DDX24 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX24
Entrez Gene ID	57062
Full Name	DEAD-box helicase 24
General protein information	Preferred Names DEAD-box helicase 24 Names ATP-dependent RNA helicase DDX24 DEAD (Asp-Glu-Ala-Asp) box helicase 24 DEAD (Asp-Glu-Ala-Asp) box polypeptide 24 DEAD box protein 24 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 24 S. cerevisiae CHL1-like helicase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.12
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level. [provided by RefSeq, Jul 2008].
Disorder MIM:	606181

mRNA and Protein(s)

mRNA	Protein	Name
NM_020414.3	NP_065147.1	ATP-dependent RNA helicase DDX24
NM_020414.4	NP_065147.1	ATP-dependent RNA helicase DDX24

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ddx24	NP_954550.1
Gallus gallus (chicken)	DDX24	XP_421338.3
Caenorhabditis elegans (roundworm)	F55F8.2	NP_491652.1
Xenopus tropicalis (tropical clawed frog)	ddx24	XP_004917136.1
Homo sapiens (human)	DDX24	NP_065147.1
	DDX24	XP_001097132.1
Bos taurus (cattle)	DDX24	NP_001092515.1
Danio rerio (zebrafish)	LOC553462	XP_005169963.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010702	XP_311419.4
Canis lupus familiaris (dog)	DDX24	XP_005623819.1
Mus musculus (house mouse)	Ddx24	NP_001152974.1
Drosophila melanogaster (fruit fly)	CG9143	NP_611467.1
Pan troglodytes (chimpanzee)	DDX24	XP_001151974.1

Gene Ontology
Bibliography

Related articles in PubMed

Negative regulation of the p300-p53 interplay by DDX24.
Shi D, Dai C, Qin J, Gu W
Oncogene35(4)528-36(2016 Jan)

MDM2 mediates nonproteolytic polyubiquitylation of the DEAD-Box RNA helicase DDX24.
Yamauchi T, Nishiyama M, Moroishi T, Yumimoto K, Nakayama KI
Molecular and cellular biology34(17)3321-40(2014 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic profiling of Myc-associated proteins.
Agrawal P, Yu K, Salomon AR, Sedivy JM
Cell cycle (Georgetown, Tex.)9(24)4908-21(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DDX24 is a negative regulator of p53 transcriptional activity.DDX24 inhibits p300 mediated acetylation of p53.
Title: Negative regulation of the p300-p53 interplay by DDX24.

depletion of DDX24 in cells impaired pre-rRNA processing and resulted both in abrogation of MDM2 function and in consequent p53 stabilization
Title: MDM2 mediates nonproteolytic polyubiquitylation of the DEAD-Box RNA helicase DDX24.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

knockdown of a DEAD-box protein named DDX24 inhibits the packaging of HIV-1 RNA and thus diminishes viral infectivity.
Title: The requirement of the DEAD-box protein DDX24 for the packaging of human immunodeficiency virus type 1 RNA.

The following DDX24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03297	NM_020414.4 Latest version!	Homo sapiens DEAD-box helicase 24 (DDX24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu03297	NM_020414.3	Homo sapiens DEAD-box helicase 24 (DDX24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX24 ( NM_020414.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065147.1 Homo sapiens DEAD-box helicase 24 (DDX24), mRNA.

Price & Availability↑

CloneID	OHu03297
Clone ID Related Accession (Same CDS sequence)	NM_020414.3 , NM_020414.4
Accession Version	NM_020414.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF214731.1. On May 30, 2001 this sequence version replaced NM_020414.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF214731.1, SRR1803613.19303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTGA AGGACACAAA ATCAAGGCCA AAGCAGTCAA GCTGTGGCAA ATTTCAGACA 
AAGGGAATCA AAGTTGTGGG AAAATGGAAG GAAGTGAAGA TTGACCCAAA TATGTTTGCA 
GATGGACAGA TGGATGACTT GGTGTGCTTT GAGGAATTGA CAGATTACCA GTTGGTCTCC 
CCTGCCAAGA ATCCCTCCAG TCTCTTCTCA AAGGAAGCAC CCAAGAGAAA GGCACAAGCT 
GTTTCAGAAG AAGAGGAGGA GGAGGAGGGA AAGTCTAGCT CACCAAAGAA AAAGATCAAG 
TTGAAGAAAA GTAAAAATGT AGCAACTGAA GGAACCAGTA CCCAGAAAGA ATTTGAAGTG 
AAAGATCCTG AGCTGGAGGC CCAGGGAGAT GACATGGTTT GTGATGATCC GGAGGCTGGG 
GAGATGACAT CAGAAAACCT GGTCCAAACT GCTCCAAAAA AGAAGAAAAA TAAAGGGAAA 
AAAGGGTTGG AGCCTTCTCA GAGCACTGCT GCCAAGGTGC CCAAAAAAGC GAAGACATGG 
ATTCCTGAAG TTCATGATCA GAAAGCAGAT GTGTCAGCTT GGAAGGACCT GTTTGTTCCC 
AGGCCGGTTC TCCGAGCACT CAGCTTTCTA GGCTTCTCTG CACCCACACC AATCCAAGCC 
CTGACCTTGG CACCTGCCAT CCGTGACAAA CTGGACATCC TTGGGGCTGC TGAGACAGGA 
AGTGGGAAAA CTCTTGCCTT TGCCATCCCA ATGATTCATG CGGTGTTGCA GTGGCAGAAG 
AGGAATGCTG CCCCTCCTCC AAGTAACACC GAAGCACCAC CTGGAGAGAC CAGAACTGAG 
GCCGGAGCTG AGACTAGATC ACCAGGCAAG GCTGAAGCTG AGTCTGATGC ATTGCCTGAC 
GATACTGTAA TTGAGAGTGA AGCACTGCCC AGTGATATTG CAGCCGAGGC CAGAGCCAAG 
ACTGGAGGCA CTGTCTCAGA CCAGGCGTTG CTCTTTGGTG ACGATGATGC TGGTGAAGGG 
CCTTCTTCCC TGATCAGGGA GAAACCTGTT CCCAAACAGA ATGAGAATGA GGAGGAAAAT 
CTTGATAAAG AGCAGACTGG AAATCTAAAA CAGGAGTTGG ATGACAAAAG CGCCACCTGT 
AAGGCATATC CAAAGCGTCC TCTGCTTGGA CTGGTTCTGA CTCCCACTCG AGAGCTGGCC 
GTCCAGGTCA AACAGCACAT TGATGCTGTG GCCAGGTTTA CAGGAATTAA AACTGCTATT 
TTGGTTGGTG GAATGTCCAC GCAGAAACAG CAGAGGATGC TGAACCGTCG TCCTGAGATT 
GTGGTTGCTA CTCCAGGCCG GCTGTGGGAA TTAATTAAAG AAAAGCATTA TCATTTGAGG 
AACCTTCGGC AGCTCAGGTG CCTGGTAGTG GATGAGGCTG ACCGGATGGT TGAGAAAGGC 
CATTTTGCTG AGCTCTCACA GCTGCTAGAG ATGCTCAATG ACTCCCAATA CAACCCAAAG 
AGACAAACGC TTGTTTTTTC TGCCACACTC ACCCTGGTGC ATCAGGCTCC TGCTCGAATC 
CTTCATAAGA AGCACACCAA GAAAATGGAT AAAACAGCCA AACTTGACCT CCTTATGCAG 
AAAATTGGCA TGAGGGGCAA GCCCAAGGTC ATTGACCTCA CAAGGAATGA GGCCACGGTG 
GAGACGCTAA CAGAGACCAA GATCCATTGT GAGACTGATG AGAAAGACTT CTACTTGTAC 
TACTTCCTGA TGCAGTATCC AGGCCGCAGC TTAGTGTTTG CCAACAGTAT CTCCTGCATC 
AAACGCCTCT CTGGGCTCCT CAAAGTCCTT GATATCATGC CCTTGACCCT GCATGCCTGT 
ATGCACCAGA AGCAGAGGCT CAGAAACCTG GAGCAGTTTG CCCGTCTGGA AGACTGTGTT 
CTCTTGGCAA CAGATGTGGC AGCTCGGGGT CTGGATATTC CTAAAGTCCA GCATGTCATC 
CATTACCAGG TCCCACGTAC CTCGGAGATT TATGTCCACC GAAGTGGTCG AACTGCTCGA 
GCTACCAATG AAGGCCTCAG TCTGATGCTC ATTGGGCCTG AGGATGTGAT CAACTTTAAG 
AAGATTTACA AAACGCTCAA GAAAGATGAG GATATCCCAC TGTTCCCCGT GCAGACAAAA 
TACATGGATG TGGTCAAGGA GCGAATCCGT TTAGCTCGAC AGATTGAGAA ATCTGAGTAT 
CGGAACTTCC AGGCTTGCCT GCACAACTCT TGGATTGAGC AGGCAGCAGC TGCCCTGGAG 
ATTGAGCTGG AAGAAGACAT GTATAAGGGA GGAAAAGCTG ACCAGCAAGA AGAACGTCGG 
AGACAAAAGC AGATGAAGGT TCTGAAGAAG GAGCTGCGCC ACCTGCTGTC CCAGCCACTG 
TTTACGGAGA GCCAGAAAAC CAAGTATCCC ACTCAGTCTG GCAAGCCGCC CCTGCTTGTG 
TCTGCCCCAA GTAAGAGCGA GTCTGCTTTG AGCTGTCTCT CCAAGCAGAA GAAGAAGAAG 
ACAAAGAAGC CGAAGGAGCC ACAGCCGGAA CAGCCACAGC CAAGTACAAG TGCAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065147.1
CDS	100..2679
Translation	MKLKDTKSRPKQSSCGKFQTKGIKVVGKWKEVKIDPNMFADGQMDDLVCFEELTDYQLVSPAKNPSSLFSKEAPKRKAQAVSEEEEEEEGKSSSPKKKIKLKKSKNVATEGTSTQKEFEVKDPELEAQGDDMVCDDPEAGEMTSENLVQTAPKKKKNKGKKGLEPSQSTAAKVPKKAKTWIPEVHDQKADVSAWKDLFVPRPVLRALSFLGFSAPTPIQALTLAPAIRDKLDILGAAETGSGKTLAFAIPMIHAVLQWQKRNAAPPPSNTEAPPGETRTEAGAETRSPGKAEAESDALPDDTVIESEALPSDIAAEARAKTGGTVSDQALLFGDDDAGEGPSSLIREKPVPKQNENEEENLDKEQTGNLKQELDDKSATCKAYPKRPLLGLVLTPTRELAVQVKQHIDAVARFTGIKTAILVGGMSTQKQQRMLNRRPEIVVATPGRLWELIKEKHYHLRNLRQLRCLVVDEADRMVEKGHFAELSQLLEMLNDSQYNPKRQTLVFSATLTLVHQAPARILHKKHTKKMDKTAKLDLLMQKIGMRGKPKVIDLTRNEATVETLTETKIHCETDEKDFYLYYFLMQYPGRSLVFANSISCIKRLSGLLKVLDIMPLTLHACMHQKQRLRNLEQFARLEDCVLLATDVAARGLDIPKVQHVIHYQVPRTSEIYVHRSGRTARATNEGLSLMLIGPEDVINFKKIYKTLKKDEDIPLFPVQTKYMDVVKERIRLARQIEKSEYRNFQACLHNSWIEQAAAALEIELEEDMYKGGKADQQEERRRQKQMKVLKKELRHLLSQPLFTESQKTKYPTQSGKPPLLVSAPSKSESALSCLSKQKKKKTKKPKEPQPEQPQPSTSAN

Target ORF information:

RefSeq Version	NM_020414.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 24 (DDX24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020414.3

ORF Insert Sequence:

ATGAAGTTGA AGGACACAAA ATCAAGGCCA AAGCAGTCAA GCTGTGGCAA ATTTCAGACA 
AAGGGAATCA AAGTTGTGGG AAAATGGAAG GAAGTGAAGA TTGACCCAAA TATGTTTGCA 
GATGGACAGA TGGATGACTT GGTGTGCTTT GAGGAATTGA CAGATTACCA GTTGGTCTCC 
CCTGCCAAGA ATCCCTCCAG TCTCTTCTCA AAGGAAGCAC CCAAGAGAAA GGCACAAGCT 
GTTTCAGAAG AAGAGGAGGA GGAGGAGGGA AAGTCTAGCT CACCAAAGAA AAAGATCAAG 
TTGAAGAAAA GTAAAAATGT AGCAACTGAA GGAACCAGTA CCCAGAAAGA ATTTGAAGTG 
AAAGATCCTG AGCTGGAGGC CCAGGGAGAT GACATGGTTT GTGATGATCC GGAGGCTGGG 
GAGATGACAT CAGAAAACCT GGTCCAAACT GCTCCAAAAA AGAAGAAAAA TAAAGGGAAA 
AAAGGGTTGG AGCCTTCTCA GAGCACTGCT GCCAAGGTGC CCAAAAAAGC GAAGACATGG 
ATTCCTGAAG TTCATGATCA GAAAGCAGAT GTGTCAGCTT GGAAGGACCT GTTTGTTCCC 
AGGCCGGTTC TCCGAGCACT CAGCTTTCTA GGCTTCTCTG CACCCACACC AATCCAAGCC 
CTGACCTTGG CACCTGCCAT CCGTGACAAA CTGGACATCC TTGGGGCTGC TGAGACAGGA 
AGTGGGAAAA CTCTTGCCTT TGCCATCCCA ATGATTCATG CGGTGTTGCA GTGGCAGAAG 
AGGAATGCTG CCCCTCCTCC AAGTAACACC GAAGCACCAC CTGGAGAGAC CAGAACTGAG 
GCCGGAGCTG AGACTAGATC ACCAGGCAAG GCTGAAGCTG AGTCTGATGC ATTGCCTGAC 
GATACTGTAA TTGAGAGTGA AGCACTGCCC AGTGATATTG CAGCCGAGGC CAGAGCCAAG 
ACTGGAGGCA CTGTCTCAGA CCAGGCGTTG CTCTTTGGTG ACGATGATGC TGGTGAAGGG 
CCTTCTTCCC TGATCAGGGA GAAACCTGTT CCCAAACAGA ATGAGAATGA GGAGGAAAAT 
CTTGATAAAG AGCAGACTGG AAATCTAAAA CAGGAGTTGG ATGACAAAAG CGCCACCTGT 
AAGGCATATC CAAAGCGTCC TCTGCTTGGA CTGGTTCTGA CTCCCACTCG AGAGCTGGCC 
GTCCAGGTCA AACAGCACAT TGATGCTGTG GCCAGGTTTA CAGGAATTAA AACTGCTATT 
TTGGTTGGTG GAATGTCCAC GCAGAAACAG CAGAGGATGC TGAACCGTCG TCCTGAGATT 
GTGGTTGCTA CTCCAGGCCG GCTGTGGGAA TTAATTAAAG AAAAGCATTA TCATTTGAGG 
AACCTTCGGC AGCTCAGGTG CCTGGTAGTG GATGAGGCTG ACCGGATGGT TGAGAAAGGC 
CATTTTGCTG AGCTCTCACA GCTGCTAGAG ATGCTCAATG ACTCCCAATA CAACCCAAAG 
AGACAAACGC TTGTTTTTTC TGCCACACTC ACCCTGGTGC ATCAGGCTCC TGCTCGAATC 
CTTCATAAGA AGCACACCAA GAAAATGGAT AAAACAGCCA AACTTGACCT CCTTATGCAG 
AAAATTGGCA TGAGGGGCAA GCCCAAGGTC ATTGACCTCA CAAGGAATGA GGCCACGGTG 
GAGACGCTAA CAGAGACCAA GATCCATTGT GAGACTGATG AGAAAGACTT CTACTTGTAC 
TACTTCCTGA TGCAGTATCC AGGCCGCAGC TTAGTGTTTG CCAACAGTAT CTCCTGCATC 
AAACGCCTCT CTGGGCTCCT CAAAGTCCTT GATATCATGC CCTTGACCCT GCATGCCTGT 
ATGCACCAGA AGCAGAGGCT CAGAAACCTG GAGCAGTTTG CCCGTCTGGA AGACTGTGTT 
CTCTTGGCAA CAGATGTGGC AGCTCGGGGT CTGGATATTC CTAAAGTCCA GCATGTCATC 
CATTACCAGG TCCCACGTAC CTCGGAGATT TATGTCCACC GAAGTGGTCG AACTGCTCGA 
GCTACCAATG AAGGCCTCAG TCTGATGCTC ATTGGGCCTG AGGATGTGAT CAACTTTAAG 
AAGATTTACA AAACGCTCAA GAAAGATGAG GATATCCCAC TGTTCCCCGT GCAGACAAAA 
TACATGGATG TGGTCAAGGA GCGAATCCGT TTAGCTCGAC AGATTGAGAA ATCTGAGTAT 
CGGAACTTCC AGGCTTGCCT GCACAACTCT TGGATTGAGC AGGCAGCAGC TGCCCTGGAG 
ATTGAGCTGG AAGAAGACAT GTATAAGGGA GGAAAAGCTG ACCAGCAAGA AGAACGTCGG 
AGACAAAAGC AGATGAAGGT TCTGAAGAAG GAGCTGCGCC ACCTGCTGTC CCAGCCACTG 
TTTACGGAGA GCCAGAAAAC CAAGTATCCC ACTCAGTCTG GCAAGCCGCC CCTGCTTGTG 
TCTGCCCCAA GTAAGAGCGA GTCTGCTTTG AGCTGTCTCT CCAAGCAGAA GAAGAAGAAG 
ACAAAGAAGC CGAAGGAGCC ACAGCCGGAA CAGCCACAGC CAAGTACAAG TGCAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX24 ( NM_020414.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065147.1 Homo sapiens DEAD-box helicase 24 (DDX24), mRNA.

Price & Availability↑

CloneID	OHu03297
Clone ID Related Accession (Same CDS sequence)	NM_020414.3 , NM_020414.4
Accession Version	NM_020414.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	ATP-dependent RNA helicase DDX24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL079302.7. On Nov 26, 2018 this sequence version replaced NM_020414.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.211782.1, SRR1803614.143602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000621632.5/ ENSP00000481495.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGTTGA AGGACACAAA ATCAAGGCCA AAGCAGTCAA GCTGTGGCAA ATTTCAGACA 
AAGGGAATCA AAGTTGTGGG AAAATGGAAG GAAGTGAAGA TTGACCCAAA TATGTTTGCA 
GATGGACAGA TGGATGACTT GGTGTGCTTT GAGGAATTGA CAGATTACCA GTTGGTCTCC 
CCTGCCAAGA ATCCCTCCAG TCTCTTCTCA AAGGAAGCAC CCAAGAGAAA GGCACAAGCT 
GTTTCAGAAG AAGAGGAGGA GGAGGAGGGA AAGTCTAGCT CACCAAAGAA AAAGATCAAG 
TTGAAGAAAA GTAAAAATGT AGCAACTGAA GGAACCAGTA CCCAGAAAGA ATTTGAAGTG 
AAAGATCCTG AGCTGGAGGC CCAGGGAGAT GACATGGTTT GTGATGATCC GGAGGCTGGG 
GAGATGACAT CAGAAAACCT GGTCCAAACT GCTCCAAAAA AGAAGAAAAA TAAAGGGAAA 
AAAGGGTTGG AGCCTTCTCA GAGCACTGCT GCCAAGGTGC CCAAAAAAGC GAAGACATGG 
ATTCCTGAAG TTCATGATCA GAAAGCAGAT GTGTCAGCTT GGAAGGACCT GTTTGTTCCC 
AGGCCGGTTC TCCGAGCACT CAGCTTTCTA GGCTTCTCTG CACCCACACC AATCCAAGCC 
CTGACCTTGG CACCTGCCAT CCGTGACAAA CTGGACATCC TTGGGGCTGC TGAGACAGGA 
AGTGGGAAAA CTCTTGCCTT TGCCATCCCA ATGATTCATG CGGTGTTGCA GTGGCAGAAG 
AGGAATGCTG CCCCTCCTCC AAGTAACACC GAAGCACCAC CTGGAGAGAC CAGAACTGAG 
GCCGGAGCTG AGACTAGATC ACCAGGCAAG GCTGAAGCTG AGTCTGATGC ATTGCCTGAC 
GATACTGTAA TTGAGAGTGA AGCACTGCCC AGTGATATTG CAGCCGAGGC CAGAGCCAAG 
ACTGGAGGCA CTGTCTCAGA CCAGGCGTTG CTCTTTGGTG ACGATGATGC TGGTGAAGGG 
CCTTCTTCCC TGATCAGGGA GAAACCTGTT CCCAAACAGA ATGAGAATGA GGAGGAAAAT 
CTTGATAAAG AGCAGACTGG AAATCTAAAA CAGGAGTTGG ATGACAAAAG CGCCACCTGT 
AAGGCATATC CAAAGCGTCC TCTGCTTGGA CTGGTTCTGA CTCCCACTCG AGAGCTGGCC 
GTCCAGGTCA AACAGCACAT TGATGCTGTG GCCAGGTTTA CAGGAATTAA AACTGCTATT 
TTGGTTGGTG GAATGTCCAC GCAGAAACAG CAGAGGATGC TGAACCGTCG TCCTGAGATT 
GTGGTTGCTA CTCCAGGCCG GCTGTGGGAA TTAATTAAAG AAAAGCATTA TCATTTGAGG 
AACCTTCGGC AGCTCAGGTG CCTGGTAGTG GATGAGGCTG ACCGGATGGT TGAGAAAGGC 
CATTTTGCTG AGCTCTCACA GCTGCTAGAG ATGCTCAATG ACTCCCAATA CAACCCAAAG 
AGACAAACGC TTGTTTTTTC TGCCACACTC ACCCTGGTGC ATCAGGCTCC TGCTCGAATC 
CTTCATAAGA AGCACACCAA GAAAATGGAT AAAACAGCCA AACTTGACCT CCTTATGCAG 
AAAATTGGCA TGAGGGGCAA GCCCAAGGTC ATTGACCTCA CAAGGAATGA GGCCACGGTG 
GAGACGCTAA CAGAGACCAA GATCCATTGT GAGACTGATG AGAAAGACTT CTACTTGTAC 
TACTTCCTGA TGCAGTATCC AGGCCGCAGC TTAGTGTTTG CCAACAGTAT CTCCTGCATC 
AAACGCCTCT CTGGGCTCCT CAAAGTCCTT GATATCATGC CCTTGACCCT GCATGCCTGT 
ATGCACCAGA AGCAGAGGCT CAGAAACCTG GAGCAGTTTG CCCGTCTGGA AGACTGTGTT 
CTCTTGGCAA CAGATGTGGC AGCTCGGGGT CTGGATATTC CTAAAGTCCA GCATGTCATC 
CATTACCAGG TCCCACGTAC CTCGGAGATT TATGTCCACC GAAGTGGTCG AACTGCTCGA 
GCTACCAATG AAGGCCTCAG TCTGATGCTC ATTGGGCCTG AGGATGTGAT CAACTTTAAG 
AAGATTTACA AAACGCTCAA GAAAGATGAG GATATCCCAC TGTTCCCCGT GCAGACAAAA 
TACATGGATG TGGTCAAGGA GCGAATCCGT TTAGCTCGAC AGATTGAGAA ATCTGAGTAT 
CGGAACTTCC AGGCTTGCCT GCACAACTCT TGGATTGAGC AGGCAGCAGC TGCCCTGGAG 
ATTGAGCTGG AAGAAGACAT GTATAAGGGA GGAAAAGCTG ACCAGCAAGA AGAACGTCGG 
AGACAAAAGC AGATGAAGGT TCTGAAGAAG GAGCTGCGCC ACCTGCTGTC CCAGCCACTG 
TTTACGGAGA GCCAGAAAAC CAAGTATCCC ACTCAGTCTG GCAAGCCGCC CCTGCTTGTG 
TCTGCCCCAA GTAAGAGCGA GTCTGCTTTG AGCTGTCTCT CCAAGCAGAA GAAGAAGAAG 
ACAAAGAAGC CGAAGGAGCC ACAGCCGGAA CAGCCACAGC CAAGTACAAG TGCAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065147.1
CDS	90..2669
Translation	MKLKDTKSRPKQSSCGKFQTKGIKVVGKWKEVKIDPNMFADGQMDDLVCFEELTDYQLVSPAKNPSSLFSKEAPKRKAQAVSEEEEEEEGKSSSPKKKIKLKKSKNVATEGTSTQKEFEVKDPELEAQGDDMVCDDPEAGEMTSENLVQTAPKKKKNKGKKGLEPSQSTAAKVPKKAKTWIPEVHDQKADVSAWKDLFVPRPVLRALSFLGFSAPTPIQALTLAPAIRDKLDILGAAETGSGKTLAFAIPMIHAVLQWQKRNAAPPPSNTEAPPGETRTEAGAETRSPGKAEAESDALPDDTVIESEALPSDIAAEARAKTGGTVSDQALLFGDDDAGEGPSSLIREKPVPKQNENEEENLDKEQTGNLKQELDDKSATCKAYPKRPLLGLVLTPTRELAVQVKQHIDAVARFTGIKTAILVGGMSTQKQQRMLNRRPEIVVATPGRLWELIKEKHYHLRNLRQLRCLVVDEADRMVEKGHFAELSQLLEMLNDSQYNPKRQTLVFSATLTLVHQAPARILHKKHTKKMDKTAKLDLLMQKIGMRGKPKVIDLTRNEATVETLTETKIHCETDEKDFYLYYFLMQYPGRSLVFANSISCIKRLSGLLKVLDIMPLTLHACMHQKQRLRNLEQFARLEDCVLLATDVAARGLDIPKVQHVIHYQVPRTSEIYVHRSGRTARATNEGLSLMLIGPEDVINFKKIYKTLKKDEDIPLFPVQTKYMDVVKERIRLARQIEKSEYRNFQACLHNSWIEQAAAALEIELEEDMYKGGKADQQEERRRQKQMKVLKKELRHLLSQPLFTESQKTKYPTQSGKPPLLVSAPSKSESALSCLSKQKKKKTKKPKEPQPEQPQPSTSAN

Target ORF information:

RefSeq Version	NM_020414.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 24 (DDX24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020414.4

ORF Insert Sequence:

ATGAAGTTGA AGGACACAAA ATCAAGGCCA AAGCAGTCAA GCTGTGGCAA ATTTCAGACA 
AAGGGAATCA AAGTTGTGGG AAAATGGAAG GAAGTGAAGA TTGACCCAAA TATGTTTGCA 
GATGGACAGA TGGATGACTT GGTGTGCTTT GAGGAATTGA CAGATTACCA GTTGGTCTCC 
CCTGCCAAGA ATCCCTCCAG TCTCTTCTCA AAGGAAGCAC CCAAGAGAAA GGCACAAGCT 
GTTTCAGAAG AAGAGGAGGA GGAGGAGGGA AAGTCTAGCT CACCAAAGAA AAAGATCAAG 
TTGAAGAAAA GTAAAAATGT AGCAACTGAA GGAACCAGTA CCCAGAAAGA ATTTGAAGTG 
AAAGATCCTG AGCTGGAGGC CCAGGGAGAT GACATGGTTT GTGATGATCC GGAGGCTGGG 
GAGATGACAT CAGAAAACCT GGTCCAAACT GCTCCAAAAA AGAAGAAAAA TAAAGGGAAA 
AAAGGGTTGG AGCCTTCTCA GAGCACTGCT GCCAAGGTGC CCAAAAAAGC GAAGACATGG 
ATTCCTGAAG TTCATGATCA GAAAGCAGAT GTGTCAGCTT GGAAGGACCT GTTTGTTCCC 
AGGCCGGTTC TCCGAGCACT CAGCTTTCTA GGCTTCTCTG CACCCACACC AATCCAAGCC 
CTGACCTTGG CACCTGCCAT CCGTGACAAA CTGGACATCC TTGGGGCTGC TGAGACAGGA 
AGTGGGAAAA CTCTTGCCTT TGCCATCCCA ATGATTCATG CGGTGTTGCA GTGGCAGAAG 
AGGAATGCTG CCCCTCCTCC AAGTAACACC GAAGCACCAC CTGGAGAGAC CAGAACTGAG 
GCCGGAGCTG AGACTAGATC ACCAGGCAAG GCTGAAGCTG AGTCTGATGC ATTGCCTGAC 
GATACTGTAA TTGAGAGTGA AGCACTGCCC AGTGATATTG CAGCCGAGGC CAGAGCCAAG 
ACTGGAGGCA CTGTCTCAGA CCAGGCGTTG CTCTTTGGTG ACGATGATGC TGGTGAAGGG 
CCTTCTTCCC TGATCAGGGA GAAACCTGTT CCCAAACAGA ATGAGAATGA GGAGGAAAAT 
CTTGATAAAG AGCAGACTGG AAATCTAAAA CAGGAGTTGG ATGACAAAAG CGCCACCTGT 
AAGGCATATC CAAAGCGTCC TCTGCTTGGA CTGGTTCTGA CTCCCACTCG AGAGCTGGCC 
GTCCAGGTCA AACAGCACAT TGATGCTGTG GCCAGGTTTA CAGGAATTAA AACTGCTATT 
TTGGTTGGTG GAATGTCCAC GCAGAAACAG CAGAGGATGC TGAACCGTCG TCCTGAGATT 
GTGGTTGCTA CTCCAGGCCG GCTGTGGGAA TTAATTAAAG AAAAGCATTA TCATTTGAGG 
AACCTTCGGC AGCTCAGGTG CCTGGTAGTG GATGAGGCTG ACCGGATGGT TGAGAAAGGC 
CATTTTGCTG AGCTCTCACA GCTGCTAGAG ATGCTCAATG ACTCCCAATA CAACCCAAAG 
AGACAAACGC TTGTTTTTTC TGCCACACTC ACCCTGGTGC ATCAGGCTCC TGCTCGAATC 
CTTCATAAGA AGCACACCAA GAAAATGGAT AAAACAGCCA AACTTGACCT CCTTATGCAG 
AAAATTGGCA TGAGGGGCAA GCCCAAGGTC ATTGACCTCA CAAGGAATGA GGCCACGGTG 
GAGACGCTAA CAGAGACCAA GATCCATTGT GAGACTGATG AGAAAGACTT CTACTTGTAC 
TACTTCCTGA TGCAGTATCC AGGCCGCAGC TTAGTGTTTG CCAACAGTAT CTCCTGCATC 
AAACGCCTCT CTGGGCTCCT CAAAGTCCTT GATATCATGC CCTTGACCCT GCATGCCTGT 
ATGCACCAGA AGCAGAGGCT CAGAAACCTG GAGCAGTTTG CCCGTCTGGA AGACTGTGTT 
CTCTTGGCAA CAGATGTGGC AGCTCGGGGT CTGGATATTC CTAAAGTCCA GCATGTCATC 
CATTACCAGG TCCCACGTAC CTCGGAGATT TATGTCCACC GAAGTGGTCG AACTGCTCGA 
GCTACCAATG AAGGCCTCAG TCTGATGCTC ATTGGGCCTG AGGATGTGAT CAACTTTAAG 
AAGATTTACA AAACGCTCAA GAAAGATGAG GATATCCCAC TGTTCCCCGT GCAGACAAAA 
TACATGGATG TGGTCAAGGA GCGAATCCGT TTAGCTCGAC AGATTGAGAA ATCTGAGTAT 
CGGAACTTCC AGGCTTGCCT GCACAACTCT TGGATTGAGC AGGCAGCAGC TGCCCTGGAG 
ATTGAGCTGG AAGAAGACAT GTATAAGGGA GGAAAAGCTG ACCAGCAAGA AGAACGTCGG 
AGACAAAAGC AGATGAAGGT TCTGAAGAAG GAGCTGCGCC ACCTGCTGTC CCAGCCACTG 
TTTACGGAGA GCCAGAAAAC CAAGTATCCC ACTCAGTCTG GCAAGCCGCC CCTGCTTGTG 
TCTGCCCCAA GTAAGAGCGA GTCTGCTTTG AGCTGTCTCT CCAAGCAGAA GAAGAAGAAG 
ACAAAGAAGC CGAAGGAGCC ACAGCCGGAA CAGCCACAGC CAAGTACAAG TGCAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Negative regulation of the p300-p53 interplay by DDX24.
Oncogene35(4)528-36(2016 Jan)
Shi D,Dai C,Qin J,Gu W

MDM2 mediates nonproteolytic polyubiquitylation of the DEAD-Box RNA helicase DDX24.
Molecular and cellular biology34(17)3321-40(2014 Sep)
Yamauchi T,Nishiyama M,Moroishi T,Yumimoto K,Nakayama KI

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic profiling of Myc-associated proteins.
Cell cycle (Georgetown, Tex.)9(24)4908-21(2010 Dec)
Agrawal P,Yu K,Salomon AR,Sedivy JM

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DDX24 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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