NUP133 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP133
Entrez Gene ID	55746
Full Name	nucleoporin 133
Synonyms	hNUP133
General protein information	Preferred Names nucleoporin 133 Names nuclear pore complex protein Nup133 133 kDa nucleoporin nucleoporin 133kD nucleoporin 133kDa nucleoporin Nup133
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.13
Summary	The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008].
Disorder MIM:	607613

mRNA and Protein(s)

mRNA	Protein	Name
NM_018230.2	NP_060700.2	nuclear pore complex protein Nup133
NM_018230.3	NP_060700.2	nuclear pore complex protein Nup133

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NUP133	XP_857319.1
Drosophila melanogaster (fruit fly)	Nup133	NP_651080.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003503	XP_309655.5
	NUP133	XP_002802169.1
Homo sapiens (human)	NUP133	NP_060700.2
Mus musculus (house mouse)	Nup133	NP_758492.2
Gallus gallus (chicken)	NUP133	XP_419576.4
Pan troglodytes (chimpanzee)	NUP133	XP_003308855.1
Bos taurus (cattle)	NUP133	NP_001070340.1
Rattus norvegicus (Norway rat)	Nup133	XP_006255881.1
Danio rerio (zebrafish)	nup133	NP_998696.1
Xenopus tropicalis (tropical clawed frog)	nup133	XP_002932582.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic Protein-Protein Interaction Analysis Reveals Intersubcomplex Contacts in the Nuclear Pore Complex.
Apelt L, Knockenhauer KE, Leksa NC, Benlasfer N, Schwartz TU, Stelzl U
Molecular & cellular proteomics : MCP15(8)2594-606(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Data present crystal structures of yNup170(979-1502) and hNup107(658-925) x hNup133(517-1156), and conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor.
Title: Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element.

The significant topological differences between Nup107 and Nup133 suggest that helical nucleoporin domains of the nuclear pore complex scaffold fall in different classes and fulfill largely nonredundant functions.
Title: Structural and functional studies of Nup107/Nup133 interaction and its implications for the architecture of the nuclear pore complex.

When complexed with NUP107, this complex will give the first insights into the protein-protein interactions within a core module of the nuclear pore complex.
Title: Purification, crystallization and preliminary X-ray analysis of a Nup107-Nup133 heterodimeric nucleoporin complex.

The following NUP133 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP133 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26548	NM_018230.3 Latest version!	Homo sapiens nucleoporin 133 (NUP133), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26548	NM_018230.2	Homo sapiens nucleoporin 133 (NUP133), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP133 ( NM_018230.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060700.2 Homo sapiens nucleoporin 133 (NUP133), mRNA.

Price & Availability↑

CloneID	OHu26548
Clone ID Related Accession (Same CDS sequence)	NM_018230.2 , NM_018230.3
Accession Version	NM_018230.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup133
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK001676.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2002 this sequence version replaced NM_018230.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001676.1, SRR1660803.190645.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCAG CCGCCCCTTC TCCGCGGACC CCGGGTACCG GGTCCCGAAG GGGCCCGCTG 
GCCGGACTCG GGCCCGGCTC CACGCCCCGG ACGGCTAGCA GGAAGGGTCT GCCCCTGGGG 
TCTGCAGTCA GCTCCCCAGT GCTCTTCTCG CCGGTCGGCC GGCGTAGCTC GCTAAGCTCG 
CGGGGAACAC CAACACGAAT GTTCCCACAC CACTCCATAA CTGAGTCTGT GAACTATGAT 
GTGAAAACGT TTGGATCTTC TCTTCCTGTT AAAGTCATGG AAGCCCTAAC ATTGGCTGAA 
GTCGATGACC AGCTGACCAT TAACATAGAT GAAGGTGGAT GGGCTTGTCT GGTGTGCAAA 
GAGAAGCTCA TTATTTGGAA GATTGCTCTG TCACCTATTA CTAAGTTATC CGTTTGCAAA 
GAACTTCAGC TGCCACCTAG TGATTTCCAC TGGAGTGCCG ACTTAGTGGC TCTTTCTTAC 
TCTTCTCCCT CAGGTGAAGC ACATTCTACT CAGGCTGTTG CTGTCATGGT TGCCACCAGA 
GAAGGATCTA TCCGCTATTG GCCAAGCCTT GCTGGTGAAG ATACCTACAC AGAGGCTTTT 
GTAGATTCGG GAGGTGATAA GACTTACAGT TTCCTAACAG CAGTGCAGGG AGGAAGTTTT 
ATTTTGTCTT CATCAGGAAG CCAACTAATT CGGTTGATAC CTGAGAGCTC AGGAAAGATT 
CATCAGCATA TCCTGCCTCA GGGGCAAGGC ATGCTTTCAG GAATTGGTCG AAAAGTTTCT 
TCTCTTTTTG GAATTTTATC TCCTAGTAGT GATCTCACAC TTTCAAGTGT TCTCTGGGAT 
AGAGAGAGAT CAAGCTTTTA TAGCCTGACG AGTTCAAACA TCAGTAAATG GGAATTAGAT 
GATTCTTCAG AAAAGCATGC ATACAGTTGG GATATAAATA GAGCCCTGAA GGAAAACATT 
ACCGATGCTA TTTGGGGATC TGAAAGTAAC TATGAAGCTA TTAAAGAAGG AGTCAACATT 
CGATATTTGG ACTTGAAGCA AAACTGTGAT GGGCTGGTGA TTTTGGCAGC AGCATGGCAC 
TCAGCAGACA ATCCATGTCT CATCTATTAC TCTCTGATAA CAATAGAAGA TAATGGTTGC 
CAAATGTCAG ATGCAGTTAC TGTAGAAGTC ACTCAATATA ATCCACCTTT TCAGTCTGAA 
GACCTGATTT TGTGTCAGTT GACGGTCCCA AACTTTTCAA ACCAGACTGC CTATCTGTAT 
AACGAAAGTG CTGTCTATGT GTGCTCCACA GGAACTGGGA AATTTTCTCT TCCCCAGGAG 
AAAATTGTCT TTAATGCACA AGGAGATAGT GTTTTAGGTG CTGGTGCCTG TGGTGGTGTT 
CCTATCATTT TTTCTAGAAA CAGTGGACTG GTGTCTATTA CTTCAAGGGA AAATGTGTCT 
ATATTGGCAG AAGACTTGGA AGGGTCTTTA GCATCTTCAG TTGCTGGACC AAACAGTGAG 
AGTATGATTT TTGAGACCAC TACAAAGAAT GAAACTATAG CCCAGGAAGA TAAAATCAAG 
TTGCTGAAAG CTGCCTTTCT GCAATACTGC AGAAAAGATT TAGGTCATGC TCAAATGGTG 
GTTGATGAGC TCTTTTCCTC TCACTCTGAT TTGGATTCTG ATTCTGAACT AGACAGGGCA 
GTTACCCAAA TCAGTGTAGA CCTGATGGAT GACTACCCAG CATCTGACCC ACGGTGGGCT 
GAGTCTGTCC CTGAGGAAGC ACCTGGGTTC AGCAATACGT CACTGATTAT CCTTCACCAG 
CTAGAAGACA AGATGAAAGC TCACTCTTTT CTTATGGACT TTATTCATCA AGTTGGCTTA 
TTTGGACGTC TAGGCAGTTT TCCAGTTAGA GGGACACCGA TGGCCACTCG ACTGTTGCTC 
TGTGAGCATG CCGAAAAGCT GTCAGCCGCC ATTGTTCTCA AGAACCACCA CTCCCGGCTT 
TCTGACCTTG TCAACACAGC CATATTGATT GCTTTGAACA AGAGGGAGTA TGAAATCCCA 
TCCAACCTGA CTCCTGCAGA TGTCTTTTTC AGGGAGGTAT CCCAAGTAGA TACCATCTGT 
GAGTGCTTAC TGGAGCATGA GGAGCAAGTC TTGAGGGATG CACCTATGGA TTCCATTGAA 
TGGGCTGAAG TGGTGATCAA TGTGAACAAT ATTCTCAAGG ATATGCTGCA GGCTGCTAGT 
CATTATCGCC AAAATAGAAA CTCTTTGTAT AGAAGAGAAG AATCACTAGA AAAAGAACCT 
GAATATGTTC CATGGACGGC AACAAGTGGT CCTGGTGGCA TCCGAACGGT AATAATACGC 
CAGCATGAGA TTGTCCTGAA GGTGGCTTAT CCACAGGCAG ACAGCAACCT CCGAAACATC 
GTGACCGAGC AGCTGGTAGC CCTGATCGAT TGCTTCCTGG ATGGTTATGT TTCTCAGCTT 
AAGTCTGTGG ATAAATCCAG TAATCGGGAA AGATATGACA ATCTGGAGAT GGAATACCTA 
CAGAAAAGAT CAGATCTCTT ATCTCCTCTT CTTTCACTAG GCCAGTACCT GTGGGCTGCT 
TCTCTAGCAG AGAAATACTG TGACTTTGAT ATATTGGTAC AAATGTGTGA GCAGACTGAC 
AACCAGAGCC GACTCCAGCG CTACATGACC CAGTTTGCTG ATCAGAATTT TTCAGACTTT 
CTCTTCCGTT GGTATCTGGA GAAAGGAAAG CGAGGCAAAT TATTATCTCA GCCCATTTCT 
CAGCATGGAC AGTTGGCAAA TTTTTTGCAA GCTCATGAAC ATCTCAGCTG GTTACATGAA 
ATTAATAGCC AAGAATTAGA AAAGGCTCAT GCAACACTTC TGGGTTTGGC AAATATGGAA 
ACTCGTTACT TTGCAAAGAA GAAAACCCTT CTTGGCTTGA GTAAATTGGC TGCATTAGCT 
TCAGACTTTT CAGAGGATAT GCTACAAGAA AAAATTGAAG AAATGGCTGA GCAGGAGCGC 
TTTCTACTGC ATCAGGAGAC CCTACCTGAA CAGCTGCTGG CGGAGAAACA GCTAAATCTC 
AGTGCGATGC CAGTATTGAC TGCACCACAA CTCATTGGTC TATATATCTG TGAAGAAAAT 
AGAAGAGCTA ATGAATATGA TTTCAAGAAA GCTTTGGACT TGTTGGAATA TATTGATGAG 
GAAGAAGATA TAAATATAAA TGATCTAAAA CTGGAAATCC TTTGCAAAGC TCTTCAGAGA 
GATAACTGGT CCAGTTCTGA TGGCAAAGAT GATCCAATTG AAGTATCTAA AGACAGTATA 
TTTGTGAAGA TCTTACAGAA ACTTTTAAAA GATGGCATTC AGCTCAGTGA GTACTTACCG 
GAGGTGAAAG ACCTGCTACA AGCGGATCAG CTTGGAAGCT TAAAGTCCAA TCCTTACTTC 
GAGTTTGTTT TGAAAGCAAA TTATGAATAT TATGTTCAGG GACAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060700.2
CDS	93..3563
Translation	MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSSRGTPTRMFPHHSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCKEKLIIWKIALSPITKLSVCKELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATREGSIRYWPSLAGEDTYTEAFVDSGGDKTYSFLTAVQGGSFILSSSGSQLIRLIPESSGKIHQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWDRERSSFYSLTSSNISKWELDDSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDLKQNCDGLVILAAAWHSADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLYNESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVSILAEDLEGSLASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMVVDELFSSHSDLDSDSELDRAVTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQLEDKMKAHSFLMDFIHQVGLFGRLGSFPVRGTPMATRLLLCEHAEKLSAAIVLKNHHSRLSDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTICECLLEHEEQVLRDAPMDSIEWAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYLQKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGKRGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANMETRYFAKKKTLLGLSKLAALASDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNLSAMPVLTAPQLIGLYICEENRRANEYDFKKALDLLEYIDEEEDININDLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQI

Target ORF information:

RefSeq Version	NM_018230.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 133 (NUP133), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018230.2

ORF Insert Sequence:

ATGTTCCCAG CCGCCCCTTC TCCGCGGACC CCGGGTACCG GGTCCCGAAG GGGCCCGCTG 
GCCGGACTCG GGCCCGGCTC CACGCCCCGG ACGGCTAGCA GGAAGGGTCT GCCCCTGGGG 
TCTGCAGTCA GCTCCCCAGT GCTCTTCTCG CCGGTCGGCC GGCGTAGCTC GCTAAGCTCG 
CGGGGAACAC CAACACGAAT GTTCCCACAC CACTCCATAA CTGAGTCTGT GAACTATGAT 
GTGAAAACGT TTGGATCTTC TCTTCCTGTT AAAGTCATGG AAGCCCTAAC ATTGGCTGAA 
GTCGATGACC AGCTGACCAT TAACATAGAT GAAGGTGGAT GGGCTTGTCT GGTGTGCAAA 
GAGAAGCTCA TTATTTGGAA GATTGCTCTG TCACCTATTA CTAAGTTATC CGTTTGCAAA 
GAACTTCAGC TGCCACCTAG TGATTTCCAC TGGAGTGCCG ACTTAGTGGC TCTTTCTTAC 
TCTTCTCCCT CAGGTGAAGC ACATTCTACT CAGGCTGTTG CTGTCATGGT TGCCACCAGA 
GAAGGATCTA TCCGCTATTG GCCAAGCCTT GCTGGTGAAG ATACCTACAC AGAGGCTTTT 
GTAGATTCGG GAGGTGATAA GACTTACAGT TTCCTAACAG CAGTGCAGGG AGGAAGTTTT 
ATTTTGTCTT CATCAGGAAG CCAACTAATT CGGTTGATAC CTGAGAGCTC AGGAAAGATT 
CATCAGCATA TCCTGCCTCA GGGGCAAGGC ATGCTTTCAG GAATTGGTCG AAAAGTTTCT 
TCTCTTTTTG GAATTTTATC TCCTAGTAGT GATCTCACAC TTTCAAGTGT TCTCTGGGAT 
AGAGAGAGAT CAAGCTTTTA TAGCCTGACG AGTTCAAACA TCAGTAAATG GGAATTAGAT 
GATTCTTCAG AAAAGCATGC ATACAGTTGG GATATAAATA GAGCCCTGAA GGAAAACATT 
ACCGATGCTA TTTGGGGATC TGAAAGTAAC TATGAAGCTA TTAAAGAAGG AGTCAACATT 
CGATATTTGG ACTTGAAGCA AAACTGTGAT GGGCTGGTGA TTTTGGCAGC AGCATGGCAC 
TCAGCAGACA ATCCATGTCT CATCTATTAC TCTCTGATAA CAATAGAAGA TAATGGTTGC 
CAAATGTCAG ATGCAGTTAC TGTAGAAGTC ACTCAATATA ATCCACCTTT TCAGTCTGAA 
GACCTGATTT TGTGTCAGTT GACGGTCCCA AACTTTTCAA ACCAGACTGC CTATCTGTAT 
AACGAAAGTG CTGTCTATGT GTGCTCCACA GGAACTGGGA AATTTTCTCT TCCCCAGGAG 
AAAATTGTCT TTAATGCACA AGGAGATAGT GTTTTAGGTG CTGGTGCCTG TGGTGGTGTT 
CCTATCATTT TTTCTAGAAA CAGTGGACTG GTGTCTATTA CTTCAAGGGA AAATGTGTCT 
ATATTGGCAG AAGACTTGGA AGGGTCTTTA GCATCTTCAG TTGCTGGACC AAACAGTGAG 
AGTATGATTT TTGAGACCAC TACAAAGAAT GAAACTATAG CCCAGGAAGA TAAAATCAAG 
TTGCTGAAAG CTGCCTTTCT GCAATACTGC AGAAAAGATT TAGGTCATGC TCAAATGGTG 
GTTGATGAGC TCTTTTCCTC TCACTCTGAT TTGGATTCTG ATTCTGAACT AGACAGGGCA 
GTTACCCAAA TCAGTGTAGA CCTGATGGAT GACTACCCAG CATCTGACCC ACGGTGGGCT 
GAGTCTGTCC CTGAGGAAGC ACCTGGGTTC AGCAATACGT CACTGATTAT CCTTCACCAG 
CTAGAAGACA AGATGAAAGC TCACTCTTTT CTTATGGACT TTATTCATCA AGTTGGCTTA 
TTTGGACGTC TAGGCAGTTT TCCAGTTAGA GGGACACCGA TGGCCACTCG ACTGTTGCTC 
TGTGAGCATG CCGAAAAGCT GTCAGCCGCC ATTGTTCTCA AGAACCACCA CTCCCGGCTT 
TCTGACCTTG TCAACACAGC CATATTGATT GCTTTGAACA AGAGGGAGTA TGAAATCCCA 
TCCAACCTGA CTCCTGCAGA TGTCTTTTTC AGGGAGGTAT CCCAAGTAGA TACCATCTGT 
GAGTGCTTAC TGGAGCATGA GGAGCAAGTC TTGAGGGATG CACCTATGGA TTCCATTGAA 
TGGGCTGAAG TGGTGATCAA TGTGAACAAT ATTCTCAAGG ATATGCTGCA GGCTGCTAGT 
CATTATCGCC AAAATAGAAA CTCTTTGTAT AGAAGAGAAG AATCACTAGA AAAAGAACCT 
GAATATGTTC CATGGACGGC AACAAGTGGT CCTGGTGGCA TCCGAACGGT AATAATACGC 
CAGCATGAGA TTGTCCTGAA GGTGGCTTAT CCACAGGCAG ACAGCAACCT CCGAAACATC 
GTGACCGAGC AGCTGGTAGC CCTGATCGAT TGCTTCCTGG ATGGTTATGT TTCTCAGCTT 
AAGTCTGTGG ATAAATCCAG TAATCGGGAA AGATATGACA ATCTGGAGAT GGAATACCTA 
CAGAAAAGAT CAGATCTCTT ATCTCCTCTT CTTTCACTAG GCCAGTACCT GTGGGCTGCT 
TCTCTAGCAG AGAAATACTG TGACTTTGAT ATATTGGTAC AAATGTGTGA GCAGACTGAC 
AACCAGAGCC GACTCCAGCG CTACATGACC CAGTTTGCTG ATCAGAATTT TTCAGACTTT 
CTCTTCCGTT GGTATCTGGA GAAAGGAAAG CGAGGCAAAT TATTATCTCA GCCCATTTCT 
CAGCATGGAC AGTTGGCAAA TTTTTTGCAA GCTCATGAAC ATCTCAGCTG GTTACATGAA 
ATTAATAGCC AAGAATTAGA AAAGGCTCAT GCAACACTTC TGGGTTTGGC AAATATGGAA 
ACTCGTTACT TTGCAAAGAA GAAAACCCTT CTTGGCTTGA GTAAATTGGC TGCATTAGCT 
TCAGACTTTT CAGAGGATAT GCTACAAGAA AAAATTGAAG AAATGGCTGA GCAGGAGCGC 
TTTCTACTGC ATCAGGAGAC CCTACCTGAA CAGCTGCTGG CGGAGAAACA GCTAAATCTC 
AGTGCGATGC CAGTATTGAC TGCACCACAA CTCATTGGTC TATATATCTG TGAAGAAAAT 
AGAAGAGCTA ATGAATATGA TTTCAAGAAA GCTTTGGACT TGTTGGAATA TATTGATGAG 
GAAGAAGATA TAAATATAAA TGATCTAAAA CTGGAAATCC TTTGCAAAGC TCTTCAGAGA 
GATAACTGGT CCAGTTCTGA TGGCAAAGAT GATCCAATTG AAGTATCTAA AGACAGTATA 
TTTGTGAAGA TCTTACAGAA ACTTTTAAAA GATGGCATTC AGCTCAGTGA GTACTTACCG 
GAGGTGAAAG ACCTGCTACA AGCGGATCAG CTTGGAAGCT TAAAGTCCAA TCCTTACTTC 
GAGTTTGTTT TGAAAGCAAA TTATGAATAT TATGTTCAGG GACAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP133 ( NM_018230.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060700.2 Homo sapiens nucleoporin 133 (NUP133), mRNA.

Price & Availability↑

CloneID	OHu26548
Clone ID Related Accession (Same CDS sequence)	NM_018230.2 , NM_018230.3
Accession Version	NM_018230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	nuclear pore complex protein Nup133
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121990.34, AL139252.8 and AL160004.18. This sequence is a reference standard in the RefSeqGene project. On Nov 25, 2018 this sequence version replaced NM_018230.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001676.1, SRR1803613.135039.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261396.6/ ENSP00000261396.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCCAG CCGCCCCTTC TCCGCGGACC CCGGGTACCG GGTCCCGAAG GGGCCCGCTG 
GCCGGACTCG GGCCCGGCTC CACGCCCCGG ACGGCTAGCA GGAAGGGTCT GCCCCTGGGG 
TCTGCAGTCA GCTCCCCAGT GCTCTTCTCG CCGGTCGGCC GGCGTAGCTC GCTAAGCTCG 
CGGGGAACAC CAACACGAAT GTTCCCACAC CACTCCATAA CTGAGTCTGT GAACTATGAT 
GTGAAAACGT TTGGATCTTC TCTTCCTGTT AAAGTCATGG AAGCCCTAAC ATTGGCTGAA 
GTCGATGACC AGCTGACCAT TAACATAGAT GAAGGTGGAT GGGCTTGTCT GGTGTGCAAA 
GAGAAGCTCA TTATTTGGAA GATTGCTCTG TCACCTATTA CTAAGTTATC CGTTTGCAAA 
GAACTTCAGC TGCCACCTAG TGATTTCCAC TGGAGTGCCG ACTTAGTGGC TCTTTCTTAC 
TCTTCTCCCT CAGGTGAAGC ACATTCTACT CAGGCTGTTG CTGTCATGGT TGCCACCAGA 
GAAGGATCTA TCCGCTATTG GCCAAGCCTT GCTGGTGAAG ATACCTACAC AGAGGCTTTT 
GTAGATTCGG GAGGTGATAA GACTTACAGT TTCCTAACAG CAGTGCAGGG AGGAAGTTTT 
ATTTTGTCTT CATCAGGAAG CCAACTAATT CGGTTGATAC CTGAGAGCTC AGGAAAGATT 
CATCAGCATA TCCTGCCTCA GGGGCAAGGC ATGCTTTCAG GAATTGGTCG AAAAGTTTCT 
TCTCTTTTTG GAATTTTATC TCCTAGTAGT GATCTCACAC TTTCAAGTGT TCTCTGGGAT 
AGAGAGAGAT CAAGCTTTTA TAGCCTGACG AGTTCAAACA TCAGTAAATG GGAATTAGAT 
GATTCTTCAG AAAAGCATGC ATACAGTTGG GATATAAATA GAGCCCTGAA GGAAAACATT 
ACCGATGCTA TTTGGGGATC TGAAAGTAAC TATGAAGCTA TTAAAGAAGG AGTCAACATT 
CGATATTTGG ACTTGAAGCA AAACTGTGAT GGGCTGGTGA TTTTGGCAGC AGCATGGCAC 
TCAGCAGACA ATCCATGTCT CATCTATTAC TCTCTGATAA CAATAGAAGA TAATGGTTGC 
CAAATGTCAG ATGCAGTTAC TGTAGAAGTC ACTCAATATA ATCCACCTTT TCAGTCTGAA 
GACCTGATTT TGTGTCAGTT GACGGTCCCA AACTTTTCAA ACCAGACTGC CTATCTGTAT 
AACGAAAGTG CTGTCTATGT GTGCTCCACA GGAACTGGGA AATTTTCTCT TCCCCAGGAG 
AAAATTGTCT TTAATGCACA AGGAGATAGT GTTTTAGGTG CTGGTGCCTG TGGTGGTGTT 
CCTATCATTT TTTCTAGAAA CAGTGGACTG GTGTCTATTA CTTCAAGGGA AAATGTGTCT 
ATATTGGCAG AAGACTTGGA AGGGTCTTTA GCATCTTCAG TTGCTGGACC AAACAGTGAG 
AGTATGATTT TTGAGACCAC TACAAAGAAT GAAACTATAG CCCAGGAAGA TAAAATCAAG 
TTGCTGAAAG CTGCCTTTCT GCAATACTGC AGAAAAGATT TAGGTCATGC TCAAATGGTG 
GTTGATGAGC TCTTTTCCTC TCACTCTGAT TTGGATTCTG ATTCTGAACT AGACAGGGCA 
GTTACCCAAA TCAGTGTAGA CCTGATGGAT GACTACCCAG CATCTGACCC ACGGTGGGCT 
GAGTCTGTCC CTGAGGAAGC ACCTGGGTTC AGCAATACGT CACTGATTAT CCTTCACCAG 
CTAGAAGACA AGATGAAAGC TCACTCTTTT CTTATGGACT TTATTCATCA AGTTGGCTTA 
TTTGGACGTC TAGGCAGTTT TCCAGTTAGA GGGACACCGA TGGCCACTCG ACTGTTGCTC 
TGTGAGCATG CCGAAAAGCT GTCAGCCGCC ATTGTTCTCA AGAACCACCA CTCCCGGCTT 
TCTGACCTTG TCAACACAGC CATATTGATT GCTTTGAACA AGAGGGAGTA TGAAATCCCA 
TCCAACCTGA CTCCTGCAGA TGTCTTTTTC AGGGAGGTAT CCCAAGTAGA TACCATCTGT 
GAGTGCTTAC TGGAGCATGA GGAGCAAGTC TTGAGGGATG CACCTATGGA TTCCATTGAA 
TGGGCTGAAG TGGTGATCAA TGTGAACAAT ATTCTCAAGG ATATGCTGCA GGCTGCTAGT 
CATTATCGCC AAAATAGAAA CTCTTTGTAT AGAAGAGAAG AATCACTAGA AAAAGAACCT 
GAATATGTTC CATGGACGGC AACAAGTGGT CCTGGTGGCA TCCGAACGGT AATAATACGC 
CAGCATGAGA TTGTCCTGAA GGTGGCTTAT CCACAGGCAG ACAGCAACCT CCGAAACATC 
GTGACCGAGC AGCTGGTAGC CCTGATCGAT TGCTTCCTGG ATGGTTATGT TTCTCAGCTT 
AAGTCTGTGG ATAAATCCAG TAATCGGGAA AGATATGACA ATCTGGAGAT GGAATACCTA 
CAGAAAAGAT CAGATCTCTT ATCTCCTCTT CTTTCACTAG GCCAGTACCT GTGGGCTGCT 
TCTCTAGCAG AGAAATACTG TGACTTTGAT ATATTGGTAC AAATGTGTGA GCAGACTGAC 
AACCAGAGCC GACTCCAGCG CTACATGACC CAGTTTGCTG ATCAGAATTT TTCAGACTTT 
CTCTTCCGTT GGTATCTGGA GAAAGGAAAG CGAGGCAAAT TATTATCTCA GCCCATTTCT 
CAGCATGGAC AGTTGGCAAA TTTTTTGCAA GCTCATGAAC ATCTCAGCTG GTTACATGAA 
ATTAATAGCC AAGAATTAGA AAAGGCTCAT GCAACACTTC TGGGTTTGGC AAATATGGAA 
ACTCGTTACT TTGCAAAGAA GAAAACCCTT CTTGGCTTGA GTAAATTGGC TGCATTAGCT 
TCAGACTTTT CAGAGGATAT GCTACAAGAA AAAATTGAAG AAATGGCTGA GCAGGAGCGC 
TTTCTACTGC ATCAGGAGAC CCTACCTGAA CAGCTGCTGG CGGAGAAACA GCTAAATCTC 
AGTGCGATGC CAGTATTGAC TGCACCACAA CTCATTGGTC TATATATCTG TGAAGAAAAT 
AGAAGAGCTA ATGAATATGA TTTCAAGAAA GCTTTGGACT TGTTGGAATA TATTGATGAG 
GAAGAAGATA TAAATATAAA TGATCTAAAA CTGGAAATCC TTTGCAAAGC TCTTCAGAGA 
GATAACTGGT CCAGTTCTGA TGGCAAAGAT GATCCAATTG AAGTATCTAA AGACAGTATA 
TTTGTGAAGA TCTTACAGAA ACTTTTAAAA GATGGCATTC AGCTCAGTGA GTACTTACCG 
GAGGTGAAAG ACCTGCTACA AGCGGATCAG CTTGGAAGCT TAAAGTCCAA TCCTTACTTC 
GAGTTTGTTT TGAAAGCAAA TTATGAATAT TATGTTCAGG GACAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060700.2
CDS	93..3563
Translation	MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSSRGTPTRMFPHHSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCKEKLIIWKIALSPITKLSVCKELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATREGSIRYWPSLAGEDTYTEAFVDSGGDKTYSFLTAVQGGSFILSSSGSQLIRLIPESSGKIHQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWDRERSSFYSLTSSNISKWELDDSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDLKQNCDGLVILAAAWHSADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLYNESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVSILAEDLEGSLASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMVVDELFSSHSDLDSDSELDRAVTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQLEDKMKAHSFLMDFIHQVGLFGRLGSFPVRGTPMATRLLLCEHAEKLSAAIVLKNHHSRLSDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTICECLLEHEEQVLRDAPMDSIEWAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYLQKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGKRGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANMETRYFAKKKTLLGLSKLAALASDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNLSAMPVLTAPQLIGLYICEENRRANEYDFKKALDLLEYIDEEEDININDLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQI

Target ORF information:

RefSeq Version	NM_018230.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleoporin 133 (NUP133), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018230.3

ORF Insert Sequence:

ATGTTCCCAG CCGCCCCTTC TCCGCGGACC CCGGGTACCG GGTCCCGAAG GGGCCCGCTG 
GCCGGACTCG GGCCCGGCTC CACGCCCCGG ACGGCTAGCA GGAAGGGTCT GCCCCTGGGG 
TCTGCAGTCA GCTCCCCAGT GCTCTTCTCG CCGGTCGGCC GGCGTAGCTC GCTAAGCTCG 
CGGGGAACAC CAACACGAAT GTTCCCACAC CACTCCATAA CTGAGTCTGT GAACTATGAT 
GTGAAAACGT TTGGATCTTC TCTTCCTGTT AAAGTCATGG AAGCCCTAAC ATTGGCTGAA 
GTCGATGACC AGCTGACCAT TAACATAGAT GAAGGTGGAT GGGCTTGTCT GGTGTGCAAA 
GAGAAGCTCA TTATTTGGAA GATTGCTCTG TCACCTATTA CTAAGTTATC CGTTTGCAAA 
GAACTTCAGC TGCCACCTAG TGATTTCCAC TGGAGTGCCG ACTTAGTGGC TCTTTCTTAC 
TCTTCTCCCT CAGGTGAAGC ACATTCTACT CAGGCTGTTG CTGTCATGGT TGCCACCAGA 
GAAGGATCTA TCCGCTATTG GCCAAGCCTT GCTGGTGAAG ATACCTACAC AGAGGCTTTT 
GTAGATTCGG GAGGTGATAA GACTTACAGT TTCCTAACAG CAGTGCAGGG AGGAAGTTTT 
ATTTTGTCTT CATCAGGAAG CCAACTAATT CGGTTGATAC CTGAGAGCTC AGGAAAGATT 
CATCAGCATA TCCTGCCTCA GGGGCAAGGC ATGCTTTCAG GAATTGGTCG AAAAGTTTCT 
TCTCTTTTTG GAATTTTATC TCCTAGTAGT GATCTCACAC TTTCAAGTGT TCTCTGGGAT 
AGAGAGAGAT CAAGCTTTTA TAGCCTGACG AGTTCAAACA TCAGTAAATG GGAATTAGAT 
GATTCTTCAG AAAAGCATGC ATACAGTTGG GATATAAATA GAGCCCTGAA GGAAAACATT 
ACCGATGCTA TTTGGGGATC TGAAAGTAAC TATGAAGCTA TTAAAGAAGG AGTCAACATT 
CGATATTTGG ACTTGAAGCA AAACTGTGAT GGGCTGGTGA TTTTGGCAGC AGCATGGCAC 
TCAGCAGACA ATCCATGTCT CATCTATTAC TCTCTGATAA CAATAGAAGA TAATGGTTGC 
CAAATGTCAG ATGCAGTTAC TGTAGAAGTC ACTCAATATA ATCCACCTTT TCAGTCTGAA 
GACCTGATTT TGTGTCAGTT GACGGTCCCA AACTTTTCAA ACCAGACTGC CTATCTGTAT 
AACGAAAGTG CTGTCTATGT GTGCTCCACA GGAACTGGGA AATTTTCTCT TCCCCAGGAG 
AAAATTGTCT TTAATGCACA AGGAGATAGT GTTTTAGGTG CTGGTGCCTG TGGTGGTGTT 
CCTATCATTT TTTCTAGAAA CAGTGGACTG GTGTCTATTA CTTCAAGGGA AAATGTGTCT 
ATATTGGCAG AAGACTTGGA AGGGTCTTTA GCATCTTCAG TTGCTGGACC AAACAGTGAG 
AGTATGATTT TTGAGACCAC TACAAAGAAT GAAACTATAG CCCAGGAAGA TAAAATCAAG 
TTGCTGAAAG CTGCCTTTCT GCAATACTGC AGAAAAGATT TAGGTCATGC TCAAATGGTG 
GTTGATGAGC TCTTTTCCTC TCACTCTGAT TTGGATTCTG ATTCTGAACT AGACAGGGCA 
GTTACCCAAA TCAGTGTAGA CCTGATGGAT GACTACCCAG CATCTGACCC ACGGTGGGCT 
GAGTCTGTCC CTGAGGAAGC ACCTGGGTTC AGCAATACGT CACTGATTAT CCTTCACCAG 
CTAGAAGACA AGATGAAAGC TCACTCTTTT CTTATGGACT TTATTCATCA AGTTGGCTTA 
TTTGGACGTC TAGGCAGTTT TCCAGTTAGA GGGACACCGA TGGCCACTCG ACTGTTGCTC 
TGTGAGCATG CCGAAAAGCT GTCAGCCGCC ATTGTTCTCA AGAACCACCA CTCCCGGCTT 
TCTGACCTTG TCAACACAGC CATATTGATT GCTTTGAACA AGAGGGAGTA TGAAATCCCA 
TCCAACCTGA CTCCTGCAGA TGTCTTTTTC AGGGAGGTAT CCCAAGTAGA TACCATCTGT 
GAGTGCTTAC TGGAGCATGA GGAGCAAGTC TTGAGGGATG CACCTATGGA TTCCATTGAA 
TGGGCTGAAG TGGTGATCAA TGTGAACAAT ATTCTCAAGG ATATGCTGCA GGCTGCTAGT 
CATTATCGCC AAAATAGAAA CTCTTTGTAT AGAAGAGAAG AATCACTAGA AAAAGAACCT 
GAATATGTTC CATGGACGGC AACAAGTGGT CCTGGTGGCA TCCGAACGGT AATAATACGC 
CAGCATGAGA TTGTCCTGAA GGTGGCTTAT CCACAGGCAG ACAGCAACCT CCGAAACATC 
GTGACCGAGC AGCTGGTAGC CCTGATCGAT TGCTTCCTGG ATGGTTATGT TTCTCAGCTT 
AAGTCTGTGG ATAAATCCAG TAATCGGGAA AGATATGACA ATCTGGAGAT GGAATACCTA 
CAGAAAAGAT CAGATCTCTT ATCTCCTCTT CTTTCACTAG GCCAGTACCT GTGGGCTGCT 
TCTCTAGCAG AGAAATACTG TGACTTTGAT ATATTGGTAC AAATGTGTGA GCAGACTGAC 
AACCAGAGCC GACTCCAGCG CTACATGACC CAGTTTGCTG ATCAGAATTT TTCAGACTTT 
CTCTTCCGTT GGTATCTGGA GAAAGGAAAG CGAGGCAAAT TATTATCTCA GCCCATTTCT 
CAGCATGGAC AGTTGGCAAA TTTTTTGCAA GCTCATGAAC ATCTCAGCTG GTTACATGAA 
ATTAATAGCC AAGAATTAGA AAAGGCTCAT GCAACACTTC TGGGTTTGGC AAATATGGAA 
ACTCGTTACT TTGCAAAGAA GAAAACCCTT CTTGGCTTGA GTAAATTGGC TGCATTAGCT 
TCAGACTTTT CAGAGGATAT GCTACAAGAA AAAATTGAAG AAATGGCTGA GCAGGAGCGC 
TTTCTACTGC ATCAGGAGAC CCTACCTGAA CAGCTGCTGG CGGAGAAACA GCTAAATCTC 
AGTGCGATGC CAGTATTGAC TGCACCACAA CTCATTGGTC TATATATCTG TGAAGAAAAT 
AGAAGAGCTA ATGAATATGA TTTCAAGAAA GCTTTGGACT TGTTGGAATA TATTGATGAG 
GAAGAAGATA TAAATATAAA TGATCTAAAA CTGGAAATCC TTTGCAAAGC TCTTCAGAGA 
GATAACTGGT CCAGTTCTGA TGGCAAAGAT GATCCAATTG AAGTATCTAA AGACAGTATA 
TTTGTGAAGA TCTTACAGAA ACTTTTAAAA GATGGCATTC AGCTCAGTGA GTACTTACCG 
GAGGTGAAAG ACCTGCTACA AGCGGATCAG CTTGGAAGCT TAAAGTCCAA TCCTTACTTC 
GAGTTTGTTT TGAAAGCAAA TTATGAATAT TATGTTCAGG GACAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic Protein-Protein Interaction Analysis Reveals Intersubcomplex Contacts in the Nuclear Pore Complex.
Molecular & cellular proteomics : MCP15(8)2594-606(2016 08)
Apelt L,Knockenhauer KE,Leksa NC,Benlasfer N,Schwartz TU,Stelzl U

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NUP133 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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