METTL2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	METTL2B
Entrez Gene ID	55798
Full Name	methyltransferase like 2B
Synonyms	METL,METTL2,METTL2A,PSENIP1
General protein information	Preferred Names methyltransferase like 2B Names methyltransferase-like protein 2B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q32.1
Summary	This gene is a member of a family of methyltransferases that share homology with, but are distinct from, the UbiE family of methyltransferases. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	607846

mRNA and Protein(s)

mRNA	Protein	Name
NM_018396.3	NP_060866.2	tRNA N(3)-methylcytidine methyltransferase METTL2B
NM_018396.2	NP_060866.2	methyltransferase-like protein 2B

Pathways from BioSystems

Homologies

Homo sapiens (human)	METTL2B	NP_060866.2
Pan troglodytes (chimpanzee)	METTL2B	XP_001152439.1

Gene Ontology
Bibliography

Related articles in PubMed

Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae.
Noma A, Yi S, Katoh T, Takai Y, Suzuki T, Suzuki T
RNA (New York, N.Y.)17(6)1111-9(2011 Jun)

DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.
Zody MC, Garber M, Adams DJ, Sharpe T, Harrow J, Lupski JR, Nicholson C, Searle SM, Wilming L, Young SK, Abouelleil A, Allen NR, Bi W, Bloom T, Borowsky ML, Bugalter BE, Butler J, Chang JL, Chen CK, Cook A, Corum B, Cuomo CA, de Jong PJ, DeCaprio D, Dewar K, FitzGerald M, Gilbert J, Gibson R, Gnerre S, Goldstein S, Grafham DV, Grocock R, Hafez N, Hagopian DS, Hart E, Norman CH, Humphray S, Jaffe DB, Jones M, Kamal M, Khodiyar VK, LaButti K, Laird G, Lehoczky J, Liu X, Lokyitsang T, Loveland J, Lui A, Macdonald P, Major JE, Matthews L, Mauceli E, McCarroll SA, Mihalev AH, Mudge J, Nguyen C, Nicol R, O'Leary SB, Osoegawa K, Schwartz DC, Shaw-Smith C, Stankiewicz P, Steward C, Swarbreck D, Venkataraman V, Whittaker CA, Yang X, Zimmer AR, Bradley A, Hubbard T, Birren BW, Rogers J, Lander ES, Nusbaum C
Nature440(7087)1045-9(2006 Apr)

Identification of a novel family of putative methyltransferases that interact with human and Drosophila presenilins.
Zhang SX, Guo Y, Boulianne GL
Gene280(1-2)135-44(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following METTL2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the METTL2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10549	NM_018396.3 Latest version!	Homo sapiens methyltransferase like 2B (METTL2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu10549	NM_018396.2	Homo sapiens methyltransferase like 2B (METTL2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

METTL2B ( NM_018396.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060866.2 Homo sapiens methyltransferase like 2B (METTL2B), mRNA.

Price & Availability↑

CloneID	OHu10549
Clone ID Related Accession (Same CDS sequence)	NM_018396.3 , NM_018396.2
Accession Version	NM_018396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	tRNA N(3)-methylcytidine methyltransferase METTL2B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP247182.1, AC010655.7, AC090114.5 and BC064929.1. On Dec 2, 2019 this sequence version replaced NM_018396.2. ##Evidence-Data-START## Transcript exon combination :: AK302775.1, BC064929.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCT CCTACCCTGA AGGTGCACCT GCAATCCTCG CCGATAAGAG GCAGCAGTTC 
GGAAGCCGGT TCCTGAGCGA TCCGGCGCGC GTCTTCCACC ACAATGCCTG GGACAATGTG 
GAGTGGTCGG AAGAGCAAGC CGCGGCGGCG GAGAGAAAAG TCCAGGAGAA CAGTATCCAG 
CGGGTGTGCC AGGAGAAACA AGTTGATTAT GAGATCAATG CCCACAAATA CTGGAATGAC 
TTCTACAAAA TCCACGAAAA TGGGTTTTTC AAGGATAGAC ATTGGCTTTT TACCGAATTC 
CCTGAGCTGG CACCTAGCCA AAATCAAAAT CATTTGAAGG ATTGGTTCTT GGAGAACAAG 
AGTGAAGTAT GTGAATGTAG AAACAATGAG GATGGACCTG GTTTAATAAT GGAAGAACAG 
CACAAGTGTT CTTCGAAGAG CCTTGAACAT AAAACACAGA CACCTCCTGT GGAGGAGAAT 
GTAACTCAGA AAATTAGTGA CCTGGAAATT TGTGCTGATG AGTTTCCTGG ATCCTCAGCC 
ACCTACCGAA TACTGGAGGT TGGCTGTGGT GTGGGAAACA CAGTCTTTCC AATTTTACAA 
ACGAACAATG ACCCAGGACT CTTTGTTTAT TGCTGTGATT TTTCTTCCAC AGCTATAGAA 
CTGGTCCAGA CAAATTCAGA ATATGATCCT TCTCGGTGTT TTGCCTTTGT TCACGACCTG 
TGTGATGAAG AGAAGAGTTA CCCAGTGCCC AAGGGCAGTC TTGATATTAT CATTCTCATA 
TTTGTTCTTT CAGCAGTTGT TCCAGACAAG ATGCAGAAGG CTATCAACAG GCTGAGCAGG 
CTTCTGAAAC CTGGGGGGAT GGTACTTCTG CGAGATTACG GCCGCTATGA CATGGCTCAG 
CTTCGGTTTA AAAAAGGTCA GTGTCTATCT GGAAATTTCT ATGTGAGAGG TGATGGAACC 
AGAGTTTACT TCTTCACACA AGAGGAACTG GACACGCTTT TCACCACTGC TGGACTGGAA 
AAAGTTCAGA ATCTGGTGGA CCGCCGACTG CAGGTGAACC GAGGGAAGCA ACTGACAATG 
TACCGGGTTT GGATTCAGTG CAAATACTGC AAGCCCCTTC TGTCCAGCAC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060866.2
CDS	19..1155
Translation	MAGSYPEGAPAILADKRQQFGSRFLSDPARVFHHNAWDNVEWSEEQAAAAERKVQENSIQRVCQEKQVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAVVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS

Target ORF information:

RefSeq Version	NM_018396.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methyltransferase like 2B (METTL2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018396.3

ORF Insert Sequence:

ATGGCCGGCT CCTACCCTGA AGGTGCACCT GCAATCCTCG CCGATAAGAG GCAGCAGTTC 
GGAAGCCGGT TCCTGAGCGA TCCGGCGCGC GTCTTCCACC ACAATGCCTG GGACAATGTG 
GAGTGGTCGG AAGAGCAAGC CGCGGCGGCG GAGAGAAAAG TCCAGGAGAA CAGTATCCAG 
CGGGTGTGCC AGGAGAAACA AGTTGATTAT GAGATCAATG CCCACAAATA CTGGAATGAC 
TTCTACAAAA TCCACGAAAA TGGGTTTTTC AAGGATAGAC ATTGGCTTTT TACCGAATTC 
CCTGAGCTGG CACCTAGCCA AAATCAAAAT CATTTGAAGG ATTGGTTCTT GGAGAACAAG 
AGTGAAGTAT GTGAATGTAG AAACAATGAG GATGGACCTG GTTTAATAAT GGAAGAACAG 
CACAAGTGTT CTTCGAAGAG CCTTGAACAT AAAACACAGA CACCTCCTGT GGAGGAGAAT 
GTAACTCAGA AAATTAGTGA CCTGGAAATT TGTGCTGATG AGTTTCCTGG ATCCTCAGCC 
ACCTACCGAA TACTGGAGGT TGGCTGTGGT GTGGGAAACA CAGTCTTTCC AATTTTACAA 
ACGAACAATG ACCCAGGACT CTTTGTTTAT TGCTGTGATT TTTCTTCCAC AGCTATAGAA 
CTGGTCCAGA CAAATTCAGA ATATGATCCT TCTCGGTGTT TTGCCTTTGT TCACGACCTG 
TGTGATGAAG AGAAGAGTTA CCCAGTGCCC AAGGGCAGTC TTGATATTAT CATTCTCATA 
TTTGTTCTTT CAGCAGTTGT TCCAGACAAG ATGCAGAAGG CTATCAACAG GCTGAGCAGG 
CTTCTGAAAC CTGGGGGGAT GGTACTTCTG CGAGATTACG GCCGCTATGA CATGGCTCAG 
CTTCGGTTTA AAAAAGGTCA GTGTCTATCT GGAAATTTCT ATGTGAGAGG TGATGGAACC 
AGAGTTTACT TCTTCACACA AGAGGAACTG GACACGCTTT TCACCACTGC TGGACTGGAA 
AAAGTTCAGA ATCTGGTGGA CCGCCGACTG CAGGTGAACC GAGGGAAGCA ACTGACAATG 
TACCGGGTTT GGATTCAGTG CAAATACTGC AAGCCCCTTC TGTCCAGCAC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

METTL2B ( NM_018396.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060866.2 Homo sapiens methyltransferase like 2B (METTL2B), mRNA.

Price & Availability↑

CloneID	OHu10549
Clone ID Related Accession (Same CDS sequence)	NM_018396.3 , NM_018396.2
Accession Version	NM_018396.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	methyltransferase-like protein 2B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP247182.1, AC010655.7, AC090114.5 and BC064929.1. On Apr 21, 2006 this sequence version replaced NM_018396.1. ##Evidence-Data-START## Transcript exon combination :: BC064929.1, SRR1803616.147591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCT CCTACCCTGA AGGTGCACCT GCAATCCTCG CCGATAAGAG GCAGCAGTTC 
GGAAGCCGGT TCCTGAGCGA TCCGGCGCGC GTCTTCCACC ACAATGCCTG GGACAATGTG 
GAGTGGTCGG AAGAGCAAGC CGCGGCGGCG GAGAGAAAAG TCCAGGAGAA CAGTATCCAG 
CGGGTGTGCC AGGAGAAACA AGTTGATTAT GAGATCAATG CCCACAAATA CTGGAATGAC 
TTCTACAAAA TCCACGAAAA TGGGTTTTTC AAGGATAGAC ATTGGCTTTT TACCGAATTC 
CCTGAGCTGG CACCTAGCCA AAATCAAAAT CATTTGAAGG ATTGGTTCTT GGAGAACAAG 
AGTGAAGTAT GTGAATGTAG AAACAATGAG GATGGACCTG GTTTAATAAT GGAAGAACAG 
CACAAGTGTT CTTCGAAGAG CCTTGAACAT AAAACACAGA CACCTCCTGT GGAGGAGAAT 
GTAACTCAGA AAATTAGTGA CCTGGAAATT TGTGCTGATG AGTTTCCTGG ATCCTCAGCC 
ACCTACCGAA TACTGGAGGT TGGCTGTGGT GTGGGAAACA CAGTCTTTCC AATTTTACAA 
ACGAACAATG ACCCAGGACT CTTTGTTTAT TGCTGTGATT TTTCTTCCAC AGCTATAGAA 
CTGGTCCAGA CAAATTCAGA ATATGATCCT TCTCGGTGTT TTGCCTTTGT TCACGACCTG 
TGTGATGAAG AGAAGAGTTA CCCAGTGCCC AAGGGCAGTC TTGATATTAT CATTCTCATA 
TTTGTTCTTT CAGCAGTTGT TCCAGACAAG ATGCAGAAGG CTATCAACAG GCTGAGCAGG 
CTTCTGAAAC CTGGGGGGAT GGTACTTCTG CGAGATTACG GCCGCTATGA CATGGCTCAG 
CTTCGGTTTA AAAAAGGTCA GTGTCTATCT GGAAATTTCT ATGTGAGAGG TGATGGAACC 
AGAGTTTACT TCTTCACACA AGAGGAACTG GACACGCTTT TCACCACTGC TGGACTGGAA 
AAAGTTCAGA ATCTGGTGGA CCGCCGACTG CAGGTGAACC GAGGGAAGCA ACTGACAATG 
TACCGGGTTT GGATTCAGTG CAAATACTGC AAGCCCCTTC TGTCCAGCAC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060866.2
CDS	38..1174
Translation	MAGSYPEGAPAILADKRQQFGSRFLSDPARVFHHNAWDNVEWSEEQAAAAERKVQENSIQRVCQEKQVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAVVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS

Target ORF information:

RefSeq Version	NM_018396.2
Organism	Homo sapiens(human)
Definition	Homo sapiens methyltransferase like 2B (METTL2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018396.2

ORF Insert Sequence:

ATGGCCGGCT CCTACCCTGA AGGTGCACCT GCAATCCTCG CCGATAAGAG GCAGCAGTTC 
GGAAGCCGGT TCCTGAGCGA TCCGGCGCGC GTCTTCCACC ACAATGCCTG GGACAATGTG 
GAGTGGTCGG AAGAGCAAGC CGCGGCGGCG GAGAGAAAAG TCCAGGAGAA CAGTATCCAG 
CGGGTGTGCC AGGAGAAACA AGTTGATTAT GAGATCAATG CCCACAAATA CTGGAATGAC 
TTCTACAAAA TCCACGAAAA TGGGTTTTTC AAGGATAGAC ATTGGCTTTT TACCGAATTC 
CCTGAGCTGG CACCTAGCCA AAATCAAAAT CATTTGAAGG ATTGGTTCTT GGAGAACAAG 
AGTGAAGTAT GTGAATGTAG AAACAATGAG GATGGACCTG GTTTAATAAT GGAAGAACAG 
CACAAGTGTT CTTCGAAGAG CCTTGAACAT AAAACACAGA CACCTCCTGT GGAGGAGAAT 
GTAACTCAGA AAATTAGTGA CCTGGAAATT TGTGCTGATG AGTTTCCTGG ATCCTCAGCC 
ACCTACCGAA TACTGGAGGT TGGCTGTGGT GTGGGAAACA CAGTCTTTCC AATTTTACAA 
ACGAACAATG ACCCAGGACT CTTTGTTTAT TGCTGTGATT TTTCTTCCAC AGCTATAGAA 
CTGGTCCAGA CAAATTCAGA ATATGATCCT TCTCGGTGTT TTGCCTTTGT TCACGACCTG 
TGTGATGAAG AGAAGAGTTA CCCAGTGCCC AAGGGCAGTC TTGATATTAT CATTCTCATA 
TTTGTTCTTT CAGCAGTTGT TCCAGACAAG ATGCAGAAGG CTATCAACAG GCTGAGCAGG 
CTTCTGAAAC CTGGGGGGAT GGTACTTCTG CGAGATTACG GCCGCTATGA CATGGCTCAG 
CTTCGGTTTA AAAAAGGTCA GTGTCTATCT GGAAATTTCT ATGTGAGAGG TGATGGAACC 
AGAGTTTACT TCTTCACACA AGAGGAACTG GACACGCTTT TCACCACTGC TGGACTGGAA 
AAAGTTCAGA ATCTGGTGGA CCGCCGACTG CAGGTGAACC GAGGGAAGCA ACTGACAATG 
TACCGGGTTT GGATTCAGTG CAAATACTGC AAGCCCCTTC TGTCCAGCAC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae.
RNA (New York, N.Y.)17(6)1111-9(2011 Jun)
Noma A,Yi S,Katoh T,Takai Y,Suzuki T,Suzuki T

DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.
Nature440(7087)1045-9(2006 Apr)
Zody MC,Garber M,Adams DJ,Sharpe T,Harrow J,Lupski JR,Nicholson C,Searle SM,Wilming L,Young SK,Abouelleil A,Allen NR,Bi W,Bloom T,Borowsky ML,Bugalter BE,Butler J,Chang JL,Chen CK,Cook A,Corum B,Cuomo CA,de Jong PJ,DeCaprio D,Dewar K,FitzGerald M,Gilbert J,Gibson R,Gnerre S,Goldstein S,Grafham DV,Grocock R,Hafez N,Hagopian DS,Hart E,Norman CH,Humphray S,Jaffe DB,Jones M,Kamal M,Khodiyar VK,LaButti K,Laird G,Lehoczky J,Liu X,Lokyitsang T,Loveland J,Lui A,Macdonald P,Major JE,Matthews L,Mauceli E,McCarroll SA,Mihalev AH,Mudge J,Nguyen C,Nicol R,O'Leary SB,Osoegawa K,Schwartz DC,Shaw-Smith C,Stankiewicz P,Steward C,Swarbreck D,Venkataraman V,Whittaker CA,Yang X,Zimmer AR,Bradley A,Hubbard T,Birren BW,Rogers J,Lander ES,Nusbaum C

Identification of a novel family of putative methyltransferases that interact with human and Drosophila presenilins.
Gene280(1-2)135-44(2001 Dec)
Zhang SX,Guo Y,Boulianne GL