTGCCCCTCT GGAAGTACCA 
AAAGAAAAAG AAAAGGAAAA GGTTTCTACT GCTGTATTAT CTATAACTGC CAAGGCTAAA 
AAGAAGGAAA AAGAAAAGGA AAAAAAGGAG GAGGAGAAAA TGGAAGTGGA TGAGGCAGAG 
AAAAAGGAGG AAAAAGAGAA GAAAAAAGAA CCTGAGCCAA ACTTCCAGTT ATTGGATAAC 
CCAGCCCGAG TTATGCCTGC CCAGCTTAAG GTCCTAACCA TGCCGGAGAC CTGTAGATAC 
CAGCCTTTCA AACCACTCTC TATTGGAGGC ATCATCATTC TGAAGGATAC CAGTGAAGAC 
ATTGAGGAGC TGGTGGAACC TGTGGCAGCA CATGGCCCAA AAATCGAGGA GGAGGAACAA 
GAGCCAGAAC CCCCAGAACC ATTTGAGTAT ATTGATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002798.2
CDS	160..3021
Translation	MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD

Target ORF information:

RefSeq Version	NM_002807.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002807.4

ORF Insert Sequence:

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAGTGCC CAAGTGTTGT CTCTTTGTTG 
TCAGAGAGTT ACAACCCTCA TGTGCGCTAC GGAGCTGCAA TGGCCTTGGG GATATGCTGT 
GCTGGTACAG GAAACAAGGA AGCCATTAAT TTGCTAGAAC CAATGACAAA CGACCCCGTG 
AACTACGTGA GGCAAGGGGC ACTCATAGCT TCAGCTCTCA TCATGATCCA GCAGACTGAA 
ATCACTTGTC CAAAGGTGAA TCAGTTCAGA CAGCTGTATT CCAAAGTCAT CAATGATAAG 
CATGATGATG TCATGGCCAA GTTTGGCGCT ATTCTGGCCC AGGGCATACT GGATGCAGGT 
GGTCATAATG TCACAATCTC CTTGCAGTCC AGGACTGGGC ATACTCATAT GCCTTCTGTG 
GTTGGCGTCC TTGTATTTAC CCAGTTTTGG TTCTGGTTTC CTCTTTCACA CTTCCTGTCA 
TTGGCTTATA CCCCTACCTG TGTCATTGGC CTTAACAAGG ACTTAAAGAT GCCGAAAGTT 
CAGTATAAAT CGAACTGTAA ACCATCCACA TTTGCATATC CTGCCCCTCT GGAAGTACCA 
AAAGAAAAAG AAAAGGAAAA GGTTTCTACT GCTGTATTAT CTATAACTGC CAAGGCTAAA 
AAGAAGGAAA AAGAAAAGGA AAAAAAGGAG GAGGAGAAAA TGGAAGTGGA TGAGGCAGAG 
AAAAAGGAGG AAAAAGAGAA GAAAAAAGAA CCTGAGCCAA ACTTCCAGTT ATTGGATAAC 
CCAGCCCGAG TTATGCCTGC CCAGCTTAAG GTCCTAACCA TGCCGGAGAC CTGTAGATAC 
CAGCCTTTCA AACCACTCTC TATTGGAGGC ATCATCATTC TGAAGGATAC CAGTGAAGAC 
ATTGAGGAGC TGGTGGAACC TGTGGCAGCA CATGGCCCAA AAATCGAGGA GGAGGAACAA 
GAGCCAGAAC CCCCAGAACC ATTTGAGTAT ATTGATGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMD1 ( NM_001191037.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177966.1 Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 2, mRNA.

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CloneID	OHu11107
Clone ID Related Accession (Same CDS sequence)	NM_001191037.1
Accession Version	NM_001191037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA385817.1, BC064398.1, AC009407.8 and AA169719.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.42758.1, SRR1803615.158450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END##

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAGTGCC CAAGTGTTGT CTCTTTGTTG 
TCAGAGAGTT ACAACCCTCA TGTGCGCTAC GGAGCTGCAA TGGCCTTGGG GATATGCTGT 
GCTGGTACAG GAAACAAGGA AGCCATTAAT TTGCTAGAAC CAATGACAAA CGACCCCGTG 
AACTACGTGA GGCAAGGGGC ACTCATAGCT TCAGCTCTCA TCATGATCCA GCAGACTGAA 
ATCACTTGTC CAAAGGTGAA TCAGTTCAGA CAGCTGTATT CCAAAGTCAT CAATGATAAG 
CATGATGATG TCATGGCCAA GTTTGGCGCT ATTCTGGCCC AGGGCATACT GGATGCAGGT 
GGTCATAATG TCACAATCTC CTTGCAGTCC AGGACTGGGC ATACTCATAT GCCTTCTGTG 
GTTGGCGTCC TTGTATTTAC CCAGTTTTGG TTCTGGTTTC CTCTTTCACA CTTCCTGTCA 
TTGGCTTATA CCCCTACCTG TGTCATTGGC CTTAACAAGG ACTTAAAGGT TTCTACTGCT 
GTATTATCTA TAACTGCCAA GGCTAAAAAG AAGGAAAAAG AAAAGGAAAA AAAGGAGGAG 
GAGAAAATGG AAGTGGATGA GGCAGAGAAA AAGGAGGAAA AAGAGAAGAA AAAAGAACCT 
GAGCCAAACT TCCAGTTATT GGATAACCCA GCCCGAGTTA TGCCTGCCCA GCTTAAGGTC 
CTAACCATGC CGGAGACCTG TAGATACCAG CCTTTCAAAC CACTCTCTAT TGGAGGCATC 
ATCATTCTGA AGGATACCAG TGAAGACATT GAGGAGCTGG TGGAACCTGT GGCAGCACAT 
GGCCCAAAAA TCGAGGAGGA GGAACAAGAG CCAGAACCCC CAGAACCATT TGAGTATATT 
GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177966.1
CDS	163..2931
Translation	MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD

Target ORF information:

RefSeq Version	NM_001191037.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191037.1

ORF Insert Sequence:

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAGTGCC CAAGTGTTGT CTCTTTGTTG 
TCAGAGAGTT ACAACCCTCA TGTGCGCTAC GGAGCTGCAA TGGCCTTGGG GATATGCTGT 
GCTGGTACAG GAAACAAGGA AGCCATTAAT TTGCTAGAAC CAATGACAAA CGACCCCGTG 
AACTACGTGA GGCAAGGGGC ACTCATAGCT TCAGCTCTCA TCATGATCCA GCAGACTGAA 
ATCACTTGTC CAAAGGTGAA TCAGTTCAGA CAGCTGTATT CCAAAGTCAT CAATGATAAG 
CATGATGATG TCATGGCCAA GTTTGGCGCT ATTCTGGCCC AGGGCATACT GGATGCAGGT 
GGTCATAATG TCACAATCTC CTTGCAGTCC AGGACTGGGC ATACTCATAT GCCTTCTGTG 
GTTGGCGTCC TTGTATTTAC CCAGTTTTGG TTCTGGTTTC CTCTTTCACA CTTCCTGTCA 
TTGGCTTATA CCCCTACCTG TGTCATTGGC CTTAACAAGG ACTTAAAGGT TTCTACTGCT 
GTATTATCTA TAACTGCCAA GGCTAAAAAG AAGGAAAAAG AAAAGGAAAA AAAGGAGGAG 
GAGAAAATGG AAGTGGATGA GGCAGAGAAA AAGGAGGAAA AAGAGAAGAA AAAAGAACCT 
GAGCCAAACT TCCAGTTATT GGATAACCCA GCCCGAGTTA TGCCTGCCCA GCTTAAGGTC 
CTAACCATGC CGGAGACCTG TAGATACCAG CCTTTCAAAC CACTCTCTAT TGGAGGCATC 
ATCATTCTGA AGGATACCAG TGAAGACATT GAGGAGCTGG TGGAACCTGT GGCAGCACAT 
GGCCCAAAAA TCGAGGAGGA GGAACAAGAG CCAGAACCCC CAGAACCATT TGAGTATATT 
GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMD1 ( XM_017004517.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860006.1 Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant X1, mRNA.

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CloneID	OHu89684
Clone ID Related Accession (Same CDS sequence)	XM_017004517.1 , XM_017004517.2
Accession Version	XM_017004517.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAATCCC CTCGAACTTG GAAATCCACA 
GTGGGCACCA ATGACTTACT GACTGCGCAG TTTCTTCACC TTCATTTTCG AGGGTTCCCT 
CGCAATCTGT TGCAGCGTTC CTCACGCGGG GGCACCACCT GCCGAGTCTG TCCTGCAGAT 
TCTGGCCAAG CAATGGATGA AAGAAGTTCT CAGACATGGA TATCCAGTGA AAGAGCAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860006.1
CDS	163..2265
Translation	MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQSPRTWKSTVGTNDLLTAQFLHLHFRGFPRNLLQRSSRGGTTCRVCPADSGQAMDERSSQTWISSERAG

Target ORF information:

RefSeq Version	XM_017004517.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004517.1

ORF Insert Sequence:

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAATCCC CTCGAACTTG GAAATCCACA 
GTGGGCACCA ATGACTTACT GACTGCGCAG TTTCTTCACC TTCATTTTCG AGGGTTCCCT 
CGCAATCTGT TGCAGCGTTC CTCACGCGGG GGCACCACCT GCCGAGTCTG TCCTGCAGAT 
TCTGGCCAAG CAATGGATGA AAGAAGTTCT CAGACATGGA TATCCAGTGA AAGAGCAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMD1 ( XM_017004517.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860006.1 Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant X1, mRNA.

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CloneID	OHu89684
Clone ID Related Accession (Same CDS sequence)	XM_017004517.1 , XM_017004517.2
Accession Version	XM_017004517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	26S proteasome non-ATPase regulatory subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAATCCC CTCGAACTTG GAAATCCACA 
GTGGGCACCA ATGACTTACT GACTGCGCAG TTTCTTCACC TTCATTTTCG AGGGTTCCCT 
CGCAATCTGT TGCAGCGTTC CTCACGCGGG GGCACCACCT GCCGAGTCTG TCCTGCAGAT 
TCTGGCCAAG CAATGGATGA AAGAAGTTCT CAGACATGGA TATCCAGTGA AAGAGCAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860006.1
CDS	152..2254
Translation	MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQSPRTWKSTVGTNDLLTAQFLHLHFRGFPRNLLQRSSRGGTTCRVCPADSGQAMDERSSQTWISSERAG

Target ORF information:

RefSeq Version	XM_017004517.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, non-ATPase 1 (PSMD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004517.2

ORF Insert Sequence:

ATGATCACCT CGGCCGCTGG AATTATTTCT CTTCTGGATG AAGATGAACC ACAGCTTAAG 
GAATTTGCAC TACACAAATT GAATGCAGTT GTTAATGACT TCTGGGCAGA AATTTCCGAG 
TCCGTAGACA AAATAGAGGT TTTATACGAA GATGAAGGTT TCCGGAGTCG GCAGTTTGCA 
GCCTTAGTGG CATCTAAAGT ATTTTATCAC CTGGGGGCTT TTGAGGAGTC TCTGAATTAT 
GCTCTTGGAG CAGGGGACCT CTTCAATGTC AATGATAACT CTGAATATGT GGAAACTATT 
ATAGCAAAAT GCATTGATCA CTACACCAAA CAATGTGTGG AAAATGCAGA TTTGCCTGAA 
GGAGAAAAAA AACCAATTGA CCAGAGATTG GAAGGCATCG TAAATAAAAT GTTCCAGCGA 
TGTCTAGATG ATCACAAGTA TAAACAGGCT ATTGGCATTG CTCTGGAGAC ACGAAGACTG 
GACGTCTTTG AAAAGACCAT ACTGGAGTCG AATGATGTCC CAGGAATGTT AGCTTATAGC 
CTTAAGCTCT GCATGTCTTT AATGCAGAAT AAACAGTTTC GGAATAAAGT ACTAAGAGTT 
CTAGTTAAAA TCTACATGAA CTTGGAGAAA CCTGATTTCA TCAATGTTTG TCAGTGCTTA 
ATTTTCTTAG ATGATCCTCA GGCTGTGAGT GATATCTTAG AGAAACTGGT AAAGGAAGAC 
AACCTCCTGA TGGCATATCA GATTTGTTTT GATTTGTATG AAAGTGCTAG CCAGCAGTTT 
TTGTCATCTG TAATCCAGAA TCTTCGAACT GTTGGCACCC CTATTGCTTC TGTGCCTGGA 
TCCACTAATA CGGGTACTGT TCCGGGATCA GAGAAAGACA GTGACTCGAT GGAAACAGAA 
GAAAAGACAA GCAGTGCATT TGTAGGAAAG ACACCAGAAG CCAGTCCAGA GCCTAAGGAC 
CAGACTTTGA AAATGATTAA AATTTTAAGT GGTGAAATGG CTATTGAGTT ACATCTGCAG 
TTCTTAATAC GAAACAATAA TACAGACCTC ATGATTCTAA AAAACACAAA GGATGCAGTA 
CGGAATTCTG TATGTCATAC TGCAACCGTT ATAGCAAACT CTTTTATGCA CTGTGGGACA 
ACCAGTGACC AGTTTCTTAG AGATAATTTG GAATGGTTAG CCAGAGCCAC TAACTGGGCA 
AAATTTACTG CTACAGCCAG TTTGGGTGTA ATTCATAAGG GTCATGAAAA AGAAGCATTA 
CAGTTAATGG CAACATACCT TCCCAAGGAT ACTTCTCCAG GATCAGCCTA TCAGGAAGGT 
GGAGGTCTCT ATGCACTAGG TCTTATTCAT GCCAATCATG GTGGTGATAT AATTGACTAT 
CTGCTTAATC AGCTTAAGAA CGCCAGCAAT GATATCGTTA GACACGGTGG CAGTCTGGGC 
CTTGGTTTGG CAGCCATGGG AACTGCACGT CAAGATGTTT ATGATTTGCT AAAAACAAAC 
CTTTATCAGG ATGATGCAGT AACAGGGGAA GCAGCTGGCC TGGCCCTAGG TTTGGTTATG 
TTGGGCTCTA AAAATGCTCA GGCTATTGAG GACATGGTTG GTTATGCACA AGAAACTCAA 
CATGAGAAGA TTCTGCGTGG TCTTGCAGTT GGCATAGCTT TAGTAATGTA TGGGAGGATG 
GAAGAGGCTG ATGCTCTCAT TGAATCTCTC TGTCGTGACA AGGACCCAAT TCTTCGAAGG 
TCTGGAATGT ATACTGTAGC CATGGCTTAT TGTGGCTCTG GTAACAACAA AGCAATTCGA 
CGCCTGCTAC ATGTTGCTGT AAGTGATGTT AATGATGATG TCAGGAGGGC AGCAGTAGAA 
TCACTTGGGT TCATTCTATT CAGAACCCCT GAACAATCCC CTCGAACTTG GAAATCCACA 
GTGGGCACCA ATGACTTACT GACTGCGCAG TTTCTTCACC TTCATTTTCG AGGGTTCCCT 
CGCAATCTGT TGCAGCGTTC CTCACGCGGG GGCACCACCT GCCGAGTCTG TCCTGCAGAT 
TCTGGCCAAG CAATGGATGA AAGAAGTTCT CAGACATGGA TATCCAGTGA AAGAGCAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism.
The Journal of biological chemistry292(23)9493-9504(2017 06)
VanderLinden RT,Hemmis CW,Yao T,Robinson H,Hill CP

SUMOylation of Psmd1 controls Adrm1 interaction with the proteasome.
Cell reports7(6)1842-8(2014 Jun)
Ryu H,Gygi SP,Azuma Y,Arnaoutov A,Dasso M

Increased 26S proteasome non-ATPase regulatory subunit 1 in the aqueous humor of patients with age-related macular degeneration.
BMB reports47(5)292-7(2014 May)
Lee H,Choi AJ,Kang GY,Park HS,Kim HC,Lim HJ,Chung H

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PSMD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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