CENPJ cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPJ
Entrez Gene ID	55835
Full Name	centromere protein J
Synonyms	BM032,CENP-J,CPAP,LAP,LIP1,MCPH6,SASS4,SCKL4,Sas-4
General protein information	Preferred Names centromere protein J Names centromere protein J LAG-3-associated protein LYST-interacting protein 1 LYST-interacting protein LIP1 LYST-interacting protein LIP7 centrosomal P4.1-associated protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.12-q12.13
Summary	This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012].
Disorder MIM:	609279
Disorder Html:	Microcephaly 6, primary, autosomal recessive, 608393 (3); ?Seckel syndrome 4, 613676 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017020673.1	XP_016876162.1	centromere protein J isoform X3
XM_011535149.2	XP_011533451.1	centromere protein J isoform X1
XM_011535150.2	XP_011533452.1	centromere protein J isoform X2
NM_018451.4	NP_060921.3	centromere protein J
NM_018451.5	NP_060921.3	centromere protein J

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CENPJ	XP_005635642.1
Pan troglodytes (chimpanzee)	CENPJ	NP_001029343.1
Bos taurus (cattle)	CENPJ	XP_005213884.1
Mus musculus (house mouse)	Cenpj	NP_001014996.2
Danio rerio (zebrafish)	cenpj	XP_697409.4
Xenopus tropicalis (tropical clawed frog)	LOC100491003	XP_004912130.1
	CENPJ	XP_001117666.2
Homo sapiens (human)	CENPJ	NP_060921.3
Rattus norvegicus (Norway rat)	Cenpj	NP_001100735.1
Gallus gallus (chicken)	CENPJ	XP_417152.4

Gene Ontology
Bibliography

Related articles in PubMed

CEP295 interacts with microtubules and is required for centriole elongation.
Chang CW, Hsu WB, Tsai JJ, Tang CJ, Tang TK
Journal of cell science129(13)2501-13(2016 07)

Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.
Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J, Li H
Nature communications711874(2016 Jun)

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.
Gabriel E, Wason A, Ramani A, Gooi LM, Keller P, Pozniakovsky A, Poser I, Noack F, Telugu NS, Calegari F, ?ari? T, Hescheler J, Hyman AA, Gottardo M, Callaini G, Alkuraya FS, Gopalakrishnan J
The EMBO journal35(8)803-19(2016 Apr)

Phosphorylation of CPAP by Aurora-A Maintains Spindle Pole Integrity during Mitosis.
Chou EJ, Hung LY, Tang CJ, Hsu WB, Wu HY, Liao PC, Tang TK
Cell reports14(12)2975-87(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CPAP promotes timely cilium disassembly to maintain neural progenitor pool. CPAP mutation causes Seckel syndrome with microcephaly.
Title: CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

CPAP-S467D protein has a low affinity for microtubule binding but a high affinity for pericentriolar material proteins.
Title: Phosphorylation of CPAP by Aurora-A Maintains Spindle Pole Integrity during Mitosis.

Data suggest that the single G-box domain (that appears to fold into 14-20 antiparallel beta-strands) of CENPJ has stable but dynamic structure; CRAP forms multimers (in solution and in crystals) of elongated fibrils similar to amyloid fibrils. [REVIEW]
Title: The centriolar protein CPAP G-box: an amyloid fibril in a single domain.

Centrobin plays a role in the stability and centriole elongation function of CPAP and limits the centriole length.
Title: Centrobin-mediated regulation of the centrosomal protein 4.1-associated protein (CPAP) level limits centriole length during elongation stage.

Centrobin-CPAP interaction is critical for the recruitment of CPAP to procentrioles to promote the elongation of daughter centrioles and for the persistence of CPAP on preexisting mother centrioles.
Title: Centrobin-centrosomal protein 4.1-associated protein (CPAP) interaction promotes CPAP localization to the centrioles during centriole duplication.

The following CENPJ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPJ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22286	NM_018451.5 Latest version!	Homo sapiens centromere protein J (CENPJ), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu84592	XM_017020673.1 Latest version!	Homo sapiens centromere protein J (CENPJ), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu69841	XM_011535149.2 Latest version!	Homo sapiens centromere protein J (CENPJ), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu69842	XM_011535150.2 Latest version!	Homo sapiens centromere protein J (CENPJ), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu22286	NM_018451.4	Homo sapiens centromere protein J (CENPJ), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPJ ( NM_018451.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060921.3 Homo sapiens centromere protein J (CENPJ), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22286
Clone ID Related Accession (Same CDS sequence)	NM_018451.4 , NM_018451.5
Accession Version	NM_018451.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	centromere protein J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062002.1, BC113664.1, AK292406.1, AJ303006.1, BU687310.1 and AL354798.13. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2012 this sequence version replaced NM_018451.3. Transcript Variant: This variant (1) represents the protein coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292406.1, SRR1660805.113334.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGAACA CATCTGTTCG ATTTCAAAAC 
AGTCAGATTT CTTCAGGAAC CCAGGTAGAA AAATACAAGA AAAATTATCT TCCAATGCAA 
GGCAATCCAC CTCGAAGATC CAAGTCTGCA CCTCCTCGTG ATTTAGGCAA TTTGGATAAG 
GGACAAGCTG CCTCTCCCAG GGAGCCACTT GAACCACTGA ACTTCCCAGA TCCTGAATAT 
AAAGAGGAGG AGGAAGACCA AGACATACAG GGAGAAATCA GTCATCCTGA TGGAAAGGTG 
GAAAAGGTTT ATAAGAATGG GTGCCGTGTT ATACTGTTTC CCAATGGAAC TCGAAAGGAA 
GTGAGTGCAG ATGGGAAGAC CATCACTGTC ACTTTCTTTA ATGGTGACGT GAAGCAGGTC 
ATGCCAGACC AAAGAGTGAT CTACTACTAT GCAGCTGCCC AGACCACTCA CACGACATAC 
CCGGAGGGAC TGGAAGTCTT ACATTTCTCA AGTGGACAAA TAGAAAAACA TTACCCAGAT 
GGAAGAAAAG AAATCACGTT TCCTGACCAG ACTGTTAAAA ACTTATTTCC TGATGGACAA 
GAAGAAAGCA TTTTCCCAGA TGGTACAATT GTCAGAGTAC AACGTGATGG CAACAAACTC 
ATAGAGTTTA ATAATGGCCA AAGAGAACTA CATACTGCCC AGTTCAAGAG ACGGGAATAC 
CCAGATGGCA CTGTTAAAAC CGTATATGCA AACGGTCATC AAGAAACGAA GTACAGATCC 
GGTCGGATAA GAGTTAAGGA CAAGGAGGGT AATGTGCTAA TGGACACGGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060921.3
CDS	196..4212
Translation	MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTEL

Target ORF information:

RefSeq Version	NM_018451.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein J (CENPJ), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018451.4

ORF Insert Sequence:

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGAACA CATCTGTTCG ATTTCAAAAC 
AGTCAGATTT CTTCAGGAAC CCAGGTAGAA AAATACAAGA AAAATTATCT TCCAATGCAA 
GGCAATCCAC CTCGAAGATC CAAGTCTGCA CCTCCTCGTG ATTTAGGCAA TTTGGATAAG 
GGACAAGCTG CCTCTCCCAG GGAGCCACTT GAACCACTGA ACTTCCCAGA TCCTGAATAT 
AAAGAGGAGG AGGAAGACCA AGACATACAG GGAGAAATCA GTCATCCTGA TGGAAAGGTG 
GAAAAGGTTT ATAAGAATGG GTGCCGTGTT ATACTGTTTC CCAATGGAAC TCGAAAGGAA 
GTGAGTGCAG ATGGGAAGAC CATCACTGTC ACTTTCTTTA ATGGTGACGT GAAGCAGGTC 
ATGCCAGACC AAAGAGTGAT CTACTACTAT GCAGCTGCCC AGACCACTCA CACGACATAC 
CCGGAGGGAC TGGAAGTCTT ACATTTCTCA AGTGGACAAA TAGAAAAACA TTACCCAGAT 
GGAAGAAAAG AAATCACGTT TCCTGACCAG ACTGTTAAAA ACTTATTTCC TGATGGACAA 
GAAGAAAGCA TTTTCCCAGA TGGTACAATT GTCAGAGTAC AACGTGATGG CAACAAACTC 
ATAGAGTTTA ATAATGGCCA AAGAGAACTA CATACTGCCC AGTTCAAGAG ACGGGAATAC 
CCAGATGGCA CTGTTAAAAC CGTATATGCA AACGGTCATC AAGAAACGAA GTACAGATCC 
GGTCGGATAA GAGTTAAGGA CAAGGAGGGT AATGTGCTAA TGGACACGGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPJ ( NM_018451.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060921.3 Homo sapiens centromere protein J (CENPJ), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22286
Clone ID Related Accession (Same CDS sequence)	NM_018451.4 , NM_018451.5
Accession Version	NM_018451.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	centromere protein J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354798.13. On Nov 23, 2018 this sequence version replaced NM_018451.4. Transcript Variant: This variant (1) represents the protein coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292406.1, SRR1660805.113334.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000381884.9/ ENSP00000371308.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGAACA CATCTGTTCG ATTTCAAAAC 
AGTCAGATTT CTTCAGGAAC CCAGGTAGAA AAATACAAGA AAAATTATCT TCCAATGCAA 
GGCAATCCAC CTCGAAGATC CAAGTCTGCA CCTCCTCGTG ATTTAGGCAA TTTGGATAAG 
GGACAAGCTG CCTCTCCCAG GGAGCCACTT GAACCACTGA ACTTCCCAGA TCCTGAATAT 
AAAGAGGAGG AGGAAGACCA AGACATACAG GGAGAAATCA GTCATCCTGA TGGAAAGGTG 
GAAAAGGTTT ATAAGAATGG GTGCCGTGTT ATACTGTTTC CCAATGGAAC TCGAAAGGAA 
GTGAGTGCAG ATGGGAAGAC CATCACTGTC ACTTTCTTTA ATGGTGACGT GAAGCAGGTC 
ATGCCAGACC AAAGAGTGAT CTACTACTAT GCAGCTGCCC AGACCACTCA CACGACATAC 
CCGGAGGGAC TGGAAGTCTT ACATTTCTCA AGTGGACAAA TAGAAAAACA TTACCCAGAT 
GGAAGAAAAG AAATCACGTT TCCTGACCAG ACTGTTAAAA ACTTATTTCC TGATGGACAA 
GAAGAAAGCA TTTTCCCAGA TGGTACAATT GTCAGAGTAC AACGTGATGG CAACAAACTC 
ATAGAGTTTA ATAATGGCCA AAGAGAACTA CATACTGCCC AGTTCAAGAG ACGGGAATAC 
CCAGATGGCA CTGTTAAAAC CGTATATGCA AACGGTCATC AAGAAACGAA GTACAGATCC 
GGTCGGATAA GAGTTAAGGA CAAGGAGGGT AATGTGCTAA TGGACACGGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060921.3
CDS	168..4184
Translation	MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTEL

Target ORF information:

RefSeq Version	NM_018451.5
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein J (CENPJ), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018451.5

ORF Insert Sequence:

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGAACA CATCTGTTCG ATTTCAAAAC 
AGTCAGATTT CTTCAGGAAC CCAGGTAGAA AAATACAAGA AAAATTATCT TCCAATGCAA 
GGCAATCCAC CTCGAAGATC CAAGTCTGCA CCTCCTCGTG ATTTAGGCAA TTTGGATAAG 
GGACAAGCTG CCTCTCCCAG GGAGCCACTT GAACCACTGA ACTTCCCAGA TCCTGAATAT 
AAAGAGGAGG AGGAAGACCA AGACATACAG GGAGAAATCA GTCATCCTGA TGGAAAGGTG 
GAAAAGGTTT ATAAGAATGG GTGCCGTGTT ATACTGTTTC CCAATGGAAC TCGAAAGGAA 
GTGAGTGCAG ATGGGAAGAC CATCACTGTC ACTTTCTTTA ATGGTGACGT GAAGCAGGTC 
ATGCCAGACC AAAGAGTGAT CTACTACTAT GCAGCTGCCC AGACCACTCA CACGACATAC 
CCGGAGGGAC TGGAAGTCTT ACATTTCTCA AGTGGACAAA TAGAAAAACA TTACCCAGAT 
GGAAGAAAAG AAATCACGTT TCCTGACCAG ACTGTTAAAA ACTTATTTCC TGATGGACAA 
GAAGAAAGCA TTTTCCCAGA TGGTACAATT GTCAGAGTAC AACGTGATGG CAACAAACTC 
ATAGAGTTTA ATAATGGCCA AAGAGAACTA CATACTGCCC AGTTCAAGAG ACGGGAATAC 
CCAGATGGCA CTGTTAAAAC CGTATATGCA AACGGTCATC AAGAAACGAA GTACAGATCC 
GGTCGGATAA GAGTTAAGGA CAAGGAGGGT AATGTGCTAA TGGACACGGA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPJ ( XM_017020673.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876162.1 Homo sapiens centromere protein J (CENPJ), transcript variant X5, mRNA.

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CloneID	OHu84592
Clone ID Related Accession (Same CDS sequence)	XM_017020673.1
Accession Version	XM_017020673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere protein J isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GCACATGGCT 
ACTGAGTACC TGCTGTGTAG CAGGCATATA CTGGCAATGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876162.1
CDS	196..3243
Translation	MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQHMATEYLLCSRHILAMQQ

Target ORF information:

RefSeq Version	XM_017020673.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein J (CENPJ), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020673.1

ORF Insert Sequence:

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GCACATGGCT 
ACTGAGTACC TGCTGTGTAG CAGGCATATA CTGGCAATGC AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPJ ( XM_011535149.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533451.1 Homo sapiens centromere protein J (CENPJ), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu69841
Clone ID Related Accession (Same CDS sequence)	XM_011535149.2
Accession Version	XM_011535149.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere protein J isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGAACA CATCTGTTCG ATTTCAAAAC 
AGTCAGATTT CTTCAGGAAC CCAGGTAGAA AAATACAAGA AAAATTATCT TCCAATGCAA 
GGCACAGCAC AGCCGCCTCA CACGCCCAGC AGCTTGTTGG CTGACCACCT GCCTCCGCTC 
TCCTCCTTCA GGCCATTCTC CCGTAACATC CAGCTCTGTT ACACTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533451.1
CDS	196..3606
Translation	MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGTAQPPHTPSSLLADHLPPLSSFRPFSRNIQLCYTF

Target ORF information:

RefSeq Version	XM_011535149.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein J (CENPJ), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535149.2

ORF Insert Sequence:

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGAACA CATCTGTTCG ATTTCAAAAC 
AGTCAGATTT CTTCAGGAAC CCAGGTAGAA AAATACAAGA AAAATTATCT TCCAATGCAA 
GGCACAGCAC AGCCGCCTCA CACGCCCAGC AGCTTGTTGG CTGACCACCT GCCTCCGCTC 
TCCTCCTTCA GGCCATTCTC CCGTAACATC CAGCTCTGTT ACACTTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPJ ( XM_011535150.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533452.1 Homo sapiens centromere protein J (CENPJ), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu69842
Clone ID Related Accession (Same CDS sequence)	XM_011535150.2
Accession Version	XM_011535150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere protein J isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGGTCC CACTGACTAG GAGCAACCGC 
AAGAAGGGAG CACATGGCCA CCAGGAACTC AGGCTTGGAC AGGGGAAGTT GAAATGCCTA 
CGAGACACAC AGGAGATCCG ACTAGGCAGC TGCACACGAC AGCACAGGCT CCAGTTGGGG 
ACACACACCT GGGATCGTCT CAATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533452.1
CDS	196..3585
Translation	MFLMPTSSELNSGQNFLTQWMTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINEEDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKDPLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTTGERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLKEENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEEQELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQLQRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKFENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLAVPLTRSNRKKGAHGHQELRLGQGKLKCLRDTQEIRLGSCTRQHRLQLGTHTWDRLNV

Target ORF information:

RefSeq Version	XM_011535150.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein J (CENPJ), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535150.2

ORF Insert Sequence:

ATGTTCCTGA TGCCAACCTC TTCAGAGTTA AACAGTGGGC AGAACTTCCT AACCCAGTGG 
ATGACCAATC CTTCTCGGGC TGGGGTCATA TTAAATCGTG GATTTCCTAT TTTGGAAGCA 
GACAAAGAGA AGCGAGCAGC TGTGGACATT TCTACCAGCT TTCCTATTAA AGGCACACAT 
TTTTCTGATA GCTTCAGCTT TATAAATGAA GAAGATTCAC TTCTTGAAGA ACAGAAGTTG 
GAGTCAAACA ACCCTTACAA ACCACAGTCA GATAAATCTG AAACCCATAC AGCCTTTCCT 
TGCATTAAAA AGGGACCACA GGTAGCGGCA TGTCACAGTG CTCCTGGACA CCAGGAAGAA 
AACAAAAATG ACTTCATCCC AGATCTTGCG AGTGAATTCA AAGAAGGGGC TTATAAAGAC 
CCACTTTTTA AAAAACTTGA ACAGCTGAAA GAAGTACAAC AGAAGAAGCA GGAACAATTG 
AAGAGGCAAC AGTTGGAGCA ACTACAGAGA CTCATGGAAG AACAAGAGAA GCTGCTCACC 
ATGGTGTCTG GGCAGTGCAC ACTTCCAGGT TTGAGTTTAC TGCCTGATGA TCAGAGCCAG 
AAGCACAGGT CTCCAGGAAA TACCACCACT GGAGAGAGAG CCACATGCTG CTTCCCATCA 
TATGTCTACC CGGACCCAAC CCAGGAAGAA ACATACCCGT CCAACATTTT ATCCCATGAG 
CAAAGCAACT TCTGTAGAAC TGCTCATGGA GATTTTGTCT TAACTTCAAA ACGTGCGTCT 
CCTAATTTAT TTTCTGAGGC ACAGTATCAA GAAGCACCTG TGGAAAAAAA TAATTTAAAA 
GAAGAAAACC GTAACCATCC TACAGGAGAA AGTATCTTAT GTTGGGAGAA AGTGACAGAA 
CAGATTCAGG AAGCAAATGA TAAGAACTTA CAAAAACATG ATGATTCCTC AGAAGTGGCT 
AATATTGAAG AAAGGCCCAT TAAAGCTGCT ATTGGAGAAA GGAAACAGAC CTTTGAAGAT 
TACTTAGAAG AACAAATTCA GTTGGAAGAG CAAGAACTGA AGCAAAAACA GCTGAAGGAA 
GCAGAAGGAC CATTGCCAAT CAAAGCAAAA CCAAAACAAC CATTTTTAAA ACGAGGAGAA 
GGTTTAGCTA GATTTACTAA TGCCAAATCT AAGTTTCAAA AAGGCAAAGA AAGTAAACTA 
GTGACTAACC AGAGCACTTC CGAGGACCAG CCGCTGTTTA AAATGGATAG ACAGCAACTC 
CAGCGGAAAA CCGCTCTTAA AAATAAAGAG CTGTGTGCAG ACAACCCTAT CCTTAAAAAG 
GACAGTAAAG CTAGAACCAA GAGTGGTTCT GTCACCCTCA GTCAGAAGCC GAAAATGCTG 
AAGTGTAGTA ACAGGAAAAG TCTTTCTCCG TCAGGATTGA AAATACAGAC GGGGAAGAAA 
TGTGATGGGC AGTTTAGAGA CCAGATCAAA TTTGAAAACA AAGTCACATC TAATAATAAA 
GAAAATGTAA CTGAGTGTCC AAAACCTTGC GATACTGGCT GCACAGGGTG GAATAAGACA 
CAAGGTAAAG ACAGACTTCC TCTTTCAACA GGGCCGGCCA GCCGGCTGGC TGCTAAGAGC 
CCCATAAGGG AGACCATGAA AGAGTCTGAA TCTTCTCTTG ACGTTTCTCT TCAGAAAAAG 
TTAGAGACTT GGGAACGAGA AAAGGAAAAG GAAAATTTGG AATTAGATGA ATTTTTGTTT 
TTAGAACAAG CTGCTGATGA AATATCATTT TCTAGTAATT CCTCATTTGT ACTGAAAATC 
TTAGAAAGGG ATCAACAGAT CTGCAAAGGT CACCGGATGT CTTCCACCCC TGTCAAAGCT 
GTGCCACAGA AGACAAATCC GGCAGATCCC ATTAGTCATT GTAACCGCAG TGAGGATTTG 
GACCACACTG CACGTGAGAA GGAGAGTGAG TGTGAAGTCG CACCCAAACA ACTTCATTCA 
TTGTCCTCAG CTGATGAATT GAGGGAACAG CCTTGTAAAA TCAGGAAAGC CGTCCAAAAG 
AGCACTTCTG AAAATCAGAC TGAATGGAAT GCACGTGACG ATGAAGGTGT TCCAAATAGT 
GACAGTAGCA CTGACTCTGA GGAACAGCTT GATGTTACCA TAAAACCATC GACTGAGGAT 
AGAGAGAGGG GCATCAGCAG CAGAGAGGAT AGCCCACAAG TCTGTGATGA TAAGGGGCCT 
TTTAAGGACA CCAGGACCCA AGAAGATAAA AGGAGAGATG TTGATCTGGA TTTGTCTGAT 
AAAGATTACA GTAGCGATGA GTCTATCATG GAAAGCATAA AACATAAAGT GTCTGAGCCC 
TCGAGATCCT CATCCCTAAG TCTGAGTAAA ATGGACTTTG ATGATGAAAG AACTTGGACT 
GACCTTGAAG AGAATTTGTG TAACCATGAT GTTGTTCTTG GGAATGAATC CACTTATGGG 
ACGCCGCAGA CATGCTACCC TAATAATGAA ATAGGTATCC TGGACAAAAC AATAAAAAGG 
AAGATTGCAC CAGTCAAGAG GGGAGAAGAC TTGAGCAAGT CCAGGAGGAG CAGAAGTCCT 
CCTACATCGG AGCTGATGAT GAAATTCTTT CCTTCTTTGA AACCAAAACC AAAGTCAGAT 
TCACACTTGG GAAATGAACT CAAGTTAAAC ATAAGTCAAG ACCAACCACC TGGTGACAAT 
GCTCGATCCC AGGTTTTGAG AGAGAAAATT ATTGAATTGG AAACAGAAAT AGAAAAGTTT 
AAAGCTGAGA ACGCATCTTT AGCTAAACTT CGCATTGAAC GAGAAAGTGC CTTGGAAAAA 
CTCAGGAAAG AAATTGCAGA CTTCGAACAA CAGAAAGCAA AAGAATTAGC TCGAATAGAA 
GAGTTTAAAA AGGAGGAGAT GAGGAAGCTA CAAAAGGAAC GTAAAGTTTT TGAAAAGTAT 
ACTACAGCTG CAAGAACTTT TCCAGATAAA AAGGAACGTG AAGAAATACA GACTTTAAAA 
CAGCAAATAG CAGATTTACG GGAAGATTTG AAAAGAAAGG AAACCAAATG GTCAAGTACA 
CACAGCCGTC TCAGAAGCCA GATACAAATG TTAGTCAGAG AGAACACAGA CCTCCGGGAA 
GAAATAAAAG TGATGGAAAG ATTCCGACTG GATGCCTGGA AGAGAGCAGA AGCCATAGAG 
AGCAGCCTCG AGGTGGAGAA GAAGGACAAG CTTGCGGTCC CACTGACTAG GAGCAACCGC 
AAGAAGGGAG CACATGGCCA CCAGGAACTC AGGCTTGGAC AGGGGAAGTT GAAATGCCTA 
CGAGACACAC AGGAGATCCG ACTAGGCAGC TGCACACGAC AGCACAGGCT CCAGTTGGGG 
ACACACACCT GGGATCGTCT CAATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CEP295 interacts with microtubules and is required for centriole elongation.
Journal of cell science129(13)2501-13(2016 07)
Chang CW,Hsu WB,Tsai JJ,Tang CJ,Tang TK

Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.
Nature communications711874(2016 Jun)
Zheng X,Ramani A,Soni K,Gottardo M,Zheng S,Ming Gooi L,Li W,Feng S,Mariappan A,Wason A,Widlund P,Pozniakovsky A,Poser I,Deng H,Ou G,Riparbelli M,Giuliano C,Hyman AA,Sattler M,Gopalakrishnan J,Li H

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.
The EMBO journal35(8)803-19(2016 Apr)
Gabriel E,Wason A,Ramani A,Gooi LM,Keller P,Pozniakovsky A,Poser I,Noack F,Telugu NS,Calegari F,?ari? T,Hescheler J,Hyman AA,Gottardo M,Callaini G,Alkuraya FS,Gopalakrishnan J

Phosphorylation of CPAP by Aurora-A Maintains Spindle Pole Integrity during Mitosis.
Cell reports14(12)2975-87(2016 Mar)
Chou EJ,Hung LY,Tang CJ,Hsu WB,Wu HY,Liao PC,Tang TK

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CENPJ cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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