MESP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MESP1
Entrez Gene ID	55897
Full Name	mesoderm posterior bHLH transcription factor 1
Synonyms	bHLHc5
General protein information	Preferred Names mesoderm posterior bHLH transcription factor 1 Names mesoderm posterior protein 1 class C basic helix-loop-helix protein 5 mesoderm posterior 1 homolog mesoderm posterior basic helix-loop-helix transcription factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q26.1
Disorder MIM:	608689

mRNA and Protein(s)

mRNA	Protein	Name
NM_018670.3	NP_061140.1	mesoderm posterior protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MESP1	XP_523151.3
	MESP1	XP_001093487.1
Canis lupus familiaris (dog)	MESP1	XP_003639018.1
Homo sapiens (human)	MESP1	NP_061140.1
Mus musculus (house mouse)	Mesp1	NP_032614.2
Rattus norvegicus (Norway rat)	Mesp1	NP_001101001.1

Gene Ontology
Bibliography

Related articles in PubMed

MESP1 loss?of?function mutation contributes to double outlet right ventricle.
Zhang M, Li FX, Liu XY, Huang RT, Xue S, Yang XX, Li YJ, Liu H, Shi HY, Pan X, Qiu XB, Yang YQ
Molecular medicine reports16(3)2747-2754(2017 Sep)

MESP1 Mutations in Patients with Congenital Heart Defects.
Werner P, Latney B, Deardorff MA, Goldmuntz E
Human mutation37(3)308-14(2016 Mar)

Dual reporter MESP1 mCherry/w-NKX2-5 eGFP/w hESCs enable studying early human cardiac differentiation.
Den Hartogh SC, Schreurs C, Monshouwer-Kloots JJ, Davis RP, Elliott DA, Mummery CL, Passier R
Stem cells (Dayton, Ohio)33(1)56-67(2015 Jan)

Mutational analysis of the human MESP1 gene in patients with congenital heart disease reveals a highly variable sequence in exon 1.
Lahm H, Deutsch MA, Dre?en M, Doppler S, Werner A, H?rer J, Cleuziou J, Schreiber C, B?hm J, Laugwitz KL, Lange R, Krane M
European journal of medical genetics56(11)591-8(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that pathologic variants in MESP1 may contribute to the development of congenital heart disease (CHD) and that additional protein partners and downstream targets could likewise contribute to the wide range of causes for CHD
Title: MESP1 Mutations in Patients with Congenital Heart Defects.

The MESP1-NKX2-5 hESC reporter line allows us to identify molecular cues crucial for specification and expansion of human cardiac mesoderm and early progenitors and their differentiation to specific cardiovascular derivatives.
Title: Dual reporter MESP1 mCherry/w-NKX2-5 eGFP/w hESCs enable studying early human cardiac differentiation.

MESP1 SNPs are associated with congenital heart disease in patients and altered transcription in vitro.
Title: Mutational analysis of the human MESP1 gene in patients with congenital heart disease reveals a highly variable sequence in exon 1.

Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors.
Title: Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors.

MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling
Title: MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling.

The following MESP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MESP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu28727	NM_018670.3 Latest version!	Homo sapiens mesoderm posterior bHLH transcription factor 1 (MESP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MESP1 ( NM_018670.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061140.1 Homo sapiens mesoderm posterior bHLH transcription factor 1 (MESP1), mRNA.

Price & Availability↑

CloneID	OHu28727
Clone ID Related Accession (Same CDS sequence)	NM_018670.3
Accession Version	NM_018670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	mesoderm posterior protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC006219.1, BP212916.1 and AL357533.1. On Jan 9, 2009 this sequence version replaced NM_018670.2. ##Evidence-Data-START## Transcript exon combination :: AL357533.1, BC006219.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC CCCTGTGCCC GCCGCTCTCC GAGTCCTGGA TGCTCTCTGC GGCCTGGGGC 
CCAACTCGGC GGCCGCCGCC CTCCGACAAG GACTGCGGCC GCTCCCTCGT CTCGTCCCCA 
GACTCATGGG GCAGCACCCC AGCCGACAGC CCCGTGGCGA GCCCCGCGCG GCCAGGCACC 
CTCCGGGACC CCCGCGCCCC CTCCGTAGGT AGGCGCGGCG CGCGCAGCAG CCGCCTGGGC 
AGCGGGCAGA GGCAGAGCGC CAGTGAGCGG GAGAAACTGC GCATGCGCAC GCTGGCCCGC 
GCCCTGCACG AGCTGCGCCG CTTTCTACCG CCGTCCGTGG CGCCCGCGGG CCAGAGCCTG 
ACCAAGATCG AGACGCTGCG CCTGGCTATC CGCTATATCG GCCACCTGTC GGCCGTGCTA 
GGCCTCAGCG AGGAGAGTCT CCAGCGCCGG TGCCGGCAGC GCGGTGACGC GGGGTCCCCT 
CGGGGCTGCC CGCTGTGCCC CGACGACTGC CCCGCGCAGA TGCAGACACG GACGCAGGCT 
GAGGGGCAGG GGCAGGGGCG CGGGCTGGGC CTGGTATCCG CCGTCCGCGC CGGGGCGTCC 
TGGGGATCCC CGCCTGCCTG CCCCGGAGCC CGAGCTGCAC CCGAGCCGCG CGACCCGCCT 
GCGCTGTTCG CCGAGGCGGC GTGCCCTGAA GGGCAGGCGA TGGAGCCAAG CCCACCGTCC 
CCGCTCCTTC CGGGCGACGT GCTGGCTCTG TTGGAGACCT GGATGCCCCT CTCGCCTCTG 
GAGTGGCTGC CTGAGGAGCC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061140.1
CDS	79..885
Translation	MAQPLCPPLSESWMLSAAWGPTRRPPPSDKDCGRSLVSSPDSWGSTPADSPVASPARPGTLRDPRAPSVGRRGARSSRLGSGQRQSASEREKLRMRTLARALHELRRFLPPSVAPAGQSLTKIETLRLAIRYIGHLSAVLGLSEESLQRRCRQRGDAGSPRGCPLCPDDCPAQMQTRTQAEGQGQGRGLGLVSAVRAGASWGSPPACPGARAAPEPRDPPALFAEAACPEGQAMEPSPPSPLLPGDVLALLETWMPLSPLEWLPEEPK

Target ORF information:

RefSeq Version	NM_018670.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mesoderm posterior bHLH transcription factor 1 (MESP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018670.3

ORF Insert Sequence:

ATGGCCCAGC CCCTGTGCCC GCCGCTCTCC GAGTCCTGGA TGCTCTCTGC GGCCTGGGGC 
CCAACTCGGC GGCCGCCGCC CTCCGACAAG GACTGCGGCC GCTCCCTCGT CTCGTCCCCA 
GACTCATGGG GCAGCACCCC AGCCGACAGC CCCGTGGCGA GCCCCGCGCG GCCAGGCACC 
CTCCGGGACC CCCGCGCCCC CTCCGTAGGT AGGCGCGGCG CGCGCAGCAG CCGCCTGGGC 
AGCGGGCAGA GGCAGAGCGC CAGTGAGCGG GAGAAACTGC GCATGCGCAC GCTGGCCCGC 
GCCCTGCACG AGCTGCGCCG CTTTCTACCG CCGTCCGTGG CGCCCGCGGG CCAGAGCCTG 
ACCAAGATCG AGACGCTGCG CCTGGCTATC CGCTATATCG GCCACCTGTC GGCCGTGCTA 
GGCCTCAGCG AGGAGAGTCT CCAGCGCCGG TGCCGGCAGC GCGGTGACGC GGGGTCCCCT 
CGGGGCTGCC CGCTGTGCCC CGACGACTGC CCCGCGCAGA TGCAGACACG GACGCAGGCT 
GAGGGGCAGG GGCAGGGGCG CGGGCTGGGC CTGGTATCCG CCGTCCGCGC CGGGGCGTCC 
TGGGGATCCC CGCCTGCCTG CCCCGGAGCC CGAGCTGCAC CCGAGCCGCG CGACCCGCCT 
GCGCTGTTCG CCGAGGCGGC GTGCCCTGAA GGGCAGGCGA TGGAGCCAAG CCCACCGTCC 
CCGCTCCTTC CGGGCGACGT GCTGGCTCTG TTGGAGACCT GGATGCCCCT CTCGCCTCTG 
GAGTGGCTGC CTGAGGAGCC CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MESP1 loss?of?function mutation contributes to double outlet right ventricle.
Molecular medicine reports16(3)2747-2754(2017 Sep)
Zhang M,Li FX,Liu XY,Huang RT,Xue S,Yang XX,Li YJ,Liu H,Shi HY,Pan X,Qiu XB,Yang YQ

MESP1 Mutations in Patients with Congenital Heart Defects.
Human mutation37(3)308-14(2016 Mar)
Werner P,Latney B,Deardorff MA,Goldmuntz E

Dual reporter MESP1 mCherry/w-NKX2-5 eGFP/w hESCs enable studying early human cardiac differentiation.
Stem cells (Dayton, Ohio)33(1)56-67(2015 Jan)
Den Hartogh SC,Schreurs C,Monshouwer-Kloots JJ,Davis RP,Elliott DA,Mummery CL,Passier R

Mutational analysis of the human MESP1 gene in patients with congenital heart disease reveals a highly variable sequence in exon 1.
European journal of medical genetics56(11)591-8(2013 Nov)
Lahm H,Deutsch MA,Dre?en M,Doppler S,Werner A,H?rer J,Cleuziou J,Schreiber C,B?hm J,Laugwitz KL,Lange R,Krane M

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MESP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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