IARS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS2
Entrez Gene ID	55699
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
Synonyms	CAGSSS,ILERS
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucine--tRNA ligase, mitochondrial isoleucine tRNA ligase 2, mitochondrial isoleucine-tRNA synthetase 2, mitochondrial isoleucyl-tRNA synthetase, mitochondrial mitochondrial isoleucine tRNA synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q41
Summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014].
Disorder MIM:	612801
Disorder Html:	?Cataracts, growth hormone deficiency, sensory neuropathy, sensorineural hearing loss, and skeletal dysplasia, 616007 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018060.4	NP_060530.3	isoleucine--tRNA ligase, mitochondrial precursor
NM_018060.3	NP_060530.3	isoleucine--tRNA ligase, mitochondrial precursor

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Neurospora crassa	NCU00920	XP_964746.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
	IARS2	XP_001099877.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology
Bibliography

Related articles in PubMed

Recessive Mutation in a Nuclear-Encoded Mitochondrial tRNA Synthetase Associated With Infantile Cataract, Congenital Neurotrophic Keratitis, and Orbital Myopathy.
Jabbour S, Harissi-Dagher M
Cornea35(6)894-6(2016 Jun)

IARS2 silencing induces non-small cell lung cancer cells proliferation inhibition, cell cycle arrest and promotes cell apoptosis.
Yin J, Liu W, Li R, Liu J, Zhang Y, Tang W, Wang K
Neoplasma63(1)64-71(2016)

Expression of IARS2 gene in colon cancer and effect of its knockdown on biological behavior of RKO cells.
Zhong L, Zhang Y, Yang JY, Xiong LF, Shen T, Sa YL, O'Yang YM, Zhao SH, Chen JY
International journal of clinical and experimental pathology8(10)12151-9(2015)

Mutation in the nuclear-encoded mitochondrial isoleucyl-tRNA synthetase IARS2 in patients with cataracts, growth hormone deficiency with short stature, partial sensorineural deafness, and peripheral neuropathy or with Leigh syndrome.
Schwartzentruber J, Buhas D, Majewski J, Sasarman F, Papillon-Cavanagh S, Thiffault I, Thiffaut I, Sheldon KM, Massicotte C, Patry L, Simon M, Zare AS, McKernan KJ, , Michaud J, Boles RG, Deal CL, Desilets V, Shoubridge EA, Samuels ME
Human mutation35(11)1285-9(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Patients with this very rare mutation present with a myriad of ocular findings, including infantile cataract, neurotrophic keratitis, corneal opacification, and orbital myopathy.
Title: Recessive Mutation in a Nuclear-Encoded Mitochondrial tRNA Synthetase Associated With Infantile Cataract, Congenital Neurotrophic Keratitis, and Orbital Myopathy.

The expression of IARS2 gene is different in human colon cancer and surrounding tissues. IARS2 gene is probably a cancer-promoting gene
Title: Expression of IARS2 gene in colon cancer and effect of its knockdown on biological behavior of RKO cells.

This study is the first report of clinical findings associated with IARS2 mutations.
Title: Mutation in the nuclear-encoded mitochondrial isoleucyl-tRNA synthetase IARS2 in patients with cataracts, growth hormone deficiency with short stature, partial sensorineural deafness, and peripheral neuropathy or with Leigh syndrome.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Meta-analysis of gene-disease association. (HuGE Navigator)
Title: Genetic association analysis of 13 nuclear-encoded mitochondrial candidate genes with type II diabetes mellitus: the DAMAGE study.

The following IARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13110	NM_018060.4 Latest version!	Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
View Old Accession Versions >>
OHu13110	NM_018060.3	Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS2 ( NM_018060.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060530.3 Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Price & Availability↑

CloneID	OHu13110
Clone ID Related Accession (Same CDS sequence)	NM_018060.4 , NM_018060.3
Accession Version	NM_018060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI459732.1, BM149097.1 and BC040376.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018060.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.201635.1, SRR1803613.251205.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000366922.3/ ENSP00000355889.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CGCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAACGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCATTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTT 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACGGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTG CCCATGGATT GTCTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CGTATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACGGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGGCG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACCAAG 
GATGAATACT TGATCAACAG CCAAACCACT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAACAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGCG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGATGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTAAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAACAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATA GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTT GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCACTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCCCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060530.3
CDS	86..3124
Translation	MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

Target ORF information:

RefSeq Version	NM_018060.4
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018060.4

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CGCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAACGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCATTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTT 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACGGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTG CCCATGGATT GTCTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CGTATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACGGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGGCG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACCAAG 
GATGAATACT TGATCAACAG CCAAACCACT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAACAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGCG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGATGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTAAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAACAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATA GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTT GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCACTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCCCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS2 ( NM_018060.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060530.3 Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Price & Availability↑

CloneID	OHu13110
Clone ID Related Accession (Same CDS sequence)	NM_018060.4 , NM_018060.3
Accession Version	NM_018060.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI459732.1, BM149097.1 and BC040376.1. This sequence is a reference standard in the RefSeqGene project. On Apr 29, 2004 this sequence version replaced NM_018060.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.201635.1, SRR1803615.72928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CGCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAACGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCATTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTT 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACGGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTG CCCATGGATT GTCTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CGTATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACGGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGGCG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACCAAG 
GATGAATACT TGATCAACAG CCAAACCACT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAACAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGCG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGATGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTAAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAACAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATA GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTT GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCACTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCCCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060530.3
CDS	105..3143
Translation	MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

Target ORF information:

RefSeq Version	NM_018060.3
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018060.3

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CGCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAACGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCATTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTT 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACGGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTG CCCATGGATT GTCTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CGTATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACGGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGGCG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACCAAG 
GATGAATACT TGATCAACAG CCAAACCACT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAACAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGCG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGATGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTAAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAACAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATA GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTT GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCACTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCCCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Recessive Mutation in a Nuclear-Encoded Mitochondrial tRNA Synthetase Associated With Infantile Cataract, Congenital Neurotrophic Keratitis, and Orbital Myopathy.
Cornea35(6)894-6(2016 Jun)
Jabbour S,Harissi-Dagher M

IARS2 silencing induces non-small cell lung cancer cells proliferation inhibition, cell cycle arrest and promotes cell apoptosis.
Neoplasma63(1)64-71(2016)
Yin J,Liu W,Li R,Liu J,Zhang Y,Tang W,Wang K

Expression of IARS2 gene in colon cancer and effect of its knockdown on biological behavior of RKO cells.
International journal of clinical and experimental pathology8(10)12151-9(2015)
Zhong L,Zhang Y,Yang JY,Xiong LF,Shen T,Sa YL,O'Yang YM,Zhao SH,Chen JY

Mutation in the nuclear-encoded mitochondrial isoleucyl-tRNA synthetase IARS2 in patients with cataracts, growth hormone deficiency with short stature, partial sensorineural deafness, and peripheral neuropathy or with Leigh syndrome.
Human mutation35(11)1285-9(2014 Nov)
Schwartzentruber J,Buhas D,Majewski J,Sasarman F,Papillon-Cavanagh S,Thiffault I,Thiffaut I,Sheldon KM,Massicotte C,Patry L,Simon M,Zare AS,McKernan KJ,,Michaud J,Boles RG,Deal CL,Desilets V,Shoubridge EA,Samuels ME

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IARS2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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