EXOSC5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EXOSC5
Entrez Gene ID	56915
Full Name	exosome component 5
Synonyms	RRP41B,RRP46,Rrp46p,hRrp46p,p12B
General protein information	Preferred Names exosome component 5 Names exosome complex component RRP46 chronic myelogenous leukemia tumor antigen 28 exosome complex exonuclease RRP46 exosome component Rrp46 ribosomal RNA-processing protein 46
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.2
Disorder MIM:	606492

mRNA and Protein(s)

mRNA	Protein	Name
NM_020158.4	NP_064543.3	exosome complex component RRP46
NM_020158.3	NP_064543.3	exosome complex component RRP46

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EXOSC5	XP_001152170.1
	EXOSC5	XP_001102687.2
Gallus gallus (chicken)	EXOSC5	XP_003643513.1
Oryza sativa (rice)	Os03g0854200	NP_001051936.1
Xenopus tropicalis (tropical clawed frog)	exosc5	XP_002939437.2
Mus musculus (house mouse)	Exosc5	NP_613052.1
Danio rerio (zebrafish)	exosc5	NP_001038817.2
Eremothecium gossypii	AGOS_AAR035C	NP_982576.2
Arabidopsis thaliana (thale cress)	AT3G46210	NP_001030818.1
Homo sapiens (human)	EXOSC5	NP_064543.3
Canis lupus familiaris (dog)	EXOSC5	XP_852838.1
Rattus norvegicus (Norway rat)	Exosc5	NP_001100963.2
Drosophila melanogaster (fruit fly)	Rrp46	NP_650001.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002177	XP_308011.5
Caenorhabditis elegans (roundworm)	crn-5	NP_496284.1
Bos taurus (cattle)	EXOSC5	XP_005219175.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA.
Wasmuth EV, Januszyk K, Lima CD
Nature511(7510)435-9(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Further, subcellular co-localization assay showed that the two proteins could co-localize in the cytoplasm of K562 cells, but WT1/CML28 complexes were not detected by using immunoprecipitation.
Title: Interaction of human genes WT1 and CML28 in leukemic cells.

Results suggest that Rrp46 forms a homodimer separately from exosome complexes and, depending on species, is a structural component of the machinery that cleaves DNA during apoptosis.
Title: Structural and biochemical characterization of CRN-5 and Rrp46: an exosome component participating in apoptotic DNA degradation.

rAAV/CML28-transduced DCs vaccine may serve as a feasible approach for the treatment of CML28-associated cancers.
Title: Activation of cytotoxic T lymphocytes against CML28-bearing tumors by dendritic cells transduced with a recombinant adeno-associated virus encoding the CML28 gene.

These results in our study indicates gene silencing of SOCS1 remarkably enhanced the cytotoxicity efficiency of CML28 DNA vaccine in DCs.
Title: Induction of CML28-specific cytotoxic T cell responses using co-transfected dendritic cells with CML28 DNA vaccine and SOCS1 small interfering RNA expression vector.

Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring.
Title: Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring.

The following EXOSC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EXOSC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03225	NM_020158.4 Latest version!	Homo sapiens exosome component 5 (EXOSC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu03225	NM_020158.3	Homo sapiens exosome component 5 (EXOSC5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EXOSC5 ( NM_020158.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_064543.3 Homo sapiens exosome component 5 (EXOSC5), mRNA.

Price & Availability↑

CloneID	OHu03225
Clone ID Related Accession (Same CDS sequence)	NM_020158.4 , NM_020158.3
Accession Version	NM_020158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	exosome complex component RRP46
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011462.4 and AF281134.1. On Nov 23, 2018 this sequence version replaced NM_020158.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF285785.1, SRR5189658.209245.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000221233.9/ ENSP00000221233.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAGG AGACGCATAC TGACGCCAAA ATCCGTGCTG AAAATGGAAC AGGGTCCAGC 
CCTCGGGGTC CTGGCTGCAG CCTCCGGCAC TTTGCCTGCG AACAGAACCT GCTGTCGCGG 
CCAGATGGCT CTGCTTCCTT CCTGCAAGGT GACACCTCTG TCCTGGCGGG TGTGTACGGG 
CCGGCCGAGG TGAAGGTCAG CAAAGAGATT TTCAACAAGG CCACACTCGA AGTGATCCTG 
AGGCCGAAGA TTGGGCTGCC TGGTGTTGCA GAGAAGAGCC GGGAGCGGCT GATCAGGAAC 
ACGTGCGAGG CGGTGGTGCT GGGCACGTTG CACCCCCGCA CCTCCATCAC CGTGGTGCTG 
CAGGTTGTCA GCGATGCCGG CTCTCTCCTG GCCTGTTGTC TGAATGCCGC CTGCATGGCA 
TTGGTGGATG CAGGTGTGCC CATGCGGGCT CTCTTCTGTG GGGTCGCCTG CGCCCTGGAC 
TCTGATGGGA CCCTCGTGCT GGATCCTACA TCCAAGCAAG AAAAGGAGGC CCGGGCAGTC 
CTGACCTTTG CCCTGGACAG CGTGGAACGG AAGCTGCTGA TGTCCAGCAC CAAGGGGCTC 
TACTCAGACA CTGAGCTCCA GCAGTGCCTG GCTGCGGCCC AGGCCGCTTC GCAACACGTC 
TTCCGTTTCT ACCGGGAATC GCTGCAGAGG CGTTACTCCA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064543.3
CDS	32..739
Translation	MEEETHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS

Target ORF information:

RefSeq Version	NM_020158.4
Organism	Homo sapiens(human)
Definition	Homo sapiens exosome component 5 (EXOSC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020158.4

ORF Insert Sequence:

ATGGAGGAGG AGACGCATAC TGACGCCAAA ATCCGTGCTG AAAATGGAAC AGGGTCCAGC 
CCTCGGGGTC CTGGCTGCAG CCTCCGGCAC TTTGCCTGCG AACAGAACCT GCTGTCGCGG 
CCAGATGGCT CTGCTTCCTT CCTGCAAGGT GACACCTCTG TCCTGGCGGG TGTGTACGGG 
CCGGCCGAGG TGAAGGTCAG CAAAGAGATT TTCAACAAGG CCACACTCGA AGTGATCCTG 
AGGCCGAAGA TTGGGCTGCC TGGTGTTGCA GAGAAGAGCC GGGAGCGGCT GATCAGGAAC 
ACGTGCGAGG CGGTGGTGCT GGGCACGTTG CACCCCCGCA CCTCCATCAC CGTGGTGCTG 
CAGGTTGTCA GCGATGCCGG CTCTCTCCTG GCCTGTTGTC TGAATGCCGC CTGCATGGCA 
TTGGTGGATG CAGGTGTGCC CATGCGGGCT CTCTTCTGTG GGGTCGCCTG CGCCCTGGAC 
TCTGATGGGA CCCTCGTGCT GGATCCTACA TCCAAGCAAG AAAAGGAGGC CCGGGCAGTC 
CTGACCTTTG CCCTGGACAG CGTGGAACGG AAGCTGCTGA TGTCCAGCAC CAAGGGGCTC 
TACTCAGACA CTGAGCTCCA GCAGTGCCTG GCTGCGGCCC AGGCCGCTTC GCAACACGTC 
TTCCGTTTCT ACCGGGAATC GCTGCAGAGG CGTTACTCCA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EXOSC5 ( NM_020158.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_064543.3 Homo sapiens exosome component 5 (EXOSC5), mRNA.

Price & Availability↑

CloneID	OHu03225
Clone ID Related Accession (Same CDS sequence)	NM_020158.4 , NM_020158.3
Accession Version	NM_020158.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	exosome complex component RRP46
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF281134.1 and BU683254.1. On May 14, 2004 this sequence version replaced NM_020158.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF281134.1, BC107696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGG AGACGCATAC TGACGCCAAA ATCCGTGCTG AAAATGGAAC AGGGTCCAGC 
CCTCGGGGTC CTGGCTGCAG CCTCCGGCAC TTTGCCTGCG AACAGAACCT GCTGTCGCGG 
CCAGATGGCT CTGCTTCCTT CCTGCAAGGT GACACCTCTG TCCTGGCGGG TGTGTACGGG 
CCGGCCGAGG TGAAGGTCAG CAAAGAGATT TTCAACAAGG CCACACTCGA AGTGATCCTG 
AGGCCGAAGA TTGGGCTGCC TGGTGTTGCA GAGAAGAGCC GGGAGCGGCT GATCAGGAAC 
ACGTGCGAGG CGGTGGTGCT GGGCACGTTG CACCCCCGCA CCTCCATCAC CGTGGTGCTG 
CAGGTTGTCA GCGATGCCGG CTCTCTCCTG GCCTGTTGTC TGAATGCCGC CTGCATGGCA 
TTGGTGGATG CAGGTGTGCC CATGCGGGCT CTCTTCTGTG GGGTCGCCTG CGCCCTGGAC 
TCTGATGGGA CCCTCGTGCT GGATCCTACA TCCAAGCAAG AAAAGGAGGC CCGGGCAGTC 
CTGACCTTTG CCCTGGACAG CGTGGAACGG AAGCTGCTGA TGTCCAGCAC CAAGGGGCTC 
TACTCAGACA CTGAGCTCCA GCAGTGCCTG GCTGCGGCCC AGGCCGCTTC GCAACACGTC 
TTCCGTTTCT ACCGGGAATC GCTGCAGAGG CGTTACTCCA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064543.3
CDS	24..731
Translation	MEEETHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS

Target ORF information:

RefSeq Version	NM_020158.3
Organism	Homo sapiens(human)
Definition	Homo sapiens exosome component 5 (EXOSC5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020158.3

ORF Insert Sequence:

ATGGAGGAGG AGACGCATAC TGACGCCAAA ATCCGTGCTG AAAATGGAAC AGGGTCCAGC 
CCTCGGGGTC CTGGCTGCAG CCTCCGGCAC TTTGCCTGCG AACAGAACCT GCTGTCGCGG 
CCAGATGGCT CTGCTTCCTT CCTGCAAGGT GACACCTCTG TCCTGGCGGG TGTGTACGGG 
CCGGCCGAGG TGAAGGTCAG CAAAGAGATT TTCAACAAGG CCACACTCGA AGTGATCCTG 
AGGCCGAAGA TTGGGCTGCC TGGTGTTGCA GAGAAGAGCC GGGAGCGGCT GATCAGGAAC 
ACGTGCGAGG CGGTGGTGCT GGGCACGTTG CACCCCCGCA CCTCCATCAC CGTGGTGCTG 
CAGGTTGTCA GCGATGCCGG CTCTCTCCTG GCCTGTTGTC TGAATGCCGC CTGCATGGCA 
TTGGTGGATG CAGGTGTGCC CATGCGGGCT CTCTTCTGTG GGGTCGCCTG CGCCCTGGAC 
TCTGATGGGA CCCTCGTGCT GGATCCTACA TCCAAGCAAG AAAAGGAGGC CCGGGCAGTC 
CTGACCTTTG CCCTGGACAG CGTGGAACGG AAGCTGCTGA TGTCCAGCAC CAAGGGGCTC 
TACTCAGACA CTGAGCTCCA GCAGTGCCTG GCTGCGGCCC AGGCCGCTTC GCAACACGTC 
TTCCGTTTCT ACCGGGAATC GCTGCAGAGG CGTTACTCCA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA.
Nature511(7510)435-9(2014 Jul)
Wasmuth EV,Januszyk K,Lima CD

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EXOSC5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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