PCDHA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCDHA4
Entrez Gene ID	56144
Full Name	protocadherin alpha 4
Synonyms	CNR1,CNRN1,CRNR1,PCDH-ALPHA4
General protein information	Preferred Names protocadherin alpha 4 Names protocadherin alpha-4 KIAA0345-like 10 PCDH-alpha-4 ortholog of mouse CNR1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	606310

mRNA and Protein(s)

mRNA	Protein	Name
NM_031500.3	NP_113688.1	protocadherin alpha-4 isoform 2 precursor
NM_018907.4	NP_061730.1	protocadherin alpha-4 isoform 1 precursor
NM_031500.2	NP_113688.1	protocadherin alpha-4 isoform 2 precursor
NM_018907.3	NP_061730.1	protocadherin alpha-4 isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCDHA4	NP_061730.1
	PCDHA4	XP_001088407.1
Mus musculus (house mouse)	Pcdha4	NP_031792.1
Rattus norvegicus (Norway rat)	Pcdha4	NP_446385.1
Xenopus tropicalis (tropical clawed frog)	LOC101734840	XP_004913207.1
Bos taurus (cattle)	LOC787538	XP_005209574.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
Fox CS, Liu Y, White CC, Feitosa M, Smith AV, Heard-Costa N, Lohman K, , , , Johnson AD, Foster MC, Greenawalt DM, Griffin P, Ding J, Newman AB, Tylavsky F, Miljkovic I, Kritchevsky SB, Launer L, Garcia M, Eiriksdottir G, Carr JJ, Gudnason V, Harris TB, Cupples LA, Borecki IB
PLoS genetics8(5)e1002695(2012)

Comparative DNA sequence analysis of mouse and human protocadherin gene clusters.
Wu Q, Zhang T, Cheng JF, Kim Y, Grimwood J, Schmutz J, Dickson M, Noonan JP, Zhang MQ, Myers RM, Maniatis T
Genome research11(3)389-404(2001 Mar)

Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members.
Nollet F, Kools P, van Roy F
Journal of molecular biology299(3)551-72(2000 Jun)

Cadherin superfamily genes: functions, genomic organization, and neurologic diversity.
Yagi T, Takeichi M
Genes & development14(10)1169-80(2000 May)

Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes.
Wu Q, Maniatis T
Proceedings of the National Academy of Sciences of the United States of America97(7)3124-9(2000 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PCDHA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCDHA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00525	NM_031500.3 Latest version!	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu03141	NM_018907.4 Latest version!	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu00525	NM_031500.2	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu03141	NM_018907.3	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCDHA4 ( NM_031500.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_113688.1 Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00525
Clone ID Related Accession (Same CDS sequence)	NM_031500.3 , NM_031500.2
Accession Version	NM_031500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	protocadherin alpha-4 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005609.1, AB208925.1, BC112102.1 and KF458021.1. On May 31, 2019 this sequence version replaced NM_031500.2. Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929. ##Evidence-Data-START## Transcript is intronless :: BC113609.1, AF152482.1 [ECO:0000345] ##Evidence-Data-END##

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGGTTAG TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113688.1
CDS	121..2517
Translation	MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGRGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNLSIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFLVLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYSFSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDLEFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALVSYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDENDNAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGEISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLMAFSPSLPDSRDREDQLQTTEESFAKVSV

Target ORF information:

RefSeq Version	NM_031500.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031500.3

ORF Insert Sequence:

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGGTTAG TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCDHA4 ( NM_031500.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_113688.1 Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00525
Clone ID Related Accession (Same CDS sequence)	NM_031500.3 , NM_031500.2
Accession Version	NM_031500.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	protocadherin alpha-4 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB208925.1 and BC112102.1. On Jul 22, 2013 this sequence version replaced NM_031500.1. Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGGTTAG TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113688.1
CDS	115..2511
Translation	MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGRGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNLSIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFLVLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYSFSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDLEFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALVSYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDENDNAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGEISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLMAFSPSLPDSRDREDQLQTTEESFAKVSV

Target ORF information:

RefSeq Version	NM_031500.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031500.2

ORF Insert Sequence:

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGGTTAG TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCDHA4 ( NM_018907.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_061730.1 Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03141
Clone ID Related Accession (Same CDS sequence)	NM_018907.4 , NM_018907.3
Accession Version	NM_018907.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	protocadherin alpha-4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005609.1, AB208925.1, AF152312.1 and AC010223.6. On Nov 22, 2018 this sequence version replaced NM_018907.3. Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.8381.1, SRR1803613.230692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000530339.2/ ENSP00000435300.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGCCACG ACAGCCCAAC 
CCTGACTGGC GTTACTCTGC CTCCCTGAGA GCAGGCATGC ACAGCTCTGT GCACCTAGAG 
GAGGCTGGCA TTCTACGGGC TGGTCCAGGA GGGCCTGATC AGCAGTGGCC AACAGTATCC 
AGTGCAACAC CAGAACCAGA GGCAGGAGAA GTGTCCCCTC CAGTCGGTGC GGGTGTCAAC 
AGCAACAGCT GGACCTTTAA ATACGGACCA GGCAACCCCA AACAATCCGG TCCCGGTGAG 
TTGCCCGACA AATTCATTAT CCCAGGATCT CCTGCAATCA TCTCCATCCG GCAGGAGCCT 
ACTAACAGCC AAATTGACAA AAGTGACTTC ATAACCTTCG GCAAAAAGGA GGAGACCAAG 
AAAAAGAAGA AAAAGAAGAA GGGTAACAAG ACCCAGGAGA AAAAAGAGAA AGGGAACAGC 
ACGACTGACA ACAGTGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061730.1
CDS	121..2964
Translation	MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGRGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNLSIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFLVLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYSFSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDLEFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALVSYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDENDNAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGEISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLMAFSPSLPDSRDREDQLQTTEESFAKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ

Target ORF information:

RefSeq Version	NM_018907.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018907.4

ORF Insert Sequence:

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGCCACG ACAGCCCAAC 
CCTGACTGGC GTTACTCTGC CTCCCTGAGA GCAGGCATGC ACAGCTCTGT GCACCTAGAG 
GAGGCTGGCA TTCTACGGGC TGGTCCAGGA GGGCCTGATC AGCAGTGGCC AACAGTATCC 
AGTGCAACAC CAGAACCAGA GGCAGGAGAA GTGTCCCCTC CAGTCGGTGC GGGTGTCAAC 
AGCAACAGCT GGACCTTTAA ATACGGACCA GGCAACCCCA AACAATCCGG TCCCGGTGAG 
TTGCCCGACA AATTCATTAT CCCAGGATCT CCTGCAATCA TCTCCATCCG GCAGGAGCCT 
ACTAACAGCC AAATTGACAA AAGTGACTTC ATAACCTTCG GCAAAAAGGA GGAGACCAAG 
AAAAAGAAGA AAAAGAAGAA GGGTAACAAG ACCCAGGAGA AAAAAGAGAA AGGGAACAGC 
ACGACTGACA ACAGTGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCDHA4 ( NM_018907.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061730.1 Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03141
Clone ID Related Accession (Same CDS sequence)	NM_018907.4 , NM_018907.3
Accession Version	NM_018907.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	protocadherin alpha-4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB208925.1 and AF152312.1. On Jul 22, 2013 this sequence version replaced NM_018907.2. Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803611.167581.1, SRR1803613.230692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGCCACG ACAGCCCAAC 
CCTGACTGGC GTTACTCTGC CTCCCTGAGA GCAGGCATGC ACAGCTCTGT GCACCTAGAG 
GAGGCTGGCA TTCTACGGGC TGGTCCAGGA GGGCCTGATC AGCAGTGGCC AACAGTATCC 
AGTGCAACAC CAGAACCAGA GGCAGGAGAA GTGTCCCCTC CAGTCGGTGC GGGTGTCAAC 
AGCAACAGCT GGACCTTTAA ATACGGACCA GGCAACCCCA AACAATCCGG TCCCGGTGAG 
TTGCCCGACA AATTCATTAT CCCAGGATCT CCTGCAATCA TCTCCATCCG GCAGGAGCCT 
ACTAACAGCC AAATTGACAA AAGTGACTTC ATAACCTTCG GCAAAAAGGA GGAGACCAAG 
AAAAAGAAGA AAAAGAAGAA GGGTAACAAG ACCCAGGAGA AAAAAGAGAA AGGGAACAGC 
ACGACTGACA ACAGTGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061730.1
CDS	115..2958
Translation	MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGRGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNLSIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFLVLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYSFSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDLEFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALVSYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDENDNAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGEISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLMAFSPSLPDSRDREDQLQTTEESFAKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ

Target ORF information:

RefSeq Version	NM_018907.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protocadherin alpha 4 (PCDHA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018907.3

ORF Insert Sequence:

ATGGAGTTTT CCTGGGGAAG CGGCCAGGAA TCCCGGCGTC TGCTGCTCTT ACTTCTTCTC 
CTCGCAGCCT GGGAGGCAGG GAACGGTCAG CTCCACTACT CGGTCTCCGA GGAGGCCAAA 
CACGGCACCT TCGTGGGCCG CATCGCGCAG GACCTGGGAC TGGAGCTGGC GGAGCTGGTG 
CCGCGCCTGT TCCGGGTGGC GTCCAAGGGC CGCGGAGGCC TTCTGGAGGT AAATCTGCAG 
AATGGCATTT TGTTTGTGAA TTCTCGGATC GACCGGGAGG AGCTGTGCCG GCGGAGCGCG 
GAGTGCAGCA TCCACCTGGA GGTGATCGTA GACAGGCCGC TGCAGGTTTT CCATGTGGAC 
GTGGAGGTGA GGGACATTAA CGATAACCCG CCGGTGTTCC CAGCAACACA AAAGAACCTG 
TCCATCGCGG AATCCAGGCC GCTTGACTCT CGGTTTCCAC TAGAGGGCGC CTCGGATGCA 
GATATCGGGG AGAACGCCCT GCTCACTTAC AGACTGAGCC CAAATGAATA CTTTTCTCTG 
GAAAAACCAC CTGATGACGA GCTGGTAAAA GGTCTTGGGC TTATATTACG GAAATCTTTA 
GACAGAGAAG AAGCTCCGGA GATTTTTTTA GTGCTCACAG CCACTGATGG AGGCAAACCC 
GAGTTGACTG GCACCGTTCA GTTACTCATC ACAGTACTGG ATGCCAATGA CAATGCCCCA 
GCTTTTGACA GAACCATTTA TAAGGTGAGA TTACTAGAAA ATGTTCCTAA TGGAACATTG 
GTAATTAAAC TTAACGCCTC AGATTTAGAC GAAGGATTGA ATGGGGACAT TGTTTATTCA 
TTCTCAAATG ATATTTCGCC AAATGTGAAA TCCAAGTTTC ACATAGATCC AATTACTGGA 
CAAATTATTG TAAAGGGATA TATTGACTTT GAAGAAAGCA AATCCTATGA AATTATTGTA 
GAGGGCATTG ATAAGGGACA GCTCCCACTT TCTGGCCATT GTAGAGTTAT TGTGGAAGTA 
GAAGACAACA ACGATAATGT CCCAGATTTG GAATTCAAGT CTTTATCACT TCCAATTAGA 
GAGGACGCTC CACTGGGTAC AGTCATCGCC CTGATCAGCG TGTCCGACAA AGACATGGGT 
GTCAATGGGC TGGTCACCTG CTCCTTGACG TCCCACGTCC CCTTCAAGCT GGTGTCCACC 
TTCAAGAATT ACTACTCGTT GGTGCTGGAC AGTGCCCTGG ACCGCGAGAG CGTGTCAGCC 
TATGAGCTGG TGGTGACCGC GCGAGACGGG GGCTCGCCTT CGCTGTGGGC CACGGCCAGT 
GTTTCTGTGG AGGTGGCTGA TGTGAACGAC AACGCTCCGG CGTTCGCGCA GCCCGAGTAC 
ACAGTGTTCG TGAAGGAGAA CAACCCGCCG GGCTGCCACA TCTTCACTGT GTCTGCGTGG 
GACGCGGACG CGCAGGAGAA CGCGCTGGTG TCCTACTCGC TGGTAGAGCG GCGGGTAGGG 
GAGCGCGCGC TGTCGAGCTA CGTTTCGGTG CATGCGGAGA GCGGCAAGGT GTACGCGCTG 
CAGCCGCTGG ACCACGAGGA GCTAGAGCTG CTGCAGTTTC AGGTGACCGC TCGCGATGCC 
GGCGTGCCAC CTCTGGGCAG CAACGTGACG CTGCAGGTGT TCGTGCTGGA CGAAAACGAC 
AACGCGCCAG CACTGCTAGC GCCTCGGGCG GGTGGCACTG GTGGCGCAGT GAGCGAGCTG 
GTGCCATGGT CGGTGGGTGT GGGCCACGTG GTGGCAAAGG TGCGCGCGGT GGATGCTGAC 
TCGGGCTACA ACGCGTGGCT TTCGTACGAG CTGCAGCCGG GGACTGGTGG CGCGCGCATC 
CCGTTCCGCG TGGGGCTGTA CACTGGCGAG ATCAGCACAA CGCGTGCCCT GGACGAAACG 
GACGCTCCGC GCCACCGCCT ACTGGTACTG GTGAAGGACC ACGGCGAGCC CGCGCTGACG 
GCCACGGCCA CTGTGCTGGT GTCACTTGTG GAGAGTGGAC AGGCGCCAAA GGCCTCCTCA 
CGGGCGTTGG TGGGCGCTGT GGGTCCCGAT GCTGCGCTGG TGGATGTCAA CGTATACCTG 
ATCATTGCCA TCTGCGCGGT GTCCAGCCTT TTGGTGCTCA CGCTGCTGCT GTACACCGCG 
CTGCGGTGCT CTGCGCTGCC CACCGAGGGC GCGTGCGCTC CGGGCAAGCC CACGCTGGTG 
TGCTCCAGTG CGGTGGGGAG CTGGTCATAC TCGCAGCAGA GGAGGCCGAG GGTGTGCTCT 
GGTGAGGGCC CACCCAAGAC CGACCTCATG GCCTTCAGCC CCAGTTTACC TGACTCTAGG 
GACAGAGAAG ATCAGCTGCA GACAACTGAG GAATCCTTTG CAAAGCCACG ACAGCCCAAC 
CCTGACTGGC GTTACTCTGC CTCCCTGAGA GCAGGCATGC ACAGCTCTGT GCACCTAGAG 
GAGGCTGGCA TTCTACGGGC TGGTCCAGGA GGGCCTGATC AGCAGTGGCC AACAGTATCC 
AGTGCAACAC CAGAACCAGA GGCAGGAGAA GTGTCCCCTC CAGTCGGTGC GGGTGTCAAC 
AGCAACAGCT GGACCTTTAA ATACGGACCA GGCAACCCCA AACAATCCGG TCCCGGTGAG 
TTGCCCGACA AATTCATTAT CCCAGGATCT CCTGCAATCA TCTCCATCCG GCAGGAGCCT 
ACTAACAGCC AAATTGACAA AAGTGACTTC ATAACCTTCG GCAAAAAGGA GGAGACCAAG 
AAAAAGAAGA AAAAGAAGAA GGGTAACAAG ACCCAGGAGA AAAAAGAGAA AGGGAACAGC 
ACGACTGACA ACAGTGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
PLoS genetics8(5)e1002695(2012)
Fox CS,Liu Y,White CC,Feitosa M,Smith AV,Heard-Costa N,Lohman K,,,,Johnson AD,Foster MC,Greenawalt DM,Griffin P,Ding J,Newman AB,Tylavsky F,Miljkovic I,Kritchevsky SB,Launer L,Garcia M,Eiriksdottir G,Carr JJ,Gudnason V,Harris TB,Cupples LA,Borecki IB

Comparative DNA sequence analysis of mouse and human protocadherin gene clusters.
Genome research11(3)389-404(2001 Mar)
Wu Q,Zhang T,Cheng JF,Kim Y,Grimwood J,Schmutz J,Dickson M,Noonan JP,Zhang MQ,Myers RM,Maniatis T

Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members.
Journal of molecular biology299(3)551-72(2000 Jun)
Nollet F,Kools P,van Roy F

Cadherin superfamily genes: functions, genomic organization, and neurologic diversity.
Genes & development14(10)1169-80(2000 May)
Yagi T,Takeichi M

Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes.
Proceedings of the National Academy of Sciences of the United States of America97(7)3124-9(2000 Mar)
Wu Q,Maniatis T

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PCDHA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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