PRKAR2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAR2B
Entrez Gene ID	5577
Full Name	protein kinase cAMP-dependent type II regulatory subunit beta
Synonyms	PRKAR2,RII-BETA
General protein information	Preferred Names protein kinase cAMP-dependent type II regulatory subunit beta Names cAMP-dependent protein kinase type II-beta regulatory subunit H_RG363E19.2 WUGSC:H_RG363E19.2 cAMP-dependent protein kinase type II-beta regulatory chain protein kinase, cAMP-dependent, regulatory subunit type II beta protein kinase, cAMP-dependent, regulatory, type II, beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.3
Summary	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq, Jul 2008].
Disorder MIM:	176912

mRNA and Protein(s)

mRNA	Protein	Name
XM_011516398.1	XP_011514700.1	cAMP-dependent protein kinase type II-beta regulatory subunit isoform X1
NM_002736.2	NP_002727.2	cAMP-dependent protein kinase type II-beta regulatory subunit
XM_024446836.1	XP_024302604.1	cAMP-dependent protein kinase type II-beta regulatory subunit isoform X2
NM_002736.3	NP_002727.2	cAMP-dependent protein kinase type II-beta regulatory subunit

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkar2b	NP_035288.2
Bos taurus (cattle)	PRKAR2B	NP_777074.1
Rattus norvegicus (Norway rat)	Prkar2b	NP_001025191.1
Xenopus tropicalis (tropical clawed frog)	prkar2b	XP_002933139.2
Homo sapiens (human)	PRKAR2B	NP_002727.2
	PRKAR2B	XP_001089109.2
Gallus gallus (chicken)	PRKAR2B	XP_001232697.3
Pan troglodytes (chimpanzee)	PRKAR2B	XP_001148361.2
Danio rerio (zebrafish)	LOC100331387	XP_002666815.3
Canis lupus familiaris (dog)	PRKAR2B	XP_858066.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Differential expression of the protein kinase A subunits in normal adrenal glands and adrenocortical adenomas.
Weigand I, Ronchi CL, Rizk-Rabin M, Dalmazi GD, Wild V, Bathon K, Rubin B, Calebiro D, Beuschlein F, Bertherat J, Fassnacht M, Sbiera S
Scientific reports7(1)49(2017 03)

PRKAR2B plays an oncogenic role in the castration-resistant prostate cancer.
Sha J, Xue W, Dong B, Pan J, Wu X, Li D, Liu D, Huang Y
Oncotarget8(4)6114-6129(2017 Jan)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Release of pRIIbeta in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site inhibits holoenzyme reassociation with the catalytic subunit.
Title: Single Turnover Autophosphorylation Cycle of the PKA RII? Holoenzyme.

Leu206Arg and Leu199_Cys200insTrp mutations in PRKACA impair its association with PRKAR2B and PRKAR1A.
Title: PKA catalytic subunit mutations in adrenocortical Cushing's adenoma impair association with the regulatory subunit.

Although the depletion of PRKAR1A and PRKAR2B in adrenocortical cells has similar effects on cell proliferation and apoptosis; loss of these PKA subunits differentially affects cyclin expression.
Title: Comparison of the effects of PRKAR1A and PRKAR2B depletion on signaling pathways, cell growth, and cell cycle control of adrenocortical cells.

Because of limited power, PRKAR2B's role in antipsychotic-induced weight gain is unclear, but biological evidence suggests that PRKAR2B may be involved
Title: Protein kinase cAMP-dependent regulatory type II beta (PRKAR2B) gene variants in antipsychotic-induced weight gain.

Meta-analysis and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Meta-analysis of genome-wide association studies confirms a susceptibility locus for knee osteoarthritis on chromosome 7q22.

The following PRKAR2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAR2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03971	NM_002736.3 Latest version!	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu59905	XM_011516398.1 Latest version!	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu59906	XM_024446836.1 Latest version!	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu03971	NM_002736.2	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAR2B ( NM_002736.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002727.2 Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Price & Availability↑

CloneID	OHu03971
Clone ID Related Accession (Same CDS sequence)	NM_002736.2 , NM_002736.3
Accession Version	NM_002736.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF593944.1, AL559918.3, M31158.1, CA405423.1, AK128639.1 and BQ022817.1. On May 12, 2004 this sequence version replaced NM_002736.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075800.1, SRR1660809.100739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCATCG AGATCCCGGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC CCGCCTGCAG 
CAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
CTGGGCGCCG CTGCCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
ATGCAGTCCG ACTCCGAGGA CGGGGAGGAG GAGGAGGCGG CGCCCGCGGA CGCAGGGGCG 
TTCAATGCTC CAGTAATAAA CCGATTCACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCAGAG TCCAGGATTA TACATCCAAA AACTGATGAT 
CAAAGAAATA GGTTGCAAGA GGCTTGCAAA GACATCCTGC TGTTTAAGAA TCTGGATCCG 
GAGCAGATGT CTCAAGTATT AGATGCCATG TTTGAAAAAT TGGTCAAAGA TGGGGAGCAT 
GTAATTGATC AAGGTGACGA TGGTGACAAC TTTTATGTAA TTGATAGAGG CACATTTGAT 
ATTTATGTGA AATGTGATGG TGTTGGAAGA TGTGTTGGTA ACTATGATAA TCGTGGGAGT 
TTCGGCGAAC TGGCCTTAAT GTACAATACA CCCAGAGCAG CTACAATCAC TGCTACCTCT 
CCTGGTGCTC TGTGGGGTTT GGACAGGGTA ACCTTCAGGA GAATAATTGT GAAAAACAAT 
GCCAAAAAGA GAAAAATGTA TGAAAGCTTT ATTGAGTCAC TGCCATTCCT TAAATCTTTG 
GAGTTTTCTG AACGCCTGAA AGTAGTAGAT GTGATAGGCA CCAAAGTATA CAACGATGGA 
GAACAAATCA TTGCTCAGGG AGATTCGGCT GATTCTTTTT TCATTGTAGA ATCTGGAGAA 
GTGAAAATTA CTATGAAAAG AAAGGGTAAA TCAGAAGTGG AAGAGAATGG TGCAGTAGAA 
ATCGCTCGAT GCTCGCGGGG ACAGTACTTT GGAGAGCTTG CCCTGGTAAC TAACAAACCT 
CGAGCAGCTT CTGCCCACGC CATTGGGACT GTCAAATGTT TAGCAATGGA TGTGCAAGCA 
TTTGAAAGGC TTCTGGGACC TTGCATGGAA ATTATGAAAA GGAACATCGC TACCTATGAA 
GAACAGTTAG TTGCCCTGTT TGGAACGAAC ATGGATATTG TTGAACCCAC TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002727.2
CDS	176..1432
Translation	MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFGHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	NM_002736.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002736.2

ORF Insert Sequence:

ATGAGCATCG AGATCCCGGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC CCGCCTGCAG 
CAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
CTGGGCGCCG CTGCCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
ATGCAGTCCG ACTCCGAGGA CGGGGAGGAG GAGGAGGCGG CGCCCGCGGA CGCAGGGGCG 
TTCAATGCTC CAGTAATAAA CCGATTCACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCAGAG TCCAGGATTA TACATCCAAA AACTGATGAT 
CAAAGAAATA GGTTGCAAGA GGCTTGCAAA GACATCCTGC TGTTTAAGAA TCTGGATCCG 
GAGCAGATGT CTCAAGTATT AGATGCCATG TTTGAAAAAT TGGTCAAAGA TGGGGAGCAT 
GTAATTGATC AAGGTGACGA TGGTGACAAC TTTTATGTAA TTGATAGAGG CACATTTGAT 
ATTTATGTGA AATGTGATGG TGTTGGAAGA TGTGTTGGTA ACTATGATAA TCGTGGGAGT 
TTCGGCGAAC TGGCCTTAAT GTACAATACA CCCAGAGCAG CTACAATCAC TGCTACCTCT 
CCTGGTGCTC TGTGGGGTTT GGACAGGGTA ACCTTCAGGA GAATAATTGT GAAAAACAAT 
GCCAAAAAGA GAAAAATGTA TGAAAGCTTT ATTGAGTCAC TGCCATTCCT TAAATCTTTG 
GAGTTTTCTG AACGCCTGAA AGTAGTAGAT GTGATAGGCA CCAAAGTATA CAACGATGGA 
GAACAAATCA TTGCTCAGGG AGATTCGGCT GATTCTTTTT TCATTGTAGA ATCTGGAGAA 
GTGAAAATTA CTATGAAAAG AAAGGGTAAA TCAGAAGTGG AAGAGAATGG TGCAGTAGAA 
ATCGCTCGAT GCTCGCGGGG ACAGTACTTT GGAGAGCTTG CCCTGGTAAC TAACAAACCT 
CGAGCAGCTT CTGCCCACGC CATTGGGACT GTCAAATGTT TAGCAATGGA TGTGCAAGCA 
TTTGAAAGGC TTCTGGGACC TTGCATGGAA ATTATGAAAA GGAACATCGC TACCTATGAA 
GAACAGTTAG TTGCCCTGTT TGGAACGAAC ATGGATATTG TTGAACCCAC TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR2B ( NM_002736.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002727.2 Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Price & Availability↑

CloneID	OHu03971
Clone ID Related Accession (Same CDS sequence)	NM_002736.2 , NM_002736.3
Accession Version	NM_002736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006387.3, CF593944.1, AL559918.3, M31158.1, CA405423.1, AK128639.1 and BQ022817.1. On Nov 23, 2018 this sequence version replaced NM_002736.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075800.1, SRR1660809.100739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265717.5/ ENSP00000265717.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCATCG AGATCCCGGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC CCGCCTGCAG 
CAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
CTGGGCGCCG CTGCCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
ATGCAGTCCG ACTCCGAGGA CGGGGAGGAG GAGGAGGCGG CGCCCGCGGA CGCAGGGGCG 
TTCAATGCTC CAGTAATAAA CCGATTCACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCAGAG TCCAGGATTA TACATCCAAA AACTGATGAT 
CAAAGAAATA GGTTGCAAGA GGCTTGCAAA GACATCCTGC TGTTTAAGAA TCTGGATCCG 
GAGCAGATGT CTCAAGTATT AGATGCCATG TTTGAAAAAT TGGTCAAAGA TGGGGAGCAT 
GTAATTGATC AAGGTGACGA TGGTGACAAC TTTTATGTAA TTGATAGAGG CACATTTGAT 
ATTTATGTGA AATGTGATGG TGTTGGAAGA TGTGTTGGTA ACTATGATAA TCGTGGGAGT 
TTCGGCGAAC TGGCCTTAAT GTACAATACA CCCAGAGCAG CTACAATCAC TGCTACCTCT 
CCTGGTGCTC TGTGGGGTTT GGACAGGGTA ACCTTCAGGA GAATAATTGT GAAAAACAAT 
GCCAAAAAGA GAAAAATGTA TGAAAGCTTT ATTGAGTCAC TGCCATTCCT TAAATCTTTG 
GAGTTTTCTG AACGCCTGAA AGTAGTAGAT GTGATAGGCA CCAAAGTATA CAACGATGGA 
GAACAAATCA TTGCTCAGGG AGATTCGGCT GATTCTTTTT TCATTGTAGA ATCTGGAGAA 
GTGAAAATTA CTATGAAAAG AAAGGGTAAA TCAGAAGTGG AAGAGAATGG TGCAGTAGAA 
ATCGCTCGAT GCTCGCGGGG ACAGTACTTT GGAGAGCTTG CCCTGGTAAC TAACAAACCT 
CGAGCAGCTT CTGCCCACGC CATTGGGACT GTCAAATGTT TAGCAATGGA TGTGCAAGCA 
TTTGAAAGGC TTCTGGGACC TTGCATGGAA ATTATGAAAA GGAACATCGC TACCTATGAA 
GAACAGTTAG TTGCCCTGTT TGGAACGAAC ATGGATATTG TTGAACCCAC TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002727.2
CDS	204..1460
Translation	MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFGHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	NM_002736.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002736.3

ORF Insert Sequence:

ATGAGCATCG AGATCCCGGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC CCGCCTGCAG 
CAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
CTGGGCGCCG CTGCCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
ATGCAGTCCG ACTCCGAGGA CGGGGAGGAG GAGGAGGCGG CGCCCGCGGA CGCAGGGGCG 
TTCAATGCTC CAGTAATAAA CCGATTCACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCAGAG TCCAGGATTA TACATCCAAA AACTGATGAT 
CAAAGAAATA GGTTGCAAGA GGCTTGCAAA GACATCCTGC TGTTTAAGAA TCTGGATCCG 
GAGCAGATGT CTCAAGTATT AGATGCCATG TTTGAAAAAT TGGTCAAAGA TGGGGAGCAT 
GTAATTGATC AAGGTGACGA TGGTGACAAC TTTTATGTAA TTGATAGAGG CACATTTGAT 
ATTTATGTGA AATGTGATGG TGTTGGAAGA TGTGTTGGTA ACTATGATAA TCGTGGGAGT 
TTCGGCGAAC TGGCCTTAAT GTACAATACA CCCAGAGCAG CTACAATCAC TGCTACCTCT 
CCTGGTGCTC TGTGGGGTTT GGACAGGGTA ACCTTCAGGA GAATAATTGT GAAAAACAAT 
GCCAAAAAGA GAAAAATGTA TGAAAGCTTT ATTGAGTCAC TGCCATTCCT TAAATCTTTG 
GAGTTTTCTG AACGCCTGAA AGTAGTAGAT GTGATAGGCA CCAAAGTATA CAACGATGGA 
GAACAAATCA TTGCTCAGGG AGATTCGGCT GATTCTTTTT TCATTGTAGA ATCTGGAGAA 
GTGAAAATTA CTATGAAAAG AAAGGGTAAA TCAGAAGTGG AAGAGAATGG TGCAGTAGAA 
ATCGCTCGAT GCTCGCGGGG ACAGTACTTT GGAGAGCTTG CCCTGGTAAC TAACAAACCT 
CGAGCAGCTT CTGCCCACGC CATTGGGACT GTCAAATGTT TAGCAATGGA TGTGCAAGCA 
TTTGAAAGGC TTCTGGGACC TTGCATGGAA ATTATGAAAA GGAACATCGC TACCTATGAA 
GAACAGTTAG TTGCCCTGTT TGGAACGAAC ATGGATATTG TTGAACCCAC TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR2B ( XM_011516398.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514700.1 Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59905
Clone ID Related Accession (Same CDS sequence)	XM_011516398.1
Accession Version	XM_011516398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCG AGATCCCGGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC CCGCCTGCAG 
CAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
CTGGGCGCCG CTGCCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
ATGCAGTCCG ACTCCGAGGA CGGGGAGGAG GAGGAGGCGG CGCCCGCGGA CGCAGGGGCG 
TTCAATGCTC CAGTAATAAA CCGATTCACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCAGAG TCCAGGGAGC AGATGTCTCA AGTATTAGAT 
GCCATGTTTG AAAAATTGGT CAAAGATGGG GAGCATGTAA TTGATCAAGG TGACGATGGT 
GACAACTTTT ATGTAATTGA TAGAGGCACA TTTGATATTT ATGTGAAATG TGATGGTGTT 
GGAAGATGTG TTGGTAACTA TGATAATCGT GGGAGTTTCG GCGAACTGGC CTTAATGTAC 
AATACACCCA GAGCAGCTAC AATCACTGCT ACCTCTCCTG GTGCTCTGTG GGGTTTGGAC 
AGGGTAACCT TCAGGAGAAT AATTGTGAAA AACAATGCCA AAAAGAGAAA AATGTATGAA 
AGCTTTATTG AGTCACTGCC ATTCCTTAAA TCTTTGGAGT TTTCTGAACG CCTGAAAGTA 
GTAGATGTGA TAGGCACCAA AGTATACAAC GATGGAGAAC AAATCATTGC TCAGGGAGAT 
TCGGCTGATT CTTTTTTCAT TGTAGAATCT GGAGAAGTGA AAATTACTAT GAAAAGAAAG 
GGTAAATCAG AAGTGGAAGA GAATGGTGCA GTAGAAATCG CTCGATGCTC GCGGGGACAG 
TACTTTGGAG AGCTTGCCCT GGTAACTAAC AAACCTCGAG CAGCTTCTGC CCACGCCATT 
GGGACTGTCA AATGTTTAGC AATGGATGTG CAAGCATTTG AAAGGCTTCT GGGACCTTGC 
ATGGAAATTA TGAAAAGGAA CATCGCTACC TATGAAGAAC AGTTAGTTGC CCTGTTTGGA 
ACGAACATGG ATATTGTTGA ACCCACTGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514700.1
CDS	181..1353
Translation	MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFGHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESREQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	XM_011516398.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516398.1

ORF Insert Sequence:

ATGAGCATCG AGATCCCGGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC GGTGGAGGTG 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC CCGCCTGCAG 
CAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AGGGCAGGAC CTGGGGGGAC 
CTGGGCGCCG CTGCCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
ATGCAGTCCG ACTCCGAGGA CGGGGAGGAG GAGGAGGCGG CGCCCGCGGA CGCAGGGGCG 
TTCAATGCTC CAGTAATAAA CCGATTCACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCAGAG TCCAGGGAGC AGATGTCTCA AGTATTAGAT 
GCCATGTTTG AAAAATTGGT CAAAGATGGG GAGCATGTAA TTGATCAAGG TGACGATGGT 
GACAACTTTT ATGTAATTGA TAGAGGCACA TTTGATATTT ATGTGAAATG TGATGGTGTT 
GGAAGATGTG TTGGTAACTA TGATAATCGT GGGAGTTTCG GCGAACTGGC CTTAATGTAC 
AATACACCCA GAGCAGCTAC AATCACTGCT ACCTCTCCTG GTGCTCTGTG GGGTTTGGAC 
AGGGTAACCT TCAGGAGAAT AATTGTGAAA AACAATGCCA AAAAGAGAAA AATGTATGAA 
AGCTTTATTG AGTCACTGCC ATTCCTTAAA TCTTTGGAGT TTTCTGAACG CCTGAAAGTA 
GTAGATGTGA TAGGCACCAA AGTATACAAC GATGGAGAAC AAATCATTGC TCAGGGAGAT 
TCGGCTGATT CTTTTTTCAT TGTAGAATCT GGAGAAGTGA AAATTACTAT GAAAAGAAAG 
GGTAAATCAG AAGTGGAAGA GAATGGTGCA GTAGAAATCG CTCGATGCTC GCGGGGACAG 
TACTTTGGAG AGCTTGCCCT GGTAACTAAC AAACCTCGAG CAGCTTCTGC CCACGCCATT 
GGGACTGTCA AATGTTTAGC AATGGATGTG CAAGCATTTG AAAGGCTTCT GGGACCTTGC 
ATGGAAATTA TGAAAAGGAA CATCGCTACC TATGAAGAAC AGTTAGTTGC CCTGTTTGGA 
ACGAACATGG ATATTGTTGA ACCCACTGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR2B ( XM_024446836.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302604.1 Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), transcript variant X2, mRNA.

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CloneID	OHu59906
Clone ID Related Accession (Same CDS sequence)	XM_024446836.1
Accession Version	XM_024446836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAG TATTAGATGC CATGTTTGAA AAATTGGTCA AAGATGGGGA GCATGTAATT 
GATCAAGGTG ACGATGGTGA CAACTTTTAT GTAATTGATA GAGGCACATT TGATATTTAT 
GTGAAATGTG ATGGTGTTGG AAGATGTGTT GGTAACTATG ATAATCGTGG GAGTTTCGGC 
GAACTGGCCT TAATGTACAA TACACCCAGA GCAGCTACAA TCACTGCTAC CTCTCCTGGT 
GCTCTGTGGG GTTTGGACAG GGTAACCTTC AGGAGAATAA TTGTGAAAAA CAATGCCAAA 
AAGAGAAAAA TGTATGAAAG CTTTATTGAG TCACTGCCAT TCCTTAAATC TTTGGAGTTT 
TCTGAACGCC TGAAAGTAGT AGATGTGATA GGCACCAAAG TATACAACGA TGGAGAACAA 
ATCATTGCTC AGGGAGATTC GGCTGATTCT TTTTTCATTG TAGAATCTGG AGAAGTGAAA 
ATTACTATGA AAAGAAAGGG TAAATCAGAA GTGGAAGAGA ATGGTGCAGT AGAAATCGCT 
CGATGCTCGC GGGGACAGTA CTTTGGAGAG CTTGCCCTGG TAACTAACAA ACCTCGAGCA 
GCTTCTGCCC ACGCCATTGG GACTGTCAAA TGTTTAGCAA TGGATGTGCA AGCATTTGAA 
AGGCTTCTGG GACCTTGCAT GGAAATTATG AAAAGGAACA TCGCTACCTA TGAAGAACAG 
TTAGTTGCCC TGTTTGGAAC GAACATGGAT ATTGTTGAAC CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302604.1
CDS	457..1227
Translation	MSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	XM_024446836.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446836.1

ORF Insert Sequence:

ATGTCTCAAG TATTAGATGC CATGTTTGAA AAATTGGTCA AAGATGGGGA GCATGTAATT 
GATCAAGGTG ACGATGGTGA CAACTTTTAT GTAATTGATA GAGGCACATT TGATATTTAT 
GTGAAATGTG ATGGTGTTGG AAGATGTGTT GGTAACTATG ATAATCGTGG GAGTTTCGGC 
GAACTGGCCT TAATGTACAA TACACCCAGA GCAGCTACAA TCACTGCTAC CTCTCCTGGT 
GCTCTGTGGG GTTTGGACAG GGTAACCTTC AGGAGAATAA TTGTGAAAAA CAATGCCAAA 
AAGAGAAAAA TGTATGAAAG CTTTATTGAG TCACTGCCAT TCCTTAAATC TTTGGAGTTT 
TCTGAACGCC TGAAAGTAGT AGATGTGATA GGCACCAAAG TATACAACGA TGGAGAACAA 
ATCATTGCTC AGGGAGATTC GGCTGATTCT TTTTTCATTG TAGAATCTGG AGAAGTGAAA 
ATTACTATGA AAAGAAAGGG TAAATCAGAA GTGGAAGAGA ATGGTGCAGT AGAAATCGCT 
CGATGCTCGC GGGGACAGTA CTTTGGAGAG CTTGCCCTGG TAACTAACAA ACCTCGAGCA 
GCTTCTGCCC ACGCCATTGG GACTGTCAAA TGTTTAGCAA TGGATGTGCA AGCATTTGAA 
AGGCTTCTGG GACCTTGCAT GGAAATTATG AAAAGGAACA TCGCTACCTA TGAAGAACAG 
TTAGTTGCCC TGTTTGGAAC GAACATGGAT ATTGTTGAAC CCACTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Differential expression of the protein kinase A subunits in normal adrenal glands and adrenocortical adenomas.
Scientific reports7(1)49(2017 03)
Weigand I,Ronchi CL,Rizk-Rabin M,Dalmazi GD,Wild V,Bathon K,Rubin B,Calebiro D,Beuschlein F,Bertherat J,Fassnacht M,Sbiera S

PRKAR2B plays an oncogenic role in the castration-resistant prostate cancer.
Oncotarget8(4)6114-6129(2017 Jan)
Sha J,Xue W,Dong B,Pan J,Wu X,Li D,Liu D,Huang Y

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

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PRKAR2B cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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