CFC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CFC1
Entrez Gene ID	55997
Full Name	cripto, FRL-1, cryptic family 1
Synonyms	CFC1B,CRYPTIC,DTGA2,HTX2
General protein information	Preferred Names cripto, FRL-1, cryptic family 1 Names cryptic protein cryptic family protein 1 heterotaxy 2 (autosomal dominant)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q21.1
Summary	This gene encodes a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family, which are involved in signalling during embryonic development. Proteins in this family share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. The protein encoded by this gene is necessary for patterning the left-right embryonic axis. Mutations in this gene are associated with defects in organ development, including autosomal visceral heterotaxy and congenital heart disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	605194
Disorder Html:	Heterotaxy, visceral, 2, autosomal, 605376 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_032545.3	NP_115934.1	cryptic protein isoform 1 precursor
XM_011511486.2	XP_011509788.1	cryptic protein isoform X1
NM_001270421.1	NP_001257350.1	cryptic protein isoform 3 precursor
NM_032545.4	NP_115934.1	cryptic protein isoform 1 precursor
XM_011511486.3	XP_011509788.1	cryptic protein isoform X1
NM_001270420.1	NP_001257349.1	cryptic protein isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC609557	XP_005631906.1
Mus musculus (house mouse)	Cfc1	NP_031711.1
Rattus norvegicus (Norway rat)	Cfc1	NP_001102774.1
Gallus gallus (chicken)	CFC1B	NP_990031.1
Pan troglodytes (chimpanzee)	CFC1	XP_001144170.1
Homo sapiens (human)	CFC1B	NP_001072998.1
Homo sapiens (human)	CFC1	NP_115934.1

Gene Ontology
Bibliography

Related articles in PubMed

CFC1 is a cancer stemness-regulating factor in neuroblastoma.
Chikaraishi K, Takenobu H, Sugino RP, Mukae K, Akter J, Haruta M, Kurosumi M, Endo TA, Koseki H, Shimojo N, Ohira M, Kamijo T
Oncotarget8(28)45046-45059(2017 Jul)

Left-right axis asymmetry determining human Cryptic gene is transcriptionally repressed by Snail.
Gupta K, Pilli VS, Aradhyam GK
BMC developmental biology16(1)39(2016 10)

Cripto-1 modulates macrophage cytokine secretion and phagocytic activity via NF-?B signaling.
Zhang DM, Bao YL, Yu CL, Wang YM, Song ZB
Immunologic research64(1)104-14(2016 Feb)

Duplication and deletion of CFC1 associated with heterotaxy syndrome.
Cao R, Long F, Wang L, Xu Y, Guo Y, Li F, Chen S, Sun K, Xu R
DNA and cell biology34(2)101-6(2015 Feb)

Molecular evolution of the EGF-CFC protein family.
Ravisankar V, Singh TP, Manoj N
Gene482(1-2)43-50(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrated that over expression of Snail suppresses Cryptic expression and confirmed that Snail directly binds to Cryptic gene promoter and regulates its expression.
Title: Left-right axis asymmetry determining human Cryptic gene is transcriptionally repressed by Snail.

enhances macrophage phagocytic activity and upregulates the production of anti- and pro-inflammatory cytokines via the NF-kappaB signaling pathway
Title: Cripto-1 modulates macrophage cytokine secretion and phagocytic activity via NF-?B signaling.

Data indicate that the duplication and deletion of CFC1 protein may play key roles in the occurrence of heterotaxy syndrome.
Title: Duplication and deletion of CFC1 associated with heterotaxy syndrome.

Observational study of gene-disease association. (HuGE Navigator)
Title: CFC1 mutations in Chinese children with congenital heart disease.

Observational study of gene-disease association. (HuGE Navigator)
Title: Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia.

The following CFC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55557	NM_001270420.1 Latest version!	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu55561	NM_001270421.1 Latest version!	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu69927	XM_011511486.3 Latest version!	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu20049	NM_032545.4 Latest version!	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu20049	NM_032545.3	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu69927	XM_011511486.2	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CFC1 ( NM_032545.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115934.1 Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20049
Clone ID Related Accession (Same CDS sequence)	NM_032545.3 , NM_032545.4
Accession Version	NM_032545.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	cryptic protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315326.1, AF312769.1, CA948246.1 and BI793242.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2012 this sequence version replaced NM_032545.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315326.1, AK290094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGGTG CGTCCGCGCG GCCGCGCTGC TGCAGGAACG GCGGTACCTG CGTGCTGGGC 
AGCTTCTGCG TGTGCCCGGC CCACTTCACC GGCCGCTACT GCGAGCATGA CCAGAGGCGC 
AGTGAATGCG GCGCCCTGGA GCACGGAGCC TGGACCCTCC GCGCCTGCCA CCTCTGCAGG 
TGCATCTTCG GGGCCCTGCA CTGCCTCCCC CTCCAGACGC CTGACCGCTG TGACCCGAAA 
GACTTCCTGG CCTCCCACGC TCACGGGCCG AGCGCCGGGG GCGCGCCCAG CCTGCTACTC 
TTGCTGCCCT GCGCACTCCT GCACCGCCTC CTGCGCCCGG ATGCGCCCGC GCACCCTCGG 
TCCCTGGTCC CTTCCGTCCT CCAGCGGGAG CGGCGCCCCT GCGGAAGGCC GGGACTTGGG 
CATCGCCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115934.1
CDS	289..960
Translation	MTWRHHVRLLFTVSLALQIINLGNSYQREKHNGGREEVTKVATQKHRQSPLNWTSSHFGEVTGSAEGWGPEEPLPYSRAFGEGASARPRCCRNGGTCVLGSFCVCPAHFTGRYCEHDQRRSECGALEHGAWTLRACHLCRCIFGALHCLPLQTPDRCDPKDFLASHAHGPSAGGAPSLLLLLPCALLHRLLRPDAPAHPRSLVPSVLQRERRPCGRPGLGHRL

Target ORF information:

RefSeq Version	NM_032545.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032545.3

ORF Insert Sequence:

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGGTG CGTCCGCGCG GCCGCGCTGC TGCAGGAACG GCGGTACCTG CGTGCTGGGC 
AGCTTCTGCG TGTGCCCGGC CCACTTCACC GGCCGCTACT GCGAGCATGA CCAGAGGCGC 
AGTGAATGCG GCGCCCTGGA GCACGGAGCC TGGACCCTCC GCGCCTGCCA CCTCTGCAGG 
TGCATCTTCG GGGCCCTGCA CTGCCTCCCC CTCCAGACGC CTGACCGCTG TGACCCGAAA 
GACTTCCTGG CCTCCCACGC TCACGGGCCG AGCGCCGGGG GCGCGCCCAG CCTGCTACTC 
TTGCTGCCCT GCGCACTCCT GCACCGCCTC CTGCGCCCGG ATGCGCCCGC GCACCCTCGG 
TCCCTGGTCC CTTCCGTCCT CCAGCGGGAG CGGCGCCCCT GCGGAAGGCC GGGACTTGGG 
CATCGCCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFC1 ( NM_001270420.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257349.1 Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55557
Clone ID Related Accession (Same CDS sequence)	NM_001270420.1
Accession Version	NM_001270420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cryptic protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315326.1, CA941621.1, BM310214.1, CA948246.1 and BI793242.1. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA941621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGTGA ATGCGGCGCC CTGGAGCACG GAGCCTGGAC CCTCCGCGCC TGCCACCTCT 
GCAGGTGCAT CTTCGGGGCC CTGCACTGCC TCCCCCTCCA GACGCCTGAC CGCTGTGACC 
CGAAAGACTT CCTGGCCTCC CACGCTCACG GGCCGAGCGC CGGGGGCGCG CCCAGCCTGC 
TACTCTTGCT GCCCTGCGCA CTCCTGCACC GCCTCCTGCG CCCGGATGCG CCCGCGCACC 
CTCGGTCCCT GGTCCCTTCC GTCCTCCAGC GGGAGCGGCG CCCCTGCGGA AGGCCGGGAC 
TTGGGCATCG CCTTTAATTT TCTATGTTGT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257349.1
CDS	289..864
Translation	MTWRHHVRLLFTVSLALQIINLGNSYQREKHNGGREEVTKVATQKHRQSPLNWTSSHFGEVTGSAEGWGPEEPLPYSRAFGEVNAAPWSTEPGPSAPATSAGASSGPCTASPSRRLTAVTRKTSWPPTLTGRAPGARPACYSCCPAHSCTASCARMRPRTLGPWSLPSSSGSGAPAEGRDLGIAFNFLCCK

Target ORF information:

RefSeq Version	NM_001270420.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270420.1

ORF Insert Sequence:

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGTGA ATGCGGCGCC CTGGAGCACG GAGCCTGGAC CCTCCGCGCC TGCCACCTCT 
GCAGGTGCAT CTTCGGGGCC CTGCACTGCC TCCCCCTCCA GACGCCTGAC CGCTGTGACC 
CGAAAGACTT CCTGGCCTCC CACGCTCACG GGCCGAGCGC CGGGGGCGCG CCCAGCCTGC 
TACTCTTGCT GCCCTGCGCA CTCCTGCACC GCCTCCTGCG CCCGGATGCG CCCGCGCACC 
CTCGGTCCCT GGTCCCTTCC GTCCTCCAGC GGGAGCGGCG CCCCTGCGGA AGGCCGGGAC 
TTGGGCATCG CCTTTAATTT TCTATGTTGT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFC1 ( NM_001270421.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257350.1 Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu55561
Clone ID Related Accession (Same CDS sequence)	NM_001270421.1
Accession Version	NM_001270421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	cryptic protein isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315326.1, CA777249.1, CA948246.1 and BI793242.1. Transcript Variant: This variant (3) lacks two exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA777249.1, CA867455.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGACC CGAAAGACTT CCTGGCCTCC CACGCTCACG GGCCGAGCGC CGGGGGCGCG 
CCCAGCCTGC TACTCTTGCT GCCCTGCGCA CTCCTGCACC GCCTCCTGCG CCCGGATGCG 
CCCGCGCACC CTCGGTCCCT GGTCCCTTCC GTCCTCCAGC GGGAGCGGCG CCCCTGCGGA 
AGGCCGGGAC TTGGGCATCG CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257350.1
CDS	289..735
Translation	MTWRHHVRLLFTVSLALQIINLGNSYQREKHNGGREEVTKVATQKHRQSPLNWTSSHFGEVTGSAEGWGPEEPLPYSRAFGEDPKDFLASHAHGPSAGGAPSLLLLLPCALLHRLLRPDAPAHPRSLVPSVLQRERRPCGRPGLGHRL

Target ORF information:

RefSeq Version	NM_001270421.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270421.1

ORF Insert Sequence:

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGACC CGAAAGACTT CCTGGCCTCC CACGCTCACG GGCCGAGCGC CGGGGGCGCG 
CCCAGCCTGC TACTCTTGCT GCCCTGCGCA CTCCTGCACC GCCTCCTGCG CCCGGATGCG 
CCCGCGCACC CTCGGTCCCT GGTCCCTTCC GTCCTCCAGC GGGAGCGGCG CCCCTGCGGA 
AGGCCGGGAC TTGGGCATCG CCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFC1 ( XM_011511486.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509788.1 Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69927
Clone ID Related Accession (Same CDS sequence)	XM_011511486.2 , XM_011511486.3
Accession Version	XM_011511486.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cryptic protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGATC TAGAGAACTG TGATTTAAAT GTGTTGAAAA TAAATATCCC AAGCCAAGAT 
AACAGCGGAC TCTTGCTTGG CTTCTCCCAG ACACCGCGTC CCCAGGAGGC CCCTCGCCGG 
TGGGTTTGGC GTCATCACAC CCGTGGGTGG TCCCGCCCAG GAGGCCTGTG CTTAATCACT 
GTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509788.1
CDS	284..712
Translation	MTWRHHVRLLFTVSLALQIINLGNSYQREKHNGGREEVTKVATQKHRQSPLNWTSSHFGEVTGSAEGWGPEEPLPYSRAFGEDLENCDLNVLKINIPSQDNSGLLLGFSQTPRPQEAPRRWVWRHHTRGWSRPGGLCLITVR

Target ORF information:

RefSeq Version	XM_011511486.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511486.2

ORF Insert Sequence:

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGATC TAGAGAACTG TGATTTAAAT GTGTTGAAAA TAAATATCCC AAGCCAAGAT 
AACAGCGGAC TCTTGCTTGG CTTCTCCCAG ACACCGCGTC CCCAGGAGGC CCCTCGCCGG 
TGGGTTTGGC GTCATCACAC CCGTGGGTGG TCCCGCCCAG GAGGCCTGTG CTTAATCACT 
GTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFC1 ( XM_011511486.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509788.1 Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69927
Clone ID Related Accession (Same CDS sequence)	XM_011511486.2 , XM_011511486.3
Accession Version	XM_011511486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cryptic protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511486.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGATC TAGAGAACTG TGATTTAAAT GTGTTGAAAA TAAATATCCC AAGCCAAGAT 
AACAGCGGAC TCTTGCTTGG CTTCTCCCAG ACACCGCGTC CCCAGGAGGC CCCTCGCCGG 
TGGGTTTGGC GTCATCACAC CCGTGGGTGG TCCCGCCCAG GAGGCCTGTG CTTAATCACT 
GTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509788.1
CDS	284..712
Translation	MTWRHHVRLLFTVSLALQIINLGNSYQREKHNGGREEVTKVATQKHRQSPLNWTSSHFGEVTGSAEGWGPEEPLPYSRAFGEDLENCDLNVLKINIPSQDNSGLLLGFSQTPRPQEAPRRWVWRHHTRGWSRPGGLCLITVR

Target ORF information:

RefSeq Version	XM_011511486.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511486.3

ORF Insert Sequence:

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGATC TAGAGAACTG TGATTTAAAT GTGTTGAAAA TAAATATCCC AAGCCAAGAT 
AACAGCGGAC TCTTGCTTGG CTTCTCCCAG ACACCGCGTC CCCAGGAGGC CCCTCGCCGG 
TGGGTTTGGC GTCATCACAC CCGTGGGTGG TCCCGCCCAG GAGGCCTGTG CTTAATCACT 
GTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFC1 ( NM_032545.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_115934.1 Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20049
Clone ID Related Accession (Same CDS sequence)	NM_032545.3 , NM_032545.4
Accession Version	NM_032545.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	cryptic protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315326.1, AF312769.1, CA948246.1 and BI793242.1. On Nov 30, 2019 this sequence version replaced NM_032545.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315326.1, BC110080.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGGTG CGTCCGCGCG GCCGCGCTGC TGCAGGAACG GCGGTACCTG CGTGCTGGGC 
AGCTTCTGCG TGTGCCCGGC CCACTTCACC GGCCGCTACT GCGAGCATGA CCAGAGGCGC 
AGTGAATGCG GCGCCCTGGA GCACGGAGCC TGGACCCTCC GCGCCTGCCA CCTCTGCAGG 
TGCATCTTCG GGGCCCTGCA CTGCCTCCCC CTCCAGACGC CTGACCGCTG TGACCCGAAA 
GACTTCCTGG CCTCCCACGC TCACGGGCCG AGCGCCGGGG GCGCGCCCAG CCTGCTACTC 
TTGCTGCCCT GCGCACTCCT GCACCGCCTC CTGCGCCCGG ATGCGCCCGC GCACCCTCGG 
TCCCTGGTCC CTTCCGTCCT CCAGCGGGAG CGGCGCCCCT GCGGAAGGCC GGGACTTGGG 
CATCGCCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115934.1
CDS	289..960
Translation	MTWRHHVRLLFTVSLALQIINLGNSYQREKHNGGREEVTKVATQKHRQSPLNWTSSHFGEVTGSAEGWGPEEPLPYSRAFGEGASARPRCCRNGGTCVLGSFCVCPAHFTGRYCEHDQRRSECGALEHGAWTLRACHLCRCIFGALHCLPLQTPDRCDPKDFLASHAHGPSAGGAPSLLLLLPCALLHRLLRPDAPAHPRSLVPSVLQRERRPCGRPGLGHRL

Target ORF information:

RefSeq Version	NM_032545.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cripto, FRL-1, cryptic family 1 (CFC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032545.4

ORF Insert Sequence:

ATGACCTGGA GGCACCATGT CAGGCTTCTG TTTACGGTCA GTTTGGCATT ACAGATCATC 
AATTTGGGAA ACAGCTATCA AAGAGAGAAA CATAACGGCG GTAGAGAGGA AGTCACCAAG 
GTTGCCACTC AGAAGCACCG ACAGTCACCG CTCAACTGGA CCTCCAGTCA TTTCGGAGAG 
GTGACTGGGA GCGCCGAGGG CTGGGGGCCG GAGGAGCCGC TCCCCTACTC CCGGGCTTTC 
GGAGAGGGTG CGTCCGCGCG GCCGCGCTGC TGCAGGAACG GCGGTACCTG CGTGCTGGGC 
AGCTTCTGCG TGTGCCCGGC CCACTTCACC GGCCGCTACT GCGAGCATGA CCAGAGGCGC 
AGTGAATGCG GCGCCCTGGA GCACGGAGCC TGGACCCTCC GCGCCTGCCA CCTCTGCAGG 
TGCATCTTCG GGGCCCTGCA CTGCCTCCCC CTCCAGACGC CTGACCGCTG TGACCCGAAA 
GACTTCCTGG CCTCCCACGC TCACGGGCCG AGCGCCGGGG GCGCGCCCAG CCTGCTACTC 
TTGCTGCCCT GCGCACTCCT GCACCGCCTC CTGCGCCCGG ATGCGCCCGC GCACCCTCGG 
TCCCTGGTCC CTTCCGTCCT CCAGCGGGAG CGGCGCCCCT GCGGAAGGCC GGGACTTGGG 
CATCGCCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CFC1 is a cancer stemness-regulating factor in neuroblastoma.
Oncotarget8(28)45046-45059(2017 Jul)
Chikaraishi K,Takenobu H,Sugino RP,Mukae K,Akter J,Haruta M,Kurosumi M,Endo TA,Koseki H,Shimojo N,Ohira M,Kamijo T

Left-right axis asymmetry determining human Cryptic gene is transcriptionally repressed by Snail.
BMC developmental biology16(1)39(2016 10)
Gupta K,Pilli VS,Aradhyam GK

Cripto-1 modulates macrophage cytokine secretion and phagocytic activity via NF-?B signaling.
Immunologic research64(1)104-14(2016 Feb)
Zhang DM,Bao YL,Yu CL,Wang YM,Song ZB

Duplication and deletion of CFC1 associated with heterotaxy syndrome.
DNA and cell biology34(2)101-6(2015 Feb)
Cao R,Long F,Wang L,Xu Y,Guo Y,Li F,Chen S,Sun K,Xu R

Molecular evolution of the EGF-CFC protein family.
Gene482(1-2)43-50(2011 Aug)
Ravisankar V,Singh TP,Manoj N

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CFC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報