RNF20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF20
Entrez Gene ID	56254
Full Name	ring finger protein 20
Synonyms	BRE1,BRE1A,hBRE1
General protein information	Preferred Names ring finger protein 20 Names E3 ubiquitin-protein ligase BRE1A BRE1 E3 ubiquitin ligase homolog RING-type E3 ubiquitin transferase BRE1A homolog of S. cerevisiae BRE1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q31.1
Summary	The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012].
Disorder MIM:	607699

mRNA and Protein(s)

mRNA	Protein	Name
NM_019592.7	NP_062538.5	E3 ubiquitin-protein ligase BRE1A
NM_019592.6	NP_062538.5	E3 ubiquitin-protein ligase BRE1A
XM_011518862.1	XP_011517164.1	E3 ubiquitin-protein ligase BRE1A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RNF20	XP_005626937.1
Rattus norvegicus (Norway rat)	Rnf20	NP_001101399.2
Xenopus tropicalis (tropical clawed frog)	rnf20	XP_002941911.1
Mus musculus (house mouse)	Rnf20	NP_001156735.1
Gallus gallus (chicken)	RNF20	NP_001026605.1
Pan troglodytes (chimpanzee)	RNF20	XP_520159.2
	RNF20	XP_001110807.1
Bos taurus (cattle)	RNF20	NP_001075056.1
Danio rerio (zebrafish)	rnf20	NP_001243104.1
Homo sapiens (human)	RNF20	NP_062538.5

Gene Ontology
Bibliography

Related articles in PubMed

Autophagy-related protein ATG5 regulates histone H2B mono-ubiquitylation by translational control of RNF20.
Huang X, Yang L, Cai FF, Wang Y, Chen P, Mi J, Yu C, Lai J, Zhang X, Wei S, Cui W, Chen S
Journal of genetics and genomics = Yi chuan xue bao44(10)503-506(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Manipulation of key H2Bub1 E3 ubiquitin ligases, RNF20, RNF40 and BRCA1, in ovarian cancer cell line models modulated H2Bub1 levels, indicative of the role of these RING finger ligases in monoubiquitination of H2Bub1 in vitro
Title: The RING finger domain E3 ubiquitin ligases BRCA1 and the RNF20/RNF40 complex in global loss of the chromatin mark histone H2B monoubiquitination (H2Bub1) in cell line models and primary high-grade serous ovarian cancer.

The authors also show that the RING domains of RNF20 and RNF40 can form a stable heterodimer that is active.
Title: Structure and Function of the RING Domains of RNF20 and RNF40, Dimeric E3 Ligases that Monoubiquitylate Histone H2B.

RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response.
Title: RNF20 Links Histone H2B Ubiquitylation with Inflammation and Inflammation-Associated Cancer.

It was observed that RE-IIBP induces MEIS1-mediated apoptosis, which was dependent on H2BK120 ubiquitination by RNF20.
Title: RE-IIBP Methylates H3K79 and Induces MEIS1-mediated Apoptosis via H2BK120 Ubiquitination by RNF20.

Together, these results indicate that human adenovirus E1A uses hBre1 to recruit the hPaf1 complex in order to optimally activate viral early transcription by enhancing transcriptional elongation.
Title: Adenovirus E1A recruits the human Paf1 complex to enhance transcriptional elongation.

The following RNF20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29182	NM_019592.7 Latest version!	Homo sapiens ring finger protein 20 (RNF20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu29182	XM_011518862.1 Latest version!	Homo sapiens ring finger protein 20 (RNF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu29182	NM_019592.6	Homo sapiens ring finger protein 20 (RNF20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF20 ( NM_019592.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_062538.5 Homo sapiens ring finger protein 20 (RNF20), mRNA.

Price & Availability↑

CloneID	OHu29182
Clone ID Related Accession (Same CDS sequence)	NM_019592.7 , NM_019592.6 , XM_011518862.1
Accession Version	NM_019592.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF265230.1, BC110584.1 and AL832910.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_019592.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022532.1, AF265230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389120.8/ ENSP00000373772.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAACCTG GCACCTCCAT GCCTCCTGAG 
AAGAAGGCAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAGCT AGGAGGTGTC 
TCTTCAACGG AGGAACTAGA CATTAGAACA CTGCAAACCA AAAATCGCAA GCTGGCAGAA 
ATGTTGGATC AGCGGCAGGC CATTGAAGAT GAACTTCGTG AGCACATTGA AAAACTGGAA 
CGACGACAGG CCACTGATGA TGCCTCACTA TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGT TATGATCTGG AGCAGGGCTT GGGAGACCTA 
CTCACAGAAC GAAAAGCCCT TGTTGTGCCT GAACCAGAAC CAGACTCTGA TAGCAATCAG 
GAGCGTAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAGTCTCAGC TGCAGGAACG TGTGGAGTCT 
TCCCGCCGAG CCGTGTCCCA GATTGTGACT GTTTATGATA AATTGCAAGA AAAAGTGGAG 
CTCTTATCCC GGAAGCTAAA CAGTGGAGAT AATCTGATAG TGGAGGAAGC AGTGCAGGAG 
CTGAACTCTT TCCTCGCACA GGAGAATATG AGGCTACAGG AATTGACAGA TCTTCTTCAG 
GAAAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTGC AGAGTAAAGT GGAGACAGCC 
GAATCACGAG TGTCTGTCCT GGAGTCCATG ATTGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAGCG ACTCAACCGA CACTTAGCAG AAGTCCTAGA ACGGGTGAAT 
TCCAAAGGTT ATAAGGTGTA TGGAGCGGGG AGCAGTCTGT ATGGCGGCAC AATCACTATC 
AATGCTCGGA AGTTTGAGGA AATGAATGCA GAGCTTGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCTCT GTGAGCTGGA GAAACTTCGG CAAGACTTTG AGGAGGTCAC TACACAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGTGCA GTGGAGCAAG TCGTTAAGGA AACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTC TTGTATAATG AGAGCCTACA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACCAGAG GAACCCACCA GCACCAGGTT 
GAGCTTATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTAATTCAG 
CTAGAAGATA CATTGGCCCA GGTCCGCAAG GAGTATGAAA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCTATAAACA GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTGAGATA TAAGCGGAAA 
TTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGCCTGC GTAGTGGTAG TGCCCTCCTG 
CAGTCCCAGT CTAGTACTGA GGACCCGAAG GATGAGCCTG CGGAGCTAAA ACCAGATTCT 
GAGGACTTAT CCTCCCAGTC CTCAGCTTCA AAGGCATCTC AGGAGGATGC CAATGAAATC 
AAGTCTAAAC GGGATGAAGA AGAACGAGAA CGAGAAAGGA GGGAGAAGGA GAGGGAACGA 
GAAAGAGAAC GGGAGAAGGA GAAGGAGAGA GAACGAGAGA AGCAGAAGCT AAAAGAGTCA 
GAAAAAGAGA GAGATTCTGC TAAGGATAAA GAGAAAGGCA AACATGATGA TGGACGGAAA 
AAGGAAGCAG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA GGAGAGCCAA 
AAGGAGATGA AACTATTGCT GGATATGTAC CGTTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCT AAGGCAGAGT TGGAAGATCT AAGGCAAAGA 
CTCAAGGATC TGGAAGATAA AGAGAAGAAA GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT GGAGGAGCAG ATAGAATACC TACAGAAGAA GCTAGCCATG 
GCCAAGCAGG AAGAAGAAGC ACTCCTCTCT GAAATGGATG TCACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTGATG CAGCAATTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCAGAGCG TATCAAGTCC AATCAGATCC ATAAGTTGCT TAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA GGTGTTGACT CTGAAGACTC AGGTTGATGC CCAGCTACAG 
GTAGTAAGGA AACTGGAAGA GAAGGAGCAT CTGTTACAGA GCAACATTGG CACAGGGGAG 
AAAGAGCTGG GTCTTAGGAC CCAAGCCTTA GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAA CTGGAGTTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAACAGTGTT ACCAAAGAAA AGGACATGTT CAATTTCAAA 
CGAGCCCAGG AGGACATCTC TAGACTTCGC AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTACCCA AGTGTGATGA GATTCTGATG GAAGAGATTA AGGATTACAA GGCACGCTTG 
ACCTGTCCGT GCTGTAACAT GCGTAAAAAG GATGCTGTTC TTACTAAGTG TTTTCATGTC 
TTCTGCTTTG AGTGTGTGAA GACACGCTAT GACACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCTT TTGGTGCCAA TGATTTTCAT CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062538.5
CDS	89..3016
Translation	MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASKASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	NM_019592.7
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 20 (RNF20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019592.7

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAACCTG GCACCTCCAT GCCTCCTGAG 
AAGAAGGCAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAGCT AGGAGGTGTC 
TCTTCAACGG AGGAACTAGA CATTAGAACA CTGCAAACCA AAAATCGCAA GCTGGCAGAA 
ATGTTGGATC AGCGGCAGGC CATTGAAGAT GAACTTCGTG AGCACATTGA AAAACTGGAA 
CGACGACAGG CCACTGATGA TGCCTCACTA TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGT TATGATCTGG AGCAGGGCTT GGGAGACCTA 
CTCACAGAAC GAAAAGCCCT TGTTGTGCCT GAACCAGAAC CAGACTCTGA TAGCAATCAG 
GAGCGTAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAGTCTCAGC TGCAGGAACG TGTGGAGTCT 
TCCCGCCGAG CCGTGTCCCA GATTGTGACT GTTTATGATA AATTGCAAGA AAAAGTGGAG 
CTCTTATCCC GGAAGCTAAA CAGTGGAGAT AATCTGATAG TGGAGGAAGC AGTGCAGGAG 
CTGAACTCTT TCCTCGCACA GGAGAATATG AGGCTACAGG AATTGACAGA TCTTCTTCAG 
GAAAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTGC AGAGTAAAGT GGAGACAGCC 
GAATCACGAG TGTCTGTCCT GGAGTCCATG ATTGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAGCG ACTCAACCGA CACTTAGCAG AAGTCCTAGA ACGGGTGAAT 
TCCAAAGGTT ATAAGGTGTA TGGAGCGGGG AGCAGTCTGT ATGGCGGCAC AATCACTATC 
AATGCTCGGA AGTTTGAGGA AATGAATGCA GAGCTTGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCTCT GTGAGCTGGA GAAACTTCGG CAAGACTTTG AGGAGGTCAC TACACAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGTGCA GTGGAGCAAG TCGTTAAGGA AACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTC TTGTATAATG AGAGCCTACA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACCAGAG GAACCCACCA GCACCAGGTT 
GAGCTTATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTAATTCAG 
CTAGAAGATA CATTGGCCCA GGTCCGCAAG GAGTATGAAA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCTATAAACA GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTGAGATA TAAGCGGAAA 
TTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGCCTGC GTAGTGGTAG TGCCCTCCTG 
CAGTCCCAGT CTAGTACTGA GGACCCGAAG GATGAGCCTG CGGAGCTAAA ACCAGATTCT 
GAGGACTTAT CCTCCCAGTC CTCAGCTTCA AAGGCATCTC AGGAGGATGC CAATGAAATC 
AAGTCTAAAC GGGATGAAGA AGAACGAGAA CGAGAAAGGA GGGAGAAGGA GAGGGAACGA 
GAAAGAGAAC GGGAGAAGGA GAAGGAGAGA GAACGAGAGA AGCAGAAGCT AAAAGAGTCA 
GAAAAAGAGA GAGATTCTGC TAAGGATAAA GAGAAAGGCA AACATGATGA TGGACGGAAA 
AAGGAAGCAG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA GGAGAGCCAA 
AAGGAGATGA AACTATTGCT GGATATGTAC CGTTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCT AAGGCAGAGT TGGAAGATCT AAGGCAAAGA 
CTCAAGGATC TGGAAGATAA AGAGAAGAAA GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT GGAGGAGCAG ATAGAATACC TACAGAAGAA GCTAGCCATG 
GCCAAGCAGG AAGAAGAAGC ACTCCTCTCT GAAATGGATG TCACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTGATG CAGCAATTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCAGAGCG TATCAAGTCC AATCAGATCC ATAAGTTGCT TAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA GGTGTTGACT CTGAAGACTC AGGTTGATGC CCAGCTACAG 
GTAGTAAGGA AACTGGAAGA GAAGGAGCAT CTGTTACAGA GCAACATTGG CACAGGGGAG 
AAAGAGCTGG GTCTTAGGAC CCAAGCCTTA GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAA CTGGAGTTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAACAGTGTT ACCAAAGAAA AGGACATGTT CAATTTCAAA 
CGAGCCCAGG AGGACATCTC TAGACTTCGC AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTACCCA AGTGTGATGA GATTCTGATG GAAGAGATTA AGGATTACAA GGCACGCTTG 
ACCTGTCCGT GCTGTAACAT GCGTAAAAAG GATGCTGTTC TTACTAAGTG TTTTCATGTC 
TTCTGCTTTG AGTGTGTGAA GACACGCTAT GACACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCTT TTGGTGCCAA TGATTTTCAT CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( NM_019592.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_062538.5 Homo sapiens ring finger protein 20 (RNF20), mRNA.

Price & Availability↑

CloneID	OHu29182
Clone ID Related Accession (Same CDS sequence)	NM_019592.7 , NM_019592.6 , XM_011518862.1
Accession Version	NM_019592.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB273543.1, AF265230.1, BC110584.1 and AL832910.1. This sequence is a reference standard in the RefSeqGene project. On Jul 9, 2013 this sequence version replaced NM_019592.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022532.1, AF265230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAACCTG GCACCTCCAT GCCTCCTGAG 
AAGAAGGCAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAGCT AGGAGGTGTC 
TCTTCAACGG AGGAACTAGA CATTAGAACA CTGCAAACCA AAAATCGCAA GCTGGCAGAA 
ATGTTGGATC AGCGGCAGGC CATTGAAGAT GAACTTCGTG AGCACATTGA AAAACTGGAA 
CGACGACAGG CCACTGATGA TGCCTCACTA TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGT TATGATCTGG AGCAGGGCTT GGGAGACCTA 
CTCACAGAAC GAAAAGCCCT TGTTGTGCCT GAACCAGAAC CAGACTCTGA TAGCAATCAG 
GAGCGTAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAGTCTCAGC TGCAGGAACG TGTGGAGTCT 
TCCCGCCGAG CCGTGTCCCA GATTGTGACT GTTTATGATA AATTGCAAGA AAAAGTGGAG 
CTCTTATCCC GGAAGCTAAA CAGTGGAGAT AATCTGATAG TGGAGGAAGC AGTGCAGGAG 
CTGAACTCTT TCCTCGCACA GGAGAATATG AGGCTACAGG AATTGACAGA TCTTCTTCAG 
GAAAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTGC AGAGTAAAGT GGAGACAGCC 
GAATCACGAG TGTCTGTCCT GGAGTCCATG ATTGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAGCG ACTCAACCGA CACTTAGCAG AAGTCCTAGA ACGGGTGAAT 
TCCAAAGGTT ATAAGGTGTA TGGAGCGGGG AGCAGTCTGT ATGGCGGCAC AATCACTATC 
AATGCTCGGA AGTTTGAGGA AATGAATGCA GAGCTTGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCTCT GTGAGCTGGA GAAACTTCGG CAAGACTTTG AGGAGGTCAC TACACAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGTGCA GTGGAGCAAG TCGTTAAGGA AACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTC TTGTATAATG AGAGCCTACA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACCAGAG GAACCCACCA GCACCAGGTT 
GAGCTTATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTAATTCAG 
CTAGAAGATA CATTGGCCCA GGTCCGCAAG GAGTATGAAA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCTATAAACA GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTGAGATA TAAGCGGAAA 
TTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGCCTGC GTAGTGGTAG TGCCCTCCTG 
CAGTCCCAGT CTAGTACTGA GGACCCGAAG GATGAGCCTG CGGAGCTAAA ACCAGATTCT 
GAGGACTTAT CCTCCCAGTC CTCAGCTTCA AAGGCATCTC AGGAGGATGC CAATGAAATC 
AAGTCTAAAC GGGATGAAGA AGAACGAGAA CGAGAAAGGA GGGAGAAGGA GAGGGAACGA 
GAAAGAGAAC GGGAGAAGGA GAAGGAGAGA GAACGAGAGA AGCAGAAGCT AAAAGAGTCA 
GAAAAAGAGA GAGATTCTGC TAAGGATAAA GAGAAAGGCA AACATGATGA TGGACGGAAA 
AAGGAAGCAG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA GGAGAGCCAA 
AAGGAGATGA AACTATTGCT GGATATGTAC CGTTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCT AAGGCAGAGT TGGAAGATCT AAGGCAAAGA 
CTCAAGGATC TGGAAGATAA AGAGAAGAAA GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT GGAGGAGCAG ATAGAATACC TACAGAAGAA GCTAGCCATG 
GCCAAGCAGG AAGAAGAAGC ACTCCTCTCT GAAATGGATG TCACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTGATG CAGCAATTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCAGAGCG TATCAAGTCC AATCAGATCC ATAAGTTGCT TAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA GGTGTTGACT CTGAAGACTC AGGTTGATGC CCAGCTACAG 
GTAGTAAGGA AACTGGAAGA GAAGGAGCAT CTGTTACAGA GCAACATTGG CACAGGGGAG 
AAAGAGCTGG GTCTTAGGAC CCAAGCCTTA GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAA CTGGAGTTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAACAGTGTT ACCAAAGAAA AGGACATGTT CAATTTCAAA 
CGAGCCCAGG AGGACATCTC TAGACTTCGC AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTACCCA AGTGTGATGA GATTCTGATG GAAGAGATTA AGGATTACAA GGCACGCTTG 
ACCTGTCCGT GCTGTAACAT GCGTAAAAAG GATGCTGTTC TTACTAAGTG TTTTCATGTC 
TTCTGCTTTG AGTGTGTGAA GACACGCTAT GACACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCTT TTGGTGCCAA TGATTTTCAT CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062538.5
CDS	93..3020
Translation	MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASKASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	NM_019592.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 20 (RNF20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019592.6

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAACCTG GCACCTCCAT GCCTCCTGAG 
AAGAAGGCAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAGCT AGGAGGTGTC 
TCTTCAACGG AGGAACTAGA CATTAGAACA CTGCAAACCA AAAATCGCAA GCTGGCAGAA 
ATGTTGGATC AGCGGCAGGC CATTGAAGAT GAACTTCGTG AGCACATTGA AAAACTGGAA 
CGACGACAGG CCACTGATGA TGCCTCACTA TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGT TATGATCTGG AGCAGGGCTT GGGAGACCTA 
CTCACAGAAC GAAAAGCCCT TGTTGTGCCT GAACCAGAAC CAGACTCTGA TAGCAATCAG 
GAGCGTAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAGTCTCAGC TGCAGGAACG TGTGGAGTCT 
TCCCGCCGAG CCGTGTCCCA GATTGTGACT GTTTATGATA AATTGCAAGA AAAAGTGGAG 
CTCTTATCCC GGAAGCTAAA CAGTGGAGAT AATCTGATAG TGGAGGAAGC AGTGCAGGAG 
CTGAACTCTT TCCTCGCACA GGAGAATATG AGGCTACAGG AATTGACAGA TCTTCTTCAG 
GAAAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTGC AGAGTAAAGT GGAGACAGCC 
GAATCACGAG TGTCTGTCCT GGAGTCCATG ATTGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAGCG ACTCAACCGA CACTTAGCAG AAGTCCTAGA ACGGGTGAAT 
TCCAAAGGTT ATAAGGTGTA TGGAGCGGGG AGCAGTCTGT ATGGCGGCAC AATCACTATC 
AATGCTCGGA AGTTTGAGGA AATGAATGCA GAGCTTGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCTCT GTGAGCTGGA GAAACTTCGG CAAGACTTTG AGGAGGTCAC TACACAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGTGCA GTGGAGCAAG TCGTTAAGGA AACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTC TTGTATAATG AGAGCCTACA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACCAGAG GAACCCACCA GCACCAGGTT 
GAGCTTATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTAATTCAG 
CTAGAAGATA CATTGGCCCA GGTCCGCAAG GAGTATGAAA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCTATAAACA GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTGAGATA TAAGCGGAAA 
TTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGCCTGC GTAGTGGTAG TGCCCTCCTG 
CAGTCCCAGT CTAGTACTGA GGACCCGAAG GATGAGCCTG CGGAGCTAAA ACCAGATTCT 
GAGGACTTAT CCTCCCAGTC CTCAGCTTCA AAGGCATCTC AGGAGGATGC CAATGAAATC 
AAGTCTAAAC GGGATGAAGA AGAACGAGAA CGAGAAAGGA GGGAGAAGGA GAGGGAACGA 
GAAAGAGAAC GGGAGAAGGA GAAGGAGAGA GAACGAGAGA AGCAGAAGCT AAAAGAGTCA 
GAAAAAGAGA GAGATTCTGC TAAGGATAAA GAGAAAGGCA AACATGATGA TGGACGGAAA 
AAGGAAGCAG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA GGAGAGCCAA 
AAGGAGATGA AACTATTGCT GGATATGTAC CGTTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCT AAGGCAGAGT TGGAAGATCT AAGGCAAAGA 
CTCAAGGATC TGGAAGATAA AGAGAAGAAA GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT GGAGGAGCAG ATAGAATACC TACAGAAGAA GCTAGCCATG 
GCCAAGCAGG AAGAAGAAGC ACTCCTCTCT GAAATGGATG TCACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTGATG CAGCAATTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCAGAGCG TATCAAGTCC AATCAGATCC ATAAGTTGCT TAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA GGTGTTGACT CTGAAGACTC AGGTTGATGC CCAGCTACAG 
GTAGTAAGGA AACTGGAAGA GAAGGAGCAT CTGTTACAGA GCAACATTGG CACAGGGGAG 
AAAGAGCTGG GTCTTAGGAC CCAAGCCTTA GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAA CTGGAGTTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAACAGTGTT ACCAAAGAAA AGGACATGTT CAATTTCAAA 
CGAGCCCAGG AGGACATCTC TAGACTTCGC AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTACCCA AGTGTGATGA GATTCTGATG GAAGAGATTA AGGATTACAA GGCACGCTTG 
ACCTGTCCGT GCTGTAACAT GCGTAAAAAG GATGCTGTTC TTACTAAGTG TTTTCATGTC 
TTCTGCTTTG AGTGTGTGAA GACACGCTAT GACACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCTT TTGGTGCCAA TGATTTTCAT CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_011518862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517164.1 Homo sapiens ring finger protein 20 (RNF20), transcript variant X1, mRNA.

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CloneID	OHu29182
Clone ID Related Accession (Same CDS sequence)	NM_019592.7 , NM_019592.6 , XM_011518862.1
Accession Version	XM_011518862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAACCTG GCACCTCCAT GCCTCCTGAG 
AAGAAGGCAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAGCT AGGAGGTGTC 
TCTTCAACGG AGGAACTAGA CATTAGAACA CTGCAAACCA AAAATCGCAA GCTGGCAGAA 
ATGTTGGATC AGCGGCAGGC CATTGAAGAT GAACTTCGTG AGCACATTGA AAAACTGGAA 
CGACGACAGG CCACTGATGA TGCCTCACTA TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGT TATGATCTGG AGCAGGGCTT GGGAGACCTA 
CTCACAGAAC GAAAAGCCCT TGTTGTGCCT GAACCAGAAC CAGACTCTGA TAGCAATCAG 
GAGCGTAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAGTCTCAGC TGCAGGAACG TGTGGAGTCT 
TCCCGCCGAG CCGTGTCCCA GATTGTGACT GTTTATGATA AATTGCAAGA AAAAGTGGAG 
CTCTTATCCC GGAAGCTAAA CAGTGGAGAT AATCTGATAG TGGAGGAAGC AGTGCAGGAG 
CTGAACTCTT TCCTCGCACA GGAGAATATG AGGCTACAGG AATTGACAGA TCTTCTTCAG 
GAAAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTGC AGAGTAAAGT GGAGACAGCC 
GAATCACGAG TGTCTGTCCT GGAGTCCATG ATTGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAGCG ACTCAACCGA CACTTAGCAG AAGTCCTAGA ACGGGTGAAT 
TCCAAAGGTT ATAAGGTGTA TGGAGCGGGG AGCAGTCTGT ATGGCGGCAC AATCACTATC 
AATGCTCGGA AGTTTGAGGA AATGAATGCA GAGCTTGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCTCT GTGAGCTGGA GAAACTTCGG CAAGACTTTG AGGAGGTCAC TACACAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGTGCA GTGGAGCAAG TCGTTAAGGA AACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTC TTGTATAATG AGAGCCTACA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACCAGAG GAACCCACCA GCACCAGGTT 
GAGCTTATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTAATTCAG 
CTAGAAGATA CATTGGCCCA GGTCCGCAAG GAGTATGAAA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCTATAAACA GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTGAGATA TAAGCGGAAA 
TTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGCCTGC GTAGTGGTAG TGCCCTCCTG 
CAGTCCCAGT CTAGTACTGA GGACCCGAAG GATGAGCCTG CGGAGCTAAA ACCAGATTCT 
GAGGACTTAT CCTCCCAGTC CTCAGCTTCA AAGGCATCTC AGGAGGATGC CAATGAAATC 
AAGTCTAAAC GGGATGAAGA AGAACGAGAA CGAGAAAGGA GGGAGAAGGA GAGGGAACGA 
GAAAGAGAAC GGGAGAAGGA GAAGGAGAGA GAACGAGAGA AGCAGAAGCT AAAAGAGTCA 
GAAAAAGAGA GAGATTCTGC TAAGGATAAA GAGAAAGGCA AACATGATGA TGGACGGAAA 
AAGGAAGCAG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA GGAGAGCCAA 
AAGGAGATGA AACTATTGCT GGATATGTAC CGTTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCT AAGGCAGAGT TGGAAGATCT AAGGCAAAGA 
CTCAAGGATC TGGAAGATAA AGAGAAGAAA GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT GGAGGAGCAG ATAGAATACC TACAGAAGAA GCTAGCCATG 
GCCAAGCAGG AAGAAGAAGC ACTCCTCTCT GAAATGGATG TCACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTGATG CAGCAATTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCAGAGCG TATCAAGTCC AATCAGATCC ATAAGTTGCT TAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA GGTGTTGACT CTGAAGACTC AGGTTGATGC CCAGCTACAG 
GTAGTAAGGA AACTGGAAGA GAAGGAGCAT CTGTTACAGA GCAACATTGG CACAGGGGAG 
AAAGAGCTGG GTCTTAGGAC CCAAGCCTTA GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAA CTGGAGTTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAACAGTGTT ACCAAAGAAA AGGACATGTT CAATTTCAAA 
CGAGCCCAGG AGGACATCTC TAGACTTCGC AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTACCCA AGTGTGATGA GATTCTGATG GAAGAGATTA AGGATTACAA GGCACGCTTG 
ACCTGTCCGT GCTGTAACAT GCGTAAAAAG GATGCTGTTC TTACTAAGTG TTTTCATGTC 
TTCTGCTTTG AGTGTGTGAA GACACGCTAT GACACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCTT TTGGTGCCAA TGATTTTCAT CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517164.1
CDS	370..3297
Translation	MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASKASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_011518862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 20 (RNF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518862.1

ORF Insert Sequence:

ATGTCAGGAA TTGGAAATAA AAGAGCAGCT GGAGAACCTG GCACCTCCAT GCCTCCTGAG 
AAGAAGGCAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAGCT AGGAGGTGTC 
TCTTCAACGG AGGAACTAGA CATTAGAACA CTGCAAACCA AAAATCGCAA GCTGGCAGAA 
ATGTTGGATC AGCGGCAGGC CATTGAAGAT GAACTTCGTG AGCACATTGA AAAACTGGAA 
CGACGACAGG CCACTGATGA TGCCTCACTA TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAACGT TATGATCTGG AGCAGGGCTT GGGAGACCTA 
CTCACAGAAC GAAAAGCCCT TGTTGTGCCT GAACCAGAAC CAGACTCTGA TAGCAATCAG 
GAGCGTAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAGTCTCAGC TGCAGGAACG TGTGGAGTCT 
TCCCGCCGAG CCGTGTCCCA GATTGTGACT GTTTATGATA AATTGCAAGA AAAAGTGGAG 
CTCTTATCCC GGAAGCTAAA CAGTGGAGAT AATCTGATAG TGGAGGAAGC AGTGCAGGAG 
CTGAACTCTT TCCTCGCACA GGAGAATATG AGGCTACAGG AATTGACAGA TCTTCTTCAG 
GAAAAGCATC GCACCATGTC TCAGGAGTTC TCCAAGTTGC AGAGTAAAGT GGAGACAGCC 
GAATCACGAG TGTCTGTCCT GGAGTCCATG ATTGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGA GGGAACAGCG ACTCAACCGA CACTTAGCAG AAGTCCTAGA ACGGGTGAAT 
TCCAAAGGTT ATAAGGTGTA TGGAGCGGGG AGCAGTCTGT ATGGCGGCAC AATCACTATC 
AATGCTCGGA AGTTTGAGGA AATGAATGCA GAGCTTGAGG AGAACAAAGA GTTGGCTCAG 
AACCGTCTCT GTGAGCTGGA GAAACTTCGG CAAGACTTTG AGGAGGTCAC TACACAAAAT 
GAAAAGCTGA AGGTGGAATT GCGGAGTGCA GTGGAGCAAG TCGTTAAGGA AACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTC TTGTATAATG AGAGCCTACA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACCAGAG GAACCCACCA GCACCAGGTT 
GAGCTTATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACTGA AGTAATTCAG 
CTAGAAGATA CATTGGCCCA GGTCCGCAAG GAGTATGAAA TGCTGAGGAT AGAATTTGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCTATAAACA GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA TCACCAGCTG AAAGGGGAGG TCCTGAGATA TAAGCGGAAA 
TTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACACGCCTGC GTAGTGGTAG TGCCCTCCTG 
CAGTCCCAGT CTAGTACTGA GGACCCGAAG GATGAGCCTG CGGAGCTAAA ACCAGATTCT 
GAGGACTTAT CCTCCCAGTC CTCAGCTTCA AAGGCATCTC AGGAGGATGC CAATGAAATC 
AAGTCTAAAC GGGATGAAGA AGAACGAGAA CGAGAAAGGA GGGAGAAGGA GAGGGAACGA 
GAAAGAGAAC GGGAGAAGGA GAAGGAGAGA GAACGAGAGA AGCAGAAGCT AAAAGAGTCA 
GAAAAAGAGA GAGATTCTGC TAAGGATAAA GAGAAAGGCA AACATGATGA TGGACGGAAA 
AAGGAAGCAG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAGGCACA GGAGAGCCAA 
AAGGAGATGA AACTATTGCT GGATATGTAC CGTTCTGCCC CAAAGGAACA GAGAGACAAA 
GTTCAGCTGA TGGCAGCTGA GAAGAAGTCT AAGGCAGAGT TGGAAGATCT AAGGCAAAGA 
CTCAAGGATC TGGAAGATAA AGAGAAGAAA GAGAACAAGA AAATGGCTGA TGAGGATGCC 
TTGAGGAAGA TCCGGGCAGT GGAGGAGCAG ATAGAATACC TACAGAAGAA GCTAGCCATG 
GCCAAGCAGG AAGAAGAAGC ACTCCTCTCT GAAATGGATG TCACAGGCCA GGCCTTTGAA 
GACATGCAGG AGCAAAATAT CCGTTTGATG CAGCAATTGC GGGAGAAGGA TGATGCAAAT 
TTCAAGCTCA TGTCAGAGCG TATCAAGTCC AATCAGATCC ATAAGTTGCT TAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA GGTGTTGACT CTGAAGACTC AGGTTGATGC CCAGCTACAG 
GTAGTAAGGA AACTGGAAGA GAAGGAGCAT CTGTTACAGA GCAACATTGG CACAGGGGAG 
AAAGAGCTGG GTCTTAGGAC CCAAGCCTTA GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAA CTGGAGTTGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATCGTGGA GAACAGTGTT ACCAAAGAAA AGGACATGTT CAATTTCAAA 
CGAGCCCAGG AGGACATCTC TAGACTTCGC AGGAAGCTGG AGACCACAAA GAAACCAGAC 
AATGTACCCA AGTGTGATGA GATTCTGATG GAAGAGATTA AGGATTACAA GGCACGCTTG 
ACCTGTCCGT GCTGTAACAT GCGTAAAAAG GATGCTGTTC TTACTAAGTG TTTTCATGTC 
TTCTGCTTTG AGTGTGTGAA GACACGCTAT GACACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCTT TTGGTGCCAA TGATTTTCAT CGCATCTACA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagy-related protein ATG5 regulates histone H2B mono-ubiquitylation by translational control of RNF20.
Journal of genetics and genomics = Yi chuan xue bao44(10)503-506(2017 Oct)
Huang X,Yang L,Cai FF,Wang Y,Chen P,Mi J,Yu C,Lai J,Zhang X,Wei S,Cui W,Chen S

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RNF20 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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