MAPK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPK1
Entrez Gene ID	5594
Full Name	mitogen-activated protein kinase 1
Synonyms	ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK
General protein information	Preferred Names mitogen-activated protein kinase 1 Names mitogen-activated protein kinase 1 MAP kinase 1 MAP kinase 2 MAP kinase isoform p42 MAPK 2 extracellular signal-regulated kinase 2 mitogen-activated protein kinase 2 protein tyrosine kinase ERK2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.22
Summary	This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014].
Disorder MIM:	176948

mRNA and Protein(s)

mRNA	Protein	Name
NM_002745.4	NP_002736.3	mitogen-activated protein kinase 1
NM_002745.5	NP_002736.3	mitogen-activated protein kinase 1
NM_138957.3	NP_620407.1	mitogen-activated protein kinase 1

Pathways from BioSystems

Homologies

	MAPK1	XP_001089600.2
Bos taurus (cattle)	MAPK1	NP_786987.1
Gallus gallus (chicken)	MAPK1	NP_989481.1
Caenorhabditis elegans (roundworm)	mpk-1	NP_001022583.1
Oryza sativa (rice)	Os06g0154500	NP_001056846.1
Homo sapiens (human)	MAPK1	NP_002736.3
Canis lupus familiaris (dog)	MAPK1	NP_001104270.1
Rattus norvegicus (Norway rat)	Mapk1	NP_446294.1
Danio rerio (zebrafish)	mapk1	NP_878308.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009207	XP_319983.4
Xenopus tropicalis (tropical clawed frog)	mapk1	NP_001017127.1
Mus musculus (house mouse)	Mapk1	NP_001033752.1
Drosophila melanogaster (fruit fly)	rl	NP_001015122.1
Saccharomyces cerevisiae (baker's yeast)	KSS1	NP_011554.3
Eremothecium gossypii	AGOS_ACL191C	NP_983213.1
Arabidopsis thaliana (thale cress)	MPK6	NP_181907.1
Pan troglodytes (chimpanzee)	MAPK1	XP_515005.2
Kluyveromyces lactis	KLLA0A02497g	XP_451110.1

Gene Ontology

The following MAPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25335	NM_002745.5 Latest version!	Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu25335	NM_138957.3 Latest version!	Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu25335	NM_002745.4	Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPK1 ( NM_002745.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002736.3 Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mRNA.

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CloneID	OHu25335
Clone ID Related Accession (Same CDS sequence)	NM_002745.4 , NM_002745.5 , NM_138957.3
Accession Version	NM_002745.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-25
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017832.1, BC099905.1, AP000555.1, AP000554.1 and AP000553.1. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2005 this sequence version replaced NM_002745.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.48589.1, SRR1803613.210649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 19879846 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGCGGC GGGCGCGGGC CCGGAGATGG TCCGCGGGCA GGTGTTCGAC 
GTGGGGCCGC GCTACACCAA CCTCTCGTAC ATCGGCGAGG GCGCCTACGG CATGGTGTGC 
TCTGCTTATG ATAATGTCAA CAAAGTTCGA GTAGCTATCA AGAAAATCAG CCCCTTTGAG 
CACCAGACCT ACTGCCAGAG AACCCTGAGG GAGATAAAAA TCTTACTGCG CTTCAGACAT 
GAGAACATCA TTGGAATCAA TGACATTATT CGAGCACCAA CCATCGAGCA AATGAAAGAT 
GTATATATAG TACAGGACCT CATGGAAACA GATCTTTACA AGCTCTTGAA GACACAACAC 
CTCAGCAATG ACCATATCTG CTATTTTCTC TACCAGATCC TCAGAGGGTT AAAATATATC 
CATTCAGCTA ACGTTCTGCA CCGTGACCTC AAGCCTTCCA ACCTGCTGCT CAACACCACC 
TGTGATCTCA AGATCTGTGA CTTTGGCCTG GCCCGTGTTG CAGATCCAGA CCATGATCAC 
ACAGGGTTCC TGACAGAATA TGTGGCCACA CGTTGGTACA GGGCTCCAGA AATTATGTTG 
AATTCCAAGG GCTACACCAA GTCCATTGAT ATTTGGTCTG TAGGCTGCAT TCTGGCAGAA 
ATGCTTTCTA ACAGGCCCAT CTTTCCAGGG AAGCATTATC TTGACCAGCT GAACCACATT 
TTGGGTATTC TTGGATCCCC ATCACAAGAA GACCTGAATT GTATAATAAA TTTAAAAGCT 
AGGAACTATT TGCTTTCTCT TCCACACAAA AATAAGGTGC CATGGAACAG GCTGTTCCCA 
AATGCTGACT CCAAAGCTCT GGACTTATTG GACAAAATGT TGACATTCAA CCCACACAAG 
AGGATTGAAG TAGAACAGGC TCTGGCCCAC CCATATCTGG AGCAGTATTA CGACCCGAGT 
GACGAGCCCA TCGCCGAAGC ACCATTCAAG TTCGACATGG AATTGGATGA CTTGCCTAAG 
GAAAAGCTCA AAGAACTAAT TTTTGAAGAG ACTGCTAGAT TCCAGCCAGG ATACAGATCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002736.3
CDS	241..1323
Translation	MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS

Target ORF information:

RefSeq Version	NM_002745.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002745.4

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGGCGCGGGC CCGGAGATGG TCCGCGGGCA GGTGTTCGAC 
GTGGGGCCGC GCTACACCAA CCTCTCGTAC ATCGGCGAGG GCGCCTACGG CATGGTGTGC 
TCTGCTTATG ATAATGTCAA CAAAGTTCGA GTAGCTATCA AGAAAATCAG CCCCTTTGAG 
CACCAGACCT ACTGCCAGAG AACCCTGAGG GAGATAAAAA TCTTACTGCG CTTCAGACAT 
GAGAACATCA TTGGAATCAA TGACATTATT CGAGCACCAA CCATCGAGCA AATGAAAGAT 
GTATATATAG TACAGGACCT CATGGAAACA GATCTTTACA AGCTCTTGAA GACACAACAC 
CTCAGCAATG ACCATATCTG CTATTTTCTC TACCAGATCC TCAGAGGGTT AAAATATATC 
CATTCAGCTA ACGTTCTGCA CCGTGACCTC AAGCCTTCCA ACCTGCTGCT CAACACCACC 
TGTGATCTCA AGATCTGTGA CTTTGGCCTG GCCCGTGTTG CAGATCCAGA CCATGATCAC 
ACAGGGTTCC TGACAGAATA TGTGGCCACA CGTTGGTACA GGGCTCCAGA AATTATGTTG 
AATTCCAAGG GCTACACCAA GTCCATTGAT ATTTGGTCTG TAGGCTGCAT TCTGGCAGAA 
ATGCTTTCTA ACAGGCCCAT CTTTCCAGGG AAGCATTATC TTGACCAGCT GAACCACATT 
TTGGGTATTC TTGGATCCCC ATCACAAGAA GACCTGAATT GTATAATAAA TTTAAAAGCT 
AGGAACTATT TGCTTTCTCT TCCACACAAA AATAAGGTGC CATGGAACAG GCTGTTCCCA 
AATGCTGACT CCAAAGCTCT GGACTTATTG GACAAAATGT TGACATTCAA CCCACACAAG 
AGGATTGAAG TAGAACAGGC TCTGGCCCAC CCATATCTGG AGCAGTATTA CGACCCGAGT 
GACGAGCCCA TCGCCGAAGC ACCATTCAAG TTCGACATGG AATTGGATGA CTTGCCTAAG 
GAAAAGCTCA AAGAACTAAT TTTTGAAGAG ACTGCTAGAT TCCAGCCAGG ATACAGATCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPK1 ( NM_002745.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002736.3 Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25335
Clone ID Related Accession (Same CDS sequence)	NM_002745.4 , NM_002745.5 , NM_138957.3
Accession Version	NM_002745.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017832.1, BC099905.1, AP000555.1, AP000554.1 and AP000553.1. On Nov 20, 2019 this sequence version replaced NM_002745.4. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC099905.1, SRR1803613.210649.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 19879846 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGCGGC GGGCGCGGGC CCGGAGATGG TCCGCGGGCA GGTGTTCGAC 
GTGGGGCCGC GCTACACCAA CCTCTCGTAC ATCGGCGAGG GCGCCTACGG CATGGTGTGC 
TCTGCTTATG ATAATGTCAA CAAAGTTCGA GTAGCTATCA AGAAAATCAG CCCCTTTGAG 
CACCAGACCT ACTGCCAGAG AACCCTGAGG GAGATAAAAA TCTTACTGCG CTTCAGACAT 
GAGAACATCA TTGGAATCAA TGACATTATT CGAGCACCAA CCATCGAGCA AATGAAAGAT 
GTATATATAG TACAGGACCT CATGGAAACA GATCTTTACA AGCTCTTGAA GACACAACAC 
CTCAGCAATG ACCATATCTG CTATTTTCTC TACCAGATCC TCAGAGGGTT AAAATATATC 
CATTCAGCTA ACGTTCTGCA CCGTGACCTC AAGCCTTCCA ACCTGCTGCT CAACACCACC 
TGTGATCTCA AGATCTGTGA CTTTGGCCTG GCCCGTGTTG CAGATCCAGA CCATGATCAC 
ACAGGGTTCC TGACAGAATA TGTGGCCACA CGTTGGTACA GGGCTCCAGA AATTATGTTG 
AATTCCAAGG GCTACACCAA GTCCATTGAT ATTTGGTCTG TAGGCTGCAT TCTGGCAGAA 
ATGCTTTCTA ACAGGCCCAT CTTTCCAGGG AAGCATTATC TTGACCAGCT GAACCACATT 
TTGGGTATTC TTGGATCCCC ATCACAAGAA GACCTGAATT GTATAATAAA TTTAAAAGCT 
AGGAACTATT TGCTTTCTCT TCCACACAAA AATAAGGTGC CATGGAACAG GCTGTTCCCA 
AATGCTGACT CCAAAGCTCT GGACTTATTG GACAAAATGT TGACATTCAA CCCACACAAG 
AGGATTGAAG TAGAACAGGC TCTGGCCCAC CCATATCTGG AGCAGTATTA CGACCCGAGT 
GACGAGCCCA TCGCCGAAGC ACCATTCAAG TTCGACATGG AATTGGATGA CTTGCCTAAG 
GAAAAGCTCA AAGAACTAAT TTTTGAAGAG ACTGCTAGAT TCCAGCCAGG ATACAGATCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002736.3
CDS	206..1288
Translation	MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS

Target ORF information:

RefSeq Version	NM_002745.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002745.5

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGGCGCGGGC CCGGAGATGG TCCGCGGGCA GGTGTTCGAC 
GTGGGGCCGC GCTACACCAA CCTCTCGTAC ATCGGCGAGG GCGCCTACGG CATGGTGTGC 
TCTGCTTATG ATAATGTCAA CAAAGTTCGA GTAGCTATCA AGAAAATCAG CCCCTTTGAG 
CACCAGACCT ACTGCCAGAG AACCCTGAGG GAGATAAAAA TCTTACTGCG CTTCAGACAT 
GAGAACATCA TTGGAATCAA TGACATTATT CGAGCACCAA CCATCGAGCA AATGAAAGAT 
GTATATATAG TACAGGACCT CATGGAAACA GATCTTTACA AGCTCTTGAA GACACAACAC 
CTCAGCAATG ACCATATCTG CTATTTTCTC TACCAGATCC TCAGAGGGTT AAAATATATC 
CATTCAGCTA ACGTTCTGCA CCGTGACCTC AAGCCTTCCA ACCTGCTGCT CAACACCACC 
TGTGATCTCA AGATCTGTGA CTTTGGCCTG GCCCGTGTTG CAGATCCAGA CCATGATCAC 
ACAGGGTTCC TGACAGAATA TGTGGCCACA CGTTGGTACA GGGCTCCAGA AATTATGTTG 
AATTCCAAGG GCTACACCAA GTCCATTGAT ATTTGGTCTG TAGGCTGCAT TCTGGCAGAA 
ATGCTTTCTA ACAGGCCCAT CTTTCCAGGG AAGCATTATC TTGACCAGCT GAACCACATT 
TTGGGTATTC TTGGATCCCC ATCACAAGAA GACCTGAATT GTATAATAAA TTTAAAAGCT 
AGGAACTATT TGCTTTCTCT TCCACACAAA AATAAGGTGC CATGGAACAG GCTGTTCCCA 
AATGCTGACT CCAAAGCTCT GGACTTATTG GACAAAATGT TGACATTCAA CCCACACAAG 
AGGATTGAAG TAGAACAGGC TCTGGCCCAC CCATATCTGG AGCAGTATTA CGACCCGAGT 
GACGAGCCCA TCGCCGAAGC ACCATTCAAG TTCGACATGG AATTGGATGA CTTGCCTAAG 
GAAAAGCTCA AAGAACTAAT TTTTGAAGAG ACTGCTAGAT TCCAGCCAGG ATACAGATCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPK1 ( NM_138957.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_620407.1 Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25335
Clone ID Related Accession (Same CDS sequence)	NM_002745.4 , NM_002745.5 , NM_138957.3
Accession Version	NM_138957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017832.1 and BQ774391.1. This sequence is a reference standard in the RefSeqGene project. On Jan 29, 2014 this sequence version replaced NM_138957.2. Transcript Variant: This variant (2) contains a different 3' UTR region, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017832.1, SRR1163658.9884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 19879846 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGCGGC GGGCGCGGGC CCGGAGATGG TCCGCGGGCA GGTGTTCGAC 
GTGGGGCCGC GCTACACCAA CCTCTCGTAC ATCGGCGAGG GCGCCTACGG CATGGTGTGC 
TCTGCTTATG ATAATGTCAA CAAAGTTCGA GTAGCTATCA AGAAAATCAG CCCCTTTGAG 
CACCAGACCT ACTGCCAGAG AACCCTGAGG GAGATAAAAA TCTTACTGCG CTTCAGACAT 
GAGAACATCA TTGGAATCAA TGACATTATT CGAGCACCAA CCATCGAGCA AATGAAAGAT 
GTATATATAG TACAGGACCT CATGGAAACA GATCTTTACA AGCTCTTGAA GACACAACAC 
CTCAGCAATG ACCATATCTG CTATTTTCTC TACCAGATCC TCAGAGGGTT AAAATATATC 
CATTCAGCTA ACGTTCTGCA CCGTGACCTC AAGCCTTCCA ACCTGCTGCT CAACACCACC 
TGTGATCTCA AGATCTGTGA CTTTGGCCTG GCCCGTGTTG CAGATCCAGA CCATGATCAC 
ACAGGGTTCC TGACAGAATA TGTGGCCACA CGTTGGTACA GGGCTCCAGA AATTATGTTG 
AATTCCAAGG GCTACACCAA GTCCATTGAT ATTTGGTCTG TAGGCTGCAT TCTGGCAGAA 
ATGCTTTCTA ACAGGCCCAT CTTTCCAGGG AAGCATTATC TTGACCAGCT GAACCACATT 
TTGGGTATTC TTGGATCCCC ATCACAAGAA GACCTGAATT GTATAATAAA TTTAAAAGCT 
AGGAACTATT TGCTTTCTCT TCCACACAAA AATAAGGTGC CATGGAACAG GCTGTTCCCA 
AATGCTGACT CCAAAGCTCT GGACTTATTG GACAAAATGT TGACATTCAA CCCACACAAG 
AGGATTGAAG TAGAACAGGC TCTGGCCCAC CCATATCTGG AGCAGTATTA CGACCCGAGT 
GACGAGCCCA TCGCCGAAGC ACCATTCAAG TTCGACATGG AATTGGATGA CTTGCCTAAG 
GAAAAGCTCA AAGAACTAAT TTTTGAAGAG ACTGCTAGAT TCCAGCCAGG ATACAGATCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620407.1
CDS	241..1323
Translation	MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS

Target ORF information:

RefSeq Version	NM_138957.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138957.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGGCGCGGGC CCGGAGATGG TCCGCGGGCA GGTGTTCGAC 
GTGGGGCCGC GCTACACCAA CCTCTCGTAC ATCGGCGAGG GCGCCTACGG CATGGTGTGC 
TCTGCTTATG ATAATGTCAA CAAAGTTCGA GTAGCTATCA AGAAAATCAG CCCCTTTGAG 
CACCAGACCT ACTGCCAGAG AACCCTGAGG GAGATAAAAA TCTTACTGCG CTTCAGACAT 
GAGAACATCA TTGGAATCAA TGACATTATT CGAGCACCAA CCATCGAGCA AATGAAAGAT 
GTATATATAG TACAGGACCT CATGGAAACA GATCTTTACA AGCTCTTGAA GACACAACAC 
CTCAGCAATG ACCATATCTG CTATTTTCTC TACCAGATCC TCAGAGGGTT AAAATATATC 
CATTCAGCTA ACGTTCTGCA CCGTGACCTC AAGCCTTCCA ACCTGCTGCT CAACACCACC 
TGTGATCTCA AGATCTGTGA CTTTGGCCTG GCCCGTGTTG CAGATCCAGA CCATGATCAC 
ACAGGGTTCC TGACAGAATA TGTGGCCACA CGTTGGTACA GGGCTCCAGA AATTATGTTG 
AATTCCAAGG GCTACACCAA GTCCATTGAT ATTTGGTCTG TAGGCTGCAT TCTGGCAGAA 
ATGCTTTCTA ACAGGCCCAT CTTTCCAGGG AAGCATTATC TTGACCAGCT GAACCACATT 
TTGGGTATTC TTGGATCCCC ATCACAAGAA GACCTGAATT GTATAATAAA TTTAAAAGCT 
AGGAACTATT TGCTTTCTCT TCCACACAAA AATAAGGTGC CATGGAACAG GCTGTTCCCA 
AATGCTGACT CCAAAGCTCT GGACTTATTG GACAAAATGT TGACATTCAA CCCACACAAG 
AGGATTGAAG TAGAACAGGC TCTGGCCCAC CCATATCTGG AGCAGTATTA CGACCCGAGT 
GACGAGCCCA TCGCCGAAGC ACCATTCAAG TTCGACATGG AATTGGATGA CTTGCCTAAG 
GAAAAGCTCA AAGAACTAAT TTTTGAAGAG ACTGCTAGAT TCCAGCCAGG ATACAGATCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MAPK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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