PSMB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMB2
Entrez Gene ID	5690
Full Name	proteasome subunit beta 2
Synonyms	HC7-I
General protein information	Preferred Names proteasome subunit beta 2 Names proteasome subunit beta type-2 macropain subunit C7-I multicatalytic endopeptidase complex subunit C7-1 multicatalytic endopeptidase complex subunit C7-I proteasome (prosome, macropain) subunit, beta type, 2 proteasome beta 2 subunit proteasome component C7-I proteasome subunit, beta type, 2 testicular tissue protein Li 152
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.3
Summary	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010].
Disorder MIM:	602175

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199780.1	NP_001186709.1	proteasome subunit beta type-2 isoform 3
NM_001199779.2	NP_001186708.1	proteasome subunit beta type-2 isoform 2
NM_001199779.1	NP_001186708.1	proteasome subunit beta type-2 isoform 2
NM_002794.4	NP_002785.1	proteasome subunit beta type-2 isoform 1
NM_002794.5	NP_002785.1	proteasome subunit beta type-2 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSMB2	NP_002785.1
Pan troglodytes (chimpanzee)	PSMB2	NP_001233318.1
Bos taurus (cattle)	PSMB2	NP_001015615.1
Drosophila melanogaster (fruit fly)	CG17331	NP_001260511.1
Caenorhabditis elegans (roundworm)	pbs-4	NP_491261.1
Kluyveromyces lactis	KLLA0B11660g	XP_452049.1
Schizosaccharomyces pombe (fission yeast)	SPAC31A2.04c	NP_592916.1
Neurospora crassa	NCU01368	XP_960720.2
Arabidopsis thaliana (thale cress)	PBD2	NP_001190731.1
Xenopus tropicalis (tropical clawed frog)	psmb2	NP_001011057.1
Canis lupus familiaris (dog)	PSMB2	XP_532564.3
Mus musculus (house mouse)	Psmb2	NP_036100.3
Arabidopsis thaliana (thale cress)	PBD1	NP_188902.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008837	XP_319581.3
Saccharomyces cerevisiae (baker's yeast)	PRE1	NP_010928.1
Eremothecium gossypii	AGOS_AAR019W	NP_982560.1
Oryza sativa (rice)	Os03g0695600	NP_001050976.1
	PSMB2	XP_001109596.1
Rattus norvegicus (Norway rat)	Psmb2	NP_058980.1
Gallus gallus (chicken)	PSMB2	XP_417777.2
Danio rerio (zebrafish)	psmb2	NP_001002609.1
Magnaporthe oryzae (rice blast fungus)	MGG_07031	XP_003715213.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Crystal structure of the human 20S proteasome in complex with carfilzomib.
Harshbarger W, Miller C, Diedrich C, Sacchettini J
Structure (London, England : 1993)23(2)418-24(2015 Feb)

The over-expression of the ?2 catalytic subunit of the proteasome decreases homologous recombination and impairs DNA double-strand break repair in human cells.
Collavoli A, Comelli L, Cervelli T, Galli A
Journal of biomedicine & biotechnology2011757960(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results showed that the overexpression of beta2 subunit decreased homologous recombination in human cells without altering the cell proteasome activity and the Rad51p level.
Title: The over-expression of the ?2 catalytic subunit of the proteasome decreases homologous recombination and impairs DNA double-strand break repair in human cells.

This subunit propeptides influence cooperative proteasome assembly.
Title: Beta 2 subunit propeptides influence cooperative proteasome assembly.

The following PSMB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11123	NM_002794.5 Latest version!	Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08758	NM_001199780.1 Latest version!	Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu12362	NM_001199779.2 Latest version!	Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu12362	NM_001199779.1	Homo sapiens proteasome subunit beta 2 (PSMB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu11123	NM_002794.4	Homo sapiens proteasome subunit beta 2 (PSMB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMB2 ( NM_002794.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002785.1 Homo sapiens proteasome subunit beta 2 (PSMB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11123
Clone ID Related Accession (Same CDS sequence)	NM_002794.4 , NM_002794.5
Accession Version	NM_002794.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354864.16, BC101836.1, AL157951.5 and BM664618.1. On Dec 17, 2010 this sequence version replaced NM_002794.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.48160.1, SRR1660805.44471.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTACC TCATCGGTAT CCAAGGCCCC GACTATGTTC TTGTCGCCTC CGACCGGGTG 
GCCGCCAGCA ATATTGTCCA GATGAAGGAC GATCATGACA AGATGTTTAA GATGAGTGAA 
AAGATATTAC TCCTGTGTGT TGGAGAGGCT GGAGACACTG TACAGTTTGC AGAATATATT 
CAGAAAAACG TGCAACTTTA TAAGATGCGA AATGGATATG AATTGTCTCC CACGGCAGCA 
GCTAACTTCA CACGCCGAAA CCTGGCTGAC TGTCTTCGGA GTCGGACCCC ATATCATGTG 
AACCTCCTCC TGGCTGGCTA TGATGAGCAT GAAGGGCCAG CGCTGTATTA CATGGACTAC 
CTGGCAGCCT TGGCCAAGGC CCCTTTTGCA GCCCACGGCT ATGGTGCCTT CCTGACTCTC 
AGTATCCTCG ACCGATACTA CACACCGACT ATCTCACGTG AGAGGGCAGT GGAACTCCTT 
AGGAAATGTC TGGAGGAGCT CCAGAAACGC TTCATCCTGA ATCTGCCAAC CTTCAGTGTT 
CGAATCATTG ACAAAAATGG CATCCATGAC CTGGATAACA TTTCCTTCCC CAAACAGGGC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002785.1
CDS	413..1018
Translation	MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS

Target ORF information:

RefSeq Version	NM_002794.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome subunit beta 2 (PSMB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002794.4

ORF Insert Sequence:

ATGGAGTACC TCATCGGTAT CCAAGGCCCC GACTATGTTC TTGTCGCCTC CGACCGGGTG 
GCCGCCAGCA ATATTGTCCA GATGAAGGAC GATCATGACA AGATGTTTAA GATGAGTGAA 
AAGATATTAC TCCTGTGTGT TGGAGAGGCT GGAGACACTG TACAGTTTGC AGAATATATT 
CAGAAAAACG TGCAACTTTA TAAGATGCGA AATGGATATG AATTGTCTCC CACGGCAGCA 
GCTAACTTCA CACGCCGAAA CCTGGCTGAC TGTCTTCGGA GTCGGACCCC ATATCATGTG 
AACCTCCTCC TGGCTGGCTA TGATGAGCAT GAAGGGCCAG CGCTGTATTA CATGGACTAC 
CTGGCAGCCT TGGCCAAGGC CCCTTTTGCA GCCCACGGCT ATGGTGCCTT CCTGACTCTC 
AGTATCCTCG ACCGATACTA CACACCGACT ATCTCACGTG AGAGGGCAGT GGAACTCCTT 
AGGAAATGTC TGGAGGAGCT CCAGAAACGC TTCATCCTGA ATCTGCCAAC CTTCAGTGTT 
CGAATCATTG ACAAAAATGG CATCCATGAC CTGGATAACA TTTCCTTCCC CAAACAGGGC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB2 ( NM_002794.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002785.1 Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11123
Clone ID Related Accession (Same CDS sequence)	NM_002794.4 , NM_002794.5
Accession Version	NM_002794.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354864.16, BC101836.1, AL157951.5 and BM664618.1. On Nov 22, 2018 this sequence version replaced NM_002794.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.251306.1, SRR1803617.48160.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373237.4/ ENSP00000362334.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGTACC TCATCGGTAT CCAAGGCCCC GACTATGTTC TTGTCGCCTC CGACCGGGTG 
GCCGCCAGCA ATATTGTCCA GATGAAGGAC GATCATGACA AGATGTTTAA GATGAGTGAA 
AAGATATTAC TCCTGTGTGT TGGAGAGGCT GGAGACACTG TACAGTTTGC AGAATATATT 
CAGAAAAACG TGCAACTTTA TAAGATGCGA AATGGATATG AATTGTCTCC CACGGCAGCA 
GCTAACTTCA CACGCCGAAA CCTGGCTGAC TGTCTTCGGA GTCGGACCCC ATATCATGTG 
AACCTCCTCC TGGCTGGCTA TGATGAGCAT GAAGGGCCAG CGCTGTATTA CATGGACTAC 
CTGGCAGCCT TGGCCAAGGC CCCTTTTGCA GCCCACGGCT ATGGTGCCTT CCTGACTCTC 
AGTATCCTCG ACCGATACTA CACACCGACT ATCTCACGTG AGAGGGCAGT GGAACTCCTT 
AGGAAATGTC TGGAGGAGCT CCAGAAACGC TTCATCCTGA ATCTGCCAAC CTTCAGTGTT 
CGAATCATTG ACAAAAATGG CATCCATGAC CTGGATAACA TTTCCTTCCC CAAACAGGGC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002785.1
CDS	95..700
Translation	MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS

Target ORF information:

RefSeq Version	NM_002794.5
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002794.5

ORF Insert Sequence:

ATGGAGTACC TCATCGGTAT CCAAGGCCCC GACTATGTTC TTGTCGCCTC CGACCGGGTG 
GCCGCCAGCA ATATTGTCCA GATGAAGGAC GATCATGACA AGATGTTTAA GATGAGTGAA 
AAGATATTAC TCCTGTGTGT TGGAGAGGCT GGAGACACTG TACAGTTTGC AGAATATATT 
CAGAAAAACG TGCAACTTTA TAAGATGCGA AATGGATATG AATTGTCTCC CACGGCAGCA 
GCTAACTTCA CACGCCGAAA CCTGGCTGAC TGTCTTCGGA GTCGGACCCC ATATCATGTG 
AACCTCCTCC TGGCTGGCTA TGATGAGCAT GAAGGGCCAG CGCTGTATTA CATGGACTAC 
CTGGCAGCCT TGGCCAAGGC CCCTTTTGCA GCCCACGGCT ATGGTGCCTT CCTGACTCTC 
AGTATCCTCG ACCGATACTA CACACCGACT ATCTCACGTG AGAGGGCAGT GGAACTCCTT 
AGGAAATGTC TGGAGGAGCT CCAGAAACGC TTCATCCTGA ATCTGCCAAC CTTCAGTGTT 
CGAATCATTG ACAAAAATGG CATCCATGAC CTGGATAACA TTTCCTTCCC CAAACAGGGC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB2 ( NM_001199780.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186709.1 Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu08758
Clone ID Related Accession (Same CDS sequence)	NM_001199780.1
Accession Version	NM_001199780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310023.1, AL157951.5 and BM664618.1. Transcript Variant: This variant (3) lacks two exons from the 5' end and has an alternate 5' exon, as compared to variant 1. The resulting isoform (3) is shorter at the N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310023.1, DA917638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

ATGGACTACC TGGCAGCCTT GGCCAAGGCC CCTTTTGCAG CCCACGGCTA TGGTGCCTTC 
CTGACTCTCA GTATCCTCGA CCGATACTAC ACACCGACTA TCTCACGTGA GAGGGCAGTG 
GAACTCCTTA GGAAATGTCT GGAGGAGCTC CAGAAACGCT TCATCCTGAA TCTGCCAACC 
TTCAGTGTTC GAATCATTGA CAAAAATGGC ATCCATGACC TGGATAACAT TTCCTTCCCC 
AAACAGGGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186709.1
CDS	240..494
Translation	MDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS

Target ORF information:

RefSeq Version	NM_001199780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199780.1

ORF Insert Sequence:

ATGGACTACC TGGCAGCCTT GGCCAAGGCC CCTTTTGCAG CCCACGGCTA TGGTGCCTTC 
CTGACTCTCA GTATCCTCGA CCGATACTAC ACACCGACTA TCTCACGTGA GAGGGCAGTG 
GAACTCCTTA GGAAATGTCT GGAGGAGCTC CAGAAACGCT TCATCCTGAA TCTGCCAACC 
TTCAGTGTTC GAATCATTGA CAAAAATGGC ATCCATGACC TGGATAACAT TTCCTTCCCC 
AAACAGGGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB2 ( NM_001199779.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186708.1 Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12362
Clone ID Related Accession (Same CDS sequence)	NM_001199779.2 , NM_001199779.1
Accession Version	NM_001199779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296956.1, AL157951.5 and BM664618.1. On Jun 1, 2019 this sequence version replaced NM_001199779.1. Transcript Variant: This variant (2) lacks an in-frame segment in the 5' region, as compared to variant 1. The resulting isoform (2) lacks an internal segment in the N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296956.1, SRR1803612.33958.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTACC TCATCGATCA TGACAAGATG TTTAAGATGA GTGAAAAGAT ATTACTCCTG 
TGTGTTGGAG AGGCTGGAGA CACTGTACAG TTTGCAGAAT ATATTCAGAA AAACGTGCAA 
CTTTATAAGA TGCGAAATGG ATATGAATTG TCTCCCACGG CAGCAGCTAA CTTCACACGC 
CGAAACCTGG CTGACTGTCT TCGGAGTCGG ACCCCATATC ATGTGAACCT CCTCCTGGCT 
GGCTATGATG AGCATGAAGG GCCAGCGCTG TATTACATGG ACTACCTGGC AGCCTTGGCC 
AAGGCCCCTT TTGCAGCCCA CGGCTATGGT GCCTTCCTGA CTCTCAGTAT CCTCGACCGA 
TACTACACAC CGACTATCTC ACGTGAGAGG GCAGTGGAAC TCCTTAGGAA ATGTCTGGAG 
GAGCTCCAGA AACGCTTCAT CCTGAATCTG CCAACCTTCA GTGTTCGAAT CATTGACAAA 
AATGGCATCC ATGACCTGGA TAACATTTCC TTCCCCAAAC AGGGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186708.1
CDS	95..625
Translation	MEYLIDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS

Target ORF information:

RefSeq Version	NM_001199779.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 20S subunit beta 2 (PSMB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199779.2

ORF Insert Sequence:

ATGGAGTACC TCATCGATCA TGACAAGATG TTTAAGATGA GTGAAAAGAT ATTACTCCTG 
TGTGTTGGAG AGGCTGGAGA CACTGTACAG TTTGCAGAAT ATATTCAGAA AAACGTGCAA 
CTTTATAAGA TGCGAAATGG ATATGAATTG TCTCCCACGG CAGCAGCTAA CTTCACACGC 
CGAAACCTGG CTGACTGTCT TCGGAGTCGG ACCCCATATC ATGTGAACCT CCTCCTGGCT 
GGCTATGATG AGCATGAAGG GCCAGCGCTG TATTACATGG ACTACCTGGC AGCCTTGGCC 
AAGGCCCCTT TTGCAGCCCA CGGCTATGGT GCCTTCCTGA CTCTCAGTAT CCTCGACCGA 
TACTACACAC CGACTATCTC ACGTGAGAGG GCAGTGGAAC TCCTTAGGAA ATGTCTGGAG 
GAGCTCCAGA AACGCTTCAT CCTGAATCTG CCAACCTTCA GTGTTCGAAT CATTGACAAA 
AATGGCATCC ATGACCTGGA TAACATTTCC TTCCCCAAAC AGGGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB2 ( NM_001199779.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186708.1 Homo sapiens proteasome subunit beta 2 (PSMB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12362
Clone ID Related Accession (Same CDS sequence)	NM_001199779.2 , NM_001199779.1
Accession Version	NM_001199779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM849239.1, AK296956.1, AL157951.5 and BM664618.1. Transcript Variant: This variant (2) lacks an in-frame segment in the 5' region, as compared to variant 1. The resulting isoform (2) lacks an internal segment in the N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.361564.1, SRR1660803.92326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTACC TCATCGATCA TGACAAGATG TTTAAGATGA GTGAAAAGAT ATTACTCCTG 
TGTGTTGGAG AGGCTGGAGA CACTGTACAG TTTGCAGAAT ATATTCAGAA AAACGTGCAA 
CTTTATAAGA TGCGAAATGG ATATGAATTG TCTCCCACGG CAGCAGCTAA CTTCACACGC 
CGAAACCTGG CTGACTGTCT TCGGAGTCGG ACCCCATATC ATGTGAACCT CCTCCTGGCT 
GGCTATGATG AGCATGAAGG GCCAGCGCTG TATTACATGG ACTACCTGGC AGCCTTGGCC 
AAGGCCCCTT TTGCAGCCCA CGGCTATGGT GCCTTCCTGA CTCTCAGTAT CCTCGACCGA 
TACTACACAC CGACTATCTC ACGTGAGAGG GCAGTGGAAC TCCTTAGGAA ATGTCTGGAG 
GAGCTCCAGA AACGCTTCAT CCTGAATCTG CCAACCTTCA GTGTTCGAAT CATTGACAAA 
AATGGCATCC ATGACCTGGA TAACATTTCC TTCCCCAAAC AGGGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186708.1
CDS	120..650
Translation	MEYLIDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS

Target ORF information:

RefSeq Version	NM_001199779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome subunit beta 2 (PSMB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199779.1

ORF Insert Sequence:

ATGGAGTACC TCATCGATCA TGACAAGATG TTTAAGATGA GTGAAAAGAT ATTACTCCTG 
TGTGTTGGAG AGGCTGGAGA CACTGTACAG TTTGCAGAAT ATATTCAGAA AAACGTGCAA 
CTTTATAAGA TGCGAAATGG ATATGAATTG TCTCCCACGG CAGCAGCTAA CTTCACACGC 
CGAAACCTGG CTGACTGTCT TCGGAGTCGG ACCCCATATC ATGTGAACCT CCTCCTGGCT 
GGCTATGATG AGCATGAAGG GCCAGCGCTG TATTACATGG ACTACCTGGC AGCCTTGGCC 
AAGGCCCCTT TTGCAGCCCA CGGCTATGGT GCCTTCCTGA CTCTCAGTAT CCTCGACCGA 
TACTACACAC CGACTATCTC ACGTGAGAGG GCAGTGGAAC TCCTTAGGAA ATGTCTGGAG 
GAGCTCCAGA AACGCTTCAT CCTGAATCTG CCAACCTTCA GTGTTCGAAT CATTGACAAA 
AATGGCATCC ATGACCTGGA TAACATTTCC TTCCCCAAAC AGGGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Crystal structure of the human 20S proteasome in complex with carfilzomib.
Structure (London, England : 1993)23(2)418-24(2015 Feb)
Harshbarger W,Miller C,Diedrich C,Sacchettini J

The over-expression of the ?2 catalytic subunit of the proteasome decreases homologous recombination and impairs DNA double-strand break repair in human cells.
Journal of biomedicine & biotechnology2011757960(2011)
Collavoli A,Comelli L,Cervelli T,Galli A

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PSMB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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