RIOK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RIOK2
Entrez Gene ID	55781
Full Name	RIO kinase 2
Synonyms	RIO2
General protein information	Preferred Names RIO kinase 2 Names serine/threonine-protein kinase RIO2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q15
Disorder MIM:	617754

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159749.2	NP_001153221.1	serine/threonine-protein kinase RIO2 isoform 2
NM_018343.3	NP_060813.2	serine/threonine-protein kinase RIO2 isoform 1
NM_001159749.1	NP_001153221.1	serine/threonine-protein kinase RIO2 isoform 2
NM_018343.2	NP_060813.2	serine/threonine-protein kinase RIO2 isoform 1
XM_017009628.1	XP_016865117.1	serine/threonine-protein kinase RIO2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RIOK2	NP_001006581.2
Danio rerio (zebrafish)	riok2	NP_998719.2
Canis lupus familiaris (dog)	RIOK2	XP_536291.3
Homo sapiens (human)	RIOK2	NP_060813.2
Pan troglodytes (chimpanzee)	RIOK2	XP_517843.2
Rattus norvegicus (Norway rat)	Riok2	NP_001009687.1
Arabidopsis thaliana (thale cress)	AT3G51270	NP_190695.2
Oryza sativa (rice)	Os01g0888700	NP_001045041.2
Drosophila melanogaster (fruit fly)	CG11859	NP_651365.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001526	XP_321599.4
Caenorhabditis elegans (roundworm)	riok-2	NP_493544.2
Saccharomyces cerevisiae (baker's yeast)	RIO2	NP_014192.1
Eremothecium gossypii	AGOS_ABL166W	NP_982781.1
Schizosaccharomyces pombe (fission yeast)	SPBC1703.05	NP_596200.2
Magnaporthe oryzae (rice blast fungus)	MGG_07119	XP_003715309.1
	RIOK2	XP_001095785.1
Bos taurus (cattle)	RIOK2	NP_001178220.1
Mus musculus (house mouse)	Riok2	NP_080210.1
Kluyveromyces lactis	KLLA0D19008g	XP_453904.1
Neurospora crassa	NCU07722	XP_961972.2
Xenopus tropicalis (tropical clawed frog)	riok2	NP_001016682.1

Gene Ontology
Bibliography

Related articles in PubMed

High Expression of RIOK2 and NOB1 Predict Human Non-small Cell Lung Cancer Outcomes.
Liu K, Chen HL, Wang S, Gu MM, Chen XM, Zhang SL, Yu KJ, You QS
Scientific reports628666(2016 Jun)

Discovery of selective RIO2 kinase small molecule ligand.
Varin T, Godfrey AG, Masquelin T, Nicolaou CA, Evans DA, Vieth M
Biochimica et biophysica acta1854(10 Pt B)1630-6(2015 Oct)

A kinome-wide RNAi screen in Drosophila Glia reveals that the RIO kinases mediate cell proliferation and survival through TORC2-Akt signaling in glioblastoma.
Read RD, Fenton TR, Gomez GG, Wykosky J, Vandenberg SR, Babic I, Iwanami A, Yang H, Cavenee WK, Mischel PS, Furnari FB, Thomas JB
PLoS genetics9(2)e1003253(2013)

Phosphorylation of right open reading frame 2 (Rio2) protein kinase by polo-like kinase 1 regulates mitotic progression.
Liu T, Deng M, Li J, Tong X, Wei Q, Ye X
The Journal of biological chemistry286(42)36352-60(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

As RIO2 is involved in the biosynthesis of the ribosome and cell cycle regulation, our selective ligand may be useful for the delineation of the biological role of this kinase.
Title: Discovery of selective RIO2 kinase small molecule ligand.

reduced expression of RIOK1 or RIOK2 disrupted Akt signaling and caused cell cycle exit, apoptosis, and chemosensitivity in glioblastoma cells
Title: A kinome-wide RNAi screen in Drosophila Glia reveals that the RIO kinases mediate cell proliferation and survival through TORC2-Akt signaling in glioblastoma.

Plk1-mediated phosphorylation of Rio2 regulates metaphase-anaphase transition during mitotic progression.
Title: Phosphorylation of right open reading frame 2 (Rio2) protein kinase by polo-like kinase 1 regulates mitotic progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: The ERAP2 gene is associated with preeclampsia in Australian and Norwegian populations.

Results show that the protein kinase human Rio2 (hRio2) is part of a late 40S preribosomal particle in human cells.
Title: Distinct cytoplasmic maturation steps of 40S ribosomal subunit precursors require hRio2.

The following RIOK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIOK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00091	NM_018343.3 Latest version!	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu03064	NM_001159749.2 Latest version!	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00
OHu95944	XM_017009628.1 Latest version!	Homo sapiens RIO kinase 2 (RIOK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu03064	NM_001159749.1	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00
OHu00091	NM_018343.2	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIOK2 ( NM_018343.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060813.2 Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00091
Clone ID Related Accession (Same CDS sequence)	NM_018343.3 , NM_018343.2
Accession Version	NM_018343.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB240299.1, AK002021.1, AC008865.3 and CA415109.1. On Nov 23, 2018 this sequence version replaced NM_018343.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.70800.1, SRR1803611.277357.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000283109.8/ ENSP00000283109.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGAGA TGAAGAAAAT GTGGGAGCTA TGAATCAGTA TAGAACAAGA 
ACTCTGAGTA TCACTTCTTC AGGCAGTGCT GTAAGCTGTT CAACAATTCC TCCAGAACTG 
GTGAAACAGA AGGTGAAACG TCAGTTGACA AAACAGCAAA AATCAGCTGT CAGACGTCGA 
TTGCAGAAAG GAGAAGCAAA TATATTTACC AAGCAACGTA GGGAAAACAT GCAAAATATC 
AAATCAAGTT TGGAAGCAGC CAGCTTTTGG GGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060813.2
CDS	57..1715
Translation	MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

Target ORF information:

RefSeq Version	NM_018343.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018343.3

ORF Insert Sequence:

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGAGA TGAAGAAAAT GTGGGAGCTA TGAATCAGTA TAGAACAAGA 
ACTCTGAGTA TCACTTCTTC AGGCAGTGCT GTAAGCTGTT CAACAATTCC TCCAGAACTG 
GTGAAACAGA AGGTGAAACG TCAGTTGACA AAACAGCAAA AATCAGCTGT CAGACGTCGA 
TTGCAGAAAG GAGAAGCAAA TATATTTACC AAGCAACGTA GGGAAAACAT GCAAAATATC 
AAATCAAGTT TGGAAGCAGC CAGCTTTTGG GGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK2 ( NM_018343.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060813.2 Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00091
Clone ID Related Accession (Same CDS sequence)	NM_018343.3 , NM_018343.2
Accession Version	NM_018343.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB240299.1, AK002021.1, AC008865.3 and CA415109.1. On May 1, 2009 this sequence version replaced NM_018343.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.219850.1, SRR1803617.101811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGAGA TGAAGAAAAT GTGGGAGCTA TGAATCAGTA TAGAACAAGA 
ACTCTGAGTA TCACTTCTTC AGGCAGTGCT GTAAGCTGTT CAACAATTCC TCCAGAACTG 
GTGAAACAGA AGGTGAAACG TCAGTTGACA AAACAGCAAA AATCAGCTGT CAGACGTCGA 
TTGCAGAAAG GAGAAGCAAA TATATTTACC AAGCAACGTA GGGAAAACAT GCAAAATATC 
AAATCAAGTT TGGAAGCAGC CAGCTTTTGG GGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060813.2
CDS	111..1769
Translation	MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

Target ORF information:

RefSeq Version	NM_018343.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018343.2

ORF Insert Sequence:

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGAGA TGAAGAAAAT GTGGGAGCTA TGAATCAGTA TAGAACAAGA 
ACTCTGAGTA TCACTTCTTC AGGCAGTGCT GTAAGCTGTT CAACAATTCC TCCAGAACTG 
GTGAAACAGA AGGTGAAACG TCAGTTGACA AAACAGCAAA AATCAGCTGT CAGACGTCGA 
TTGCAGAAAG GAGAAGCAAA TATATTTACC AAGCAACGTA GGGAAAACAT GCAAAATATC 
AAATCAAGTT TGGAAGCAGC CAGCTTTTGG GGAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK2 ( NM_001159749.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153221.1 Homo sapiens RIO kinase 2 (RIOK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03064
Clone ID Related Accession (Same CDS sequence)	NM_001159749.2 , NM_001159749.1
Accession Version	NM_001159749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB240299.1 and AK225348.1. On May 31, 2019 this sequence version replaced NM_001159749.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK225348.1, SRR1803611.57458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGGTA TAGACTTCTG TCGATTGCTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153221.1
CDS	57..1481
Translation	MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRYRLLSIAF

Target ORF information:

RefSeq Version	NM_001159749.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159749.2

ORF Insert Sequence:

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGGTA TAGACTTCTG TCGATTGCTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK2 ( NM_001159749.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153221.1 Homo sapiens RIO kinase 2 (RIOK2), transcript variant 2, mRNA.

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CloneID	OHu03064
Clone ID Related Accession (Same CDS sequence)	NM_001159749.2 , NM_001159749.1
Accession Version	NM_001159749.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB240299.1 and AK225348.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK225348.1, SRR1803611.57458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGGTA TAGACTTCTG TCGATTGCTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153221.1
CDS	111..1535
Translation	MGKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRYRLLSIAF

Target ORF information:

RefSeq Version	NM_001159749.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 2 (RIOK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159749.1

ORF Insert Sequence:

ATGGGGAAAG TGAATGTGGC CAAGTTGCGT TACATGAGCC GAGATGACTT CAGGGTCTTG 
ACCGCGGTTG AAATGGGCAT GAAGAACCAT GAAATTGTTC CCGGCAGTTT GATTGCTTCT 
ATAGCCAGCC TTAAACATGG TGGCTGTAAT AAAGTTTTAA GAGAATTAGT GAAACATAAA 
CTCATAGCTT GGGAGCGTAC CAAAACTGTC CAGGGCTATC GGTTGACAAA TGCAGGATAT 
GATTACCTAG CTTTGAAAAC ACTTTCTTCT AGGCAAGTAG TTGAGTCTGT TGGAAACCAG 
ATGGGTGTTG GCAAAGAATC AGATATTTAC ATTGTTGCAA ATGAAGAAGG ACAACAATTT 
GCATTAAAGC TTCACAGACT AGGAAGAACC TCGTTTCGAA ATTTGAAAAA CAAACGCGAT 
TATCATAAAC ATAGGCACAA TGTGTCATGG CTATATTTAT CTCGTCTCTC TGCCATGAAG 
GAATTTGCCT ATATGAAGGC ATTGTATGAG AGGAAATTTC CAGTTCCAAA GCCAATTGAT 
TACAATCGTC ATGCAGTGGT CATGGAACTC ATAAATGGTT ATCCACTATG TCAGATACAC 
CATGTTGAAG ATCCTGCATC AGTATATGAT GAAGCTATGG AACTAATTGT CAAACTTGCA 
AATCATGGGC TGATTCATGG AGATTTTAAT GAATTTAATC TCATTTTGGA TGAAAGTGAC 
CATATCACCA TGATTGATTT TCCACAGATG GTTTCAACTT CTCATCCCAA TGCTGAGTGG 
TATTTTGACA GAGATGTTAA ATGCATTAAA GATTTCTTTA TGAAACGTTT CAGCTACGAA 
AGTGAGCTTT TTCCAACTTT TAAGGATATC AGGAGAGAAG ACACTCTTGA TGTGGAGGTT 
TCTGCCAGTG GCTACACAAA GGAAATGCAG GCAGATGATG AACTGCTTCA TCCATTAGGT 
CCAGATGATA AAAATATTGA AACAAAAGAG GGATCTGAAT TCTCATTTTC AGATGGAGAA 
GTGGCAGAAA AAGCAGAGGT TTACGGGTCA GAAAATGAAA GTGAACGGAA CTGTCTAGAA 
GAATCAGAGG GCTGCTATTG CAGATCATCT GGAGACCCTG AACAAATAAA GGAAGACAGT 
TTATCAGAAG AGAGTGCTGA TGCACGGAGT TTTGAAATGA CTGAATTCAA TCAAGCTTTA 
GAAGAAATAA AAGGGCAGGT TGTTGAAAAC AACTCTGTAA CTGAATTTTC TGAGGAGAAA 
AACAGAACTG AAAATTACAA CAGGCAAGAT GGTCAGAGAG TTCAAGGAGG AGTCCCTGCT 
GGCTCTGACG AGTATGAAGA TGAATGCCCT CATCTAATTG CCTTGTCGTC ATTAAATAGA 
GAATTCAGGC CTTTCAGGTA TAGACTTCTG TCGATTGCTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIOK2 ( XM_017009628.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865117.1 Homo sapiens RIO kinase 2 (RIOK2), transcript variant X1, mRNA.

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CloneID	OHu95944
Clone ID Related Accession (Same CDS sequence)	XM_017009628.1
Accession Version	XM_017009628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase RIO2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTCA TAAATGGTTA TCCACTATGT CAGATACACC ATGTTGAAGA TCCTGCATCA 
GTATATGATG AAGCTATGGA ACTAATTGTC AAACTTGCAA ATCATGGGCT GATTCATGGA 
GATTTTAATG AATTTAATCT CATTTTGGAT GAAAGTGACC ATATCACCAT GATTGATTTT 
CCACAGATGG TTTCAACTTC TCATCCCAAT GCTGAGTGGT ATTTTGACAG AGATGTTAAA 
TGCATTAAAG ATTTCTTTAT GAAACGTTTC AGCTACGAAA GTGAGCTTTT TCCAACTTTT 
AAGGATATCA GGAGAGAAGA CACTCTTGAT GTGGAGGTTT CTGCCAGTGG CTACACAAAG 
GAAATGCAGG CAGATGATGA ACTGCTTCAT CCATTAGGTC CAGATGATAA AAATATTGAA 
ACAAAAGAGG GATCTGAATT CTCATTTTCA GATGGAGAAG TGGCAGAAAA AGCAGAGGTT 
TACGGGTCAG AAAATGAAAG TGAACGGAAC TGTCTAGAAG AATCAGAGGG CTGCTATTGC 
AGATCATCTG GAGACCCTGA ACAAATAAAG GAAGACAGTT TATCAGAAGA GAGTGCTGAT 
GCACGGAGTT TTGAAATGAC TGAATTCAAT CAAGCTTTAG AAGAAATAAA AGGGCAGGTT 
GTTGAAAACA ACTCTGTAAC TGAATTTTCT GAGGAGAAAA ACAGAACTGA AAATTACAAC 
AGGCAAGATG GTCAGAGAGT TCAAGGAGGA GTCCCTGCTG GCTCTGACGA GTATGAAGAT 
GAATGCCCTC ATCTAATTGC CTTGTCGTCA TTAAATAGAG AATTCAGGCC TTTCAGAGAT 
GAAGAAAATG TGGGAGCTAT GAATCAGTAT AGAACAAGAA CTCTGAGTAT CACTTCTTCA 
GGCAGTGCTG TAAGCTGTTC AACAATTCCT CCAGAACTGG TGAAACAGAA GGTGAAACGT 
CAGTTGACAA AACAGCAAAA ATCAGCTGTC AGACGTCGAT TGCAGAAAGG AGAAGCAAAT 
ATATTTACCA AGCAACGTAG GGAAAACATG CAAAATATCA AATCAAGTTT GGAAGCAGCC 
AGCTTTTGGG GAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865117.1
CDS	297..1394
Translation	MELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

Target ORF information:

RefSeq Version	XM_017009628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RIO kinase 2 (RIOK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009628.1

ORF Insert Sequence:

ATGGAACTCA TAAATGGTTA TCCACTATGT CAGATACACC ATGTTGAAGA TCCTGCATCA 
GTATATGATG AAGCTATGGA ACTAATTGTC AAACTTGCAA ATCATGGGCT GATTCATGGA 
GATTTTAATG AATTTAATCT CATTTTGGAT GAAAGTGACC ATATCACCAT GATTGATTTT 
CCACAGATGG TTTCAACTTC TCATCCCAAT GCTGAGTGGT ATTTTGACAG AGATGTTAAA 
TGCATTAAAG ATTTCTTTAT GAAACGTTTC AGCTACGAAA GTGAGCTTTT TCCAACTTTT 
AAGGATATCA GGAGAGAAGA CACTCTTGAT GTGGAGGTTT CTGCCAGTGG CTACACAAAG 
GAAATGCAGG CAGATGATGA ACTGCTTCAT CCATTAGGTC CAGATGATAA AAATATTGAA 
ACAAAAGAGG GATCTGAATT CTCATTTTCA GATGGAGAAG TGGCAGAAAA AGCAGAGGTT 
TACGGGTCAG AAAATGAAAG TGAACGGAAC TGTCTAGAAG AATCAGAGGG CTGCTATTGC 
AGATCATCTG GAGACCCTGA ACAAATAAAG GAAGACAGTT TATCAGAAGA GAGTGCTGAT 
GCACGGAGTT TTGAAATGAC TGAATTCAAT CAAGCTTTAG AAGAAATAAA AGGGCAGGTT 
GTTGAAAACA ACTCTGTAAC TGAATTTTCT GAGGAGAAAA ACAGAACTGA AAATTACAAC 
AGGCAAGATG GTCAGAGAGT TCAAGGAGGA GTCCCTGCTG GCTCTGACGA GTATGAAGAT 
GAATGCCCTC ATCTAATTGC CTTGTCGTCA TTAAATAGAG AATTCAGGCC TTTCAGAGAT 
GAAGAAAATG TGGGAGCTAT GAATCAGTAT AGAACAAGAA CTCTGAGTAT CACTTCTTCA 
GGCAGTGCTG TAAGCTGTTC AACAATTCCT CCAGAACTGG TGAAACAGAA GGTGAAACGT 
CAGTTGACAA AACAGCAAAA ATCAGCTGTC AGACGTCGAT TGCAGAAAGG AGAAGCAAAT 
ATATTTACCA AGCAACGTAG GGAAAACATG CAAAATATCA AATCAAGTTT GGAAGCAGCC 
AGCTTTTGGG GAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High Expression of RIOK2 and NOB1 Predict Human Non-small Cell Lung Cancer Outcomes.
Scientific reports628666(2016 Jun)
Liu K,Chen HL,Wang S,Gu MM,Chen XM,Zhang SL,Yu KJ,You QS

Discovery of selective RIO2 kinase small molecule ligand.
Biochimica et biophysica acta1854(10 Pt B)1630-6(2015 Oct)
Varin T,Godfrey AG,Masquelin T,Nicolaou CA,Evans DA,Vieth M

A kinome-wide RNAi screen in Drosophila Glia reveals that the RIO kinases mediate cell proliferation and survival through TORC2-Akt signaling in glioblastoma.
PLoS genetics9(2)e1003253(2013)
Read RD,Fenton TR,Gomez GG,Wykosky J,Vandenberg SR,Babic I,Iwanami A,Yang H,Cavenee WK,Mischel PS,Furnari FB,Thomas JB

Phosphorylation of right open reading frame 2 (Rio2) protein kinase by polo-like kinase 1 regulates mitotic progression.
The Journal of biological chemistry286(42)36352-60(2011 Oct)
Liu T,Deng M,Li J,Tong X,Wei Q,Ye X

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RIOK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム