PRKCG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCG
Entrez Gene ID	5582
Full Name	protein kinase C gamma
Synonyms	PKC-gamma,PKCC,PKCG,SCA14
General protein information	Preferred Names protein kinase C gamma Names protein kinase C gamma type
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.42
Summary	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015].
Disorder MIM:	176980
Disorder Html:	Spinocerebellar ataxia 14, 605361 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002739.4	NP_002730.1	protein kinase C gamma type isoform 2
NM_002739.5	NP_002730.1	protein kinase C gamma type isoform 2
NM_001316329.1	NP_001303258.1	protein kinase C gamma type isoform 1
NM_001316329.2	NP_001303258.1	protein kinase C gamma type isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRKCG	XP_003316702.2
Bos taurus (cattle)	PRKCG	NP_001159974.1
Danio rerio (zebrafish)	prkcg	XP_001921715.1
Canis lupus familiaris (dog)	LOC100855828	XP_003638850.1
Homo sapiens (human)	PRKCG	NP_002730.1
Rattus norvegicus (Norway rat)	Prkcg	NP_036760.1
Canis lupus familiaris (dog)	LOC484316	XP_541432.4
Mus musculus (house mouse)	Prkcg	NP_035232.1
Xenopus tropicalis (tropical clawed frog)	prkcg	XP_004916388.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased biological activity of protein Kinase C gamma is not required in Spinocerebellar ataxia 14.
Shimobayashi E, Kapfhammer JP
Molecular brain10(1)34(2017 07)

Calcium signaling, PKC gamma, IP3R1 and CAR8 link spinocerebellar ataxias and Purkinje cell dendritic development.
Shimobayashi E, Kapfhammer JP
Current neuropharmacology(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lysophosphatidylcholines prime polymorphonuclear neutrophil through Hck-dependent activation of PKCdelta, which stimulates PKCgamma, resulting in translocation of phosphorylated p47(phox).
Title: LysoPCs induce Hck- and PKC?-mediated activation of PKC? causing p47phox phosphorylation and membrane translocation in neutrophils.

The gene-environment combination of PRKCG rs3745406 C allele, BDNF rs6265 G allele and high level of negative life events was significantly associated with major depressive disorder.
Title: The interaction of combined effects of the BDNF and PRKCG genes and negative life events in major depressive disorder.

The results showed that carrier of rs454006*C allele and rs3745406*C might elevate the risk of osteosarcoma
Title: Genetic variations in the PRKCG gene and osteosarcoma risk in a Chinese population: a case-control study.

Data suggest that PRKCG (protein kinase C gamma) phosphorylates TA isoforms of p63 (tumor protein p63) at Thr157 to stabilize them and promote cell apoptosis in tumor cells.
Title: PKC? stabilizes TAp63 to promote cell apoptosis.

The rs454006 polymorphism of the PRKCG gene correlated to osteosarcoma susceptibility and might increase the risk of osteosarcoma.
Title: Single-nucleotide polymorphisms of the PRKCG gene and osteosarcoma susceptibility.

The following PRKCG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25732	NM_002739.5 Latest version!	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu59916	NM_001316329.2 Latest version!	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu25732	NM_002739.4	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu59916	NM_001316329.1	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCG ( NM_002739.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002730.1 Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25732
Clone ID Related Accession (Same CDS sequence)	NM_002739.4 , NM_002739.5
Accession Version	NM_002739.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	protein kinase C gamma type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC318717.1, BC047876.1 and AC008440.9. On Oct 15, 2015 this sequence version replaced NM_002739.3. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047876.1, SRR1803613.175793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002730.1
CDS	312..2405
Translation	MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Target ORF information:

RefSeq Version	NM_002739.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002739.4

ORF Insert Sequence:

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCG ( NM_002739.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002730.1 Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25732
Clone ID Related Accession (Same CDS sequence)	NM_002739.4 , NM_002739.5
Accession Version	NM_002739.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	protein kinase C gamma type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC318717.1, BC047876.1 and AC008440.9. On Nov 22, 2018 this sequence version replaced NM_002739.4. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.912204.1, SRR3476690.862624.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263431.4/ ENSP00000263431.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002730.1
CDS	299..2392
Translation	MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Target ORF information:

RefSeq Version	NM_002739.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002739.5

ORF Insert Sequence:

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCG ( NM_001316329.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303258.1 Homo sapiens protein kinase C gamma (PRKCG), transcript variant 1, mRNA.

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CloneID	OHu59916
Clone ID Related Accession (Same CDS sequence)	NM_001316329.1 , NM_001316329.2
Accession Version	NM_001316329.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	protein kinase C gamma type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC318717.1, AC008440.9, AK296434.1, AK312302.1 and BC047876.1. On Oct 15, 2015 this sequence version replaced XM_011527107.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296434.1, SRR1803614.188254.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT ATCCTGCACC 
CCAGCATTCC AGCTCTGCCC CCGCGGGTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303258.1
CDS	312..2444
Translation	MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVISCTPAFQLCPRGF

Target ORF information:

RefSeq Version	NM_001316329.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316329.1

ORF Insert Sequence:

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT ATCCTGCACC 
CCAGCATTCC AGCTCTGCCC CCGCGGGTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCG ( NM_001316329.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303258.1 Homo sapiens protein kinase C gamma (PRKCG), transcript variant 1, mRNA.

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CloneID	OHu59916
Clone ID Related Accession (Same CDS sequence)	NM_001316329.1 , NM_001316329.2
Accession Version	NM_001316329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	protein kinase C gamma type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC318717.1, AC008440.9, AK296434.1, AK312302.1 and BC047876.1. On May 31, 2019 this sequence version replaced NM_001316329.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296434.1, SRR1803614.188254.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT ATCCTGCACC 
CCAGCATTCC AGCTCTGCCC CCGCGGGTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303258.1
CDS	299..2431
Translation	MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVISCTPAFQLCPRGF

Target ORF information:

RefSeq Version	NM_001316329.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase C gamma (PRKCG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316329.2

ORF Insert Sequence:

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 
AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 
CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 
CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 
CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 
CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 
CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 
CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 
CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 
AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 
TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 
GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 
ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 
CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 
CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 
GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 
GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 
CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 
ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 
TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 
TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 
GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 
GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 
GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 
CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 
GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 
GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 
GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT ATCCTGCACC 
CCAGCATTCC AGCTCTGCCC CCGCGGGTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased biological activity of protein Kinase C gamma is not required in Spinocerebellar ataxia 14.
Molecular brain10(1)34(2017 07)
Shimobayashi E,Kapfhammer JP

Calcium signaling, PKC gamma, IP3R1 and CAR8 link spinocerebellar ataxias and Purkinje cell dendritic development.
Current neuropharmacology(2017 May)
Shimobayashi E,Kapfhammer JP

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PRKCG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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