DHX32 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX32
Entrez Gene ID	55760
Full Name	DEAH-box helicase 32 (putative)
Synonyms	DDX32,DHLP1
General protein information	Preferred Names DEAH-box helicase 32 (putative) Names putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32 DEAD/H box 32 DEAD/H helicase-like protein-1 DEAH (Asp-Glu-Ala-His) box polypeptide 32 DEAH box protein 32 huDDX32
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q26.2
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. [provided by RefSeq, Jul 2008].
Disorder MIM:	607960

mRNA and Protein(s)

mRNA	Protein	Name
NM_018180.3	NP_060650.2	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
NM_018180.2	NP_060650.2	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
XM_017016404.1	XP_016871893.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
XM_017016405.1	XP_016871894.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1

Pathways from BioSystems

Homologies

	DHX32	XP_001085971.1
Bos taurus (cattle)	DHX32	NP_001094614.1
Xenopus tropicalis (tropical clawed frog)	dhx32	XP_002932696.2
Canis lupus familiaris (dog)	DHX32	XP_003433686.1
Gallus gallus (chicken)	LOC768812	XP_003641552.1
Danio rerio (zebrafish)	dhx32	XP_005158794.1
Danio rerio (zebrafish)	dhx32a	NP_001189401.1
Homo sapiens (human)	DHX32	NP_060650.2
Mus musculus (house mouse)	Dhx32	NP_001272960.1
Rattus norvegicus (Norway rat)	Dhx32	NP_001123511.1
Pan troglodytes (chimpanzee)	DHX32	XP_001138928.1

Gene Ontology
Bibliography

Related articles in PubMed

DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting ?-catenin Signaling to Induce Expression of VEGFA.
Lin H, Fang Z, Su Y, Li P, Wang J, Liao H, Hu Q, Ye C, Fang Y, Luo Q, Lin Z, Pan C, Wang F, Zhang ZY
EBioMedicine1862-72(2017 Apr)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer.
Huang C, Liang X, Huang R, Zhang Z
Journal of experimental & clinical cancer research : CR2811(2009 Jan)

Altered distribution of heat shock protein 60 (Hsp60) with dysregulated expression of DHX32.
Chen Y, Alli Z, Ackerley C, Al-Saud B, Abdelhaleem M
Experimental and molecular pathology82(3)256-61(2007 Jun)

A role for DHX32 in regulating T-cell apoptosis.
Alli Z, Chen Y, Abdul Wajid S, Al-Saud B, Abdelhaleem M
Anticancer research27(1A)373-7(2007 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DHX32 is an upstream regulator for beta-catenin, which promotes the activity of beta-catenin to upregulate expression of VEGFA in colorectal cancer cells. Depletion of DHX32 expression suppressed colorectal cancer tumor angiogenesis and tumor growth.
Title: DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting ?-catenin Signaling to Induce Expression of VEGFA.

DHX32 may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation.
Title: Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer.

dysregulated expression of DHX32 might lead to as of yet unknown changes in mitochondrial homeostasis manifested by cytoplasmic redistribution of the molecular chaperon Hsp60
Title: Altered distribution of heat shock protein 60 (Hsp60) with dysregulated expression of DHX32.

In peripheral blood lymphocytes, a correlation between DHX32 and c-FLIP short expression was detected in response to different T-cell activation stimuli. Results suggest DHX32 involvement in regulating T-cell response to apoptotic stimuli.
Title: A role for DHX32 in regulating T-cell apoptosis.

These results suggest that DHX32 is a putative RNA helicase that might be involved in regulating nuclear and mitochondrial gene expression.
Title: Nuclear and mitochondrial localization of the putative RNA helicase DHX32.

The following DHX32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02952	NM_018180.3 Latest version!	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu02952	XM_017016404.1 Latest version!	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu02952	XM_017016405.1 Latest version!	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu02952	NM_018180.2	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX32 ( NM_018180.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060650.2 Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.

Price & Availability↑

CloneID	OHu02952
Clone ID Related Accession (Same CDS sequence)	NM_018180.3 , NM_018180.2 , XM_017016404.1 , XM_017016405.1
Accession Version	NM_018180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL360176.22. On May 17, 2019 this sequence version replaced NM_018180.2. ##Evidence-Data-START## Transcript exon combination :: BC002473.2, SRR1660809.15622.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000284690.4/ ENSP00000284690.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060650.2
CDS	469..2700
Translation	MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFDGLPYSSRYYKLLKEREDLPIWKEKYSFMENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSSPHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIVVFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSNSVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQILGSSSSGKFFCLYTEEFASKDMTPLKPAEMQEANLTSMVLFMKRIDIAGLGHCDFMNRPAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEVLTIAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLNSSSEYCVEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGYFMQIARDVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITSEISPELFMQLVPQYYFSNLPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRCTLQ

Target ORF information:

RefSeq Version	NM_018180.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018180.3

ORF Insert Sequence:

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX32 ( NM_018180.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060650.2 Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.

Price & Availability↑

CloneID	OHu02952
Clone ID Related Accession (Same CDS sequence)	NM_018180.3 , NM_018180.2 , XM_017016404.1 , XM_017016405.1
Accession Version	NM_018180.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF427340.1. On Apr 28, 2002 this sequence version replaced NM_018180.1. ##Evidence-Data-START## Transcript exon combination :: AF427340.1, SRR1803613.262652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060650.2
CDS	492..2723
Translation	MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFDGLPYSSRYYKLLKEREDLPIWKEKYSFMENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSSPHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIVVFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSNSVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQILGSSSSGKFFCLYTEEFASKDMTPLKPAEMQEANLTSMVLFMKRIDIAGLGHCDFMNRPAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEVLTIAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLNSSSEYCVEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGYFMQIARDVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITSEISPELFMQLVPQYYFSNLPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRCTLQ

Target ORF information:

RefSeq Version	NM_018180.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018180.2

ORF Insert Sequence:

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX32 ( XM_017016404.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871893.1 Homo sapiens DEAH-box helicase 32 (putative) (DHX32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu02952
Clone ID Related Accession (Same CDS sequence)	NM_018180.3 , NM_018180.2 , XM_017016404.1 , XM_017016405.1
Accession Version	XM_017016404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871893.1
CDS	824..3055
Translation	MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFDGLPYSSRYYKLLKEREDLPIWKEKYSFMENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSSPHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIVVFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSNSVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQILGSSSSGKFFCLYTEEFASKDMTPLKPAEMQEANLTSMVLFMKRIDIAGLGHCDFMNRPAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEVLTIAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLNSSSEYCVEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGYFMQIARDVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITSEISPELFMQLVPQYYFSNLPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRCTLQ

Target ORF information:

RefSeq Version	XM_017016404.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016404.1

ORF Insert Sequence:

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX32 ( XM_017016405.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871894.1 Homo sapiens DEAH-box helicase 32 (putative) (DHX32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu02952
Clone ID Related Accession (Same CDS sequence)	NM_018180.3 , NM_018180.2 , XM_017016404.1 , XM_017016405.1
Accession Version	XM_017016405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871894.1
CDS	514..2745
Translation	MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFDGLPYSSRYYKLLKEREDLPIWKEKYSFMENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSSPHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIVVFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSNSVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQILGSSSSGKFFCLYTEEFASKDMTPLKPAEMQEANLTSMVLFMKRIDIAGLGHCDFMNRPAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEVLTIAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLNSSSEYCVEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGYFMQIARDVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITSEISPELFMQLVPQYYFSNLPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRCTLQ

Target ORF information:

RefSeq Version	XM_017016405.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAH-box helicase 32 (putative) (DHX32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016405.1

ORF Insert Sequence:

ATGGAAGAAG AAGGGCTGGA GTGTCCAAAC TCTTCCTCTG AAAAACGCTA TTTTCCTGAA 
TCCCTGGATT CCAGCGATGG GGATGAGGAA GAGGTTTTGG CCTGTGAGGA TTTGGAACTT 
AACCCCTTTG ATGGATTGCC ATATTCATCA CGTTATTATA AACTTCTGAA AGAAAGAGAA 
GATCTTCCTA TATGGAAAGA AAAATACTCC TTTATGGAGA ACCTGCTTCA AAATCAAATC 
GTGATTGTTT CAGGAGATGC TAAATGTGGT AAGAGCGCTC AGGTTCCTCA GTGGTGTGCT 
GAATATTGTC TTTCCATCCA CTACCAGCAC GGGGGCGTGA TATGCACACA GGTCCACAAG 
CAGACTGTGG TCCAGCTCGC CCTGCGGGTG GCGGATGAAA TGGATGTTAA CATTGGTCAT 
GAGGTTGGCT ACGTGATCCC TTTCGAGAAC TGCTGTACCA ACGAAACAAT CCTGAGGTAT 
TGTACTGATG ATATGCTGCA AAGAGAAATG ATGTCCAATC CTTTTTTGGG TAGCTATGGG 
GTCATCATCT TAGATGATAT TCATGAAAGA AGCATTGCAA CTGATGTGTT ACTTGGACTT 
CTTAAAGATG TTTTACTAGC AAGACCAGAA CTGAAGCTCA TAATTAACTC CTCACCTCAC 
CTGATCAGCA AACTCAATTC TTATTATGGA AACGTGCCTG TCATAGAAGT GAAAAATAAA 
CACCCTGTGG AGGTTGTGTA CCTTAGTGAG GCTCAAAAGG ATTCTTTTGA GTCTATTTTA 
CGCCTTATCT TTGAAATTCA CCACTCGGGT GAGAAAGGTG ACATTGTAGT CTTTCTGGCC 
TGTGAACAAG ATATTGAGAA AGTCTGTGAA ACTGTCTATC AAGGATCTAA CCTAAACCCA 
GATCTTGGAG AACTGGTGGT TGTTCCTTTG TATCCAAAAG AGAAATGTTC ATTGTTCAAG 
CCACTCGATG AAACAGAAAA AAGATGCCAA GTTTATCAAA GAAGAGTGGT GTTAACTACT 
AGCTCTGGAG AGTTTTTGAT CTGGAGCAAC TCAGTCAGAT TTGTTATCGA TGTGGGTGTG 
GAAAGAAGAA AGGTGTACAA CCCGAGAATA AGAGCAAACT CGCTCGTCAT GCAGCCCATC 
AGCCAGAGCC AGGCAGAGAT ACGCAAGCAG ATTCTTGGCT CATCTTCTTC AGGAAAATTT 
TTCTGCCTGT ACACTGAAGA ATTTGCCTCC AAAGACATGA CGCCACTGAA GCCAGCAGAA 
ATGCAGGAAG CCAACCTAAC AAGCATGGTG CTTTTTATGA AGAGGATAGA CATTGCGGGC 
CTAGGCCACT GTGACTTCAT GAACAGACCA GCACCAGAAA GTTTGATGCA GGCATTGGAA 
GACTTAGATT ATCTGGCAGC ACTGGATAAT GATGGAAATC TTTCTGAATT TGGAATCATC 
ATGTCAGAGT TTCCTCTTGA TCCACAACTC TCGAAGTCTA TCTTAGCGTC CTGTGAATTT 
GACTGTGTAG ATGAAGTGCT AACAATCGCA GCCATGGTAA CAGCTCCAAA TTGCTTTTCA 
CATGTGCCAC ATGGAGCTGA AGAGGCTGCC TTGACTTGTT GGAAGACATT TTTACATCCC 
GAAGGAGATC ACTTTACCCT CATCAGCATT TACAAGGCTT ACCAAGACAC AACTCTGAAT 
TCTAGCAGTG AGTACTGTGT GGAAAAGTGG TGTCGTGATT ACTTCCTCAA CTGTTCAGCA 
CTCAGAATGG CAGATGTTAT TCGAGCTGAA CTCTTAGAAA TTATCAAGCG AATCGAGCTT 
CCCTATGCAG AACCTGCTTT TGGCTCCAAG GAAAACACTC TAAACATAAA GAAAGCTCTT 
CTGTCCGGTT ACTTTATGCA GATTGCTCGG GATGTTGATG GATCAGGTAA CTACTTAATG 
CTGACACATA AGCAGGTTGC TCAGCTGCAT CCCCTGTCTG GTTACTCAAT CACCAAGAAG 
ATGCCAGAGT GGGTCCTCTT CCATAAATTC AGCATTTCTG AGAACAACTA CATCAGGATT 
ACCTCAGAAA TCTCTCCTGA ACTATTTATG CAGCTGGTAC CACAATACTA TTTCAGTAAT 
CTGCCTCCTA GTGAAAGTAA GGACATTCTA CAGCAAGTAG TGGATCACCT ATCCCCTGTG 
TCAACAATGA ATAAGGAACA GCAAATGTGT GAGACGTGCC CTGAAACTGA ACAGAGATGC 
ACTCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting ?-catenin Signaling to Induce Expression of VEGFA.
EBioMedicine1862-72(2017 Apr)
Lin H,Fang Z,Su Y,Li P,Wang J,Liao H,Hu Q,Ye C,Fang Y,Luo Q,Lin Z,Pan C,Wang F,Zhang ZY

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer.
Journal of experimental & clinical cancer research : CR2811(2009 Jan)
Huang C,Liang X,Huang R,Zhang Z

Altered distribution of heat shock protein 60 (Hsp60) with dysregulated expression of DHX32.
Experimental and molecular pathology82(3)256-61(2007 Jun)
Chen Y,Alli Z,Ackerley C,Al-Saud B,Abdelhaleem M

A role for DHX32 in regulating T-cell apoptosis.
Anticancer research27(1A)373-7(2007 Jan-Feb)
Alli Z,Chen Y,Abdul Wajid S,Al-Saud B,Abdelhaleem M

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DHX32 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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