MRAP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRAP
Entrez Gene ID	56246
Full Name	melanocortin 2 receptor accessory protein
Synonyms	B27,C21orf61,FALP,FGD2,GCCD2
General protein information	Preferred Names melanocortin 2 receptor accessory protein Names melanocortin-2 receptor accessory protein fat cell-specific low molecular weight protein fat tissue-specific low MW protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.11
Summary	This gene encodes a melanocortin receptor-interacting protein. The encoded protein regulates trafficking and function of the melanocortin 2 receptor in the adrenal gland. The encoded protein can also modulate signaling of other melanocortin receptors. Mutations in this gene have been associated with familial glucocorticoid deficiency type 2. Alternatively spliced transcript variants have been described. [provided by RefSeq, Dec 2009].
Disorder MIM:	609196
Disorder Html:	Glucocorticoid deficiency 2, 607398 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001285394.1	NP_001272323.1	melanocortin-2 receptor accessory protein isoform c
NM_178817.4	NP_848932.1	melanocortin-2 receptor accessory protein isoform alpha
XM_006724028.3	XP_006724091.1	melanocortin-2 receptor accessory protein isoform X1
XM_017028407.1	XP_016883896.1	melanocortin-2 receptor accessory protein isoform X2
XM_006724028.2	XP_006724091.1	melanocortin-2 receptor accessory protein isoform X1
NM_206898.1	NP_996781.1	melanocortin-2 receptor accessory protein isoform beta
NM_178817.3	NP_848932.1	melanocortin-2 receptor accessory protein isoform alpha
NM_001285394.2	NP_001272323.1	melanocortin-2 receptor accessory protein isoform c

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mrap	NP_001129306.1
	MRAP	XP_001096562.1
Bos taurus (cattle)	MRAP	XP_002684630.1
Homo sapiens (human)	MRAP	NP_848932.1
Pan troglodytes (chimpanzee)	MRAP	NP_001065291.2
Canis lupus familiaris (dog)	MRAP	XP_005638887.1
Mus musculus (house mouse)	Mrap	NP_084120.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Lipolytic Response and Energy Balance by Melanocortin 2 Receptor Accessory Protein (MRAP) in Adipocytes.
Zhang X, Saarinen AM, Campbell LE, De Filippis EA, Liu J
Diabetes67(2)222-234(2018 Feb)

Determination of alpha-2-MRAP gene polymorphisms in nephrolithiasis patients.
Mehde AA, Mehdi WA, Yusof F, Raus RA, Abidin ZAZ, Ghazali H, Rahman AA
International journal of biological macromolecules105(Pt 1)1324-1327(2017 Dec)

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gand?a J, Benleulmi-Chaachoua A, Tadagaki K, Oishi A, Wong V, Malty RH, Deineko V, Aoki H, Amin S, Yao Z, Morat? X, Otasek D, Kobayashi H, Menendez J, Auerbach D, Angers S, Pr?ulj N, Bouvier M, Babu M, Ciruela F, Jockers R, Jurisica I, Stagljar I
Molecular systems biology13(3)918(2017 03)

H295R expression of melanocortin 2 receptor accessory protein results in ACTH responsiveness.
Nanba K, Chen AX, Turcu AF, Rainey WE
Journal of molecular endocrinology56(2)69-76(2016 Feb)

Distinct melanocortin 2 receptor accessory protein domains are required for melanocortin 2 receptor interaction and promotion of receptor trafficking.
Webb TR, Chan L, Cooray SN, Cheetham ME, Chapple JP, Clark AJ
Endocrinology150(2)720-6(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MRAP expression in human cell line results in an ACTH responsive phenotype.
Title: H295R expression of melanocortin 2 receptor accessory protein results in ACTH responsiveness.

The results firmly establish that only the copy of MRAP oriented with the amino terminus on the extracellular side of the receptor is essential for Adrenocorticotropic Hormone signal transduction.
Title: Adrenocorticotropic Hormone (ACTH) Responses Require Actions of the Melanocortin-2 Receptor Accessory Protein on the Extracellular Surface of the Plasma Membrane.

Data show that co-expression with MRAPalpha, but not MRAP2, enhances MC4R constitutive activity. MRAPalpha-enhanced MC4R constitutive activity is not dependent on MC4R complex glycosylation but may result from MRAPalpha-induced changes in MC4R conformational states.
Title: hMRAP?, but Not hMRAP2, Enhances hMC4R Constitutive Activity in HEK293 Cells and This Is Not Dependent on hMRAP? Induced Changes in hMC4R Complex N-linked Glycosylation.

Data suggest that MRAP regulates expression of melanocortin receptors (MC1R-MC5R); MRAP is highly expressed in both zona fasciculata and undifferentiated zone of adrenal gland, sites of MC2R-mediated adrenal steroidogenesis. [REVIEW]
Title: Melanocortin receptor accessory proteins in adrenal gland physiology and beyond.

MRAP is positively regulated by ACTH and AngII in human adrenocortical tissues.
Title: Melanocortin 2 receptor-associated protein (MRAP) and MRAP2 in human adrenocortical tissues: regulation of expression and association with ACTH responsiveness.

The following MRAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01048	NM_001285394.2 Latest version!	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18593	NM_206898.1 Latest version!	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu29572	NM_178817.4 Latest version!	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18593	XM_017028407.1 Latest version!	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu29572	XM_006724028.3 Latest version!	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu29572	XM_006724028.2	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu29572	NM_178817.3	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01048	NM_001285394.1	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRAP ( NM_001285394.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001272323.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01048
Clone ID Related Accession (Same CDS sequence)	NM_001285394.1 , NM_001285394.2
Accession Version	NM_001285394.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF454916.1. Transcript Variant: This variant (3) lacks a coding exon compared to variant 1. The resulting isoform (c) has a shorter N-terminus compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF454916.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCTGGT CCGCCTCCCC GCAGATGAGG AACAGCCCCA AGCACCACCA AACATGCCCC 
TGGAGTCACG GCCTCAACCT CCACCTCTGC ATCCAGAAGT GCCTGCCGTG CCACAGGGAA 
CCCCTGGCAA CCTCACAGGC TCAGGCGAGC TCAGTGGAGC CAGGGAGCAG AACTGGCCCT 
GACCAGCCGC TACGACAGGA GAGCTCCTCC ACCTTGCCCC TCGGGGGTTT CCAGACCCAC 
CCCACTCTCC TCTGGGAACT GACCCTCAAT GGGGGTCCCC TCGTCAGGAG CAAGCCCAGC 
GAGCCTCCCC CTGGAGACAG GACCTCTCAA TTGCAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272323.1
CDS	249..590
Translation	MSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS

Target ORF information:

RefSeq Version	NM_001285394.1
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285394.1

ORF Insert Sequence:

ATGTCCTGGT CCGCCTCCCC GCAGATGAGG AACAGCCCCA AGCACCACCA AACATGCCCC 
TGGAGTCACG GCCTCAACCT CCACCTCTGC ATCCAGAAGT GCCTGCCGTG CCACAGGGAA 
CCCCTGGCAA CCTCACAGGC TCAGGCGAGC TCAGTGGAGC CAGGGAGCAG AACTGGCCCT 
GACCAGCCGC TACGACAGGA GAGCTCCTCC ACCTTGCCCC TCGGGGGTTT CCAGACCCAC 
CCCACTCTCC TCTGGGAACT GACCCTCAAT GGGGGTCCCC TCGTCAGGAG CAAGCCCAGC 
GAGCCTCCCC CTGGAGACAG GACCTCTCAA TTGCAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( NM_001285394.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001272323.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01048
Clone ID Related Accession (Same CDS sequence)	NM_001285394.1 , NM_001285394.2
Accession Version	NM_001285394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000266.1. On Apr 23, 2019 this sequence version replaced NM_001285394.1. Transcript Variant: This variant (3) lacks a coding exon compared to variant 1. The resulting isoform (c) has a shorter N-terminus compared to isoform alpha. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF454916.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCCTGGT CCGCCTCCCC GCAGATGAGG AACAGCCCCA AGCACCACCA AACATGCCCC 
TGGAGTCACG GCCTCAACCT CCACCTCTGC ATCCAGAAGT GCCTGCCGTG CCACAGGGAA 
CCCCTGGCAA CCTCACAGGC TCAGGCGAGC TCAGTGGAGC CAGGGAGCAG AACTGGCCCT 
GACCAGCCGC TACGACAGGA GAGCTCCTCC ACCTTGCCCC TCGGGGGTTT CCAGACCCAC 
CCCACTCTCC TCTGGGAACT GACCCTCAAT GGGGGTCCCC TCGTCAGGAG CAAGCCCAGC 
GAGCCTCCCC CTGGAGACAG GACCTCTCAA TTGCAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272323.1
CDS	249..590
Translation	MSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS

Target ORF information:

RefSeq Version	NM_001285394.2
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285394.2

ORF Insert Sequence:

ATGTCCTGGT CCGCCTCCCC GCAGATGAGG AACAGCCCCA AGCACCACCA AACATGCCCC 
TGGAGTCACG GCCTCAACCT CCACCTCTGC ATCCAGAAGT GCCTGCCGTG CCACAGGGAA 
CCCCTGGCAA CCTCACAGGC TCAGGCGAGC TCAGTGGAGC CAGGGAGCAG AACTGGCCCT 
GACCAGCCGC TACGACAGGA GAGCTCCTCC ACCTTGCCCC TCGGGGGTTT CCAGACCCAC 
CCCACTCTCC TCTGGGAACT GACCCTCAAT GGGGGTCCCC TCGTCAGGAG CAAGCCCAGC 
GAGCCTCCCC CTGGAGACAG GACCTCTCAA TTGCAGAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( NM_206898.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_996781.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18593
Clone ID Related Accession (Same CDS sequence)	XM_017028407.1 , NM_206898.1
Accession Version	NM_206898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF454915.1 and AF483549.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (beta) is shorter and has a distinct C-terminus compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF483549.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGCTTT AACACAGATG AATCTCTTCT GCATTCAGAA 
GTGCTGCCTC AAACTCGAGC TATTTCCTGT GATGAGCTCC AAGCCCCTAG AGAGGAAGGG 
GCGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996781.1
CDS	188..496
Translation	MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMSFNTDESLLHSEVLPQTRAISCDELQAPREEGAA

Target ORF information:

RefSeq Version	NM_206898.1
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206898.1

ORF Insert Sequence:

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGCTTT AACACAGATG AATCTCTTCT GCATTCAGAA 
GTGCTGCCTC AAACTCGAGC TATTTCCTGT GATGAGCTCC AAGCCCCTAG AGAGGAAGGG 
GCGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( NM_178817.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_848932.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29572
Clone ID Related Accession (Same CDS sequence)	NM_178817.4 , XM_006724028.3 , XM_006724028.2 , NM_178817.3
Accession Version	NM_178817.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000266.1. On Apr 23, 2019 this sequence version replaced NM_178817.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (alpha). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF454915.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848932.1
CDS	188..706
Translation	MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS

Target ORF information:

RefSeq Version	NM_178817.4
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178817.4

ORF Insert Sequence:

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( NM_178817.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_848932.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29572
Clone ID Related Accession (Same CDS sequence)	NM_178817.4 , XM_006724028.3 , XM_006724028.2 , NM_178817.3
Accession Version	NM_178817.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF454915.1 and AY079152.1. On Mar 30, 2004 this sequence version replaced NM_178817.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF454915.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848932.1
CDS	188..706
Translation	MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS

Target ORF information:

RefSeq Version	NM_178817.3
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178817.3

ORF Insert Sequence:

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( XM_017028407.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883896.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X2, mRNA.

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CloneID	OHu18593
Clone ID Related Accession (Same CDS sequence)	XM_017028407.1 , NM_206898.1
Accession Version	XM_017028407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGCTTT AACACAGATG AATCTCTTCT GCATTCAGAA 
GTGCTGCCTC AAACTCGAGC TATTTCCTGT GATGAGCTCC AAGCCCCTAG AGAGGAAGGG 
GCGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883896.1
CDS	114..422
Translation	MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMSFNTDESLLHSEVLPQTRAISCDELQAPREEGAA

Target ORF information:

RefSeq Version	XM_017028407.1
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028407.1

ORF Insert Sequence:

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGCTTT AACACAGATG AATCTCTTCT GCATTCAGAA 
GTGCTGCCTC AAACTCGAGC TATTTCCTGT GATGAGCTCC AAGCCCCTAG AGAGGAAGGG 
GCGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( XM_006724028.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724091.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu29572
Clone ID Related Accession (Same CDS sequence)	NM_178817.4 , XM_006724028.3 , XM_006724028.2 , NM_178817.3
Accession Version	XM_006724028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	melanocortin-2 receptor accessory protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724091.1
CDS	118..636
Translation	MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS

Target ORF information:

RefSeq Version	XM_006724028.3
Organism	Homo sapiens(human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724028.3

ORF Insert Sequence:

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRAP ( XM_006724028.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724091.1 Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu29572
Clone ID Related Accession (Same CDS sequence)	NM_178817.4 , XM_006724028.3 , XM_006724028.2 , NM_178817.3
Accession Version	XM_006724028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	melanocortin-2 receptor accessory protein isoform X1
Comment

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724091.1
Translation	MANGTNASAPYYSYEYYLDYLDLIPVDEKKLKAHKHSIVIAFWVSLAAFVVLLFLILLYMSWSASPQMRNSPKHHQTCPWSHGLNLHLCIQKCLPCHREPLATSQAQASSVEPGSRTGPDQPLRQESSSTLPLGGFQTHPTLLWELTLNGGPLVRSKPSEPPPGDRTSQLQS

Target ORF information:

RefSeq Version	XM_006724028.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens melanocortin 2 receptor accessory protein (MRAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724028.2

ORF Insert Sequence:

ATGGCCAACG GGACCAACGC CTCTGCCCCA TACTACAGCT ATGAATACTA CCTGGACTAT 
CTGGACCTCA TTCCCGTGGA CGAGAAGAAG CTGAAAGCCC ACAAACATTC CATCGTGATC 
GCATTCTGGG TGAGCCTGGC TGCCTTCGTG GTGCTGCTCT TCCTCATCTT GCTCTACATG 
TCCTGGTCCG CCTCCCCGCA GATGAGGAAC AGCCCCAAGC ACCACCAAAC ATGCCCCTGG 
AGTCACGGCC TCAACCTCCA CCTCTGCATC CAGAAGTGCC TGCCGTGCCA CAGGGAACCC 
CTGGCAACCT CACAGGCTCA GGCGAGCTCA GTGGAGCCAG GGAGCAGAAC TGGCCCTGAC 
CAGCCGCTAC GACAGGAGAG CTCCTCCACC TTGCCCCTCG GGGGTTTCCA GACCCACCCC 
ACTCTCCTCT GGGAACTGAC CCTCAATGGG GGTCCCCTCG TCAGGAGCAA GCCCAGCGAG 
CCTCCCCCTG GAGACAGGAC CTCTCAATTG CAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Lipolytic Response and Energy Balance by Melanocortin 2 Receptor Accessory Protein (MRAP) in Adipocytes.
Diabetes67(2)222-234(2018 Feb)
Zhang X,Saarinen AM,Campbell LE,De Filippis EA,Liu J

Determination of alpha-2-MRAP gene polymorphisms in nephrolithiasis patients.
International journal of biological macromolecules105(Pt 1)1324-1327(2017 Dec)
Mehde AA,Mehdi WA,Yusof F,Raus RA,Abidin ZAZ,Ghazali H,Rahman AA

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Molecular systems biology13(3)918(2017 03)
Sokolina K,Kittanakom S,Snider J,Kotlyar M,Maurice P,Gand?a J,Benleulmi-Chaachoua A,Tadagaki K,Oishi A,Wong V,Malty RH,Deineko V,Aoki H,Amin S,Yao Z,Morat? X,Otasek D,Kobayashi H,Menendez J,Auerbach D,Angers S,Pr?ulj N,Bouvier M,Babu M,Ciruela F,Jockers R,Jurisica I,Stagljar I

H295R expression of melanocortin 2 receptor accessory protein results in ACTH responsiveness.
Journal of molecular endocrinology56(2)69-76(2016 Feb)
Nanba K,Chen AX,Turcu AF,Rainey WE

Distinct melanocortin 2 receptor accessory protein domains are required for melanocortin 2 receptor interaction and promotion of receptor trafficking.
Endocrinology150(2)720-6(2009 Feb)
Webb TR,Chan L,Cooray SN,Cheetham ME,Chapple JP,Clark AJ

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MRAP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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