PRRG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRRG1
Entrez Gene ID	5638
Full Name	proline rich and Gla domain 1
Synonyms	PRGP1
General protein information	Preferred Names proline rich and Gla domain 1 Names transmembrane gamma-carboxyglutamic acid protein 1 proline rich Gla (G-carboxyglutamic acid) 1 proline-rich Gla (G-carboxyglutamic acid) polypeptide 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp21.1
Summary	This gene encodes a vitamin K-dependent, gamma-carboxyglutamic acid (Gla)-containing, single-pass transmembrane protein. This protein contains a Gla domain at the N-terminus, preceded by a propeptide sequence required for post-translational gamma-carboxylation of specific glutamic acid residues by a vitamin K-dependent gamma-carboxylase. The C-terminus is proline-rich containing PPXY and PXXP motifs found in a variety of signaling and cytoskeletal proteins. This gene is highly expressed in the spinal cord. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2010].
Disorder MIM:	300935

mRNA and Protein(s)

mRNA	Protein	Name
NM_001173486.1	NP_001166957.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 2 precursor
NM_000950.2	NP_000941.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
NM_001142395.1	NP_001135867.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
NM_001173490.1	NP_001166961.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
NM_001173486.2	NP_001166957.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 2 precursor
NM_000950.3	NP_000941.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
NM_001142395.2	NP_001135867.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
NM_001173489.1	NP_001166960.1	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRRG1	NP_001166961.1
Danio rerio (zebrafish)	zgc:103657	NP_001005948.1
Xenopus tropicalis (tropical clawed frog)	prrg1	NP_001017345.1
	PRRG1	XP_001085543.2
Mus musculus (house mouse)	Prrg1	NP_081598.1
Gallus gallus (chicken)	PRRG1	NP_001264665.1
Canis lupus familiaris (dog)	LOC491821	XP_548941.3
Bos taurus (cattle)	PRRG1	NP_001099110.1
Rattus norvegicus (Norway rat)	Prrg1	NP_001178817.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags.
Wang AG, Yoon SY, Oh JH, Jeon YJ, Kim M, Kim JM, Byun SS, Yang JO, Kim JH, Kim DG, Yeom YI, Yoo HS, Kim YS, Kim NS
Biochemical and biophysical research communications345(3)1022-32(2006 Jul)

The DNA sequence of the human X chromosome.
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, Frankish A, Lovell FL, Howe KL, Ashurst JL, Fulton RS, Sudbrak R, Wen G, Jones MC, Hurles ME, Andrews TD, Scott CE, Searle S, Ramser J, Whittaker A, Deadman R, Carter NP, Hunt SE, Chen R, Cree A, Gunaratne P, Havlak P, Hodgson A, Metzker ML, Richards S, Scott G, Steffen D, Sodergren E, Wheeler DA, Worley KC, Ainscough R, Ambrose KD, Ansari-Lari MA, Aradhya S, Ashwell RI, Babbage AK, Bagguley CL, Ballabio A, Banerjee R, Barker GE, Barlow KF, Barrett IP, Bates KN, Beare DM, Beasley H, Beasley O, Beck A, Bethel G, Blechschmidt K, Brady N, Bray-Allen S, Bridgeman AM, Brown AJ, Brown MJ, Bonnin D, Bruford EA, Buhay C, Burch P, Burford D, Burgess J, Burrill W, Burton J, Bye JM, Carder C, Carrel L, Chako J, Chapman JC, Chavez D, Chen E, Chen G, Chen Y, Chen Z, Chinault C, Ciccodicola A, Clark SY, Clarke G, Clee CM, Clegg S, Clerc-Blankenburg K, Clifford K, Cobley V, Cole CG, Conquer JS, Corby N, Connor RE, David R, Davies J, Davis C, Davis J, Delgado O, Deshazo D, Dhami P, Ding Y, Dinh H, Dodsworth S, Draper H, Dugan-Rocha S, Dunham A, Dunn M, Durbin KJ, Dutta I, Eades T, Ellwood M, Emery-Cohen A, Errington H, Evans KL, Faulkner L, Francis F, Frankland J, Fraser AE, Galgoczy P, Gilbert J, Gill R, Gl?ckner G, Gregory SG, Gribble S, Griffiths C, Grocock R, Gu Y, Gwilliam R, Hamilton C, Hart EA, Hawes A, Heath PD, Heitmann K, Hennig S, Hernandez J, Hinzmann B, Ho S, Hoffs M, Howden PJ, Huckle EJ, Hume J, Hunt PJ, Hunt AR, Isherwood J, Jacob L, Johnson D, Jones S, de Jong PJ, Joseph SS, Keenan S, Kelly S, Kershaw JK, Khan Z, Kioschis P, Klages S, Knights AJ, Kosiura A, Kovar-Smith C, Laird GK, Langford C, Lawlor S, Leversha M, Lewis L, Liu W, Lloyd C, Lloyd DM, Loulseged H, Loveland JE, Lovell JD, Lozado R, Lu J, Lyne R, Ma J, Maheshwari M, Matthews LH, McDowall J, McLaren S, McMurray A, Meidl P, Meitinger T, Milne S, Miner G, Mistry SL, Morgan M, Morris S, M?ller I, Mullikin JC, Nguyen N, Nordsiek G, Nyakatura G, O'Dell CN, Okwuonu G, Palmer S, Pandian R, Parker D, Parrish J, Pasternak S, Patel D, Pearce AV, Pearson DM, Pelan SE, Perez L, Porter KM, Ramsey Y, Reichwald K, Rhodes S, Ridler KA, Schlessinger D, Schueler MG, Sehra HK, Shaw-Smith C, Shen H, Sheridan EM, Shownkeen R, Skuce CD, Smith ML, Sotheran EC, Steingruber HE, Steward CA, Storey R, Swann RM, Swarbreck D, Tabor PE, Taudien S, Taylor T, Teague B, Thomas K, Thorpe A, Timms K, Tracey A, Trevanion S, Tromans AC, d'Urso M, Verduzco D, Villasana D, Waldron L, Wall M, Wang Q, Warren J, Warry GL, Wei X, West A, Whitehead SL, Whiteley MN, Wilkinson JE, Willey DL, Williams G, Williams L, Williamson A, Williamson H, Wilming L, Woodmansey RL, Wray PW, Yen J, Zhang J, Zhou J, Zoghbi H, Zorilla S, Buck D, Reinhardt R, Poustka A, Rosenthal A, Lehrach H, Meindl A, Minx PJ, Hillier LW, Willard HF, Wilson RK, Waterston RH, Rice CM, Vaudin M, Coulson A, Nelson DL, Weinstock G, Sulston JE, Durbin R, Hubbard T, Gibbs RA, Beck S, Rogers J, Bentley DR
Nature434(7031)325-37(2005 Mar)

Primary structure and tissue distribution of two novel proline-rich gamma-carboxyglutamic acid proteins.
Kulman JD, Harris JE, Haldeman BA, Davie EW
Proceedings of the National Academy of Sciences of the United States of America94(17)9058-62(1997 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRRG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRRG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04605	NM_001142395.2 Latest version!	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04605	NM_001173489.1 Latest version!	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04605	NM_000950.3 Latest version!	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04605	NM_001173490.1 Latest version!	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu11920	NM_001173486.2 Latest version!	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu11920	NM_001173486.1	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu04605	NM_000950.2	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04605	NM_001142395.1	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRRG1 ( NM_000950.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000941.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04605
Clone ID Related Accession (Same CDS sequence)	NM_000950.2 , NM_001142395.1 , NM_001173490.1 , NM_000950.3 , NM_001142395.2 , NM_001173489.1
Accession Version	NM_000950.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB286340.1, DA411839.1, AF009242.1 and BC030786.2. This sequence is a reference standard in the RefSeqGene project. On Dec 2, 2008 this sequence version replaced NM_000950.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1-4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.87339.1, SRR1803611.121966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000941.1
CDS	254..910
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK

Target ORF information:

RefSeq Version	NM_000950.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000950.2

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_001142395.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135867.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04605
Clone ID Related Accession (Same CDS sequence)	NM_000950.2 , NM_001142395.1 , NM_001173490.1 , NM_000950.3 , NM_001142395.2 , NM_001173489.1
Accession Version	NM_001142395.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB286340.1, BC060833.1 and BC030786.2. Transcript Variant: This variant (2) is missing a 5' non-coding exon compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC030786.2, SRR1803617.196195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135867.1
CDS	163..819
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK

Target ORF information:

RefSeq Version	NM_001142395.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142395.1

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_001173490.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166961.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu04605
Clone ID Related Accession (Same CDS sequence)	NM_000950.2 , NM_001142395.1 , NM_001173490.1 , NM_000950.3 , NM_001142395.2 , NM_001173489.1
Accession Version	NM_001173490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA417581.1, AF009242.1, BX097465.1 and CB134819.1. Transcript Variant: This variant (4) uses an alternative donor splice site at the 5' terminal non-coding exon, and is missing another 5' non-coding exon compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: DA417581.1, SRR1803617.39183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166961.1
CDS	182..838
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK

Target ORF information:

RefSeq Version	NM_001173490.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173490.1

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_000950.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000941.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04605
Clone ID Related Accession (Same CDS sequence)	NM_000950.2 , NM_001142395.1 , NM_001173490.1 , NM_000950.3 , NM_001142395.2 , NM_001173489.1
Accession Version	NM_000950.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA411839.1, AF009242.1 and BC030786.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000950.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1-4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.87339.1, SRR1803611.121966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000941.1
CDS	165..821
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK

Target ORF information:

RefSeq Version	NM_000950.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000950.3

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_001142395.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135867.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04605
Clone ID Related Accession (Same CDS sequence)	NM_000950.2 , NM_001142395.1 , NM_001173490.1 , NM_000950.3 , NM_001142395.2 , NM_001173489.1
Accession Version	NM_001142395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060833.1 and BC030786.2. On Jun 2, 2019 this sequence version replaced NM_001142395.1. Transcript Variant: This variant (2) is missing a 5' non-coding exon compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.81417.1, SRR1803613.100004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135867.1
CDS	74..730
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK

Target ORF information:

RefSeq Version	NM_001142395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142395.2

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_001173489.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166960.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu04605
Clone ID Related Accession (Same CDS sequence)	NM_000950.2 , NM_001142395.1 , NM_001173490.1 , NM_000950.3 , NM_001142395.2 , NM_001173489.1
Accession Version	NM_001173489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA417581.1, DA245329.1, AF009242.1, BX097465.1 and CB134819.1. Transcript Variant: This variant (3) uses an alternative donor splice site at the 5' terminal non-coding exon compared to variant 1. Variants 1-4 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: DA245329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145774, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166960.1
CDS	273..929
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTKEFWSTYTKAQQGESNRGSDWFQFYLTFPLIFGLFIILLVIFLIWRCFLRNKTRRQTVTEGHIPFPQHLNIITPPPPPDEVFDSSGLSPGFLGYVVGRSDSVSTRLSNCDPPPTYEEATGQVNLQRSETEPHLDPPPEYEDIVNSNSASAIPMVPVVTTIK

Target ORF information:

RefSeq Version	NM_001173489.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173489.1

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TAAGGAGTTT 
TGGAGCACCT ACACAAAAGC GCAACAAGGG GAGAGTAACC GAGGAAGTGA CTGGTTTCAG 
TTTTACCTTA CCTTTCCGTT AATCTTTGGC CTCTTCATTA TCCTCCTTGT CATTTTCCTA 
ATCTGGAGAT GCTTCCTAAG AAACAAAACT CGTAGACAGA CAGTGACTGA AGGCCACATT 
CCTTTCCCTC AGCACCTTAA TATTATCACC CCACCCCCCC CACCAGATGA AGTGTTTGAC 
AGCAGTGGAT TGTCTCCAGG CTTTCTGGGA TATGTAGTTG GGCGCTCAGA TTCCGTCTCT 
ACTCGCCTGT CCAATTGTGA TCCCCCGCCA ACCTATGAGG AAGCCACTGG CCAAGTGAAC 
CTGCAGAGGA GTGAAACAGA ACCTCATTTA GACCCACCCC CAGAGTATGA GGACATAGTC 
AACTCCAACT CAGCCAGTGC CATTCCTATG GTGCCTGTGG TCACCACCAT CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_001173486.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166957.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu11920
Clone ID Related Accession (Same CDS sequence)	NM_001173486.1 , NM_001173486.2
Accession Version	NM_001173486.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB286340.1, BC060833.1, BC041591.1 and AC245096.1. Transcript Variant: This variant (5) is missing a 5' non-coding exon, and contains an alternate 3' terminal exon compared to variant 1. The latter causes a frame-shift and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. Isoform 2 retains a partial Gla domain at the N-terminus, however, this protein is predicted and lacks experimental evidence. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.222869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145313, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TGGTCTTGTT 
CTGTTGCCAA GGCTGGAGTG CAGCTGTGAA CATGGCTCAT TGCAGCCTCA ACTTCCTGTG 
CCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166957.1
CDS	163..411
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTGLVLLPRLECSCEHGSLQPQLPVPK

Target ORF information:

RefSeq Version	NM_001173486.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173486.1

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TGGTCTTGTT 
CTGTTGCCAA GGCTGGAGTG CAGCTGTGAA CATGGCTCAT TGCAGCCTCA ACTTCCTGTG 
CCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRG1 ( NM_001173486.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166957.1 Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu11920
Clone ID Related Accession (Same CDS sequence)	NM_001173486.1 , NM_001173486.2
Accession Version	NM_001173486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	transmembrane gamma-carboxyglutamic acid protein 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC060833.1, BC041591.1 and AC245096.1. On Jun 2, 2019 this sequence version replaced NM_001173486.1. Transcript Variant: This variant (5) is missing a 5' non-coding exon, and contains an alternate 3' terminal exon compared to variant 1. The latter causes a frame-shift and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. Isoform 2 retains a partial Gla domain at the N-terminus, however, this protein is predicted and lacks experimental evidence. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.222869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145313, SAMN03465410 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TGGTCTTGTT 
CTGTTGCCAA GGCTGGAGTG CAGCTGTGAA CATGGCTCAT TGCAGCCTCA ACTTCCTGTG 
CCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166957.1
CDS	74..322
Translation	MGRVFLTGEKANSILKRYPRANGFFEEIRQGNIERECKEEFCTFEEAREAFENNEKTGLVLLPRLECSCEHGSLQPQLPVPK

Target ORF information:

RefSeq Version	NM_001173486.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proline rich and Gla domain 1 (PRRG1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173486.2

ORF Insert Sequence:

ATGGGGAGGG TTTTCCTCAC GGGAGAAAAA GCCAATTCCA TATTAAAACG CTACCCAAGA 
GCTAATGGGT TTTTTGAAGA AATAAGACAG GGCAACATTG AGCGTGAGTG CAAAGAAGAA 
TTCTGTACAT TTGAAGAAGC AAGAGAAGCT TTTGAAAATA ATGAAAAAAC TGGTCTTGTT 
CTGTTGCCAA GGCTGGAGTG CAGCTGTGAA CATGGCTCAT TGCAGCCTCA ACTTCCTGTG 
CCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags.
Biochemical and biophysical research communications345(3)1022-32(2006 Jul)
Wang AG,Yoon SY,Oh JH,Jeon YJ,Kim M,Kim JM,Byun SS,Yang JO,Kim JH,Kim DG,Yeom YI,Yoo HS,Kim YS,Kim NS

The DNA sequence of the human X chromosome.
Nature434(7031)325-37(2005 Mar)
Ross MT,Grafham DV,Coffey AJ,Scherer S,McLay K,Muzny D,Platzer M,Howell GR,Burrows C,Bird CP,Frankish A,Lovell FL,Howe KL,Ashurst JL,Fulton RS,Sudbrak R,Wen G,Jones MC,Hurles ME,Andrews TD,Scott CE,Searle S,Ramser J,Whittaker A,Deadman R,Carter NP,Hunt SE,Chen R,Cree A,Gunaratne P,Havlak P,Hodgson A,Metzker ML,Richards S,Scott G,Steffen D,Sodergren E,Wheeler DA,Worley KC,Ainscough R,Ambrose KD,Ansari-Lari MA,Aradhya S,Ashwell RI,Babbage AK,Bagguley CL,Ballabio A,Banerjee R,Barker GE,Barlow KF,Barrett IP,Bates KN,Beare DM,Beasley H,Beasley O,Beck A,Bethel G,Blechschmidt K,Brady N,Bray-Allen S,Bridgeman AM,Brown AJ,Brown MJ,Bonnin D,Bruford EA,Buhay C,Burch P,Burford D,Burgess J,Burrill W,Burton J,Bye JM,Carder C,Carrel L,Chako J,Chapman JC,Chavez D,Chen E,Chen G,Chen Y,Chen Z,Chinault C,Ciccodicola A,Clark SY,Clarke G,Clee CM,Clegg S,Clerc-Blankenburg K,Clifford K,Cobley V,Cole CG,Conquer JS,Corby N,Connor RE,David R,Davies J,Davis C,Davis J,Delgado O,Deshazo D,Dhami P,Ding Y,Dinh H,Dodsworth S,Draper H,Dugan-Rocha S,Dunham A,Dunn M,Durbin KJ,Dutta I,Eades T,Ellwood M,Emery-Cohen A,Errington H,Evans KL,Faulkner L,Francis F,Frankland J,Fraser AE,Galgoczy P,Gilbert J,Gill R,Gl?ckner G,Gregory SG,Gribble S,Griffiths C,Grocock R,Gu Y,Gwilliam R,Hamilton C,Hart EA,Hawes A,Heath PD,Heitmann K,Hennig S,Hernandez J,Hinzmann B,Ho S,Hoffs M,Howden PJ,Huckle EJ,Hume J,Hunt PJ,Hunt AR,Isherwood J,Jacob L,Johnson D,Jones S,de Jong PJ,Joseph SS,Keenan S,Kelly S,Kershaw JK,Khan Z,Kioschis P,Klages S,Knights AJ,Kosiura A,Kovar-Smith C,Laird GK,Langford C,Lawlor S,Leversha M,Lewis L,Liu W,Lloyd C,Lloyd DM,Loulseged H,Loveland JE,Lovell JD,Lozado R,Lu J,Lyne R,Ma J,Maheshwari M,Matthews LH,McDowall J,McLaren S,McMurray A,Meidl P,Meitinger T,Milne S,Miner G,Mistry SL,Morgan M,Morris S,M?ller I,Mullikin JC,Nguyen N,Nordsiek G,Nyakatura G,O'Dell CN,Okwuonu G,Palmer S,Pandian R,Parker D,Parrish J,Pasternak S,Patel D,Pearce AV,Pearson DM,Pelan SE,Perez L,Porter KM,Ramsey Y,Reichwald K,Rhodes S,Ridler KA,Schlessinger D,Schueler MG,Sehra HK,Shaw-Smith C,Shen H,Sheridan EM,Shownkeen R,Skuce CD,Smith ML,Sotheran EC,Steingruber HE,Steward CA,Storey R,Swann RM,Swarbreck D,Tabor PE,Taudien S,Taylor T,Teague B,Thomas K,Thorpe A,Timms K,Tracey A,Trevanion S,Tromans AC,d'Urso M,Verduzco D,Villasana D,Waldron L,Wall M,Wang Q,Warren J,Warry GL,Wei X,West A,Whitehead SL,Whiteley MN,Wilkinson JE,Willey DL,Williams G,Williams L,Williamson A,Williamson H,Wilming L,Woodmansey RL,Wray PW,Yen J,Zhang J,Zhou J,Zoghbi H,Zorilla S,Buck D,Reinhardt R,Poustka A,Rosenthal A,Lehrach H,Meindl A,Minx PJ,Hillier LW,Willard HF,Wilson RK,Waterston RH,Rice CM,Vaudin M,Coulson A,Nelson DL,Weinstock G,Sulston JE,Durbin R,Hubbard T,Gibbs RA,Beck S,Rogers J,Bentley DR

Primary structure and tissue distribution of two novel proline-rich gamma-carboxyglutamic acid proteins.
Proceedings of the National Academy of Sciences of the United States of America94(17)9058-62(1997 Aug)
Kulman JD,Harris JE,Haldeman BA,Davie EW

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PRRG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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