PRDM9 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRDM9
Entrez Gene ID	56979
Full Name	PR/SET domain 9
Synonyms	KMT8B,MEISETZ,MSBP3,PFM6,ZNF899
General protein information	Preferred Names PR/SET domain 9 Names histone-lysine N-methyltransferase PRDM9 PR domain 9 PR domain containing 9 PR domain zinc finger protein 9 histone methyl transferase minisatellite binding protein 3 (115kD)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p14.2
Summary	The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015].
Disorder MIM:	609760

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310214.1	NP_001297143.1	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 A
NM_001376900.1	NP_001363829.1	histone-lysine N-methyltransferase PRDM9 PRDM9 C
NM_001310214.2	NP_001297143.1	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 A
NM_020227.3	NP_064612.2	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
NM_020227.4	NP_064612.2	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prdm9	NP_659058.2
Homo sapiens (human)	PRDM9	NP_064612.2
Pan troglodytes (chimpanzee)	PRDM9	XP_517829.3
Bos taurus (cattle)	LOC100851938	XP_005228562.1
Rattus norvegicus (Norway rat)	Prdm9	NP_001102373.2
	LOC695034	XP_001083675.2
Bos taurus (cattle)	PRDM7	XP_002683924.2

Gene Ontology
Bibliography

Related articles in PubMed

DNA recombination. Recombination initiation maps of individual human genomes.
Pratto F, Brick K, Khil P, Smagulova F, Petukhova GV, Camerini-Otero RD
Science (New York, N.Y.)346(6211)1256442(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The most common genetic variant of PRDM9 is allele A (PRDM9a) which contains 13 Cys2-His2 zinc fingers (ZF); the second-most common variant among African populations is allele C (PRDM9c), which contains 14 Cys2-His2 ZF. Data suggest that Ser764 in ZF9 allows PRDM9c to accommodate a variable base, whereas PRDM9a Arg764 recognizes a conserved guanine.
Title: Structural basis of human PR/SET domain 9 (PRDM9) allele C-specific recognition of its cognate DNA sequence.

The article outlines the role of PRDM9 in fertility.
Title: X marks the spot: PRDM9 rescues hybrid sterility by finding hidden treasure in the genome.

Allele C was found to bind a C-specific hot spot with higher affinity than allele A bound A-specific hot spots
Title: Structural basis for human PRDM9 action at recombination hot spots.

subspecies-specific degradation of PRDM9 binding sites by meiotic drive, which steadily increases asymmetric PRDM9 binding, has impacts beyond simply changing hotspot positions, and strongly support a direct involvement in hybrid infertility
Title: Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice.

PRDM9 protein variants form functional heteromeric complexes which can bind hotspots sequences. When a heteromeric complex binds at a hotspot of one PRDM9 variant, the other PRDM9 variant, which would otherwise not bind, methylates hotspot nucleosomes
Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots.

The following PRDM9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRDM9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03253	NM_001376900.1 Latest version!	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu03253	NM_020227.4 Latest version!	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu70215	NM_001310214.2 Latest version!	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu70215	NM_001310214.1	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu03253	NM_020227.3	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRDM9 ( NM_001376900.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363829.1 Homo sapiens PR/SET domain 9 (PRDM9), transcript variant C, mRNA.

Price & Availability↑

CloneID	OHu03253
Clone ID Related Accession (Same CDS sequence)	NM_001376900.1 , NM_020227.3 , NM_020227.4
Accession Version	NM_001376900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase PRDM9 PRDM9 C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025451.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.208875.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
ACTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363829.1
CDS	258..2942
Translation	MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITIGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKMYSLRERKGHAYKEVSEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWLITKGRNCYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVITHQRTHTGEKLYVCRECGRGFSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTHTGEKPYVCRECGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE

Target ORF information:

RefSeq Version	NM_001376900.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376900.1

ORF Insert Sequence:

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
ACTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRDM9 ( NM_020227.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_064612.2 Homo sapiens PR/SET domain 9 (PRDM9), transcript variant B, mRNA.

Price & Availability↑

CloneID	OHu03253
Clone ID Related Accession (Same CDS sequence)	NM_001376900.1 , NM_020227.3 , NM_020227.4
Accession Version	NM_020227.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025451.6. On Nov 22, 2019 this sequence version replaced NM_020227.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ388610.1, AK301776.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
ACTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064612.2
CDS	189..2873
Translation	MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITIGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKMYSLRERKGHAYKEVSEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWLITKGRNCYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVITHQRTHTGEKLYVCRECGRGFSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTHTGEKPYVCRECGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE

Target ORF information:

RefSeq Version	NM_020227.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020227.4

ORF Insert Sequence:

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
ACTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRDM9 ( NM_001310214.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001297143.1 Homo sapiens PR/SET domain 9 (PRDM9), transcript variant A, mRNA.

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CloneID	OHu70215
Clone ID Related Accession (Same CDS sequence)	NM_001310214.1 , NM_001310214.2
Accession Version	NM_001310214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025451.6 and AK301776.1. On Nov 22, 2019 this sequence version replaced NM_001310214.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ388610.1, AK301776.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
AGTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297143.1
CDS	189..2873
Translation	MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITIGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKMYSLRERKGHAYKEVSEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWLITKGRNCYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVITHQRTHTGEKLYVCRECGRGFSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSWQSVLLSHQRTHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTHTGEKPYVCRECGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE

Target ORF information:

RefSeq Version	NM_001310214.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310214.2

ORF Insert Sequence:

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
AGTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRDM9 ( NM_020227.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_064612.2 Homo sapiens PR/SET domain 9 (PRDM9), transcript variant B, mRNA.

Price & Availability↑

CloneID	OHu03253
Clone ID Related Accession (Same CDS sequence)	NM_001376900.1 , NM_020227.3 , NM_020227.4
Accession Version	NM_020227.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB636359.1, AC025451.6 and DQ388610.1. On Jul 8, 2015 this sequence version replaced NM_020227.2. Transcript Variant: This variant (PRDM9 B allele, PMID:20044539) matches the reference genome sequence and encodes isoform PRDM9 B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ388610.1, AK301776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
ACTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064612.2
CDS	189..2873
Translation	MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITIGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKMYSLRERKGHAYKEVSEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWLITKGRNCYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVITHQRTHTGEKLYVCRECGRGFSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTHTGEKPYVCRECGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE

Target ORF information:

RefSeq Version	NM_020227.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020227.3

ORF Insert Sequence:

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
ACTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRDM9 ( NM_001310214.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001297143.1 Homo sapiens PR/SET domain 9 (PRDM9), transcript variant A, mRNA.

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CloneID	OHu70215
Clone ID Related Accession (Same CDS sequence)	NM_001310214.1 , NM_001310214.2
Accession Version	NM_001310214.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Homo sapiens(human)
Product	histone-lysine N-methyltransferase PRDM9 isoform PRDM9 A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK301776.1. Transcript Variant: This variant (PRDM9 A allele, PMID:20044539) differs in the 5' and 3' UTRs, and contains a single nucleotide difference in the CDS, compared to the PRDM9 B allele. It encodes PRDM9 A, an isoform with a serine at amino acid 681, rather than the threonine found at this position in the PRDM9 B isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301776.1, DQ388610.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
AGTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297143.1
CDS	143..2827
Translation	MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITIGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMPKASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKMYSLRERKGHAYKEVSEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWLITKGRNCYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDEYGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPSARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVITHQRTHTGEKLYVCRECGRGFSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSWQSVLLSHQRTHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTHTGEKPYVCRECGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE

Target ORF information:

RefSeq Version	NM_001310214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PR/SET domain 9 (PRDM9), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310214.1

ORF Insert Sequence:

ATGAGCCCTG AAAAGTCCCA AGAGGAGAGC CCAGAAGAAG ACACAGAGAG AACAGAGCGG 
AAGCCCATGG TCAAAGATGC CTTCAAAGAC ATTTCCATAT ACTTCACCAA GGAAGAATGG 
GCAGAGATGG GAGACTGGGA GAAAACTCGC TATAGGAATG TGAAAAGGAA CTATAATGCA 
CTGATTACTA TAGGTCTCAG AGCCACTCGA CCAGCTTTCA TGTGTCACCG AAGGCAGGCC 
ATCAAACTCC AGGTGGATGA CACAGAAGAT TCTGATGAAG AATGGACCCC TAGGCAGCAA 
GTCAAACCTC CTTGGATGGC CTTAAGAGTG GAACAGCGTA AACACCAGAA GGGAATGCCC 
AAGGCGTCAT TCAGTAATGA ATCTAGTTTG AAAGAATTGT CAAGAACAGC AAATTTACTG 
AATGCAAGTG GCTCAGAGCA GGCTCAGAAA CCAGTGTCCC CTTCTGGAGA AGCAAGTACC 
TCTGGACAGC ACTCAAGACT AAAACTGGAA CTCAGGAAGA AGGAGACTGA AAGAAAGATG 
TATAGCCTGC GAGAAAGAAA GGGTCATGCA TACAAAGAGG TCAGCGAGCC GCAGGATGAT 
GATTACCTCT ATTGTGAGAT GTGTCAGAAC TTCTTCATTG ACAGCTGTGC TGCCCATGGG 
CCCCCTACAT TTGTAAAGGA CAGTGCAGTG GACAAGGGGC ACCCCAACCG TTCAGCCCTC 
AGTCTGCCCC CAGGGCTGAG AATTGGGCCA TCAGGCATCC CTCAGGCTGG GCTTGGAGTA 
TGGAATGAGG CATCTGATCT GCCGCTGGGT CTGCACTTTG GCCCTTATGA GGGCCGAATT 
ACAGAAGACG AAGAGGCAGC CAACAATGGA TACTCCTGGC TGATCACCAA GGGGAGAAAC 
TGCTATGAGT ATGTGGATGG AAAAGATAAA TCCTGGGCCA ACTGGATGAG GTATGTGAAC 
TGTGCCCGGG ATGATGAAGA GCAGAACCTG GTGGCCTTCC AGTACCACAG GCAGATCTTC 
TATAGAACCT GCCGAGTCAT TAGGCCAGGC TGTGAACTGC TGGTCTGGTA TGGGGATGAA 
TACGGCCAGG AACTGGGCAT CAAGTGGGGC AGCAAGTGGA AGAAAGAGCT CATGGCAGGG 
AGAGAACCAA AGCCAGAGAT CCATCCATGT CCCTCATGCT GTCTGGCCTT TTCAAGTCAG 
AAATTTCTCA GTCAACATGT AGAACGCAAT CACTCCTCTC AGAACTTCCC AGGACCATCT 
GCAAGAAAAC TCCTCCAACC AGAGAATCCC TGCCCAGGGG ATCAGAATCA GGAGCAGCAA 
TATCCAGATC CACACAGCCG TAATGACAAA ACCAAAGGTC AAGAGATCAA AGAAAGGTCC 
AAACTCTTGA ATAAAAGGAC ATGGCAGAGG GAGATTTCAA GGGCCTTTTC TAGCCCACCC 
AAAGGACAAA TGGGGAGCTG TAGAGTGGGA AAAAGAATAA TGGAAGAAGA GTCCAGAACA 
GGCCAGAAAG TGAATCCAGG GAACACAGGC AAATTATTTG TGGGGGTAGG AATCTCAAGA 
ATTGCAAAAG TCAAGTATGG AGAGTGTGGA CAAGGTTTCA GTGTTAAATC AGATGTTATT 
ACACACCAAA GGACACATAC AGGGGAGAAG CTCTACGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGA AGTCACACCT CCTCATTCAC CAGAGGATAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC TGGCAGTCAG TCCTCCTCAC TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TAGCCGGCAG 
TCAGTCCTCC TCACTCACCA GAGGAGACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGCCG GCAGTCAGTC CTCCTCACTC ACCAGAGGAG ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCTGGCAGTC AGTCCTCCTC 
AGTCACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTAGCTGGC AGTCAGTCCT CCTCACTCAC CAGAGGACAC ACACAGGGGA GAAGCCCTAT 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC AATAAGTCAC ACCTCCTCAG ACACCAGAGG 
ACACACACAG GGGAGAAGCC CTATGTCTGC AGGGAGTGTG GGCGGGGCTT TCGCGATAAG 
TCACACCTCC TCAGACACCA GAGGACACAC ACAGGGGAGA AGCCCTATGT CTGCAGGGAG 
TGTGGGCGGG GCTTTAGAGA TAAGTCAAAC CTCCTCAGTC ACCAGAGGAC ACACACAGGG 
GAGAAGCCCT ATGTCTGCAG GGAGTGTGGG CGGGGCTTTA GCAATAAGTC ACACCTCCTC 
AGACACCAGA GGACACACAC AGGGGAGAAG CCCTATGTCT GCAGGGAGTG TGGGCGGGGC 
TTTCGCAATA AGTCACACCT CCTCAGACAC CAGAGGACAC ACACAGGGGA GAAGCCCTAC 
GTCTGCAGGG AGTGTGGGCG GGGCTTTAGC GATAGGTCAA GCCTCTGCTA TCACCAGAGG 
ACACACACAG GGGAGAAGCC CTACGTCTGC AGGGAGGATG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA recombination. Recombination initiation maps of individual human genomes.
Science (New York, N.Y.)346(6211)1256442(2014 Nov)
Pratto F,Brick K,Khil P,Smagulova F,Petukhova GV,Camerini-Otero RD

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PRDM9 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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