PSMB4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMB4
Entrez Gene ID	5692
Full Name	proteasome subunit beta 4
Synonyms	HN3,HsN3,PROS-26,PROS26
General protein information	Preferred Names proteasome subunit beta 4 Names proteasome subunit beta type-4 26 kDa prosomal protein hsBPROS26 macropain beta chain multicatalytic endopeptidase complex beta chain proteasome (prosome, macropain) subunit, beta type, 4 proteasome beta chain proteasome chain 3 proteasome subunit HsN3 proteasome subunit, beta type, 4 testis tissue sperm-binding protein Li 79P
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Summary	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul 2008].
Disorder MIM:	602177

mRNA and Protein(s)

mRNA	Protein	Name
NM_002796.2	NP_002787.2	proteasome subunit beta type-4
NM_002796.3	NP_002787.2	proteasome subunit beta type-4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PSMB4	XP_003949593.1
Rattus norvegicus (Norway rat)	Psmb4	NP_113817.1
Drosophila melanogaster (fruit fly)	Prosbeta7	NP_649529.1
Neurospora crassa	NCU07365	XP_957993.1
Arabidopsis thaliana (thale cress)	PBG1	NP_176040.1
Xenopus tropicalis (tropical clawed frog)	psmb4	NP_001011182.1
	PSMB4	XP_002801820.1
Schizosaccharomyces pombe (fission yeast)	SPBC577.10	NP_595308.1
Canis lupus familiaris (dog)	PSMB4	XP_533057.3
Mus musculus (house mouse)	Psmb4	NP_032971.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011444	XP_317860.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012608	XP_306986.3
Magnaporthe oryzae (rice blast fungus)	MGG_00331	XP_003718754.1
Kluyveromyces lactis	KLLA0D11506g	XP_453574.1
Eremothecium gossypii	AGOS_AFR258W	NP_985805.1
Homo sapiens (human)	PSMB4	NP_002787.2
Bos taurus (cattle)	PSMB4	NP_001029438.1
Gallus gallus (chicken)	PSMB4	XP_427542.4
Danio rerio (zebrafish)	psmb4	NP_001017801.1
Saccharomyces cerevisiae (baker's yeast)	PRE4	NP_116708.1
Oryza sativa (rice)	Os09g0515200	NP_001063665.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b.
Zhang X, Lin D, Lin Y, Chen H, Zou M, Zhong S, Yi X, Han S
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317705767(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production.
Brehm A, Liu Y, Sheikh A, Marrero B, Omoyinmi E, Zhou Q, Montealegre G, Biancotto A, Reinhardt A, Almeida de Jesus A, Pelletier M, Tsai WL, Remmers EF, Kardava L, Hill S, Kim H, Lachmann HJ, Megarbane A, Chae JJ, Brady J, Castillo RD, Brown D, Casano AV, Gao L, Chapelle D, Huang Y, Stone D, Chen Y, Sotzny F, Lee CC, Kastner DL, Torrelo A, Zlotogorski A, Moir S, Gadina M, McCoy P, Wesley R, Rother KI, Rother K, Hildebrand PW, Brogan P, Kr?ger E, Aksentijevich I, Goldbach-Mansky R
The Journal of clinical investigation125(11)4196-211(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We further identified miR-148b as a negative regulator of proteasome beta-4 subunit. Enforced expression of miR-148b resulted in vitro growth inhibition of melanoma cells, whereas this inhibition was further abolished by enforced expression of proteasome beta-4 subunit
Title: Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b.

Our findings implied that PSMB4 is involved in the progression of EOC (epithelial ovarian cancer) and could serve as potential therapeutical target of EOC. These data suggested that PSMB4 may promote cell proliferation via the NF-kappaB-target gene in EOC (epithelial ovarian cancer)
Title: PSMB4 expression associates with epithelial ovarian cancer growth and poor prognosis.

Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-kappaB-miR-21 signaling, which may represent promising targets for novel specific therapies.
Title: PSMB4 promotes multiple myeloma cell growth by activating NF-?B-miR-21 signaling.

Data indicate that recruitment of proteasomes into mutant huntingtin (mHTT) protein initiated inclusion bodies (IBs) was observed when the PSMB4-GFP was co-expressed with mHTT.
Title: Dynamic recruitment of active proteasomes into polyglutamine initiated inclusion bodies.

proteasome subunit beta type 4 (PSMB4), the beta7 subunit of the 20S core complex, was identified as a direct binding partner of CRBN.
Title: Cereblon inhibits proteasome activity by binding to the 20S core proteasome subunit beta type 4.

The following PSMB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13170	NM_002796.3 Latest version!	Homo sapiens proteasome 20S subunit beta 4 (PSMB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13170	NM_002796.2	Homo sapiens proteasome subunit beta 4 (PSMB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMB4 ( NM_002796.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002787.2 Homo sapiens proteasome subunit beta 4 (PSMB4), mRNA.

Price & Availability↑

CloneID	OHu13170
Clone ID Related Accession (Same CDS sequence)	NM_002796.2 , NM_002796.3
Accession Version	NM_002796.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D26600.1. On Aug 29, 2002 this sequence version replaced NM_002796.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D26600.1, HM005586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGT TTTTGGGGTC GCGGTCCGGA CTTTGGGCGG GGGGTCCGGC CCCAGGACAG 
TTTTACCGCA TTCCGTCCAC TCCCGATTCC TTCATGGATC CGGCGTCTGC ACTTTACAGA 
GGTCCAATCA CGCGGACCCA GAACCCCATG GTGACCGGGA CCTCAGTCCT CGGCGTTAAG 
TTCGAGGGCG GAGTGGTGAT TGCCGCAGAC ATGCTGGGAT CCTACGGCTC CTTGGCTCGT 
TTCCGCAACA TCTCTCGCAT TATGCGAGTC AACAACAGTA CCATGCTGGG TGCCTCTGGC 
GACTACGCTG ATTTCCAGTA TTTGAAGCAA GTTCTCGGCC AGATGGTGAT TGATGAGGAG 
CTTCTGGGAG ATGGACACAG CTATAGTCCT AGAGCTATTC ATTCATGGCT GACCAGGGCC 
ATGTACAGCC GGCGCTCGAA GATGAACCCT TTGTGGAACA CCATGGTCAT CGGAGGCTAT 
GCTGATGGAG AGAGCTTCCT CGGTTATGTG GACATGCTTG GTGTAGCCTA TGAAGCCCCT 
TCGCTGGCCA CTGGTTATGG TGCATACTTG GCTCAGCCTC TGCTGCGAGA AGTTCTGGAG 
AAGCAGCCAG TGCTAAGCCA GACCGAGGCC CGCGACTTAG TAGAACGCTG CATGCGAGTG 
CTGTACTACC GAGATGCCCG TTCTTACAAC CGGTTTCAAA TCGCCACTGT CACCGAAAAA 
GGTGTTGAAA TAGAGGGACC ATTGTCTACA GAGACCAACT GGGATATTGC CCACATGATC 
AGTGGCTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002787.2
CDS	24..818
Translation	MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIAHMISGFE

Target ORF information:

RefSeq Version	NM_002796.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome subunit beta 4 (PSMB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002796.2

ORF Insert Sequence:

ATGGAAGCGT TTTTGGGGTC GCGGTCCGGA CTTTGGGCGG GGGGTCCGGC CCCAGGACAG 
TTTTACCGCA TTCCGTCCAC TCCCGATTCC TTCATGGATC CGGCGTCTGC ACTTTACAGA 
GGTCCAATCA CGCGGACCCA GAACCCCATG GTGACCGGGA CCTCAGTCCT CGGCGTTAAG 
TTCGAGGGCG GAGTGGTGAT TGCCGCAGAC ATGCTGGGAT CCTACGGCTC CTTGGCTCGT 
TTCCGCAACA TCTCTCGCAT TATGCGAGTC AACAACAGTA CCATGCTGGG TGCCTCTGGC 
GACTACGCTG ATTTCCAGTA TTTGAAGCAA GTTCTCGGCC AGATGGTGAT TGATGAGGAG 
CTTCTGGGAG ATGGACACAG CTATAGTCCT AGAGCTATTC ATTCATGGCT GACCAGGGCC 
ATGTACAGCC GGCGCTCGAA GATGAACCCT TTGTGGAACA CCATGGTCAT CGGAGGCTAT 
GCTGATGGAG AGAGCTTCCT CGGTTATGTG GACATGCTTG GTGTAGCCTA TGAAGCCCCT 
TCGCTGGCCA CTGGTTATGG TGCATACTTG GCTCAGCCTC TGCTGCGAGA AGTTCTGGAG 
AAGCAGCCAG TGCTAAGCCA GACCGAGGCC CGCGACTTAG TAGAACGCTG CATGCGAGTG 
CTGTACTACC GAGATGCCCG TTCTTACAAC CGGTTTCAAA TCGCCACTGT CACCGAAAAA 
GGTGTTGAAA TAGAGGGACC ATTGTCTACA GAGACCAACT GGGATATTGC CCACATGATC 
AGTGGCTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB4 ( NM_002796.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002787.2 Homo sapiens proteasome 20S subunit beta 4 (PSMB4), mRNA.

Price & Availability↑

CloneID	OHu13170
Clone ID Related Accession (Same CDS sequence)	NM_002796.2 , NM_002796.3
Accession Version	NM_002796.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL589764.19. On Nov 25, 2018 this sequence version replaced NM_002796.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.658846.1, SRR1163655.692484.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290541.7/ ENSP00000290541.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGCGT TTTTGGGGTC GCGGTCCGGA CTTTGGGCGG GGGGTCCGGC CCCAGGACAG 
TTTTACCGCA TTCCGTCCAC TCCCGATTCC TTCATGGATC CGGCGTCTGC ACTTTACAGA 
GGTCCAATCA CGCGGACCCA GAACCCCATG GTGACCGGGA CCTCAGTCCT CGGCGTTAAG 
TTCGAGGGCG GAGTGGTGAT TGCCGCAGAC ATGCTGGGAT CCTACGGCTC CTTGGCTCGT 
TTCCGCAACA TCTCTCGCAT TATGCGAGTC AACAACAGTA CCATGCTGGG TGCCTCTGGC 
GACTACGCTG ATTTCCAGTA TTTGAAGCAA GTTCTCGGCC AGATGGTGAT TGATGAGGAG 
CTTCTGGGAG ATGGACACAG CTATAGTCCT AGAGCTATTC ATTCATGGCT GACCAGGGCC 
ATGTACAGCC GGCGCTCGAA GATGAACCCT TTGTGGAACA CCATGGTCAT CGGAGGCTAT 
GCTGATGGAG AGAGCTTCCT CGGTTATGTG GACATGCTTG GTGTAGCCTA TGAAGCCCCT 
TCGCTGGCCA CTGGTTATGG TGCATACTTG GCTCAGCCTC TGCTGCGAGA AGTTCTGGAG 
AAGCAGCCAG TGCTAAGCCA GACCGAGGCC CGCGACTTAG TAGAACGCTG CATGCGAGTG 
CTGTACTACC GAGATGCCCG TTCTTACAAC CGGTTTCAAA TCGCCACTGT CACCGAAAAA 
GGTGTTGAAA TAGAGGGACC ATTGTCTACA GAGACCAACT GGGATATTGC CCACATGATC 
AGTGGCTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002787.2
CDS	16..810
Translation	MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIAHMISGFE

Target ORF information:

RefSeq Version	NM_002796.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 20S subunit beta 4 (PSMB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002796.3

ORF Insert Sequence:

ATGGAAGCGT TTTTGGGGTC GCGGTCCGGA CTTTGGGCGG GGGGTCCGGC CCCAGGACAG 
TTTTACCGCA TTCCGTCCAC TCCCGATTCC TTCATGGATC CGGCGTCTGC ACTTTACAGA 
GGTCCAATCA CGCGGACCCA GAACCCCATG GTGACCGGGA CCTCAGTCCT CGGCGTTAAG 
TTCGAGGGCG GAGTGGTGAT TGCCGCAGAC ATGCTGGGAT CCTACGGCTC CTTGGCTCGT 
TTCCGCAACA TCTCTCGCAT TATGCGAGTC AACAACAGTA CCATGCTGGG TGCCTCTGGC 
GACTACGCTG ATTTCCAGTA TTTGAAGCAA GTTCTCGGCC AGATGGTGAT TGATGAGGAG 
CTTCTGGGAG ATGGACACAG CTATAGTCCT AGAGCTATTC ATTCATGGCT GACCAGGGCC 
ATGTACAGCC GGCGCTCGAA GATGAACCCT TTGTGGAACA CCATGGTCAT CGGAGGCTAT 
GCTGATGGAG AGAGCTTCCT CGGTTATGTG GACATGCTTG GTGTAGCCTA TGAAGCCCCT 
TCGCTGGCCA CTGGTTATGG TGCATACTTG GCTCAGCCTC TGCTGCGAGA AGTTCTGGAG 
AAGCAGCCAG TGCTAAGCCA GACCGAGGCC CGCGACTTAG TAGAACGCTG CATGCGAGTG 
CTGTACTACC GAGATGCCCG TTCTTACAAC CGGTTTCAAA TCGCCACTGT CACCGAAAAA 
GGTGTTGAAA TAGAGGGACC ATTGTCTACA GAGACCAACT GGGATATTGC CCACATGATC 
AGTGGCTTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317705767(2017 Jun)
Zhang X,Lin D,Lin Y,Chen H,Zou M,Zhong S,Yi X,Han S

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production.
The Journal of clinical investigation125(11)4196-211(2015 Nov)
Brehm A,Liu Y,Sheikh A,Marrero B,Omoyinmi E,Zhou Q,Montealegre G,Biancotto A,Reinhardt A,Almeida de Jesus A,Pelletier M,Tsai WL,Remmers EF,Kardava L,Hill S,Kim H,Lachmann HJ,Megarbane A,Chae JJ,Brady J,Castillo RD,Brown D,Casano AV,Gao L,Chapelle D,Huang Y,Stone D,Chen Y,Sotzny F,Lee CC,Kastner DL,Torrelo A,Zlotogorski A,Moir S,Gadina M,McCoy P,Wesley R,Rother KI,Rother K,Hildebrand PW,Brogan P,Kr?ger E,Aksentijevich I,Goldbach-Mansky R

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PSMB4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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