PSMC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMC1
Entrez Gene ID	5700
Full Name	proteasome 26S subunit, ATPase 1
Synonyms	P26S4,S4,p56
General protein information	Preferred Names proteasome 26S subunit, ATPase 1 Names 26S proteasome regulatory subunit 4 26S protease regulatory subunit 4 26S proteasome AAA-ATPase subunit RPT2 proteasome (prosome, macropain) 26S subunit, ATPase, 1 proteasome 26S ATPase subunit 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.11
Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder. [provided by RefSeq, Jul 2008].
Disorder MIM:	602706

mRNA and Protein(s)

mRNA	Protein	Name
NM_002802.2	NP_002793.2	26S proteasome regulatory subunit 4 isoform a
NM_002802.3	NP_002793.2	26S proteasome regulatory subunit 4 isoform a
NM_001330212.1	NP_001317141.1	26S proteasome regulatory subunit 4 isoform b
XM_017021470.1	XP_016876959.1	26S proteasome regulatory subunit 4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PSMC1	XP_510114.2
Caenorhabditis elegans (roundworm)	rpt-2	NP_504558.1
Arabidopsis thaliana (thale cress)	AT2G20140	NP_179604.1
Arabidopsis thaliana (thale cress)	RPT2a	NP_194633.1
Oryza sativa (rice)	Os07g0691800	NP_001060719.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003216	XP_312924.4
Oryza sativa (rice)	Os03g0298400	NP_001049843.1
Danio rerio (zebrafish)	psmc1a	NP_956327.1
Saccharomyces cerevisiae (baker's yeast)	RPT2	NP_010277.1
Eremothecium gossypii	AGOS_AEL011W	NP_984850.1
Homo sapiens (human)	PSMC1	NP_002793.2
	PSMC1	XP_001086338.2
Mus musculus (house mouse)	Psmc1	NP_032973.1
Rattus norvegicus (Norway rat)	Psmc1	NP_476464.1
Danio rerio (zebrafish)	psmc1b	NP_001002091.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003215	XP_312923.4
Kluyveromyces lactis	KLLA0F14707g	XP_455741.1
Magnaporthe oryzae (rice blast fungus)	MGG_05477	XP_003710342.1
Canis lupus familiaris (dog)	PSMC1	NP_001238880.1
Bos taurus (cattle)	PSMC1	NP_001014855.1
Gallus gallus (chicken)	PSMC1	NP_990289.1
Drosophila melanogaster (fruit fly)	Pros26.4	NP_524469.2
Schizosaccharomyces pombe (fission yeast)	rpt2	NP_595480.1
Neurospora crassa	NCU11271	XP_961590.2
Xenopus tropicalis (tropical clawed frog)	psmc1	NP_001039154.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene.
Czub B, Shah AZ, Alfano G, Kruczek PM, Chakarova CF, Bhattacharya SS
PloS one11(2)e0148678(2016)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

PSMC1 Gene in Parkinson's Disease.
G?mez-Garre P, Jes?s S, Carrillo F, C?ceres-Redondo MT, Bernal-Bernal I, Carballo M, Gao L, Mir P
European neurology68(4)193-8(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

P26s4 Associates with the C-Terminal Region of TOPORS.
Title: TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene.

Our results suggest that there is no association between variations or haplotypes in the PSMC1 gene and Parkinson's disease
Title: PSMC1 Gene in Parkinson's Disease.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity
Title: Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity.

Psmc1 conditional knock-out mice demonstrate that 26S proteasomal dysfunction is sufficient to trigger neurodegenerative disease.
Title: Depletion of 26S proteasomes in mouse brain neurons causes neurodegeneration and Lewy-like inclusions resembling human pale bodies.

The following PSMC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31294	NM_002802.3 Latest version!	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu85004	NM_001330212.1 Latest version!	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu85004	XM_017021470.1 Latest version!	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu31294	NM_002802.2	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMC1 ( NM_002802.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002793.2 Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31294
Clone ID Related Accession (Same CDS sequence)	NM_002802.2 , NM_002802.3
Accession Version	NM_002802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355074.5 and AL161662.6. On Nov 26, 2018 this sequence version replaced NM_002802.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.276463.1, SRR1660807.103291.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261303.13/ ENSP00000261303.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCAAA GTCAGAGTGG TGGTCATGGT CCTGGAGGTG GCAAGAAGGA TGACAAGGAC 
AAGAAAAAGA AATATGAACC TCCTGTACCA ACTAGAGTGG GGAAAAAGAA GAAGAAAACA 
AAGGGACCAG ATGCTGCCAG CAAACTGCCA CTGGTGACAC CTCACACTCA GTGCCGGTTA 
AAATTACTGA AGTTAGAGAG AATTAAAGAC TATCTTCTCA TGGAGGAAGA ATTCATTAGA 
AATCAGGAAC AAATGAAACC ATTAGAAGAA AAGCAAGAGG AGGAAAGATC AAAAGTGGAT 
GATCTGAGGG GGACCCCGAT GTCAGTAGGA ACCTTGGAAG AGATCATTGA TGACAATCAT 
GCCATCGTGT CTACATCTGT GGGCTCAGAA CACTACGTCA GCATTCTTTC ATTTGTAGAC 
AAGGATCTGC TGGAACCTGG CTGCTCGGTC CTGCTCAACC ACAAGGTGCA TGCCGTGATA 
GGGGTGCTGA TGGATGACAC GGATCCCCTG GTCACAGTGA TGAAGGTAGA AAAGGCCCCC 
CAGGAGACCT ATGCAGATAT TGGGGGGTTG GACAACCAAA TTCAGGAAAT TAAGGAATCT 
GTGGAGCTTC CTCTCACCCA TCCTGAATAT TATGAAGAGA TGGGTATAAA GCCTCCTAAG 
GGGGTCATTC TCTATGGTCC ACCTGGCACA GGTAAAACCT TGTTAGCCAA AGCAGTAGCA 
AACCAAACCT CAGCCACTTT CTTGAGAGTG GTTGGCTCTG AACTTATTCA GAAGTACCTA 
GGTGATGGGC CCAAACTCGT ACGGGAATTG TTCCGAGTTG CTGAAGAACA TGCACCGTCC 
ATCGTGTTTA TTGATGAAAT TGACGCCATT GGGACAAAAA GATATGACTC CAATTCTGGT 
GGTGAGAGAG AAATTCAGCG AACAATGTTG GAACTGCTGA ACCAGTTGGA TGGATTTGAT 
TCTAGGGGAG ATGTGAAAGT TATCATGGCC ACAAACCGAA TAGAAACTTT GGATCCAGCA 
CTTATCAGAC CAGGCCGCAT TGACAGGAAG ATTGAGTTCC CCCTGCCTGA TGAAAAGACG 
AAGAAGCGCA TCTTTCAGAT TCACACAAGC AGGATGACGC TGGCTGATGA TGTAACCCTG 
GACGACCTGA TCATGGCTAA AGATGACCTC TCTGGTGCTG ACATCAAGGC AATCTGTACA 
GAAGCTGGTC TGATGGCCTT AAGAGAACGT AGAATGAAAG TAACAAATGA AGACTTCAAA 
AAATCTAAAG AAAATGTTCT TTATAAGAAA CAGGAAGGCA CCCCTGAGGG GCTGTATCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002793.2
CDS	46..1368
Translation	MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Target ORF information:

RefSeq Version	NM_002802.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002802.3

ORF Insert Sequence:

ATGGGTCAAA GTCAGAGTGG TGGTCATGGT CCTGGAGGTG GCAAGAAGGA TGACAAGGAC 
AAGAAAAAGA AATATGAACC TCCTGTACCA ACTAGAGTGG GGAAAAAGAA GAAGAAAACA 
AAGGGACCAG ATGCTGCCAG CAAACTGCCA CTGGTGACAC CTCACACTCA GTGCCGGTTA 
AAATTACTGA AGTTAGAGAG AATTAAAGAC TATCTTCTCA TGGAGGAAGA ATTCATTAGA 
AATCAGGAAC AAATGAAACC ATTAGAAGAA AAGCAAGAGG AGGAAAGATC AAAAGTGGAT 
GATCTGAGGG GGACCCCGAT GTCAGTAGGA ACCTTGGAAG AGATCATTGA TGACAATCAT 
GCCATCGTGT CTACATCTGT GGGCTCAGAA CACTACGTCA GCATTCTTTC ATTTGTAGAC 
AAGGATCTGC TGGAACCTGG CTGCTCGGTC CTGCTCAACC ACAAGGTGCA TGCCGTGATA 
GGGGTGCTGA TGGATGACAC GGATCCCCTG GTCACAGTGA TGAAGGTAGA AAAGGCCCCC 
CAGGAGACCT ATGCAGATAT TGGGGGGTTG GACAACCAAA TTCAGGAAAT TAAGGAATCT 
GTGGAGCTTC CTCTCACCCA TCCTGAATAT TATGAAGAGA TGGGTATAAA GCCTCCTAAG 
GGGGTCATTC TCTATGGTCC ACCTGGCACA GGTAAAACCT TGTTAGCCAA AGCAGTAGCA 
AACCAAACCT CAGCCACTTT CTTGAGAGTG GTTGGCTCTG AACTTATTCA GAAGTACCTA 
GGTGATGGGC CCAAACTCGT ACGGGAATTG TTCCGAGTTG CTGAAGAACA TGCACCGTCC 
ATCGTGTTTA TTGATGAAAT TGACGCCATT GGGACAAAAA GATATGACTC CAATTCTGGT 
GGTGAGAGAG AAATTCAGCG AACAATGTTG GAACTGCTGA ACCAGTTGGA TGGATTTGAT 
TCTAGGGGAG ATGTGAAAGT TATCATGGCC ACAAACCGAA TAGAAACTTT GGATCCAGCA 
CTTATCAGAC CAGGCCGCAT TGACAGGAAG ATTGAGTTCC CCCTGCCTGA TGAAAAGACG 
AAGAAGCGCA TCTTTCAGAT TCACACAAGC AGGATGACGC TGGCTGATGA TGTAACCCTG 
GACGACCTGA TCATGGCTAA AGATGACCTC TCTGGTGCTG ACATCAAGGC AATCTGTACA 
GAAGCTGGTC TGATGGCCTT AAGAGAACGT AGAATGAAAG TAACAAATGA AGACTTCAAA 
AAATCTAAAG AAAATGTTCT TTATAAGAAA CAGGAAGGCA CCCCTGAGGG GCTGTATCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMC1 ( NM_002802.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002793.2 Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31294
Clone ID Related Accession (Same CDS sequence)	NM_002802.2 , NM_002802.3
Accession Version	NM_002802.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L02426.1. This sequence is a reference standard in the RefSeqGene project. On Oct 30, 2002 this sequence version replaced NM_002802.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L02426.1, SRR1163657.295243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAAA GTCAGAGTGG TGGTCATGGT CCTGGAGGTG GCAAGAAGGA TGACAAGGAC 
AAGAAAAAGA AATATGAACC TCCTGTACCA ACTAGAGTGG GGAAAAAGAA GAAGAAAACA 
AAGGGACCAG ATGCTGCCAG CAAACTGCCA CTGGTGACAC CTCACACTCA GTGCCGGTTA 
AAATTACTGA AGTTAGAGAG AATTAAAGAC TATCTTCTCA TGGAGGAAGA ATTCATTAGA 
AATCAGGAAC AAATGAAACC ATTAGAAGAA AAGCAAGAGG AGGAAAGATC AAAAGTGGAT 
GATCTGAGGG GGACCCCGAT GTCAGTAGGA ACCTTGGAAG AGATCATTGA TGACAATCAT 
GCCATCGTGT CTACATCTGT GGGCTCAGAA CACTACGTCA GCATTCTTTC ATTTGTAGAC 
AAGGATCTGC TGGAACCTGG CTGCTCGGTC CTGCTCAACC ACAAGGTGCA TGCCGTGATA 
GGGGTGCTGA TGGATGACAC GGATCCCCTG GTCACAGTGA TGAAGGTAGA AAAGGCCCCC 
CAGGAGACCT ATGCAGATAT TGGGGGGTTG GACAACCAAA TTCAGGAAAT TAAGGAATCT 
GTGGAGCTTC CTCTCACCCA TCCTGAATAT TATGAAGAGA TGGGTATAAA GCCTCCTAAG 
GGGGTCATTC TCTATGGTCC ACCTGGCACA GGTAAAACCT TGTTAGCCAA AGCAGTAGCA 
AACCAAACCT CAGCCACTTT CTTGAGAGTG GTTGGCTCTG AACTTATTCA GAAGTACCTA 
GGTGATGGGC CCAAACTCGT ACGGGAATTG TTCCGAGTTG CTGAAGAACA TGCACCGTCC 
ATCGTGTTTA TTGATGAAAT TGACGCCATT GGGACAAAAA GATATGACTC CAATTCTGGT 
GGTGAGAGAG AAATTCAGCG AACAATGTTG GAACTGCTGA ACCAGTTGGA TGGATTTGAT 
TCTAGGGGAG ATGTGAAAGT TATCATGGCC ACAAACCGAA TAGAAACTTT GGATCCAGCA 
CTTATCAGAC CAGGCCGCAT TGACAGGAAG ATTGAGTTCC CCCTGCCTGA TGAAAAGACG 
AAGAAGCGCA TCTTTCAGAT TCACACAAGC AGGATGACGC TGGCTGATGA TGTAACCCTG 
GACGACCTGA TCATGGCTAA AGATGACCTC TCTGGTGCTG ACATCAAGGC AATCTGTACA 
GAAGCTGGTC TGATGGCCTT AAGAGAACGT AGAATGAAAG TAACAAATGA AGACTTCAAA 
AAATCTAAAG AAAATGTTCT TTATAAGAAA CAGGAAGGCA CCCCTGAGGG GCTGTATCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002793.2
CDS	49..1371
Translation	MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Target ORF information:

RefSeq Version	NM_002802.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002802.2

ORF Insert Sequence:

ATGGGTCAAA GTCAGAGTGG TGGTCATGGT CCTGGAGGTG GCAAGAAGGA TGACAAGGAC 
AAGAAAAAGA AATATGAACC TCCTGTACCA ACTAGAGTGG GGAAAAAGAA GAAGAAAACA 
AAGGGACCAG ATGCTGCCAG CAAACTGCCA CTGGTGACAC CTCACACTCA GTGCCGGTTA 
AAATTACTGA AGTTAGAGAG AATTAAAGAC TATCTTCTCA TGGAGGAAGA ATTCATTAGA 
AATCAGGAAC AAATGAAACC ATTAGAAGAA AAGCAAGAGG AGGAAAGATC AAAAGTGGAT 
GATCTGAGGG GGACCCCGAT GTCAGTAGGA ACCTTGGAAG AGATCATTGA TGACAATCAT 
GCCATCGTGT CTACATCTGT GGGCTCAGAA CACTACGTCA GCATTCTTTC ATTTGTAGAC 
AAGGATCTGC TGGAACCTGG CTGCTCGGTC CTGCTCAACC ACAAGGTGCA TGCCGTGATA 
GGGGTGCTGA TGGATGACAC GGATCCCCTG GTCACAGTGA TGAAGGTAGA AAAGGCCCCC 
CAGGAGACCT ATGCAGATAT TGGGGGGTTG GACAACCAAA TTCAGGAAAT TAAGGAATCT 
GTGGAGCTTC CTCTCACCCA TCCTGAATAT TATGAAGAGA TGGGTATAAA GCCTCCTAAG 
GGGGTCATTC TCTATGGTCC ACCTGGCACA GGTAAAACCT TGTTAGCCAA AGCAGTAGCA 
AACCAAACCT CAGCCACTTT CTTGAGAGTG GTTGGCTCTG AACTTATTCA GAAGTACCTA 
GGTGATGGGC CCAAACTCGT ACGGGAATTG TTCCGAGTTG CTGAAGAACA TGCACCGTCC 
ATCGTGTTTA TTGATGAAAT TGACGCCATT GGGACAAAAA GATATGACTC CAATTCTGGT 
GGTGAGAGAG AAATTCAGCG AACAATGTTG GAACTGCTGA ACCAGTTGGA TGGATTTGAT 
TCTAGGGGAG ATGTGAAAGT TATCATGGCC ACAAACCGAA TAGAAACTTT GGATCCAGCA 
CTTATCAGAC CAGGCCGCAT TGACAGGAAG ATTGAGTTCC CCCTGCCTGA TGAAAAGACG 
AAGAAGCGCA TCTTTCAGAT TCACACAAGC AGGATGACGC TGGCTGATGA TGTAACCCTG 
GACGACCTGA TCATGGCTAA AGATGACCTC TCTGGTGCTG ACATCAAGGC AATCTGTACA 
GAAGCTGGTC TGATGGCCTT AAGAGAACGT AGAATGAAAG TAACAAATGA AGACTTCAAA 
AAATCTAAAG AAAATGTTCT TTATAAGAAA CAGGAAGGCA CCCCTGAGGG GCTGTATCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMC1 ( NM_001330212.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317141.1 Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu85004
Clone ID Related Accession (Same CDS sequence)	NM_001330212.1 , XM_017021470.1
Accession Version	NM_001330212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355074.5, AL161662.6 and BQ574737.1. On Aug 19, 2016 this sequence version replaced XM_017021471.1. Transcript Variant: This variant (2) differs in the 5' structure, resulting in the use of a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.67226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAAG AATTCATTAG AAATCAGGAA CAAATGAAAC CATTAGAAGA AAAGCAAGAG 
GAGGAAAGAT CAAAAGTGGA TGATCTGAGG GGGACCCCGA TGTCAGTAGG AACCTTGGAA 
GAGATCATTG ATGACAATCA TGCCATCGTG TCTACATCTG TGGGCTCAGA ACACTACGTC 
AGCATTCTTT CATTTGTAGA CAAGGATCTG CTGGAACCTG GCTGCTCGGT CCTGCTCAAC 
CACAAGGTGC ATGCCGTGAT AGGGGTGCTG ATGGATGACA CGGATCCCCT GGTCACAGTG 
ATGAAGGTAG AAAAGGCCCC CCAGGAGACC TATGCAGATA TTGGGGGGTT GGACAACCAA 
ATTCAGGAAA TTAAGGAATC TGTGGAGCTT CCTCTCACCC ATCCTGAATA TTATGAAGAG 
ATGGGTATAA AGCCTCCTAA GGGGGTCATT CTCTATGGTC CACCTGGCAC AGGTAAAACC 
TTGTTAGCCA AAGCAGTAGC AAACCAAACC TCAGCCACTT TCTTGAGAGT GGTTGGCTCT 
GAACTTATTC AGAAGTACCT AGGTGATGGG CCCAAACTCG TACGGGAATT GTTCCGAGTT 
GCTGAAGAAC ATGCACCGTC CATCGTGTTT ATTGATGAAA TTGACGCCAT TGGGACAAAA 
AGATATGACT CCAATTCTGG TGGTGAGAGA GAAATTCAGC GAACAATGTT GGAACTGCTG 
AACCAGTTGG ATGGATTTGA TTCTAGGGGA GATGTGAAAG TTATCATGGC CACAAACCGA 
ATAGAAACTT TGGATCCAGC ACTTATCAGA CCAGGCCGCA TTGACAGGAA GATTGAGTTC 
CCCCTGCCTG ATGAAAAGAC GAAGAAGCGC ATCTTTCAGA TTCACACAAG CAGGATGACG 
CTGGCTGATG ATGTAACCCT GGACGACCTG ATCATGGCTA AAGATGACCT CTCTGGTGCT 
GACATCAAGG CAATCTGTAC AGAAGCTGGT CTGATGGCCT TAAGAGAACG TAGAATGAAA 
GTAACAAATG AAGACTTCAA AAAATCTAAA GAAAATGTTC TTTATAAGAA ACAGGAAGGC 
ACCCCTGAGG GGCTGTATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317141.1
CDS	349..1452
Translation	MEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Target ORF information:

RefSeq Version	NM_001330212.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330212.1

ORF Insert Sequence:

ATGGAGGAAG AATTCATTAG AAATCAGGAA CAAATGAAAC CATTAGAAGA AAAGCAAGAG 
GAGGAAAGAT CAAAAGTGGA TGATCTGAGG GGGACCCCGA TGTCAGTAGG AACCTTGGAA 
GAGATCATTG ATGACAATCA TGCCATCGTG TCTACATCTG TGGGCTCAGA ACACTACGTC 
AGCATTCTTT CATTTGTAGA CAAGGATCTG CTGGAACCTG GCTGCTCGGT CCTGCTCAAC 
CACAAGGTGC ATGCCGTGAT AGGGGTGCTG ATGGATGACA CGGATCCCCT GGTCACAGTG 
ATGAAGGTAG AAAAGGCCCC CCAGGAGACC TATGCAGATA TTGGGGGGTT GGACAACCAA 
ATTCAGGAAA TTAAGGAATC TGTGGAGCTT CCTCTCACCC ATCCTGAATA TTATGAAGAG 
ATGGGTATAA AGCCTCCTAA GGGGGTCATT CTCTATGGTC CACCTGGCAC AGGTAAAACC 
TTGTTAGCCA AAGCAGTAGC AAACCAAACC TCAGCCACTT TCTTGAGAGT GGTTGGCTCT 
GAACTTATTC AGAAGTACCT AGGTGATGGG CCCAAACTCG TACGGGAATT GTTCCGAGTT 
GCTGAAGAAC ATGCACCGTC CATCGTGTTT ATTGATGAAA TTGACGCCAT TGGGACAAAA 
AGATATGACT CCAATTCTGG TGGTGAGAGA GAAATTCAGC GAACAATGTT GGAACTGCTG 
AACCAGTTGG ATGGATTTGA TTCTAGGGGA GATGTGAAAG TTATCATGGC CACAAACCGA 
ATAGAAACTT TGGATCCAGC ACTTATCAGA CCAGGCCGCA TTGACAGGAA GATTGAGTTC 
CCCCTGCCTG ATGAAAAGAC GAAGAAGCGC ATCTTTCAGA TTCACACAAG CAGGATGACG 
CTGGCTGATG ATGTAACCCT GGACGACCTG ATCATGGCTA AAGATGACCT CTCTGGTGCT 
GACATCAAGG CAATCTGTAC AGAAGCTGGT CTGATGGCCT TAAGAGAACG TAGAATGAAA 
GTAACAAATG AAGACTTCAA AAAATCTAAA GAAAATGTTC TTTATAAGAA ACAGGAAGGC 
ACCCCTGAGG GGCTGTATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMC1 ( XM_017021470.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876959.1 Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant X1, mRNA.

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CloneID	OHu85004
Clone ID Related Accession (Same CDS sequence)	NM_001330212.1 , XM_017021470.1
Accession Version	XM_017021470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	26S proteasome regulatory subunit 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG AATTCATTAG AAATCAGGAA CAAATGAAAC CATTAGAAGA AAAGCAAGAG 
GAGGAAAGAT CAAAAGTGGA TGATCTGAGG GGGACCCCGA TGTCAGTAGG AACCTTGGAA 
GAGATCATTG ATGACAATCA TGCCATCGTG TCTACATCTG TGGGCTCAGA ACACTACGTC 
AGCATTCTTT CATTTGTAGA CAAGGATCTG CTGGAACCTG GCTGCTCGGT CCTGCTCAAC 
CACAAGGTGC ATGCCGTGAT AGGGGTGCTG ATGGATGACA CGGATCCCCT GGTCACAGTG 
ATGAAGGTAG AAAAGGCCCC CCAGGAGACC TATGCAGATA TTGGGGGGTT GGACAACCAA 
ATTCAGGAAA TTAAGGAATC TGTGGAGCTT CCTCTCACCC ATCCTGAATA TTATGAAGAG 
ATGGGTATAA AGCCTCCTAA GGGGGTCATT CTCTATGGTC CACCTGGCAC AGGTAAAACC 
TTGTTAGCCA AAGCAGTAGC AAACCAAACC TCAGCCACTT TCTTGAGAGT GGTTGGCTCT 
GAACTTATTC AGAAGTACCT AGGTGATGGG CCCAAACTCG TACGGGAATT GTTCCGAGTT 
GCTGAAGAAC ATGCACCGTC CATCGTGTTT ATTGATGAAA TTGACGCCAT TGGGACAAAA 
AGATATGACT CCAATTCTGG TGGTGAGAGA GAAATTCAGC GAACAATGTT GGAACTGCTG 
AACCAGTTGG ATGGATTTGA TTCTAGGGGA GATGTGAAAG TTATCATGGC CACAAACCGA 
ATAGAAACTT TGGATCCAGC ACTTATCAGA CCAGGCCGCA TTGACAGGAA GATTGAGTTC 
CCCCTGCCTG ATGAAAAGAC GAAGAAGCGC ATCTTTCAGA TTCACACAAG CAGGATGACG 
CTGGCTGATG ATGTAACCCT GGACGACCTG ATCATGGCTA AAGATGACCT CTCTGGTGCT 
GACATCAAGG CAATCTGTAC AGAAGCTGGT CTGATGGCCT TAAGAGAACG TAGAATGAAA 
GTAACAAATG AAGACTTCAA AAAATCTAAA GAAAATGTTC TTTATAAGAA ACAGGAAGGC 
ACCCCTGAGG GGCTGTATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876959.1
CDS	1787..2890
Translation	MEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Target ORF information:

RefSeq Version	XM_017021470.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 26S subunit, ATPase 1 (PSMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021470.1

ORF Insert Sequence:

ATGGAGGAAG AATTCATTAG AAATCAGGAA CAAATGAAAC CATTAGAAGA AAAGCAAGAG 
GAGGAAAGAT CAAAAGTGGA TGATCTGAGG GGGACCCCGA TGTCAGTAGG AACCTTGGAA 
GAGATCATTG ATGACAATCA TGCCATCGTG TCTACATCTG TGGGCTCAGA ACACTACGTC 
AGCATTCTTT CATTTGTAGA CAAGGATCTG CTGGAACCTG GCTGCTCGGT CCTGCTCAAC 
CACAAGGTGC ATGCCGTGAT AGGGGTGCTG ATGGATGACA CGGATCCCCT GGTCACAGTG 
ATGAAGGTAG AAAAGGCCCC CCAGGAGACC TATGCAGATA TTGGGGGGTT GGACAACCAA 
ATTCAGGAAA TTAAGGAATC TGTGGAGCTT CCTCTCACCC ATCCTGAATA TTATGAAGAG 
ATGGGTATAA AGCCTCCTAA GGGGGTCATT CTCTATGGTC CACCTGGCAC AGGTAAAACC 
TTGTTAGCCA AAGCAGTAGC AAACCAAACC TCAGCCACTT TCTTGAGAGT GGTTGGCTCT 
GAACTTATTC AGAAGTACCT AGGTGATGGG CCCAAACTCG TACGGGAATT GTTCCGAGTT 
GCTGAAGAAC ATGCACCGTC CATCGTGTTT ATTGATGAAA TTGACGCCAT TGGGACAAAA 
AGATATGACT CCAATTCTGG TGGTGAGAGA GAAATTCAGC GAACAATGTT GGAACTGCTG 
AACCAGTTGG ATGGATTTGA TTCTAGGGGA GATGTGAAAG TTATCATGGC CACAAACCGA 
ATAGAAACTT TGGATCCAGC ACTTATCAGA CCAGGCCGCA TTGACAGGAA GATTGAGTTC 
CCCCTGCCTG ATGAAAAGAC GAAGAAGCGC ATCTTTCAGA TTCACACAAG CAGGATGACG 
CTGGCTGATG ATGTAACCCT GGACGACCTG ATCATGGCTA AAGATGACCT CTCTGGTGCT 
GACATCAAGG CAATCTGTAC AGAAGCTGGT CTGATGGCCT TAAGAGAACG TAGAATGAAA 
GTAACAAATG AAGACTTCAA AAAATCTAAA GAAAATGTTC TTTATAAGAA ACAGGAAGGC 
ACCCCTGAGG GGCTGTATCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene.
PloS one11(2)e0148678(2016)
Czub B,Shah AZ,Alfano G,Kruczek PM,Chakarova CF,Bhattacharya SS

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

PSMC1 Gene in Parkinson's Disease.
European neurology68(4)193-8(2012)
G?mez-Garre P,Jes?s S,Carrillo F,C?ceres-Redondo MT,Bernal-Bernal I,Carballo M,Gao L,Mir P

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PSMC1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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